TWI472746B - Multiplex fiber optical biosensor and detection method by using the same - Google Patents

Description

Multiplex fiber optic biosensor and detection method

The invention relates to a biosensor and a detection method thereof, in particular to a multiplex fiber optic biosensor and a detection method thereof.

The fiber optic biosensor uses optical fiber to guide the light wave generated by the light source to the area to be tested. The physical quantity or chemical quantity in the area to be measured, such as changes in stress, strain, temperature, refractive index and molecular concentration, will cause light wave characteristics. The change of the physical or chemical quantity in the area to be tested can be derived by analyzing the change of the characteristics of the light wave. When the sensing signal of the fiber optic sensor is transmitted in the optical fiber, it is less immune to electromagnetic noise and magnetic field interference, and other effects such as free radiation can also be avoided by radiation treatment, so it is suitable for use in a strict environment, such as a nuclear power plant. The same fiber can be used as both a sensor and a signal transmission line. The overall volume of the sensor is often smaller than that of a conventional sensor plus a wire, so it can be placed in an area that is small or difficult to reach.

The optical fiber sensor uses light as the excitation and transmission medium, and does not use current and voltage, so there is no danger of electric shock, and it is suitable for medical measurement. The fiber material is not afraid of corrosion, suitable for deep sea engineering and chemically corrosive environments, and also has good biocompatibility. Glass fiber temperature resistance is better than metal strain gauges, long-term stability and fatigue life are higher than resistance strain gauges, suitable for long-term monitoring. Because fiber is used in long-distance communication, Therefore, fiber optic sensor related technology is easy to perform long-distance telemetry. In addition, the multiplexed technology of optical communication also contributes to multi-point measurement in the same fiber. Therefore, fiber optic sensors have been widely used in aerospace, medical, chemical, geotechnical, civil engineering, and other fields.

Please refer to FIG. 1 , which is a schematic diagram of a conventional optical fiber optic sensor. First, the multi-segment light sources λ ₁ , λ ₂ , λ ₃ ... λ _{n are} coupled into the optical fiber 100, and the light of different wavelengths is separated by the grating, the beam splitter group 110 or the prism, and then the photosensitive coupling element (Charge Coupled Device) is used. : CCD) or array type photo sensor accepts signals from different bands. However, such a conventional fiber optic biosensor has the following disadvantages: when the multi-segment light source is to be separated by the grating and the beam splitter group 110, if the wavelength is too close, the light source cannot be clearly separated, resulting in The error in the measurement. In addition, since the light sources are separated according to their respective wavelengths, it is necessary to have an equal number of detection units corresponding to the separated light sources. Therefore, when the analyte has a plurality of substances to be analyzed, the cost of the detection unit will increase. Moreover, this type of detection also requires the use of spectrometer analysis, which also causes an increase in instrument costs.

In recent years, the development of nano-materials has become the focus of research. The research and application of nano-materials, such as optoelectronics, energy and biomedical testing instruments, have been favored because of nano-materials. Nanomaterials provide properties that are completely different from those produced by the original material. The free electron cloud on the surface of precious metal nanoparticles can be excited by the electromagnetic field of a specific frequency, and then reacted by the phenomenon of collective dipole resonance. However, at this time, these active electron clouds are confined to the vicinity of the nanoparticle, so it is called Localized Surface Plasmon Resonance (LSPR), also known as particle plasma resonance (Particle Plasmon) Resonance: PPR). When the precious metal nanoparticles sense the change in the refractive index of the environment, the frequency and intensity of the particle resonance band of the particle will also change. When observing the absorption band of noble metal nanoparticles, it can be found that when the ambient refractive index rises, the absorption band of the particle plasma resonance will shift to the long wavelength, accompanied by the phenomenon of increased absorbance; By observing the characteristics, it is found that when the refractive index of the environment rises, the band of the scattered light also shifts to the long wavelength, accompanied by the phenomenon of enhanced light intensity. Finally, by modifying the specific identification unit to have a specific sensing capability, and by analyzing the relationship between the degree of change of the frequency and intensity of the resonant band and the concentration of the object to be tested, a method of checking can be established. The method mainly comprises modifying precious metal nanoparticles on an optical fiber to form a noble metal nanoparticle layer. The noble metal nanoparticle layer is composed of one of spherical noble metal nanoparticles, square noble metal nanoparticles, pyramidal noble metal nanoparticles, rod-shaped noble metal nanoparticles and shell noble metal nanoparticles, and The rice particles are basically not connected, and the precious metal is gold, silver or platinum. By utilizing the characteristics of multiple internal total reflection of the optical fiber, the variation of the evanescent wave absorption of the plasma resonance of the noble metal nanoparticle can be accumulated to increase the PPR signal to enhance the sensitivity of the sensing. When integrated with the identification unit, it has both specific and highly sensitive sensing capabilities, so it has the potential to be developed into sensing equipment for instant detection.

