TWI609631B - Growth regulator composition for improving vegetative growth and reproductive growth of tomato - Google Patents

Abstract

The invention relates to a growth regulating composition for promoting vegetative growth and reproductive growth of tomato, comprising: 10-100 mg/L salicylic acid, 10-100 mg/L auxin, and 0.01-1 mg/L cell division Prime. The growth regulating composition can also promote the nitrogen assimilation enzymes of tomato nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT). active. The invention also relates to a method of using the growth regulating composition to promote vegetative growth and reproductive growth of tomatoes.

Description

Growth regulating composition for promoting vegetative growth and reproductive growth of tomatoes

The invention relates to a growth regulating composition for promoting vegetative growth and reproductive growth of tomatoes, in particular to an enzyme activity for increasing nitrogen assimilation in leaves during vegetative growth, and shortening flowering and fruiting stages and increasing fruit number and quality in reproductive growth. Growth regulating composition.

Small fruit tomato sales in Taiwan account for about 60% of the tomato market, which is a large market. Its characteristics are diverse in shape and color, and can be planted throughout the year. The main planting areas are Yunlin, Chiayi and Tainan. The cultivation area can reach 1,000 to 1,200 hectares (Administration Agricultural Committee, 2010). At present, the source of tomato seedlings in Taiwan includes seed seedlings and grafted seedlings. The excellent seedling status has a significant impact on vegetative growth and early yield from post-planting to flowering. In view of this, the farmers chose to transplant the tomato grafted seedlings seedlings produced by the professional seedling company in a uniform and soil-resistant disease; however, when transplanting to the facility cultivation medium during the four leaf periods of the tomato, the improper transplantation method was often used. Or the difference in the type of medium causes the growth disorder of the root system after transplantation, which in turn affects the absorption of fertilizer by the tomato planting seedlings and the establishment of the seedling biological quality. In severe cases, it even leads to the lack of plants, increasing the subsequent replanting operation and production cost. Therefore, how to improve the growth quality of tomato planting seedlings, and the improvement of agricultural output value can be said to be an urgent matter that is extremely important.

In one aspect, the present invention relates to a growth regulating composition for promoting vegetative growth and reproductive growth of a tomato comprising: 10-100 mg/L of salicylic acid, 10-100 mg/L of auxin, and 0.01-1 mg/L of cells. Mitogen.

In some embodiments, the auxin is indole-3-butyric acid (IBA), indole acetic acid (IAA), naphthaleneacetic acid (NAA) or 2 2,4-Dichlorophenoxyacetic acid (2,4-D); In a preferred embodiment, the auxin is indolebutyric acid (IBA).

In certain embodiments, the cytokinin is 6-Furfurylamino-purine (kinetin), 6-benzyl adenine (BA), Zeatin (Zeatin, ZT) or isopentenylladenine (2ip); in a preferred embodiment, the cytokinin is N6-furanmethyl adenine (kinetin).

In some embodiments, the concentration of salicylic acid is 10-100 mg/L, preferably 10-90 mg/L, more preferably 10-80 mg/L, 10-70 mg/L, 10-60 mg/L, 10-50 mg/L, 10-40 mg/L, 10-30 mg/L, 10-20 mg/L; in a preferred embodiment, the concentration of salicylic acid is 10 mg/L.

In certain embodiments, the concentration of auxin is 10-100 mg/L; in a preferred embodiment, the concentration of auxin is 10 mg/L; in another preferred embodiment, the concentration of auxin is 100mg/L.

In certain embodiments, the concentration of cytokinin is from 0.01 to 1 mg/L; in a preferred embodiment, the concentration of cytokinin is 0.01 mg/L; in another preferred embodiment, cell division The concentration of the prime is 0.1 mg/L; in another preferred embodiment, the concentration of the cytokinin is 1 mg/L.

In another aspect, the present invention relates to a nitrate reductase (NR), a glutamine synthetase (GS), and a glutamate synthase (GOGAT) which promote nitrogen assimilation of tomatoes. An active growth regulating composition comprising: 10-100 mg/L of salicylic acid, 10-100 mg/L of auxin, and 0.01-1 mg/L of cytokinin.

