TWI795735B - Automatic portable detection system for pathogenic bacteria - Google Patents
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本發明是有關於一種水中病原菌檢測系統,特別是一種自動化可攜式水中病原菌檢測系統。 The invention relates to a detection system for pathogenic bacteria in water, in particular to an automatic portable detection system for pathogenic bacteria in water.
目前常見的水質檢測儀器主要可區分為實驗室分析法與現場即時分析法。前者例如使用聚合酶連鎖反應(Polymerase chain reaction,PCR)或是快篩系統,因檢測儀器體積龐大且昂貴而無法搬運,且須透過人員收集樣品水回實驗室分析,故受限於地點而不具便利性,雖其檢測設備性能高而可獲得較佳的靈敏度及偵測極限,卻無法進行現場即時性檢測。後者使用的儀器,雖市面上已有各種檢測儀器,但無法自動化取樣、檢測及記錄。有鑑於此,對於開發可移動式、自動化檢測、快速且即時的可攜式水中病原菌檢測系統為最有效的方式,可大幅提升台灣水產養殖業的生產力與競爭力。 At present, the common water quality testing instruments can be mainly divided into laboratory analysis method and on-site instant analysis method. The former uses polymerase chain reaction (Polymerase chain reaction, PCR) or rapid screening system, which cannot be transported due to the bulky and expensive detection equipment, and the samples must be collected by personnel and returned to the laboratory for analysis, so it is limited by the location and does not have Convenience, although its detection equipment has high performance and can obtain better sensitivity and detection limit, it cannot perform on-site real-time detection. The latter uses instruments, although there are various testing instruments on the market, but they cannot automate sampling, testing and recording. In view of this, it is the most effective way to develop a mobile, automatic detection, fast and real-time portable water pathogen detection system, which can greatly improve the productivity and competitiveness of Taiwan's aquaculture industry.
鑑於上述欲解決之問題及其原因,具體而言,本發明提供一種自動化可攜式水中病原菌檢測系統,可連續檢測數十天。僅需要極少量的樣品水透過微型混合裝置進行混合,並根據樣品之呈色變化轉換為對應之電壓值。當呈色反應到達一定程度時,產生檢測結果值。並且本發明之系統可將所測得之數據上傳到伺服器,供即時取得測驗結果。 In view of the above-mentioned problems to be solved and their reasons, specifically, the present invention provides an automatic and portable detection system for pathogenic bacteria in water, which can continuously detect dozens of days. Only a very small amount of sample water is required to be mixed through the micro-mixing device, and converted into a corresponding voltage value according to the color change of the sample. When the color reaction reaches a certain level, the detection result value is generated. And the system of the present invention can upload the measured data to the server for real-time acquisition of test results.
本發明為一種自動化可攜式水中病原菌檢測系統,其包括進出水裝置、微型混合裝置、檢測晶片、控制裝置以及顯示模組。上述進出水裝置抽取待檢測水域之樣品水及排出檢測後之待測液。上述微型混合裝置,連接上述進出水裝置,其包括多個微型幫浦及具有至少一螺旋形微混合流道之微型晶片,透過多個微型幫浦依序將樣品水與多個反應試劑在至少一螺旋形微混合流道中混合,得到待測液。上述檢測晶片連接上述微型混合裝置,其包括待測液容器、發光二極體以及光電二極體。上述待測液容器用以容納待測液。上述光電二極體偵測來自發光二極體的光線強度並輸出對應的電壓值,其中發光二極體和光電二極體分別位於待測液容器的相對兩側。上述控制裝置,連接並控制上述進出水裝置、微型混合裝置以及檢測晶片,接收電壓值。上述顯示模組,與上述控制裝置連接,用以顯示水質資訊。 The invention is an automatic portable water pathogen detection system, which includes a water inlet and outlet device, a micro mixing device, a detection chip, a control device and a display module. The above-mentioned water inlet and outlet device draws sample water from the water area to be tested and discharges the tested liquid after testing. The above-mentioned micro-mixing device is connected to the above-mentioned water inlet and outlet device, which includes a plurality of micro-pumps and a micro-chip with at least one spiral micro-mixing flow channel, through which the sample water and a plurality of reaction reagents are sequentially mixed at least Mix in a spiral micro-mixing flow channel to obtain the test solution. The detection chip is connected to the micro-mixing device, which includes a liquid container to be tested, a light emitting diode and a photodiode. The liquid to be tested container is used for containing the liquid to be tested. The photodiode detects the light intensity from the light emitting diode and outputs a corresponding voltage value, wherein the light emitting diode and the photodiode are respectively located on opposite sides of the liquid container to be tested. The above-mentioned control device is connected to and controls the above-mentioned water inlet and outlet device, the micro-mixing device and the detection chip, and receives the voltage value. The above-mentioned display module is connected with the above-mentioned control device for displaying water quality information.
根據本發明之另一實施例,上述自動化可攜式水中病原菌檢測系統更包括殺菌模組,其與控制裝置連接。殺菌模組針對上述微型混合裝置以及檢測晶片進行清洗及殺菌,確保檢測結果的正確性。 According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes a sterilizing module connected to the control device. The sterilization module cleans and sterilizes the above-mentioned micro-mixing device and the detection chip to ensure the correctness of the detection results.
