TWI644691B - Blood plasma and blood cell separation device and separating method thereof - Google Patents

Abstract

A plasma blood cell separation device includes a first body, a plasma separation membrane, and a second body. The first body has an injection port. The plasma separation membrane is disposed in the first body. The second body is movably assembled to the first body and has a collection stage. The collection stage has a collection surface and a sampling slot. When a blood enters the plasma separation membrane from the injection port of the first body, a plasma in the plasma separation membrane is detached by the capillary force between the collection stage of the second body and the plasma separation membrane, and is separated by the first body A relative movement with the second body causes plasma to enter the sampling tank.

Description

Plasma blood cell separation device and separation method thereof

The present invention relates to a separation device and a separation method, and more particularly to a plasma blood cell separation device and a method of separating the same.

For the use of plasma for immunoassay, it is generally necessary to collect venous blood and then use a plasma centrifuge to perform plasma blood cell separation. Because plasma centrifuges are expensive and space-consuming, they are not suitable for use in pharmacy systems or home care testing. In order to facilitate the separation of plasma blood cells, there is currently a product for separating plasma using a plasma separation membrane, which utilizes the pore design of a plasma separation membrane to filter out blood plasma. In such a product for separating plasma using a plasma separation membrane, since the filtered plasma is still stored in the plasma separation membrane, it is generally necessary to use an additional absorbent material to suck out the plasma in the plasma separation membrane, and then The plasma is collected by squeezing the absorbent material. However, the above method requires a longer operation time because more steps are required. Again, because of plasma In addition to the separation of the membrane by the plasma, it is also required to be absorbed by the absorbent material, and the plasma cannot be completely extruded during the extrusion of the absorbent material, so that the plasma separated from the blood by the above-described method has a low utilization rate. In addition, because of the need to use an absorbent material, the consumables cost required for the above method is also high.

The present invention provides a plasma blood cell separation device which can efficiently and quickly obtain a desired plasma sample.

The invention also provides a plasma blood cell separation method, which allows a user to obtain a desired plasma sample in a short time with only a small amount of whole blood.

The plasma blood cell separation device of the present invention comprises a first body, a plasma separation membrane and a second body. The first body has an injection port. The plasma separation membrane is disposed in the first body. The second body is movably assembled to the first body and has a collection stage. The collection stage has a collection surface and a sampling slot. When a blood enters the plasma separation membrane from the injection port of the first body, a plasma in the plasma separation membrane is detached by the capillary force between the collection stage of the second body and the plasma separation membrane, and is separated by the first body A relative movement with the second body causes plasma to enter the sampling tank.

In an embodiment of the invention, the first body has a first surface facing the second body, wherein the first surface is convexly disposed on the second body. The injection inlet is placed on the film placement table, and the plasma separation membrane is fixed on the film placement table.

In an embodiment of the invention, the above-mentioned plasma separation membrane and collection When the stage is in a first relative position, the plasma separation membrane has a first overlapping surface with the collecting surface. When the plasma separation membrane and the collection stage are in a second relative position, the plasma separation membrane and the collection surface have a second overlapping surface, and the area of the second overlapping surface is smaller than the area of the first overlapping surface.

In an embodiment of the present invention, the second body has a second surface facing the first body, wherein the collecting stage protrudes from the first body to the second surface, and the sampling groove is recessed on the collecting surface.

In an embodiment of the invention, the first body has a first connecting portion, and the second body has a second connecting portion that is movably and separately connected to the first connecting portion.

The plasma blood cell separation method of the present invention is applied to a plasma blood cell separation device. The plasma blood cell separation device includes a first body, a plasma separation membrane, and a second body. The first body has an injection port, the plasma separation membrane is disposed in the first body, and the second body is movably assembled on the first body. The second body has a collection stage, and the collection stage has a collection surface and a sampling slot. A plasma blood cell separation method comprising the following steps. A blood is injected into the injection port of the first body to allow the plasma separation membrane to absorb blood from the injection port. The plasma in the separation membrane is separated from the plasma by the capillary force between the collection stage of the second body and the plasma separation membrane. A relative movement between the first body and the second body causes plasma to enter the sampling slot.

In an embodiment of the invention, the first body has a first surface facing the second body, wherein the first surface is convexly disposed on the second body. Note The inlet is placed on the film placement table, and the plasma separation membrane is fixed on the film placement table.

In an embodiment of the invention, when the plasma separation membrane and the collection stage are in a first relative position, the plasma separation membrane and the collection surface have a first overlapping surface. When the plasma separation membrane and the collection stage are in a second relative position, the plasma separation membrane and the collection surface have a second overlapping surface, and the area of the second overlapping surface is smaller than the area of the first overlapping surface.

In an embodiment of the present invention, the second body has a second surface facing the first body, wherein the collecting stage protrudes from the first body to the second surface, and the sampling groove is recessed on the collecting surface.

