TWI768873B - Test tubes for loading biological samples - Google Patents

Abstract

A test tube for loading biological samples, comprising a tube body integrally formed by plastic material, and an upper cover detachably arranged on the tube body. The pipe body includes a pipe wall and an extension wall, the pipe wall has an inner pipe surface defining a pipe channel, and the pipe channel has a pipe opening communicating with the outside. The extension wall is located in the tube channel, the extension wall is connected to the inner tube surface and defines a grinding chamber with a closed bottom side, the top side of the grinding chamber communicates with the tube channel, the extension wall It has an inner wall surface surrounding the grinding chamber, the inner wall surface has a grinding surface, and the grinding surface has a rough structure. With the rough structure, the biological sample can be directly ground in the grinding chamber without changing the tube, which can simplify the processing flow of the biological sample.

Description

Test tubes for loading biological samples

The present invention relates to a test tube, in particular to a test tube for loading biological samples.

The existing biological specimens are stored in a test tube containing a solvent after collection and inspection, and are transported to a laboratory for further analysis and inspection after being sealed and stored. However, before using the instruments and equipment for analysis and testing, the biological sample must be taken out from the aforementioned test tube, and then put into a glass tube to grind and break the tissue of the biological sample, so as to refine the biological sample, and then put it in a glass tube. Into the instrument for analysis, which can improve the accuracy of the analysis.

However, the process of taking the biological specimen out of the test tube and putting it into the glass tube is cumbersome and easy to contaminate the biological specimen, which affects the interpretation of the test results.

Therefore, the object of the present invention is to provide a test tube for loading biological samples, which can simplify the biological sample processing process.

Therefore, the test tube used for loading biological samples according to the present invention includes a tube body integrally formed from a plastic material, and an upper cover. The pipe body includes a pipe wall and an extension wall, the pipe wall has an inner pipe surface surrounding an axis and defining a pipe channel, the pipe channel extends along the direction of the axis and has a nozzle communicating with the outside. The pipe body has a top end and a bottom end oppositely arranged on the axis, and the nozzle is located at the top end. The extension wall is located in the tube channel, the extension wall is connected to the inner tube surface and defines a grinding chamber with a closed bottom side, the top side of the grinding chamber communicates with the tube channel, the extension wall It has an inner wall surface surrounding the grinding chamber, the inner wall surface has a grinding surface, and the grinding surface has a rough structure. The upper cover is detachably disposed on the pipe body and used to close the pipe opening.

The effect of the present invention is that: by the design of the rough structure of the grinding surface, friction force can be generated on the biological sample to fix its position, and it is not easy to slip off, and the uneven surface of the rough structure can also cut the biological sample. The biological sample can be refined and homogenized by the force and stirring force. Therefore, after the biological sample is put into the tube body, the upper cover can be tightly closed for good preservation, and it is also easy to carry or transport. For inspection and analysis, the biological sample can be directly ground in the grinding chamber without changing the tube by only opening the upper cover, thereby simplifying the processing flow of the biological sample and preventing the biological sample from being contaminated.

Referring to FIG. 1 to FIG. 3 , an embodiment of the test tube for loading biological samples according to the present invention is suitable for pre-filling a solvent 91 , then loading a biological sample 92 during sampling, and then using a grinding rod 93 for the biological sample The sample 92 is ground and pulverized to facilitate nucleic acid extraction. In the following description, the biological sample 92 is described as a block-shaped biological soft tissue, but the biological sample 92 can also be other shapes or biological hard tissue, which is not limited to this. . When in use, the present invention can use the grinding rod 93 for manual grinding, or use a machine to grind automatically, and the usage mode is not a limitation of the present invention. The test tube for loading biological samples includes a tube body 1 and an upper cover 2 .