In certain embodiments, the auxin is indole-3-butyric acid (IBA), indole acetic acid (IAA), naphthaleneacetic acid (NAA), or 2,4-dichlorophenoxyacetic acid ( 2,4-D); In a preferred embodiment, the auxin is indolebutyric acid (IBA).

In certain embodiments, the cytokinin is N6-furanmethyl adenine (kinetin), 6-benzyl adenine (BA), zeatin (ZT) or isopentenyl adenine (2ip); In a preferred embodiment, the cytokinin is N6-furanmethyl adenine (kinetin).

In some embodiments, the concentration of salicylic acid is 10-100 mg/L, preferably 10-90 mg/L, more preferably 10-80 mg/L, 10-70 mg/L, 10-60 mg/L, 10-50 mg/L, 10-40 mg/L, 10-30 mg/L, 10-20 mg/L; in a preferred embodiment, the concentration of salicylic acid is 10 mg/L.

In certain embodiments, the concentration of auxin is 10-100 mg/L; in a preferred embodiment, the concentration of auxin is 10 mg/L; in another preferred embodiment, the concentration of auxin is 100mg/L.

In certain embodiments, the concentration of cytokinin is from 0.01 to 1 mg/L; in a preferred embodiment, the concentration of cytokinin is 0.01 mg/L; in another preferred embodiment, cell division The concentration of the prime is 0.1 mg/L; in another preferred embodiment, the concentration of the cytokinin is 1 mg/L.

In still another aspect, the present invention relates to a method for promoting vegetative growth and reproductive growth of a tomato, comprising: applying the above-mentioned growth regulation to promote vegetative growth and reproductive growth of tomato in tomato seedlings. The composition is applied, and the above-mentioned growth regulating composition for promoting vegetative growth and reproductive growth of the tomato is applied to the tomato seedling again after 5-10 days.

In some embodiments, the application is by irrigation and foliar application.

Figure 1 shows the dry weight of the shoots and the dry weight of the lower part of the tomato plants in each treatment group and the control group after treatment of the small-fruit tomato plants with different combinations of growth regulators, respectively, and the negative control group. (Group 7) The multiple of the dry weight of the shoots of the small tomato plants compared to the dry weight of the shoots.

Figure 2 shows the sum of the number of days of flowering and the number of days of fruit in the tomato plants of each treatment group and the control group after treatment of small tomato plants with different combinations of growth regulators.

The invention is further illustrated by the following examples, which are not to be construed as further limiting in any way. The entire contents of all of the cited documents (including references, approved patents, published patent applications, and commonly assigned patent applications in

Unless otherwise defined herein, scientific and technical terms used in connection with this document shall have the meaning as commonly understood by one of ordinary skill in the art. In addition, unless otherwise required by the context, the singular terms shall include the plural, and the plural terms shall include the singular. The methods and techniques of the present invention can generally be carried out according to conventional methods known in the art. In general, the nomenclature described herein to link the following techniques, as well as techniques of biochemistry, enzymology, molecular and cellular biology, microbiology, genetics, and horticulture, are known and often used by the art. . The methods and techniques of the present invention are generally performed according to conventional methods known in the art, and are described in the various general and more specific references that are cited and discussed in this specification, unless otherwise indicated.

As used herein, the term "vegetative growth" means the growth of a plant's vegetative organs, ie roots, stems, leaves, after germination, before flowering. During the vegetative growth period, plants mainly perform photosynthesis and nitrogen assimilation to accumulate carbohydrates and amino acids for later flowering and use. As used herein, the term "promoting tomato vegetative growth" means promoting the growth of a tomato plant during the vegetative growth phase, which may be, but is not limited to, increasing the fresh weight and dry weight of the above-ground and underground parts of the tomato plant, and increasing the chlorophyll of the tomato plant leaves. The content, as well as the enzyme activity of nitrogen assimilation in the leaves of tomato plants.

As used herein, the term "reproductive growth" means the growth of a series of reproductive organs, ie flowers, fruits, seeds, from the beginning of flower bud differentiation, including flower bud differentiation, flowering, pollination, fertilization, fruiting, fruit growth, seed formation. Wait. As used herein, the term "promoting tomato reproductive growth" means promoting the growth of plants and fruits during the reproductive growth period of tomato plants, which may be, but is not limited to, increasing the leaf area of tomato leaves and shortening the vegetative growth period (flowering date). Shorten the result period, increase the flowering rate, increase the number of fruit, increase the number of single fruit, increase the weight of single fruit, increase fruit length, increase fruit width, increase the content of soluble solids in fruits, etc.

