TWI614062B - Hydrolyzate extraction method - Google Patents

Abstract

The invention discloses a method for extracting a hydrolyzate by combining an electromagnetic field and an enzyme, the method comprising: providing a biological raw material; providing an electromagnetic field to the biological raw material; and adding an enzyme to the biological raw material, and performing a hydrolysis step under the electromagnetic magnetic field to obtain hydrolysis Things.

Description

Method for extracting hydrolysate

The present invention relates to a method for extracting a hydrolyzate, and more particularly to a method for extracting a functional small molecule hydrolyzate by subjecting a biological material such as an animal or a plant to a specific electromagnetic field environment for enzymatic hydrolysis.

With the evolution of modern technology, extraction technology has been widely used in the biotechnology industry, such as the development of health foods, such as health foods that are commonly added on the market, such as grape seed extract, burdock root extract, or seaweed extract; or It is the application of cosmetic care products, such as collagen extracts for skin, acanthopanax extract, various plant essential oil extracts, and the like.

In the above industrial applications, active ingredients in animals, plants, algae, or microorganisms need to be extracted using appropriate and good extraction techniques. Conventional extraction methods use solvent extraction, which often takes a lot of time and uses a large amount of solvent to separate the effective components. However, the extraction efficiency and extraction purity are not ideal, and the use of solvents often causes environmental problems. Therefore, extraction techniques that are simple and have high extraction efficiency and extraction concentration are required for the biotechnology industry.

However, various kinds of biological cells have different characteristics. For example, different plant cells have different toughness in their cell walls, and thus the extraction methods of active ingredients in different plant cells are also different. There is currently no single extraction method available for all cells.

In view of the above problems of the prior art, it is an object of the present invention to provide a method for extracting a hydrolyzate, particularly a method for extracting a functional small molecule hydrolysate by combining an electromagnetic field and an enzyme. The method for extracting a hydrolyzate of the present invention comprises: providing a biological material; providing an electromagnetic field to the biological material; and adding the enzyme to the biological material, and performing a hydrolysis step under an electromagnetic field to obtain a hydrolyzate. The biological raw material comprises animals, plants and microorganisms; the electromagnetic field voltage is 500 to 10000V, and the current is between 3 mA and 50 mA; the electromagnetic field is a direct current electromagnetic field or a pulsed electromagnetic field, and the frequency of the pulsed electromagnetic field is 10 Hz-2000 Hz.

According to the above, wherein the biological material comprises animals, plants, microorganisms; and the enzyme may be selected from the group consisting of a sugar decomposition enzyme, a pectinase, a cellulase, a hemicellulase, a xylase, a mannosidase, a galactosidase, and a starch. Enzyme, disaccharidase, glucosease, glucanase, chitinase, microbial extract enzyme, acid protease, protamex, alkaline protease (alcalase), papain, flavourzyme One or more of the group consisting of trypsin, bromelain, pepsin, a combination of proteases and isoflavone-degrading enzymes.

Preferably, the method further comprises mixing the biological material with water in a predetermined ratio.

Preferably, the predetermined ratio of the biological material: water may be from 1:0.1 to 1:100.

Preferably, the method of extracting a hydrolyzate of the present invention may further comprise heating the biological material.

Preferably, in the method of extracting a hydrolyzate of the present invention, the voltage of the electromagnetic field may be 500 to 10000V.

Preferably, the electromagnetic field may comprise a positive high voltage direct current electromagnetic field, a negative high voltage direct current electromagnetic field, or a pulsed high voltage electromagnetic field.

Preferably, in the method of extracting hydrolysate, the hydrolysis step is carried out by hydrolysis of the enzyme at a temperature of from 1 to 80 ° C for from 1 second to 96 hours.

Preferably, the concentration of the enzyme may range from 0.001 to 20% w/v of the total reaction volume.

Preferably, an ultrasonic wave may be further provided, wherein the ultrasonic wave may be 20-100 KHz.

