TWI774331B - Fermented product of michelia alba and oat, method of manufacturing the same and topical composition including the same - Google Patents

Abstract

The invention provides a fermented product of Michelia albaand oat, a method of manufacturing the same and a topical composition including the same. In the method, a ferment substrate including the flower of Michelia albaand oat bran is subjected to a fermenting step, thereby obtaining the fermented product of Michelia albaand oat. The aforementioned fermented product of Michelia albaand oat has an excellent antioxidant capacity, an ability to reduce the symptom of broken capillaries of skin, and safety. Thus, the fermented product of Michelia albaand oat can be applied in a topical composition.

Description

Magnolia oat ferment, its production method and composition for external use containing the same

The present invention relates to a magnolia flower oat ferment, in particular to a magnolia oat ferment that can effectively improve the symptoms of redness of the skin, a manufacturing method thereof and a composition containing the same for external use.

In addition to providing a place for the exchange of nutrients and metabolites, microvessels can also regulate body temperature through dilation. When the capillaries dilate, the blood flow under the skin increases, causing the skin to become reddened. However, if the stratum corneum of the skin is thinned and/or the elasticity of the microvessels is fatigued, the skin is prone to redness symptoms, including persistent redness and/or visible microvessels under the skin, and even accompanied by fever, itching and rashes, etc. symptom.

Congenitally sensitive skin, excessive exposure to ultraviolet rays, improper maintenance (such as: excessive cleansing, excessive exfoliation, excessive skin resurfacing and/or excessive laser treatment), abnormal life and rest, environmental factors (such as: high temperature, low temperature or Factors such as sandstorms) and/or the use of drugs (such as steroids) may cause redness on the skin. Use skin-repairing skin care products to improve red blood However, if you choose improper skin care products, it will lead to skin allergies, resulting in more serious red blood symptoms.

Magnolia ( Michelia alba ) is a plant of the genus Michelia of the family Magnoliaceae, and has other aliases such as white orchid, white magnolia and fragrant flower. Magnolia is a common garden plant. It can be used as medicine to relieve cough, reduce phlegm and diuresis. In addition, the extracts of magnolia flowers (such as essential oils and gels) can be used in aromatherapy. However, magnolia flower extraction is not easy, and its extract has problems such as poor skin feel. Secondly, there is no research to confirm whether magnolia flower extract has the effect of improving redness symptoms.

Therefore, there is an urgent need for a magnolia flower oat fermented product, its manufacture method and its composition for external use, to solve the above-mentioned problems.

Therefore, one aspect of the present invention is to provide a manufacturing method of a magnolia flower oat leaven. The method uses yeast to ferment a fermented substrate comprising magnolia flower, oat bran and water to obtain a magnolia flower oat fermented product.

Another aspect of the present invention is to provide a magnolia flower oat fermented product, wherein the magnolia flower oat fermented product is prepared by the above manufacturing method, and the magnolia flower oat fermented product comprises protein, crude polysaccharides (crude polysaccharides), total flavonoids and total phenols.

Another aspect of the present invention is to provide an external composition for improving skin redness symptoms, wherein the external use composition contains the magnolia flower oat ferment as an effective ingredient for improving skin redness symptoms.

According to the above aspect of the present invention, there is provided a method for producing a magnolia flower oat leaven. First, a fermented base is provided, wherein the fermented base may include, but is not limited to, magnolia ( Michelia alba ) flowers, oat bran, and water. Next, using yeast ( Saccharomyces cerevisiae ) to perform a fermentation step on the fermentation substrate at 20°C to 50°C for 10 hours to 50 hours, thereby obtaining a magnolia flower oat fermentation product.

According to an embodiment of the present invention, the above-mentioned manufacturing method may further include at least drying and grinding the magnolia flower and oat bran to obtain mixed powder, wherein the temperature of the drying step may be, for example, not more than 80°C.

