TWI574701B - Small molecular polysaccharide with antioxidation and cosmetics with antioxidation - Google Patents

Description

Small molecule polysaccharide with antioxidant function and makeup with antioxidant function Product

The following description relates to a small molecule polysaccharide having an antioxidant function and a method for producing the same, and in particular, to a polysaccharide extracted from a natural mushroom mites, which is formed by acid hydrolysis or enzymatic hydrolysis to form a smaller molecular mass. Small molecule polysaccharides.

There are many types of antioxidants in human skin, which can be divided into enzyme-type antioxidants and non-enzyme-type antioxidants. The former has Superoxide Dismutase (SOD) and glutathione peroxidation ( Glutathione Peroxidase (GSHP), and hydrogen peroxide (Catalase); the latter have vitamins A, C, E, coenzyme Q10, glutathione, uric acid. Most of the antioxidants are not self-synthesizing, relying on daily intake, and as long as people are alive, every day, a lot of free radicals are generated enough to cause cell damage and death, so we need to be from a variety of foods. Ingest a sufficient amount of antioxidants to maintain normal human function.

On the other hand, the antioxidants that naturally exist in our stratum corneum are mainly vitamins C, E, glutathione and uric acid, which help our skin resist the sun aging that occurs every day. Therefore, vitamin C and E are added to skin care products. In order to meet the demand for antioxidants, in recent years, studies have found that polysaccharides extracted from natural mushroom mites also have antioxidant functions, so they are often regarded as beneficial additives for addition to food or health care products, etc. It can be applied to the formulation of cosmetic raw materials.

However, in general, the extraction methods of common polysaccharides are mostly carried out by liquid fermentation culture of the mycelium of mushroom fungi or directly by using fruit bodies, extraction by hot water extraction or acid-base extraction, and further by organic solvents such as alcohol. The precipitate is purified to obtain a polysaccharide. The molecular weight of the polysaccharide extracted by the conventional method is relatively large, and the human body can not easily absorb it. If it is to be applied to a cosmetic formulation, the cosmetic may not be better resistant to oxidation. The effect.

Therefore, the inventors of the present invention have made improvements in light of the disadvantages of the prior art.

The embodiment of the present invention is directed to a small molecule polysaccharide having an antioxidant function and a method for producing the same, which are extracted from a polysaccharide of a natural mushroom, and then formed by a small molecular mass by acid hydrolysis or enzymatic hydrolysis. The molecular polysaccharide can be easily absorbed by the skin of the human body.

The embodiment of the present invention is directed to a cosmetic having an antioxidant function, and particularly relates to a small molecule polysaccharide extracted from a natural mushroom mites, which has an excellent antioxidant function.

Based on the above object, the present invention provides a method for producing a small molecule polysaccharide having an antioxidant function, which comprises the steps of: obtaining an initial extract by subjecting a mushroom to an extraction. The initial extract is precipitated into the crude polysaccharide by a precipitation procedure. And adding an acidic solvent or a polysaccharide hydrolyzing enzyme to the crude polysaccharide to form a mixture, and subjecting the mixture to a hydrolysis procedure and a freeze-drying procedure to obtain a small molecule polysaccharide. The molecular mass of the small molecule polysaccharide is between 3k and 30k Dalton (Da) and the small molecule polysaccharide is ferrous ion (Fe ²⁺ ) when the wavelength of the light in the absorption spectrum is 562nm. The half inhibitory concentration (IC ₅₀ ) of the activity was 1443 ppm.

Preferably, the mushroom genus may comprise a ginseng.

Preferably, the acidic solvent comprises trifluoroacetic acid, sulfuric acid or hydrochloric acid.

Preferably, the polysaccharide hydrolyzing enzyme comprises β-1,3-D-glucanase (β-1,3-D-glucanase).

Preferably, the small molecule polysaccharide has a half-inhibitory concentration (IC ₅₀ ) of ABTS cationic radical (ABTS ^.+ ) activity of 182 ppm by a second absorption spectroscopy experiment, and the second absorption spectrum experimental light wavelength is 734 nm. .

Based on the above purposes, the present invention further provides an anti-oxidation cosmetic, wherein the anti-oxidation cosmetic comprises a small molecule polysaccharide, and the molecular mass of the small molecule polysaccharide is between 3k and 30k Dalton (Daton). The half-inhibitory concentration (IC ₅₀ ) of the ferrous ion (Fe ²⁺ ) activity was 1,443 ppm when the wavelength of the small molecule polysaccharide was 562 nm in an absorption spectroscopy experiment. And a carrier for delivering a small molecule polysaccharide.

Preferably, the small molecule polysaccharide system is extracted from the mushroom mites.

