TWI542684B - Cell culture apparatus and method of using the same - Google Patents
Cell culture apparatus and method of using the same Download PDFInfo
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Description
本發明係涉及一種細胞培養裝置，特別是指可自動化移動培養盒於作業區。本發明另涉及一種使用方法，特別是指執行前述之細胞培養裝置的方法。The present invention relates to a cell culture device, and more particularly to an automated mobile culture cartridge in a work area. The invention further relates to a method of use, in particular to a method of performing the aforementioned cell culture apparatus.
近年來，細胞培養的技術已經發展地相當成熟，所培養的細胞已經被應用在各種不同的領域，以至於培養細胞目前正急需要被大量製造。由於細胞培養牽涉多個步驟，因此需雇請多名技術人員來移動裝有細胞培養液的培養盒，以利在不同作業區之間執行不同的步驟。然而，雇請多名員工將造成成本的提高且仍然缺乏效率。In recent years, the technology of cell culture has been developed to be quite mature, and the cultured cells have been applied in various fields, so that cultured cells are currently in urgent need of being mass-produced. Because cell culture involves multiple steps, multiple technicians are hired to move the culture cartridge containing the cell culture fluid to facilitate different steps between different work areas. However, hiring multiple employees will result in increased costs and still lack efficiency.
現有技術中，使用機器手臂模仿人為培養細胞步驟的自動化細胞培養裝置，成為目前主要發展方向。例如美國專利公告第8,883,492號揭示一種節省空間的細胞培養裝置，使設備之間達成最小距離，該細胞培養裝置包含一細胞培養室、一冷凍機、一控制單元以及一中間室。In the prior art, an automated cell culture device that uses a robotic arm to mimic the steps of artificially culturing cells has become the main development direction. For example, U.S. Patent No. 8,883,492 discloses a space-saving cell culture apparatus that provides a minimum distance between devices, a cell culture chamber, a freezer, a control unit, and an intermediate chamber.
然而，當細胞於培養階段時該細胞培養裝置的其他設備將會被閒置無用，且一旦任何空間或設備遭受到汙染時，整個細胞培養裝置將需要被關閉且培養中的細胞則必須進行滅菌與拋棄。有鑑於此，現有技術之細胞培養裝置，仍有待改善無法有效利用設備與汙染難以清理的缺陷。However, when the cells are in the culture phase, the other equipment of the cell culture device will be left unused, and once any space or device is contaminated, the entire cell culture device will need to be shut down and the cells in the culture must be sterilized and abandon. In view of this, the prior art cell culture device still has to be improved in the inability to effectively utilize the equipment and the defects that the pollution is difficult to clean.
為了克服現有技術中細胞培養裝置的缺陷，本發明係關於一種細胞培養裝置，尤其是該細胞培養裝置係為自動化移動培養盒於作業區，以利執行各種不同作業。In order to overcome the deficiencies of the cell culture apparatus of the prior art, the present invention relates to a cell culture apparatus, and in particular, the cell culture apparatus is an automated mobile culture box in a work area for performing various operations.
