TWI795735B - Automatic portable detection system for pathogenic bacteria - Google Patents

Abstract

An automatic portable detection system for pathogenic bacteria includes a water inlet and outlet device, a miniature mixing device, a detection chip, a processing device and a display module. The miniature mixing device includes a micro pump and a micro chip. The sample water and a plurality of reaction reagents are sequentially mixed in a spiral micro-mixing channel of the micro chip through the micro pump to get an analyte solution to start a color reaction. The detection chip detect the color depth and converts it to a corresponding voltage value. The processing device receives the voltage value and generatea a detection result when the color reaction is complete.

Description

Automated portable water pathogen detection system

The invention relates to a detection system for pathogenic bacteria in water, in particular to an automatic portable detection system for pathogenic bacteria in water.

At present, the common water quality testing instruments can be mainly divided into laboratory analysis method and on-site instant analysis method. The former uses polymerase chain reaction (Polymerase chain reaction, PCR) or rapid screening system, which cannot be transported due to the bulky and expensive detection equipment, and the samples must be collected by personnel and returned to the laboratory for analysis, so it is limited by the location and does not have Convenience, although its detection equipment has high performance and can obtain better sensitivity and detection limit, it cannot perform on-site real-time detection. The latter uses instruments, although there are various testing instruments on the market, but they cannot automate sampling, testing and recording. In view of this, it is the most effective way to develop a mobile, automatic detection, fast and real-time portable water pathogen detection system, which can greatly improve the productivity and competitiveness of Taiwan's aquaculture industry.

In view of the above-mentioned problems to be solved and their reasons, specifically, the present invention provides an automatic and portable detection system for pathogenic bacteria in water, which can continuously detect dozens of days. Only a very small amount of sample water is required to be mixed through the micro-mixing device, and converted into a corresponding voltage value according to the color change of the sample. When the color reaction reaches a certain level, the detection result value is generated. And the system of the present invention can upload the measured data to the server for real-time acquisition of test results.

The invention is an automatic portable water pathogen detection system, which includes a water inlet and outlet device, a micro mixing device, a detection chip, a control device and a display module. The above-mentioned water inlet and outlet device draws sample water from the water area to be tested and discharges the tested liquid after testing. The above-mentioned micro-mixing device is connected to the above-mentioned water inlet and outlet device, which includes a plurality of micro-pumps and a micro-chip with at least one spiral micro-mixing flow channel, through which the sample water and a plurality of reaction reagents are sequentially mixed at least Mix in a spiral micro-mixing flow channel to obtain the test solution. The detection chip is connected to the micro-mixing device, which includes a liquid container to be tested, a light emitting diode and a photodiode. The liquid to be tested container is used for containing the liquid to be tested. The photodiode detects the light intensity from the light emitting diode and outputs a corresponding voltage value, wherein the light emitting diode and the photodiode are respectively located on opposite sides of the liquid container to be tested. The above-mentioned control device is connected to and controls the above-mentioned water inlet and outlet device, the micro-mixing device and the detection chip, and receives the voltage value. The above-mentioned display module is connected with the above-mentioned control device for displaying water quality information.

According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes a sterilizing module connected to the control device. The sterilization module cleans and sterilizes the above-mentioned micro-mixing device and the detection chip to ensure the correctness of the detection results.

According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes a constant temperature module connected to the control device. The constant temperature module controls the temperature of the liquid to be tested at a set temperature.

According to another embodiment of the present invention, the above constant temperature module includes a temperature sensing chip and a heating chip. The temperature sensing chip senses the ambient temperature of the detection chip, and the heating plate is used to maintain the detection chip at a set temperature. That is, when the ambient temperature is lower than the set temperature, the above-mentioned heating chip will continue to heat, and when the ambient temperature of the detected chip is higher than or equal to the set temperature, the heating will be stopped.

According to another embodiment of the present invention, the detection wafer further includes exhaust holes, water inlet holes and drain holes. The vent hole is located above the container of the liquid to be tested. The above-mentioned water inlet hole and drain hole are all located below the container of the liquid to be tested.

According to another embodiment of the present invention, the above-mentioned microchip further includes a plurality of reagent inlets, respectively communicating with the sample water container and the reaction reagent container.

