TWI652061B - Use of a composition containing ginsenoside Mc and ginsenoside Mc as an active ingredient - Google Patents

Abstract

The present invention relates to a ginsenoside-containing Mc, which can provide anti-aging, improve skin wrinkles, whitening, moisturizing improvement effects and anti-inflammatory, improve acne and skin problems, improve the effects of specific reactive symptoms, skin convergence and pore shrinkage effect It can also provide a composition for improving skin color, promoting hair growth, improving white hair, anti-dandruff and antiseptic effect.

Description

Composition containing ginsenoside Mc and use of ginsenoside Mc as active ingredient

The present invention relates to a ginsenoside-containing Mc, which can provide anti-aging, improve skin wrinkles, whitening, moisturizing improvement effects and anti-inflammatory, improve acne and skin problems, improve the effects of specific reactive symptoms, skin convergence and pore shrinkage effect It can also provide a composition for improving skin color, promoting hair growth, improving white hair, anti-dandruff and antiseptic effect.

Human skin is the first protective film of the human body. It plays a role in protecting the organs of the body during the stimulation of external environments such as temperature and humidity changes, ultraviolet rays, and harmful substances. Causes change. That is, in terms of internal factors, the secretion of various hormones used to regulate metabolism is reduced, the function of immune cells and cell activity are reduced, resulting in a decrease in the biosynthesis of immune proteins and body structural proteins required by the human body; in terms of external factors Due to the destruction of the ozone layer, the amount of ultraviolet rays reaching the surface of the sun's rays has increased, and as environmental pollution has gradually intensified, free radicals and active oxygen have increased, resulting in reduced skin thickness, increased wrinkles, and reduced elasticity. As a result, skin complexion becomes dull, skin problems often occur, melasma and freckles and age spots are increased, poor complexion becomes darker, skin tone becomes darker, etc., resulting in various changes.

In order to prevent changes in the skin state caused by internal and external causes of this kind of skin and to maintain a healthy skin state, try adding bioactive substances extracted from various known animals, plants, microorganisms, etc. to cosmetics and use them. Improve skin condition.

Ginsenoside Mc (Ginsenoside Mc) is a natural form of ginsenoside (Rb1, Rg1, Rd, Rb2, Rc, Rf) contained in ginseng. It is decomposed and produced by digestive enzymes and intestinal microorganisms. Its strong anticancer activity has been characterized. Published, Korean Registered Patent No. 164266 describes a method for its use as an anticancer drug, and the overall skin condition improving effect of a composition containing ginsenoside Mc as an active ingredient, as well as promoting hair growth and improving whiteness have not been disclosed. Hair, anti-dandruff and antiseptic are the overall effects of skin external preparations.

[Problems to be solved by the invention]

In response, the present inventors have discovered that ginsenoside Mc can provide anti-aging, skin wrinkle, whitening, moisturizing improvement effects, anti-inflammatory, acne and skin problems, and improve the symptoms of specific reactions, as well as skin color improvement , Promote hair growth, improve white hair, anti-dandruff and anti-corrosion effects, thus completing the present invention.

Therefore, an object of the present invention is to provide a skin external preparation composition which exhibits improvement of the overall state of the skin and promotes hair growth, improves white hair, has antidandruff and antiseptic effects by containing ginsenoside Mc. [Technical means to solve the problem]

In order to achieve the above object, the present invention provides an anti-aging skin external preparation composition containing ginsenoside Mc as an active ingredient. The present invention also provides a skin external preparation composition for improving wrinkles containing ginsenoside Mc as an active ingredient. The present invention also provides a moisturizing skin external preparation composition containing ginsenoside Mc as an active ingredient. The present invention also provides a skin external preparation composition for improving acne containing ginsenoside Mc as an active ingredient. In addition, the present invention provides a skin external preparation composition for improving color and skin tone containing ginsenoside Mc as an active ingredient. The present invention also provides a skin external preparation composition for pore shrinkage containing ginsenoside Mc as an active ingredient. The present invention also provides a skin external preparation composition for skin with specific reaction containing ginsenoside Mc. The present invention also provides an anti-inflammatory skin external preparation composition containing ginsenoside Mc. The present invention also provides a skin external preparation composition for whitening containing ginsenoside Mc. The present invention also provides a composition for promoting hair growth containing ginsenoside Mc. The present invention also provides a composition for preventing gray hair containing ginsenoside Mc. The present invention also provides an antidandruff composition containing ginsenoside Mc. The present invention also provides a natural preservative composition containing ginsenoside Mc. [Effect of the invention]

By containing ginsenoside Mc, the composition of the present invention can not only provide anti-aging, improve skin wrinkles, whitening, moisturizing improvement effects and anti-inflammatory, improve acne and skin problems, improve the effects of specific reactive symptoms, skin convergence and pore shrinkage Effect, can also provide skin color improvement effect, promote hair growth, improve white hair, anti-dandruff and antiseptic effect.

The composition according to the present invention contains ginsenoside Mc (ginsenoside Mc) as an active ingredient.

The ginsenoside Mc used in the present invention has a structure of the following Chemical Formula 1.

[Chemical Formula 1]

The ginsenoside Mc of the present invention can be extracted from plants, can also be synthesized and used in accordance with the methods disclosed in the art, and commercially available ginsenoside Mc can also be used. Ginsenoside Mc can be obtained from ginseng extract. At this time, the type of ginseng used is not particularly limited, and water ginseng, red ginseng, white ginseng, Taiji ginseng, and tail ginseng can be used. In addition, the above-mentioned ginseng extract includes all extracts obtained by leaching and decocting from ginseng, concentrates obtained by re-concentrating part or all of the extracts, or preparations by re-drying the above-mentioned concentrates The sinking body, decoction, tincture, fluid extract and chemicals contained in ginseng which exert its main effect, including the plant itself, the extract can be taken from all parts of ginseng such as stems, roots, leaves, flowers, fruits, etc. , Not limited to extracts from a specific part. In addition, as a method for extracting ginsenoside Mc from a ginseng extract, a public method can be used.

Specifically, after the ginseng extract is extracted from ginseng with water or an organic solvent by a method well known in the art, the aforementioned ginsenoside Mc can be isolated. The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, diethyl ether, ethyl acetate, chloroform, and a mixed solvent of these organic solvents and water. Preferably, 80% ethanol is used. At this time, preferably, the extraction temperature is 10 ° C to 80 ° C, and the extraction can be performed for 3 hours to 24 hours. If the above extraction temperature and extraction time are exceeded, the extraction efficiency will decrease or the composition will change.