Preferably, the method provided by the present invention may further comprise providing an ultrahigh pressure, wherein the ultrahigh pressure is 100-600 MPa.

According to the method of extracting a hydrolyzate of the present invention, it may have one or more of the following advantages:

(1) The method for extracting a hydrolyzate of the present invention utilizes a method of hydrolyzing a biological material in a specific electromagnetic field environment in parallel with physical properties and chemical properties, whereby the concentration of the active material in the hydrolyzed extract can be increased.

(2) The method for extracting a hydrolyzate of the present invention is a small molecule activated extraction technique combining an electromagnetic field and an enzyme, which can be hydrolyzed by different specific electromagnetic fields with different enzymes for different biological materials, so that it can be completely hydrolyzed. It is an amino acid and a short-chain peptide to improve the flavor of the hydrolyzate, so it can be widely used for the extraction of different kinds of biological raw materials, and then applied to the development and manufacture of various health foods or cosmetic products.

(3) The method for extracting a hydrolyzate of the present invention, which mixes the biological material with water in a desired ratio, and is more environmentally friendly than the conventional method using an organic solvent.

(4) The method for extracting hydrolyzate of the present invention can effectively inhibit the growth of microorganisms under a specific electromagnetic field, ensure the occurrence of spoilage during the extraction of the enzyme, and improve the effective concentration and safety of the extraction.

In order to better understand the above and other aspects of the present invention, the preferred embodiments are described below, and in conjunction with the drawings, the detailed description is as follows:

100‧‧‧Electromagnetic field induction device

110‧‧‧Transformation controller

111‧‧‧ Waveform Modulator

120‧‧‧Storage equipment

121‧‧‧ extraction tank

122‧‧‧Insulation device

123‧‧‧heating temperature control device

124‧‧‧Insulation

130‧‧‧Power supply

S210-S250‧‧‧Steps

Fig. 1 is a view showing an electromagnetic field inducing device used in the present invention.

Fig. 2 is a flow chart showing a method of extracting a hydrolyzate according to an embodiment of the invention.

The invention will be further described in detail by the following preferred embodiments and the accompanying drawings. It should be noted that the experimental data disclosed in the following embodiments are for explaining the technical features of the present invention, and are not intended to limit the manner in which they can be implemented.

Please refer to FIG. 1, which is illustrated as an electric field induced device 100 for use in the present invention to generate a specific electromagnetic field. The electromagnetic field inducing device 100 includes a modulation controller 110, and the input AC voltage and current can be converted into positive/negative voltages, pulse voltages and currents of different output frequencies through the modulation controller 110, and waves can also be utilized. The type modulator 111 adjusts the electromagnetic wave pattern and transmits voltages and currents of different output frequencies to the storage sink 120 through the wires. The storage device 120 includes an internal extraction tank 121, a heat retention device 122, a heating temperature control device 123, and an insulation device 124. The active substance to be extracted (i.e., animal biological material or plant biological material) can be placed in the extraction tank 121. The heat retaining device 122 is used to isolate the temperature of the extraction tank and the outside during the extraction process to maintain a constant temperature inside the extraction tank. The heating control unit 123 applies a specific temperature to the inside of the extraction tank 121. The insulating device 124 is used to insulate the object in contact with the storage device 120 (e.g., when placed on the ground, from the ground).