為達到上述之發明目的，本發明所採用的技術手段為提供一種細胞培養裝置，其包含至少一儲存箱體、至少一屏障以及一作業箱體。其中該至少一儲存箱體包含一培養架、一直立軌道以及一旋轉式夾具；該培養架係設於該至少一儲存箱體；該直立軌道係與該培養架相鄰；該旋轉式夾具，其係與該直立軌道可移動地相連接。該至少一屏障係相鄰於該至少一儲存箱體之直立軌道，且至少一屏障包含一第一閘門。該作業箱體具有相對的兩面，其包含一填充區以及一觀察區；該填充區包含一第一輸送裝置、至少一開關裝置以及至少一填充裝置；該第一輸送裝置具有相對的兩側，該第一輸送裝置可移動地固設於該作業箱體，且該第一輸送裝置的一側係相鄰於該至少一屏障；該至少一開關裝置係固設於該作業箱體，且該至少一開關裝置係相鄰於該第一輸送裝置的另一側；該至少一填充裝置係相鄰於該至少一開關裝置與該第一輸送裝置。該觀察區包含至少一第一觀察裝置以及一第二輸送裝置；其中該至少一第一觀察裝置係固設於該觀察區，且該至少一第一觀察裝置係相鄰於該填充區之至少一開關裝置；該第二輸送裝置係與該至少一第一觀察裝置相連接。In order to achieve the above object, the technical means adopted by the present invention is to provide a cell culture device comprising at least one storage case, at least one barrier and a work box. The at least one storage box comprises a culture frame, an upright track and a rotary clamp; the culture frame is disposed on the at least one storage case; the upright track is adjacent to the culture frame; the rotary clamp, It is movably connected to the upright track. The at least one barrier is adjacent to the upright track of the at least one storage case, and the at least one barrier comprises a first gate. The working box has two opposite sides, and includes a filling area and an observation area; the filling area comprises a first conveying device, at least one switching device and at least one filling device; the first conveying device has opposite sides, The first conveying device is movably fixed to the working box, and one side of the first conveying device is adjacent to the at least one barrier; the at least one switching device is fixed to the working box, and the At least one switching device is adjacent to the other side of the first conveying device; the at least one filling device is adjacent to the at least one switching device and the first conveying device. The observation area includes at least one first observation device and a second delivery device; wherein the at least one first observation device is fixed in the observation area, and the at least one first observation device is adjacent to the filling area a switching device; the second conveying device is coupled to the at least one first viewing device.
較佳的，所述之至少一儲存箱體更進一步包含一固設於該至少一儲存箱體的燈管。Preferably, the at least one storage box further comprises a lamp tube fixed to the at least one storage box.
較佳的，所述之作業箱體更進一步包含一固設於該填充區一側的收集區。Preferably, the working box further comprises a collecting area fixed on one side of the filling area.
較佳的，所述之第二輸送裝置為一機器手臂。Preferably, the second conveying device is a robot arm.
較佳的，所述之觀察區更進一步包含至少一第二觀察裝置，該至少一第二觀察裝置係位於該至少一第一觀察裝置與該第一輸送裝置之間。Preferably, the observation area further comprises at least one second observation device, the at least one second observation device being located between the at least one first observation device and the first delivery device.
較佳的，所述之細胞培養裝置更進一步包含一滅菌箱體以及一資訊處理設備；該滅菌箱體係與該作業箱體的一側相連接，且該作業箱體的另一側係與該至少一屏障相連接；該資訊處理設備係相鄰於該作業箱體。Preferably, the cell culture device further comprises a sterilization box and an information processing device; the sterilization box system is connected to one side of the working box, and the other side of the working box is coupled to the At least one barrier is connected; the information processing device is adjacent to the work box.
更佳的，所述之滅菌箱體包含一第二閘門以及一紫外線滅菌裝置；該第二閘門係相鄰於該作業箱體；該紫外線滅菌裝置係固設於該滅菌箱體。More preferably, the sterilization box comprises a second gate and an ultraviolet sterilization device; the second gate is adjacent to the working box; the ultraviolet sterilization device is fixed to the sterilization box.
更佳的，所述之滅菌箱體為一無菌操作台。More preferably, the sterilization chamber is an aseptic workstation.
較佳的，所述之至少一儲存箱體為三個彼此相鄰的儲存箱體。Preferably, the at least one storage box is three storage boxes adjacent to each other.
本發明又提供一種如上所述之細胞培養裝置的方法，其中該使用細胞培養裝置的方法包含以下步驟：裝載複數培養盒於該至少一儲存箱體的培養架；以該至少一儲存箱體的旋轉式夾具夾取其中一培養盒；開啟該至少一屏障之第一閘門，夾有該培養盒的該旋轉式夾具旋轉後輸送經過該第一閘門至該作業箱體之填充區之第一輸送裝置；在第一輸送裝置之培養盒歷經以下至少一流程：一填充流程、一觀察流程以及一收集流程；以該旋轉式夾具從該第一輸送裝置夾取該進行過至少一流程後的培養盒並輸送回該培養架進行培養。The present invention further provides a method of the cell culture apparatus as described above, wherein the method of using the cell culture apparatus comprises the steps of: loading a plurality of culture cartridges in the culture rack of the at least one storage tank; and the at least one storage tank The rotary clamp clamps one of the culture boxes; the first gate of the at least one barrier is opened, and the rotary clamp of the culture cartridge is rotated and then conveyed through the first gate to the first delivery of the filling area of the working box a device; the culture box of the first conveying device undergoes at least one of the following processes: a filling process, an observation process, and a collecting process; and the rotating jig is used to clamp the culture after the at least one process from the first conveying device The cassette is transported back to the culture frame for cultivation.