The tube body 1 is integrally formed from a transparent plastic material. The tube body 1 has a top end 101 and a bottom end 102 which are oppositely disposed on an axis L. The pipe body 1 includes a pipe wall 11 and an extension wall 12 . The tube wall 11 has an inner tube surface 111 around the axis L, an outer tube surface 112 opposite to the inner tube surface 111 , and a bottom surface 110 connected to the inner tube surface 111 and the outer tube surface 112 . The inner tube surface 111 defines a tube channel 100 . The tube channel 100 extends along the direction of the axis L. The tube channel 100 has a nozzle 103 located at the top end 101 and communicated with the outside world, and a bottom opening 104 located at the bottom end 102 and opposite to the nozzle 103 . . In some variations, the bottom end 102 of the tube channel 100 may be closed without the bottom opening 104 . The outer pipe surface 112 is formed with a threaded portion 113 located at the top end 101, and at least one mark 114 located between the top end 101 and the bottom end 102. As shown in FIG. 2, the number of the marks 114 is four and is equally spaced Arrangement, each of the markings 114 can be used as a guide for the volume by forming a semi-transparent fog surface around a circle with a fine bite flower. In some variations, each of the markings 114 can also be a ridge, groove or As long as the bumps can be clearly identified by the operator. Since the tube body 1 is made of transparent plastic material, it is convenient for the operator to see the contents. In some variations, the threaded portion 113 and the markings 114 can also be formed on the inner tube surface 111 , or even omitted. The bottom surface 110 is located at the bottom end 102 . With the arrangement of the bottom surface 110 , the tube body 1 can be placed on a surface (not shown) such as but not limited to a table top stably and upright for ease of use.

The extension wall 12 is located in the tube channel 100, the extension wall 12 partitions the tube channel 100 into a sample chamber 105 and a bottom chamber 106, the sample chamber 105 communicates with the nozzle 103, the bottom chamber 106 communicates with the bottom opening 104 . In some embodiments, the extension wall 12 extends obliquely from the inner tube surface 111 toward the axis L and surrounds and defines a grinding chamber 120 with a closed bottom side, the top side of the grinding chamber 120 and the pipe channel The sample chamber 105 of 100 is in communication. In some variations, the bottom chamber 106 can also be omitted in this embodiment, that is, the bottom surface 110 is connected to the connecting surface 123 of the extension wall 12, and the extension wall 12 is exposed. At this time, only the pipe body 1 Placed in a test tube rack (not shown) for positioning without tipping.

大於該研磨面部124與該軸線L的夾角

，但在其他變化例中，該連接面部123與該軸線L的夾角

也能小於該研磨面部124與該軸線L的夾角

，只要該夾角

與該夾角

不相同而能構成轉折面即可。在一實施例中，該研磨容室120以該軸線L為中心對稱設置並呈圓錐狀，該研磨容室120具有一位於該研磨面部124的低點125，該低點125供該軸線L通過。在一些變化例中，該研磨面部124也可僅形成於該內壁面121的環側。在又一些變化例中，該研磨容室120也能呈圓柱狀，且該研磨面部124可形成於該內壁面121的底側或環側。本實施例藉由該連接面部123的設置，可導引該研磨棒93滑入該研磨容室120，並獲得較佳的施力角度。該連接面部123例如是但不限於光滑表面，在處理過程中，可使溢出於該研磨容室120的該生物樣本92較容易再滑回該研磨容室120內，因此，該生物樣本92可被集中於該研磨容室120，不會亂跑，研磨效果較佳。 Referring to FIGS. 2 to 4 , the extension wall 12 surrounds the axis L and allows the axis L to pass through. The extension wall 12 has an inner wall surface 121 surrounding the grinding chamber 120 , and an inner wall surface 121 opposite to the inner wall 121 . The outer wall surface 122 of the wall surface 121 . The inner wall surface 121 has a connecting surface 123 extending obliquely from the inner tube surface 111 to the direction of the axis L, and a grinding surface 124 extending obliquely from the connecting surface 123 to the direction of the bottom opening 104. In some modifications Among them, the connecting surface 123 may be omitted from the inner wall surface 121 . In one embodiment, the included angle between the connecting surface 123 and the axis L is

greater than the angle between the grinding surface 124 and the axis L

, but in other variations, the angle between the connecting surface 123 and the axis L