In view of the above, the object of the present invention is to provide a multiplex optical fiber optical biosensor and a detecting method thereof, which use light of different wavelengths to be incident into an optical fiber by using a different time series or a different carrier frequency signal. Finally, the detection unit detects and analyzes the variation of the particle plasma resonance signal.

The multiplex fiber optic biosensor of the present invention comprises: an optical fiber, a plurality of precious metals It is a nanoparticle layer, a plurality of light sources, and a light source function generator. The optical fiber includes a plurality of sensing regions, and the sensing regions are structures for removing the coating layer of the optical fiber to expose the optical fiber core, and different types of precious metal nano particle layers are disposed at the different sensing regions. In addition, the light sources respectively emit light of different wavelengths according to a function generated by the light source function generator in different time series or different carrier frequency signals, and the precious metal nano particle layers respectively absorb the different wavelengths. Light. When the light sources are incident on the optical fiber in different time series or different carrier frequency signals, the detecting unit detects the particle plasma resonance generated by the interaction between the precious metal nano particle layers and a test object. The amount of change in the signal.

Preferably, the multiplex fiber optic biosensor of the present invention further comprises a timing control unit, wherein the light source function generator is electrically connected to the timing control unit to cause the light sources to emit light in different time series.

Preferably, the light source function generator is electrically coupled to the detecting unit, and the function is transmitted to the detecting unit, and the triggering manner is used to analyze the variation of the particle plasma resonance signal of each distinct channel.

In addition, the detecting unit preferably includes a photodiode, a current amplifier, an analog digital converter, and a computer device. Among them, the photodiode system is used to excite the particle plasma resonance signal. In addition, a current amplifier is electrically connected to the photodiode for amplifying the particle plasma resonance signal. In addition, the analog digital converter is electrically connected to the current amplifier for converting the particle plasma resonance signal into a digital signal. Moreover, the computer device is electrically connected to the current amplifier for receiving and analyzing the particle plasma resonance signal.

In addition, the computer device preferably receives the particle plasma by a universal serial bus (USB). Resonance signal.

Still another object of the present invention is to provide a method for detecting a multiplex fiber optic biosensor comprising the steps of: providing an optical fiber and a plurality of noble metal nanoparticle layers, wherein the optical fiber comprises a plurality of sensing And the sensing regions are preferably structures for removing the cladding of the optical fiber to expose the core of the optical fiber, and the dissimilar noble metal nanoparticle layers are disposed at the different sensing regions. . Next, a light source function generator and a plurality of light sources are provided, wherein the light source function generator generates a function for causing the light sources to emit light according to the function in different time series or different carrier frequency signals, and the precious metals The nanoparticle layer absorbs light of different wavelengths, respectively. Next, a detecting unit is provided, wherein when the light of the light sources is incident on the optical fiber in a different time series or a different carrier frequency signal, the detecting unit is used to detect the precious metal nano particle layer and a test object. The particle plasma resonance signal generated by the interaction.

In the method for detecting the multiplex fiber optic biosensor of the present invention, the multiplex fiber optic biosensor preferably further includes a timing control unit, and the light source function generator is electrically connected to the timing control unit to The light sources are illuminated in a different time series.

