TWI538695B - Manufacturing method of dressing - Google Patents

Description

Method for manufacturing a material

The present invention relates to a method of producing a dressing material, and more particularly to a method of manufacturing a bacterial fiber dressing.

Bacterial cellulose film (BC film) is a water-insoluble and tough dense water film formed by fermentation of microorganisms. It is mainly composed of cellulose and water, and its moisture content can exceed 90%. The bacterial fiber membrane has the same chemical structure as natural cellulose, and thus can be widely applied to, for example, scald dressings and mask substrates.

Bacterial fiber membrane products are relatively more susceptible to microbial contamination than conventional non-woven substrate mask products. In general, in order to ensure the safety of the use of the bacterial fiber membrane product, the concentration of the preservative added to the product is increased. However, high levels of preservatives may cause irritation to the skin. To solve this problem, another way to preserve bacterial fiber membrane products is to store them at low temperatures (freezing).

However, although freezing can solve the above problem of preservative addition, the moisture in the bacterial fiber membrane product will precipitate out of the fiber membrane during the freezing and cooling process. surface. Especially when the ambient temperature of the freezing process is not low enough to quickly freeze the product, the effect of water analysis is more pronounced. Therefore, there is a need for a bacterial fiber membrane having a lower solidification temperature.

The present invention provides a method for producing a dressing material which can produce a dressing material which is resistant to low temperature, quick thawing, and low in freeze thawing weight change rate.

The present invention provides a method of manufacturing a dressing comprising the following steps. First, a bacterial fiber membrane is provided, which includes bacterial cellulose and moisture. Next, the bacterial fiber membrane is immersed in an aqueous glycerin solution and heated to more than 100 ° C to replace the glycerin in the aqueous glycerin solution with the water in the bacterial fiber membrane. Thereafter, a dialysis procedure is performed in which the temperature of the dialysis procedure is below room temperature such that glycerol exhibits a concentration gradient distribution in the bacterial fiber membrane.

In an embodiment of the invention, the thickness of the bacterial fiber membrane described above is D, and for example, D ≧ 2 mm.

In an embodiment of the present invention, in the method for producing a cast material, the content of glycerin in the aqueous glycerin solution is, for example, 50% by weight to 80% by weight based on the total weight of the aqueous glycerin solution.

In an embodiment of the present invention, in the method for producing a veneer, the temperature of the dialysis program is T, and T satisfies, for example, 0 ° C < T ≦ 26 ° C.

In an embodiment of the present invention, in the method for producing a cast material, the temperature of the dialysis program is T, and T satisfies, for example, 0 ° C < T ≦ 10 ° C.

In an embodiment of the invention, in the method of manufacturing the above-mentioned dressing, the time of the dialysis procedure is, for example, between 0 hours and 24 hours.

In an embodiment of the present invention, in the method for producing a cast material, the bacterial fiber membrane includes a surface layer and a center, and a concentration of glycerin exhibiting a concentration gradient distribution increases from a surface layer to a center of the bacterial fiber membrane.

In an embodiment of the present invention, in the method for producing a cast material, the average glycerin content of the glycerin exhibiting a concentration gradient distribution is, for example, 20% by weight to 60% by weight based on the total weight of the bacterial fiber membrane.

In an embodiment of the invention, in the method for producing a cast material, the bacterial cellulose has a fiber diameter of 20 nm to 100 nm.

In an embodiment of the invention, the method of manufacturing the above-described dressing further comprises the step of performing a freezing process wherein the temperature of the freezing process is less than 0 ° C and greater than -14 ° C. Next, a thawing procedure is performed in which the thawing procedure is performed for 10 minutes to 20 minutes.

Based on the above, the manufacturing method of the dressing material of the present invention has a simple process, and can produce a dressing material which is resistant to low temperature, quick thawing, and low in freezing and thawing weight change rate.

The above described features and advantages of the invention will be apparent from the following description.

10‧‧‧ surface

20‧‧‧ Center

S110, S120, S130‧‧‧ steps

1 is a schematic view showing a manufacturing process of a dressing material according to an embodiment of the present invention.

2 is a perspective view of a dressing material according to an embodiment of the present invention.