According to another embodiment of the present invention, the plurality of micro-pumps communicate with the sample water container, the reaction reagent container and the test liquid container respectively.

According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes an Internet of Things module for uploading water quality information to the server.

According to another embodiment of the present invention, the above water quality information can be transmitted to web pages and mobile phone applications through the server.

The present invention is a method for using an automatic portable pathogenic bacteria detection system in water, which includes: (A) a water inlet and outlet device to extract sample water from a water area to be tested. (B) The micro-mixing device mixes the sample water and the reaction reagent in a spiral micro-mixing channel to obtain the liquid to be tested and perform a color reaction. (C) The detection chip measures the liquid to be tested to obtain the corresponding voltage value (V _i ), wherein the voltage value obtained from the first detection is the initial voltage value (V ₀ ). (D) The control device judges whether V _i /V ₀ is smaller than a preset value according to the voltage value (V _i ) and the initial voltage value (V ₀ ). (E) When V _i /V ₀ is less than a preset value, water quality information is generated according to the measurement time. (F) Discharging the detected liquid to be tested. (G) Clean the micro-mixing device and the pipeline for detecting chips. (H) When the time from the last detection reaches a preset time, repeat the above steps (A)-(G), until the number of days of detection is greater than or equal to a set number of days, then stop the detection.

According to another embodiment of the present invention, the detection chip performs the first measurement of the liquid to be tested to obtain the initial voltage value (V ₀ ), and after a fixed period of time, the detection chip performs measurement again to obtain the voltage value ( V _i ).

To sum up, the present invention provides an automated portable detection system for pathogenic bacteria in water, which is a fast, automatic, real-time detection system with the function of the Internet of Things. It does not require complicated optical instruments and excessive time and manpower, and has a certain The degree of accuracy can be uploaded to the network through wireless transmission methods, and the data can be captured through the application program. Even if people are not next to the detection system, they can directly see the real-time detection data through mobile devices such as computers and mobile phones.

In addition, because the distribution of aquaculture fish farms in Taiwan is relatively scattered, the automated mobile detection system is not restricted by region, allowing users to easily conduct water quality detection and monitoring by themselves.

100:Automated portable water pathogen detection system

110: Water inlet and outlet device

111: water pump

112: Drainage help

120: micro mixing device

121:Microchip

121a: first reagent inlet

121b: Second reagent inlet

121c: sample water inlet

121d: the first spiral micro-mixing channel

121e: the second spiral micro-mixing channel

121f: outlet for the liquid to be tested

122:Micro pump

122a: The first micro pump

122b: The second micro-pump

122c: The third micro-pump

122d: The fourth micro-pump

130: Detection wafer

131: container of liquid to be tested

132: light emitting diode

133: photodiode

134: exhaust hole

135: water inlet hole

136: drainage hole

140: Constant temperature module

141: temperature sensing chip

142: heating sheet

150: Control device

160:Internet of things module

170: server

180: display module

190: Sterilization module

191: Alcohol container

192:UV lamp

193:UV lamp controller

201-220: Steps

401: first container

402: second container

403: The third container

In order to make the above and other objects, features, advantages and embodiments of the present invention more comprehensible, the accompanying drawings are described as follows:

FIG. 1 is a schematic diagram showing the relationship between devices and modules in an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 2 is a schematic diagram illustrating the interior of an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 3 is a schematic diagram of a micro-mixing device of an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 4 is a schematic diagram of a microchip of an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 5 is a schematic diagram of a detection chip of an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 6 is a front view showing the appearance of an automated portable water pathogen detection system according to an embodiment of the present invention.

FIG. 7 is a schematic flowchart illustrating a method for using an automated portable water pathogen detection system according to an embodiment of the present invention.

In order to facilitate the understanding of the features, content and advantages of the present invention, the present invention is hereby combined with the drawings and described in detail in the form of embodiments as follows, and the drawings used therein are only for illustration and auxiliary description purposes. Not necessarily the true ratio after the implementation of the present invention Therefore, the proportion and configuration relationship of the attached drawings should not be interpreted or limited to the actual implementation of the invention.

Please refer to FIG. 1 . FIG. 1 is a schematic diagram illustrating the relationship between devices and modules in an automated portable water pathogen detection system according to an embodiment of the present invention. The automated portable water pathogen detection system 100 in FIG. 1 includes a water inlet and outlet device 110 , a micro mixing device 120 , a detection chip 130 , a constant temperature module 140 , a control device 150 , an Internet of Things module 160 and a display module 180 .