Preferably, the light source function generator is electrically coupled to the detecting unit, and the function is transmitted to the detecting unit, and the triggering manner is used to analyze the variation of the particle plasma resonance signal of each distinct channel.

In addition, in the detecting method of the multiplex fiber optic biosensor of the present invention, the detecting unit preferably comprises: a photodiode, a current amplifier, an analog digital converter, and a computer device. Among them, the photodiode system is used to excite the particle plasma resonance signal. this In addition, a current amplifier is electrically connected to the photodiode for amplifying the particle plasma resonance signal. In addition, the analog digital converter is electrically connected to the current amplifier for converting the particle plasma resonance signal into a digital signal. Moreover, the computer device is electrically connected to the current amplifier for receiving and analyzing the particle plasma resonance signal.

In addition, the computer device preferably receives the particle plasma resonance signal by a universal serial bus (USB).

According to the above, the particle plasma resonance sensing device and the optical fiber structure of the present invention can have the following advantages:

(1) In the multiplex optical fiber optical biosensor of the present invention and the detection method thereof, it is not necessary to use a spectrometer to analyze the wavelength of light.

(2) In the multiplex optical fiber optical biosensor of the present invention and the detecting method thereof, it is only necessary to use a detecting unit to analyze various components in the object to be tested.

100‧‧‧ fiber

110‧‧‧Grating, beam splitter

21‧‧‧ fiber

211‧‧‧Sensing area

22‧‧‧ precious metal nanoparticle layer

23‧‧‧Silicon

24‧‧‧Stained shell

25‧‧‧Finishing

300‧‧‧ fiber

310‧‧‧Silicon

320‧‧‧Stained shell

330‧‧‧Sensing area

400, 401, 402‧‧‧ precious metal nano particle layer

403‧‧‧Silver Nanoparticles

404‧‧‧Ginna Bar

410‧‧‧Chemical structure for testing

411‧‧2,4 dinitrophenol

412‧‧‧Biotin

420‧‧‧Test object

500‧‧‧Light source function generator

501‧‧‧Sequence Control Unit

600‧‧‧Light source

601‧‧‧Light source drive unit

700‧‧‧Test wafer

710‧‧‧First carrier frequency sequence

720‧‧‧Second carrier frequency sequence

730‧‧‧ Third carrier frequency sequence

800‧‧‧Detection unit

810‧‧‧Photoelectric diode

820‧‧‧ Current amplifier

830‧‧‧ Analog Digital Converter

840‧‧‧ computer equipment

900, 910, 920‧ ‧ steps

Figure 1 is a schematic representation of a conventional fiber optic sensor.

2A is a schematic view showing an embodiment of the multiplexed fiber optic biosensor of the present invention for removing all of the looped fiber and the shell fiber.

FIG. 2B is a schematic diagram of an embodiment of a multiplexed fiber optic biosensor of the present invention with a portion of the looped fiber and the fiber of the shell.

2C is a cross-sectional view showing an embodiment of a multiplexed fiber optic biosensor of the present invention in which a portion of the looped fiber, the shell, and the core are removed.

3A to 3F are schematic views showing the fabrication of an optical fiber and a plurality of noble metal nanoparticle layers of the multiplex fiber optic biosensor of the present invention.

Figure 4A is a schematic diagram of the operation of the multiplexed fiber optic biosensor of the present invention.

Figure 4B is a schematic illustration of the illumination of such sources of the multiplexed fiber optic biosensor of the present invention in distinct time series.

Figure 4C is a schematic diagram of the detecting unit receiving the particle plasma resonance signal.

Figure 5A is a schematic illustration of the operation of another embodiment of the multiplexed fiber optic biosensor of the present invention.

FIG. 5B is a schematic diagram of the light sources of the multiplexed fiber optic biosensors of the present invention emitting light with different carrier frequency signals.

