TWI603732B - Contains sesamin-class and γ-furfuryl alcohol and room germ oil composition - Google Patents

Description

Composition containing sesamin and gamma-mysterol and rice germ oil

The present invention relates to an in vivo absorption enhancer for sesamin, and a composition using the food or drink, a pharmaceutical composition or the like. In addition, the invention also relates to the use of the composition as an antioxidant.

Sesamin is one of the lignan compounds contained in sesame. Among them, sesamin with respect to sesamin and its stereoisomers is known to have a role in lowering blood cholesterol, lowering blood neutral lipids, improving liver function, removing reactive oxygen species, and inhibiting Δ5 desaturase. It inhibits various physiological activities such as a lipid peroxidation, an antihypertensive action, a detoxification prevention, and a breast cancer action (Patent Document 1).

However, the lignan compound containing sesamin is only soluble in water, and it can only be dissolved to some extent in an organic solvent which can be used for medical use or consumption. Due to such poor solubility, there is a problem that lignan compounds are difficult to be absorbed in the living body.

A method of improving the in vivo absorbability of a lignan compound containing a sesamin-based compound has been proposed, for example, by a method of emulsification (Patent Documents 1 and 2). However, a simpler method, such as by simply combining with other compounds Reports of in vivo absorption of high sesamin are rare.

On the other hand, a composition containing sesamin and γ -mysterol is known (Patent Document 3). Further, a composition containing sesamin and rice germ oil is also known (Patent Document 4). However, it has not been known whether these compositions can increase the in vivo absorption of sesamin.

Patent Document 1: WO2006/106926

Patent Document 2: WO2008/044550

Patent Document 3: JP-A-2009-73749

Patent Document 4: WO2007/037385

In order to form an emulsified form for the purpose of improving absorption, it is necessary to uniformly emulsify a fat-soluble substance, and since it is necessary to carry out complicated steps, there is a problem associated with an increase in cost. An object of the present invention is to provide a novel method which can solve such problems and which aims to promote absorption of sesamin in the body.

In order to solve the above problems, the team of the present invention found that when sesamin, gamma -mysterol, and rice germ oil are used in combination, the in vivo absorption of sesamin can be promoted. The effect is particularly remarkable when γ -methanol and rice germ oil are used in a specific ratio.

Therefore, the present invention includes the following contents, but is not limited thereto.

1. A composition, which system contains at least one sesamin-class compound, at least one of γ - m furfuryl alcohol, and the composition of the rice germ oil, wherein: the γ - m on the total weight of the furfuryl alcohol :( γ - m furfuryl alcohol The total weight is the sum of the weight of the rice germ oil) = 1:4~1:66.

2. The composition of 1, wherein the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the alcohol with the total amount of rice germ by weight of the oil) = 1:20 to 1: 60

.. 2A The composition of 2, wherein the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the alcohol and the total amount of the rice germ oil by weight) = 1: 25 to 1:55.

. The compositions 2b 2a of which the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the alcohol with the total amount of rice germ by weight of the oil) = 1: 32 to 1:54.

3. The composition according to any one of items 1 to 2, wherein, when the total weight of the sesamin-class is 1, the total weight of the gamma -mysterol is 0.3 or more.

4. The composition according to any one of items 1 to 3, wherein the sesamin-class is sesamin and/or episesamin.

5. A composition according to any one of items 1 to 4 which is used orally.

6. The composition according to any one of items 1 to 5, which is a food or drink.

7. The composition of any one of items 1 to 6, which is an antioxidant.

8. An in vivo absorption promoting agent which promotes in vivo absorption of at least one sesamin, which comprises at least one gamma -mysterol and rice germ oil as an active ingredient.

9. Use of a composition comprising at least one gamma -mysterol and rice germ oil as an active ingredient for the manufacture of at least one sesamin-promoting in vivo absorbent.

10. The promotion of 8 or 9 of the absorbent body, wherein the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the furfuryl alcohol and the weight of rice germ oil total amount) = 1: 4 to 1 :66.

11. The absorbent body of promoting or 10, wherein the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the alcohol with the total amount of rice germ by weight of the oil) = 1: 20 ~ one sixty .

11a of absorber 11 such as the promotion or in vivo, wherein the γ - :( total weight of the furfuryl alcohol meters γ - the total weight of the rice and the rice germ furfuryl alcohol by weight of the total amount of oil) = 1: 25 to 1:55 .

11a 11b The use of absorbers or promote in vivo, wherein the γ - :( total weight of the furfuryl alcohol meters γ - m on the total weight of the total amount of alcohol and the weight of the oil of the rice germ) = 1: 32 to 1:54 .