Please refer to Fig. 1, Fig. 2 and Fig. 3 at the same time. Fig. 2 is a schematic diagram of the system interior of an automated portable water pathogen detection system according to an embodiment of the present invention. A schematic diagram of a micro-mixing device of an automated portable water pathogen detection system according to an embodiment. The water inlet and outlet device 110 includes a water pump 111 and a drainage aid 112 . The water pump 111 pumps the sample water of the water area to be tested to the third container 403 . The drainage aid 112 discharges the detected liquid to be tested from the automatic portable water pathogen detection system 100 .

As shown in FIG. 1 , FIG. 3 and FIG. 4 , FIG. 4 is a schematic diagram of a microchip of a portable water pathogen detection system according to an embodiment of the present invention. The micro-mixing device 120 includes a micro-pump 122 and a micro-chip 121 . The aforementioned micro-pumps 122 include, for example, a first micro-pump 122a, a second micro-pump 122b, a third micro-pump 122c, and a fourth micro-pump 122d, which are respectively connected to the first container 401, the The second container 402 containing the second reagent, the third container 403 containing sample water, and the sample liquid container 131 communicate with each other. Through the first micro-pump 122a, the second micro-pump 122b and the third micro-pump 122c, the first reagent, the second reagent and sample water can enter the micro-pump. The wafer 121 is mixed by using the first helical micro-mixing channel 121d and the second helical micro-mixing channel 121e to obtain the liquid to be tested. Then, the micro-pump 122d injects the test solution into the test solution container 131 . The number of reagents used may be different due to different detection items. Therefore, the number of micro-pumps and the number of containers used to accommodate various reagents can be increased or decreased according to the number of reagents used.

According to another embodiment of the present invention, the above-mentioned first reagent and second reagent may be, for example, MV-KIT (multiple vibrio detection kit) reagent and buffer respectively. The MV-KIT reagent will irreversibly react with live bacteria to generate a red reduction product, and the higher the concentration of live bacteria in the sample, the faster the coloring rate will be.

According to another embodiment of the present invention, the stocks of the first container 401 , the second container 402 and the third container 403 may be 20-40 mL, for example. According to another embodiment of the present invention, the detection of pathogenic bacteria in water is carried out once, because for example, only 20 μL of sample water, 180 μL of buffer solution and 625 μL of MV-KIT reagent are consumed. Therefore, in any two of the first container 401, the second container 402, and the third container 403, after filling the MV-KIT reagent and the buffer solution respectively, the automatic portable water pathogen detection system 100 can detect continuously for 20 days above.

According to another embodiment of the present invention, the schematic diagram of the above-mentioned microchip can be referred to FIG. 4 . The microchip 121 may include, for example, a first reagent inlet 121a, a second reagent inlet 121b, a sample water inlet 121c, a first helical micro-mixing channel 121d, a second helical micro-mixing channel 121e, a sample liquid outlet 121f, etc. device. As can be seen from Fig. 4, after the sample water (such as the above-mentioned sample water) is injected into the microchip 121 from the sample water inlet 121c, the second reagent (such as the above-mentioned buffer solution) entered by the second reagent inlet 121b is formed in the first spiral Micromixing The flow channel 121d performs mixing. Then, the previous mixed solution is mixed with the first reagent (such as the above-mentioned MV-KIT reagent) entered by the first reagent inlet 121a with the second spiral micro-mixing channel 121e to obtain the test solution, and the test solution is allowed to Various reagents and pathogenic bacteria in the medium begin to undergo a color reaction. The liquid to be tested can be transferred from the liquid to be tested outlet 121f to the liquid to be tested container 131 of the detection chip 130 with the help of the fourth micro-pump 122d (shown in FIG. 3 ). Similar to the number of micro-pumps and the number of containers for containing various reagents, the number of reagent inlets of the micro-chip and the number of spiral micro-mixing channels can be increased or decreased according to the number of reagents used.

