TWI625320B - Method for producing an amino acid containning product - Google Patents

Abstract

A method for producing an amino acid-containing product, comprising: a feeding step, a hydrolysis step, and a separating step, wherein the feeding step is to place the biological fragments into a gas-tight tank, and the hydrolysis step is performed in the gas-tight tank The biomass fragment is agitated in an atmosphere having a temperature of 145 ° C to 180 ° C and a saturated water vapor pressure of 10 to 22 atm and a time of 40 to 120 minutes to hydrolyze the biological fragments to obtain an amine-containing compound. The base acid product, and a separation step, separates the amino acid-containing product to provide an oily product, a liquid product containing an amino acid, and a solid product containing an amino acid.

Description

Method for producing amino acid-containing product

This invention relates to a process for the manufacture of an amino acid-containing product, and more particularly to a process for the production of an amino acid-containing product from a hydrolyzed organism.

The amino acid is an organic compound containing an amine group and a carboxyl group, and is a basic unit constituting a protein. In recent years, amino acids have been widely used in agriculture, animal husbandry, and medical fields. Especially in agriculture, they can enhance the antibodies of crops, and are the most important source of nutrients for crop growth. For animal feeding, crops, and fertilizers. Quite good utility.

A general method for producing an amino acid includes a hydrolysis method which degrades a protein of a living body into an amino acid, and the product obtained by the production method contains about 20 g of an amino acid per 100 g. The hydrolysis method is further divided into an acid or alkali hydrolysis method, a microorganism hydrolysis method, and an enzymatic hydrolysis method. The acid or alkali hydrolysis method is mostly carried out by adding an acid solution or an alkali solution to hydrolyze the living body to obtain an amino acid. Although this method of production can quickly obtain an amino acid, some of the amino acids are subjected to acid or alkali destruction, resulting in a halving of the nutritional value, resulting in poor quality of the amino acid. Furthermore, the acid or lye used in this manufacturing method has the disadvantage of polluting the environment and endangering the human body.

The microbial hydrolysis method is carried out by using a specific microorganism to culture the organism, and secreting the enzyme to decompose the organism to produce an amino acid. Although this method does not destroy the amino acid and does not cause environmental pollution, the reaction time is very long, and even takes several months, which is not economical, and an unpleasant odor is generated during the reaction.

Therefore, enzymatic hydrolysis has become the current trend in the manufacture of amino acids. The enzymatic hydrolysis method utilizes an active enzyme to catalyze a hydrolysis reaction, the reaction conditions are mild, and the amino acid is not destroyed. However, although the enzymatic hydrolysis method shortens the reaction time of the microbial hydrolysis method, it takes tens of hours of lengthy reaction time. Moreover, in the reaction process of the enzymatic hydrolysis method, the activity of the enzyme is very important, so there are certain strict requirements on the ambient temperature and the pH value. In addition, the enzyme is selective for proteins, and a specific enzyme must be selected for the type of protein before use. Therefore, the enzymatic hydrolysis method has the disadvantages of severe reaction conditions and difficulty in maintaining the activity of the enzyme.

Accordingly, it is an object of the present invention to provide a method for producing an amino acid-containing product which can reduce environmental pollution, reduce damage to amino acids, and at the same time, can have high reaction speed and simple steps.

The technical means for solving the problems of the prior art provides a method for producing an amino acid-containing product, comprising: a feeding step of placing the biological fragments into a gas-tight tank; a hydrolysis step, In the airtight tank, the biological debris is agitated at an atmosphere temperature of 145 ° C to 180 ° C and a saturated water vapor pressure of 10 to 22 atmospheres and a time of 40 to 120 minutes to make the biological debris Performing a hydrolysis reaction to obtain an amino acid-containing product; and a separation step of separating the amino acid-containing product to obtain an oily product, a liquid product containing an amino acid, and an amino acid-containing acid a solid product in which, per 100 g of the liquid product obtained by the separation step contains 25 g to 35 g of an amino acid, and in the method of the method for producing an amino acid-containing product, there is no additional Add substances that affect the reaction rate.

In one embodiment of the invention, a method of making an amino acid-containing product is provided, wherein the temperature is from 160 ° C to 180 ° C.

In one embodiment of the invention, a method of making an amino acid-containing product is provided, wherein the saturated water vapor pressure system is from 20 to 22 atmospheres.

In an embodiment of the invention, there is provided a method for producing an amino acid-containing product, further comprising a pressure relief pumping step between the hydrolysis step and the separation step, wherein the gas-tight tank is depressurized to Atmospheric pressure, and vacuum extraction of the airtight tank.

In one embodiment of the present invention, there is provided a method for producing an amino acid-containing product, wherein in the separating step, the amino acid-containing acid product is placed for a predetermined time by means of standing precipitation to cause the inclusion The amino acid product is separated into an oily product, a liquid product, and a solid product by specific gravity.