12. The in-vivo absorbent or use according to any one of items 8 to 11b, wherein the total weight of the sesame-containing sterol is 1 or more, and the total weight of the gamma -mysterol is 0.3 or more.

13. The in vivo absorption or use according to any one of items 8 to 12, wherein the sesamin-class is sesamin and/or episesamin.

14. The in vivo absorption or use of an absorbent according to any one of clauses 8 to 13, wherein the aforementioned in vivo absorption of the absorbent is oral.

15. The composition according to any one of items 1 to 7, wherein the total amount of sesamin is 1 w/w% or more based on the total amount of the composition.

16. A method for promoting in vivo absorption of at least one sesamin, characterized in that the method comprises administering the sesamin, at least one gamma -mysterol, and rice germ oil.

According to the present invention, when sesamin, γ -mysterol, and rice germ oil are used in combination, the in vivo absorption of sesamin can be improved. This effect is particularly remarkable when the amount of the latter two components is used in a specific ratio. Therefore, the dosage of sesamin can be increased without increasing the physiological activity. Further, the present invention can also remarkably enhance the antioxidant action of a composition comprising sesamin, γ -mysterol and rice germ oil. Therefore, the present invention also provides an antioxidant which can exert an excellent antioxidant action.

In addition, γ -mysterol is known to have various physiological effects such as lowering the effect of neutral lipids in the blood, regulating endocrine/autonomic nerve action, and protecting the gastric mucosa. Further, since sesamin and γ -methanol are derived from plants, they are extremely safe. Therefore, the present invention can not only improve the absorbability of sesamin, but also an effective physiological action of γ -methanol, and can provide a food or drink and a pharmaceutical composition which can be safely and continuously ingested.

Fig. 1 is a view showing the amount of sesamin and episesamin absorbed in the intestinal tract.

Fig. 2 is a view showing an AUC of a ΔORAC value.

Fig. 3 is a view showing an AUC of a ΔORAC value.

(sesame)

The sesamin-class of the present invention is a generic term for all compounds including sesamin and its isoforms. In addition to the episesamin, the above-mentioned sesamin-based isoforms may, for example, be a dioxabicyclo[3.3.0]octane derivative described in JP-A-4-9331. Specific examples of the sesamin are sesamin, sesame alcohol, epiformyl alcohol, sesaminol, and the like, and stereoisomers or optical isomers thereof may be used singly or in combination, and in the present invention, sesame is used. It is better to use vegetarian and/or episesamin. In addition, the sesamin-like metabolites (for example, those disclosed in JP-A-2001-139579) may be used as the sesamin-like isoforms of the sesamin-containing compounds of the present invention within the range in which the effects of the present invention are exhibited. And used in the present invention.

The sesamin used in the present invention is not limited by the form, the production method, and the like. For example, when sesamin is used as the sesamin, a sesamin extract (called a sesamin extract or concentrate) extracted from a sesame oil by a known method (for example, the method described in JP-A-4-9331) can be used. ), or directly use commercially available sesame oil (liquid). However, when sesame oil is used, since the content of sesamin is extremely low (generally less than 1%), when the desired sesamin is to be blended in order to obtain the physiological action of sesamin, the volume of the unit dose of the formulated composition is too large. Will cause difficulty in ingestion. In particular, after formulation for oral administration, there is an obstacle to ingestion due to an excessively large amount of the preparation (tablet, capsule, etc.). Therefore, it is preferable to use a sesamin extract (or sesamin concentrate) derived from sesame oil from the viewpoint that the amount of intake is small. In addition, due to the unique flavor of sesame oil, poor sensory evaluation may be obtained, and the sesamin extract may be obtained by a known method such as active clay treatment. (or sesamin concentrate) for odorless and odorless treatment.

Therefore, the sesamin-based concentrate is preferably obtained by extracting and/or purifying the obtained sesamin-containing material from a food material, and then obtaining the sesamin-containing concentrate having an increased concentration. The degree of concentration can be appropriately set depending on the type of sesamin used and the form of the blended composition, and generally, a sesamin concentrate obtained by concentrating the total amount of sesamin into 1 w/w% or more is used. good. The total sesamin content in the sesamin concentrate is preferably 20 w/w% or more, more preferably 50 w/w% or more, 70 w/w% or more, and further preferably 90 w/w% or more.

The content of the sesamin can be measured by any one of known methods such as HPLC. For the HPLC method, for example, the method described in JP-A-2009-155312, and the like can be referred to.