According to an embodiment of the present invention, the weight-to-volume ratio of the mixed powder and the yeast liquid can be, for example, 2-20:5-10 (g:mL). In one embodiment, the weight-to-volume ratio of magnolia flower, oat bran and water may be, for example, 2-10:2-10:100-300 (g:g:mL). In one embodiment, the bacterial concentration of the above-mentioned yeast can be, for example, 10 ⁵ CFU/mL to 10 ⁸ CFU/mL.

According to an embodiment of the present invention, the yeast can be deposited in Taiwan Bioresource Collection and Research Center (BCRC), for example, and the deposit number is BCRC 21815.

According to an embodiment of the present invention, the above-mentioned manufacturing method may further include a solid-liquid separation step and/or a sterilization step after the fermentation step.

According to the above aspect of the present invention, there is provided a magnolia flower oat leaven. The magnolia flower oat fermented product is prepared by the above method, and each milliliter of the magnolia flower oatmeal fermented product may contain 1.0 mg to 5.0 mg of protein, 5.0 mg to 15.0 mg of crude polysaccharides, 1.0 mg to 1.8 mg of total flavonoids, and 0.1 mg to 0.5 mg of total phenols. In addition, the pH of the magnolia oat starter can be, for example, 4.5 to 6.8.

According to the above aspect of the present invention, there is provided a composition for external use for improving redness of the skin. The aforementioned composition for external use may include the aforementioned magnolia flower oat leaven as an effective ingredient for improving the symptoms of redness of the skin.

Applying the magnolia flower oat fermented product of the present invention, its manufacturing method and the external composition containing the same for improving skin redness symptoms, a specific yeast ferment can be used to prepare a fermented substrate comprising magnolia flower, oat bran and water. have to. The magnolia flower oat ferment has the efficacy and safety of anti-oxidation, improving skin redness symptoms, and can be used as an effective substance of a topical composition for improving skin redness symptoms.

In order to make the above and other objects, features, advantages and embodiments of the present invention more clearly understood, the detailed description of the accompanying drawings is as follows:

[Fig. 1] is a line graph of the concentration of the magnolia oat fermented liquid versus the scavenging percentage of DPPH free radicals according to an embodiment.

[ Fig. 2A ] to [ Fig. 2C ] are photos showing the skin of the affected part of some subjects without using the magnolia oat fermented liquid according to an embodiment.

[ Fig. 3A ] to [ Fig. 3C ] are photos showing the skin of the affected part of the subjects shown in Fig. 2A to Fig. 2C , respectively, after using the magnolia flower oat fermented liquid for 14 days.

[Fig. 4] is a graph showing the severity of redness symptoms of the affected part skin according to an embodiment of whether the magnolia flower oat fermented liquid is used or not.

Based on the above, the present invention provides a magnolia oat fermented product. The above magnolia oat fermented product is obtained by using yeast to perform a fermenting step on a specific fermented substrate. The magnolia flower oat ferment has the efficacy and safety of anti-oxidation and improving the symptoms of redness of the skin, and can be used as an active ingredient of an external composition for improving the symptoms of redness of the skin.

Specifically, the above-mentioned fermented base contains magnolia flower and oat bran. Magnolia flower extracts are often used in aromatherapy to cleanse the skin, inhibit skin fungus and/or firm the skin. However, the extraction efficiency of magnolia flower is low, and the magnolia flower extract has problems such as poor skin feel. The "skin feel" mentioned herein refers to the feel of the skin to the external composition, wherein the viscosity of the external composition affects the user's subjective perception of the skin feel of the external composition.

The above-mentioned oat bran refers to the epidermis of oat ( Avena sativa ), wherein oat is an annual herb of the genus Avena graminis. Oat bran is rich in water-soluble beta-glucan, vitamin B1, vitamin B2, vitamin E, minerals and dietary fiber, and has anti-wrinkle, anti-aging, beauty and beauty effects and good transdermal absorption. Therefore, oat bran is widely used in anti-wrinkle and anti-aging skin care products.