Figure 3 is a graph showing the glycerin concentration gradient of a dressing according to an embodiment of the present invention.

1 is a schematic view showing a manufacturing process of a dressing material according to an embodiment of the present invention. please Referring to Fig. 1, first, step S110 is performed to provide a bacterial fiber membrane. Specifically, the bacterial fiber membrane is, for example, a dense water gel film produced by static culture of acetic acid bacteria, but the present invention is not limited thereto. Bacterial fiber membranes include cellulose and water. It is worth mentioning that the bacterial fiber membrane of the present embodiment can produce a non-purified bacterial fiber membrane by a culture method, and then obtain a high purity bacterial fiber membrane by a lye alkaline washing procedure. The above lye may be selected from sodium hydroxide, sodium hypochlorite or sodium hydrogencarbonate, but the invention is not limited thereto. Further, the above bacterial fiber membrane has a dense pore structure and good tensile strength, and the fiber diameter of the bacterial cellulose is, for example, 20 nm to 100 nm, but the present invention is not limited thereto.

Next, in step S120, the bacterial fiber membrane is immersed in an aqueous glycerin solution and heated for about 20 minutes to raise the temperature to more than 100 °C. In the present embodiment, the glycerin content of the aqueous glycerin solution is, for example, 50% by weight to 80% by weight based on the total weight of the aqueous glycerin solution. This step is a solution replacement procedure in which glycerin of an aqueous glycerin solution is uniformly introduced into the bacterial fiber membrane. Commonly used antifreeze agents are, for example, methanol, ethanol, ethylene glycol, glycerin, water-soluble guanamine, calcium chloride, salt water, and the like. Since glycerin is non-toxic and highly safe, this embodiment preferably uses glycerin to lower the solidification temperature of the bacterial fiber membrane.

In particular, in the present embodiment, the thickness of the bacterial fiber membrane is D, and for example, D ≧ 2 mm, and D is preferably, for example, 2.0 mm to 5.0 mm. In general, the usual solution replacement is an immersion method. However, the solution displacement effect of the simple immersion method is often limited by the time, the thickness of the bacterial fiber membrane, and the concentration of the replacement solution. In particular, when the thickness D of the bacterial fiber membrane reaches a certain thickness (for example, 2 mm) or more, the above-described immersion method can only diffuse glycerin to the surface layer of the bacterial fiber membrane and cannot diffuse into the interior of the bacterial fiber membrane. As a result, the surface layer of the dressing will have a higher content of glycerin, which may cause the moisture of the skin to be absorbed by the high content of glycerin on the surface layer of the dressing, thereby causing the problem of dry skin of the user. In contrast, the method of manufacturing the dressing of the present embodiment achieves the purpose of replacement by heating to a temperature greater than 100 ° C and utilizing the difference in boiling point between glycerin and water. In this way, the glycerin can completely replace the moisture in the bacterial fiber membrane, and can be uniformly dispersed in the bacterial fiber membrane having a certain thickness, and the substitution manner does not affect the structure of the bacterial fiber membrane.

Further, in step S130, a dialysis procedure is performed at a temperature lower than room temperature to cause glycerol to exhibit a concentration gradient distribution in the bacterial fiber membrane. In the present embodiment, the temperature of the dialysis program is T, and T preferably satisfies 0 ° C < T ≦ 26 ° C, and T more preferably satisfies 0 ° C < T ≦ 10 ° C. In the above temperature range, glycerin is viscous and diffuses slowly, which is beneficial to the formation of a glycerol concentration gradient. In the present embodiment, the time of the dialysis procedure is, for example, between 0 hours and 24 hours.