In one embodiment of the invention, a method of making an amino acid-containing product is provided, wherein the time is from 65 to 85 minutes.

In one embodiment of the invention, a method of making an amino acid-containing product is provided, the material affecting the rate of reaction being a catalyst.

In one embodiment of the present invention, a method for producing an amino acid-containing product is provided, in which the crushed animal carcass is placed as the biological debris into the airtight tank.

In one embodiment of the invention, a method of making an amino acid-containing product is provided, the body fragment being a body of a broken animal.

In one embodiment of the invention, a method of making an amino acid-containing product is provided which is used directly in a fertilizer for fertilization.

The method for producing an amino acid-containing product of the present invention is simple to operate, and shortens the hydrolysis reaction time of the organism by high temperature, high pressure, and environmental catalytic reaction filled with saturated water vapor pressure, and obtains a higher content of amino acid. . In addition, no additional substances affecting the reaction rate are added during the entire reaction process, which not only avoids the destruction of the environment and the nature of the amino acid by acid and alkali substances and other chemicals, but also can be safely applied to organisms or other aspects. For example, the liquid product can be used directly as a fertilizer for crops, while the solid product can be processed for fertilization and the oily product treated to produce biodiesel for reuse.

In addition, the method for producing an amino acid-containing product of the present invention uses animal carcasses as a raw material, thereby avoiding environmental problems caused by the treatment of animal carcasses by burial or incineration.

S10‧‧‧ Feeding steps

S20‧‧‧ hydrolysis step

S21‧‧‧ Pressure relief pumping step

S30‧‧‧Separation step

1 is a flow chart showing a method of producing an amino acid-containing product according to an embodiment of the present invention.

Fig. 2 is a flow chart showing a method of producing an amino acid-containing product according to another embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described based on Figs. 1 to 2 . This description is not intended to limit the embodiments of the invention, but is an embodiment of the invention.

As shown in FIG. 1, a method for producing an amino acid-containing product according to an embodiment of the present invention is a method for producing an amino acid-containing product by hydrolysis of a biological fragment, comprising a feeding step S10. a hydrolysis step S20 and a separation step S30.

First, the biological debris that has been crushed by the crushing device is placed in the airtight tank through the inlet of the airtight tank by the conveying device (feeding step S10).

In detail, the airtight tank system is a gastight boiler and has a plurality of stirring rods. The organism fragments are frozen pig carcasses. The amino acid content of animal proteins is higher than that of vegetable proteins, and a higher content of amino acids can be produced. Of course, the invention is not limited thereto. The biological debris can also be a plant, fish or other animal carcass, and can be a specific part of the organism, such as feathers or fish scales, to produce a specific kind of amino acid. In addition, the biological debris can also be selected according to the source of the organism.

Then, the inlet port is closed, and the environment in the airtight tank is controlled to be in an environment of a temperature of 145 ° C to 180 ° C and a saturated water vapor pressure of 10 to 22 atmospheres, and the time is 40 to 120 minutes. The agitating rod stirs the fragment of the organism to cause the fragment of the organism to accelerate and uniformly undergo a hydrolysis reaction to obtain an amino acid-containing product (hydrolysis step S20).

In detail, in this high temperature and high pressure environment, macromolecules in the body fragments, including fat, starch and protein, will become loose after a period of time, plus saturated water vapor at a high density. The vapor molecules move thermally, and the rapid impact of the macromolecules that have loosened the structure, causing them to be gradually broken into small molecular weight substances, such as protein hydrolysis to amino acids and their derivatives, fat hydrolysis to glycerol and The fatty acid effectively reduces the overall reaction time to obtain the amino acid-containing product. Preferably, the temperature can be set to 160 ° C to 180 ° C, and the saturated water vapor pressure can be set to 20 to 22 atmospheres, and the time can be 65 to 85 minutes.

Finally, the amino acid-containing product is separated to obtain an oily product, a liquid product containing an amino acid, and a solid product containing an amino acid (separation step S30).

In detail, in the present embodiment, the amino acid-containing product is allowed to stand for a predetermined period of time by standing precipitation, so that the amino acid-containing product is separated into oily products, liquid products, and solids by specific gravity. The product was then taken out and layered. Of course, the invention is not limited thereto, and other separation methods such as centrifugation may also be used.

By performing the above steps, it is possible to obtain 25 to 35 g of amino acid per 100 g of the liquid product.

It is worth mentioning that in the method for producing an amino acid-containing product of the present invention, no additional substances affecting the reaction rate, such as a catalyst, are added, thereby achieving cost saving, no environmental pollution, and disposal of harmful substances. efficacy.