( γ -mysterol and rice germ oil)

One of the active ingredients which can promote the absorption of sesamin in the body is γ -methanol. It is known that γ -methanol has a role in lowering neutral lipids in the blood (Geriant. med. 19, 1812-1840, 1981) and regulating endocrine/autonomic nervous effects (Japanese Medicine, 75(4), 399-403, 1979). It protects the gastric mucosa (Japanese Medicine, 84 (6), 537-542, 1984) and other physiological effects. It has been approved as a therapeutic agent for hyperlipemia and psychosis (menopausal disorders, allergic bowel irritation). Medicines such as therapeutic agents. The γ -mysterol used in the present invention is a generic term for a combination of ferulic acid ester bond with triterpene alcohol or sterol. Examples of the triterpene alcohol include cyclocarenol, 24-methylenecycloartanol, cycloartanol, cyclodextrol, and the like. The sterol may, for example, be vegetable sterol or soybean solid. Alcohol, β-steroids, and the like. In the present invention, these compounds may be used singly or in the form of a mixture. As a method of quantifying γ -methanol, a well-known method such as a UV absorbance method or an HPLC method can be suitably used. For example, as described in "Standards for Pharmaceutical Raw Materials" and "Independent Specifications for Food Additives Other than Chemically Synthetic Products", the sample is dissolved in heptane and used to measure the absorbance at a maximum wavelength absorbed near 315 nm. Can be quantified.

It is known that brown rice, corn, barley, and the like contain γ -methanol. Gamma -mysterol is also contained in rice bran oil from the oil extracted from brown rice.

Further, γ -methanol is also contained in rice germ oil which is one of active ingredients which promotes absorption of sesamin in the body. The rice germ oil is a fat extracted from the germ of rice. It is generally considered that the γ -mysterol contained in the rice germ oil is about 1.0 to 1.5 w/w% (this concentration is the total amount of rice germ oil containing the weight of γ -methanol). The weight is standard). The content of the rice germ oil can be measured by any of the known methods. For example, the content of the rice germ oil can be measured by using the content of the sterol which is peculiar to the rice germ oil or the rice germ oil concentrate, and the content ratio of various sterols as an index. Specifically, since rice germ oil has a large amount of bound sterol compared with free sterol, it can be judged whether or not a certain composition contains rice germ oil based on the sterol composition. In addition, since the content of the vegetable oil sterol and the soybean sterol in the rice germ oil is high, the content of these may be used as an index for determining the content of the rice germ oil.

The γ -mysterol and rice germ oil used in the present invention are not limited by the source, form, production method and the like.

For example, γ -mysterol can be directly used as a commercially available rice germ oil, but as described above, the amount of γ -mysterol contained in the rice germ oil is not high. Therefore, in order to blend γ -mysterol and rice germ oil in the ratio described later, it is not sufficient to merely blend the two. Accordingly, therefore, the two kinds of substituted or added in the form of using gamma] - meters high alcohol content is preferred, for example using gamma] from rice bran and rice germ oil - A furfuryl alcohol rice concentrate better. When the γ -mysterol concentrate is used, the γ -mysterol content in the γ -mysterol concentrate is preferably 2 w/w% or more in terms of the total amount, more preferably 30 w/w% or more (these concentrations are Based on the total weight of the concentrate containing gamma -mysterol. The γ -mysterol concentrate can be obtained by industrially extracting from rice bran oil and rice germ oil using an organic solvent, or after purification by treatment with a resin, for example, rice germ oil γ 30 can be purchased. Food Industry Co., Ltd.), γ -methanol, Orizadorimu V-50, rice bran oil, brown rice germ oil (ORYZA Oil Chemical Co., Ltd.).

Rice germ oil is currently used, for example, containing 1.2 to 1.5% of γ -mysterol (this concentration is based on the total weight of rice germ oil containing γ -mysterol) ORYZA Oil S-1 (Yumaru Natural Beauty Co., Ltd.) Having a 1.0-1.5% γ -mycohol (this concentration is based on the total weight of rice germ oil containing γ -mysterol), such as rice germ oil PRO-15 (Tsino Food Industry Co., Ltd.) It is sold in the market.

(Constituents containing sesamin and γ -mysterol and rice germ oil)

According to the present invention, when sesamin, gamma -mysterol, and rice germ oil are used in combination, it is possible to improve the in vivo absorption of sesamin. Therefore, the physiological activity of each component can be exhibited efficiently, and the effect of improving health can be expected by the physiological action of each component.

The blending amount and blending ratio of each component in the composition (food and beverage, pharmaceutical composition, etc.) of the sesamin-containing, gamma -mysterol, and rice germ oil of the present invention can promote sesamin as described later. It is preferred that the absorption in the body can be appropriately selected within the range in which physiological activity can be exerted efficiently.

