TW201521736A - External composition for skin containing ginsenoside Mc - Google Patents

Abstract

The disclosure relates to providing compositions including Ginsenoside MC and having effects of anti-aging, skin wrinkles relief, whitening, moisturizing improvement, acne and skin problem improvement, atopy syndrome relief, and skin astringent and pores astringent. The disclosure may also provide compositions having effects of complexion improvement, hair-growth improvement, grey hair prevention, anti-dandruff and antiseptic.

Description

Skin external preparation composition containing ginsenoside Mc

The present invention relates to an effect of providing anti-aging, improving skin wrinkles, whitening, moisturizing and anti-inflammatory, improving acne and skin problems, improving atopic symptoms, skin astringation and pore contraction by containing ginsenoside Mc. It also provides a composition for improving skin color, promoting hair growth, improving white hair, anti-dandruff and antiseptic effects.

The skin of human beings is the first layer of protective film of the human body. It functions to protect organs in the body in the stimulation of external environment such as changes in temperature and humidity, ultraviolet rays, and public nuisance substances. It experiences various internal and external changes with age. It is necessary to change. That is, in terms of internal factors, the secretion of various hormones used to regulate metabolism is reduced, the function of immune cells and the activity of cells are reduced, resulting in a decrease in the biosynthesis of immune proteins and human structural proteins required by the human body; Due to the destruction of the ozone layer, the amount of ultraviolet light reaching the surface of the sun's rays increases, and as the environmental pollution gradually increases, the free radicals and active oxygen increase, resulting in a decrease in skin thickness, an increase in wrinkles, and a decrease in elasticity. Causes skin to become dull, often with skin problems, increased chloasma and freckles, and old age Spots, darkness, darkening, etc., cause various changes.

In order to prevent changes in the skin state caused by the internal and external causes of the skin and to maintain a healthy skin condition, an attempt is made to add bioactive substances extracted from various known animals, plants, microorganisms, etc. to the cosmetics. Improve skin condition.

Ginsenoside Mc (Ginsenoside Mc) is a natural form of ginseng containing ginsenosides (Rb1, Rg1, Rd, Rb2, Rc, Rf) which are decomposed by digestive enzymes and intestinal microorganisms, and have strong anticancer activity. It is disclosed that Korean Patent No. 164266 describes a method for using it as an anticancer drug, and has not yet disclosed the effect of improving the overall skin condition of the composition containing ginsenoside Mc as an active ingredient, and promoting hair growth and whitening. Hair, anti-dandruff and anti-corrosion as the overall effect of the external preparation for skin.

In view of this, the present inventors have found that ginsenoside Mc can provide anti-aging, improve skin wrinkles, whitening, moisturizing and anti-inflammatory effects, improve acne and skin problems, improve the symptoms of atopic reactions, and provide skin color improving effect. The present invention has been completed by promoting hair growth, improving white hair, anti-dandruff and antiseptic effects.

Accordingly, an object of the present invention is to provide a skin external preparation composition which exhibits an improvement in the overall state of the skin, promotes hair growth, and improves white hair, anti-dandruff and antiseptic effects by containing ginsenoside Mc.

In order to achieve the above object, the present invention provides an anti-aging skin external preparation composition comprising ginsenoside Mc as an active ingredient.

Moreover, the present invention provides a skin external preparation composition for wrinkle improvement comprising ginsenoside Mc as an active ingredient.

Moreover, the present invention provides a moisturizing skin external preparation composition comprising ginsenoside Mc as an active ingredient.

Moreover, the present invention provides a skin external preparation composition for acne improvement comprising ginsenoside Mc as an active ingredient.

Moreover, the present invention provides a skin external preparation composition for improving the color and skin color of ginseng saponin Mc as an active ingredient.

Moreover, the present invention provides a composition for external use of skin for shrinkage of pores comprising ginsenoside Mc as an active ingredient.

Moreover, the present invention provides a skin external preparation composition for a specific reactive skin improvement containing ginsenoside Mc.

Moreover, the present invention provides an anti-inflammatory skin external preparation composition containing ginsenoside Mc.

Moreover, the present invention provides a whitening skin external preparation composition containing ginsenoside Mc.

Moreover, the present invention provides a hair growth promoting composition containing ginsenoside Mc.

Moreover, the present invention provides a composition for preventing white hair containing ginsenoside Mc.

Further, the present invention provides a composition for anti-dandruff containing ginsenoside Mc.

Moreover, the present invention provides a natural preservative combination containing ginsenoside Mc Things.

The composition of the present invention not only provides anti-aging, improves skin wrinkles, whitening, moisturizing effect and anti-inflammatory, improves acne and skin problems, improves the symptoms of atopic symptoms, skin convergence and pore contraction by containing ginsenoside Mc. The effect can also provide skin color improvement effect, promote hair growth, improve white hair, anti-dandruff and anti-corrosion effect.

The composition according to the present invention contains ginsenoside Mc as an active ingredient.

The ginsenoside Mc used in the present invention has the structure of the following Chemical Formula 1.

The ginsenoside Mc of the present invention can be extracted from plants, and can also be used in the field. For the synthesis of the disclosed method, it is also possible to use commercially available ginsenoside Mc. Further, ginsenoside Mc can be obtained from ginseng extract. At this time, the type of ginseng used is not particularly limited, and ginseng, red ginseng, white ginseng, taiji ginseng, and tail ginseng can be used. Further, the ginseng extract includes all the leaching liquid obtained by leaching and decocting from ginseng, the concentrate obtained by reconcentrating part or all of the leaching liquid, or by drying the above concentrate again. The body, decoction, tincture, fluid extract and chemical substances contained in ginseng, which also play a major role, include the plant itself. The extract can be taken from all parts of ginseng such as stems, roots, leaves, flowers and fruits. It is not limited to an extract of a specific part. Further, a method of extracting ginsenoside Mc from a ginseng extract can be carried out by a method disclosed.

Specifically, the above ginsenoside Mc can be isolated by extracting the ginseng extract from ginseng with water or an organic solvent by a method well known in the art. The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, diethyl ether, ethyl acetate, chloroform, and a mixed solvent of the organic solvent and water, and preferably 80% ethanol is used. At this time, preferably, the extraction temperature is from 10 ° C to 80 ° C, and extraction is possible for 3 hours to 24 hours. Exceeding the above extraction temperature and extraction time will result in a decrease in extraction efficiency or a change in composition.

Preferably, in the composition of the present invention, the content of the above ginsenoside Mc is from 0.001% by weight to 50% by weight based on the total weight of the composition. When the content of the above-mentioned active ingredient is less than 0.001% by weight, the potency and effect of the above components are weak, and if the content of the above-mentioned active ingredient exceeds 50% by weight, problems occur in skin safety or dosage form.