Preferably, in the composition of the present invention, the content of the ginsenoside Mc is 0.001% to 50% by weight based on the total weight of the composition. If the content of the active ingredient is less than 0.001% by weight, the efficacy and effect of the component are weak; if the content of the active ingredient exceeds 50% by weight, problems in skin safety or dosage form may occur.

The composition of the present invention can be used as an anti-aging skin external preparation composition, and has the effects of increasing skin elasticity and improving wrinkles.

The composition of the present invention can be used as a moisturizing skin external preparation composition, which can strengthen the skin barrier function and induce the differentiation of skin keratinocytes. Thereby, it can be effectively used as a skin external preparation composition, thereby preventing or improving dry skin, atopic dermatitis, contact dermatitis, or scabies caused by incomplete epidermal differentiation.

The composition of the present invention can be used as a skin external preparation composition for improving acne, and has excellent antibacterial effect, especially has outstanding antibacterial effect on acne-causing bacteria, and it also provides anti-inflammatory effect.

The composition of the present invention can be used as a skin external preparation composition for improving color and complexion. After being applied to the skin, it can ensure smooth absorption of nutrients by expanding the capillaries and promoting blood circulation. Improves skin tone.

The composition of the present invention can be used as a skin external preparation composition for pore shrinkage, sebum regulation, and improvement of skin problems. After being applied to the skin, it suppresses sebum secreted due to excess, promotes elimination of active oxygen and synthesis of collagen, As a result, the pores are contracted, and the effect of curbing skin problems is reduced by the reduction of the expression of inflammatory factors.

The composition of the present invention can be used as a specific-reactive skin-improving composition, which not only provides excellent properties by inhibiting the activity of Proteinase-Activated Receptor-2 (PAR-2) which induces itching. The anti-pruritic effect can also provide an anti-inflammatory effect by reducing the secretion of Interleukin-8 (IL-8). Therefore, the above-mentioned ginsenoside Mc of the present invention is reasonable as an active ingredient of a skin external preparation composition for stabilizing sensitive, irritating or specifically reactive skin and scalp, and for improving or alleviating pruritus, fever symptoms and inflammation use.

The composition of the present invention can be used as a whitening composition, which can provide excellent whitening effects by inhibiting tyrosinase activity and suppressing melanin production.

The composition of the present invention can be used as a composition for promoting hair growth, which promotes the growth of hair and the generation of new hair by promoting the hair cycle from the resting phase to the hair growth phase, and can also ensure the healthy growth of existing hair and prevent And curb the state of finding elephants or thinning hair.

The composition of the present invention can be used as a composition for preventing white hair, which can increase the melanocyte activation and promote the melanin synthesis by increasing the expression of the small eye malformation-related transcription factor (MITF) of melanocytes. Prevent the formation of white hair and promote the growth of black hair.

The composition of the present invention can be used as an anti-dandruff skin external preparation composition, which can effectively excrete toxins accumulated in hair and scalp, purify the scalp, suppress the proliferation and growth of dandruff bacteria, and thus prevent scalp inflammation reactions In addition, its outstanding anti-oxidant effect in inhibiting the generation and action of active oxygen can provide the effect of soothing and strengthening the scalp and strengthening the inherent defense ability.

The composition of the present invention can be used as a natural preservative composition. It contains natural ingredients, and has excellent antiseptic effect and provides harmless effect to human body.

The composition according to the present invention can be formulated by including a cosmetically or dermatologically acceptable medium or mechanism. It is suitable for all dosage forms suitable for topical application, for example, emulsions, suspensions, microemulsions, microcapsules, and fine particle spheres that can be obtained as solutions, gels, solids, stirred anhydrous products, and dispersion of milky water. Or ionic (liposomes) and non-ionic vesicular dispersants, or they can be in the form of creams, lotions, lotions, powders, ointments, sprays or concealers provide. Alternatively, it can be used in the form of a foam (foam) or an aerosol composition containing a compressed propellant. These compositions can be prepared according to the usual methods in this field.

In particular, if the skin external preparation composition of the present invention is used as an antidandruff, hair growth or gray hair prevention application, it can be formulated into a composition for scalp and hair, and the dosage form is not particularly limited. For example, it can be a dosage form Turn into conditioner, hair nourishing water, softener, hair cream, shampoo, hair cream, hair cream or scalp hair dual-use care cream.

In addition, the composition according to the present invention may contain a fatty substance, an organic solvent, a dissolving agent, a concentrating agent, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, and a surface. Active agents, water, ionic or non-ionic emulsifiers, fillers, chelating agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilicity Or lipophilic active agents, lipid vesicular or adjuvants commonly used in the field of cosmetics or dermatology as any other ingredient commonly used in cosmetics. The amount of the above-mentioned adjuvant is based on the amount usually used in the field of cosmetics or dermatology.

In addition, the composition of the present invention may contain a skin absorption promoting substance in order to increase the skin improving effect.

In the following, test examples and formulation examples will be exemplified to more specifically describe the structure and effects of the present invention. However, these test examples and formulation examples are provided for the purpose of understanding the present invention, and the scope and scope of the present invention are not limited to the following examples.

[Reference Example 1] Preparation of ginsenoside Mc Ginsenoside Mc used to test the efficacy of the composition of the present invention was purchased from the AMBO Institute.

[Experimental Example 1] Elastase Activity Inhibitory Efficacy The elastin saponin Mc's elastase activity inhibiting ability was measured by comparison with epigallocatechin gallate (EGCG). The elastase and matrix used were purchased commercially from Sigma-Aldrich (Cat. No. E0127). The inhibitory effect of elastase activity was tested by the following test method. In a 96-well plate, 200 μl of ginsenoside Mc and 50 μL of 20 μg / mL elastase • type III solution were mixed in 10 mg / L of trismethyl chloride hydrochloride (Tris-HCl) buffer (pH 8.0). . Epigallocatechin gallate (EGCG) 250 μM was used as a positive control group, and non-treated group as a negative control group was distilled water. Then, 100 μL of 0.4514 mg / mL N-SUCCINYL-ALA-ALA-ALA-p-NITROANILIDE prepared with the above-mentioned buffer solution was added, and the reaction was performed at 25 ° C. for 15 minutes. After the reaction was completed, the absorbance was measured at a wavelength of 415 nm (Synergy2, BioTek (VT, USA)). A blank test was performed by the same method to correct it. The method for calculating elastase activity inhibitory effect is shown in Formula 1 below, and the results are shown in Table 1 below.