In an embodiment of the invention, the first body has a first connecting portion, and the second body has a second connecting portion that is movably and separately connected to the first connecting portion.

Based on the above, in the design of the plasma blood cell separation device of the present invention, the plasma in the plasma separation membrane is detached by the capillary force between the collection stage of the second body and the plasma separation membrane, and by the first body and The relative movement between the second bodies causes plasma to enter the sampling tank. In this way, the user can obtain the desired plasma sample by simply disassembling the second body of the first body. In other words, the plasma blood cell separation device of the present invention can efficiently and quickly obtain a desired plasma sample, and the plasma blood cell separation method using the plasma blood cell separation device of the present invention allows the user to use only a small amount of whole blood, that is, The desired plasma sample can be obtained in a short time.

The above described features and advantages of the invention will be apparent from the following description.

10‧‧‧ blood

20‧‧‧ plasma

30‧‧‧ blood collection needle

100‧‧‧ Plasma blood cell separation device

110‧‧‧First Ontology

112‧‧‧Injection

114‧‧‧First connection/slot

1142‧‧‧disconnected

116‧‧‧ first side

118‧‧‧film placement table

120‧‧‧ plasma separation membrane

130‧‧‧Second ontology

131‧‧‧ second side

132‧‧‧Collection of loading platforms

133‧‧‧Collection

134‧‧‧Sampling tank

135‧‧‧Second connection/bump

A1‧‧‧ first overlapping surface

A2‧‧‧ second overlapping surface

P1‧‧‧ first relative position

P2‧‧‧ second relative position

FIG. 1A is a perspective exploded view of a plasma blood cell separation device according to an embodiment of the invention.

FIG. 1B is a perspective exploded view showing the plasma blood cell separation device of FIG. 1A from another perspective.

FIG. 1C is a top plan view showing the plasma separation membrane of FIG. 1A in a first relative position with the collection stage.

FIG. 1D is a top plan view showing the plasma separation membrane of FIG. 1A in a second relative position with the collection stage.

2A-2D are schematic front views of a plasma blood cell separation method according to an embodiment of the present invention.

FIG. 3A is a schematic diagram showing the distribution of plasma in a first relative position of the plasma separation membrane and the collection stage.

FIG. 3B is a schematic diagram showing the distribution of plasma in a second relative position of the plasma separation membrane and the collection stage.

3C is a top plan view of the second body of the plasma blood cell separation device of FIG. 2D.

1A is a diagram showing a blood cell separation device according to an embodiment of the present invention. A three-dimensional exploded view of the setting 100. FIG. 1B is a perspective exploded view of the plasma cell separation device 100 of FIG. 1A from another perspective. Referring to FIG. 1A and FIG. 1B simultaneously, in the embodiment, the plasma blood cell separation device 100 includes a first body 110, a plasma separation membrane 120, and a second body 130. The first body 110 is movably assembled to the second body 130 and has an injection port 112. The second body 130 has a collecting stage 132. The collecting stage 132 has a collecting surface 133 and a sampling slot 134. The plasma separation membrane 120 is disposed on the first body 110 and is movable along with the first body 110.

More specifically, the first body 110 of the present embodiment has a first surface 116 facing the second body 130, wherein the first surface 116 protrudes from the second body 130 with a film placement table 118, and the injection port 112 is disposed at The film is placed on the stage 118. The second body 130 has a second surface 131 facing the first body 110 , wherein the collecting stage 132 protrudes from the first body 110 on the second surface 131 . The sampling slot 134 is recessed on the collecting surface 133. The plasma separation membrane 120 is fixed to the film placement table 118 of the first body 110 and faces the collection surface 133 of the collection stage 132. When the plasma separation membrane 120 faces the collection surface 133, a capillary phenomenon can be generated between the plasma separation membrane 120 and the collection surface 133.

In this embodiment, the first body 110 has a first connecting portion 114, and the second body 130 has a second connecting portion 135 that is movably and separately connected to the first connecting portion 114. In an embodiment, as shown in FIG. 1A and FIG. 1B , the first connecting portion 114 can be a sliding slot 114 , and the sliding slot 114 has a disengagement opening 1142 . The second connecting portion 135 can be a protrusion 135. The protruding block 135 can move in the sliding slot 114 and can follow the sliding slot. 114 moves to the escape port 1142 to be separated from the chute 114.

In this embodiment, the plasma separation membrane 120 is fixed on the first body 110, and the first body 110 can move the plasma separation membrane 120 relative to the second body 130 to have a plasma separation membrane 120 and the collection stage 132. The first relative position P1 and the second relative position P2.