The composition of the present invention can be used as an anti-aging skin external preparation composition, and has an outstanding effect of increasing skin elasticity and improving wrinkles.

The composition of the present invention can be used as a skin external preparation for moisturizing, which can strengthen the skin barrier function and induce differentiation of skin keratinocytes. Thereby, it can be effectively used as a composition for external use of the skin, thereby preventing or improving skin dryness, atopic dermatitis, contact dermatitis or acne due to incomplete differentiation of the epidermis.

The composition of the present invention can be used as a composition for external application of acne for skin improvement, and has excellent antibacterial effect, particularly an antibacterial effect against acne-causing bacteria, and an anti-inflammatory effect.

The composition of the present invention can be used as a composition for external use of skin for improving color and skin tone, and is suitable for use in the skin, and by expanding the capillaries and promoting blood circulation to ensure smooth absorption of nutrients by the skin, thereby suppressing aging, and its color and Excellent skin tone improvement.

The composition of the present invention can be used as a skin external preparation for improving pore contraction, sebum regulation and skin problems, and is suitable for use in the skin, suppressing sebum secreted by excess, promoting elimination of active oxygen and synthesis of collagen, Thereby, the pores are shrunk, and the effect of suppressing the skin problem by the reduction of the expression of the inflammatory factor is outstanding.

The composition of the present invention can be used as a composition for ameliorating skin-ameliorating, which not only provides excellent activity by suppressing the activity of Protease-Activated Receptor-2 (PAR-2) which induces itching. The anti-itch effect also provides an anti-inflammatory effect by reducing the secretion of interleukin-8 (IL-8). Therefore, the above ginsenoside Mc of the present invention can be suitably used as an active ingredient of a skin external preparation composition for stabilizing sensitive, irritating or atopic skin and scalp and for improving or alleviating pruritus, fever and inflammation. use.

The composition of the present invention can be used as a whitening composition which provides an excellent whitening effect by blocking tyrosinase activity and suppressing the production of melanin.

The composition of the present invention can be used as a hair growth promoting composition, which promotes hair growth and new hair generation by promoting a rest period hair cycle into a growing hair cycle, and also ensures healthy hair growth and prevention. And to suppress the state of finding elephants or hair thinning and thinning.

The composition of the present invention can be used as a composition for preventing white hair, which activates melanocytes and promotes melanin synthesis by increasing the expression of a small eye deformity-related transcription factor (MITF) of melanocytes, thereby providing advance Prevents the formation of white hair and promotes the growth of black hair.

The composition of the present invention can be used as an anti-dandruff skin external preparation composition, which effectively excretes toxins accumulated in the hair and scalp, purifies the scalp, and suppresses the proliferation and growth of dandruff bacteria, thereby preventing scalp inflammatory reaction. Moreover, it has an outstanding anti-oxidation effect against the formation and action of active oxygen, thereby providing an effect of soothing and strengthening the scalp and strengthening the inherent defense ability.

The composition of the present invention can be used as a natural preservative composition, which contains a natural ingredient, which has an excellent antiseptic effect and provides a harmless effect to the human body.

Compositions in accordance with the present invention may be formulated by a medium or mechanism that is permissible by cosmetic or dermatological means. It is suitable for all dosage forms suitable for topical application, for example, an emulsion, a suspension, a microemulsion, a microcapsule, a fine particle ball which can be obtained by dissolving a solution, a gel, a solid, a stirred anhydrous product, and dispersing a milk in a water form. Or in the form of ionic (liposomes) and nonionic vesicular dispersing agents, or in the form of creams, lotions, lotions, powders, ointments, sprays or concealers. provide. Or can also use foam It is used in the form of (Foam) or in the form of an aerosol composition containing a compressed propellant. These compositions can be prepared in the usual manner in the art.

In particular, the skin external preparation composition of the present invention can be formulated into a composition for scalp and hair when used as an anti-dandruff, hair growth or white hair prevention use, and the dosage form is not particularly limited, for example, a dosage form It is a dual-purpose care cream for hair conditioner, hair nutrient water, softener, hair cream, shampoo, hair lotion, hair lotion or scalp hair.

Further, the composition according to the present invention may contain a fatty substance, an organic solvent, a dissolving agent, a concentrate, a gel, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, and a surface. Active agents, water, ionic or nonionic emulsifiers, fillers, metal ion chelating agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilicity Or an emollient active agent, a lipid vesicular or an adjuvant commonly used in the field of cosmetic or dermatological sciences, as is conventionally used in cosmetics. The amount of the above adjuvant used is based on the amount generally used in the field of cosmetic or dermatological science.

Further, the composition of the present invention may contain a skin absorption promoting substance in order to increase the skin-improving effect.

Hereinafter, the structure and effects of the present invention will be more specifically described by way of test examples and dosage forms. However, the test examples and the formulation examples are provided for the purpose of facilitating the understanding of the present invention, and the scope and scope of the present invention are not limited to the following examples.

[Reference Example 1] Preparation of ginsenoside Mc

The ginsenoside Mc used to test the efficacy of the composition of the present invention was purchased from the AMBO Institute.

[Test Example 1] Elastase activity inhibition potency

The elastase activity inhibiting ability of ginsenoside Mc was determined by comparison with epigallocatechin gallate (EGCG). The elastase and matrix used were commercially purchased from Sigma-Aldrich (Cat. No. E0127).

The elastase activity inhibition was tested by the following test method.

In a 96-well plate, 200 μl of ginsenoside Mc and 50 μL of 20 μg/mL elastase. The type III solution was mixed in 10 mg/L Tris-HCl buffer (pH 8.0). Epigallocatechin gallate (EGCG) 250 μM was used as a positive control group, and distilled water was used as a non-treated group of the negative control group. Then, 100 μL of 0.4514 mg/mL N-SUCCINYL-ALA-ALA-ALA-p-NITROANILIDE formulated with the above buffer was added, and the mixture was reacted at 25 ° C for 15 minutes. After the end of the reaction, the absorbance at a wavelength of 415 nm (Synergy 2, BioTek (VT, USA)) was measured. A blank test was performed by the same method to make corrections.

The calculation method for the inhibition of elastase activity is shown in the following formula 1, and the results are shown in Table 1 below.