[Formula 1] Elastase activity inhibition rate (%) = {1- (CD) / (AB)} × 100 A: Absorptivity at a wavelength of 415 nm without addition of the test substance and enzyme: B: No addition of the test substance 2. Absorbance C at the wavelength of 415nm in the absence of enzyme addition C: Absorbance at the wavelength of 415nm in the addition of test substance D: Absorbance at wavelength 415nm in the addition of test substance and no enzyme

[Table 1] From the results in Table 1 above, it can be seen that the degree of inhibition of elastase activity of ginsenoside Mc is significantly better than that of epigallocatechin gallate (EGCG) as an inhibitor of elastase activity. The ginsenoside Mc of the invention has excellent inhibitory effect on elastase activity.

[Test Example 2] Collagenase (MMP-1) inhibitory ability The collagenase production inhibitory ability of the ginsenoside Mc of the present invention was measured by comparison with vitamin A acid (Retinoic acid). Add human fibroblasts to a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal bovine serum. In order to reach 5,000 cells / well, the cells were cultured in an incubator with 5% carbon dioxide and 37 ° C until the growth rate was about 70 to 80%. After ginsenoside Mc or Retinoic acid was treated at a concentration of 10 μg / ml for 24 hours, the cell culture solution was extracted. The degree of collagenase production of the extracted cell culture fluid was measured by a commercially available collagenase measuring instrument (Anfamaxia Biotech, Catalog #: RPN 2610). First, the extracted cell culture solution was placed in a 96-well plate, which was evenly coated with a collagenase antibody once, and an antigen-antibody reaction was performed in a thermostatic bath for 3 hours. After 3 hours, a secondary collagen antibody bound with a chromophore was added to a 96-well plate and reacted again for 15 minutes. After 15 minutes, add a hair color-inducing substance (3,3 ', 5,5'tetramethylbenzidine, sigma), induce color at room temperature for 15 minutes, add 1M sulfuric acid again to stop the hair color reaction, and the color of the reaction solution It is yellow, and the degree of yellow display varies according to the progress of the reaction. The absorbance of a yellow 96-well plate was measured at 405 nm by an absorbance meter, and the degree of collagenase synthesis was calculated by the following formula 2. The results are shown in Table 2. At this time, the reaction absorbance of the cell culture fluid extracted from the group not treated with the composition was used as a control group.

[Formula 2] The degree of collagenase expression (%) = absorbance of the substance-treated cell group / absorbance of the control group × 100

[Table 2] From the results in Table 2 above, it can be seen that the degree of collagenase expression of ginsenoside Mc is similar to that of vitamin A acid (retinoic acid), which is a collagenase inhibitor, in a similar degree to its collagenase inhibitory effect. From these results, it was confirmed that the ginsenoside Mc of the present invention has an effect of inhibiting matrix metalloproteinase (MMP-1).

[Formulation Example 1 and Comparative Formulation Example 1] A nutrition cream (unit: weight%) was prepared by a general method according to the composition of Table 3 below.

[table 3]

[Test Example 3] Confirmation of skin elasticity-enhancing effect In order to confirm the skin elasticity-enhancing effect on the human body, the above-mentioned formulation example 1 and comparative formulation example 1 were evaluated as follows. Twenty healthy women between the ages of 30 and 40 were divided into two groups of 10 in each group, which corresponded to the two groups of Formulation Example 1 and Comparative Formulation Example 1, respectively, and the nutrition cream was applied to the subjects On the face, skin elasticity was measured with a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany) once a day for 12 weeks. The results are shown in Table 4 below. The results shown in Table 4 are described by the ΔR8 value of Cutometer SEM 575, and the R8 value indicates the properties of skin viscoelasticity.

[Table 4] From the results in Table 4 above, it can be seen that the dosage form example 1 containing the ginsenoside Mc of the present invention has a further increase in skin elasticity as compared with the case of applying the comparative dosage form example 1. Therefore, it was confirmed that the composition containing the ginsenoside Mc of the present invention is very effective for increasing skin elasticity.

[Test Example 4] Confirmation of skin wrinkle improvement effect In order to confirm the wrinkle improvement effect by the composition of the present invention, the above-mentioned formulation example 1 and comparative formulation example 1 were used. In order to confirm the wrinkle improvement effect of the above-mentioned dosage form example 1 and comparative dosage form example 1, evaluation was performed as follows. Twenty healthy women in the 40-year-old age group were divided into two groups of 10 each, corresponding to the two groups of Formulation Example 1 and Comparative Formulation Example 1, respectively, and the nutrition cream was applied to the face of the subject For 12 weeks, once a day, the replicas were extracted with silicone, and measured with a skin measuring instrument (visiometer, SV600, Courage + Khazaka electronic GmbH, Germany) and image analysis was performed. The results are shown in Table 5 below. The values in Table 5 below represent the average value of the parameter values before excluding the parameter values after 12 weeks of application.

[table 5] From the results in Table 5 above, it can be seen that the composition for external use of Formulation Example 1 has an excellent effect of improving skin wrinkles.

[Test Example 5] Tyrosinase inhibitory effect Tyrosinase was extracted from mushrooms, and a tyrosinase prepared by SIGMA Corporation was used. First, tyrosine as a matrix was dissolved in distilled water to prepare a 0.3 mg / ml solution, and the solution was added to a test tube in an amount of 1.0 ml, and then 1.0 ml of a potassium-phosphate buffer solution (0.1 mol concentration) was added thereto. , PH 6.8) and 0.7ml of distilled water. 0.2 ml of a sample solution of ginsenoside Mc prepared by mixing at 10 ppm was added to the ethanol reaction solution of the present invention and reacted in a constant temperature bath at 37 ° C for 10 minutes. At this time, 0.2 ml of a solvent was used in place of each sample solution as a control group, and ascorbic acid was used as a positive control group. In the reaction solution, a 2500 unit / ml tyrosinase solution was added in 0.1 ml amounts each time, and reacted again in a constant temperature bath at 37 ° C for 10 minutes. The test tube containing the reaction solution was put into ice water, quickly cooled and the reaction was stopped, and the absorbance at a wavelength of 475 nm was measured by a photoelectric spectrometer (Synergy2, BioTek (VT, USA)). The results are shown in the table below. 6. Each tyrosinase inhibitory effect was calculated by the following formula 3.

[Formula 3] Tyrosinase inhibition rate (%) = 100- (Reaction absorbance of test substance / Reaction absorbance of control group) × 100

[TABLE 6] From the results in Table 6 above, the inhibition rate of tyrosinase of ginsenoside Mc according to the present invention is much higher than that of ascorbic acid known as a tyrosinase inhibitor, and it can be seen that its whitening effect is very excellent.