根據本發明之另一實施例,上述自動化可攜式水中病原菌檢測系統更包括恆溫模組,與控制裝置連接。恆溫模組控制待測液的溫度在一設定溫度。 According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes a constant temperature module connected to the control device. The constant temperature module controls the temperature of the liquid to be tested at a set temperature.
根據本發明之另一實施例,上述恆溫模組包括溫度感測晶片及加熱片。上述溫度感測晶片感測檢測晶片之環境溫度,上述加熱片用來維持檢測晶片於設定的溫度上。亦即,當環境溫度低於設定溫度時,上述加熱片會進行持續加熱,當檢測晶片之環境溫度高於等於設定溫度,則停止加熱。 According to another embodiment of the present invention, the above constant temperature module includes a temperature sensing chip and a heating chip. The temperature sensing chip senses the ambient temperature of the detection chip, and the heating plate is used to maintain the detection chip at a set temperature. That is, when the ambient temperature is lower than the set temperature, the above-mentioned heating chip will continue to heat, and when the ambient temperature of the detected chip is higher than or equal to the set temperature, the heating will be stopped.
根據本發明之另一實施例,上述檢測晶片還包括排氣孔、入水孔和排水孔。上述排氣孔,位於待測液容器之上方。上述入水孔和排水孔,皆位於待測液容器之下方。 According to another embodiment of the present invention, the detection wafer further includes exhaust holes, water inlet holes and drain holes. The vent hole is located above the container of the liquid to be tested. The above-mentioned water inlet hole and drain hole are all located below the container of the liquid to be tested.
根據本發明之另一實施例,上述微型晶片更包括多個試劑入口,分別與樣品水之容器與反應試劑之容器相通。 According to another embodiment of the present invention, the above-mentioned microchip further includes a plurality of reagent inlets, respectively communicating with the sample water container and the reaction reagent container.
根據本發明之另一實施例,上述多個微型幫浦分別與樣品水之容器、反應試劑之容器以及待測液容器相通。 According to another embodiment of the present invention, the plurality of micro-pumps communicate with the sample water container, the reaction reagent container and the test liquid container respectively.
根據本發明之另一實施例,上述自動化可攜式水中病原菌檢測系統更包括物聯網模組,用以上傳水質資訊至伺服器。 According to another embodiment of the present invention, the above-mentioned automated portable water pathogen detection system further includes an Internet of Things module for uploading water quality information to the server.
根據本發明之另一實施例,上述水質資訊可透過伺服器傳遞至網頁及手機應用程式。 According to another embodiment of the present invention, the above water quality information can be transmitted to web pages and mobile phone applications through the server.
本發明為一種自動化可攜式水中病原菌檢測系統之使用方法,其包括:(A)進出水裝置抽取待檢測水域之樣品水。(B)微型混合裝置將樣品水與反應試劑以螺旋形微混合流道混合,取得待測 液並進行呈色反應。(C)檢測晶片對待測液進行測量以取得對應之電壓值(Vi),其中第一次檢測所得之電壓值為初始電壓值(V0)。(D)控制裝置根據該電壓值(Vi)與該初始電壓值(V0)判斷Vi/V0是否小於一預設值。(E)當Vi/V0小於預設值時,依據測量時間產生水質資訊。(F)將檢測後的待測液排出。(G)清洗微型混合裝置以及檢測晶片之管路。(H)當距離上次檢測時間達到一預設時間時,重複上述步驟(A)-(G),直至檢測之天數大於等於一設定天數時,才停止檢測。 The present invention is a method for using an automatic portable pathogenic bacteria detection system in water, which includes: (A) a water inlet and outlet device to extract sample water from a water area to be tested. (B) The micro-mixing device mixes the sample water and the reaction reagent in a spiral micro-mixing channel to obtain the liquid to be tested and perform a color reaction. (C) The detection chip measures the liquid to be tested to obtain the corresponding voltage value (V i ), wherein the voltage value obtained from the first detection is the initial voltage value (V 0 ). (D) The control device judges whether V i /V 0 is smaller than a preset value according to the voltage value (V i ) and the initial voltage value (V 0 ). (E) When V i /V 0 is less than a preset value, water quality information is generated according to the measurement time. (F) Discharging the detected liquid to be tested. (G) Clean the micro-mixing device and the pipeline for detecting chips. (H) When the time from the last detection reaches a preset time, repeat the above steps (A)-(G), until the number of days of detection is greater than or equal to a set number of days, then stop the detection.
根據本發明之另一實施例,上述檢測晶片對待測液進行第一次測量,取得初始電壓值(V0),隔一固定時間後,檢測晶片再次進行測量,得到後續測量所得之電壓值(Vi)。 According to another embodiment of the present invention, the detection chip performs the first measurement of the liquid to be tested to obtain the initial voltage value (V 0 ), and after a fixed period of time, the detection chip performs measurement again to obtain the voltage value ( V i ).