[Formula 1] Elastase activity inhibition rate (%) = {1 - (C - D) / (A - B)} × 100

A: Absorbance at a wavelength of 415 nm in the absence of addition of the test substance and enzyme addition

B: Absorbance at a wavelength of 415 nm in the absence of addition of the test substance and no addition of the enzyme

C: absorbance at a wavelength of 415 nm in the addition of the test substance, enzyme addition

D: absorbance at a wavelength of 415 nm in the addition of the test substance and no addition of the enzyme

From the results of Table 1 above, it is understood that the degree of inhibition of elastase activity of ginsenoside Mc is significantly better than that of epigallocatechin gallate (EGCG) which is an inhibitor of elastase activity, thereby confirming this. The ginsenoside Mc of the invention has excellent elastase activity inhibitory effect.

[Test Example 2] Collagenase (MMP-1) inhibitory ability

The collagenase production inhibitory ability of the ginsenoside Mc of the present invention is determined by comparison with Retinoic acid.

Human fibroblasts were added to a 96-well microtiter plate in a Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal bovine serum. In order to reach 5,000 cells/well, it was cultured in a 5% carbon dioxide, 37 ° C incubator until it grew by about 70-80%. The ginsenoside Mc or retinoic acid was treated at a concentration of 10 μg/ml for 24 hours, and then the cell culture solution was extracted.

The degree of collagenase production of the extracted cell culture medium was measured by a commercially available collagenase measuring instrument (American Amway Biotech Co., Inc., Catalog #: RPN 2610). First, the extracted cell culture solution was placed in a 96-well plate, which was uniformly coated with a collagenase antibody, and subjected to an antigen-antibody reaction for 3 hours in a thermostat. After 3 hours, the chromogenic group will be combined The secondary collagen antibody was added to a 96-well plate and reacted again for 15 minutes. After 15 minutes, the coloring inducing substance (3,3',5,5'tetramethylbenzidine, sigma) was added, and the color was induced for 15 minutes at room temperature, and 1 M sulfuric acid was added again to stop the color reaction, and the color of the reaction liquid It is yellow, depending on the degree of reaction, the degree of yellow display varies.

The absorbance of a yellow 96-well plate was measured at 405 nm by a light absorption meter, and the degree of synthesis of collagenase was calculated by the following formula 2, and the results are shown in Table 2. At this time, the reaction absorbance of the cell culture solution extracted from the group which did not treat the composition was used as a control group.

[Formula 2] Degree of expression of collagenase (%) = absorbance of substance-treated cell group / absorbance of control group × 100

From the results of the above Table 2, it was found that the degree of collagenase expression of ginsenoside Mc was similar to that of the collagenase (Retinoic acid) which is an inhibitor of collagenase expression, similarly to the inhibitory effect on collagenase expression.

From such a result, it was confirmed that the ginsenoside Mc of the present invention has an effect of inhibiting matrix metalloproteinase (MMP-1).

[Formulation Example 1 and Comparative Formulation Example 1]

According to the composition of Table 3 below, the nutrient cream is prepared by the usual method (unit: weight%).

[Test Example 3] Confirmation of skin elasticity enhancing performance

In order to confirm the skin elasticity increasing effect against the human body, the following Formulation Example 1 and Comparative Formulation Example 1 were evaluated as follows.

Twenty healthy women in the age group of 30 to 40 years old were divided into two groups in the group of 10, which corresponded to the two groups of the dosage form example 1 and the comparative dosage form, respectively, and the nutrition cream was applied to the subjects. The skin of the face was measured once a day for 12 weeks, and the skin elasticity was measured by a skin elasticity measuring instrument (Cutometer SEM 575, C+K Electronic Co., Germany). The results are shown in Table 4 below. The results of Table 4 are reported as the ΔR8 value of Cutometer SEM 575, while the R8 value indicates the nature of skin viscoelasticity.

From the results of the above Table 4, it was found that the dosage form 1 containing the ginsenoside Mc of the present invention further increased the skin elasticity compared to the group of the comparative comparative dosage form 1.

Thus, it was confirmed that the composition containing the ginsenoside Mc of the present invention is very effective for increasing skin elasticity.

[Test Example 4] Confirmation of skin wrinkle improvement performance

In order to confirm the wrinkle-improving effect of the composition of the present invention, the above-mentioned Formulation Example 1 and Comparative Formulation Example 1 were used.

In order to confirm the wrinkle improving effect of the above Formulation Example 1 and Comparative Formulation Example 1, evaluation was carried out as follows. Twenty healthy women in the 40-year-old age group were divided into two groups in the group of 10, which corresponded to the two groups of the dosage form example 1 and the comparative dosage form, respectively, and the nutrient cream was applied to the face of the subject. The replica was extracted by gelatin once a day for 12 consecutive weeks, and was measured by a skin measuring instrument (visiometer, SV600, Courage+Khazaka electronic GmbH, Germany) and subjected to image analysis. The results are shown in Table 5 below. The values in Table 5 below represent the average of the values of the parameters before the smearing were removed from the parameter values after 12 weeks of application.

From the results of the above Table 5, it was found that the agent composition for the formulation of Example 1 had excellent skin wrinkle improvement effect.

[Test Example 5] Tyrosinase inhibitory effect

Tyrosinase was extracted from the mushroom using tyrosinase prepared by SIGMA. First, a tyrosine as a substrate was dissolved in distilled water to prepare a solution of 0.3 mg/ml, and the solution was added to a test tube in an amount of 1.0 ml, respectively, and then 1.0 ml of a potassium-phosphate buffer solution (0.1 mol concentration) was added thereto. , pH 6.8) and 0.7 ml of distilled water.

0.2 ml of a sample liquid of ginsenoside Mc prepared by mixing at 10 ppm was added to the ethanol reaction solution of the present invention, and reacted in a thermostat at 37 ° C for 10 minutes. At this time, 0.2 ml of the solvent was placed in place of each sample solution, and the mixture was placed in a control group, and ascorbic acid was used as a positive control group. To the reaction solution, 2500 units/ml of tyrosinase solution was added in an amount of 0.1 ml each time, and reacted again in a thermostat at 37 ° C for 10 minutes. The test tube to which the reaction solution was added was placed in ice water, rapidly cooled and the reaction was stopped, and the absorbance at a wavelength of 475 nm (Synergy 2, BioTek (VT, USA)) was measured by a photoelectric spectrum analyzer, and the results are shown in the following table. 6. The respective tyrosinase inhibitory effects were calculated by the following formula 3.

[Formula 3] Tyrosinase inhibition rate (%) = 100 - (reaction absorbance of test substance / control group) Reaction absorbance) × 100

From the results of the above Table 6, the tyrosinase of the ginsenoside Mc according to the present invention has a much higher inhibition rate than the ascorbic acid which is known as a tyrosinase inhibitor, and thus the whitening effect is excellent.