In the composition of the present invention, when it is expected to promote the in vivo absorption effect of sesamin, the total weight of γ -mysterol contained is the total amount of γ -mysterol and the weight of the rice germ oil. Proportion, preferably 1:4~1:66, 1:8.7~1:62, 1:20~1:60 is better, 1:25~1:55, 1:32~1:54 or 1:32.4 ~53.2, better system 1:32~1:49. Further, when the rice germ oil to be used contains γ -mysterol, the weight of the rice germ oil described in the present invention is not in the same range as the weight of the γ -mysterol, and means The weight of γ -methanol was removed from the total amount.

Further, when the total weight of the sesamin-containing compound is 1, the total weight of γ -methanol is preferably 0.3 or more, more preferably 0.4 or more, 0.6 or more, 0.69 or more, 0.8 or more, and more preferably 1.0 or more. The upper limit of the value is not particularly limited, and may be, for example, 5.0 or less, more preferably 3.0 or less, 1.6 or less, 1.5 or less, 1.4 or less, 1.39 or less, 1.2 or less, 1.1 or less, and still more preferably 1.06 or less. The invention includes all combinations of such upper and lower limits. When the total weight of the sesamin-class is 1, the preferred total weight range of γ-mysterol is as follows: 0.3 to 5.0, 0.3 to 3.0, 0.3 to 1.6, 0.3 to 1.5, 0.3 to 1.4, 0.3 to 1.39, 0.3~1.2, 0.3~1.1, 0.3~1.06; 0.4~5.0, 0.4~3.0, 0.4~1.6, 0.4~1.5, 0.4~1.4, 0.4~1.39, 0.4~1.2, 0.4~1.1, 0.4~1.06; 0.6~ 5.0, 0.6~3.0, 0.6~1.6, 0.6~1.5, 0.6~1.4, 0.6~1.39, 0.6~1.2, 0.6~1.1, 0.6~1.06; 0.69~5.0, 0.69~3.0, 0.69~1.6, 0.69~1.5, 0.69~1.4, 0.69~1.39, 0.69~1.2, 0.69~1.1, 0.69~1.06; 0.8~5.0, 0.8~3.0, 0.8~1.6, 0.8~1.5, 0.8~1.4, 0.8~1.39, 0.8~1.2, 0.8~ 1.1, 0.8~1.06; 1.0~5.0, 1.0~3.0, 1.0~1.6, 1.0~1.5, 1.0~1.4, 1.0~1.39, 1.0~1.2, 1.0~1.1, 1.0~1.06. A particularly suitable range is, for example, 0.69 to 1.39, 0.69 to 1.06.

Regarding the blending amount, the amount of the sesamin compound which can be blended in the composition of the present invention is preferably from 1 to 100 mg, more preferably from 1 to 50 mg, and most preferably from 3 to 30 mg. Γ -methanol can be blended with 1~100mg, preferably 1~60mg, and 3~30mg. Rice germ oil is best blended with 1~5000mg, 10~1000mg, and 30~500mg.

The total blending ratio of sesamin in the composition of the present invention is preferably 1 w/w% or more, more preferably 1 to 50 w/w%, and most preferably 1 to 10 w/w%, regardless of the total weight of the composition. Further, the typical total blending ratio of γ -mysterol is preferably 0.1 to 50 w/w%, more preferably 0.5 to 30 w/w%, and most preferably 1 to 10 w/w%, based on the total amount of the composition. The typical total blending ratio of rice germ oil is 5~99w/w%, 20~98w/w% is better, and 50~95w/w% is the best relative to the total amount of the composition.

Further, when the form of the composition of the present invention is a liquid preparation, a typical total blending ratio of sesamin is 0.001 w/w% or more, more preferably 0.001 to 0.5 w/w%, and most preferably 0.001 to 0.05 w/w%. The typical total blending ratio of γ -methanol is 0.0001~0.1w/w%, 0.0005~0.05w/w% is better, 0.001~0.02w/w% is best, and rice germ oil is typical. The total blending ratio is preferably 0.05 to 5 w/w%, more preferably 0.1 to 2.5 w/w%, and most preferably 0.2 to 1 w/w%, relative to the total amount of the composition.

The form and the ingestion route of the composition of the present invention are not particularly limited. However, in the present invention, it is preferred to take it in the form of an oral composition when it is ingested for the purpose of improving health.

In the composition of the present invention, in addition to sesamin, gamma -mysterol, and rice germ oil, any other desired component may be blended in a range that does not impair the effect. For example, vitamin E, vitamin C and other vitamins, minerals, hormones, nutrients, spices and other physiologically active ingredients, may also be suitably blended in the formulation of the emulsifier, isotonic agent, buffer , dissolution aids, preservatives, stabilizers, antioxidants, etc.