綜上所述,本發明提供之一種自動化可攜式水中病原菌檢測系統,為一快速、自動化、即時且具物聯網功能之檢測系統,不需複雜的光學儀器及過多時間和人力,並且擁有一定程度的準確度,可利用無線傳輸方法將檢測所得數據上傳到網路,透過應用程式抓取資料,即使人不在檢測系統旁邊,也能透過電腦、手機等行動裝置直接看到即時的檢測數據。 To sum up, the present invention provides an automated portable detection system for pathogenic bacteria in water, which is a fast, automatic, real-time detection system with the function of the Internet of Things. It does not require complicated optical instruments and excessive time and manpower, and has a certain The degree of accuracy can be uploaded to the network through wireless transmission methods, and the data can be captured through the application program. Even if people are not next to the detection system, they can directly see the real-time detection data through mobile devices such as computers and mobile phones.
另外,由於台灣水中養殖魚塭的分布較為零散,自動化移動式檢測系統便不受地區的限制,能讓使用者輕易的自行進行水質的檢測與監控。 In addition, because the distribution of aquaculture fish farms in Taiwan is relatively scattered, the automated mobile detection system is not restricted by region, allowing users to easily conduct water quality detection and monitoring by themselves.
100:自動化可攜式水中病原菌檢測系統 100:Automated portable water pathogen detection system
110:進出水裝置 110: Water inlet and outlet device
111:抽水幫浦 111: water pump
112:排水幫補 112: Drainage help
120:微型混合裝置 120: micro mixing device
121:微型晶片 121:Microchip
121a:第一試劑入口 121a: first reagent inlet
121b:第二試劑入口 121b: Second reagent inlet
121c:樣品水入口 121c: sample water inlet
121d:第一螺旋形微混合流道 121d: the first spiral micro-mixing channel
121e:第二螺旋形微混合流道 121e: the second spiral micro-mixing channel
121f:待測液排出口 121f: outlet for the liquid to be tested
122:微型幫浦 122:Micro pump
122a:第一微型幫浦 122a: The first micro pump
122b:第二微型幫浦 122b: The second micro-pump
122c:第三微型幫浦 122c: The third micro-pump
122d:第四微型幫浦 122d: The fourth micro-pump
130:檢測晶片 130: Detection wafer
131:待測液容器 131: container of liquid to be tested
132:發光二極體 132: light emitting diode
133:光電二極體 133: photodiode
134:排氣孔 134: exhaust hole
135:入水孔 135: water inlet hole
136:排水孔 136: drainage hole
140:恆溫模組 140: Constant temperature module
141:溫度感測晶片 141: temperature sensing chip
142:加熱片 142: heating sheet
150:控制裝置 150: Control device
160:物聯網模組 160:Internet of things module
170:伺服器 170: server
180:顯示模組 180: display module
190:殺菌模組 190: Sterilization module
191:酒精容器 191: Alcohol container
192:紫外燈 192:UV lamp
193:紫外燈控制器 193:UV lamp controller
201-220:步驟 201-220: Steps
401:第一容器 401: first container
402:第二容器 402: second container
403:第三容器 403: The third container
為了讓本發明之上述和其他目的、特徵、優點與實施例能更明顯易懂,所附附圖之說明如下: In order to make the above and other objects, features, advantages and embodiments of the present invention more comprehensible, the accompanying drawings are described as follows:
圖1係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統中各裝置、模組的關係架構圖。 FIG. 1 is a schematic diagram showing the relationship between devices and modules in an automated portable water pathogen detection system according to an embodiment of the present invention.
圖2係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之系統內部的示意圖。 FIG. 2 is a schematic diagram illustrating the interior of an automated portable water pathogen detection system according to an embodiment of the present invention.
圖3係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之微型混合裝置的示意圖。 FIG. 3 is a schematic diagram of a micro-mixing device of an automated portable water pathogen detection system according to an embodiment of the present invention.
圖4係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之微型晶片的示意圖。 FIG. 4 is a schematic diagram of a microchip of an automated portable water pathogen detection system according to an embodiment of the present invention.
圖5係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之檢測晶片的示意圖。 FIG. 5 is a schematic diagram of a detection chip of an automated portable water pathogen detection system according to an embodiment of the present invention.
圖6係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之外觀前視圖。 FIG. 6 is a front view showing the appearance of an automated portable water pathogen detection system according to an embodiment of the present invention.
圖7係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之使用方法的流程示意圖。 FIG. 7 is a schematic flowchart illustrating a method for using an automated portable water pathogen detection system according to an embodiment of the present invention.
為利瞭解本發明之特徵、內容與優點,茲將本發明配合圖式,並以實施例之表達形式詳細說明如下,而其中所使用之圖式,其主旨僅為示意及輔助說明書之用,未必為本發明實施後之真實比例 與精準配置,故不應就所附之圖式的比例與配置關係解讀、侷限本發明於實際實施上的申請專利範圍。 In order to facilitate the understanding of the features, content and advantages of the present invention, the present invention is hereby combined with the drawings and described in detail in the form of embodiments as follows, and the drawings used therein are only for illustration and auxiliary description purposes. Not necessarily the true ratio after the implementation of the present invention Therefore, the proportion and configuration relationship of the attached drawings should not be interpreted or limited to the actual implementation of the invention.