During the extraction process, when the extraction tank 121 receives the specific modulation voltage Vm and the current Am, an electromagnetic field can be formed in the extraction tank 121. The electromagnetic field can be a direct current electromagnetic field or a pulsed electromagnetic field, and thus the active material to be extracted is in a specific electromagnetic field. environment of. In a particular embodiment of the invention, the modulation controller 110 can convert the power supply 130 to a modulated voltage Vm having a very low current and utilize the modulated voltage Vm to induce a particular desired electromagnetic field environment. For example, when the modulation voltage Vm is a DC voltage of +1000 V and a DC current of almost zero amperes, the electromagnetic field is a high voltage plus electric field, and when the modulation voltage Vm is -1000 V DC The electromagnetic field is a high voltage minus electric field when the voltage and a direct current of almost zero amperes. Or, when the modulation voltage Vm is a DC voltage of 1000V and a DC current of almost zero amperes, and the pulse period/frequency is adjusted from 0.5ms to 100ms/10Hz-2000Hz, the specific electromagnetic field environment is a pulsed high voltage electromagnetic field (high Voltage alternating electric field). Therefore, the electromagnetic field used in the present invention may be a positive high voltage direct current electromagnetic field, a negative high voltage direct current electromagnetic field, or a pulsed high voltage electromagnetic field, but the invention is not limited thereto.

Next, a method for generating an electromagnetic field by the above-described electromagnetic field inducing device for extracting a hydrolyzate by the above-described electromagnetic field inducing device will be described in detail, and will be described with reference to FIG.

The method of extracting a hydrolyzate of the present invention comprises, as shown in Fig. 2, a flow chart of a method of extracting a hydrolyzate according to an embodiment of the invention. In an embodiment of the present invention, a method of extracting a hydrolyzate comprises: providing a biological material; mixing the biological material with water in a predetermined ratio; providing an electromagnetic field to the biological material; and adding the enzyme to the biological material and performing hydrolysis under an electromagnetic field The step further obtains a hydrolyzate.

In view of the above, an embodiment is provided in accordance with an embodiment of the present invention, and reference is made to FIG. 2, which is a flow chart of a method of extracting a hydrolyzate according to an embodiment of the invention. The embodiment is as follows: the biological material is mixed with water in a predetermined ratio (S210), wherein the biological material may be an animal biological material, a plant biological material or a microbial biological material. Further, the animal biological material may include poultry, livestock, fish, Shellfish and insects; plant biomaterials may contain Chinese herbal medicines and fruits and vegetables; microbial biomass materials may include algae, bacteria, fungi and mites. The mixing may be homogenized by a homogenizer, or may be mixed by means of a fruit-and-grocer machine or by mashing the raw material. The preferred embodiment is homogenized by a homogenizer, but the invention is not limited thereto. An electromagnetic field is then provided to give the mixture of the biological material and water (S220), wherein the voltage of the electromagnetic field may be 500-10000V. Furthermore, the enzyme is added to the biological material (S230), wherein the enzyme may comprise, a carbohydrate decomposition enzyme, a pectinase, a cellulase, a hemicellulase, a xylase, a mannosidase, a galactosidase, an amylase , disaccharidase, glucose enzyme, glucanase, chitinase, microbial extract enzyme, acid protease, protamex, alkaline protease (alcalase), papain (papain), flavor protease (flavourzyme), Trypsin, bromelain, pepsin, a combination of proteases and isoflavone-degrading enzymes. Next, the hydrolysis step is carried out under the electromagnetic field to obtain a hydrolysis mixture (S240), wherein the hydrolysis step is hydrolyzed with the above enzyme at a temperature of 1 to 100 ° C for 1 second to 96 hours; finally, the hydrolysis mixture is subjected to filtration and deactivation. The final product hydrolysate (S250) was obtained.

First, according to an embodiment of the present invention, an example 1 is provided which will describe the extraction method of the present invention in detail using an example of salmon as an animal biological material.

In this Example 1, the squid was mixed with water at a ratio of 1:0.5 and subjected to a high pressure homogenization process by a homogenizer. It is worth noting that biological raw materials need to be mixed with water to obtain better homogenization effect. However, biological raw materials have a wide range of sources. If the water content of the raw materials is high, it can be directly homogenized without mixing with water. The biological raw material mixture is then divided into four groups, and electromagnetic fields of different voltages are respectively applied thereto, which are (1) 0 V, (2) 1000 V, (3) 3000 V, (4) 6000 V, and (5) 9000 V, respectively. The enzyme used was 0.5% alkaline protein enzyme + 0.5% neutral protein enzyme + 1% flavor enzyme. The flavor enzyme contains endo-enzyme and exo-enzyme, which can remove the bitter peptide of low-water-soluble product at a certain temperature and pH value, completely hydrolyze it to amino acid, and improve the flavor of the hydrolyzate. Enzymes, squid bio-materials After the water is mixed, the bag is sealed, and the hydrolysis step is carried out under the above four electromagnetic fields to obtain a hydrolysis mixture. The hydrolysis step was carried out at 50 ° C for 12 hours.