[Test Example 6] Based on the melanin production inhibiting effect of B16 / F10 melanoma cells, a sample containing 0.001% by weight of each of ginsenoside Mc and Kojic acid was used as a test substance, and it was added to B16 / F10 at a predetermined concentration. The culture solution of melanoma cells (Korean Cell Line Bank) was cultured for 3 days. The culture solution was removed, washed with phosphate buffered saline (PBS), and the cells were lysed with 1N sodium hydroxide (NaOH), and measured at 405 nm. Absorbance (Synergy2, BioTek (VT, USA)). The cells to which no test substance was added were used as a control group, and the melanin production inhibition degree of each test substance was measured by comparing the melanin content in the control group. The melanin production inhibition rate was calculated according to Equation 4, and the results are shown in Table 7.

[Formula 4] Inhibition rate of melanin production (%) = 100- (absorbance of test substance / absorbance of control group) × 100

[TABLE 7] From the results in Table 7 above, according to the ginsenoside Mc of the present invention, the inhibition rate of melanin production is much higher than that of Kojic acid, which is a known melanin production inhibitor, and it can be seen that the whitening effect is very excellent.

[Test Example 7] Measurement of skin moisturizing force increasing effect In order to measure the effect of ginsenoside Mc on increasing skin moisturizing force, the above-mentioned formulation example 1 and comparative formulation example 1 were used and evaluated as follows. Twenty adult men and women with dry skin types in the 40-50 age group were divided into two groups with 10 in each group, which corresponded to the two groups of Formulation Example 1 and Comparative Formulation Example 1, respectively. Apply on the subject's face twice a day for 12 weeks. Before the beginning of application, after 1 week of application, after 2 weeks of application, after 4 weeks of application, and after 2 weeks of application stop (6 weeks in total), under constant temperature and humidity conditions (24 ° C, 40% relative humidity), A skin moisture measuring instrument (Corneometer CM825, C + K Electronic Co., Germany) was used to measure skin moisture. The results are shown in Table 8 below. The results in Table 8 are based on the skin moisture measurement value measured on the eve of the start of the test, and the percentage increase in the measurement value after a predetermined period of treatment.

[TABLE 8] From Table 8 above, if Comparative Example 1 is applied, it will show a moisture increase rate of about 30% by the 4th week of application, and the skin moisture content will decrease after application is stopped. On the contrary, If the dosage form example 1 containing ginsenoside Mc is applied, after the application is stopped, most of the skin moisture increase rate is more than 30%. From this, it can be seen that the composition of the present invention containing ginsenoside Mc has excellent skin moisturizing effect.

[Test Example 8] Measurement of keratinocyte differentiation promotion effect In order to understand the keratinocyte differentiation promotion effect of ginsenoside Mc, CE (Cornified Envelop) generated during keratinocyte differentiation was measured by absorbance in the following manner. the amount. First, the keratinocytes of the human body that have been cultured once are put into a culture bottle and attached to the bottom. The keratinocytes are separated from the epidermis of the newborn. Then, the ginsenoside Mc is treated at a concentration of 5 ppm into the culture solution, Incubate for 5 days until the cells grow to about 70 to 80% of the bottom area. At this time, the low calcium (0.03 mM) treatment group and the high calcium (1.2 mM) treatment group were used as the negative control group and the positive control group, respectively. Then, extract the cultured cells, wash them with phosphate buffered saline (PBS), and then add 1 ml of 3% 10mM concentration containing 2% SDS (sodium dodecyl sulfate) and 20mM DTT (Dithiothreitol). Hydroxymethylaminomethane hydrochloride buffer solution (Tris-HCl, pH 7.4) and ultrasonication, boiling, centrifugation, and resuspending the precipitate in 1 ml of phosphate buffer solution (PBS), The absorbance was measured at 340 nm (Synergy2, BioTek (VT, USA)). In addition, the above-mentioned solution after partial ultrasonic treatment was extracted, and its protein content was measured, which was used as a reference when evaluating the degree of cell differentiation. The results are shown in Table 9.

[TABLE 9] From the results in Table 9 above, it was found that when ginsenoside Mc is used, the keratinocyte differentiation promoting effect is excellent.

[Test Example 9] Measurement of skin barrier function restoration effect In order to determine the effect of ginsenoside Mc on restoration of damaged skin barrier function due to skin injury, the following experiments were performed. Skin barrier damage was applied to the upper arms of 10 adult men and women by the Tape Stripping method, and two groups of the application form example 2 and the comparative form example 2 were prepared by the composition of the following table 10, respectively. Vapometer (Delfin, Finland) was used to measure and compare the recovery of transepidermal water loss rate (TWEL), once a day for 7 consecutive days. Among them, Comparative Formulation Example 2 is used as a negative control group, and it is a vehicle. The test results are shown in Table 11 below. The results in Table 11 are based on 100% and compare the difference in treatment before and after barrier damage.

[TABLE 10]

[TABLE 11] From the results in Table 11 above, if the comparative formulation example 2 containing no ginsenoside Mc is treated, the transepidermal water loss rate gradually increases over time; on the contrary, if the dosage form example 2 containing ginsenoside Mc is treated, The transepidermal water loss rate quickly returns to normal, and barrier damage is restored.

[Experimental Example 10] Gas-color improvement effect In order to evaluate the skin blood circulation promoting effect of the cosmetic composition according to the present invention, a laser Doppler Perfusion Imager (Laser Doppler Perfusion Imager; periscan PIM II, Perimed (stochholm, Sweden) )) And measure the blood circulation of the skin. Laser Doppler blood flow imager is a commonly known instrument for measuring blood circulation in the skin. It is a currently used instrument that can not only measure the speed and amount of blood in the capillaries of the skin, It can also measure the flow in small arteries and veins, which is a very sensitive device. After washing the face with soap in a constant temperature and humidity room, relax for 30 minutes, and measure the initial value with a laser Doppler blood flow imager. First, for 30 women who usually have cold hands and feet, the initial blood flow of the lower part of their forehead is measured by a laser Doppler blood flow imager. Next, the subjects were arranged to use the above-mentioned dosage form example 1 and the comparative dosage form example 1 within a period of one week, and compared the measured blood flow with the above-mentioned initial measurement value. The results (changes in skin blood flow) are shown in Table 12 below.

[TABLE 12] From the results in Table 12 above, the cosmetic composition according to the present invention significantly increased skin blood flow compared to Comparative Formulation Example 1 which did not contain ginsenoside Mc, and it was found that the appearance of the complexion was enhanced by such blood circulation promotion. Improved. In summary, this result indicates that the ginsenoside Mc-containing cosmetic composition according to the present invention can contribute to effectively transmitting the nutritional ingredients of the skin, suppressing and delaying skin aging.