Since the composition of the present invention has an excellent antioxidant action, it can be used as an antioxidant, and can be used as a composition for exhibiting an antioxidant action in a living body. The use of antioxidants is, for example, associated with elimination of active oxygen in the living body, improvement of liver function, inhibition of the production of lipid peroxides in the body, or improvement of caloric metabolism, and prevention or prevention of diseases or conditions described below; wrinkles of the skin, Spots on the skin, sagging of the skin, fatigue, metabolic syndrome, diabetes, arteriosclerosis, hypertension, cancer, inflammation or aging. Therefore, the antioxidant of the present invention can be effectively applied to such applications.

In vivo absorption enhancer, in vivo absorption promotion method

As described above, by using sesamin, γ -mysterol, and rice germ oil in combination, the in vivo absorption of sesamin can be remarkably enhanced. Therefore, the present invention contains, in another aspect, an in vivo absorption enhancer of sesamin-containing γ -mysterol and rice germ oil as an active ingredient. Further, the present invention relates to a composition for promoting the in vivo absorption of a composition comprising γ -mysterol and rice germ oil as an active ingredient for producing sesamin. Further, the present invention is a method for promoting in vivo absorption of sesamin from the other surface, and is also a method comprising administering the sesamin, gamma -mysterol, and rice germ oil. The subject to be administered has such a need to promote absorption in the body, and includes mammals such as humans.

In the body-absorbing absorbent, composition, and method, the use ratio, blending ratio, blending amount, and intake amount of sesamin, γ -mysterol, and rice germ oil may be combined with sesamin-containing and γ -mysterol. The above values based on the composition of the rice germ oil are appropriately determined based on the above values. The types of sesamin, gamma -mysterol, and rice germ oil used are also the same.

Food and beverages and pharmaceuticals

The composition of the present invention and the absorption promoting agent in the body are suitably supplied to foods and drinks (functional foods, health supplements, nutritional functional foods, special purpose foods, specific health foods, nutritional supplement foods, diet therapy foods, health foods, nutrition). Supplements, etc.) and the form of pharmaceuticals. These foods and drinks and pharmaceuticals include a dietary composition containing sesamin, gamma -mysterol, and rice germ oil, and a pharmaceutical composition, and a food or beverage and a pharmaceutical containing or adding the composition. Further, the present invention also includes a food or drink or a pharmaceutical preparation containing an in vivo absorption agent containing sesamin containing γ -mysterol and rice germ oil as an active ingredient.

In addition, foods and beverages and pharmaceuticals can also be used as pet food, animal feed, and animal foods.

Foods and beverages containing sesamin, gamma -mysterol, and rice germ oil, and pharmaceuticals, are considered to be useful for the purpose of obtaining blood cholesterol lowering action and lowering blood neutral lipid action and improving liver in order to obtain sesamin Functional effects, removal of reactive oxygen species, inhibition of Δ5 desaturase, inhibition of lipid peroxidation, antihypertensive effects, prevention of detoxification, inhibition of breast cancer, and other physiological effects. In addition, foods and beverages and pharmaceuticals for promoting the in vivo absorption of sesamin-containing γ -methanol and rice germ oil can be used for the purpose of causing sesamin to efficiently exhibit various physiological actions as described above.

When the composition of the present invention is provided as a food or drink, the form may be provided in the form of a health food such as a tablet, a capsule, a powder, a granule, a drink (including a solution and a suspension), or a refreshing drink. Milk, beverages, yoghurt and lactic acid bacteria beverages are provided in the form of dairy products, seasonings, processed foods, desserts, snacks (eg chewing gum, candy, jelly), but are not limited to such items.

Further, when the composition of the present invention is used as a pharmaceutical, the administration form may be oral administration, or may be administered in the form of an injection or the like, each of which may be administered. A formulation suitable for administration can be suitably used by a well-known person. For example, a preparation suitable for oral administration includes a tablet, a capsule, a powder, a granule, a solution, a suspension, a syrup, and the like, but is not limited to the dosage forms.

The invention is obtained by separately formulating sesamin, γ -mysterol and rice germ oil, and then taking another preparation at a time equal to or at the same time, after taking one of the preparations, and taking another preparation for the duration of the effect. The desired sesamin-like substances promote absorption in the body. Therefore, a co-formulation of γ -methanol and rice germ oil, a body-inducing agent containing sesamin alone, a reagent group containing a sesamin-based composition, and the like are also included in the scope of the present invention. Each component contained in the reagent group may be ingested at the same time, or may be ingested sequentially or individually.