[Test Example 6] Inhibition of melanin production by B16/F10 melanoma cells

A sample containing 0.001% by weight of each of ginsenoside Mc and ginic acid (Kojic acid) was used as a test substance, and this was added to a culture solution of B16/F10 melanoma cells (Korean cell line bank) at a predetermined concentration for 3 days. After the culture, the culture solution was removed, washed with phosphate buffered saline (PBS), and the cells were lysed with 1 N sodium hydroxide (NaOH), and the absorbance was measured at 405 nm (Synergy 2, BioTek (VT, USA)). The cells to which no test substance was added were used as a control group, and the degree of melanin production inhibition of each test substance was measured by comparison with the melanin content in the control group. The melanin production inhibition rate was calculated according to Formula 4, and the results are shown in Table 7.

[Formula 4] Melanin production inhibition rate (%) = 100 - (absorbance of test substance / absorbance of control group) × 100

From the results of the above Table 7, the ginsenoside Mc according to the present invention has a melanin production inhibition rate which is much higher than that of the known Kojic acid as a melanin production inhibitor, and it is found that the whitening effect is excellent.

[Test Example 7] Determination of skin moisturizing effect

In order to determine the effect of ginsenoside Mc on the skin moisturizing power, the above Formulation Example 1 and Comparative Formulation Example 1 were used and evaluated as follows.

Twenty adult males and females of the dry skin type of 40 to 50 years old were divided into two groups in the form of 10 in each group, which corresponded to the two groups of the dosage form example 1 and the comparative dosage form example 1, respectively, and the nutrient cream was added. Apply to the subject's face twice a day for 12 weeks. Before the start of application, after 1 week of application, after 2 weeks of application, after 4 weeks of application, and after 2 weeks of smearing (6 weeks in total), under constant temperature and humidity conditions (24 ° C, relative humidity 40%), by The skin moisture content was measured by a skin moisture measuring instrument (Corneometer CM825, C+K Electronic Co., Germany). The results are shown in Table 8 below. The results in Table 8 show the percentage of the increase in the measured value after the treatment for a predetermined period of time, based on the measured value of the skin moisture measured on the eve of the test.

From the above Table 8, when the comparative dosage form example 1 was applied, the fourth week of the application was cut off, which showed a moisture increase rate of about 30%, and after the application was stopped, In contrast, when the dosage form 1 containing ginsenoside Mc was applied, the skin moisture increase rate of most 30% or more was also exhibited after the application was stopped. Thereby, it is understood that the composition of the present invention containing ginsenoside Mc is excellent in skin moisturizing effect.

[Test Example 8] Determination of differentiation promoting effect of keratinocytes

In order to understand the effect of promoting differentiation of keratinocytes of ginsenoside Mc, the amount of CE (Cornified Envelop) generated at the time of differentiation of keratinocytes was measured by absorbance in the following manner. First, the keratinocytes of the human body which have been cultured once are placed in a culture flask and attached to the bottom, and the keratinocytes are separated from the epidermis of the newborn, and then the ginsenoside Mc is treated to a culture solution at a concentration of 5 ppm, and Incubate for 5 days until the cells grow to about 70-80% of the bottom area. At this time, the low calcium (0.03 mM) treatment group and the high calcium (1.2 mM) treatment group were used as a negative control group and a positive control group, respectively. Then, the above-cultured cells were extracted and washed with Phosphate buffered saline, and 1 ml of a 10 mM concentration of 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) was added. Sonication, boiling, and centrifugation were performed in hydroxymethylaminomethane hydrochloride buffer (Tris-HCl, pH 7.4), and the pellet was resuspended in 1 ml of phosphate buffered saline (PBS). Absorbance was measured at 340 nm (Synergy 2, BioTek (VT, USA)). Further, the above-mentioned solution after completion of the ultrasonic treatment was partially extracted, and the protein content thereof was measured, which was used as a reference for evaluating the degree of cell differentiation. The result shows In Table 9.

From the results of the above Table 9, it is understood that when ginsenoside Mc is used, the differentiation promoting effect of keratinocytes is excellent.

[Test Example 9] Determination of skin barrier function recovery effect

In order to determine the effect of ginsenoside Mc on the recovery of damaged skin barrier function due to skin injury, the following experiment was performed. The skin barrier damage was applied to 10 adult male and female upper arms by the Tape Stripping method, and the two groups of the medicated dosage form 2 and the comparative dosage form 2 were respectively prepared by the composition of the following Table 10, The degree of recovery from the epidermal water loss rate (TWEL) was measured and compared by Vapometer (Delfin, Finland) once a day for 7 consecutive days. Among them, Comparative Formulation Example 2 was used as a negative control group, which was a vehicle. The test results are shown in Table 11 below. The results in Table 11 compare the treatment differences before barrier damage and after barrier damage on a 100% basis.

From the results of Table 11 above, if the comparative dosage form 2 which does not contain ginsenoside Mc is treated, the transepidermal water loss rate gradually increases with time; on the contrary, if the dosage form containing ginsenoside Mc is treated, Then, the epidermal water loss rate quickly returns to normal, and the barrier damage is restored.

[Test Example 10] Gas color improvement effect

In order to evaluate the skin blood circulation promoting effect of the cosmetic composition according to the present invention, blood circulation of the skin was measured by a Laser Doppler Perfusion Imager (Periscan PIM II, Perimed (stochholm, Sweden)). degree. A laser Doppler blood flow imager is a commonly known instrument for measuring blood circulation in the skin, which is currently used to measure not only the speed and amount of blood in the capillaries of the skin, It can also measure the flow in small arteries and venules, which is a very sensitive device.

In a constant temperature and humidity chamber, after washing with a soap, relax for 30 minutes, and the initial value was measured by a laser Doppler blood flow imager. First, the initial blood flow of the lower part of the forehead was measured by a laser Doppler blood flow imager for 30 women who usually had cold hands and feet. Then, the subjects were arranged to use the above-mentioned Formulation Example 1 and Comparative Formulation Example 1 in one week, and the measured blood flow rate and the above-mentioned initial measurement values were compared, and the results (changes in skin blood flow rate) are shown in Table 12 below.

From the results of the above Table 12, the cosmetic composition according to the present invention significantly increased the skin blood flow rate compared to the comparative dosage form Example 1 which did not contain ginsenoside Mc, and it was found that the color of the blood was promoted by the promotion of such blood circulation. Improved. In summary, the results represent a cosmetic composition containing ginsenoside Mc according to the present invention, which contributes to the effective delivery of nutrients to the skin, inhibits and delays skin aging.