較佳的，所述之方法更進一步包含當該至少一儲存箱體正在培養細胞時，替換或增加複數儲存箱體於該細胞培養裝置，且複數儲存箱體係彼此相鄰。Preferably, the method further comprises replacing or adding a plurality of storage tanks to the cell culture device when the at least one storage tank is culturing the cells, and the plurality of storage tank systems are adjacent to each other.
較佳的，所述之填充流程之步驟係藉由該至少一開關裝置將置於該第一輸送裝置之培養盒的蓋子開啟；以該至少一填充裝置將該培養盒進行填充；以該至少一開關裝置將該培養盒的蓋子蓋上。Preferably, the step of filling the process is to open a cover of the culture box placed in the first conveying device by the at least one switch device; filling the culture box with the at least one filling device; A switch device covers the lid of the incubator.
較佳的，所述之觀察流程之步驟係藉由該觀察區的第二輸送裝置將置於第一輸送裝之培養盒輸送至該至少一第一觀察裝置，再以該第二輸送裝置將該培養盒輸送至該第一輸送裝置。Preferably, the step of the observation process is to transport the culture box placed in the first transport device to the at least one first observation device by the second transport device of the observation area, and then the second transport device The incubator is delivered to the first delivery device.
更佳的，所述之觀察流程之步驟更進一步包含藉由觀察區的第二輸送裝置將置於第一輸送裝置之培養盒先輸送至該至少一第二觀察裝置後，再將位於該至少一第二觀察裝置的培養盒輸送至該至少一第一觀察裝置，最後將該培養盒輸送至該第一輸送裝置。More preferably, the step of the observation process further comprises: after the culture box placed in the first transport device is first transported to the at least one second observation device by the second transport device of the observation zone, and then located at least A culture cartridge of a second observation device is delivered to the at least one first observation device, and finally the culture cartridge is delivered to the first delivery device.
較佳的，所述之收集流程之步驟係藉由該至少一開關裝置將置於第一輸送裝置之培養盒的蓋子開啟；以該第一輸送裝置將該培養盒軸向旋轉而使置於該培養盒中的液體被傾倒至收集區；以該至少一填充裝置該培養盒進行填充；以該至少一開關裝置將該培養盒的蓋子蓋上。Preferably, the step of the collecting process is to open the cover of the culture box placed in the first conveying device by the at least one switching device; and the first conveying device axially rotates the culture box to be placed The liquid in the incubator is poured into a collection zone; the incubator is filled with the at least one filling device; the lid of the incubator is capped with the at least one switching device.
本發明的優點在於：The advantages of the invention are:
1. 本發明之細胞培養裝置可藉由可自動地移動該培養盒，並執行各種不同流程，以達成節省人力的功效。1. The cell culture apparatus of the present invention can achieve labor saving effects by automatically moving the incubator and performing various processes.
2. 當培養盒中的細胞正在進行細胞培養時，藉由將該進行培養細胞中的儲存箱體被置換成另一儲存箱體，或擴增其他儲存箱體，以達成使作業箱體可持續運作而不會閒置。2. When the cells in the culture cassette are undergoing cell culture, by replacing the storage case in the cultured cells with another storage case, or amplifying other storage cases, the work box can be made Continue to operate without being idle.
3. 藉由該屏障區隔該儲存箱體與該作業箱體，以達成減少汙染的效果。3. The barrier is used to separate the storage box from the work box to achieve the effect of reducing pollution.
4. 藉由該滅菌箱體與該作業箱體的結合，使得位於作業箱體中的拋棄式試管或遭汙染的裝置，可被快速的輸送置滅菌箱體進行消毒滅菌。4. By the combination of the sterilization box and the working box, the disposable test tube or the contaminated device located in the working box can be quickly sterilized and sterilized by the delivery sterilization box.