It can also be smaller than the angle between the grinding surface 124 and the axis L

, as long as the angle

with this angle

It suffices to be different and to form a turning surface. In one embodiment, the grinding chamber 120 is symmetrically arranged around the axis L and has a conical shape. The grinding chamber 120 has a low point 125 located on the grinding surface 124, and the low point 125 allows the axis L to pass through. . In some variations, the grinding surface portion 124 may also be formed only on the annular side of the inner wall surface 121 . In still other variations, the grinding chamber 120 can also be cylindrical, and the grinding surface 124 can be formed on the bottom side or the ring side of the inner wall surface 121 . In this embodiment, by the arrangement of the connecting surface 123, the grinding rod 93 can be guided to slide into the grinding chamber 120, and a better force application angle can be obtained. The connecting surface 123 is, for example, but not limited to, a smooth surface. During processing, the biological sample 92 overflowing from the grinding chamber 120 can be easily slid back into the grinding chamber 120. Therefore, the biological sample 92 can be Being concentrated in the grinding chamber 120 , it will not run around, and the grinding effect is better.

The grinding surface 124 has a rough structure 126 , by which the biological sample 92 can be directly ground in the grinding chamber 120 (see FIG. 2 ). The rough structure 126 in this embodiment comes from the transfer of the mold surface , and the common processing methods of the mold surface include polishing, electric discharge embossing, potion embossing and laser embossing. In other variations, the rough structure 126 can also be formed by sandblasting, laser engraving, electrical discharge machining, and chemical etching. As long as a regular or irregular and fine concave-convex surface can be produced, it has a grinding effect. It should be noted that, in order to avoid that part of the tissue of the biological sample 92 is stuck in the rough structure 126 after grinding and cannot be taken out completely, therefore, the rough structure 126 needs to present a fine concave-convex surface so as not to get stuck after grinding. However, the present invention does not limit the geometry and/or distribution area of the rough structure 126 . In actual use, if the grinding rod 93 matched with the shape and size of the grinding chamber 120 can be matched, or the front end of the grinding rod 93 can be formed with an embossed structure, the grinding effect can be improved, and it has the advantage of saving labor. .

Referring to FIG. 3 , FIG. 5 and FIG. 6 , the upper cover 2 is detachably screwed to the pipe body 1 and used to close the nozzle 103 . The upper cover 2 is integrally formed of plastic material and has an inner ring wall 21 and an outer ring wall 22 that surround the axis L and are spaced apart. The inner ring wall 21 and the outer ring wall 22 cooperate to form a full circumference The annular groove 23 surrounds the inner annular wall 21 and the outer annular wall 22 , and the annular groove 23 allows the top end 101 of the pipe wall 11 to penetrate and engage. In one embodiment, the inner surface of the outer annular wall 22 has a helical portion 221 facing the annular groove 23 , and the helical portion 221 is used for screwing to the threaded portion 113 of the pipe body 1 . In one embodiment, the outer surface of the outer ring wall 22 has a non-slip structure formed by a combination of several protruding strips 222 parallel to the axis L. When the upper cover 2 is screwed to the tube body 1, the protruding strips 222 can prevent the hand from slipping off during the tightening process. The top end 101 of the pipe wall 11 penetrates the annular groove 23 and the helical portion 221 is engaged with the threaded portion 113 to prevent the solvent 91 in the pipe body 1 from leaking out and prevent leakage. In some variations, the plastic can also be elastically deformed, and the width of the annular groove 23 is slightly smaller than the thickness of the pipe wall 11, so that the pipe body 1 and the upper cover 2 are joined in a tight fit. Similarly The upper cover 2 can be detachably arranged on the tube body 1 and has the function of being convenient for storage. Therefore, the present invention does not limit the connection method and/or position of the tube body 1 and the upper cover 2 .