請參閱圖1,圖1係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統中各裝置、模組的關係架構圖。圖1的自動化可攜式水中病原菌檢測系統100包括進出水裝置110、微型混合裝置120、檢測晶片130、恆溫模組140、控制裝置150、物聯網模組160以及顯示模組180。
Please refer to FIG. 1 . FIG. 1 is a schematic diagram illustrating the relationship between devices and modules in an automated portable water pathogen detection system according to an embodiment of the present invention. The automated portable water
請同時參考圖1、圖2及圖3,圖2係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之系統內部的示意圖,圖3係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之微型混合裝置的示意圖。進出水裝置110包括抽水幫浦111以及排水幫補112。抽水幫浦111抽取待檢測水域之樣品水至第三容器403。排水幫補112將檢測後之待測液從自動化可攜式水中病原菌檢測系統100內排出。
Please refer to Fig. 1, Fig. 2 and Fig. 3 at the same time. Fig. 2 is a schematic diagram of the system interior of an automated portable water pathogen detection system according to an embodiment of the present invention. A schematic diagram of a micro-mixing device of an automated portable water pathogen detection system according to an embodiment. The water inlet and
如圖1、圖3及圖4所示,圖4係繪示依據本發明之一實施例之一種可攜式水中病原菌檢測系統之微型晶片的示意圖。微型混合裝置120包括微型幫浦122及微型晶片121。上述微型幫浦122例如包括第一微型幫浦122a、第二微型幫浦122b、第三微型幫浦122c以及第四微型幫浦122d,其分別與用來容納第一試劑的第一容器401、容納第二試劑的第二容器402、容納樣品水的第三容器403以及待測液容器131相通。透過上述第一微型幫浦122a、第二微型幫浦122b及第三微型幫浦122c,讓第一試劑、第二試劑和樣品水可以進入微型
晶片121,利用第一螺旋形微混合流道121d和第二螺旋形微混合流道121e進行混合,得到待測液。然後,微型幫浦122d將待測液注入待測液容器131中。由於需要檢測項目不同而可能使得所用試劑種類的數量不同,因此上述微型幫浦的數量以及用來容納各種試劑的容器數量,可因應所用試劑種類多寡的需求而增減其數量。
As shown in FIG. 1 , FIG. 3 and FIG. 4 , FIG. 4 is a schematic diagram of a microchip of a portable water pathogen detection system according to an embodiment of the present invention. The
根據本發明之另一實施例,上述的第一試劑、第二試劑例如可分別為MV-KIT(multiple vibrio detecting kit)試劑與緩衝液。MV-KIT試劑會與活菌體進行不可逆反應,生成紅色的還原產物,且樣品中活菌濃度越高,呈色速率越快。 According to another embodiment of the present invention, the above-mentioned first reagent and second reagent may be, for example, MV-KIT (multiple vibrio detection kit) reagent and buffer respectively. The MV-KIT reagent will irreversibly react with live bacteria to generate a red reduction product, and the higher the concentration of live bacteria in the sample, the faster the coloring rate will be.
根據本發明之另一實施例,第一容器401、第二容器402以及第三容器403之存量例如可為20-40mL。根據本發明之另一實施例,實施檢測水中病原菌一次,由於例如僅需耗用20μL的樣品水、180μL的緩衝液以及625μL的MV-KIT試劑。因此,於第一容器401、第二容器402以及第三容器403之任兩者之中,分別填滿MV-KIT試劑及緩衝液後,自動化可攜式水中病原菌檢測系統100可連續檢測20天以上。
According to another embodiment of the present invention, the stocks of the
根據本發明之另一實施例,上述微型晶片之示意圖可參閱圖4。微型晶片121例如可包括第一試劑入口121a、第二試劑入口121b、樣品水入口121c、第一螺旋形微混合流道121d、第二螺旋形微混合流道121e、待測液排出口121f等裝置。由圖4可知,先讓樣品水(如上述之樣品水)由樣品水入口121c注入微型晶片121後,與由第二試劑入口121b進入的第二試劑(如上述之緩衝液)以第一螺旋形微混合
流道121d進行混合。接著,讓前面混合液再與由第一試劑入口121a進入的第一試劑(如上述之MV-KIT試劑)以第二螺旋形微混合流道121e進行混合,得到待測液,讓待測液中各種試劑與病原菌開始進行呈色反應。待測液可藉由第四微型幫浦122d(示於圖3)之助,自待測液排出口121f將待測液傳送至檢測晶片130的待測液容器131之中。類似於上述微型幫浦的數量以及用來容納各種試劑的容器數量,上述微型晶片的試劑入口的數量以及螺旋形微混合流道的數量,可因應所用試劑種類多寡的需求而增減其數量。
According to another embodiment of the present invention, the schematic diagram of the above-mentioned microchip can be referred to FIG. 4 . The
請同時參考圖1和圖5,圖5係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之檢測晶片的示意圖。在圖1和圖5中,檢測晶片130主要包括待測液容器131、作為光源的發光二極體132以及作為光偵測器的光電二極體133。此外,在圖5中,還顯示位於待測液容器131上方之排氣孔134,以及位於待測液容器131下方之入水孔135和排水孔136。當待測液通過位於待測液容器131下方之入水孔135進入待測液容器131之同時,待測液容器131內的氣體可透過位於待測液容器131上方之排氣孔134排除之。待測量完成後,例如可藉由排水幫浦112(示於圖2)將檢測後的待測液從自動化可攜式水中病原菌檢測系統100內排出。