[Experimental Example 11] Skin color improvement effect In order to understand the skin color improvement effect of the above formulation example 1 and comparative formulation example 1, 30 subjects were used separately (1 time / day for 1 week), with Facial Stage DM- 3 (Moritex, Japan) device to assess skin tone improvement. Regarding the skin color improvement rate, the skin lightness and color measurement values were measured and the change values were used as the skin color improvement rate. The results are shown in Table 13 below. The larger the brightness and color change values, the higher the improvement in skin tone.

[TABLE 13] From the results in Table 13 above, it can be seen that Comparative Formulation Example 1 which does not contain ginsenoside Mc according to the present invention does not show significant skin tone improving effect; on the contrary, Formulation Example 1 which contains ginsenoside Mc as an active ingredient Compared with the skin tone before use, the skin tone after use is greatly improved.

[Test Example 12] Pore shrinking effect 1. Pore shrinking effect by promoting collagen biosynthesis The collagen biosynthesis promoting effect of ginsenoside Mc according to the present invention was measured by comparison with TGF-β. First, fibroblasts were seeded into 24 wells at 105 cells per well, and cultured until they grew to about 90%. After culturing them in serum-free Dulbecco's modified Eagle medium for 24 hours, the ginsenoside Mc and TGF-β of the present invention dissolved in serum-free medium were treated with 10 ng / ml, respectively, and cultured in a carbon dioxide medium. 24 hours. Remove these supernatants, and check the increase or decrease of procollagen with the collagen prototype (I) ELISA kit (procollagen type (I); # MK101, TAKARA (Shiga, Japan)). The results are shown in Table 14. The collagen synthesis performance is shown based on the non-treated group 100.

[TABLE 14] As can be seen from the results in Table 14 above, the ginsenoside Mc according to the present invention exhibits superiority to a level higher than TGF-β as a positive control group. Therefore, it is known that the ginsenoside Mc according to the present invention can increase the amount of collagen produced around the pores, thereby reducing the enlarged pores. 2. Pore Shrinking Effect In order to understand the pore shrinking effect of Formulation Example 1 and Comparative Formulation Example 1, it was evaluated as follows. Twenty males and females with enlarged pores were selected and divided into two groups of 10 each. The members of each group applied the nutrition cream of Formulation Example 1 and Comparative Formulation Example 1 on the face for 4 weeks each day. Regarding the judgment of the effect of pore shrinkage, the photos were taken by experts before and 4 weeks after the experiment, and evaluated visually by experts. The results are shown in Table 15 below (evaluation level: 0-no reduction was seen; 5-significantly reduced).

[Table 15] From the results in Table 15 above, it can be seen that Comparative Formulation Example 1 does not have a pore shrinkage effect, while Formulation Example 1 shows a significant pore shrinkage effect that can be seen with the naked eye, and thus it can be seen that according to the ginsenoside Mc of the present invention, The effect of reducing pore size is excellent.

[Experimental Example 13] Sebum secretion inhibition effect Skin oversecretion inhibition effect by 1.5α-reductase activity inhibition In order to confirm 5α-reductase activity inhibition effect, HEK293-5αR2 cells were measured by [ ¹⁴ C] Testosterone is converted to [ ¹⁴ C] dihydrotestosterone (DHT: dihydrotestosterone) ratio. P3 x FLAG-CMV-5αR2 was transfected into HEK293 cells, and placed in a 24-well plate at 2.5 x 105 cells per well and cultured (Park et al., 2003, JDS. Vol. 31, pp. 191- 98). The next day, it was replaced with a new medium with enzyme substrate and inhibitor. As the substrate of the culture medium, 0.05 μCi [ ¹⁴ C] testosterone (Amersham Pharmacia biotech, UK) was used. In order to confirm the degree of inhibition of 5α-reductase activity, ginsenoside Mc was added and cultured in a 37%, 5% carbon dioxide incubator for 2 hours. At this time, the group to which ginsenoside Mc was not added was used as a negative control group, and the group to which finasteride was added was used as a positive control group. Then, the medium was collected, and the steroid was extracted with 800 μl of ethyl acetate. The upper organic solvent layer was separated and dried, and the remaining residue was dissolved again with 50 μl of ethyl acetate, and was dissolved in a silicone plastic sheet Kieselgel 60 F254. F254), ethyl acetate-hexane (1: 1) was used as a solvent for development. After drying the plastic sample in the air, use a bathing system to determine the amount of isotopic elements. Put the dried plastic piece and X-ray film together in the bath cassette. After 1 week, measure the testosterone and The isotope content of dihydrotestosterone was calculated based on the following formulas 5 and 6, respectively, and the conversion and inhibition rates were calculated. The results are shown in Table 16 below.

[Formula 5] Conversion rate (%) = radioactive energy in the DHT area / total radioactive energy × 100

[Formula 6] Inhibition rate (%) = (conversion rate of control group-conversion rate of test substance) / conversion rate of control group × 100

[TABLE 16] From the results in Table 16 above, it can be seen that ginsenoside Mc effectively inhibits the activity of 5α-reductase, which converts testosterone to dihydrotestosterone, combines it with water-soluble proteins in the cytoplasm, enters the nucleus and Activates sebaceous gland cells, promotes differentiation, and acts as a supersecretory sebum in the sebaceous glands. Based on this, it can be seen that ginsenoside Mc blocks the conversion from testosterone to dihydrotestosterone, which shows a higher effect than that used to inhibit the activity of 5α-reductase. Nastelier has more excellent suppression effect. Therefore, it can be seen that ginsenoside Mc inhibits the secretion of sebum by effectively inhibiting the activity of 5α-reductase. 2. Sebum secretion inhibiting effect In order to understand the sebum secretion inhibiting effect of the above-mentioned formulation example 1 and comparative formulation example 1, the evaluation was performed in the following manner. A total of 30 male and female subjects who were considered to have high sebum secretion were selected, and the nutrient creams of Formulation Example 1 and Comparative Formulation Example 1 were applied to a specified part of the facial skin, and applied for 4 weeks each day. Regarding the determination of the effect of sebum reduction, the average sebum reduction rate (%) after 2 weeks and 4 weeks was measured with a sebum volume measuring instrument (Sebumeter SM810, C + K Electronic Co., Germany), and the results are shown in Table 17 below.