As used herein, the term "auxin" means synthesizing at the apical and apical stalks and then transporting them through the stem to various parts of the plant, primarily to promote cell growth, particularly plant elongation and differentiation of plant hormones. Auxin is particularly sensitive to root elongation, but in the prior art, the concentration of auxin used is quite low, about 10 ^-8 M or lower. Higher concentrations of auxin usually promote stem elongation and inhibit roots. Growing. It is known that the application of auxin during the reproductive growth period can promote fruit fruiting, and promotes cell enlargement and elongation, thereby promoting fruit hypertrophy, but application of auxin in the reproductive growth period may have doubts about the application of chemical agents to cause food safety. Auxins include, but are not limited to, indole-3-butyric acid (IBA), indole acetic acid (IAA), naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D). ).

As used herein, the term "cytokinin" means a class of plant growth regulators that promote cell division and differentiation, promote lateral bud growth, and eliminate apical dominance. Cytokinin is an organic molecule with a similar structure. The main synthetic site is the apical meristem, which is sent to the tissues by roots through the xylem, but the root is not the only part of the plant that synthesizes cytokinins, such as maize immature embryos. Cytokinins can be synthesized from developing leaves and young fruits. Cytokinins include, but are not limited to, N6-furanmethyl adenine (kinetin), 6-benzyl adenine (BA), zeatin (ZT), and isopentenyl adenine (2ip).

As used herein, the term "nitrogen assimilation" means nitrate nitrogen (NO ₃ ^- and NO ₂ ^- ) that cannot be directly utilized in plant roots, and is passed through nitrate reductase (NR) and nitrite reduction, respectively. The action of nitrite reductase (NiR) reduces NO ₃ ^- and NO ₂ ^- to ammonium nitrogen (NH ₄ ⁺ ) which can be directly absorbed and utilized by plants, and is accompanied by ammonium assimilation with glutamine synthase (glutamine). Synthetase, GS) works with glutamate synthase (GOGAT) to convert NH ₄ ⁺ into organic amino acids and proteins. Therefore, the activity of nitrogen assimilation enzymes such as nitrate reductase (NR), glutamate synthase (GS) and glutamate synthase (GOGAT) in plant cells increases, indicating that the plant can produce more amines. Acids and proteins.

Example 1 Preparation and Treatment of Test Plants

1. Preparation of test plants

The test plants used in the present example are small fruit tomato plants; the plug seedlings of the small fruit tomato grafted seedlings were purchased from Jiahua Seedling Nursery Field (Taiwan) in Chiayi County, and the small fruit tomato plant scion varieties of the small fruit tomato grafted seedlings were saints. (Santa), the rootstock is FondMay eggplant.

2. Planting and processing methods of test plants

The test period is between the seedling stage of the test plant and the result period. The seedlings of the small fruit tomato grafted seedlings purchased from the seedling farm were domesticated for 1 week, and the neat and consistent small fruit tomato grafted seedlings were selected. The seedlings were transplanted, and the peat soil and the pearl stone were mixed at a ratio of 3:1 as a cultivation medium. The plug seedlings were transplanted into a 3.5-inch plastic pot to become a fixed seedling of the grafted seedlings of the small fruit tomato. The growth regulating composition of the present invention was treated by the root watering method, and as shown in Table 1, each treatment was repeated 3 times, and the growth regulating composition of the present invention (50 mL/plant) was once poured once a week for co-treatment. 2 times; after transplanting, diluted 1000 times per week with Huabao 2 (nitrogen-phosphorus-potassium ratio 20-20-20) as top dressing, and the test plants were changed to 7-inch pots before flowering, after flowering period Each 2-3 weeks was diluted 1,500 times with Taifei No. 43 and applied as a fertilizer (200 mL/plant), and the growth and development of the test plants were continuously observed until the fruit quality was investigated in the results.