Referring to FIGS. 5 and 7 , the helical portion 221 of the upper cover 2 is a double-threaded structure, the inner ring wall 21 has at least one protruding portion 211 facing the helical portion 221 , and the at least one protruding portion 211 is matched with the helical portion 211 The two sides are respectively pressed against the pipe wall 11 of the pipe body 1 to increase the watertight effect and prevent the solvent 91 (see FIG. 6 ) from leaking out during storage or transportation. The at least one convex portion 211 can be a convex point, a convex strip or a convex block.

Therefore, when the specimen is collected, the upper cover 2 of the present invention is first opened, and the collected biological sample 92 is put into the sample chamber 105 of the tube body 1, and the biological sample 92 will be submerged in the solvent 91 to be protected, and then screw the upper cover 2 to the tube body 1 to achieve a watertight effect to ensure that the solvent 91 and the biological sample 92 will not leak. Next, the present invention is carried or transported to a testing room (not shown), the upper cover 2 is opened, and the grinding rod 93 is used to extend into the grinding chamber 120 for tissue grinding and refining. In the present invention, the biological sample is always in the tube body 1 during the process of sampling, storage, transportation, and tissue grinding and refinement in the laboratory, and there is no need to change the tube. Therefore, the present invention can simplify the biological sample 92 process, saving processing time. In addition, since the tube body 1 of the present invention is made of plastic material, it has the advantages of light weight and no breakage, which is not only convenient to take, but also improves the safety of use.

To sum up, the test tube used for loading biological samples of the present invention utilizes the concave-convex surface of the rough structure 126 of the grinding surface 124 to generate frictional force for the biological sample 92 to fix its position without slipping off easily. The concave-convex surface of the rough structure 126 generates shearing force and stirring force on the biological sample 92, so that the biological sample 92 can be refined and homogenized. Therefore, in the present invention, after the sampled biological sample 92 is put into the tube body 1, the upper cover 2 can be tightly closed for good preservation, and it is also convenient to carry or transport. When the inspection and analysis are to be performed, the biological sample 92 can be processed directly in the grinding chamber 120 by only opening the upper cover 2 without changing the tube, which can simplify the processing flow of the biological sample 92 and avoid the biological sample 92. Sample 92 is contaminated. In addition, since the tube body 1 of the present invention is made of plastic material, it has the advantages of light weight and no breakage, which is not only convenient to take, but also improves the safety of use. In this embodiment, through the inclined design of the extension wall 12 of the tube body 1 , the concentration effect of the biological sample 92 in the grinding chamber 120 can be improved, so that the grinding effect is better, so the object of the present invention can be achieved.

However, the above are only examples of the present invention, and should not limit the scope of implementation of the present invention. Any simple equivalent changes and modifications made according to the scope of the patent application of the present invention and the contents of the patent specification are still included in the scope of the present invention. within the scope of the invention patent.

1: Tube body

100: Tube channel

101: Top

102: Bottom

103: Nozzle

104: Bottom opening

105: Sample Chamber

106: Bottom chamber

11: Pipe wall

110: Bottom

111: inner tube surface

112: Outer tube surface

113: Threaded part

114: Mark

12: Extension Wall

120: Grinding chamber

121: inner wall surface

122: outer wall

123: Connecting the Face

124: Grinding the face

125: low

126: Rough Structure

2: upper cover

21: Inner ring wall

211: convex part

22: Outer ring wall

221: Spiral

222: Rib

23: Ring groove

:夾角

: included angle

:夾角

: included angle

L: axis

91: Solvent

92: Biological samples

93: Grinding rod

Other features and effects of the present invention will be clearly presented in the embodiments with reference to the drawings, wherein: 1 is an exploded perspective view of an embodiment of a test tube for loading a biological sample according to the present invention; Fig. 2 is a use schematic diagram of this embodiment; Fig. 3 is a sectional schematic diagram of this embodiment; Fig. 4 is a partial cross-sectional enlarged view of this embodiment; Fig. 5 is a sectional view of this embodiment; Figure 6 is a schematic front view of a tube body of this embodiment pre-filled with a solvent; and FIG. 7 is an incomplete cross-sectional view of an upper cover of this embodiment.