Please refer to FIG. 1 and FIG. 5 at the same time. FIG. 5 is a schematic diagram of a detection chip of an automated portable water pathogen detection system according to an embodiment of the present invention. In FIG. 1 and FIG. 5 , the detection chip 130 mainly includes a liquid container 131 to be tested, a light emitting diode 132 as a light source, and a photodiode 133 as a light detector. In addition, in FIG. 5 , the exhaust hole 134 located above the liquid to be tested container 131 , and the water inlet hole 135 and the drain hole 136 located below the liquid to be tested container 131 are also shown. When the test liquid enters the test liquid container 131 through the water inlet 135 located below the test liquid container 131 , the gas in the test liquid container 131 can be discharged through the exhaust hole 134 located above the test liquid container 131 . After the measurement is completed, the detected liquid to be tested can be discharged from the automatic portable water pathogen detection system 100 by, for example, the drainage pump 112 (shown in FIG. 2 ).

As shown in FIG. 5 , the light emitting diode 132 and the photodiode 133 are respectively located on opposite sides of the liquid container 131 to be tested. Therefore, when performing detection, the light-emitting diode 132 first emits detection light, and the detection light passes through the liquid to be tested in the liquid to be tested container 131 , and then reaches the photodiode 133 for light detection. Since the photodiode 133 can convert the received light into a current or a voltage signal, a voltage signal can be obtained for each measurement. Moreover, the liquid to be tested will undergo a color reaction. When the liquid to be tested enters the liquid to be tested container 131 and the fluid is stable, the light-emitting diode 132 and the photodiode 133 are used to measure the color depth of the color, when V _i /V When ₀ is less than the preset value C, the concentration of pathogenic bacteria in the liquid to be tested is obtained by converting the detection time according to the pre-established detection table.

Therefore, in order to ensure that the color reaction in the liquid to be tested reaches a certain level for each sample, the initial voltage value V ₀ is first obtained when performing the first measurement. Then, the measurement is repeated at intervals, and several voltage values V _i can be obtained sequentially, where i is a positive integer. For example, a measurement is performed every 15 minutes, and the voltage values V ₁ , V ₂ , V ₃ . . . can be obtained sequentially. When V _i /V ₀ is less than a preset value C, it means that the color reaction has reached a certain level, and it is determined that the current detection has been completed. For example, the above preset value C may be 80-95%.

According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system 100 is used for detection, and the detection concentration range of water pathogens may be, for example, 10 ⁰ -10 ⁸ (CFU/mL).

As shown in FIG. 1 , the constant temperature module 140 is used to control the temperature of the liquid to be tested at a set temperature. According to another embodiment of the present invention, the constant temperature module 140 is arranged beside the detection chip 130 , and the constant temperature module 140 includes a temperature sensing chip 141 and a heating chip 142 . The temperature sensing chip 141 can sense the ambient temperature of the detection chip 130. When the ambient temperature of the detection chip 130 is lower than the set temperature, the heating sheet 142 will be heated until the ambient temperature of the detection chip 130 is higher than or equal to the set temperature. Turn off heat. The above heating chip 142 can be any available heating device.

As shown in FIG. 1 , the control device 150 is connected to the detection chip 130 , receives the initial voltage value V ₀ and the subsequent voltage value V _i from the detection chip 130 , and calculates the ratio (V _i /V ₀ ) of the two. That is, a measurement is performed every 15 minutes until V _i /V ₀ is less than the preset value C, that is, when it is judged that the color reaction has reached a certain level, it is judged that the current detection has been completed, and then the measurement is stopped, and the record is from V ₀ - The detection time spent by V _i , and the detection time is used to push back the concentration result according to the standard concentration measurement table established in advance. Table 1 is a standard concentration test table according to an embodiment of the present invention. For example, when V _i /V ₀ is less than the preset value C, and the detection time is 7 hours and 25 minutes, it is determined that the concentration of the liquid to be tested is 10 ⁶ (CFU/mL).

The control device 150 is also connected to and controls the switches of the water inlet and outlet device 110 , the micro-mixing device 120 , the detection chip 130 , the constant temperature module 140 and the sterilization module 190 .

As shown in FIG. 1 , the display module 180 is connected to the control device 150 to display water quality information. The above water quality information can be, for example, the date, time, temperature, number of tests, reagent inventory, V _i , V ₀ , V _i /V ₀ ratio of each test, and the concentration of pathogenic bacteria converted from the standard concentration test table. .