The five sets of hydrolysis mixture are then heated to deactivate the enzyme, and after cooling through a cooling bath, it is filtered, and then sterilized under high pressure or high temperature to obtain a salmon extract. Filtration can be carried out using a filter aid such as diatomaceous earth or a general filter bag and filter paper to increase the yield.

The results are shown in Table 1, which is the average hydrolyzate peptide concentration in the five groups of salmon extracts under different electromagnetic field conditions. It can be seen from Table 1 that in the case of a DC electromagnetic field, the electromagnetic field is combined with the enzyme, and the average hydrolyzate peptide concentration of the carp is higher than that of the electromagnetic field-free environment. In addition, also in the presence of an electromagnetic field, the higher the electromagnetic field voltage, the higher the average peptide concentration. Therefore, a higher extraction concentration can be obtained with the extraction method disclosed by the present invention.

Further, please refer to Table 2, which is a method for detecting the free amino acid content of the squid obtained by the method of extracting a hydrolyzate according to an embodiment of the present invention using a free amino acid. As can be seen from Table 2, when the traditional simmering method is used for 12 hours, and the general hydrolysis is carried out without the application of an electromagnetic field, the obtained free amino acid content is less than that of applying a 2500 V DC electromagnetic field. The free amino acid content obtained by the method of extracting the hydrolysate. It can be seen from the above that in the embodiment provided by the present invention, the application of an electromagnetic field enhances the extraction efficiency of small molecules such as free amino acids.

Next, the squid peptide obtained by the method for extracting a hydrolyzate of the present invention is further tested for efficacy. Please refer to Table 3, which is a measure of the effect of the squid peptide obtained by the method of extracting hydrolysate according to the embodiment of the present invention on blood pressure lowering by using the efficacy. This test is a test for the inhibition ability of angiotensin converting enzyme (ACE) by using the squid peptide obtained by the method of extracting hydrolyzate in a direct current electromagnetic field environment. As is apparent from Table 3, at the same concentration and the use of the enzyme species, when hydrolyzed by a higher electromagnetic field, the more free amino acid obtained, the more the inhibitory rate of the contained small molecule to ACE is. high. Even in the case of adding a concentration of 5, it is possible to have an inhibition rate of 92%. The method of extracting hydrolysate of the embodiment of the present invention can have the effect of lowering blood pressure.

Next, according to the above-described embodiment of the present invention, an example 2 is provided as follows, which is an example of the extraction method of the present invention by using an example of chicken essence which is generally obtained by concentrating chickens as a biological material.

In this Example 2, the chicken essence was divided into four groups, which were (1) general dropping chicken essence (commercially available), (2) hydrolyzing chicken essence in an electromagnetic field-free environment, and (3) hydrolyzing for 12 hours under an electromagnetic field of 2000 V, And (4) hydrolysis at 2000 V electromagnetic field for 24 hours. The hydrolyzate obtained by the above method for extracting hydrolyzate is tested as a free amino acid content, and refer to Table 4, which is obtained by detecting the method for extracting hydrolyzate according to an embodiment of the present invention by using free amino acid. Chicken sperm free amino acid content. As can be seen from Table 4, when the chicken powder is generally commercially available, and the general hydrolysis of chicken essence is carried out without the application of an electromagnetic field. The free amino acid content is less than the free amino acid content obtained by the method of extracting hydrolyzate applying a direct current electromagnetic field of 2000V. It can be seen from the above that in the embodiment provided by the present invention, the application of an electromagnetic field enhances the extraction efficiency of small molecules such as free amino acids.