In the description of the clarification, the numerical range indicated by the upper limit and the lower limit in the present specification, that is, the "lower limit to the upper limit" includes the lower limit and the upper limit. For example, the range indicated by "1~2" includes 1 and 2.

[Examples]

The present invention will be more specifically described by the following examples and examples, but the invention is not limited thereto.

Example 1. Absorption review using the bowel valgus pattern <Experimental method>

SD male rats (7 weeks old) were acclimated in the test environment and used for experiments. Immediately after anesthesia was opened and blood was exsanguin, a small intestine (mainly jejunum) of about 15 cm was taken from the pylorus and divided into two parts. Tied the rope on one side and reversed the inside to the outside, filled with the adjustment solution (internal solution: NaCl 140 mM, CaCl ₂ 0.1 mM, MgCl ₂ 0.5 mM, D-mannose 40 mM, Ascorbic acid 0.8 mM, 10 mM Tris, pH 7. 4) After that, the rope was tied to the other side to make a 5 cm valgus intestine. To 0.5 ml of the control solution or the test solution, 4.5 ml of an adjustment solution containing sodium cholate and albumin was added, and then subjected to ultrasonic treatment for 3 minutes to prepare an emulsified form, which was used as an external solution. The everted intestinal tube was immersed in each external solution, mixed, and then incubated at 37 °C. After 1 hour, the everted intestinal tube was taken out, and the internal liquid was recovered and the weight was measured. 20 μl of the inner solution was taken, diluted 10 times with 50% methanol, centrifuged, and the supernatant was filtered through a filter, and the concentration of the sesamin mixture was measured. The amount of absorption is calculated by multiplying the concentration by the amount of recovered liquid. For the measurement of the mixture of the sesamin, the measurement can be carried out using HPLC using the above-mentioned report (Japanese Patent Laid-Open No. 2009-155312, etc.).

The control group used a mixture of 100 mg of sesamin (sesame: episesamin = 1:1), 250 mg of rice germ oil concentrate (rice germ oil γ 30S: containing 30% γ -mysterol, containing 70% rice germ oil) , Chikuno Food Industry Co., Ltd.), 4250mg of olive oil (Nakaraitesuku Co., Ltd.) heated and mixed evenly, and the test group used 100mg of sesamin mixture (sesame: episesamin = 1:1), 250mg Rice germ oil concentrate (rice germ oil γ 30S: containing 30% γ -mysterol, containing 70% rice germ oil, Chino Food Industry Co., Ltd.), 4250mg rice germ oil (rice germ oil PRO-15: 1.5 % γ -myristyl alcohol, containing 98.5% rice germ oil, Chino Food Industry Co., Ltd.) heated and evenly mixed. That is, the weight ratio of γ -methanol: ( γ -mysterol + rice germ oil) to the composition of the control group was 1:3.3. In the composition of the test group, the weight ratio of γ -methanol: ( γ -mysterol + rice germ oil) was 1:32.4. The control group and the test group were all performed with n=4.

<Result>

The results are shown in Figure 1. The absorption amount of sesamin and episesamin in the inner solution was compared after 1 hour of incubation. The control group was 16.19 μg of sesamin, 16.23 μg of episesamin, 24.29 μg of test group sesamin, and 23.89 μg of episemin. The selenium and the episesamin were also about 1.5 times, and it was confirmed that the absorption amount was remarkably increased. As is clear from the above results, the combined use of sesamin, gamma] - when m furfuryl alcohol and rice germ oil, and further by using a specific ratio gamma] - m rice germ oil and alcohol, can significantly promote the absorption of sesamin-class compounds in vivo. In particular by making γ - m the weight of furfuryl alcohol with respect to γ - m and the ratio of total alcohol of the weight of rice germ oil, compared with the control group the ratio of 1: 3.3 is more reduced, can be obtained in vivo absorption promoting significant effect.

Example 2. Formulation example (Formulation Example 1) granules

After uniformly mixing the above materials, 100 ml of a 10% hydroxypropylcellulose/ethanol solution was added, and mixed, pressed, and dried according to a usual method to obtain granules.

(Formulation Example 2) capsule

The following composition was filled in the capsule skin made of the above ingredients by a usual method to obtain a soft capsule of 360 mg per capsule.

(Formulation Example 3) Beverage agent

The above ingredients were mixed, and water was added to make it 10 liters. The beverage is consumed about 100 ml each time.

(Formulation Example 4) Beverage agent

The above ingredients were mixed, and water was added to make it 10 liters. The beverage is consumed about 100 ml each time.