Effects of growth of growth regulating composition of the embodiment of the invention, two small raised Tomato plants camp

For the test plants which were treated for 2 weeks after the treatment with the growth regulating composition of the present invention in Example 1, the growth and development parameters related to the vegetative growth period were analyzed, including: the stem diameter, the plant height, the shoot and the fresh ground of the test plant. Heavy and dry weight, 3 repetitions per treatment. Dry weight in the ground and below After harvesting the fresh and small fruit tomato plants, after drying for 3 to 4 days at 70 ° C, the dry weight of the shoots and the lower part of the ground was measured.

Data analysis and statistical methods

The values measured in each batch were analyzed by Student's T-test after taking 3 biological replicates and standard deviations, and were considered to be significantly different at p < 0.05. In addition, the value of each treatment group was divided with the negative control group (Group 7, that is, the group in which no growth regulator was not treated), standardized, and compared in a magnification presentation manner.

The results are shown in Table 2. Compared to the negative control group (Group 7, that is, the group in which no growth regulator was not treated), the growth regulating composition of the present invention grew for the vegetative phase of the small tomato plant, including above ground. The fresh weight of the part and the lower part of the ground, the dry weight of the aboveground part and the lower part of the ground have no inhibitory effect (there is no statistically significant difference between the results of the negative control group) and even the effect of promoting the growth of the small-fruit tomato plant during the vegetative period; for example, this The first group of growth regulating compositions of the invention can promote the fresh weight, the aboveground and the lower part of the dry weight of the aboveground and lower parts of the small tomato plants, and have statistically significant differences compared with the negative control group. In contrast, the control group had no such effect on the growth of the vegetative phase of the small-fruit tomato plants; for example, although the 13th group could increase the fresh weight of the small-sized tomato plants, it was aboveground and underground. The dry weight of the part has a restraining effect.

As shown in Fig. 1, while comparing the dry weight of the aboveground and underground parts of the vegetative period of the small tomato plants, it can be seen that the growth regulating composition of the present invention can promote the growth of the vegetative period of the small tomato plants compared with the negative control group, and The development trend of the aboveground and underground parts is the same.

Chlorophyll content of growth regulator composition of the invention three embodiments of the small fruit of tomato plants leaves nutrition

Photosynthesis is the main source of nutrients for plants. The most important element of plant photosynthesis is chlorophyll. The chlorophyll of higher plants is divided into two types: chlorophyll a (Chl a) and chlorophyll b (Chl b), Chl a and Chl. The increase in b content can greatly increase the utilization of light energy by plants. The chlorophyll a/b ratio (Chl a/b) of most healthy plants is more than 3. In this example, the test plants were tested for chlorophyll content.

For the test plants which were treated for 2 weeks after the treatment with the growth regulating composition of the present invention in Example 1, 30 mg of the leaves of the complete leaves of the tomato were added, 1 mL of 80% acetone was added to extract the chlorophyll, and the leaf tissue was ground and then After standing at 4 ° C in the dark, the supernatant was centrifuged, and the absorbance at 645 nm and 663 nm was measured using a multi-function microplate reader to convert the chlorophyll content (chlorophyll a and chlorophyll b, respectively). 3 repeat. The data analysis and statistical methods are as described in Example 2.

The results are shown in Table 3. Compared to the negative control group (Group 7, that is, the group in which no growth regulator was not treated), the tomato plants treated with the growth regulating composition of the present invention had vegetative leaves. The chlorophyll content has an increasing trend, although there is no statistically significant difference. In contrast, the control group had no such effect on the growth of vegetative growth of small tomato plants; for example, although the 11th group increased the chlorophyll content of the vegetative leaves of small tomato plants, the 9th and 13th The group will cause a decrease in the chlorophyll content of the leaves of the small tomato plants during the vegetative stage. On the chlorophyll a/b ratio, the negative control group (Group 7) was 2.67, and the chlorophyll a/b ratio of the small tomato plants treated with the growth regulating composition of the present invention was higher than that of the negative control group, and even reached 3 The above shows that the growth regulating composition of the present invention has no side effect on the vegetative growth of tomato plants, and the tomato plants treated with the growth regulating composition of the present invention are in a healthy state.