Next, according to an embodiment of the present invention, Example 3 is provided as follows, which will describe the extraction method of the present invention in detail using burdock as an example of a plant biological material.

In this example, because the burdock root itself has a high water content, the burdock root is not mixed with water, and is directly homogenized by fruit and vegetable machine. The burdock root homogenate was divided into five groups, one of which was (1) the original burdock homogenate, no enzyme was added, and no electromagnetic field treatment was used as the control group; the other four groups were added 3% fibrinolytic enzyme + 0.5% flavor. The enzymes and the electromagnetic fields to which different voltages are applied are respectively (2) 0V, (3) 500V, (4) 1000V, (5) 2000V. The enzyme and burdock biological raw materials were mixed, bagged and sealed, and hydrolyzed at 45 ° C for 18 hours in the above four electromagnetic fields to obtain a hydrolysis mixture.

Next, the above-mentioned four groups (2), (3), (4), and (5) of the hydrolysis mixture are heated to deactivate the enzyme, cooled by a cooling bath, filtered, and then sterilized under high pressure or high temperature to obtain Burdock root extract. Filtration can be carried out using a filter aid such as diatomaceous earth or a general filter bag and filter paper during the filtration process.

Further, the content of soluble solids (Brix) in the extract of Burdock root obtained by the above five groups of methods was analyzed by a sugar meter. In this example, the active substance to be extracted is exemplified by the content of the soluble solid. Further it may comprise chlorogenic acid or other active substance. The results are shown in Table 5 below, and Table 5 is a test for the properties of the burdock hydrolysate. In Table 2, the soluble solid content extracted from the groups (1) to (5) was about 11%, 12%, 12.8%, 13.3%, and 13.5%, respectively. From this result, it is known that when a pulsed electromagnetic field environment is applied, the extracted active material content is high; and in the case of an electromagnetic field environment, the higher electromagnetic field voltage has a higher extract content. therefore, According to the extraction method of the embodiment of the present invention, the extracted burdock root extract can obtain a higher extraction concentration. In addition, after the extraction hydrolysis mixture is centrifuged at 3000 rpm, the ratio of the obtained supernatant to the original extract can obtain a hydrolysis rate. It can be seen from Table 5 that the hydrolysis rate increases as the electromagnetic field increases. The obtained extract of the burdock root extract was tested for flavor. The original burdock root was only subjected to the homogenization reaction of the fruit and vegetable machine, and there were more fibers and astringent taste; however, the electromagnetic field and the enzyme were added. After multiplication, the cell wall of the root cell of the burdock can be destroyed, and the substance (such as sugar) in the original cell can be dissolved into the liquid, so that it tastes no fiber and has a sweet taste.

Next, according to an embodiment of the present invention, Example 4 is provided as follows, which will describe the extraction method of the present invention in detail using black beans as an example of a plant biological material.

In this example, black beans are mixed with water in a 1:1 ratio. Homogenized by fruit and vegetable machine. The black bean homogenate was divided into four groups, one of which was (1) the original black bean homogenate, without adding any enzyme, and was not treated by electromagnetic field as a control group; the other three groups were added with 3% fibrinolytic enzyme + 1% alkaline. Proteolytic enzyme + 0.5% flavor enzyme, and apply different voltage electromagnetic fields to them respectively (2) 0V, (3) 500V, (4) 2000V. The enzyme and black bean raw materials were mixed, bagged and sealed, and hydrolyzed at 50 ° C for 6 hours in the above three electromagnetic fields to obtain a hydrolysis mixture.

Next, the hydrolysis mixture of the above four groups (2), (3), and (4) is heated to deactivate the enzyme, and after cooling through a cooling bath, filtration, and sterilization at a high pressure or a high temperature to obtain a black bean extract. Filtration can be carried out using a filter aid such as diatomaceous earth or a general filter bag and filter paper during the filtration process.