Example 3. Evaluation of absorption and antioxidant capacity by animal experiment-1 <Experimental method>

Ten SD male rats (7 weeks old) were purchased from Oriental BioService, and one week after acclimation in the experimental environment, they were divided into 5 control groups and 5 experimental groups. After a hunger strike for one night, a mixture of 80 mg of sesamin in the control group (sesamin: episesamin = 1:1), 100 mg of rice germ oil concentrate (rice germ oil γ 30S: containing 30% γ -mysterol, 70% rice germ oil, Chino Food Industry Co., Ltd.), 1700mg of olive oil (Nakaraitesuku Co., Ltd.) heated and mixed evenly, and the test group with 80mg of sesamin mixture (sesame: episesamin = 1: 1), 100mg of rice germ oil concentrate (rice germ oil γ 30S: containing 30% γ -mysterol, containing 70% rice germ oil, Chino Food Industry Co., Ltd.), 1700mg rice germ oil (rice germ oil) PRO-15: 1.5% γ -methanol, containing 98.5% rice germ oil, Chino Food Industry Co., Ltd.), heated and mixed evenly, 2 ml per 1 kg of body weight, using a feeding tube for oral administration. The weight ratio of γ -mysterol: ( γ -mysterol + rice germ oil) was 1:3.3 in the control group and 1:32.4 in the test group. Blood samples were taken from the tail vein using heparin blood collection tubes before administration, and 1, 2, 3, 6, 9, and 24 hours after the start of administration, and plasma samples were obtained by centrifugation (8000 rpm, 10 min). The antioxidant capacity of plasma was measured.

The antioxidant capacity of plasma is determined by ORAC (Oxygen Radical Absorbance Capacity method, oxygen radical absorption capacity) (Reference Radi. Biol. Med. 14 (3), 303-311 (1993), J Agric Food Chem. (10), 4619-4626 (2001), JAgric Food Chem. 50 (7), 1815-1821 (2002), J Agric Food Chem. 53 (10), 4290-4302 (2005), etc.). A high ORAC value indicates a high antioxidant capacity.

<Result>

The difference between the blood ORAC value (μmol TE/L) before administration of each animal and the blood ORAC value (μmol TE/L) of the blood sampling sample after the start of administration was calculated. This value is expressed as the ⊿ORAC value. Figure 2 shows the AUC of the ⊿ORAC value. From Figure 2, it was confirmed that the AUC of the ORAC value of the test group significantly increased compared with the control group. From the above result, by making clear that γ - m the weight of furfuryl alcohol with respect to γ - m and the ratio of total alcohol of the weight of rice germ oil, compared with the control group the ratio of 1: 3.3 is more reduced, enables anti The oxidizing power is significantly increased. Since sesamin is known to have an antioxidant action in the living body, it is considered that the improvement of the antioxidant ability is confirmed to promote the in vivo absorption of sesamin.

Example 4. Evaluation of absorption and antioxidant capacity by animal experiments-2 <Experimental method>

Ten SD male rats (7 weeks old) were purchased from Oriental BioService, and one week after acclimation in the experimental environment, they were divided into 5 control groups and 5 experimental groups. After a hunger strike for one night, a mixture of 80 mg of sesamin in the control group (sesamin: episesamin = 1:1), 1218 mg of rice germ oil (rice germ oil PRO-15: 1.5% gamma -mysterol, containing 98.5 % rice germ oil, Chino Food Industry Co., Ltd.), 3282mg of olive oil (Nakaraitesuku Co., Ltd.) heated and mixed evenly, and the test group was 80mg of sesamin mixture (sesame: episesamin = 1:1) , 60mg rice germ oil concentrate (rice germ oil γ 30S: containing 30% γ -mysterol, containing 70% rice germ oil, Chino Food Industry Co., Ltd.), 4440mg rice germ oil (rice germ oil PRO- 15: 1.5% γ -methanol, containing 98.5% rice germ oil, Chino Food Industry Co., Ltd.), heating and mixing evenly, using 5 ml per 1 kg of body weight, using a feeding tube for oral administration. γ - m furfuryl :( γ - rice + rice germ oil alcohol) weight ratio of 1:67 to control formations, formations in Test 1: 53.2. Before the administration, and 1, 3, 6, 9, and 24 hours after the start of the administration, blood was taken from the tail vein using heparin blood collection tubes, and plasma samples were obtained by centrifugation (8000 rpm, 10 min) to determine plasma. Its antioxidant capacity. The antioxidant capacity of plasma was measured in the same manner as in Example 3.

<Result>

Figure 3 is a graph showing the AUC of the ⊿ORAC value (as defined above). From Figure 3, it was confirmed that the AUC of the ORAC value of the test group significantly increased compared with the control group. From the above result, by making clear that γ - m the weight of furfuryl alcohol with respect to γ - m and the ratio of total alcohol of the weight of rice germ oil, compared with the control group the ratio of 1:67 is more improved anti can The oxidizing power is significantly increased. It is considered that the improvement of the antioxidant ability is confirmed to promote the in vivo absorption of sesamin.