以下配合圖式及本發明之較佳實施例，進一步闡述本發明為達成目的所採取的技術手段。The technical means adopted by the present invention for achieving the object are further explained below in conjunction with the drawings and the preferred embodiments of the present invention.
請參閱圖1及圖2所示，本發明提供一種細胞培養裝置，其包含一儲存箱體10、一屏障20、一作業箱體30、一滅菌箱體40以及一資訊處理設備50。Referring to FIG. 1 and FIG. 2, the present invention provides a cell culture device comprising a storage box 10, a barrier 20, a working box 30, a sterilization box 40, and an information processing device 50.
請參閱圖1至圖3所示，該儲存箱體10包含一培養架11、一直立軌道12、一旋轉式夾具13以及一燈管14。該培養架11係固設於該儲存箱體10；該直立軌道12係相鄰於該培養架11；該旋轉式夾具13係與該直立軌道12可轉動地與可移動地相連接；該燈管14係固設於該儲存箱體10；在一較佳的實施例中，該燈管14為一發光二極體(light-emitting diode，LED)燈管或一紫外線燈管。當該儲存箱體10正在培養細胞時，置換成另一儲存箱體10於該細胞培養裝置，該儲存箱體10為可置換式且使得該作業箱體30可以持續進行作業而非閒置。Referring to FIGS. 1 to 3 , the storage case 10 includes a culture frame 11 , an upright track 12 , a rotary clamp 13 , and a light tube 14 . The culture frame 11 is fixed to the storage box 10; the vertical rail 12 is adjacent to the culture frame 11; the rotary clamp 13 is rotatably and movably connected to the vertical rail 12; The tube 14 is fixed to the storage box 10; in a preferred embodiment, the tube 14 is a light-emitting diode (LED) tube or an ultraviolet tube. When the storage case 10 is culturing the cells, it is replaced with another storage case 10 in the cell culture apparatus, and the storage case 10 is replaceable and allows the work case 30 to continue to operate rather than idle.
該屏障20係相鄰於該儲存箱體10之直立軌道12，且該屏障20係位於該儲存箱體10與該作業箱體30之間。該屏障20包含一第一閘門21，該第一閘門21可允許該旋轉式夾具13通過並進入作業箱體30。The barrier 20 is adjacent to the upright track 12 of the storage case 10, and the barrier 20 is located between the storage case 10 and the work case 30. The barrier 20 includes a first gate 21 that allows the rotary clamp 13 to pass through and into the work compartment 30.
請參閱圖3至圖5所示，該作業箱體30具有相對的兩面，該作業箱體30包含一填充區31、一觀察區32以及一收集區33。該填充區31包含一第一輸送裝置311、至少一開關裝置312以及至少一填充裝置313。該第一輸送裝置311具有相對的兩側，該第一輸送裝置311可移動地固設於該作業箱體30，且該第一輸送裝置311的一側係相鄰於該屏障20；該至少一開關裝置312係固設於該作業箱體30，且該至少一開關裝置312係相鄰於該第一輸送裝置311的另一側；該至少一填充裝置313係相鄰於該至少一開關裝置312與該第一輸送裝置311。請參閱圖5及圖6所示，該觀察區32包含至少一第一觀察裝置321、一第二輸送裝置322以及至少一第二觀察裝置323。該至少一第一觀察裝置321係固設於該觀察區32，且該至少一第一觀察裝置321係相鄰於該填充區31之至少一開關裝置312。在一較佳的實施例中，該至少一第一觀察裝置321是一顯微鏡，可用以觀察細胞是否有受到汙染。在另一較佳的實施例中，該至少一第一觀察裝置321藉由與已建構在資料庫中正常的細胞形態進行比對，判斷偵測到的細胞形態是否正常。該第二輸送裝置322係固設於該至少一第一觀察裝置321，且該第二輸送裝置322可來回輸送一培養盒於該第一輸送裝置311與該至少一第一觀察裝置321之間。又一較佳的實施例中，該第二輸送裝置322為一機器手臂。該至少一第二觀察裝置323係位於該至少一第一觀察裝置321與該第一輸送裝置311之間。在一較佳的實施例中，該至少一第二觀察裝置323為一具相機功能的細胞觀察裝置，該細胞觀察裝置可以二倍至十倍的放大倍率下觀察細胞狀況。請參閱圖4所示，該收集區33係固設於該填充區31的一側。在一較佳的實施例中，該收集區33係固設於該填充區31的下方。該作業箱體30藉由該屏障20而呈密封狀態，因此當要擴增多個儲存箱體10、或是要置換成另一個儲存箱體10時，該作業箱體30可保持無菌狀態。Referring to FIGS. 3 to 5 , the working box 30 has opposite sides. The working box 30 includes a filling area 31 , an observation area 32 , and a collecting area 33 . The filling area 31 includes a first conveying device 311, at least one switching device 312, and at least one filling device 313. The first conveying device 311 has opposite sides, the first conveying device 311 is movably fixed to the working box 30, and one side of the first conveying device 311 is adjacent to the barrier 20; A switching device 312 is fixed to the working box 