In this embodiment, the hydrolysis step (step S20) is performed by stirring the living body debris in the airtight tank at an atmosphere having a temperature of 180 ° C and a saturated water vapor pressure of 10 to 22 atmospheres. The hydrolysis reaction can be sufficiently carried out in a time of 80 minutes. Of course, the present invention is not limited thereto, and the hydrolysis step (step S20) can adjust the temperature, pressure, and time within a predetermined range depending on the species of the living body.

As shown in Fig. 2, a method for producing an amino acid-containing product according to another embodiment of the present invention is substantially the same as the previous embodiment except that the hydrolysis step (step S20) and separation are carried out. Between the steps (step S30), a pressure relief pumping step (step S21) is further included, the airtight tank is depressurized to a normal pressure, and the airtight tank is vacuum-extracted to extract the The water vapor within the gas-tight tank can reduce the water content of the amino acid-containing product prior to obtaining the amino acid-containing product.

As shown in Table 1 below, in an embodiment of another embodiment of the present invention, the liquid product obtained by the separation step is taken out to carry out identification analysis of the amino acid content.

(This analysis report is commissioned by the Central Livestock Association (NAIF) analysis, application number: 10307PP00197)

It can be seen from Table 1 that the liquid product has at least eighteen amino acids as listed, and that the liquid product contains 3,202,5.9 mg of amino acid per 100 g.

From the above results, it was confirmed that the liquid product obtained by the present invention contains 32 g or more of an amino acid. Therefore, the liquid product obtained by the method of the present invention has a relatively high content of amino acid, and can effectively improve the quality of the product.

Preferably, the liquid product is directly applied to the fertilized fertilizer, and is applied to the plant with a high content of amino acid and no harmful substances. In this embodiment, the liquid product is directly used for fertilizing the fertilizer, and the solid product also contains an amino acid which can be processed for fertilization, and the oily product can be processed to produce biodiesel. Utilize, thereby achieving the effect of no pollution in the manufacturing process and high utilization of the product.

By the above method, in the present invention, the environment of high temperature and high pressure and full of saturated vapor pressure is utilized to catalyze the hydrolysis reaction time of the living body to 40 to 120 minutes, and no harmful substance is added in the process, and a higher content is obtained ( The liquid product of the amino acid-containing product produced by other hydrolysis processes currently in the industry generally contains only about 20 grams of amino acid per 100 grams, and the liquid product obtained by the present invention contains about 25 to 100 grams per 100 grams. 35 grams of amino acid) and does not have the amino acid product of the pest, and takes into account the environmental benefits. Moreover, the present invention can hydrolyze animal carcasses at a high temperature to produce a usable substance, so as to avoid secondary pollution caused by the liquid or gas discharge of the liquid in the past or the incineration method to maintain a good quality of life for human beings.

The above description and description are only illustrative of the preferred embodiments of the present invention, and those of ordinary skill in the art can make other modifications in accordance with the scope of the invention as defined below and the description above, but such modifications should still be It is within the scope of the invention to the spirit of the invention.

Claims (9)

A method for producing an amino acid-containing product, comprising: a feeding step of placing a body debris into a gas-tight tank; and a hydrolysis step in the gas-tight tank at a temperature of 145 ° C to Stirring the organism at 180 ° C and a saturated water vapor pressure of 10 to 22 atmospheres and for 40 to 120 minutes to hydrolyze the organism fragments to obtain an amino acid-containing product; and a separation step Separating the amino acid-containing product to obtain an oily product, a liquid product containing an amino acid, and a solid product containing an amino acid, wherein each 100 g of the separation step is obtained. The liquid product contains 25 to 35 grams of amino acid, and in the method of the method for producing an amino acid-containing product, there is no additional addition of a substance affecting the reaction rate, and in the hydrolysis step and the separation step Between the two, a pressure relief pumping step is performed, the airtight tank is released to a normal pressure, and the airtight tank is vacuum-extracted. The method for producing an amino acid-containing product according to claim 1, wherein in the hydrolysis step, the temperature is from 160 ° C to 180 ° C. The method for producing an amino acid-containing product according to claim 1, wherein in the hydrolysis step, the saturated water vapor pressure is from 20 to 22 atm. The method for producing an amino acid-containing product according to claim 1, wherein in the separating step, the amino acid-containing acid product is allowed to stand for a predetermined time by means of standing precipitation to cause the amino acid-containing acid. The product is separated into an oily product, a liquid product, and a solid product by specific gravity. The method for producing an amino acid-containing product according to claim 1, wherein in the hydrolysis step, the time is from 65 to 85 minutes. The method for producing an amino acid-containing product according to claim 1, wherein the substance affecting the reaction rate is a catalyst. The method for producing an amino acid-containing product according to claim 1, wherein in the feeding step, the crushed animal carcass is placed as the biological debris into the airtight tank. The method for producing an amino acid-containing product according to claim 1, wherein the biological fragment is a corpse of a broken animal. The method for producing an amino acid-containing product according to claim 1, wherein the liquid product is directly used for fertilizing the fertilizer.