Please refer to FIG. 1 and FIG. 5 at the same time. FIG. 5 is a schematic diagram of a detection chip of an automated portable water pathogen detection system according to an embodiment of the present invention. In FIG. 1 and FIG. 5 , the
如圖5所示,發光二極體132和光電二極體133分別位於待測液容器131的相對兩側。因此在進行檢測時,發光二極體132先發出檢測光線,讓檢測光線通過待測液容器131內的待測液之後,再到達光電二極體133進行光線偵測。由於光電二極體133能將接收
的光線轉換成電流或者電壓訊號,所以每次測量皆可得到一電壓訊號。而且,待測液會進行呈色反應,當待測液進入待測液容器131且流體穩定時,利用發光二極體132和光電二極體133測量呈色的顏色深度,當Vi/V0小於預設值C時,以檢測時間依據事先建立好的檢量表來換算得知待測液中之病原菌的濃度。
As shown in FIG. 5 , the
所以,為了確定每個樣品的待測液中之呈色反應到達一定程度,在進行第一次測量時,先得初始電壓值V0。然後,每隔一段時間重複進行一次測量,可依序得到數個電壓值Vi,i為正整數。例如每隔15分鐘進行一次測量,可依序得到電壓值V1、V2、V3...等。等到Vi/V0小於一預設值C時,可代表呈色反應到達一定程度,判定當次檢測已完成。例如上述預設值C例如可為80-95%。 Therefore, in order to ensure that the color reaction in the liquid to be tested reaches a certain level for each sample, the initial voltage value V 0 is first obtained when performing the first measurement. Then, the measurement is repeated at intervals, and several voltage values V i can be obtained sequentially, where i is a positive integer. For example, a measurement is performed every 15 minutes, and the voltage values V 1 , V 2 , V 3 . . . can be obtained sequentially. When V i /V 0 is less than a preset value C, it means that the color reaction has reached a certain level, and it is determined that the current detection has been completed. For example, the above preset value C may be 80-95%.
根據本發明之另一實施例,使用上述自動化可攜式水中病原菌檢測系統100來進行檢測,水中病原菌的檢測濃度區間例如可為100-108(CFU/mL)。
According to another embodiment of the present invention, the above-mentioned automated portable water
如圖1所示,恆溫模組140係用來控制待測液的溫度在一設定溫度上。根據本發明之另一實施例,上述恆溫模組140配置於檢測晶片130旁,恆溫模組140包括溫度感測晶片141及加熱片142。溫度感測晶片141會感測檢測晶片130之環境溫度,當檢測晶片130之環境溫度低於設定溫度時,加熱片142會進行加熱,直至檢測晶片130之環境溫度高於等於設定溫度時,才停止加熱。上述加熱片142可為任一可用之加熱裝置。
As shown in FIG. 1 , the
如圖1所示,控制裝置150與檢測晶片130連接,接收檢測晶片130傳來的初始電壓值V0和後續電壓值Vi,計算出兩者的比值(Vi/V0)。亦即每15分鐘進行一次測量,直至Vi/V0小於預設值C時,亦即判斷呈色反應已到達一定程度時,判定當次檢測已完成,才停止測量,記錄從V0-Vi所花的檢測時間,並以此檢測時間依據事先建立好的標準濃度檢量表回推濃度結果。表1為根據本發明之一實施例之標準濃度檢量表。例如,當Vi/V0達到小於預設值C時,所耗費的檢測時間為7小時25分鐘,則判斷待測液之濃度為106(CFU/mL)。
As shown in FIG. 1 , the
上述控制裝置150還連接並控制進出水裝置110、微型混合裝置120、檢測晶片130、恆溫模組140以及殺菌模組190之開關。
The
如圖1所示,顯示模組180與上述控制裝置150連接用以顯示水質資訊。上述水質資訊例如可為每次檢測時的日期、時間、
溫度、檢測次數、試劑存量、每次的Vi、V0、Vi/V0比值以及經標準濃度檢量表換算後之病原菌濃度。
As shown in FIG. 1 , the
如圖1所示,物聯網模組160與控制裝置150連接,用以上傳水質資訊至伺服器170,藉由雲端運算、巨量資料分析等技術將病原菌資訊繪製成圖表。
As shown in FIG. 1 , the
根據本發明之另一實施例,上述水質資訊可透過伺服器170傳遞至網頁及手機應用程式。根據本發明之另一實施例,上述網頁中可看到的檢測數據例如可為日期、時間、溫度、檢測次數、試劑存量、每次的Vi、V0、V/V0比值、吸光度以及經標準濃度檢量表換算後之病原菌濃度。
According to another embodiment of the present invention, the above water quality information can be transmitted to web pages and mobile phone applications through the
根據本發明之另一實施例,上述手機應用程式之操作介面可包括水質現況、相關資訊以及結束程式。上述水質現況包括溫度以及病原菌濃度。上述相關資訊例如可為水質檢測標準。 According to another embodiment of the present invention, the operation interface of the above-mentioned mobile phone application program may include the current water quality status, related information, and program termination. The above-mentioned water quality status includes temperature and pathogenic bacteria concentration. The above-mentioned relevant information may be, for example, water quality testing standards.