[TABLE 17] From the results of Table 17 above, it can be seen that the dosage form example 1 containing the ginsenoside Mc of the present invention as an active ingredient can effectively suppress the excessive secretion of sebum compared to the comparative dosage form example 1 which does not contain it.

[Formulation example 3 and comparative formulation examples 3 to 4] Formulation example 3 and comparative formulation examples 3 to 4 were prepared based on the components and contents (% by weight) shown in Table 18 below. Specifically, ginsenoside Mc was added to formulation example 3, and comparative formulation example 3 did not contain effective ingredients for improving acne skin at all. Comparative formulation example 4 is a standard substance related to antibacterial efficacy, and it is mostly used for acne treatment. Erythromycin. Preparation methods of Formulation Example 3 and Comparative Formulation Examples 3 to 4 are as follows. The components of A in Table 18 below were completely dissolved, and the components of B were completely dissolved in a separate dissolution tank. B was added to A and mixed and dissolved. Among them, the component C was added according to the mixing ratio shown in Table 18, and the components were mixed after homogeneous filtration to prepare the present composition.

[TABLE 18]

[Test Example 14] Antibacterial effect test on acne By using each of the cosmetic compositions prepared with the composition of the above-mentioned dosage form example 3 and comparative dosage forms examples 3 to 4, a propionibacterium acnes (Propionibacterium acnes, P .acnes) (ATCC 6919: Medium-BHI Medium (broth)) was tested for antimicrobial activity. The acne-related antibacterial test method is shown below. (1) Preparation of test bacterial solution Propionibacterium acnes uses a culture solution inoculated in BHI medium and anaerobic cultured. (2) Preparation of diluted solution Add 0.15ml of the above test bacterial solution to 15ml of BHI medium (pH 6.8) or LB medium (pH 4.5), mix uniformly, and use it as a diluted solution. (3) Preparation of sample The cosmetic composition stock solutions prepared in dosage form example 3 and comparative dosage form examples 3 to 4 were used as samples directly. (4) Antibacterial test 1) Add the sample to the 96-well cell culture tube (96 well plate) line 1 at the initial concentration as a diluted solution and add the total amount in 200μl units. 2) After uniformly mixing the mixed solution in line 1 and taking 100 μl and placing it in line 2, uniformly mix, extract 100 μl again and put in line 3, and perform double dilution. 3) After performing incubation at 32 ° C for 24 hours and 48 hours, determine the proliferation of bacteria by the degree of suspension, and use the minimum concentration without bacteria proliferation as the minimum inhibitory concentration (MIC) value. If the mixed solution is opaque and it is difficult to judge the proliferation of the bacteria, it is confirmed by microscopic observation. The results of the acne-related antibacterial test are shown in Table 19. The minimum inhibitory concentration is marked as the concentration of the active ingredient contained in the dosage form.

[TABLE 19] From the results in Table 19 above, it can be seen that the smaller the ppm concentration of the lowest inhibitory concentration, the more effective it is in acne-related antibacterial properties. Formulation Example 3 is compared with the use of erythromycin, which is a known acne treatment agent. In Comparative Formulation Example 4, the ppm concentration was significantly small, and it was found that the composition containing ginsenoside Mc had a significantly superior antibacterial effect on the test bacteria.

[Experiment Example 15] Lipogenesis inhibition test 3T3-L1 cells of mouse fibroblast cell line were attached to a 10 × fetal calf containing 1 × 105 cells / well A 6-well culture plate of Dulbecos modified eagles medium (GIBCO BRL, Life Technologes) of fetal bovine serum (FBS). After 2 days, the medium was replaced with a new Dürber Co. modified Eagle's medium (containing 10% FBS), and the culture was continued for 2 days. Then, the above-mentioned cells that had been cultured were differentiated with a Dulberco's modified Eagle's medium (containing 10% FBS) containing 1 μg / ml insulin, 0.5 mM IBMX, and 0.25 μM dexamethasone. Induction and treatment of ginsenoside Mc and caffeine 50 μM, after 2 days of treatment, it was replaced again with Dürber's modified Eagle's medium containing insulin, and culture was continued for 5 days. After 5 days, the medium was replaced with normal medium (Durberco's modified Eagle's medium containing 10% FBS), and cultured while observing, until the above cells changed into morphologically fat cells. In order to evaluate the inhibitory effect of ginsenoside Mc on fat accumulation in adipocytes, Sudan III staining (S4136, sigma-aldrich) was performed on the differentiated 3T3-L1 adipocytes in the above process. The fat cells were fixed in phosphate buffered saline with 4% paraformaldehyde (pH 7.2) at normal temperature, washed with phosphate buffered saline (PBS, phosphate buffered saline), and then stained with Sudan III. Make a visual comparison. The control group only used the medium without adding the test substance or the comparative substance. As the other comparison group, the caffeine was treated at 50 μM. Regarding the degree of fat accumulation inhibition, the degree of staining is classified by dividing the degree of staining into +++, ++, +, and-. In this case, the closer to +++, the higher the degree of staining. The results are shown in Table 20 below.

[TABLE 20] From the results in Table 20 above, it can be seen that according to the ginsenoside Mc of the present invention, not only the amount of fat accumulated in fat cells is small, but also it has better lipid synthesis than caffeine, which is known as a lipid synthesis inhibitor. Inhibitory effect. Therefore, by inhibiting lipid synthesis and reducing sebum, the generation of acne can be suppressed.

[Experimental Example 16] Tests on the improvement of acne and the reduction of sebum secretion and the presence or absence of irritation The 30 subjects with acne were divided into 3 groups of 10 groups each, and each group of subjects was arranged to use the above dosage form The cosmetic composition prepared in Example 3 and Comparative Formulation Examples 3 to 4. The acne improvement scale is set from 1 to 5 points, 1 being "not", 3 being "fair", and 5 being "very yes". The experimental results are marked by the average scores of the 10 participants in Table 21 below. The period of acne disappearance is based on the number of days when the acne disappearance is read. The occurrence of acne recurrence is based on the result after one month. Sebum secretion reduction scale is set to 1 to 5 points, 1 is "not", 3 is "fair", and 5 is "very yes". The experimental results are marked by the average scores of the 10 participants in Table 21 below. The presence or absence of skin irritation is indicated by (the number of people who show a stimulus response) / (the total number of participants).

[TABLE 21] As can be seen from the results in Table 21 above, compared with Comparative Formulation Example 3, Formulation Example 3 has no recurrence of acne, and has an excellent effect on improving acne as a whole. In addition, in Comparative Formulation Example 4 containing an antibacterial standard substance, although it showed an acne-improving effect, it showed strong skin irritation during use, which is not suitable for long-term use. However, according to the composition of the present invention, Non-irritating, showing long-term use.