[Test Example 11] Skin color improvement effect

In order to understand the skin color improvement effect of the above Formulation Example 1 and Comparative Formulation Example 1, 30 subjects were arranged to use (1 time/day for 1 week) by Facial Stage DM-3 (Moritex, Japan) equipment. Assess the degree of skin color improvement. Regarding the skin color improvement rate, the skin color improvement rate was measured by measuring the brightness and color measurement value of the skin, and the results are shown in Table 13 below. The greater the brightness and color change value, the higher the skin color improvement.

As is apparent from the results of the above Table 13, the comparative dosage form 1 which does not contain the ginsenoside Mc according to the present invention, which does not exhibit significant skin color improving efficacy; on the contrary, the dosage form containing ginsenoside Mc as an active ingredient Compared with the skin color before use, the skin color after use is greatly improved.

[Test Example 12] Pore contraction effect

1. By promoting the pore shrinkage effect of collagen biosynthesis

The collagen biosynthesis promoting effect of ginsenoside Mc according to the present invention was determined by comparison with TGF-β. First, put fibroblasts Each well was seeded at 24 wells in a manner of 105 cells, and cultured until it grew to about 90%. The ginseng saponin Mc and TGF-β of the present invention dissolved in a serum-free medium were treated at 10 ng/ml in a serum-free Dulberaceae modified Eagle's medium for 24 hours, and cultured in a carbon dioxide medium at 10 ng/ml. 24 hours. The supernatant liquid was removed, and the increase or decrease of collagen procollagen was examined by a collagen prototype (I) ELISA kit (procollagen type (I); #MK101, TAKARA (Shiga, Japan)). The results are shown in Table 14, and the synthesis performance of collagen is shown on the basis of the non-treatment group 100.

As is apparent from the results of the above Table 14, the ginsenoside Mc according to the present invention exhibited superiority to the extent of TGF-? as a positive control group. Thus, it is understood that the ginsenoside Mc according to the present invention can increase the amount of collagen produced around the pores, thereby reducing the enlarged pores.

2. Pore contraction effect

In order to understand the pore shrinkage effect of Formulation Example 1 and Comparative Formulation Example 1, evaluation was carried out in the following manner. Twenty male and female subjects with enlarged pores were divided into two groups, each of which was divided into two groups. Each group member was applied with facial creaming agent type 1 and comparative dosage form example 1 for 4 weeks. Regarding the effect of the effect of the pore contraction, the naked eye was evaluated by an expert by photographs before the experiment and after 4 weeks of the experiment. The results are shown in Table 15 below (evaluation level: 0 - no reduction; 5 - sharp reduction).

From the results of the above Table 15, it was found that Comparative Formulation Example 1 did not have a pore-shrinking effect, and Formulation Example 1 exhibited a remarkable pore-shrinking effect which was visible to the naked eye, whereby it was found that ginsenoside Mc according to the present invention, The effect of reducing the pore size is excellent.

[Test Example 13] Sebum secretion inhibiting effect

Skin hypersecretion inhibitory effect inhibited by 1.5α-reductase activity

To confirm 5α- reductase (5 α -reductase) activity inhibition effect was measured in HEK293-5αR2 cells by ^[14 C] testosterone is converted to ^[14 C] dihydrotestosterone: ratio (DHT dihydrotestosterone) of. P3 x FLAG-CMV-5αR2 was transfected into HEK293 cells and plated in 24-well plates per well/2.5 x 105 cells (Park et al., 2003, JDS. Vol. 31, pp. 191- 98). The next day, the new medium was added to the enzyme matrix and inhibitor. 0.05 μCi of [ ¹⁴ C]testosterone (Amersham Pharmacia biotech, UK) was used as a substrate for the medium.

In order to confirm the degree of inhibition of 5α-reductase activity, ginsenoside Mc was added and cultured at 37 ° C for 2 hours in a 5% carbon dioxide incubator. At this time, the group in which no ginsenoside Mc was added was used as a negative control group, and the group of finasteride was added as a positive control group. Then, the medium was taken, the steroid was extracted with 800 μl of ethyl acetate, the upper organic solvent layer was separated and dried, and the remaining residue was again dissolved in 50 μl of ethyl acetate in a plastic sheet of 60 F254 (Silica plastic sheet kieselgel 60). On F254), ethyl acetate-hexane (1:1) was developed as a solvent.

After drying the plastic sample in the air, a bathing system was used to measure the amount of the ectopic element, and the dried plastic sheet and the X-ray film were placed together in a bath cassette, and the testosterone remaining on the film was measured after one week. The amount of the isotopic element of dihydrotestosterone was calculated from the following formula 5 and formula 6, and the conversion ratio and the inhibition ratio were respectively calculated. The results are shown in Table 16 below.

[Formula 5] Conversion rate (%) = radioactivity / total radioactivity in the DHT region × 100

[Formula 6] Inhibition rate (%) = (conversion rate of the control group - conversion rate of the test substance) / conversion ratio of the control group × 100

From the results of Table 16 above, it is known that ginsenoside Mc effectively inhibits the activity of 5α-reductase, which converts testosterone into dihydrotestosterone, binds it to the cytoplasmic water-soluble protein, and enters the nucleus. Activates sebaceous gland cells, promotes differentiation, and acts to secrete sebum in the sebaceous gland. Thus, it can be seen that ginsenoside Mc blocks the conversion from testosterone to dihydrotestosterone, which exhibits a lower activity than that used to inhibit 5α-reductase. That Stella has a better suppression effect. Therefore, it is understood that ginsenoside Mc inhibits the excessive secretion of sebum by effectively inhibiting the activity of 5α-reductase.

2. Sebum secretion inhibition effect

In order to understand the sebum secretion inhibiting effect of the above Formulation Example 1 and Comparative Formulation Example 1, evaluation was carried out in the following manner. A total of 30 male and female subjects with sebum secretion were selected, and the nutrient cream of the dosage form 1 and the comparative dosage form 1 was applied to the designated parts of the facial skin for 4 consecutive weeks. Regarding the determination of the effect of sebum reduction, the average sebum reduction rate (%) after 2 weeks and 4 weeks was measured by a sebum measuring instrument (Sebumeter SM810, C+K Electronic Co., Germany), and the results were shown in Table 17 below.

From the results of the above-mentioned Table 17, it is understood that the dosage form 1 which contains the ginsenoside Mc of the present invention as an active ingredient can effectively suppress excessive secretion of sebum as compared with the comparative dosage form 1 which does not contain it.