1: Tube body

100: Tube channel

101: Top

102: Bottom

103: Nozzle

104: Bottom opening

105: Sample Chamber

106: Bottom chamber

11: Pipe wall

111: inner tube surface

112: Outer tube surface

113: Threaded part

12: Extension Wall

120: Grinding chamber

126: Rough Structure

2: upper cover

21: Inner ring wall

22: Outer ring wall

221: Spiral

23: Ring groove

L: axis

Claims (10)

A test tube for loading biological samples, comprising: a tube body, which is integrally formed by plastic material, the tube body includes a tube wall and an extension wall, the tube wall has a tube surrounding an axis and defines a tube channel The inner pipe surface of the pipe channel extends along the direction of the axis and has a nozzle communicating with the outside world, the pipe body has a top end and a bottom end oppositely arranged on the axis, and the nozzle is located at the top end, The extension wall is located in the tube channel, the extension wall is connected to the inner tube surface and defines a grinding chamber with a closed bottom side, the top side of the grinding chamber communicates with the tube channel, the extension wall There is an inner wall surface surrounding the grinding chamber, the inner wall surface has a grinding surface, the grinding surface has a rough structure, the extension wall also has a connecting surface connected to the grinding surface and extending to the inner pipe surface, the The included angle between the connecting surface and the axis is not equal to the included angle between the grinding surface and the axis; and an upper cover, which is detachably disposed on the pipe body and used to close the nozzle. The test tube for loading biological samples according to claim 1, wherein the extending wall is inclined and extended toward the axis from the inner tube surface, and the grinding surface of the inner wall surface surrounds the axis. The test tube for loading biological samples as claimed in claim 2, wherein the grinding chamber has a low point located on the grinding surface, and the low point allows the axis to pass through. The test tube for loading biological samples according to claim 1, wherein the tube wall further has an outer tube surface opposite to the inner tube surface, and a tube connected to the inner tube surface and the outer tube surface Bottom surface, the bottom surface is located at the bottom end and is used to stand on a surface. The test tube for loading biological samples according to claim 1, wherein the tube wall further has an outer tube surface opposite to the inner tube surface, the outer tube surface is formed with a threaded portion located at the top end, the The threaded portion is used for the detachable screw connection of the upper cover. The test tube for loading biological samples according to claim 1, wherein the tube channel further has a bottom opening opposite to the mouth of the tube, and the extension wall partitions the tube channel into a sample chamber and a bottom a chamber, the sample chamber communicates with the nozzle, and the bottom chamber communicates with the bottom opening. The test tube for loading biological samples according to claim 1, wherein the upper cover has an inner ring wall and an outer ring wall surrounding the axis and arranged at intervals, and the inner ring wall and the outer ring wall cooperate together A ring groove is formed around the entire circumference between the inner ring wall and the outer ring wall, and the ring groove is used for the pipe wall to penetrate and join. The test tube for loading biological samples according to claim 7, wherein the outer ring wall has a helical portion for screwing the tube wall, and the inner ring wall has at least one convex portion facing the helical portion, The at least one convex portion is matched with the helical portion and pressed against the pipe wall of the pipe body, thereby increasing the watertight effect. The test tube for loading biological samples according to claim 1, wherein the included angle between the connecting surface and the axis is greater than the included angle between the grinding surface and the axis. The test tube for loading a biological sample according to claim 1, wherein the connecting face is a smooth surface.

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