根據本發明之另一實施例,上述自動化可攜式水中病原菌檢測系統100更包括殺菌模組190,與控制裝置150連接並由控制裝置150控制。殺菌模組190會針對自動化可攜式水中病原菌檢測系統100之微型混合裝置120、檢測晶片130以及所有管路進行清洗及殺菌,確保檢測結果的正確性。
According to another embodiment of the present invention, the above-mentioned automated portable water
根據本發明之另一實施例,如圖2所示,殺菌模組190更包括紫外燈192,因此可利用紫外光照射上述自動化可攜式水中病原菌檢測系統100進行殺菌清理的動作。
According to another embodiment of the present invention, as shown in FIG. 2 , the sterilizing
根據本發明之另一實施例,如圖2所示,殺菌模組190包括圖2中的酒精容器191,其容量例如可為250mL。在酒精容器191中填滿酒精後,可連續清洗自動化可攜式水中病原菌檢測系統100達數十天。
According to another embodiment of the present invention, as shown in FIG. 2 , the sterilizing
根據本發明之另一實施例,上述進出水裝置110、微型混合裝置120、檢測晶片130、恆溫模組140、控制裝置150、物聯網模組160及顯示模組180係收容在一外殼體中。
According to another embodiment of the present invention, the above-mentioned water inlet and
請參閱圖6,圖6係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之外觀的前視圖。本發明之自動化可攜式水中病原菌檢測系統100的外觀前視圖上包括顯示水質資訊之顯示模組180以及兩顆控制按鈕。上述控制按鈕包括MODE按鈕501以及START按鈕502。利用控制MODE按鈕501可在幾種操作模式中選擇其中一種操作模式,上述操作模式例如可為填充反應試劑、自動檢測模式以及清洗模式。選擇好模式之後按下START按鈕502便可開始進行所選操作模式。在無任何按鈕被按下時,自動化可攜式水中病原菌檢測系統100將維持在省電的待機模式。當需自動檢測水質時,則只要切換成自動檢測功能,即可使系統開始自動進行檢測與上傳數據,讓使用者可輕鬆從遠端監控。
Please refer to FIG. 6 . FIG. 6 is a front view showing the appearance of an automatic portable water pathogen detection system according to an embodiment of the present invention. The front view of the appearance of the automatic portable water
為了方便使用者自行為自動化可攜式水中病原菌檢測系統100進行滅菌清洗的動作,故系統增設一清洗模式,以模式切換按鈕進行設定後按下啟動鍵,自動化可攜式水中病原菌檢測系統100即會自動以預先填入75%酒精於酒精容器191(示於圖2)中,對自動化
可攜式水中病原菌檢測系統100中所有可能受到汙染的管路進行清洗。清洗模式例如,先啟動微型幫浦122與排水幫浦112將微型晶片121與待測液容器131之液體排出。接著,蠕動幫浦(未示於圖中)從酒精容器191內抽取酒精至存放樣品水之容器,透過微型幫浦122將酒精填滿微型晶片121與待測液容器131後再將酒精排出。本模式可用於自動化可攜式水中病原菌檢測系統100長期未使用或是使用者懷疑系統管路受到汙染時進行,無須藉由專業技術人員協助,即可自行排除可能誤差。
In order to facilitate the user to sterilize and clean the automatic portable water
根據本發明之另一實施例,當第一次檢測或是需要填充試劑時,以Mode按鈕501切換至顯示模組180顯示「Mode:Fill Reagents」,並按下Start按鈕502,使自動化可攜式水中病原菌檢測系統100開始執行填充試劑的動作,過程約數分鐘,無須藉由專業技術人員協助,即可自行完成試劑補充。
According to another embodiment of the present invention, when detecting for the first time or when reagents need to be filled, use the
根據本發明之另一實施例,填充試劑模式(Mode:Fill Reagents)的設置,是為了因應使用者第一次使用自動化可攜式水中病原菌檢測系統100或是有需要填充檢測試劑時。本發明使用之微型幫浦122在靜止時有止流閥的功效。故在第一次添加試劑時,會因為壓力的影響使反應試劑無法流入微型幫浦122與第一容器401、第二容器402、第三容器403之間的管路,此段管路中的空氣會使檢測造成極大的誤差。故在填充試劑後且進行第一次檢測前,需先執行一次試劑填充模式以排除管路中空氣,確保後續檢測數據之正確性。試劑填充模式會先依序啟動不同試劑之微型幫浦122,以反應試劑將連接管
路內之空氣排出。再以微型幫浦122其中之一打入空氣,將第一螺旋形微混合流道121d和第二螺旋形微混合流道121e內之流體排空。最後,啟動排出幫浦112將待測液容器131內之廢液排出,即完成試劑填充模式。
According to another embodiment of the present invention, the mode of filling reagents (Mode: Fill Reagents) is set to respond to the user's first use of the automated portable water
根據本發明之另一實施例,當要開始自動檢測時,以Mode按鈕501切換操作模式,直至顯示模組180顯示「Mode:Testing」時,按下Start按鈕502,使自動化可攜式水中病原菌檢測系統100開始執行自動檢測的動作。此後,無須任何人工操作,自動化可攜式水中病原菌檢測系統100即可自動完成檢測、清洗、上傳數據等工作,並每天自動進行檢測。若反應試劑填滿的情況下,自動化可攜式水中病原菌檢測系統100可自動檢測數十天。根據本發明之另一實施例,自動化可攜式水中病原菌檢測系統100可自動記錄檢測次數與試劑存量,並於應用程式提醒試劑存量是否充足以執行所需檢測次數及觀看結果。
According to another embodiment of the present invention, when the automatic detection is to be started, switch the operation mode with the
請參閱圖7,圖7係繪示依據本發明之一實施例之一種自動化可攜式水中病原菌檢測系統之使用方法的流程示意圖。請同時參閱圖1-7。 Please refer to FIG. 7 . FIG. 7 is a schematic flowchart illustrating a method for using an automated portable water pathogen detection system according to an embodiment of the present invention. Please also refer to Figure 1-7.