30, and the at least one switching device 312 is adjacent to the other side of the first conveying device 311; the at least one filling device 313 is adjacent to the at least one switch Device 312 and the first delivery device 311. Referring to FIG. 5 and FIG. 6 , the observation area 32 includes at least one first observation device 321 , a second delivery device 322 , and at least one second observation device 323 . The at least one first viewing device 321 is fixed to the viewing area 32, and the at least one first viewing device 321 is adjacent to at least one switching device 312 of the filling area 31. In a preferred embodiment, the at least one first viewing device 321 is a microscope that can be used to observe whether the cells are contaminated. In another preferred embodiment, the at least one first viewing device 321 determines whether the detected cell morphology is normal by comparing with normal cell morphology that has been constructed in the database. The second conveying device 322 is fixed to the at least one first viewing device 321 , and the second conveying device 322 can transport a culture box between the first conveying device 311 and the at least one first viewing device 321 . . In another preferred embodiment, the second delivery device 322 is a robotic arm. The at least one second viewing device 323 is located between the at least one first viewing device 321 and the first conveying device 311. In a preferred embodiment, the at least one second viewing device 323 is a camera-operated cell viewing device that can observe cell conditions at a magnification of two to ten times. Referring to FIG. 4, the collection area 33 is fixed to one side of the filling area 31. In a preferred embodiment, the collection area 33 is fixed below the filling area 31. The work box 30 is sealed by the barrier 20, so that when a plurality of storage cases 10 are to be expanded or replaced with another storage case 10, the work case 30 can remain sterile.
請參閱圖1及圖2所示，該滅菌箱體40係與該作業箱體30的一面相結合，且該作業箱體30的另一面係與該屏障20相結合。該滅菌箱體40包含一第二閘門41以及一紫外線滅菌裝置42。該第二閘門41係相鄰於該作業箱體30之填充區32；該紫外線滅菌裝置42係固設於該滅菌箱體40。在一較佳的實施例中，該滅菌箱體40為一無菌操作台。於作業箱體30內的設備可經由該第二閘門41輸送至該滅菌箱體40。Referring to FIGS. 1 and 2, the sterilization chamber 40 is coupled to one side of the work box 30, and the other side of the work box 30 is coupled to the barrier 20. The sterilization chamber 40 includes a second gate 41 and an ultraviolet sterilization device 42. The second shutter 41 is adjacent to the filling area 32 of the working box 30; the ultraviolet sterilizing device 42 is fixed to the sterilization box 40. In a preferred embodiment, the sterilization chamber 40 is an aseptic workstation. The equipment in the work box 30 can be transported to the sterilization chamber 40 via the second gate 41.
該資訊處理設備50係相鄰於該儲存箱體10與該作業箱體30。該資訊處理設備50包括一電腦，該電腦做為分析數據與接受來自該觀察區32傳來的影像，並可控制位於該儲存箱體10與該作業箱體30內的裝置以及該屏障20的第一閘門21。The information processing device 50 is adjacent to the storage case 10 and the work box 30. The information processing device 50 includes a computer that analyzes data and receives images from the viewing area 32, and controls devices located in the storage case 10 and the work box 30 and the barrier 20 The first gate 21.