As shown in FIG. 1 , the IoT module 160 is connected to the control device 150 for uploading water quality information to the server 170 , and the pathogenic bacteria information is drawn into charts by cloud computing, massive data analysis and other technologies.

According to another embodiment of the present invention, the above water quality information can be transmitted to web pages and mobile phone applications through the server 170 . According to another embodiment of the present invention, the test data that can be seen on the above-mentioned web page can be, for example, date, time, temperature, test times, reagent stock, each V _i , V ₀ , V/V ₀ ratio, absorbance and The concentration of pathogenic bacteria converted by the standard concentration test table.

According to another embodiment of the present invention, the operation interface of the above-mentioned mobile phone application program may include the current water quality status, related information, and program termination. The above-mentioned water quality status includes temperature and pathogenic bacteria concentration. The above-mentioned relevant information may be, for example, water quality testing standards.

According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system 100 further includes a sterilization module 190 connected to and controlled by the control device 150 . The sterilization module 190 cleans and sterilizes the micro-mixing device 120 , the detection chip 130 and all pipelines of the automatic portable water pathogen detection system 100 to ensure the correctness of the detection results.

According to another embodiment of the present invention, as shown in FIG. 2 , the sterilizing module 190 further includes an ultraviolet lamp 192 , so that the automatic portable water pathogen detection system 100 can be irradiated with ultraviolet light to perform sterilization and cleaning.

According to another embodiment of the present invention, as shown in FIG. 2 , the sterilizing module 190 includes the alcohol container 191 in FIG. 2 , and its capacity may be 250 mL, for example. After the alcohol container 191 is filled with alcohol, the automatic portable water pathogen detection system 100 can be cleaned continuously for tens of days.

According to another embodiment of the present invention, the above-mentioned water inlet and outlet device 110, micro-mixing device 120, detection chip 130, constant temperature module 140, control device 150, Internet of Things module 160 and display module 180 are accommodated in an outer shell .

Please refer to FIG. 6 . FIG. 6 is a front view showing the appearance of an automatic portable water pathogen detection system according to an embodiment of the present invention. The front view of the appearance of the automatic portable water pathogen detection system 100 of the present invention includes a display module 180 for displaying water quality information and two control buttons. The above-mentioned control buttons include a MODE button 501 and a START button 502 . Use the control MODE button 501 to select one of several operating modes, such as filling reaction reagents, automatic detection mode and cleaning mode. After selecting the mode, press the START button 502 to start the selected operation mode. When no button is pressed, the automated portable water pathogen detection system 100 will remain in a power-saving standby mode. When it is necessary to automatically detect the water quality, just switch to the automatic detection function, and the system can start to automatically detect and upload data, so that users can easily monitor from the remote end.

In order to facilitate the user to sterilize and clean the automatic portable water pathogen detection system 100, a cleaning mode is added to the system. After setting with the mode switching button and pressing the start button, the automatic portable water pathogen detection system 100 is ready to use. Can automatically pre-fill 75% alcohol in the alcohol container 191 (shown in Figure 2), for automatic All possible contaminated pipelines in the portable water pathogen detection system 100 are cleaned. In the cleaning mode, for example, the micro-pump 122 and the drain pump 112 are first activated to drain the liquid in the micro-chip 121 and the liquid-to-be-tested container 131 . Next, the peristaltic pump (not shown in the figure) extracts alcohol from the alcohol container 191 to the container for storing the sample water, fills the microchip 121 and the liquid to be tested container 131 with alcohol through the micro pump 122 and then discharges the alcohol. This mode can be used when the automatic portable water pathogen detection system 100 has not been used for a long time or when the user suspects that the system pipeline is polluted. It can eliminate possible errors without the assistance of professional technicians.

According to another embodiment of the present invention, when detecting for the first time or when reagents need to be filled, use the Mode button 501 to switch to the display module 180 to display "Mode: Fill Reagents", and press the Start button 502 to make the automation portable The system for detecting pathogenic bacteria in water 100 starts to perform the action of filling reagents. The process takes about several minutes, and the reagents can be replenished by itself without the assistance of professional technicians.