[Formulation Example 3 and Comparative Formulation Examples 3 to 4]

Formulation Example 3 and Comparative Formulation Examples 3 to 4 were prepared according to the components and contents (% by weight) shown in Table 18 below. Specifically, in Formulation Example 3, ginsenoside Mc was blended, and Comparative Formulation Example 3 contained no active ingredient for improving acne skin, and Comparative Formulation Example 4 was used as a reference material for antibacterial power, and it was used as a acne treatment. Erythromycin (erythromycin).

Formulation Example 3 and Comparative Formulation Examples 3 to 4 were prepared as follows. The components of A of Table 18 below were completely dissolved, and the component of B was completely dissolved in a separate dissolution tank, and B was added to A and mixed and dissolved. Here, the components of C were added in accordance with the ratio shown in Table 18, uniformly mixed, and then filtered to prepare the composition.

[Test Example 14] Antibacterial test for acne

Each of the cosmetic compositions prepared by the composition of the above-mentioned Formulation Example 3 and Comparative Formulation Examples 3 to 4, P. acnes as a source strain of acne (Propionibacterium acnes, P. acnes) (ATCC 6919: medium-BHI medium (broth)) was subjected to an antimicrobial test.

The test method for acne related antibacterial power is as follows.

(1) Test bacterial preparation

Propionibacterium acnes was cultured using anaerobic culture inoculated in BHI medium.

(2) Preparation of diluted solution

0.15 ml of the above test bacterial solution was added to 15 ml of BHI medium (pH 6.8) or LB medium (pH 4.5), and uniformly mixed, and used as a diluted solution.

(3) Sample preparation

The stock solution of the cosmetic composition prepared in Formulation Example 3 and Comparative Formulation Examples 3 to 4 was directly used as a sample.

(4) Antibacterial test

1) A 96-well cell culture tube (96 well plate) No. 1 was placed, and the sample was placed at a starting concentration, and as a diluted solution, the total amount was added in units of 200 μl.

2) After uniformly mixing the mixture of No. 1 line, 100 μl was taken and placed in line 2, uniformly mixed, 100 μl was again extracted and placed in row 3, and double dilution was performed.

3) After 24 hours and 48 hours of static culture at 32 ° C, the proliferation of the bacteria was judged by the degree of suspension, and the minimum concentration without bacterial growth was taken as the Minimum Inhibitory Concentration (MIC) value. If the mixed solution is opaque and it is difficult to judge whether or not the bacteria are proliferated, it is confirmed by microscopic observation.

The results of the acne-related antibacterial test are shown in Table 19. Minimum inhibitory concentration conversion The labeling is carried out by the concentration of the active ingredient contained in the dosage form.

From the results of the above Table 19, it is understood that the smaller the ppm concentration of the minimum inhibitory concentration, the more effective the substance can be used as a more effective substance in the acne-related antibacterial power, and the dosage form Example 3 is compared with the use of erythromycin as a known acne therapeutic agent. In Comparative Formulation Example 4, the ppm concentration was remarkably small, and it was found that the composition containing ginsenoside Mc had remarkably excellent antibacterial activity against the test bacteria.

[Test Example 15] Lipogenesis inhibition test

3T3-L1 cells of the fibroblast cell line of mice were attached to Dürber containing 10% fetal bovine serum (FBS) at 1×10 5 cells/well. A 6-well culture plate of the culture medium of Dulbecos modified eagles medium (GIBCO BRL, Life Technologes). After 2 days, the medium was replaced with a new Dulberaceae modified Eagle's medium (containing 10% FBS) and culture was continued for 2 days. Then, for the above-mentioned cells to be cultured, differentiation was carried out with Dulbecco's modified Eagle's medium (containing 10% FBS) containing 1 μg/ml insulin (insulin), 0.5 mM IBMX, and 0.25 μM dexamethasone (dexamethasone). After induction, the ginsenoside Mc and caffeine 50 μM were treated, and after 2 days of treatment, they were replaced with Dulberaceae modified Eagle's medium containing insulin, and culture was continued for 5 days. After 5 days, it was replaced with normal medium (Dulber's modified Eagle's medium containing 10% FBS) while observing one side. The cells are cultured until the above cells are changed in morphology to adipocytes.

In order to evaluate the inhibitory potency of ginsenoside Mc against fat accumulation in fat cells, Sudan III staining (S4136, sigma-aldrich) was carried out by performing differentiation of 3T3-L1 adipocytes in the above process. The fat cells were fixed in phosphate buffer at 4% polyformaldehyde (pH 7.2) at room temperature, washed with phosphate buffered saline (PBS), stained with Sudan III, and photographed. Compare with the naked eye. In the control group, only the medium to which the test substance or the comparative substance was not added was used, and as the other comparison group, the caffeine was treated at 50 μM. Regarding the degree of suppression of fat accumulation, the degree of dyeing is classified into +++, ++, +, -, and the level is given. In this case, the closer to +++, the higher the degree of dyeing. The results are shown in Table 20 below.

As is apparent from the results of the above Table 20, the ginsenoside Mc according to the present invention has not only a small amount of fat accumulated in the fat cells, but also has superior lipid synthesis as compared with caffeine which is known as a lipid synthesis inhibiting substance. Inhibitory effect. Therefore, sebum can be reduced by inhibiting lipid synthesis, thereby suppressing the production of acne.

[Test Example 16] Test for improvement of acne and reduction of sebum secretion and stimulation

Thirty subjects with acne were divided into three groups in the form of 10 persons in each group, and each group of the subjects was arranged to use the cosmetic compositions prepared in the above-mentioned Formulation Example 3 and Comparative Formulation Examples 3 to 4. The acne improvement scale is set to 1 to 5 points, 1 point "No", 3 is divided into "general", and 5 is classified as "very". Experimental Results The average scores of the 10 subjects in Table 21 below were marked.

The period of disappearance of acne is based on the number of days on which the read acne disappears, and whether the acne recurrence is based on the result one month later. The sebum secretion reduction scale is set to 1 to 5 points, 1 for "no", 3 for "general", and 5 for "very". Experimental Results The average scores of the 10 subjects in Table 21 below were marked. The presence or absence of skin irritation (the number of people who showed irritating response) / (the total number of subjects).

From the results of the above Table 21, it was found that the dosage form of Example 3 had no recurrence of acne as compared with Comparative Formulation Example 3, and as a whole, it had an excellent effect on the improvement of acne. Further, in Comparative Example 4 containing an antibacterial power standard substance, although exhibiting an acne-improving effect, it exhibits strong skin irritation during use, which is not suitable for long-term use, and according to the composition of the present invention, Non-irritating, showing long-term use.