請參閱圖7所示，本發明提供另一種細胞培養裝置，其包含三儲存箱體10A、三屏障20A、一作業箱體30A、一滅菌箱體40A以及一資訊處理設備50A。該等儲存箱體10A彼此依序相鄰，該等屏障20A具有相對的兩面，各儲存箱體10A分別與各屏障20A的一面相結合，各屏障20A的另一面與該作業箱體30A相結合。該作業箱體30A具有相對的兩面，該作業箱體30A的其中一面係與該滅菌箱體40A與該資訊處理設備50A相結合，且該滅菌箱體40A係相鄰於該資訊處理設備50A，該作業箱體30A的另一面係與該等屏障20A相結合。Referring to FIG. 7, the present invention provides another cell culture device comprising three storage tanks 10A, three barriers 20A, a working cabinet 30A, a sterilization chamber 40A, and an information processing device 50A. The storage tanks 10A are sequentially adjacent to each other, and the barriers 20A have opposite sides, each storage box 10A is combined with one side of each barrier 20A, and the other side of each barrier 20A is combined with the working box 30A. . The working box body 30A has opposite sides, one side of the working box body 30A is combined with the sterilization box body 40A and the information processing apparatus 50A, and the sterilization box body 40A is adjacent to the information processing apparatus 50A. The other side of the work box 30A is combined with the barriers 20A.
本發明又提供一種如上所述之細胞培養裝置的方法，其中該使用細胞培養裝置的方法包含以下步驟：裝載複數培養盒於該至少一儲存箱體10、10A的培養架11；以該至少一儲存箱體10、10A的旋轉式夾具13夾取其中一培養盒；開啟至少一屏障20、20A之第一閘門21，夾有該培養盒的該旋轉式夾具13旋轉後輸送經過該第一閘門21至該作業箱體30、30A之填充區31之第一輸送裝置311；使該培養盒歷經以下至少一流程：一填充流程、一觀察流程以及一收集流程；以該旋轉式夾具13從該第一輸送裝置311夾取該歷經至少一流程後的培養盒並輸送回該至少一儲存箱體10、10A之培養架11進行培養。The present invention further provides a method of the cell culture apparatus as described above, wherein the method of using the cell culture apparatus comprises the steps of: loading a plurality of culture cassettes in the culture rack 11 of the at least one storage tank 10, 10A; The rotary clamp 13 of the storage case 10, 10A grips one of the culture boxes; opens the first gate 21 of at least one of the barriers 20, 20A, and the rotary clamp 13 sandwiching the culture cartridge is rotated and transported through the first gate 21 to the first conveying device 311 of the filling area 31 of the working box 30, 30A; the culture box is subjected to at least one of the following processes: a filling process, an observation process, and a collecting process; The first conveying device 311 grips the culture cartridge that has passed through at least one flow and transports it back to the culture rack 11 of the at least one storage tank 10, 10A for cultivation.
請參閱圖3至圖5及圖8所示，其中填充流程主要於該作業箱體30、30A的填充區31完成，其步驟包括，藉由該至少一開關裝置312將置於該第一輸送裝置311之該培養盒的蓋子開啟(S101)；該至少一填充裝置313將該培養盒進行填充(S102)；該至少一開關裝置312將該培養盒的蓋子蓋上(S103)。Referring to FIG. 3 to FIG. 5 and FIG. 8 , the filling process is mainly performed on the filling area 31 of the working box 30 , 30A , and the step includes: placing the first conveying by the at least one switching device 312 . The lid of the incubator of the device 311 is opened (S101); the at least one filling device 313 fills the incubator (S102); the at least one switch device 312 covers the lid of the incubator (S103).