Effect of enzyme activity assimilation growth regulating composition of the invention four embodiments of support of the smaller nitrogen Tomato plants camp

For the test plants which were treated for 2 weeks after the treatment with the growth regulating composition of the present invention in Example 1, the activity of the nitrogen assimilation enzyme of the newly grown complete leaves was detected by a multi-function microplate reader, and the nitrogen assimilation was detected. Enzymes include nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT).

Nitrate reductase (NR) activity assay According to Gao et al. (2013) analysis, 0.5 g of fresh leaves were weighed and 1 mL containing 7.5 mM cysteine (cysteine), 1 mM EDTA, 1.5% casein was added. Potassium phosphate buffer (100 mM, pH=7.4), and added with liquid nitrogen, and centrifuged at 13,000 relative centrifugal field (rcf) for 30 minutes at 4 ° C, then 0.1 mL of supernatant was added. After reacting 0.9 mL of the reaction solution in a 30 ° C water bath for 30 minutes, the reaction was terminated by adding 0.05 mL of zinc acetate (1 M), and the supernatant was centrifuged at 3,000 rcf at room temperature, and 0.5 mL of acesulfame was added. The amine (sulphanilamide, 5.8 mM) and 0.5 mL of N-(1-naphthylethylenediamine, 0.8 mM) were uniformly mixed and allowed to stand for 30 min, and the absorbance at a wavelength of 540 nm was measured. One nitrate reductase (NR) activity unit is defined as the amount of enzyme required to metabolize 1 nmole of NO ₂ ^{- in} 1 minute, in units of nmol NO ₂ ^- . g ^-1 FW.

The analysis of branide synthase (GS) activity was carried out according to the method of Oak et al. (1980). 0.5 g of the leaves were placed in a mortar, and a small amount of liquid nitrogen was added to the powder, and 10 mM Tris-HCl (pH=) was added. 7.6), 1 mM extract of 1 mM magnesium chloride (MgCl ₂ ), 1 mM EDTA and 10 mM 2-mercaptoethanol, centrifuged at 13,000 rcf for 30 minutes at 4 ° C, and 0.1 mL of the supernatant was added to 0.4 mL of the reaction solution. After reacting for 30 minutes in a water bath at 30 ° C, the reaction was terminated by adding 1 mL of a reaction stop solution containing 2.5 g of ferric chloride (FeCl ₃ ), 5.0 g of TCA dissolved in 100 mL of hydrogen chloride (HCl, 1.5 N), and 3,000 rcf at room temperature. After centrifugation, the supernatant was taken, and the absorbance was measured at a wavelength of 540 nm. One glutamate synthase (GS) activity unit is defined as the amount of enzyme required to catalyze the production of 1 μmol of L-Glutamic acid γ-monohydroxamate at 30 ° C in 1 minute. The unit is μmol γ-GMH. g ^-1 FW.

The activity analysis of glutamate synthetase (GOGAT) was carried out according to the method of Lin et al. (2000). 0.5 g of leaves were placed in a mortar, and a small amount of liquid nitrogen was added to the powder, and 10 mM Tris-HCl (pH=7.6) was added. 1 mL of 1 mM MgCl ₂ , 1 mM EDTA and 10 mM 2-Mercaptoethanol was centrifuged at 13,000 rcf for 30 minutes at 4 ° C, and 0.15 mL of the supernatant was added to contain 0.2 mL of 20 mM L-glutamine. The reaction was initiated with a reaction solution of 0.25 mL of 2 mM ketoglutarate, 0.05 mL of 10 mM potassium chloride (KCl), 1 mL of 25 mM Tris-HCl (pH = 7.6), and 0.1 mL of 3 mM NADH, and the measurement was performed after excitation at a wavelength of 340 nm. The reading is received at a wavelength of 445 nm. The amount of enzyme required to reduce 1 μmol of NADH in 1 minute of the reaction solution was defined as one glutamate synthetase (GOGAT) enzyme activity unit. Total glutamate synthetase (GOGAT) activity is expressed as μmol of NADH reduction per gram of fresh weight material per minute, and its unit is μmole NADH oxidized. Min ^-1 . g ^-1 FW.