[Experimental Example 17] Inflammation improvement effect-IL-8 production inhibitory effect One day before the start of the experiment, normal human skin keratinocytes (NHEK, purchased: Lonza) were dispersed in 96-well plates at 5x104 cells / well Thereafter, the cells were cultured in a 5% carbon dioxide incubator at 37 ° C for 24 hours. After 24 hours, cells were washed twice with sulfate buffer (PBS) and replaced with serum-free KBM (serum-free keratinocyte basement media). To each well, ginsenoside Mc was treated at a concentration of 5 ppm, 25 ppm, and 50 ppm. After 30 minutes of reaction, PGSA (10 μg / ml), PGSA (50 μg / ml), PGSA (50 μg / ml) + LPS (1 μg / ml) ). Among them, PGSA (peptidoglycan from S. aureus) is a peptidoglycan extracted from Staphylococcus. It is the main component of the cell wall of Gram-positive (+) bacteria. The cell membrane component of bacteria can cause inflammation, especially According to reports, about 90% of patients with specific responsiveness have secondary infections caused by staphylococci. Endotoxin (LPS, lypopolysaccaride) is the main component of the cell membrane of Gram-negative (-) bacteria, which is the main cause of inflammation. After incubating in a 37 ° C, 5% carbon dioxide (CO2) incubator for 24 hours, the culture fluid was extracted, and an enzyme-linked immunosorbent assay (ELISA) was performed against Interleukin-8 (IL-8). The results are shown in Table 22. The enzyme-linked immunosorbent assay (ELISA) uses an experimental method of a manufacturing company (BD science).

[TABLE 22] As can be seen from the results in Table 22 above, ginsenoside Mc can significantly reduce and inhibit the secretion of IL-8 increased by PGSA and LPS. Therefore, the skin external preparation composition of the present invention can provide an excellent anti-inflammatory effect by significantly reducing the secretion of IL-8 increased by PGSA and LPS.

[Experimental Example 18] Evaluation of Antipruritic One day before the start of the experiment, keratinocytes (cell line name: HaCaT source: ATCC) were dispersed in 96-well plates with 4x104 cells / well, and incubated at 37 ° C and 5% carbon dioxide ) For 24 hours. After 24 hours, wash the 96-well plate twice with Hanks' Balanced Salt solution buffer, and then the reaction buffer (2μM Fluo-4-AM, 20% pluronic acid, 2.5mM probenecid) cells. Reaction in a 37 ° C, 5% carbon dioxide incubator for 30 minutes, and then at room temperature for 30 minutes, and then washed twice with Hank's balanced salt buffer solution at 0.05%, 0.1%, 0.5%, and 1.0% concentrations, respectively. (Wt%) ginsenoside Mc treated cells. After 10 minutes of reaction, 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) was treated, and the intracellular Ca2 + concentration was measured for 80 seconds. For measurement of changes in intracellular Ca2 + concentration, a multifunctional microplate reader 3 (FlexStation3: Molecular Device, USA) was used. After processing ginsenoside Mc and 2U / ml trypsin or 5μM PAR-2 active peptide (SLIGKV), measure Flex for 80 seconds, find the difference between the minimum and maximum values obtained, and compare it with 2U / The difference between the minimum value and the maximum value when treated with ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) was compared, and the inhibition rate (%) of intracellular influx of calcium ions is shown in Table 23 below.

[TABLE 23] From the results in Table 23 above, it can be seen that the amount of calcium ions flowing into the cells of trypsin or PAR-2 active peptide (SLIGKV) decreases with the treatment of ginsenoside Mc, and by increasing the concentration of ginsenoside Mc, Significantly reduces the influx of calcium ions. Therefore, the skin external preparation composition containing the ginsenoside Mc of the present invention can provide an excellent antipruritic effect by effectively inhibiting the PAR-2 activity that causes itching.

[Formulation Example 4 and Comparative Formulation Example 5] A shampoo was prepared with the composition shown in Table 24 below. Specifically, add surfactant and ethylene glycol distearate to purified water, heat to 80 ° C and dissolve uniformly, stir and gradually reduce the temperature to 40 ° C. After the effective ingredients and preservatives, viscosity modifiers, perfumes, and hair conditioners of the present invention, they are stirred and cooled to room temperature to complete the preparation.

[TABLE 24]

[Experimental Example 19] Anti-dandruff effect test 24 males aged 19-35 years old who had more dandruff were selected and divided into 2 groups with 12 in each group, and each group was arranged to use dosage form example 4 and comparative dosage form example 5 After using the shampoo for 1 month, the dandruff reduction rate was measured. Before starting the test, use a normal shampoo to shampoo, collect the dandruff accumulated within 2 days after shampooing, measure the weight of the dandruff, and then use the shampoos of Formulation Example 4 and Comparative Formulation Example 5 every two times. Shampoo once a day, collect dandruff accumulated within 2 days after the test is completed, measure the weight of dandruff, and compare the two. At this time, the accumulated dandruff was directly collected from the scalp by the vacuum suction device, and the dandruff reduction rate was obtained according to the following Equation 7. The results are shown in Table 25 below.

[Formula 7] Dandruff reduction rate (%) = {dandruff weight before the start of the test (mg)-dandruff weight after one month (mg)} / dandruff weight before the test (mg) × 100

[TABLE 25] As can be seen from the results in Table 25 above, Formulation Example 4 containing ginsenoside Mc exhibited an excellent antidandruff effect.

[Experimental Example 20] A total of 24 males and females aged 25 to 45 years with severe scalp pruritus were selected for the test for preventing scalp pruritus, and each group was divided into 2 groups with 10 members. The shampoo of Formulation Example 5 was used once every 3 days for 2 weeks, and the scalp itching prevention effect was evaluated by the following evaluation criteria. The results are shown in Table 26. [Evaluation criteria] Very good-5 points Excellent-4 points Fair-3 points Unqualified-2 points Very poor-1 point

[TABLE 26] From the results in Table 26 above, it can be seen that Formulation Example 4 containing ginsenoside Mc shows a more excellent effect in preventing itching of the scalp.