Claims (14)

A composition comprising at least one sesamin, at least one gamma-mysterol, and a rice germ oil, characterized by: a total weight of the gamma-mysterol: (the γ- The total weight of rice sterol and the weight of the germ oil of the rice) = 1 : 4 ~ 1: 66, when the rice germ oil contains γ-mysterol, the weight of the rice germ oil is from the internal γ- The total weight of the rice germ oil of rice sterol removes the weight of the γ-mysterol contained in the rice germ oil, and the sesamin is sesamin and/or episesamin, and the total weight of the aforementioned sesamin is 1 The total weight of the γ-mysterol is 0.3 or more, and the total blending ratio of the γ-mysterol is 0.1 to 50 w/w% based on the total amount of the composition. A composition comprising a liquid dosage form of at least one sesamin, at least one gamma-mysterol, and rice germ oil, characterized by a total weight of the gamma-mysterol : (the total weight of the γ-mysterol and the weight of the rice germ oil) = 1: 4 ~ 1: 66, when the rice germ oil contains γ-mysterol, the weight of the rice germ oil Removing the weight of the γ-mysterol contained in the rice germ oil from the total weight of the rice germ oil containing γ-mysterol, the sesamin is sesamin and/or episesamin, and the sesamin When the total weight is 1, the total weight of the γ-mysterol is 0.3 or more, and the total blending ratio of the γ-mysterol is relative to the The total amount of the composition is 0.0001 to 0.1 w/w%. The composition of claim 1 or 2, wherein the total weight of the γ-mysterol: (the total weight of the aforementioned γ-mysterol and the total weight of the aforementioned rice germ oil) = 1:20 to 1:60. The composition of claim 3, wherein the total weight of the γ-mysterol: (the total weight of the γ-mysterol and the weight of the aforementioned rice germ oil) is 1:25 to 1:55. The composition of claim 4, wherein the total weight of the γ-mysterol: (the total weight of the aforementioned γ-mysterol and the weight of the aforementioned rice germ oil) = 1:32 to 1:49. The composition of claim 1 or 2 is used orally. The composition of claim 1 or 2 is a food or drink. The composition of claim 1 or 2 which is an antioxidant. A use characterized by the use of at least one gamma-mysterol and rice germ oil as an active ingredient for the manufacture of at least one sesamin-promoting in vivo absorbent, the total weight of the aforementioned gamma-mysterol: (previously The total weight of γ-mysterol and the weight of the above-mentioned rice germ oil is 1=4~1:66. When the rice germ oil contains γ-mysterol, the weight of the rice germ oil is from the inside. The total weight of the rice germ oil of γ-mysterol is removed by weight of the weight of γ-mysterol contained in the rice germ oil, and the sesamin is sesamin and/or episesamin, and the total weight of the aforementioned sesamin When it is 1, the total weight of the γ-mysterol is 0.3 or more, and the total blending ratio of the γ-mysterol is relative to the ratio The total amount of the composition is 0.1 to 50 w/w%. Use of a liquid dosage form comprising at least one gamma-mysterol and rice germ oil as an active ingredient for the manufacture of at least one sesamin-promoting agent, the gamma-mysterol Total weight: (the total weight of the aforementioned γ-mysterol and the weight of the aforementioned rice germ oil) = 1:4 to 1:66, when the rice germ oil contains γ-mysterol, the rice germ oil The weight is obtained by removing the weight of the γ-mysterol contained in the rice germ oil from the total weight of the rice germ oil containing γ-mysterol, and the sesamin is sesamin and/or episesamin, and the sesame is obtained. When the total weight of the saccharide is 1, the total weight of the γ-mysterol is 0.3 or more, and the total blending ratio of the γ-mysterol is 0.0001 to 0.1 w/w% based on the total amount of the composition. The use of claim 9 or 10, wherein the total weight of the aforementioned γ-mysterol: (the total weight of the aforementioned γ-mysterol and the weight of the aforementioned rice germ oil) = 1:20 to 1:60. The use of claim 11, wherein the total weight of the γ-mysterol: (the total weight of the aforementioned γ-mysterol and the weight of the aforementioned rice germ oil) = 1:25 to 1:55. The use of claim 12, wherein the total weight of the aforementioned γ-mysterol: (the total weight of the aforementioned γ-mysterol and the weight of the aforementioned rice germ oil) = 1:32 to 1:49. The use of claim 9 or 10, wherein the aforementioned in vivo absorption of the absorbent is oral.