在步驟201中,進出水裝置110抽取待檢測水域之樣品水至第三容器403。根據本發明之另一實施例,在正式檢測之前,先以微型幫浦122抽取第三容器403內之樣品水填滿待測液容器131,再從待測液容器131之排水孔136將之排出,重複此動作1-2次,將先前殘留的雜質排出。
In
在步驟202中,恆溫模組140控制待測液的溫度,讓待測液維持在設定溫度。根據本發明之另一實施例,恆溫模組140使待測液維持在設定溫度上之方式為,恆溫模組140會判斷檢測晶片130之環境溫度是否低於設定溫度,當檢測晶片130之環境溫度低於設定溫度時,則開始加熱,直至檢測晶片130之環境溫度未低於設定溫度時,才停止加熱。
In
在步驟203中,微型混合裝置120將樣品水、緩衝液以及MV-KIT試劑依序填入微型晶片121中均勻混合,進行呈色反應。例如,樣品水、緩衝液以及MV-KIT試劑以0.02mL、0.18mL、0.625mL的量依序分別以第一螺旋形微混合流道121d和第二螺旋形微混合流道121e中,均勻混合,開始進行呈色反應,並將混合完成的待測液送至檢測晶片130的待測液容器131之中。
In
在步驟204中,檢測晶片130對待測液進行第一次測量,取得第一次測量所得之電壓(平均)值為初始電壓值(V0)。在步驟205中,等一段時間後,讓檢測晶片130再次對待測液進行測量,得到後續電壓值(Vi)。上述之初始電壓值(V0)和後續電壓值(Vi),亦可為連續數次測量後所取的平均值。
In
在步驟206中,要判斷呈色反應是否到達一定程度。例如,控制裝置150依據取得之初始電壓值(V0)和後續電壓值(Vi),判斷後續電壓值(Vi)與初始電壓值(V0)之比值是否小於預設值C,亦即是否Vi/V0<C。若Vi/V0<C,表示呈色反應已到達一定程度,檢測完成,則進入步驟207中。若Vi/V0≧C,表示呈色反應還未到達一定程
度,則需隔一段時間後再次重複步驟205,直至Vi/V0<C為止,結束檢測,進入步驟207。上述藉由相對電壓比值做為檢測是否完成的判斷標準,可去除待測液原本顏色與濁度對檢測結果的影響。
In
在步驟207中,以測量時間依據標準濃度檢量表換算待測液濃度,產生水質資訊。上述水質資訊包括每一次測驗之日期、時間、溫度、每次的Vi、V0、Vi/V0比值以及經表1之標準濃度檢量表換算後之病原菌濃度。根據本發明之另一實施例,步驟207更包括可使用物聯網模組160上傳水質資訊至伺服器170。根據本發明之再一實施例,上述水質資訊還可上傳至雲端資料庫。上述雲端資料庫例如可為Thingspeaks雲端資料庫。
In
在步驟208中,顯示模組180顯示控制裝置150所傳來的水質資訊。
In
在步驟209中,透過排水幫浦112將檢測後之待測液從自動化可攜式水中病原菌檢測系統100排出。
In
在步驟210中,待檢測後之待測液排出自動化可攜式水中病原菌檢測系統100後,殺菌模組190自動進行一次清洗流程。殺菌模組190針對自動化可攜式水中病原菌檢測系統100之微型混合裝置120和檢測晶片130之所有管路進行清洗及殺菌。例如,第一試劑入口121a、第二試劑入口121b、樣品水入口121c、第一螺旋形微混合流道121d、第二螺旋形微混合流道121e、待測液排出口121f以及待測液容器131等,以確保檢測結果的正確性。
In
在步驟212中,待清洗完成後自動化可攜式水中病原菌檢測系統100會進入待機狀態。在步驟214中,等到距離上次檢測的開始時間達到一預設時間的時候,例如為24小時。亦即,當距離上次檢測的開始時間達到24小時後,進入步驟216中,將檢測天數D加1天。例如,第一天檢測時,檢測天數D=1,過了預設時間(24小時)之後,檢測天數D=2。當檢測天數D小於一設定天數時,例如,設定天數為30天。當D<30天時,自動化可攜式水中病原菌檢測系統100會再次自動檢測,重複步驟201-218,直至當檢測天數D設定天數(30天)時,進入步驟220中,自動化可攜式水中病原菌檢測系統100將停止自動檢測模式。
In
根據本發明之另一實施例,停止自動檢測模式後,待第一容器401、第二容器402以及第三容器403填充試劑完成,並待填充反應試劑模式執行完成後,自動化可攜式水中病原菌檢測系統100重置檢測之設定天數,即可重啟自動檢測模式,重複執行步驟201-220。
According to another embodiment of the present invention, after the automatic detection mode is stopped, after the
根據本發明之另一實施例,若自動化可攜式水中病原菌檢測系統100於檢測過程中因不可控因素而終止,例如停電、檢測數據不正常或是長期未使用時,可先使用「清洗模式」對自動化可攜式水中病原菌檢測系統100進行手動殺菌清理的動作。在進行手動殺菌清理之前,需先確認酒精容器191內之酒精存量是否足夠。若足夠,則以Mode按鈕501切換操作模式,直至顯示模組180顯示「Mode:Clean」時,按下Start按鈕502,使自動化可攜式水中病原菌檢測系統100開始清洗的動作。上述清洗過程之時間為數十分鐘,例如可為10-50
分鐘。本模式可用於系統長期未使用時或是使用者懷疑系統管路受到汙染時,因此無須藉由專業技術人員協助,即可自行排除可能誤差。
According to another embodiment of the present invention, if the automatic portable water
綜上所述,本發明提供之一種自動化可攜式水中病原菌檢測系統及方法,為一快速、自動化、即時且具物聯網功能之檢測系統及方法,不需複雜的光學儀器及過多時間和人力,擁有一定程度的準確度,並且可連續檢測數十天。僅需要極少量的樣品水透過微型混合裝置進行混合,並根據樣品之呈色變化轉換為對應之電壓值(V)以及產生檢測結果值。另外,本發明之系統可將所測得之數據上傳到網路,例如可利用無線傳輸方法將檢測所得數據上傳到網路,再透過應用程式抓取資料。所以,即使人不在檢測系統旁邊,也能透過電腦、手機等行動裝置直接看到即時的檢測數據。 