請參閱圖4至圖6及圖9所示，其中觀察流程主要於該作業箱體30、30A的觀察區32完成，其步驟包括，藉由該觀察區32的第二輸送裝置322將該置於第一輸送裝置311之培養盒輸送至該至少一第二觀察裝置323(S201)。以該至少一第二觀察裝置323觀察該培養盒內部，以確認培養於該培養盒內的細胞狀態是否正常。接著，以該觀察區32的第二輸送裝置322將位於該至少一第二觀察裝置323的培養盒輸送至該至少一第一觀察裝置321(S202)，藉由該至少一第一觀察裝置321觀察該培養盒內部，並進行細胞計數並確認細胞形態是否正常。觀察完畢後，該第二輸送裝置322將位於該至少一第一觀察裝置321的培養盒輸送回該第一輸送裝置311(S203)。Please refer to FIG. 4 to FIG. 6 and FIG. 9 , wherein the observation process is mainly performed on the observation area 32 of the working box 30 , 30A , and the step includes the second conveying device 322 of the observation area 32 . The culture cartridge of the first transport device 311 is transported to the at least one second observation device 323 (S201). The inside of the incubator is observed by the at least one second observation device 323 to confirm whether the state of the cells cultured in the incubator is normal. Next, the culture box located in the at least one second observation device 323 is transported to the at least one first observation device 321 by the second transport device 322 of the observation area 32 (S202), by the at least one first observation device 321 The inside of the incubator was observed, and cell count was performed to confirm whether the cell morphology was normal. After the observation, the second conveying device 322 conveys the culture box located in the at least one first observation device 321 back to the first conveying device 311 (S203).
請參閱圖3至圖5及圖10所示，其中收集流程主要於該作業箱體30、30A的收集區33完成，其步驟包括，藉由該至少一開關裝置312將置於該第一輸送裝置311的培養盒的蓋子開啟(S301)。該第一輸送裝置311將該培養盒軸向旋轉後，而使置於該培養盒中的液體被傾倒至收集區33(S302)；該至少一填充裝置313將該培養盒進行填充；以該至少一開關裝置312將該培養盒的蓋子蓋上(S304)。收集完畢後，以該旋轉式夾具13從該第一輸送裝置311夾取該收集完成後的培養盒並輸送回該培養架11進行培養。Please refer to FIG. 3 to FIG. 5 and FIG. 10 , wherein the collecting process is mainly performed in the collecting area 33 of the working box 30 , 30A , and the step includes: placing the first conveying by the at least one switching device 312 The lid of the culture box of the device 311 is opened (S301). The first conveying device 311 rotates the incubator axially, so that the liquid placed in the incubator is poured into the collecting area 33 (S302); the at least one filling device 313 fills the incubator; At least one switching device 312 covers the lid of the incubator (S304). After the collection is completed, the collected cassette is taken from the first transport device 311 by the rotary jig 13 and transported back to the culture rack 11 for cultivation.
請參閱圖7所示，當其中一儲存箱體10A正在培養細胞而不需進行填充流程、觀察流程或收集流程時，其他儲存箱體10A內的培養盒可進入作業箱體30A進行填充流程、觀察流程或收集流程，使該作業箱體30A不會閒置。或是當該等儲存箱體10A都正在培養細胞而不需進行填充流程、觀察流程或收集流程時，三個儲存箱體10A可被置換成另外三個儲存箱體10A，使該作業箱體30A可持續進行不同流程而不會閒置。Referring to FIG. 7, when one of the storage cases 10A is culturing the cells without performing a filling process, an observation process, or a collection process, the culture boxes in the other storage cases 10A can enter the work box 30A for the filling process. Observe the process or the collection process so that the work box 30A is not left idle. Or when the storage tanks 10A are culturing the cells without performing a filling process, an observation process or a collection process, the three storage tanks 10A may be replaced with three other storage tanks 10A, so that the working compartments 30A can continue different processes without being idle.
以上經由本發明之細胞培養裝置的各個流程，可依需求各別執行或合併執行。The above various processes of the cell culture apparatus according to the present invention can be performed individually or in combination as needed.
根據本發明可作之不同修正及變化對於熟悉項技術者而言均顯然不會偏離本發明的範圍與精神。雖然本發明已敘述特定的較佳具體事實，必須瞭解的是本發明不應被不當地限制於二特定具體事實上。在實施本發明之已述模式方面，對於所屬領域中具有通常知識者而言顯而易知之不同修正亦被涵蓋於下列申請專利範圍之內。It is apparent to those skilled in the art that various modifications and variations can be made without departing from the scope and spirit of the invention. Although the present invention has been described in terms of specific preferred specific embodiments, it should be understood that the invention should not be Various modifications that are obvious to those of ordinary skill in the art are also intended to be included within the scope of the appended claims.