In one embodiment, the above magnolia flower, oat bran and water are mixed to obtain a fermented base. Next, using yeast ( Saccharomyces cerevisiae ) to perform a fermentation step on the fermentation substrate at 20° C. to 50° C. for 10 hours to 50 hours, thereby obtaining a magnolia flower oat fermentation product. If the fermentation step is performed at a temperature lower than 20° C., the activity of the yeast is not good, resulting in poor fermentation efficiency. If the fermentation step is carried out at a temperature higher than 50°C, the yeast may lose activity or even die. If the time of the fermenting step is less than 10 hours, the content of the active ingredient of the fermented product is insufficient. On the contrary, if the time of the fermenting step exceeds 50 hours, there may be concerns about the generation of miscellaneous bacteria. In one embodiment, the fermentation step is performed at 30°C for about 24 hours. In one embodiment, the weight-to-volume ratio of magnolia flower, oat bran and water can be, for example, 2~10:2~10:100~300 (g:g:mL), so that the magnolia flower oat fermented product has better Antioxidant and improve red blood capillary effect.

In one embodiment, before the fermentation step, the above-mentioned magnolia flower and oat bran may be subjected to a drying step and a grinding step to obtain a mixed powder, wherein the magnolia flower includes petals and stamens, and optionally includes pedicels. The above drying step can be performed by conventional methods, such as freeze drying, vacuum drying and spray drying. In one embodiment, magnolia flowers may be dried, for example, at a temperature not exceeding 80°C. In one embodiment, magnolia flowers may be dried, for example, at a temperature of 70°C to 80°C. The above-mentioned grinding step can be performed by conventional methods, such as: mechanical grinding, drum grinding and flowing gas grinding. In one embodiment, the particle size of the mixed powder may be, for example, 30 meshes to 80 meshes. In a preferred embodiment, the particle size of the mixed powder can be, for example, 80 meshes, so as to facilitate subsequent operations (such as solid-liquid separation), provide a better specific surface area for reaction with yeast, and make magnolia oatmeal. The fermented product has better viscosity and better skin feel.

In one embodiment, before the fermentation step, the yeast may be pre-treated, wherein the pre-treatment may include performing a first liquid culture, a solid state culture and a second liquid culture at 20°C to 50°C. The above-mentioned first liquid culture and second liquid culture can be carried out using a conventional culture solution, and a specific example may be a potato dextrose broth (PDB). The above-mentioned solid-state culture can be performed using a conventional medium, and a specific example can be potato dextrose agar (PDA). After the first liquid culture, the activity of the yeast is improved, and after the solid state culture, a single strain can be obtained by screening a single colony, thereby avoiding the growth of miscellaneous bacteria. After the second liquid culture, a bacterial liquid with an optic density (OD) of 0.5 to 1.0 can be obtained, wherein the growth cycle of the yeast is in the logarithmic phase at this time, the yeast has better activity, and the bacterial liquid is in the logarithmic phase. The bacterial concentration of the yeast is 10 ⁵ CFU/mL to 10 ⁸ CFU/mL.

In one embodiment, the above-mentioned yeasts are deposited in the Food Industry Research and Development Institute (FIRDI) Bioresource Collection and Research Center (BCRC) (331 Food Road, Hsinchu, Taiwan). number) and the deposit number is BCRC 21815. In one embodiment, the weight-to-volume ratio of the mixed powder and the yeast liquid is 2-20:5-10 (g:mL) to achieve better fermentation efficiency. In one embodiment, the fermentation step is performed on 7 g of the mixed powder with 5 mL of yeast. The fermented substrate and yeast can be adjusted according to the above ratio according to the needs.