FIG. 5C is a schematic diagram of a detecting unit receiving a particle plasma resonance signal according to another embodiment of the multiplex fiber optical biosensor of the present invention.

Figure 6 is a schematic diagram of the experiment of the multiplexed fiber optic biosensor of the present invention.

Figure 7 is a graph of experimental results measured by means of a different time series in the present invention.

Figure 8 is a graph showing experimental results measured by means of a different carrier frequency signal.

Figure 9 is a step diagram of a method for detecting a multiplexed fiber optic biosensor of the present invention.

Please refer to FIG. 2A to FIG. 2C. FIG. 2A is a schematic diagram of an embodiment of the multiplexed fiber optic biosensor of the present invention for removing all of the looped fiber and the shell fiber. FIG. 2B is a schematic diagram of an embodiment of a multiplexed fiber optic biosensor of the present invention with a portion of the looped fiber and the fiber of the shell. 2C is a multiplexed optical fiber of the present invention A cross-sectional view of an embodiment of a biosensor that removes a portion of a looped fiber, a shell, and a fiber. In the 2A-2C diagram, the optical fiber 21 may include a core 23 and a cladding layer, and the cladding layer may include the fiber casing 24 and the fiber casing 25, and the optical fiber 21 may be a part or all of the annular fiber casing and The fiber of the fiber, and the fiber 21 includes a plurality of sensing regions 211, and the sensing region 211 is provided with a surrounding surface of the optical fiber 21. The sensing region 211 is a structure in which the cladding layer of the optical fiber 21 is removed to expose the core 23 of the optical fiber 21 .

In other words, the noble metal nanoparticle layer 22 is disposed at the sensing region 211, and may be composed of a gold nanoparticle, a silver nanoparticle or a white gold nanoparticle, and the nanoparticle composed of the noble metal nanoparticle layer 22 may be Pure nano particles. Wherein, when the light emitted by the light source is incident on the optical fiber 21, the detecting unit detects the interaction between the noble metal nanoparticle layer 22 and the object to be tested to generate a particle plasma resonance signal. Among them, the fiber 21 can be used as the sensing fiber 21 of the particle plasma resonance sensing device regardless of whether the fiber has the fiber 25. The sensing fiber 21 selected in the present invention may have a core 23 having a diameter of less than 1000 micrometers, and preferably the fiber core 23 may range from 4 to 400 micrometers.

Please refer to FIGS. 3A to 3F , which are schematic diagrams showing the fabrication of an optical fiber and a plurality of noble metal nanoparticle layers of the multiplex fiber optic biosensor of the present invention. First, the shells 320 are stripped in different sections of the optical fiber 300, the fibrils 310 are exposed to form a plurality of sensing regions 330, and different noble metal nanoparticle layers 400, 401 are respectively modified on the plurality of sensing regions 330, 402. The chemical structure 410 (eg, antigen or antibody) for detection is then modified on different layers of noble metal nanoparticles 400, 401, 402, depending on the type of analyte to be measured. Then, the object to be tested 420 having a complicated composition can be passed and detected. Among them, since the above-described chemical structure for detection 410 uses a substance that reacts only with a single component, it is possible to have specific adsorption.

When the aforementioned optical fiber 300 and precious metal nanoparticle layers 400, 401, 402 are ready, , that is, the analysis of the analyte can be performed. The multiplex fiber optic biosensor of the present invention can be analyzed mainly in two ways.