In summary, the present invention provides an automated portable water pathogen detection system and method, which is a fast, automatic, real-time detection system and method with the function of the Internet of Things, without the need for complex optical instruments and excessive time and manpower , has a certain degree of accuracy, and can be continuously detected for dozens of days. Only a very small amount of sample water is required to be mixed through the micro-mixing device, and the color change of the sample is converted into the corresponding voltage value (V) and the detection result value is generated. In addition, the system of the present invention can upload the measured data to the network, for example, the detected data can be uploaded to the network by wireless transmission method, and then the data can be captured through the application program. Therefore, even if people are not next to the detection system, they can directly see the real-time detection data through mobile devices such as computers and mobile phones.
雖然本發明已實施方式揭露如上,然其並非用以限定本發明,凡熟悉該項技藝之人士其所依本發明之精神,在不脫離本發明之精神和範圍內,當可作各種之更動與潤飾,因此本發明之保護範圍當視後之申請專利範圍所界定者為準。 Although the embodiments of the present invention have been disclosed above, it is not intended to limit the present invention. Those who are familiar with the art can make various changes within the spirit and scope of the present invention without departing from the spirit and scope of the present invention. and retouching, so the protection scope of the present invention should be defined by the scope of the patent application later.
100:自動化可攜式水中病原菌檢測系統 100:Automated portable water pathogen detection system
110:進出水裝置 110: Water inlet and outlet device
120:微型混合裝置 120: micro mixing device
121:微型晶片 121:Microchip
122:微型幫浦 122:Micro pump
130:檢測晶片 130: Detection wafer
131:待測液容器 131: container of liquid to be tested
132:發光二極體 132: light emitting diode
133:光電二極體 133: photodiode
140:恆溫模組 140: Constant temperature module
141:溫度感測晶片 141: temperature sensing chip
142:加熱片 142: heating sheet
150:控制裝置 150: Control device
160:物聯網模組 160:Internet of things module
170:伺服器 170: server
180:顯示模組 180: display module
190:殺菌模組 190: Sterilization module
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TW200517659A (en) * | 2003-09-24 | 2005-06-01 | Intel Corp | Programmable molecular barcodes |
TW201211242A (en) * | 2010-06-17 | 2012-03-16 | Geneasys Pty Ltd | Microfluidic device for genetic and mitochondrial analysis of a biological sample |
TW202028744A (en) * | 2019-01-18 | 2020-08-01 | 國立清華大學 | Automatic microfluidic system for rapid personalized drug screening and testing method for personalized antibiotic susceptibility |
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TW200517659A (en) * | 2003-09-24 | 2005-06-01 | Intel Corp | Programmable molecular barcodes |
TW201211242A (en) * | 2010-06-17 | 2012-03-16 | Geneasys Pty Ltd | Microfluidic device for genetic and mitochondrial analysis of a biological sample |
TW202028744A (en) * | 2019-01-18 | 2020-08-01 | 國立清華大學 | Automatic microfluidic system for rapid personalized drug screening and testing method for personalized antibiotic susceptibility |
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