The results are shown in Table 4. Compared with the negative control group (Group 7, that is, the group in which no growth regulator was not treated), the nitrogen-assimilation of the growth regulating composition of the present invention on the vegetative leaves of the small tomato plants The activities of enzymes, including nitrate reductase (NR), glutamate synthase (GS), and glutamate synthase (GOGAT), have a tendency to promote the activity of these enzymes, and some even have statistically significant differences. For example, the second group growth regulating composition of the present invention can promote the activities of nitrate reductase (NR) and glutamate synthase (GS) in the leaves of small tomato plants, and has statistics compared with the negative control group. Significant difference on. In contrast, the effect of the control group on the activity of nitrogen assimilation enzymes in the vegetative stage of small tomato plants did not have such a consistent effect; for example, although the ninth group could increase the glutamic acid in the leaves of small tomato plants Synthetic enzyme (GS) has an inhibitory effect on its nitrate reductase (NR).

Effect of reproductive traits growth regulating composition 5 of the present invention embodiments of Coffea green tomato plants

For the test plants treated with the growth regulating composition of the present invention in Example 1, the growth and growth parameters related to the reproductive growth period were analyzed, including: leaf area in the result period, number of days of flowering, number of days of the result, single fruit The number, single fruit weight, fruit length, fruit width, and soluble solids contained in the fruit, etc., were repeated 3 times for each treatment. The investigation is as follows: (1) Leaf area in the result period: Leaf area parameters of the fruit tomato plants after treatment with the growth regulating composition of the present invention were obtained by using a leaf analysis system (WinFOLIA Rro LA2400, Regent); (2) Number of flower days: the number of days required for flowering of the first inflorescence of 50% of plants after planting (only one day, no statistical analysis); (3) Flowering rate: percentage of flowering of a bunch of inflorescences; (4) Results day Number: the number of days required for the first inflorescence of 50% of plants after planting (only one day, no statistical analysis); (5) Number of fruits: percentage of a series of inflorescence results of plants; (6) fruit traits Survey: measuring the fruit weight, fruit number, fruit length and fruit width of the second fruit of the plant; fresh fruit weight The scales and fruit widths were measured and measured using a vernier caliper; (7) Fruit quality survey: analysis of the soluble solids of the fruit. The fruit was stalked, the tomato juice was collected by juice extraction, and the pomace was filtered through a sieve. 0.2 mL of the supernatant was taken and the oBrix value was measured on a sugar acidity meter (Atago Digital Refractometer PAL-BX/ACID3) to determine the soluble solid matter. The content.

The results are shown in Tables 5, 6, and 7. Compared with the negative control group (Group 7, that is, the group in which no growth regulator is not treated), the growth regulating composition of the present invention has a tendency to increase the leaf area of the small tomato plants, and some groups even have Statistically significant differences (as shown in Table 5). In addition, the growth regulating composition of the present invention has a tendency to promote early flowering and fruiting of small-fruit tomato plants, as shown in Table 5 and Figure 2 (please provide), compared to the negative control group (Group 7), the present invention The growth regulating composition can shorten the vegetative growth period of the small-fruit tomato plants, make the small-fruit tomato early, and shorten the time of the small-fruit tomato, so that the small-fruit tomato can be harvested early. Further, in the tomato plant treated with the growth regulating composition of the present invention, the flowering number and the fruiting rate were not reduced by the shortening of the vegetative growth period, as shown in Table 5, compared with the negative control group (Group 7). The growth regulating composition of the present invention has a tendency to increase the flowering rate and the number of fruit of the small tomato plant; in addition, as shown in Table 6, the growth regulating composition of the present invention also increases the number of single fruit of the small tomato plant. There was a statistically significant difference compared to the negative control group (Group 7). From this, it is understood that the growth regulating composition of the present invention can shorten the vegetative growth period of the tomato, allow it to be harvested early, and increase the number of results.