First, for the first way, please refer to Figures 4A to 4C. Figure 4A is a schematic diagram of the operation of the multiplexed fiber optic biosensor of the present invention. Figure 4B is a schematic illustration of the illumination of such sources of the multiplexed fiber optic biosensor of the present invention in distinct time series. Figure 4C is a schematic diagram of the detecting unit receiving the particle plasma resonance signal. First, the light source function generator 500 generates a function to cause the plurality of light sources 600 of different wavelengths to emit light in different time series. Preferably, the light source function generator 500 is electrically connected to a timing control unit 501, thereby calculating Elapsed time. In addition, the light source 600 is preferably electrically coupled to a light source driving unit 601, and the light source driving unit 601 is electrically coupled to the light source function generator 500 to receive a function, so that the light source 600 can emit light according to a function at different sequence times. For example, suppose that there are three components of the object to be tested that need to be analyzed. Therefore, three different lighting time points are needed. More specifically, for example, the first light source is at the 1st, 4th, 7th, and 10th. The second light source emits light in the second second, the fifth second, the eighth second, the eleventh second, etc., and the third light source is in the third second, the sixth second, the ninth second, the twelfth. Seconds...etc. It should be noted that here is an example of three components. For the sake of singularity, the three components need to correspond to three different wavelengths of light source, and the precious metal nanoparticle layer will separately absorb light of different wavelengths to achieve unity. The effect. In other words, three different wavelengths of light source emit light in three different time series and are respectively absorbed by three different layers of noble metal nanoparticles to produce a particle plasma resonance signal. Among them, it is particularly mentioned that light of three different wavelengths does not continuously emit light, but only emits light in the time series specified by the function.

In other words, the three kinds of light sources produced by the optical fiber and the noble metal nanoparticle layer are produced. The sub-plasma resonance signal is detected by the same detection unit. In particular, the applicant here refers to the fiber and noble metal nanoparticle layer as the detection wafer 700. Then, the light source function generator 500 is electrically coupled to the detecting unit 800 and transmits the function to the detecting unit 800. Therefore, the detecting unit 800 can correspondingly solve the light source resonance signal of the detected particle plasma according to the detected particle plasma resonance signal and function. Therefore, the multiplex fiber optic biosensor of the present invention not only needs to use one detecting unit 800, but also does not need to be identified by the spectrometer.

In addition, the multiplex fiber optic biosensor of the present invention further proposes a second mode, that is, the light emitted by the light source is incident into the optical fiber by a different carrier frequency signal, and a particle plasma resonance signal is generated.

Please refer to Figures 5A through 5C. Figure 5A is a schematic illustration of the operation of another embodiment of the multiplexed fiber optic biosensor of the present invention. FIG. 5B is a schematic diagram of the light sources of the multiplexed fiber optic biosensors of the present invention emitting light with different carrier frequency signals. FIG. 5C is a schematic diagram of a detecting unit receiving a particle plasma resonance signal according to another embodiment of the multiplex fiber optical biosensor of the present invention. In detail, the light source function generator 500 generates a function, so that the plurality of light sources 600 generate different carrier frequency signals according to the function, and the light source 600 is preferably electrically coupled to a light source driving unit 601, and the light source driving unit 601 The light source function generator 500 is electrically coupled to receive a function, so that the light source 600 can emit light with different carrier frequency signals according to a function. And the light source is incident on the detecting wafer 700, and interacts with the noble metal nanoparticle layer in the detecting wafer 700 and a test object to generate a particle plasma resonance signal. For example, suppose that there are three components of the object to be tested that need to be analyzed. Therefore, three different carrier frequency sequence illuminations are needed, for example, the first source is in the first carrier frequency order. Column 710 emits light, the second source emits light in a second carrier frequency sequence 720, and the third source emits light in a third carrier frequency sequence 730. It should be noted that in this mode, all three light sources are continuously illuminated, except that each carrier frequency sequence is different. Similarly, for the sake of singularity, the three components need to correspond to three different wavelengths of light source, and the precious metal nanoparticle layer will separately absorb the light of different wavelengths to achieve a single effect.

In other words, the particle plasma resonance signals generated by the three light sources after detecting the wafer 700 are detected by the same detecting unit 800. Then, preferably, the light source function generator 500 is electrically coupled to the detector. Unit 800 is measured and the function is transmitted to the detection unit. Therefore, the detecting unit 800 can correspondingly solve the light source resonance signal of the detected particle plasma according to the detected particle plasma resonance signal and function. Therefore, the multiplex fiber optic biosensor of the present invention can be used not only by using one detecting unit 800, but also without identifying the type of light by the spectrometer.