According to another embodiment of the present invention, the mode of filling reagents (Mode: Fill Reagents) is set to respond to the user's first use of the automated portable water pathogen detection system 100 or the need to fill detection reagents. The micro-pump 122 used in the present invention has the effect of a stop valve when it is at rest. Therefore, when the reagent is added for the first time, the reaction reagent cannot flow into the pipeline between the micro-pump 122 and the first container 401, the second container 402, and the third container 403 due to the influence of pressure. Air can cause great errors in detection. Therefore, after the reagent is filled and before the first test, it is necessary to perform a reagent filling mode to remove the air in the pipeline to ensure the correctness of the subsequent test data. In the reagent filling mode, the micro-pumps 122 of different reagents will be activated sequentially, and the connecting tubes will be connected with the reaction reagents. The air in the road is exhausted. Then use one of the micro-pumps 122 to inject air to empty the fluid in the first helical micro-mixing channel 121d and the second helical micro-mixing channel 121e. Finally, the discharge pump 112 is activated to discharge the waste liquid in the liquid to be tested container 131 , that is, the reagent filling mode is completed.

According to another embodiment of the present invention, when the automatic detection is to be started, switch the operation mode with the Mode button 501 until the display module 180 displays "Mode: Testing", press the Start button 502, and the automatic portable water pathogenic bacteria The detection system 100 starts to perform automatic detection. Thereafter, without any manual operation, the automated portable water pathogen detection system 100 can automatically complete detection, cleaning, uploading data, etc., and automatically detects every day. If the reaction reagent is full, the automatic portable water pathogen detection system 100 can automatically detect tens of days. According to another embodiment of the present invention, the automated portable water pathogen detection system 100 can automatically record the number of detection times and reagent inventory, and remind the application program whether the reagent inventory is sufficient to perform the required number of detection times and view the results.

Please refer to FIG. 7 . FIG. 7 is a schematic flowchart illustrating a method for using an automated portable water pathogen detection system according to an embodiment of the present invention. Please also refer to Figure 1-7.

In step 201 , the water inlet and outlet device 110 draws sample water from the water area to be tested to the third container 403 . According to another embodiment of the present invention, before the official test, the sample water in the third container 403 is first extracted by the micro-pump 122 to fill the liquid to be tested container 131, and then drained from the drain hole 136 of the liquid to be tested container 131. To discharge, repeat this action 1-2 times to discharge the remaining impurities.

In step 202, the constant temperature module 140 controls the temperature of the liquid to be tested to maintain the liquid to be tested at a set temperature. According to another embodiment of the present invention, the thermostat module 140 maintains the liquid to be tested at the set temperature in the following way: the thermostat module 140 will determine whether the ambient temperature of the detection chip 130 is lower than the set temperature, when the environment of the detection chip 130 When the temperature is lower than the set temperature, heating is started, and the heating is stopped until the ambient temperature of the detection chip 130 is not lower than the set temperature.

In step 203, the micro-mixing device 120 sequentially fills the sample water, the buffer solution and the MV-KIT reagent into the micro-chip 121 and mixes them uniformly to perform a color reaction. For example, sample water, buffer solution and MV-KIT reagent are uniformly mixed in the first helical micro-mixing channel 121d and the second helical micro-mixing channel 121e respectively in the amounts of 0.02mL, 0.18mL and 0.625mL. , the color reaction is started, and the mixed test solution is sent to the test solution container 131 of the detection wafer 130 .

In step 204, the detection chip 130 performs the first measurement of the liquid to be tested, and obtains the voltage (average) value obtained from the first measurement as an initial voltage value (V ₀ ). In step 205, after waiting for a period of time, let the detection wafer 130 measure the liquid to be tested again to obtain the subsequent voltage value (V _i ). The above-mentioned initial voltage value (V ₀ ) and subsequent voltage value (V _i ) may also be average values obtained after several consecutive measurements.

In step 206, it is judged whether the color reaction has reached a certain level. For example, the control device 150 judges whether the ratio of the _subsequent voltage value (V i ) to the initial voltage value (V ₀ ) is less than the preset value C according to the acquired initial voltage value (V ₀ ) and subsequent voltage value (V _i ), and also That is, whether V _i /V ₀ <C. If V _i /V ₀ <C, it means that the color reaction has reached a certain level, and the detection is completed, and then enter step 207 . If V _i /V ₀ ≧C, it means that the color reaction has not reached a certain level, it is necessary to repeat step 205 again after a period of time, until V _i /V ₀ <C, the detection is ended, and step 207 is entered. Using the relative voltage ratio as the criterion for judging whether the detection is completed can eliminate the influence of the original color and turbidity of the liquid to be tested on the detection result.