Further, the content of soluble solids (Brix) in the black bean extract obtained by the above four methods was analyzed by a sugar meter. In this example, the active substance to be extracted is exemplified by the content of the soluble solid. Further it may comprise chlorogenic acid or other active substance. The results are shown in Table 6 below. Table 6 shows the properties of the black bean hydrolysate. In Table 6, the soluble solid content extracted by the groups (1) to (4) was about 9.5%, 12%, 13.5%, and 16.2%, respectively. From this result, it is known that when a pulsed electromagnetic field environment is applied, the extracted active material content is high; and in the case of an electromagnetic field environment, the higher electromagnetic field voltage has a higher extract content. Therefore, according to the extraction method of the embodiment of the present invention, the extracted black bean extract can obtain a higher extraction concentration. In addition, after the extraction hydrolysis mixture is centrifuged at 5000 rpm, the ratio of the obtained supernatant to the original extract can obtain a hydrolysis rate. It can be seen from Table 6 that the hydrolysis rate increases as the electromagnetic field increases. In addition, the obtained black bean extract hydrolyzate was tested for flavor. The original black bean was only subjected to the homogenization reaction of the fruit and vegetable machine, and the fiber residue content was high and the viscosity was sticky; after the electromagnetic field and enzyme multiplication The cell wall of the black bean cells can be destroyed, and the substances (such as sugar) in the original cells can be dissolved into the liquid, so that the taste is lighter and has a sweeter taste.

Next, according to an embodiment of the present invention, Example 5 is provided as follows, which will describe the extraction method of the present invention in detail using kelp as an example of a biological material.

In this example, the kelp is mixed with water at a ratio of 1:10. Homogenized by fruit and vegetable machine. The kelp homogenate was divided into four groups, one of which was (1) the original kelp homogenate, no enzyme was added, and no electromagnetic field treatment was used as a control group; the other three groups were added with 0.5% fibrinolytic enzyme + 1% papain. Enzyme +1% pectinase + 1% xylanase, and respectively applied positive voltage pulse electromagnetic fields of different voltages, respectively (2) 0V, (3) 500V, (4) 2000V. The enzyme and kelp raw materials were mixed, bagged and sealed, and hydrolyzed at 30 ° C for 18 hours in the above three electromagnetic fields to obtain a hydrolysis mixture.

Next, the hydrolysis mixture of the above four groups (2), (3), and (4) is heated to deactivate the enzyme, and after cooling through a cooling bath, filtration, and sterilization at a high pressure or a high temperature to obtain a black bean extract. Filtration can be carried out using a filter aid such as diatomaceous earth or a general filter bag and filter paper during the filtration process.

Further, the content of the soluble solids (Brix) in the kelp extract obtained by the above four methods was analyzed by a sugar meter. In this example, the active substance to be extracted is exemplified by the content of the soluble solid. The results are shown in Table 7 below, and Table 7 shows the properties of the kelp hydrolysate. In Table 7, the soluble solid content extracted by the groups (1) to (4) was about 6%, 7%, 8%, and 8%, respectively. From this result, it is known that when a pulsed electromagnetic field environment is applied, the extracted active material content is high; and in the case of an electromagnetic field environment, the higher electromagnetic field voltage has a higher extract content. Therefore, according to the extraction method of the embodiment of the present invention, the extracted kelp extract can obtain a higher extraction concentration. In addition, the obtained kelp extract hydrolyzate is tested for flavor. The original kelp is only viscous and salty in the hydrolysis mixture obtained after homogenization of the fruit and vegetable machine; after the electromagnetic field and enzyme multiplication, the cell membrane of the kelp cell It can be destroyed, and the substances in the original cells (such as sugar) can be dissolved into the liquid, so it tastes less salty and has a sweeter taste.