In order to prove that the multiplex fiber optic biosensor of the present invention has a definite effect, we have also proposed an experimental data for consideration. Please refer to FIG. 6. FIG. 6 is a schematic diagram of the experiment of the multiplex fiber optic biosensor of the present invention. First, silver nanoparticle 403 and gold nanorod 404 are respectively disposed on the sensing region 330 of the optical fiber 300, and then 2,4 dinitrophenol (DNP) 411 and biotin (412) molecules are respectively modified in silver. Nanoparticles 403 and gold nanorods 404.

Then, after detecting by the multiplex fiber optic biosensor of the present invention, the result of FIG. 7 can be measured in a different time series. Figure 7 is a graph of experimental results measured by means of a different time series in the present invention. When the anti-deoxyribonucleoprotein antibody (Anti-DNP) was introduced in 150 seconds, it was found that the silver nanoparticles were distinct. The signal changes in response, while the gold nanorod section does not change. Then, the buffer (PBS) was introduced at 475 seconds, and then streptavidin was introduced in 690 seconds, and it was found that there was a change in the section of the gold nanorod, and no change was observed in the silver nanoparticle segment. The feasibility of the multiplexed fiber optic biosensor of the present invention in a different time series manner.

In addition, please refer to FIG. 8 , which is a graph of experimental results measured by means of a different carrier frequency signal according to the present invention. After the multiplexed fiber optic biosensor of the present invention is detected, the result of FIG. 8 can be measured by means of a different carrier frequency signal. When the anti-deoxyribonucleoprotein antibody (Anti-DNP) was introduced at 210 seconds, it was found that there was a significant signal change reaction in the silver nanoparticle region, while the gold nanorod segment did not change. Then, the buffer (PBS) was introduced at 550 seconds, and then the streptavidin was introduced in 750 seconds, and it was found that there was a change in the gold nanorod segment, and the silver nanoparticle segment was unchanged, which confirmed The feasibility of the multiplexed fiber optic biosensor of the present invention is in the form of a different carrier frequency signal.

In addition, in the multiplex fiber optic biosensor of the present invention, the detecting unit 800 preferably includes a photodiode 810, a current amplifier 820, an analog digital converter 830, and a computer device 840. The photodiode 810 is used to excite the particle plasma resonance signal. The current amplifier 820 is electrically connected to the photodiode 810 for amplifying the optical signal variation or the particle plasma resonance signal. Then, the analog-to-digital converter 830 is electrically connected to the current amplifier 820. Since the received particle plasma resonance change signals are analog signals, if the particle plasma resonance signal is to be analyzed by the computer device 840, the analog signal needs to be converted. It is a digital signal. Thus, the analog to digital converter 830 converts the particle plasma resonance signal into a digital signal. Finally, the particle plasma resonance is received and analyzed via a computer device 840 electrically connected to the current amplifier 820. Signal. In addition, it is specifically mentioned that the computer device 840 preferably receives the particle plasma resonance signal via a universal serial bus (USB).

In addition, please refer to FIG. 9 , which is a step diagram of a method for detecting a multiplex fiber optic biosensor according to the present invention. The method for detecting a multiplex fiber optic biosensor of the present invention comprises the following steps: Step 900 provides a fiber and a plurality of layers of precious metal nanoparticles, wherein the fiber includes a plurality of sensing regions, and the sensing regions The structure for removing the fiber core of the fiber is exposed, and the precious metal nano particle layer is disposed at the sensing regions; then, step 910 is to provide a light source function generator and a plurality of light sources. Wherein the light source function generator generates a function for causing the light sources to emit light according to the function in different time series or different carrier frequency signals, and the precious metal nano particle layers respectively absorb the light of different wavelengths; and the steps The 920 system provides a detecting unit, wherein when the light sources are incident on the optical fiber in different time series or different carrier frequency signals, a detecting unit detects the interaction between the precious metal nano particle layers and a test object. A particle plasma resonance signal is generated.