2 is a perspective view of a dressing material according to an embodiment of the present invention. Referring to FIG. 2, the bacterial fiber membrane includes a surface layer 10 and a center portion 20, and exhibits a concentration gradient. The concentration of glycerin in the cloth increases from the surface 10 to the center 20 of the bacterial fiber membrane. In the present embodiment, the average glycerin content of the glycerin exhibiting a concentration gradient distribution is, for example, 20% by weight to 60% by weight based on the total weight of the bacterial fiber membrane. Figure 3 is a graph showing the glycerin concentration gradient of a dressing according to an embodiment of the present invention. Referring to FIG. 2 and FIG. 3 simultaneously, the center 20 of the material is regarded as 0 mm, and the horizontal axis is the distance (mm) from the center 20 to the surface layer 10. The larger the distance, the closer to the surface layer 10 of the material. The vertical axis is the glycerin content (% by weight) exhibiting a concentration gradient distribution. Referring to Figure 3, the glycerin concentration gradient of the dressing after the above dialysis procedure is increased from the surface 10 to the center 20 of the bacterial fiber membrane. Since the bacterial fiber membrane has a higher concentration of glycerin near the center 20, it does not freeze inside after freezing. In this way, the degree of appearance change after the thawing of the dressing of the embodiment can be reduced, and the thawing time of the dressing material and the function of releasing the water can be reduced.

It is worth mentioning that after the freezing and thawing process of the bacterial fiber membrane, the moisture contained in the membrane will cause the membrane water to be squeezed out due to the formation of ice crystals, which will cause the membrane of the membrane to be frozen and thawed. The appearance is affected. In contrast, since the dressing of the present embodiment contains glycerin having a concentration gradient, the above-described phenomenon of water analysis can be improved, and the weight recovery rate after the freezing and thawing process can be improved.

Evaluation of the characteristics of the material

Hereinafter, several comparative examples and experimental examples will be described to illustrate the freeze resistance, thawing time, freeze thawing weight recovery rate and water release efficiency of the dressing of the present embodiment.

[freezing resistance]

In detail, the evaluation method of frost resistance is the same as the comparative example and the experimental example. After storage for 14 hours at -14 ° C, the presence or absence of freezing was observed, and the results are shown in Table 1.

[thaw time]

Specifically, the thawing rate is evaluated by the above-mentioned sample of the comparative example and the experimental example after storage at -14 ° C for 24 hours, which is then thawed at room temperature, and the time required for complete thawing is measured, The results are listed in Table 1.

[weight recovery rate]

Specifically, the samples of the comparative examples and the experimental examples were stored at -14 ° C for 24 hours, and the weight Wa was measured. Subsequently, it was thawed at room temperature, and its weight Wb was measured. Herein, the freeze-thaw weight recovery rate (%) is used to quantify the appearance change of the frozen-thawed dressing. The weight recovery rate (%) is defined as: Wb/Wa × 100%, where Wa is the weight (mg) of the material before freezing and thawing, and Wb is the weight (mg) of the material after freezing and thawing, and the results are listed in the table. One. The higher the weight recovery rate, the smaller the change in appearance of the dressing.

[Water release efficiency]

Specifically, a 65% by weight gelatin film was used to simulate human skin, and a comparative sample and a sample of the experimental example were placed on a gelatin film for 15 minutes, and the difference in weight change of the film was compared. The water release efficiency (%) was defined as the gelatin weight change (mg) of the experimental example/the gelatin weight change (mg)/×100% of Comparative Example 2, and the results are shown in Table 1.

Comparative Example 1 is a commercially available jelly mask, and Comparative Example 2 is a bacterial fiber membrane containing no glycerin. Experimental Example 1 is a bacterial fiber membrane having an average glycerin content of >90% by weight, Experimental Example 2 is a bacterial fiber membrane having an average glycerin content of 60% by weight, and Experimental Example 3 is a bacterial fiber membrane having an average glycerin content of 40% by weight, Experimental Example 4 is a bacterial fiber membrane having an average glycerin content of 20% by weight, and Experimental Example 5 is a bacterial fiber membrane having an average glycerin content of 10% by weight. It should be noted that the bacterial fiber membranes of Experimental Examples 1 to 5 were subjected to a dialysis procedure at 4 °C.