In step 207, the concentration of the liquid to be tested is converted by the measurement time according to the standard concentration calibration table to generate water quality information. The above water quality information includes the date, time, temperature of each test, each V _i , V ₀ , V _i /V ₀ ratio, and the concentration of pathogenic bacteria converted from the standard concentration check table in Table 1. According to another embodiment of the present invention, step 207 further includes uploading the water quality information to the server 170 by using the IoT module 160 . According to yet another embodiment of the present invention, the above water quality information can also be uploaded to a cloud database. The above-mentioned cloud database can be, for example, the Thingspeaks cloud database.

In step 208 , the display module 180 displays the water quality information transmitted from the control device 150 .

In step 209 , the detected liquid to be tested is discharged from the automated portable water pathogen detection system 100 through the drainage pump 112 .

In step 210, after the liquid to be tested is discharged from the automated portable water pathogen detection system 100, the sterilization module 190 automatically performs a cleaning process. The sterilization module 190 cleans and sterilizes all the pipelines of the micro-mixing device 120 and the detection chip 130 of the automatic portable water pathogen detection system 100 . For example, the first reagent inlet 121a, the second reagent inlet 121b, the sample water inlet 121c, the first helical micro-mixing channel 121d, the second helical micro-mixing channel 121e, the test liquid outlet 121f and the test liquid container 131, etc., to ensure the correctness of the test results.

設定天數(30天)時，進入步驟220中，自動化可攜式水中病原菌檢測系統100將停止自動檢測模式。 In step 212, the automated portable water pathogen detection system 100 enters a standby state after cleaning is completed. In step 214, wait until a preset time is reached from the start time of the last detection, for example, 24 hours. That is, when the start time of the last detection reaches 24 hours, enter step 216, and add 1 day to the number of detection days D. For example, when testing on the first day, the number of testing days is D=1, and after the preset time (24 hours), the number of testing days is D=2. When the number of detection days D is less than a set number of days, for example, the set number of days is 30 days. When D<30 days, the automatic portable water pathogen detection system 100 will automatically detect again, and repeat steps 201-218 until the number of detection days D

When the number of days is set (30 days), enter step 220, and the automatic portable water pathogen detection system 100 will stop the automatic detection mode.

According to another embodiment of the present invention, after the automatic detection mode is stopped, after the first container 401, the second container 402, and the third container 403 are filled with reagents, and after the completion of the filling reaction reagent mode, the automatic portable water pathogenic bacteria The detection system 100 resets the set number of days of detection to restart the automatic detection mode, and repeats steps 201-220.

According to another embodiment of the present invention, if the automatic portable water pathogen detection system 100 is terminated due to uncontrollable factors during the detection process, such as power failure, abnormal detection data or long-term non-use, the "cleaning mode" can be used first. ”The action of manually sterilizing and cleaning the automated portable water pathogen detection system 100. Before performing manual sterilization and cleaning, it is necessary to confirm whether the alcohol stock in the alcohol container 191 is sufficient. If it is enough, use the Mode button 501 to switch the operation mode until the display module 180 displays "Mode: Clean", then press the Start button 502 to make the automatic portable water pathogen detection system 100 start cleaning. The time of above-mentioned cleaning process is tens of minutes, for example can be 10-50 minute. This mode can be used when the system has not been used for a long time or when the user suspects that the system pipeline is contaminated. Therefore, possible errors can be eliminated without the assistance of professional technicians.

In summary, the present invention provides an automated portable water pathogen detection system and method, which is a fast, automatic, real-time detection system and method with the function of the Internet of Things, without the need for complex optical instruments and excessive time and manpower , has a certain degree of accuracy, and can be continuously detected for dozens of days. Only a very small amount of sample water is required to be mixed through the micro-mixing device, and the color change of the sample is converted into the corresponding voltage value (V) and the detection result value is generated. In addition, the system of the present invention can upload the measured data to the network, for example, the detected data can be uploaded to the network by wireless transmission method, and then the data can be captured through the application program. Therefore, even if people are not next to the detection system, they can directly see the real-time detection data through mobile devices such as computers and mobile phones.