In one embodiment, after the fermenting step, a solid-liquid separation step and/or a sterilization step are performed on the magnolia oat fermented product. The above-mentioned solid-liquid separation step can be carried out, for example, by conventional methods, such as filtration treatment, centrifugation treatment, distillation treatment, etc., to remove the solid content of the magnolia oat fermented product. In one embodiment, the centrifugation is performed at 4000 rpm to 12000 rpm for 15 to 30 minutes. In one embodiment, centrifugation is performed at 4500 rpm for 20 minutes. In one embodiment, the sterilization step can be performed by, for example, conventional methods, such as autoclaving, dry heat treatment, and ultraviolet light treatment. In a specific example, the sterilization step is performed using high pressure (1.0kg/cm ² to 1.5kg/cm ² ) steam at high temperature (110°C to 130°C) for 10 minutes to 20 minutes.

The magnolia flower oat fermented product obtained by the above-mentioned manufacturing method is a light brown translucent liquid, wherein the magnolia flower oat fermented product is 100 weight percent (wt %) based on the magnolia flower oat fermented product, and the magnolia flower oat fermented product contains 0.9wt% to 2.0wt% of Soluble solids. In one embodiment, each milliliter of magnolia oat ferment contains 1.0 mg to 5.0 mg of protein, 5.0 mg to 15.0 mg of crude polysaccharides, 1.0 mg to 1.8 mg of total flavonoids, and 0.1 mg to 0.5 mg of total flavonoids. of total phenols. In one embodiment, the pH value of the magnolia flower oat fermented product may be, for example, 4.5 to 6.8, which is close to the pH value of the skin surface, so that the bacteria phase of the normal flora on the skin surface will not be destroyed. In one embodiment, the viscosity of the magnolia flower oatmeal ferment can be, for example, 10 cP to 15 cP, which can improve the problem of poor skin feel of the magnolia flower extract.

It is worth noting that the above manufacturing steps exclude the use of organic solvents for the extraction step, so no organic solvents remain. Furthermore, the use of the above-mentioned specific strains can shorten the time of the fermentation step, and can avoid the problem of contamination by miscellaneous bacteria caused by long-term fermentation.

It has been confirmed by skin experiments that the above magnolia oat ferment has anti-oxidative properties. It can be used as an active ingredient in external compositions because of its efficacy and safety in reducing and improving skin redness symptoms. The aforementioned "antioxidant" refers to the ability to scavenge free radicals. The aforementioned "redness symptoms" include persistent redness of the skin and/or obvious microvessels, etc., which can be assessed by, for example, the hemoglobin concentration. The above "improve the symptoms of redness of the skin" means that the application of Magnolia Oatmeal Enzyme can soothe and remove redness, which means that the degree of redness of the skin is reduced and/or the microvessels are less obvious. The above "safety" means non-irritating, which means that after the Magnolia Oat Enzyme is applied to the skin, there will be no erythema, infiltration, edema, papules and herpes reactions.

In one embodiment, the dosage form of the above-mentioned composition for external use can be, for example, liquid, emulsion, cream, Anxin, capsule, pill, lozenge, powder, granule, or gel. In one embodiment, the above-mentioned external composition may be, for example, a lotion, a lotion, an essence, a barrier cream, a facial cleanser, a facial mask, or a sunscreen lotion.

Several embodiments are used below to illustrate the application of the present invention, but they are not intended to limit the present invention. Those with ordinary knowledge in the technical field of the present invention can make various changes and modifications without departing from the spirit and scope of the present invention. retouch.

Embodiment one, prepare magnolia oat fermented liquid

First, yeast (BCRC 21815) was cultured in a first liquid state with a PDB culture solution at 37°C to obtain a first bacterial solution. Next, the first bacterial solution was spread on the PDA medium to obtain a single colony of yeast. Then, the above single colony is cultured in a second liquid state with a PDB culture solution at 37° C. to obtain a second bacterial solution, wherein the OD value of the second bacterial solution is 0.5 to 1.0, which is equivalent to the second bacterial solution containing 10 ⁵ to 10 ⁸ CFU/mL of yeast cells.