Preferably, the mushroom genus may comprise a ginseng.

Based on the above purposes, the present invention further provides a small molecule polysaccharide which is not extracted via mushroom mites, wherein the molecular mass of the small molecule polysaccharide is between 3k and 30k Dalton (Da).

Preferably, the mushroom genus may comprise a ginseng.

10‧‧‧Small molecule polysaccharides

20‧‧‧Antioxidant cosmetics

210‧‧‧ Carrier

S1~S3‧‧‧ steps

The above-mentioned and other features and advantages of the present invention will become more apparent from the detailed description of the exemplary embodiments of the accompanying drawings in which: FIG. 1 is a small molecule polysaccharide having an antioxidant function according to the present invention. Flow chart of the manufacturing method of the body.

Fig. 2 is a graph showing the analysis of the ability of the ferrous ion of the small molecule polysaccharide having an antioxidant function according to the present invention.

Fig. 3 is an experimental test chart showing the total antioxidant capacity of the small molecule polysaccharide having an antioxidant function according to the present invention.

Fig. 4 is a graph showing the analysis of the reducing power of the small molecule polysaccharide having an antioxidant function according to the present invention.

Figure 5 is a block diagram of a cosmetic having an antioxidant function according to the present invention.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items. When the phrase "at least one of" is preceded by a list of elements, the entire list of elements is modified instead of the individual elements in the list.

Please refer to Fig. 1, which is a flow chart of a method for producing a small molecule polysaccharide having an antioxidant function according to the present invention. As shown in Fig. 1, step S1 obtains an initial extract by subjecting a mushroom to an extraction. Step S2 uses a precipitation procedure to precipitate the primary extract into the crude polysaccharide. Step S3 adds an acidic solvent or a polysaccharide hydrolyzing enzyme to the crude polysaccharide to form a mixture, and the mixture is subjected to a hydrolysis procedure and a freeze-drying procedure to obtain a small molecule polysaccharide.

In detail, the mushroom genus may comprise a black ginseng, the precipitation procedure is performed by using ethanol for precipitation, the acidic solvent comprises trifluoroacetic acid, sulfuric acid or hydrochloric acid, and the polysaccharide hydrolyzing enzyme may comprise a β-1,3-D - glucanase (β-1,3-D-glucanase), and the molecular mass of the obtained small molecule polysaccharide 10 is between 3k and 30k Dalton (Daton), and the small molecule polysaccharide 10 The half-inhibitory concentration (IC ₅₀ ) of ferrous ion (Fe ²⁺ ) activity in an absorption spectroscopy experiment at a wavelength of 562 nm was 1443 ppm.

Please refer to FIG. 2, which is a small molecule polysaccharide having antioxidant function according to the present invention. Analytical test chart of the ability of the body to combine ferrous ions. As shown in the ferrous ion capacity test of Figure 2, the x-axis represents the concentration of a part of the polysaccharide of the ginseng ginseng and the small molecule polysaccharide 10 manufactured by the genus ginseng in the present invention. The y-axis represents the percentage of ferrous iron ions. The molecular weight of the polysaccharides of the ginseng ginseng is between 500k and 1000k Daltons (Dalton, Da), while the molecular mass of the small molecule polysaccharides 10 is between 3,000 and 30,000. Between Dalton (Da), the wavelength of the light in the absorption spectroscopy experiment was set to 562 nm.

The prooxidation of metal ions is often the main cause of lipid oxidation. By redox reaction, only a small amount of metal ions can generate free radicals and accelerate lipid oxidation, and ferrous ions (Fe ²⁺ ) are It has the most pro-oxidative action to promote the progress of lipid oxidation. In this experiment, the semi-inhibitory concentration (IC ₅₀ ) of lintel ion (Fe ²⁺ ) activity of Wulingshen polysaccharide was 2586 ppm, while the small molecule polysaccharide of Wuling ginseng 10 was ferrous ion (Fe ²⁺ ) active. The half-inhibitory concentration (IC ₅₀ ) was 1,443 ppm, and it was found that the small molecule polysaccharide 10 of the mulberry ginseng had a preferable oxidation inhibiting effect.

Please refer to Fig. 3, which is an analytical test chart of the total oxidizing power of the small molecule polysaccharide having an antioxidant function according to the present invention. As shown in the total antioxidant capacity test of Fig. 3, the x-axis represents the concentration of the polysaccharide of the ginseng ginseng and the small molecule polysaccharide 10 produced from the ginseng ginseng in the present invention in the solution, y The axis represents the percentage of total antioxidant capacity, and the molecular weight of the polysaccharides of Wulingshen is between 500k and 1000k Daltons (Dalton, Da), while the molecular mass of small molecule polysaccharides 10 is between 3,000 and 30,000 Daltons. Between (Dalton, Da), and the wavelength of the light in this absorption spectroscopy experiment was 734 nm.