From the results of Table 1, it can be seen that when the average glycerin concentration in the dressing of the present embodiment is higher than 60% by weight (ie, Experimental Example 1 and Experimental Example 2), it is still in the environment of 0 ° C to -14 ° C. Maintain a wet state for a long time without freezing. However, if the dressing is to be applied to a skin dressing such as a mask, in addition to considering the low temperature resistance of the dressing product, it is necessary to retain the water release efficiency of the product. Based on the consideration of the comprehensive factors, the average glycerin concentration in the dressing is preferably in the range of 10% by weight < average glycerin concentration ≦ 40% by weight (ie, Experimental Example 3 and Experimental Example 4). Specifically, when the average glycerin concentration of the dressing falls within the above range, although the dressing material is frozen, the weight recovery rate after thawing is ≧83%, and the thawing time is short (≦10 minutes). It is worth mentioning that the experimental example 3 and the real The dressing of the test case 4 can also maintain a good water release efficiency, which is 69.6% and 71.9%, respectively.

Further, in order to explain the influence of temperature on the effect of the dialysis program, several experimental examples are described below. Experimental Example 1 is a bacterial fiber membrane subjected to a dialysis procedure at 1 ° C, Experimental Example 2 is a bacterial fiber membrane subjected to a dialysis procedure at 4 ° C, and Experimental Example 3 is a bacterial fiber membrane subjected to a dialysis procedure at 10 ° C. It should be noted that the average glycerin content in the bacterial fiber membranes of Experimental Examples 1 to 3 was 40% by weight. The results are shown in Table 2.

As can be seen from the results in Table 2, as the temperature during the dialysis procedure increases, the water release efficiency of the dressing increases. In particular, the dressings subjected to the dialysis procedure at 10 ° C may have better water release performance (up to 75.4%). It should be noted that the lower the temperature, the more time is required for the dialysis procedure. For example, the equilibration time of the dialysis procedure at 10 ° C is about 1 hour to 2 hours, while the equilibrium time of the dialysis procedure at 1 ° C is about 2 hours to 3 hours.

In summary, the present invention utilizes the antifreeze property of glycerin, and uniformly introduces glycerin into the bacterial fiber membrane through a heating solution replacement procedure, and then controls the concentration of glycerol in the bacterial fiber membrane to form a concentration gradient by a dialysis procedure. Thereby, a veneer which is resistant to low temperature, quick thawing and has a small rate of change in freeze thawing weight is produced. In this way, the dressing of the present invention can be suitably stored at low temperatures without the need to add a high concentration of preservative.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and any one of ordinary skill in the art can make some changes and refinements without departing from the spirit and scope of the present invention. The scope of the invention is defined by the scope of the appended claims.

S110, S120, S130‧‧‧ steps

Claims (8)

A method for manufacturing a dressing comprising: providing a bacterial fiber membrane comprising bacterial cellulose and moisture; immersing the bacterial fiber membrane in an aqueous solution of glycerin and heating to greater than 100 ° C to complete the glycerin of the aqueous glycerin solution Displace the moisture in the bacterial fiber membrane, wherein the glycerin content of the glycerin aqueous solution is 50% by weight to 80% by weight based on the total weight of the glycerin aqueous solution; and performing a dialysis procedure, wherein the temperature of the dialysis procedure is T, and T satisfies 0 ° C < T ≦ 26 ° C, so that the glycerol exhibits a concentration gradient distribution in the bacterial fiber membrane. The method for producing a dressing according to claim 1, wherein the bacterial fiber membrane has a thickness D and a D ≧ 2 mm. The method for producing a dressing according to claim 1, wherein the temperature of the dialysis program is T, and T satisfies 0 ° C < T ≦ 10 ° C. The method of manufacturing a dressing according to claim 1, wherein the dialysis procedure is between 0 and 24 hours. The method for producing a dressing according to claim 1, wherein the bacterial fiber membrane comprises a surface layer and a center, and the concentration of the glycerin exhibiting a concentration gradient distribution is from the surface layer of the bacterial fiber membrane to the center Increase. The method for producing a dressing according to claim 1, wherein the glycerin exhibiting a concentration gradient distribution has an average glycerin content of from 20% by weight to 60% by weight based on the total weight of the bacterial fiber membrane. The method for manufacturing a dressing according to claim 1, wherein the The fiber diameter of the cellulose is from 20 nm to 100 nm. The method of manufacturing a dressing according to claim 1, further comprising: performing a freezing process, wherein the temperature of the freezing process is less than 0 ° C and greater than -14 ° C; and performing a thawing procedure, wherein the thawing procedure The time is 10 minutes to 20 minutes.