[Test Example 17] Inflammatory improvement effect - IL-8 production inhibitory effect

One day before the start of the experiment, skin human keratinocytes (NHEK, purchased at: Lonza) were dispersed in a 96-well plate at 5x104 cells/well, and a 5% carbon dioxide incubator at 37 °C. Train for 24 hours. After 24 hours, the cells were washed twice with sulfate buffer (PBS) and replaced with serum-free KBM (serum-free keratinocyte medium, serum). Free keratinocyte basement media). The ginsenoside Mc was treated at a concentration of 5 ppm, 25 ppm, and 50 ppm to each well, and after 30 minutes of reaction, PGSA (10 μg/ml), PGSA (50 μg/ml), PGSA (50 μg/ml) + LPS (1 μg/ml) were separately treated. ). Among them, PGSA (peptidoglycan from S. aureus) is a peptidoglycan extracted from Staphylococcus aureus, which is a major component of the cell wall of Gram-positive (+) bacteria, and the cell membrane component of bacteria can cause inflammation, in particular, According to the report, about 90% of patients with atopic reactions cause secondary infections due to staphylococci. Endotoxin (LPS, lypopolysaccaride) is a major component of the cell membrane of Gram-negative (-) bacteria, which is the main cause of inflammation.

After culturing for 24 hours at 37 ° C in a 5% carbon dioxide (CO 2 ) incubator, the culture solution was extracted and an enzyme-linked immunosorbent assay (ELISA) was performed on Interleukin-8 (IL-8). The results are shown in Table 22. The enzyme-linked immunosorbent assay (ELISA) uses the experimental method of the manufacturing company (BD science).

As can be seen from the results of Table 22 above, ginsenoside Mc significantly reduced and inhibited the secretion of IL-8 which was increased by PGSA and LPS. Thus, the skin external preparation composition of the present invention significantly reduces IL-8 by PGSA and LPS by Secreted to provide excellent anti-inflammatory effects.

[Test Example 18] Antipruritic evaluation

One day before the start of the experiment, keratinocytes (cell line name: HaCaT source: ATCC) were dispersed in a 96-well plate at 4x104 cells/well, and cultured at 37 ° C in a 5% carbon dioxide incubator for 24 hours. After 24 hours, after washing twice with a Hanks Balanced Salt solution buffer, the reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM) was used. Probenecid) cells. The reaction was carried out in a 5% carbon dioxide incubator at 37 ° C for 30 minutes, and then reacted at room temperature for 30 minutes, and then washed twice with Hank's balanced salt buffer, respectively, at concentrations of 0.05%, 0.1%, 0.5%, and 1.0%. (% by weight) of ginsenoside Mc treated cells.

After 10 minutes of reaction, 2 U/ml trypsin (Trypsin) or 5 μM PAR-2 active peptide (SLIGKV) was treated, and the intracellular Ca2+ concentration was measured for 80 seconds. For the measurement of changes in intracellular Ca2+ concentration, a multi-function microplate reader 3 (FlexStation 3: Molecular Device, USA) was used. After treating ginsenoside Mc and 2 U/ml trypsin (Trypsin) or 5 μM PAR-2 active peptide (SLIGKV), Flex was measured for 80 seconds, and the difference between the obtained minimum and maximum values was determined and compared with 2 U/ The difference between the minimum value and the maximum value of the treatment of ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) was compared, and the intracellular influx inhibition rate (%) of calcium ions is shown in Table 23 below.

As can be seen from the results of Table 23 above, the amount of calcium ions which flow into the cells by trypsin or PAR-2 active peptide (SLIGKV) decreases with the treatment of ginsenoside Mc, and the concentration of ginsenoside Mc can be increased by increasing the concentration of ginsenoside Mc. Significantly reduce the influx of calcium ions.

Therefore, the skin external preparation composition containing the ginsenoside Mc of the present invention can provide an excellent antipruritic effect by effectively inhibiting the PAR-2 activity which causes itching.

[Formulation Example 4 and Comparative Formulation Example 5]

The shampoo was prepared as follows in the composition of Table 24. Specifically, a surfactant and ethylene glycol distearate are added to purified water, heated to 80 ° C and uniformly dissolved, stirred and gradually cooled to 40 ° C, and added to the above mixture. After the active ingredient of the present invention and a preservative, a viscosity modifier, a fragrance, and a hair conditioner, the mixture is stirred and cooled to room temperature to complete the preparation.

[Test Example 19] Anti-dandruff effect test

24 males aged 19-35 who had more dandruff were selected and divided into 2 groups in groups of 12, and each group was treated with the shampoo of Formulation Example 4 and Comparative Formulation Example 5 for one month. Thereafter, the rate of dandruff reduction was measured.

Before starting the test, use general shampoo shampoo, collect the dandruff accumulated within 2 days after shampooing, measure the weight of the dandruff, and then use the shampoo of Formulation Example 4 and Comparative Formulation Example 5, respectively. The hair was shampooed once a day, and the dandruff accumulated within 2 days after the completion of the test was collected, and the weight of the dandruff was measured, and the two were compared. At this time, the accumulated dandruff was directly collected from the scalp by a vacuum suction device, and the dandruff reduction rate was obtained according to the following formula 7, and the results are shown in Table 25 below.

[Formula 7] Dandruff reduction rate (%) = {head dandruff weight (mg) - dandruff weight (mg) after one month} / dandruff weight (mg) before test start × 10O

From the results of Table 25 above, it is known that the dosage form containing ginsenoside Mc is 4 Shows excellent anti-dandruff effect.

[Test Example 20] Test for prevention of scalp pruritus

A total of 24 males and females aged 25 to 45 years old with severe scalp pruritus were selected. Each group was divided into 2 groups in 10 groups. The shampoos of each dosage form 4 and comparative dosage form 5 were arranged for every 3 days. The scalp pruritus prevention effect was evaluated by the following evaluation criteria for one continuous use for two weeks, and the results are shown in Table 26.

[Evaluation Criteria]

Very good -5 points

Excellent -4 points

General -3 points

Failed -2 points

Very unqualified -1 point

As is apparent from the results of the above Table 26, the dosage form 4 containing ginsenoside Mc exhibited an excellent effect in preventing scalp itching.

[Test Example 21] Evaluation of the effect of potassium ion channel activity increase

Minoxidil, a hair loss treatment agent, is considered to be a potential mitochondrial potassium channel opener (K _ATP channel opener), which is a representative drug for androgenetic alopecia. In order to evaluate the mechanism of this minoxidil, the following test method was used in which tolbutamide (SIGMA A1DRICH, T0891) for blocking the mitochondrial potassium ion channel was treated in the fibroblasts constituting the dermis of the scalp. Inhibits cell proliferation, reopens potassium channels and restores cell proliferation.