FIG. 1C is a top plan view showing the plasma separation membrane 120 of FIG. 1A and the collection stage 132 at a first relative position P1. FIG. 1D is a top plan view showing the plasma separation membrane 120 of FIG. 1A and the collection stage 132 at a second relative position P2. In this embodiment, as shown in FIG. 1C, when the plasma separation membrane 120 and the collection stage 132 are located at the first relative position P1, the plasma separation membrane 120 and the collection surface 133 have a first overlapping surface A1; 1D, when the plasma separation membrane 120 and the collection stage 132 are located at the second relative position P2, the plasma separation membrane 120 and the collection surface 133 have a second overlapping surface A2, wherein the area of the second overlapping surface A2 is smaller than the first The area of an overlapping surface A1.

2A-2D are schematic front views of a plasma blood cell separation method according to an embodiment of the present invention. 3A and 3B are schematic diagrams showing plasma distributions of the plasma separation membrane 120 and the collection stage 132 at a first relative position P1 and a second relative position P2, respectively. 3C is a top plan view of the second body 130 of the plasma cell separation device 100 of FIG. 2D. Referring to FIG. 2A and FIG. 2B simultaneously, when a blood 10 is injected from the injection port 112 of the first body 110, the blood 10 flows from the injection port 112 of the first body 110 to the plasma disposed on the first body 110. Film 120. Here, the blood 10 is, for example, whole blood, which means that it contains plasma, blood cells and platelets. The blood 10 therein is, for example, 50 microliters (ul).

Next, referring to FIG. 2B and FIG. 3A simultaneously, the inlet side of the plasma separation membrane 120 (ie, the side near the injection port 112) has a pore diameter of about 100 μm, and the outlet side of the plasma separation membrane 120 (ie, near the collection stage 132). The side of the side has a pore size of about 1 micrometer, so when the blood 10 passes through the plasma separation membrane 120, red blood cells, platelets, and the like are blocked by the plasma separation membrane 120 and cannot pass through the plasma separation membrane 120. In this way, only plasma 20 can pass through the plasma separation membrane 120 to achieve the purpose of separating plasma 20.

In the present embodiment, when the blood 10 is injected from the injection port 112 of the first body 110, the plasma separation membrane 120 and the collection stage 132 are located at the first relative position P1, that is, the plasma separation membrane 120 faces the collection surface 133. And there is a first overlapping surface A1 therebetween, as shown in FIG. 1C. The collection surface 133 of the collection stage 132 and the plasma separation membrane 120 generate a capillary force between the first overlapping surface A1, and the plasma 20 in the plasma separation membrane 120 is attracted to the plasma separation membrane 120. At this time, as shown in FIG. 3A, the plasma 20 detached from the plasma separation membrane 120 is distributed on the first overlapping surface A1 of the collecting surface 133 of the collecting stage 132.

After that, referring to FIG. 2C and FIG. 3B, a relative movement between the first body 110 and the second body 130, such as relative rotation, causes the plasma 20 distributed on the first overlapping surface A1 of the collecting surface 133 to enter the collection. Inside the sampling slot 134 on the stage 132. Specifically, when the first body 110 is rotated relative to the second body 130, the area of the overlapping surface between the collecting surface 133 of the collecting stage 132 and the plasma separation membrane 120 becomes small, so that the plasma 20 can be distributed on the collecting surface 133. The area on the surface is relatively small, It is not possible to accommodate all of the plasma 20 detached from the plasma separation membrane 120, so that the excess plasma 20 enters the sampling tank 134 and is stored in the sampling tank 134. In other words, in this embodiment, the plasma 20 is stored in the storage tank 134 of the second body 130 through the relative movement between the first body 110 and the second body 130. Of course, in other embodiments not shown, the relative movement between the first body and the second body may also be relatively movable, which is still within the scope of the present invention.

Referring to FIG. 1D and FIG. 3B, when the first body 110 drives the plasma separation membrane 120 to move relative to the second body 130, and the plasma separation membrane 120 and the collection stage 132 are located at the second relative position P2, the plasma separation membrane 120 is collected. The surface 133 has a second overlapping surface A2. Since the second overlapping surface A2 is smaller than the first overlapping surface A1, the plasma 20 originally distributed on the first overlapping surface A1 cannot be completely distributed on the second overlapping surface A2, so the excess plasma 20 Will enter the sampling slot 134.

Finally, please refer to FIG. 2D and FIG. 3C simultaneously, disassembling the first body 110 and the second body 130 to separate the first body 110 from the second body 130. At this time, the desired plasma 20 can be collected in the sampling tank 134 of the second body 130 through the blood collection needle 30 or a pipette (not shown).