In addition to the above examples, in order to further illustrate that the method for extracting hydrolyzate provided by the present invention has unpredictable antibacterial efficacy, the number of bacteria for extracts will be tested below. After the fresh salmon meat was homogenized by homogenizer, it was divided into six groups and placed in a sterile bag, and hydrolyzed by different direct current electromagnetic fields for 24 hours, and the number of bacteria before and after hydrolysis was measured. Please refer to Table 8, which is the bacteriostatic effect of the method for extracting hydrolysate according to the present invention.

As is apparent from Table 8, the number of colonies was greatly reduced after the fresh raw salmon meat was treated for 24 hours using an electromagnetic field as compared with the case where no electromagnetic field was used. Even when an electromagnetic field of 2000V or more is used, the inhibition rate can be as high as 90%, which can greatly inhibit bacterial growth. From this experiment, it can be proved that the method for extracting hydrolyzate provided by the invention can effectively inhibit the growth of microorganisms under a specific electromagnetic field, ensure the occurrence of spoilage during the extraction of the enzyme, and improve the effective concentration and quality of the extraction.

It is worth mentioning that the method for extracting a hydrolyzate provided by the present invention, in addition to utilizing a specific electromagnetic field and an enzyme, may further comprise providing an ultrasonic environment and/or an ultra-high pressure environment to assist in extracting the biological material. Ultrasonic and/or ultra-high pressure can break the biological cell membrane and/or cell wall with high energy, which can increase the efficiency of extracting biological raw materials. However, the invention is not limited thereto.

Accordingly, the method for extracting hydrolyzate disclosed in the present invention utilizes a specific electromagnetic field environment and an enzyme to hydrolyze and extract biological raw materials, and can physically break cells by using physical methods for different biological materials, and then use different types according to the types of biological materials. The specific electromagnetic field and the enzyme are simultaneously subjected to physical and chemical extraction, thereby achieving the effect of effectively extracting the active substances in different biological materials, and obtaining a high concentration of the extract.

As described above, the method for extracting hydrolyzate disclosed by the present invention, combined with electromagnetic field and enzyme extraction, can increase the concentration of the active substance in the hydrolyzed extract, and is more environmentally friendly than the conventional method using an organic solvent.

In conclusion, the present invention has been disclosed in the above preferred embodiments, and is not intended to limit the present invention. A person skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, the scope of the invention is defined by the scope of the appended claims.

S210-S250‧‧‧Steps

Claims (6)

A method for extracting a hydrolysate, comprising: providing a biological material; providing an electromagnetic field to the biological material; and adding an enzyme to the biological material, and performing a hydrolysis step under the electromagnetic field to obtain a hydrolyzate; The biological material comprises an animal, a plant, a microorganism; the electromagnetic field has a voltage of 500 to 10000 V, and the current is between 3 mA and 50 mA, and the electromagnetic field is applied for 6 to 96 hours; the electromagnetic field is a direct current electromagnetic field or a pulse electromagnetic field, and the electromagnetic field is The frequency of the pulsed electromagnetic field is 10 Hz-2000 Hz; and the enzyme is selected from the group consisting of a saccharide-degrading enzyme, a pectinase, a cellulase, a hemicellulase, a xylase, a mannosidase, a galactosidase, an amylase, and a disaccharide. Enzyme, glucose enzyme, glucanase, chitinase, microbial extract enzyme, acid protease, protamex, alkaline protease (alcalase), papain, flavourzyme, trypsin ( One or more of the group consisting of trypsin), bromelain, pepsin, a combination of proteases and isoflavone-degrading enzymes. The method of claim 1, further comprising mixing the biological material with water in a ratio of 1:0.1 to 1:100. The method of claim 1, further comprising heating the biological material. The method of claim 1, wherein the electromagnetic field further comprises Positive high voltage DC electromagnetic field or negative high voltage DC electromagnetic field. The method of claim 1, wherein the hydrolyzing step is carried out at a temperature of from 1 to 80 °C. The method of claim 5, wherein the concentration of the enzyme is from 0.001 to 20% w/v of the total reaction volume.