Magnolia flower (including petals, stamens and pedicels) and oat bran were dried at 75℃±5℃, and then ground into 80-mesh mixed powder. Next, 7 g of the mixed powder and 300 mL of water were mixed into a fermentation substrate, and 5 mL of the second bacterial solution was added, and the fermentation step was performed at 30° C. for 24 hours to obtain a magnolia flower oat fermentation product. Next, the magnolia oat fermented product was sterilized at 121° C. with a vapor pressure of 1.1 kg/cm ² for 15 minutes, and then centrifuged at a centrifugal speed of 4500 rpm for 20 minutes to obtain a magnolia oat fermented liquid.

The above magnolia flower oat fermented liquid is a light brown translucent liquid. The viscosity and pH value of the above-mentioned magnolia oat fermented liquid are measured by conventional methods (such as: using a rotational viscometer and a pH meter), wherein the viscosity of the magnolia oat fermented liquid is 10cP to 15cP, and the pH value is 4.5 to 6.8. It is supplemented that the above-mentioned magnolia oat fermented liquid has no pathogenic bacteria detected, so it has biological safety.

After measurement, based on the above-mentioned magnolia oat fermented liquid of 100 wt %, the magnolia oat fermented liquid contains 0.9 wt % to 2.0 wt % of soluble solids. Secondly, each milliliter of magnolia oat fermented liquid contains 1.0 mg to 5.0 mg of protein, 5 mg to 15 mg of crude polysaccharide, 1.0 mg to 1.8 mg of total flavonoids and 0.1 mg to 0.5 mg of total phenols. The aforementioned total flavonoids and total phenols may contain compounds with free radical scavenging effect, so it can be inferred that the magnolia oat fermented liquid has antioxidant capacity.

Embodiment two, evaluate the antioxidant capacity of magnolia oat fermented liquid

1,1-Diphenyl-2-trinitrophenylhydrazine (1,1- diphenyl-2-picrylhydrazyl, DPPH) is a stable free radical, and the DPPH ethanol solution is blue-purple, and has a high absorbance value for light with a wavelength of 517 nm, but the DPPH ethanol solution is light yellow after being acted by antioxidants. Therefore, by measuring the change of the absorbance at 517 nm of the DPPH ethanol solution after adding a certain sample, the DPPH radical scavenging rate of this sample can be calculated, thereby evaluating the antioxidant capacity of the sample.

According to Table 1, Example 1 and Comparative Examples 1 to 3 were prepared and mixed uniformly. After standing for 5 minutes at room temperature, the absorbance at 517 nm was measured, wherein the concentration of the above DPPH ethanol solution was 1.52×10 ^-4 M, and the sample was Magnolia oat fermented liquid of Example 1 with concentrations of 10wt%, 15wt%, 20wt%, 25wt% and 30wt%.

It is worth noting that during the measurement, the above-mentioned Example 1 used the absorbance value of Comparative Example 1 as a blank background value (blank), and Comparative Example 2 used the absorbance value of Comparative Example 3 as a blank background value, so as to obtain standardization. after the absorbance. Utilize formula (I) to calculate the DPPH free radical scavenging rate of different concentrations of Magnolia oat fermented liquid.

X%=[1-(AU)/(AU ₀ )]×100% Formula (I)

Among them, X% represents the clearance percentage of DPPH, and AU and AU ₁ are the normalized absorbance values of Experimental Example 1 and Comparative Example 3, respectively.

FIG. 1 is a line graph of the concentration of magnolia oat fermented liquid versus the scavenging percentage of DPPH free radicals according to an embodiment, wherein the horizontal axis represents the concentration, and the vertical axis represents the scavenging percentage of DPPH free radicals. As shown in Figure 1, the magnolia oat fermented liquid has the ability to scavenge DPPH free radicals, and the magnolia oat fermented liquid scavenging DPPH free radicals is a dose-dependent reaction, and when the concentration of the magnolia oat fermented liquid is 30wt%, The DPPH free radical scavenging percentage was 44.8%. After calculation, when the concentration of magnolia oat fermented liquid was 32.944wt%, the scavenging percentage of DPPH free radical was 50% (n=3).