In this test, ABTS undergoes an oxidation reaction with hydrogen peroxide and a peroxidase to form a blue-green water-soluble ABTS cationic radical (ABTS ^.+ ). When the ABTS cationic radical (ABTS ^.+ ) is reduced with an antioxidant, The color of the solution will be lighter and the absorbance will decrease or disappear. Therefore, the lower the absorbance value, the stronger the ability of the sample to clear the ABTS cationic radical (ABTS ^.+ ), and then evaluate the ability of the sample to provide hydrogen proton scavenging free radicals. .

In this test, the semi-inhibitory concentration (IC ₅₀ ) of the polysaccharide of the ginseng ginseng on the ABTS cation activity was 848 ppm, and the semi-inhibitory concentration of the small molecule polysaccharide 10 of the ginseng ginseng on the ABTS cation free radical (ABTS ^.+ ) activity. (IC ₅₀ ) was 182 ppm, and it was found that the small molecule polysaccharide 10 of the mulberry ginseng had an effect of preferably suppressing oxidation.

Please refer to Fig. 4, which is an analytical test chart of the reducing power of the small molecule polysaccharide having an antioxidant function according to the present invention. As shown in the reducing force test of Fig. 4, the x-axis represents the concentration of the polysaccharide of the ginseng ginseng and the small molecule polysaccharide 10 produced from the ginseng ginseng in the present invention in the solution, and the y-axis is The production of Prussian blue is used as an indicator to detect the magnitude of the reducing force by the change in absorbance at 700 nm. If the absorbance is higher, the reduction rate is higher. The molecular weight of the polysaccharides of Wulingshen is between 500k and 1000k Daltons (Dalton, Da), while the molecular mass of small molecule polysaccharides 10 is between 3k~30k Daltons (Daton). In this test, the reducing power of both increased with the increase of concentration, but when the concentration of the polysaccharide and small molecule polysaccharide 10 was between 4000ppm and 5000ppm, the absorbance of the former was about 0.2~0.3. Between the small molecular polysaccharides 10 and the light absorption value of about 0.9 to 1.1, it can be seen that the small molecule polysaccharide 10 produced by the present invention has a reducing power of about 3~ 4 times.

Please refer to Fig. 5, which is a block diagram of a cosmetic 20 having an antioxidant function according to the present invention. As shown in Fig. 5, the anti-oxidation cosmetic 20 comprises a small molecule polysaccharide 10, wherein the molecular mass of the small molecule polysaccharide 10 is between 3k and 30k Dalton (Daton) and the small molecule polysaccharide The bulk 10 has a half-inhibitory concentration (IC ₅₀ ) of ferrous ion (Fe ²⁺ ) activity of 1443 ppm in an absorption spectrum experiment at a wavelength of 562 nm, and a carrier 210 for transporting the small molecule polysaccharide 10. The small molecule polysaccharide 10 is extracted from a mushroom, and the mushroom can contain a black ginseng.

In summary, it can be seen that the polysaccharide extracted from the natural mushroom mites and the small molecular polysaccharide having a small molecular mass formed by the hydrolysis of the enzyme can be easily absorbed by the skin of the human body, and it is known through different experiments. Molecular polysaccharides have excellent anti-oxidation ability and are extremely suitable as cosmetics for use on the skin.

Although the invention has been particularly shown and described with reference to the exemplary embodiments thereof, It will be apparent to those skilled in the art that various changes in form and detail may be made without departing from the spirit and scope of the invention.

S1~S3‧‧‧ steps

Claims (2)

The anti-oxidation cosmetic comprises: a small molecule polysaccharide, wherein the molecular mass of the small molecule polysaccharide is between 3k and 30k Dalton (Da) and the The small molecule polysaccharide has a half-inhibitory concentration (IC50) of ferrous ion (Fe ²⁺ ) activity of 1443 ppm at an optical wavelength of 562 nm in an absorption spectroscopy experiment; and a carrier for transferring the small molecule polysaccharide, wherein The small molecule polysaccharide system is obtained by hydrolyzing a polysaccharide of the Wulingshen fruit body with a β-1,3-D-glucanase. A small molecule polysaccharide is obtained by extracting a mushroom polysaccharide, wherein the molecular mass of the small molecule polysaccharide is between 3k and 30k Dalton (Da), and the source of the mushroom is The lingko ginseng fruit body, wherein the small molecule polysaccharide system Wuling ginseng fruit body polysaccharide is obtained by hydrolysis of a β-1,3-D-glucanase.