10、10A‧‧‧儲存箱體
11‧‧‧培養架
12‧‧‧直立軌道
13‧‧‧旋轉式夾具
14‧‧‧燈管
20、20A‧‧‧屏障
21‧‧‧第一閘門
30、30A‧‧‧作業箱體
31‧‧‧填充區
311‧‧‧第一輸送裝置
312‧‧‧開關裝置
313‧‧‧填充裝置
32‧‧‧觀察區
321‧‧‧第一觀察裝置
322‧‧‧第二輸送裝置
323‧‧‧第二觀察裝置
33‧‧‧收集區
40、40A‧‧‧滅菌箱體
41‧‧‧第二閘門
42‧‧‧紫外線滅菌裝置
50、50A‧‧‧資訊處理設備10, 10A‧‧‧ storage box
11‧‧‧Cultivator
12‧‧‧Upright track
13‧‧‧Rotary clamp
14‧‧‧Light tube
20, 20A‧‧‧ barrier
21‧‧‧The first gate
30, 30A‧‧‧Working cabinet
31‧‧‧filled area
311‧‧‧First conveyor
312‧‧‧Switching device
313‧‧‧Filling device
32‧‧‧ observation area
321‧‧‧First observation device
322‧‧‧Second conveyor
323‧‧‧Second observation device
33‧‧‧Collection area
40, 40A‧‧ ‧ sterilization cabinet
41‧‧‧second gate
42‧‧‧UV sterilizer
50, 50A‧‧‧Information processing equipment
圖1為本發明之細胞培養裝置之立體外觀圖。 圖2為本發明之細胞培養裝置之另一立體外觀圖。 圖3為本發明之細胞培養裝置之儲存箱體之立體外觀圖。 圖4為本發明之細胞培養裝置之作業箱體之俯視圖。 圖5為本發明之細胞培養裝置之作業箱體之填充區與收集區之立體外觀圖。 圖6為本發明之細胞培養裝置之作業箱體之觀察區之立體外觀圖。 圖7為本發明之另一細胞培養裝置之立體外觀圖。 圖8為本發明之使用細胞培養裝置的方法之填充流程之流程圖；S101至S103表示步驟101至步驟103。 圖9為本發明之使用細胞培養裝置的方法之觀察流程之流程圖；S201至S203表示步驟201至步驟203。 圖10為本發明之使用細胞培養裝置的方法之收集流程之流程圖；S301至S304表示步驟301至步驟304。Fig. 1 is a perspective view showing the cell culture apparatus of the present invention. Fig. 2 is another perspective view of the cell culture apparatus of the present invention. Fig. 3 is a perspective view showing the storage case of the cell culture apparatus of the present invention. Figure 4 is a plan view of the working box of the cell culture apparatus of the present invention. Fig. 5 is a perspective view showing a filling area and a collecting area of a working box of the cell culture apparatus of the present invention. Fig. 6 is a perspective view showing the observation area of the working box of the cell culture apparatus of the present invention. Fig. 7 is a perspective view showing another cell culture apparatus of the present invention. Fig. 8 is a flow chart showing a filling flow of the method of using the cell culture apparatus of the present invention; and S101 to S103 are shown as steps 101 to 103. Fig. 9 is a flow chart showing the observation flow of the method of using the cell culture apparatus of the present invention; S201 to S203 are shown as steps 201 to 203. Fig. 10 is a flow chart showing the collection flow of the method of using the cell culture apparatus of the present invention; and S301 to S304 are shown as steps 301 to 304.
10‧‧‧儲存箱體 10‧‧‧Storage cabinet
20‧‧‧屏障 20‧‧‧ barrier
30‧‧‧作業箱體 30‧‧‧Working cabinet
40‧‧‧滅菌箱體 40‧‧‧Sterilization cabinet
50‧‧‧資訊處理設備 50‧‧‧Information processing equipment
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