Embodiment 3. Evaluate the effect of magnolia oat fermented liquid to improve skin redness symptoms

Before using the magnolia oatmeal fermented liquid, the hemoglobin of the affected part of the subject's face was measured using a commercially available skin optical detection instrument (brand name: Antera 3D, manufacturer: Miravex, Dublin, Ireland). The concentration of erythrocytes is expressed as the erythrocyte value, wherein the erythrocyte value has no unit, and the erythrocyte value of the skin of the non-affected part of the face is defined as 1.0, so as to evaluate the degree of erythema. The larger the value of the red blood cell value, the more severe the red blood cell symptoms. Next, after using 2.0 mg of magnolia oat fermented liquid per square centimeter on the skin of the affected part for 14 days, the red blood value of the skin of the same affected part was detected by the above-mentioned optical instrument again.

2A to 2C are photos showing the skin of the affected part of some subjects without using the magnolia oat fermented liquid according to an embodiment, and FIGS. 3A to 3C 2A to 2C respectively show the photos of the skin of the affected area after using the magnolia oat fermented liquid for 14 days. Compare Figure 2A (before use, the red blood value was 1.292) and Figure 3A (after use, the red blood value was 1.117), Figure 2B (before use, the red blood value was 1.021) and Figure 3B (after use, the red blood value was 1.021) 0.9391), Figure 2C (before use, the red blood value was 1.158) and Figure 3C (after use, the red blood value was 1.046), it can be found that the symptoms of red blood were significantly improved after using the magnolia flower oat fermented liquid for 14 days.

4 is a graph showing the severity of redness symptoms of the affected skin according to an embodiment of whether or not the magnolia oat fermented liquid is used or not, wherein the horizontal axis represents no use or after 14 days of use, and the vertical axis represents the redness value. As shown in Figure 4, after using the magnolia oat fermented liquid for 14 days, the redness value of the affected skin decreased significantly by 13.31% ( p < 0.05), indicating that the magnolia oat fermented liquid can effectively improve the symptoms of redness.

Embodiment four, assess the safety of magnolia oat fermented liquid

A closed patch test was used to evaluate whether the magnolia oatmeal fermented liquid was irritating to the subject's skin. The above-mentioned subjects were randomly selected persons between the ages of 18 and 60 (30 persons in total).

First, use non-irritating tape to fix 2 plaques on the back of each subject for 24 hours, so that the filter paper in the fin-chamber of the plaque is attached to the subject's back. On the back skin, the plaque tester was not removed during the period, and the plaque tester was also prevented from touching water. The filter paper contained 0.20mL to 0.25mL of magnolia oat fermented liquid as the experimental group, and the filter paper contained 0.20mL to 0.25mL. mL of distilled water was used as the control group. After 24 hours, the plaque tester was removed, and the condition of the skin was observed after 30 minutes, 24 hours and 48 hours, respectively.

The results are recorded in Table 1, where the figure number "-" indicates that the skin is negative, "±" indicates that the skin has a suspicious reaction (also known as mild erythema: with mild erythema), and "+" indicates that the skin has a weak positive reaction ( Also known as erythematous reaction: erythema, infiltration, edema and/or papules), "++" indicates a strong positive skin reaction (also known as herpes reaction: erythema, infiltration, edema, papules, herpes, and herpes reaction skin beyond the test area), "+++" indicates a very strong positive skin reaction (also known as confluent herpes reaction: marked erythema, severe infiltration, edema, confluent herpes, and confluent herpes reaction skin beyond the test area) (n=30).

As shown in Table 2, after 30 minutes, 24 hours or 48 hours, the subjects in the control group or the experimental group had no erythema, infiltration, edema, papules, herpes and confluent blisters in and around the test area. rash, etc. Show Magnolia oatmeal fermented liquid is safe for skin.

To sum up, it is confirmed from the above-mentioned several examples that the magnolia oat fermented product of the present invention has both the efficacy and safety of anti-oxidation and improvement of red blood symptoms, so it can be used as an active ingredient of an external composition.