In order to evaluate the function of the present composition as a mitochondrial potassium ion channel opener, a fibroblast cell line NIH3T3 (Mouse embryonic fibroblast cell line) cell line was used in the present invention. This cell line is a natural immortalized cell line isolated from a fibroblast cell line of NIH Swiss mouse embryo under the 3T3 protocol. The above cell strain was cultured in a Dulberaceae modified Eagle's medium (Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS for 24 hours in an incubator maintained at 5% carbon dioxide at 37 °C. NIH3T3 was placed in a 96-well plate, and cultured in a 37 ° C incubator for 24 hours, and then treated with 2.5 mM tolbutamide. After 10 minutes, 10 μM of minoxidil and ginsenoside Mc as a positive control group were respectively 2.5 ppm. At a concentration of 5 ppm and 10 ppm, the cell proliferation ability was measured 48 hours after the drug treatment using the WST-1 kit (Roche). The results are shown in Table 27 below.

As shown in the results of the above Table 27, when the ginsenoside Mc is treated, the proliferation of the fibroblasts is restored, and as the concentration of the ginsenoside Mc to be treated is increased, the cell proliferation ability is also increased. If the ginsenoside Mc is treated at 10 ppm, Then the extent of cell proliferation recovery is reached to the extent that it is treated with minoxidil.

[Test Example 22] Test of melanin production promoting effect of ginsenoside Mc

Add 5% fetal bovine serum, 100 IU of penicillin G, and 0.2 μM of TPA to RPMI medium, and place melanocytes (melan-a) in 24-well plates (24-well). The microtiter plate was dispersed at 50,000 cells/well. On the next day, ginsenoside Mc as a test substance was treated to the dispersed cells at a final concentration of 10 ppm or 50 ppm, 0.1% DMSO was treated as a negative control group, 100 μM IBMX was treated as a positive control group, and cultured at 37 ° C for 3 days. . After the completion of the culture, the wells were washed with phosphate buffered saline (PBS), and 1 N sodium hydroxide (NaOH) was added in an amount of 100 μl each time to dissolve the melanin in the cells. The absorbance of the dissolved melanin was measured by a plate reader using a microplate reader at 405 nm (Synergy 2, BioTek (VT, USA)). The results of the melanin production-promoting effect of ginsenoside Mc compared with the control group are shown in Table 28 below.

As is apparent from the results of the above Table 28, the ginsenoside Mc promotes melanin synthesis of melanocytes and increases the amount of melanin production, thereby exhibiting an excellent melanin production-promoting effect.

[Test Example 23] Effect of small eye deformity-related transcription factor (MITF) and tyrosinase expression in melanocytes of ginsenoside Mc

The cell strain of 501 mel was dispersed to a 6-well microtiter plate at 500,000 cells/well, and for each well, 0.1% of DMSO was treated as a negative control group, and IBMX 100 μM was treated as a positive control group, and as The test group treated ginsenoside Mc at 10 ppm, and cultured at 37 ° C for 24 hours, 48 hours, and 72 hours. For the protein so obtained, by Immunoblotting was performed with a small eye deformity-related transcription factor (MITF) and a tyrosinase antibody. Protein extraction and immunoblotting typically use standard methods employed by those skilled in the art. After the immunoblotting was completed, the results were compared with 100 of the negative control group, and the results are shown in Table 29 below.

As is apparent from the results of the above Table 29, ginsenoside Mc can enhance the expression of the small eye deformity-related transcription factor (MITF) and tyrosinase protein in melanocytes.

[Test Example 24] Evaluation of antibacterial activity of ginsenoside Mc

In order to evaluate the antibacterial activity of ginsenoside Mc, an antibacterial experiment was carried out. The specific method is as follows.

The strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were cultured in tryptic Soy Broth, Candida albicans. Aspergillus niger strain was cultured in Sabouraud Dextrose Broth. A dilution of 1/100 of the culture solution (1/10 of Candida albicans strain) to each medium was used as a test bacterial solution. Aspergillus niger will be used as a test bacterial solution with a spore suspension prepared at 2 x 108 cfu/ml.

0.15 ml of the above test bacterial solution was added to each 15 ml of the medium, uniformly mixed, and used as a diluted solution.

To a 16-well plate (96 well plate), a ginsenoside Mc10 ppm was placed in 16 μl each, and each was placed in a diluted solution of 184 μl. To each of the remaining wells, 100 μl of the diluted solution was placed. After uniformly mixing the mixture of No. 1 line, 100 μl was taken and placed in line No. 2, and after uniformly mixing, 100 μl was again extracted and placed in row No. 3, and 2-fold dilution was carried out in this manner.

Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were cultured in a thermostat at 32 ° C, Candida albicans and Aspergillus niger at 25 The culture was carried out in a thermostat of °C.

After 48 hours, the proliferation inhibition and suspension were confirmed by microscopy, and the minimum inhibitory concentration (MIC) value was determined and the results are shown in Table 30 below.

As is apparent from the results of the above Table 30, ginsenoside Mc exhibits an antibacterial activity against various strains, whereby it is predicted that ginsenoside Mc can function as a natural preservative or an antibacterial agent in the composition.

Claims (22)

A skin external preparation composition comprising: ginsenoside Mc as an active ingredient. The skin external preparation composition according to the first aspect of the invention, wherein the ginsenoside Mc is represented by the following Chemical Formula 1: The skin external preparation composition according to claim 1 or 2, wherein the ginsenoside Mc is contained in an amount of 0.001% by weight to 50% by weight based on the total weight of the composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is an anti-aging composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a skin elasticity increasing composition. The skin external preparation composition according to claim 1 or 2, wherein the composition is a skin wrinkle improving composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a moisturizing composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a skin barrier function-enhancing composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a skin keratinocyte differentiation-inducing composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a composition for improving acne. The skin external preparation composition according to the above aspect of the invention, wherein the composition is an antibacterial composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is an anti-inflammatory composition. The skin external preparation composition according to claim 1 or 2, wherein the composition is a composition for atopic skin improvement. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a gas color and skin color improving composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a composition for pore shrinkage. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a composition for regulating a sebum. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a skin problem improving composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a whitening composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a hair growth promoting composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a composition for preventing white hair. The skin external preparation composition according to the above aspect of the invention, wherein the composition is an antidandruff composition. The skin external preparation composition according to the above aspect of the invention, wherein the composition is a composition for a natural preservative.