[Experimental Example 21] Evaluation of the Effect of Increasing the Activity of Potassium Channels Minoxidil as a hair loss treatment agent is considered to be a potential mitochondrial potassium channel opener (K _ATP channel opener), which is used for androgens Representative drug for sexual alopecia. In order to evaluate the mechanism of such minoxidil, a test method was used in which tolbutamide (SIGMA AlDRICH, T0891) for blocking mitochondrial potassium ion channels was treated in fibroblasts constituting the dermis of the scalp, and Inhibit cell proliferation, reopen potassium channels and restore cell proliferation. In order to evaluate the function of the composition as a mitochondrial potassium ion channel opener, a fibroblast cell line NIH3T3 (Mouse embryonic fibroblast cell line) cell line is used in the present invention. This cell line will be a natural immortal cell line derived from the fibroblast cell line of the NIH Swiss mouse embryo under the 3T3 protocol. The above cell lines were cultured in a Dulberco modified Iger medium (Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS in an incubator maintained at 5% carbon dioxide at 37 ° C for 24 hours. Put NIH3T3 into a 96-well plate, incubate in a 37 ° C incubator for 24 hours, and then process 2.5 mM tolbutamide. After 10 minutes, 10 μM minoxidil and ginsenoside Mc as positive control groups were 2.5 ppm , 5 ppm and 10 ppm treatment, 48 hours after drug treatment, WST-1 kit (Roche) was used to determine the cell proliferation ability. The results are shown in Table 27 below.

[TABLE 27] From the results in Table 27 above, it can be known that if ginsenoside Mc is treated, the proliferation of fibroblasts is restored. As the concentration of treated ginsenoside Mc increases, its cell proliferation capacity also increases. If ginsenoside Mc is treated at 10 ppm, Then, the degree of cell proliferation recovery is as high as that in the case of treating minoxidil.

[Experimental Example 22] Test for promoting melanin production by ginsenoside Mc Add 5% fetal bovine serum, 100IU penicillin G, and 0.2μM TPA to RPMI medium, and melanin cells (melan-a) are applied to a 24-well plate (24- well microtiter plate) was dispersed at 50,000 cells / well. The next day, the dispersed cells were treated with ginsenoside Mc as a test substance at a final concentration of 10 ppm or 50 ppm, 0.1% DMSO as a negative control group, and 100 μM IBMX as a positive control group, and then cultured at 37 ° C for 3 days. . After the culture was completed, the wells were washed with phosphate buffered saline (PBS), and 1N sodium hydroxide (NaOH) was added in an amount of 100 μl each time to dissolve the melanin in the cells. The absorbance of the dissolved melanin was measured at 405 nm using a microplate reader (Synergy2, BioTek (VT, USA)). The melanin production-promoting effect of ginsenoside Mc compared with the control group is shown in Table 28 below.

[TABLE 28] As can be seen from the results in Table 28 above, ginsenoside Mc promotes melanin synthesis in melanocytes and increases the amount of melanin production, thereby exhibiting an excellent melanin production promotion effect.

[Experimental Example 23] The expression promotion effect of small eye malformation-related transcription factor (MITF) and tyrosinase in melanocytes of ginsenoside Mc was promoted to a 6-well plate (6-well) through a 501mel cell line microtiter plate) were dispersed at 500,000 cells / well. For each well, 0.1% DMSO was treated as a negative control group, IBMX 100 μM was treated as a positive control group, and ginsenoside Mc was treated as a test group at 10 ppm, and cultured at 37 ° C. Protein was obtained after 24 hours, 48 hours, and 72 hours. The protein thus obtained was subjected to immunoblotting with small eye malformation-related transcription factor (MITF) and tyrosinase antibody. Protein extraction and western blotting typically use standard methods employed by those skilled in the art. After the immunoblot was completed, the results were compared with 100 of the negative control group, and the results are shown in Table 29 below.

[TABLE 29] From the results in Table 29 above, it can be seen that ginsenoside Mc can improve the expression of small eye malformation-related transcription factor (MITF) and tyrosinase protein in melanocytes.

[Test Example 24] Evaluation of antibacterial power of ginsenoside Mc An antibacterial test was performed to evaluate the antibacterial power of ginsenoside Mc. The specific method is described below. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa strains used for the experiments were cultured in Tryptic Soy Broth and Candida albicans 2. Aspergillus niger strains were cultured in Sabouraud Dextrose Broth. The culture solution was diluted 1/100 (1/10 of Candida albicans strain) to each culture medium and used as a test bacterial solution. Aspergillus niger was used as a test bacterial solution in a spore suspension prepared at 2 × 108 cfu / ml. 0.15 ml of the above-mentioned test bacteria solution was added to each 15 ml of the culture medium, and they were uniformly mixed and used as a diluted solution. In row 96 of a 96 well plate (96 well plate), ginsenoside Mc10 ppm was added in 16 μl each time, and 184 μl of a diluted solution was placed in each. Put 100 μl of the diluted solution into each of the remaining wells. After uniformly mixing the mixed solution of line 1 and extracting 100 μl and placing it in line 2, after uniformly mixing, extracting 100 μl again and placing it in line 3, in this way, a 2-fold dilution was performed. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were cultured in a thermostatic bath at 32 ° C. Candida albicans and Aspergillus niger were cultured at 25 ° C. Cultivate in a constant temperature bath at ℃. After 48 hours, the presence or absence of bacterial proliferation and the degree of suspension were confirmed by a microscope, and the minimum inhibitory concentration (MIC) value was determined, and the results are shown in Table 30 below.

[TABLE 30] From the results in Table 30 above, it can be seen that ginsenoside Mc exhibits antibacterial power against various strains, and thus it can be predicted that ginsenoside Mc can function as a natural preservative or antibacterial agent in the composition.

no

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Claims (13)

A use of ginsenoside Mc as an active ingredient is for preparing a composition for increasing skin elasticity. A use of ginsenoside Mc as an active ingredient is for preparing a composition for improving skin wrinkles. A use of ginsenoside Mc as an active ingredient, which is used for preparing a skin moisturizing composition. A use of ginsenoside Mc as an active ingredient is for preparing a composition for strengthening the skin barrier function. A use of ginsenoside Mc as an active ingredient is for preparing a composition for inducing skin keratinocyte differentiation. A use of ginsenoside Mc as an active ingredient is used for preparing a composition providing antibacterial effect. A use of ginsenoside Mc as an active ingredient is used for preparing a composition for improving skin color and complexion. A use of ginsenoside Mc as an active ingredient is for preparing a composition for shrinking skin pores. A use of ginsenoside Mc as an active ingredient is for preparing a composition for regulating sebum. A use of ginsenoside Mc as an active ingredient is a composition for preparing skin whitening. A use of ginsenoside Mc as an active ingredient is for preparing a composition for promoting hair growth. A use of ginsenoside Mc as an active ingredient is for preparing a composition for preventing white hair. A use of ginsenoside Mc as an active ingredient is used for preparing a composition for providing antiseptic effect.