Although the embodiments of the present invention have been disclosed above, it is not intended to limit the present invention. Those who are familiar with the art can make various changes within the spirit and scope of the present invention without departing from the spirit and scope of the present invention. and retouching, so the protection scope of the present invention should be defined by the scope of the patent application later.

100:Automated portable water pathogen detection system

110: Water inlet and outlet device

120: micro mixing device

121:Microchip

122:Micro pump

130: Detection wafer

131: container of liquid to be tested

132: light emitting diode

133: photodiode

140: Constant temperature module

141: temperature sensing chip

142: heating sheet

150: Control device

160:Internet of things module

170: server

180: display module

190: Sterilization module

Claims (10)

An automatic portable water pathogen detection system, which includes: a water inlet and outlet device, which draws a sample water from a water area to be tested and discharges a tested liquid after detection; a micro mixing device, connected to the water inlet and outlet device, which includes A plurality of micropumps and a microchip having at least one helical micro-mixing flow channel, through which the sample water and a plurality of reaction reagents are sequentially mixed in the at least one helical micro-mixing flow channel to obtain The liquid to be tested; a detection chip, connected to the micro-mixing device, which includes: a container for the liquid to be tested, used to accommodate the liquid to be tested; a light emitting diode; and a photodiode, which detects the light from the The light intensity of the diode and output the corresponding voltage value, wherein the light-emitting diode and the photodiode are respectively located on the opposite sides of the liquid container to be tested; a sterilizing module for the micro-mixing device and the detection The chip is cleaned and sterilized; a control device is connected to and controls the water inlet and outlet device, the micro-mixing device and the detection chip, and receives the voltage value; and a display module is connected with the control device to display a water quality information . The automatic portable water pathogen detection system as described in claim 1, wherein the sterilizing module further includes an ultraviolet lamp. The automatic portable pathogen detection system in water as described in claim 1 further includes a constant temperature module for controlling the temperature of the liquid to be tested at a set temperature. The automatic portable water pathogen detection system as described in claim 3, wherein the constant temperature module includes: A temperature sensing chip, which senses the ambient temperature of the detection chip; and a heating chip, which heats the environment of the detection chip to the set temperature. The automatic portable pathogenic bacteria detection system in water as described in claim 1, wherein the detection chip also includes: an exhaust hole, located above the liquid container to be tested; and a water inlet hole and a drain hole, both located Below the liquid container. The automatic portable water pathogen detection system as described in Claim 1, wherein the microchip further includes a plurality of reagent inlets, which communicate with the container of the sample water and the containers of the reaction reagents respectively. The automatic portable pathogenic bacteria detection system in water as described in Claim 1, wherein the micro-pumps communicate with the sample water container, the reaction reagent containers and the test liquid container respectively. The automated portable water pathogen detection system as described in Claim 1 further includes an Internet of Things module for uploading the water quality information to a server. A method for using the automatic portable water pathogen detection system as described in claim 1, comprising: (A) the water inlet and outlet device pumps the sample water from the water area to be tested; (B) the micro-mixing device draws the sample water Mix with the reaction reagents in the spiral micro-mixing flow channel to obtain the test solution and perform a color reaction; (C) the detection chip measures the test solution to obtain a corresponding voltage value (V _i ) , wherein the voltage value obtained from the first detection is an initial voltage value (V ₀ ); (D) the control device judges according to the voltage value (V _i ) of the liquid to be tested and the initial voltage value (V ₀ ) Whether V _i /V ₀ is less than a preset value; (E) when V _i /V ₀ is less than the preset value, generate the water quality information according to the measurement time; (F) discharge the tested liquid after detection; ( G) Clean the micro-mixing device and the pipeline of the detection chip; (H) When the last detection time reaches a preset time, repeat the above steps (A)-(G) until the number of days of detection is greater than or equal to a setting When the number of days, the detection is stopped. The method for using the automatic portable water pathogen detection system as described in claim 9, wherein the detection chip performs the first measurement of the liquid to be tested, obtains the initial voltage value (V ₀ ), and after a fixed time interval, The detection wafer is measured again to obtain the voltage value (V _i ) obtained from the subsequent measurement.

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