As can be seen from the above-described embodiments, the magnolia flower oat fermented product of the present invention and its manufacturing method, and the skin care composition containing it have the advantage of using yeast to carry out a fermenting step on a fermented substrate comprising magnolia flower and oat bran, so as to obtain Magnolia Oatmeal Starter. The composition containing the above magnolia oat ferment has the efficacy and safety of anti-oxidation, improving red blood symptoms. Secondly, the magnolia oatmeal ferment is weakly acidic, with moderate viscosity, and has a better skin feel.

It should be added that although the present invention takes a specific manufacturing process, a specific bacterial strain, a specific analytical method or a specific instrument as an example to illustrate the magnolia oat fermented product of the present invention, its manufacturing method and the resistance containing its external composition. The efficacy and safety of oxidizing and improving redness symptoms, but any person with ordinary knowledge in the technical field to which the present invention belongs will know that the present invention is not limited to this. The fermented product, its manufacturing method and its external-use composition can also be carried out using other processes, other strains, other analytical methods or other instruments.

Although the present invention has been disclosed above with several embodiments, it is not intended to limit the present invention. Anyone with ordinary knowledge in the technical field to which the present invention belongs, without departing from the spirit and scope of the present invention, can make various Therefore, the scope of protection of the present invention should be determined by the scope of the appended patent application.

Claims (10)

A manufacturing method of magnolia flower oat fermented product, comprising: providing a fermented base, wherein the fermented base comprises a mixed powder of magnolia ( Michelia alba ) flower and oat bran and water, and the magnolia flower, the oat bran And one weight-volume ratio of this water is 2～10:2～10:100～300 (g:g:mL); And utilize yeast ( Saccharomyces cerevisiae ) to carry out a fermentation step at 30 ℃ to this fermentation substrate Up to 24 hours, thereby obtaining this magnolia oat fermented product, wherein this yeast is deposited in Taiwan Bioresource Collection and Research Center (Bioresource Collection and Research Center, BCRC), deposit number is BCRC 21815, this mixed powder and this yeast A weight-to-volume ratio of a bacterial solution is 2-20:5-10 (g:mL), and a bacterial cell concentration of the bacterial solution is 10 ⁵ CFU/mL to 10 ⁸ CFU/mL. The manufacturing method of magnolia flower oat leaven as claimed in claim 1, further comprising at least performing a drying step and a grinding step on the magnolia flower and the oat bran to obtain the mixed powder, wherein a temperature of the drying step is to not exceed 80°C. The manufacturing method of magnolia oat fermented product as claimed in claim 2, wherein a particle size of the mixed powder is 30 mesh to 80 mesh. The manufacturing method of the magnolia oat fermented product according to claim 1, further comprises a solid-liquid separation step and/or a sterilization step after the fermenting step. A magnolia flower oat ferment, wherein the magnolia oat ferment is obtained by the manufacturing method described in any one of claim 1 to claim 4, and each milliliter of the magnolia oat ferment comprises 1.0 mg to 5.0 mg of Protein, 5.0 mg to 15.0 mg of crude polysaccharides, 1.0 mg to 1.8 mg of total flavonoids, and 0.1 mg to 0.5 mg of total phenols. The magnolia flower oat starter according to claim 5, wherein the pH value of the magnolia flower oat starter is 4.5 to 6.8. The magnolia flower oat fermented product as claimed in claim 5, wherein a viscosity of the magnolia flower oat fermented product is 10cP to 15cP. An external composition for improving skin redness symptoms, comprising the magnolia oat leaven as described in any one of claim 5 to claim 7 as an active ingredient. The external composition for improving skin redness symptoms according to claim 8, wherein the magnolia flower oat leaven has antioxidant capacity. The topical composition for improving skin redness symptoms according to claim 8, wherein one dose of the topical composition per square centimeter of the skin of the affected part is 2.0 mg.

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