The above is intended to be illustrative only and not limiting. Any equivalent modifications or alterations to the spirit and scope of the invention are intended to be included in the scope of the appended claims.

500‧‧‧Light source function generator

501‧‧‧Sequence Control Unit

600‧‧‧Light source

601‧‧‧Light source drive unit

700‧‧‧Test wafer

800‧‧‧Detection unit

810‧‧‧Photoelectric diode

820‧‧‧ Current amplifier

830‧‧‧ Analog Digital Converter

840‧‧‧ computer equipment

Claims (8)

A multiplex fiber optic optical biosensor includes: an optical fiber comprising a plurality of sensing regions, and wherein the sensing regions are structures for removing a cladding of the optical fiber to expose one of the fibers; a plurality of precious metal nanoparticle layers disposed in the sensing regions; a plurality of light sources, wherein the light sources respectively emit light of different wavelengths, and the precious metal nanoparticle layers respectively absorb light of different wavelengths; a light source function generator, wherein the light source function generator generates a function for causing the light sources to repeatedly emit light in different time series according to the function or to continuously emit light with different carrier frequency signals; wherein, when the light sources are When the light is repeatedly incident on the optical fiber by using a different time series or a different carrier frequency signal, a detecting unit detects a particle plasma resonance signal generated by the interaction between the precious metal nano particle layer and a test object, The detecting unit is electrically coupled to the light source function generator, and the light source function generator transmits the function to the detecting unit to analyze the particle plasma resonance signal The multiplex fiber optic biosensor of claim 1, further comprising a timing control unit, wherein the light source function generator is electrically connected to the timing control unit to make the light sources have different times The sequence repeats luminescence. The multiplexed optical fiber optical sensor of claim 1, wherein the detecting unit comprises: a photodiode for exciting the particle plasma resonance signal; and a current amplifier, the current amplifier system Electrically connecting the photodiode for placing a plasma oscillator resonance signal; an analog-to-digital converter electrically connected to the current amplifier for converting the particle plasma resonance signal into a digital signal; and a computer device The current amplifier is connected to receive and analyze the particle plasma resonance signal. The multiplexed fiber optic biosensor of claim 3, wherein the computer device receives the particle plasma resonance signal by a universal serial bus (USB). A method for detecting a multiplex fiber optic biosensor comprises the steps of: providing an optical fiber and a plurality of precious metal nanoparticle layers, wherein the optical fiber comprises a plurality of sensing regions, and the sensing regions are a cladding layer of the optical fiber such that a core of the optical fiber is exposed, and the precious metal nanoparticle layer is disposed at the sensing regions; a light source function generator and a plurality of light sources are provided, wherein the light source function The generator generates a function for causing the light sources to repeatedly emit light in different time series according to the function or to continuously emit light with different carrier frequency signals, and the precious metal nano particle layers respectively absorb light of different wavelengths; Providing a detecting unit, wherein when the light of the light sources is repeatedly incident on the optical fiber in a different time series or a different carrier frequency signal, the detecting unit is used to detect the precious metal nano particle layer and a test object The interaction generates a particle plasma resonance signal, wherein the light source function generator is electrically coupled to the detection unit, and transmits the function to the detection unit. To analyze the particle plasmon resonance signal. The method for detecting a multiplex fiber optic biosensor as described in claim 5, further comprising a timing control unit, wherein the light source function generator is electrically connected to the timing control unit to enable the light sources Illuminating in a different time series. The method for detecting a multiplex fiber optic biosensor as described in claim 5, wherein the detecting unit comprises: a photodiode for exciting the particle plasma resonance signal; and a current amplifier, The current amplifier is electrically connected to the photodiode for amplifying the particle plasma resonance signal; and an analog-to-digital converter electrically connected to the current amplifier for resonating the particle plasma The signal is converted into a digital signal; and a computer device is electrically connected to the current amplifier for receiving and analyzing the particle plasma resonance signal. The method for detecting a multiplex fiber optic biosensor according to claim 7, wherein the computer device receives the particle plasma resonance signal by a universal serial bus (USB).