In short, in the design of the plasma cell separation apparatus 100 of the present embodiment, the first body 110 has the function of injecting the injection port 112 of the blood 10 and fixing the plasma separation membrane 120, and the plasma separation membrane 120 has the plasma 20 The function of separation in the blood 10, and the second body 130 has a function of taking out the plasma 20 from the plasma separation membrane 120 and collecting the plasma 20. When the blood 10 enters the plasma separation membrane 120 from the injection port 112 of the first body 110, the collection by the second body 130 The capillary force between the stage 132 and the plasma separation membrane 120 causes the plasma 20 in the plasma separation membrane 120 to be detached, and the plasma 20 enters the sampling tank 134 by the relative movement between the first body 110 and the second body 130. . In this way, after injecting the blood 10, the user only needs to rotate and disassemble the first body 110 and the second body 130 to obtain the desired plasma 20 sample.

In other words, the plasma blood cell separation device 100 of the present embodiment does not need to use a conventional absorbent material to absorb plasma, so that in addition to reducing the cost of consumables and reducing plasma residue to improve separation utilization, the operation time can also be reduced. The desired plasma 20 sample is obtained efficiently and quickly. Further, by using the plasma blood cell separation method of the plasma blood cell separation apparatus 100 of the present embodiment, the user can obtain the desired plasma 20 sample in a short time with only a small amount of whole blood.

In summary, in the design of the plasma blood cell separation device of the present invention, the plasma in the plasma separation membrane is detached by the capillary force between the collection stage of the second body and the plasma separation membrane, and by the first body The relative movement with the second body causes plasma to enter the sampling tank. In this way, after injecting blood, the user only needs to rotate and disassemble the second body of the first body to obtain the desired plasma sample. In other words, the plasma blood cell separation device of the present invention can efficiently and quickly obtain a desired plasma sample, and the plasma blood cell separation method using the plasma blood cell separation device of the present invention allows the user to use only a small amount of whole blood, that is, The desired plasma sample can be obtained in a short time.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the invention, and any one of ordinary skill in the art without departing from the invention. In the spirit and scope, the scope of protection of the present invention is subject to the definition of the appended patent application.

Claims (10)

A plasma blood cell separation device comprising: a first body having an injection port; a plasma separation membrane disposed on the first body; and a second body movably assembled on the first body and having a collection stage having a collection surface and a sampling tank, wherein when a blood enters the plasma separation membrane from the injection port of the first body, the collection stage of the second body and the The plasma separation membrane detaches a plasma in the plasma separation membrane by capillary force, and the plasma enters the sampling tank by a relative movement between the first body and the second body. The plasma blood cell separation device of claim 1, wherein the first body has a first surface facing the second body, and the first surface has a film placement table protruding toward the second body. The injection port is disposed on the film placement table, and the plasma separation film is fixed on the film placement table. The plasma blood cell separation device according to claim 1, wherein when the plasma separation membrane and the collection stage are in a first relative position, the plasma separation membrane and the collection surface have a first overlapping surface. And when the plasma separation membrane and the collection stage are in a second relative position, the plasma separation membrane and the collection surface have a second overlapping surface, and the area of the second overlapping surface is smaller than the first overlap. The area of the face. The plasma blood cell separation device of claim 1, wherein the second body has a second surface facing the first body, the collection stage protruding from the first body on the second surface The sampling slot is recessed on the collecting surface. The plasma blood cell separation device of claim 1, wherein the first body has a first connecting portion, and the second body has a second connecting portion movable and separately from the first connecting portion connection. A plasma blood cell separation method is applied to a plasma blood cell separation device, the plasma blood cell separation device comprising a first body, a plasma separation membrane and a second body, the first body having an injection port, the plasma separation membrane configuration On the first body, the second body is movably assembled on the first body, and the second body has a collecting stage having a collecting surface and a sampling tank, the plasma The blood cell separation method includes: injecting a blood into the injection port of the first body, so that the plasma separation film absorbs the blood from the injection port, and the collection stage and the plasma separation film of the second body The capillary force between the two separates from the plasma in the plasma separation membrane; and a relative movement between the first body and the second body causes the plasma to enter the sampling tank. The plasma blood cell separation method of claim 6, wherein the first body has a first surface facing the second body, and the first surface is convexly provided with a film placing table toward the second body. The injection port is disposed on the film placement table, and the plasma separation film is fixed on the film placement table. The plasma blood cell separation method according to claim 6, wherein when the plasma separation membrane and the collection stage are in a first relative position, the plasma separation membrane and the collection surface have a first overlapping surface. And when the plasma separation membrane and the collection stage are in a second relative position, the plasma separation membrane and the collection surface have a second overlapping surface, and the area of the second overlapping surface is smaller than the first overlap. The area of the face. The plasma blood cell separation method of claim 6, wherein the second body has a second surface facing the first body, the collection stage protruding from the first body on the second surface The sampling slot is recessed on the collecting surface. The plasma blood cell separation method of claim 6, wherein the first body has a first connecting portion, and the second body has a second connecting portion movable and separately from the first connecting portion connection.