More importantly, shortening the fruit quality of small fruit tomatoes in the vegetative growth period has not been affected. As shown in Table 6, compared to the small control tomato of the negative control group (Group 7), the single fruit weight of the small fruit tomato treated with the growth regulating composition of the present invention increased, and some even had statistically significant differences; In terms of fruit size, the growth-regulating composition of the present invention also had a tendency to increase the fruit length and fruit width of the small fruit tomato compared to the negative control group (Group 7), and some even had statistically significant differences. Further, as shown in Table 7, the soluble solids of the small fruit tomato treated with the growth regulating composition of the present invention was compared with the negative control group (Group 7). The content of substances (carbohydrate) has an increasing trend, and some even have statistically significant differences. For example, in comparison with the negative control group (Group 7), the fifth group growth regulating composition of the present invention can promote the leaf area of the leaves of the small fruit tomato plants (as shown in Table 5), and the vegetative growth period (starting) The number of flower days and the number of results were shortened by 8 days (23-15 days) and 14 days (44-30 days), respectively (as shown in Table 5), and there was also a negative rate of flowering rate and result rate. In the control group (Group 7), the increase in the number of single fruit, single fruit weight, fruit length and fruit width was higher than that of the negative control group (Group 7), and there was a statistically significant difference, and the fifth group produced The soluble solids content of the small fruit tomato was also significantly higher than that of the negative control group (Group 7). In contrast, the control group had no such consistent effect on the reproductive growth period of the small-fruit tomato plants; for example, compared with the negative control group (Group 7), the eighth group could shorten the nutrition of the small-fruit tomato plants. Growth period (number of days of flowering) and number of days of the result, and there is a tendency to increase the leaf area and flowering rate of the plant at the end of the plant (as shown in Table 5), and can also significantly increase the number of single fruit, but in the later stage of the result, Eight groups of small fruit tomato plants began to die, and no fruit was harvested (as shown in Tables 6 and 7). For example, the leaf leaf area, single-strand fruit number, single fruit weight, fruit length, fruit width, and fruit soluble solid content of the 10th group of small-fruit tomato plants increased compared with the negative control group (Group 7). However, it does not shorten the vegetative growth period and the number of days of fruit tomato, and cannot achieve the advantages of adjustment of production period and reduction of production management costs.

It can be seen from the results of the above various examples that the growth regulating composition of the present invention can simultaneously promote the vegetative growth and reproductive growth of tomato plants, especially in increasing the enzyme activity of nitrogen assimilation in the vegetative growth period, and shortening the vegetative growth in reproductive growth. The period and the result period and the increase in the number of fruits, the size of the fruit, and the quality of the fruit have a significant promoting effect relative to the tomato plants which have not been treated with the growth regulator. More importantly, as long as the growth regulating composition of the present invention is applied twice in the seedling stage, it is not necessary to apply any growth regulating agent at the flowering stage or the fruiting stage, thereby achieving the effect of the above-mentioned early flowering result harvesting, not only application. Convenient, it can also avoid the doubts about food safety during the harvest period. Therefore, the growth regulating composition of the invention can shorten the tomato planting time, reduce the production management cost, and has the advantages of adjusting the production period and improving the farmers' cultivation profit.

The detailed description of the preferred embodiments of the present invention is intended to be limited to the scope of the invention, and is not intended to limit the scope of the invention. The patent scope of this case.

In summary, the technical features disclosed in this case have fully complied with the statutory invention patent requirements of novelty and progressiveness. If you apply in accordance with the law, you are requested to approve the application for this invention patent to encourage invention.

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Claims (8)

A growth regulating composition for promoting vegetative growth and reproductive growth of a tomato comprising: 10-100 mg/L of salicylic acid; 10-100 mg/L of auxin; and 0.01-1 mg/L of cytokinin. The composition of claim 1, wherein the auxin is indole-3-butyric acid (IBA). The composition of claim 1, wherein the cytokinin is N6-Furfurylamino-purine. A growth regulating composition for promoting nitrogen assimilation of tomato enzymes nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT) activity, Contains: 10-100 mg/L salicylic acid; 10-100 mg/L auxin; and 0.01-1 mg/L cytokinin. The composition of claim 4, wherein the auxin is indole-3-butyric acid (IBA). The composition of claim 4, wherein the cytokinin is N6-Furfurylamino-purine. A method for promoting vegetative growth and reproductive growth of a tomato, comprising: applying the composition as claimed in claim 1 to the planted tomato seedling; and applying the tomato seedling again after 5-10 days as claimed The composition of item 1. The method of claim 7, wherein the method of application is a watering method.