TWI462756B - Method of using peracetic acid for inhibiting pathogenic microorganisms on animal farms - Google Patents

Description

Method for inhibiting pathogenic microorganisms in animal farms with peracetic acid

The present invention relates to a method of inhibiting pathogenic microorganisms in an animal farm using peracetic acid.

Traditionally, in the application of water treatment in animal husbandry, antibacterial agents have been used very early to carry out sterilization of diseases and environments for therapeutic animals such as pond water, animal breeding, aquaculture, etc. These antibacterial agents include β. - Beta-lactam, Aminoglycoside, Macrolide, Tetracycline, Chloramphenicol, Quinolone ), sulfonamides (Sulfonamides) and other drugs; among these categories of drugs, Taiwan's current approval for aquaculture animal drugs are Amoxicillin, Ampicillin, Doxycycline , Erythromycin, Florfenicol, Kitasamycin, Lincomycin, Oxolinic acid, Oxolinic acid derivatives Flumequine, Oxytetracycline, Spiramycin, Sulfamonomethoxine, Sulfadimeth, Sulfonamide Oxine), and sulfomycin (Thiamphenicol), etc.; however, in the case of animal farming, in the absence of knowledge of animal husbandry medication, especially the unidentified source of infection, any continuous application of the same class of drugs caused crossover The problem of drug resistance, and long-term use, causing pathogens to become generally resistant to most drugs, resulting in a continuous increase in the dose of the drug, and ultimately the result of invalid use of the drug; Further, the problem of drug residues caused by failure to comply with the withdrawal period regulations has caused people to threaten their health after eating animals. In addition, aquaculture water is discharged arbitrarily, causing serious pollution, including coastal waters and inland areas.

Taking animal breeding as an example, the use of chemical bactericides other than the above drugs is another option, but the commonly used quaternary amines have poor bactericidal power against Gram-negative bacteria and are highly toxic to algae which provide an important source of dissolved oxygen in the pool water; Aldehydes such as formaldehyde and glutaraldehyde are carcinogens and are prohibited for use in animal farms; the use of chlorine compounds such as hypochlorous acid produces chloroform carcinogens; iodine compounds such as iodine are irritating to fish and shrimp. Poor bactericidal power against some Gram-negative bacteria. Animal farms do require a safer and more versatile fungicide.

Peracetic acid has strong bactericidal power, broad-spectrum and biodegradable environmental affinity. It has been used in high-level disinfection of medical devices (including plastic products), and also used in food processing plants as solid surfaces. Disinfection and cleaning of containers and transfer lines.

The preparation of peracetic acid can be carried out by reacting a solution of acetic acid, hydrogen peroxide and water, or by reacting a guanamine compound such as tetraacetylethylenediamine (TAED) with an inorganic peroxide in water. The aqueous phase/oil phase of peracetic acid is 98:2, which is a hydrophilic material, but it has poor penetrability to hydrophobic substances, and too high a dose will cause fatal toxicity to aquaculture animals, and cannot be used at high doses. Overcome the penetration of the oil phase (for example, using a 15% peracetic acid solution produced by Solvay Interox SA of Belgium), the LC50 of the semi-lethal concentration of the seawater fish immersed for 24 hours is 98.7ppm (peracetic acid concentration is 14.8ppm) In addition, the hydrophobic substances in the pool water, including grease, also affect the bactericidal efficacy of peracetic acid. So far, there are few related research reports and literatures. And related commodities reported the use of acetic acid in the treatment of aquaculture pond water. Similarly, there is no report on the sterilization of acetic acid in culture ponds, animal breeding, etc.

In response to the needs of the art, the present invention discloses a method for inhibiting pathogenic microorganisms on the surface of water and/or environmental solids of an animal farm using peracetic acid, the method comprising administering to the pond water and/or environmental solid surface of the farm (1) The diluted peracetic acid is such that the concentration of peracetic acid in the pool water and/or on the surface of the environmental solid is 0.0005 to 4.9 ppm; and (2) the concentration is 0.001 to 60 times the concentration of the diluted peracetic acid. A surfactant comprising a carbon chain of at least 9 carbon atoms; wherein the contact between the diluted peracetic acid and the diluted surfactant to the farm pool water and/or the ambient solid surface is at least 1 minute. The surfactant comprising a carbon chain of at least 9 carbon atoms enhances the penetration of peracetic acid into hydrophobic materials in animal farms and thereby enhances the ability of peracetic acid to inhibit pathogenic microorganisms.

The present inventors have found that although peracetic acid having good hydrophilicity has good inhibitory ability against pathogenic bacteria, it has poor permeability to hydrophobic substances, but contains carbon of at least 9 carbon atoms at a concentration of 0.001 to 60 times the concentration of diluted peracetic acid. The surfactant of the chain enhances the bacteriostasis of peracetic acid by increasing the penetration of peracetic acid into the hydrophobic material. The source of peracetic acid can be obtained by a well-known organic chemical reaction, but is not limited to a solution of hydrogen peroxide, acetic acid and water, or can be produced by oxidation of acetaldehyde, ethyl chloroform or acetic anhydride under acid catalysis. The solution may also be obtained from an aqueous solution produced from an N-guanamine compound, a peroxide salt, and water.

Hydrophobic substances include culture pond water containing or containing feed fats and oils, feed agitation tanks, feed conveying pipes, feed tanks, and barns a solid surface, etc., a Gram-positive and Gram-negative bacteria containing a glycan of a peptide, a cell wall containing a chitin containing a nitrogen-containing polysaccharide, a skin of a poultry animal, and a fish, containing The chitin-like shell of the shrimp and the hydrophobic substance produced by the skin ulcer of the fish, but not limited to the materials and equipment mentioned above.

The conventional method to increase the concentration of peracetic acid can increase the antibacterial activity of peracetic acid itself and accelerate its antibacterial rate, but the toxicity of peracetic acid can cause respiratory irritation and skin irritation in livestock animals, especially at very low concentrations. Peracetic acid may also cause lethal toxicity to aquaculture animals, and too low concentrations of peracetic acid often do not effectively inhibit certain microorganisms. The present inventors have found that the use of a surfactant having a carbon chain of at least 9 carbon atoms at a concentration of from 0.001 to 60 times the concentration of the diluted peracetic acid promotes the bacteriostatic action of the diluted peracetic acid.

The following is an example of the application of the aquaculture farm, but the present invention is not limited to the embodiment, but can be applied to culture pond water, animal breeding, the environment of the related embodiments and the application data thereof are not described again.

In one embodiment of the invention, the concentration of peracetic acid used in the environment and equipment of the livestock farm is between 0.0005 and 18%.

In one embodiment of the invention, the peracetic acid can be a solution prepared by the reaction of hydrogen peroxide, acetic acid, water, or acid.

In one embodiment of the invention, peracetic acid can be a solution prepared by reacting a peroxide salt or hydrogen peroxide with an N-guanamine compound in water.

In one embodiment of the invention, the peracetic acid may be a solution of acetaldehyde, ethyl hydrazine, or acetic anhydride produced by oxidation.

The concentration used in the present invention is calculated in ppm per part per million.

In one embodiment of the invention, the surfactant is an anionic surfactant comprising a carbon chain of at least 9 carbon atoms, comprising at least 9 A nonionic surfactant of a carbon chain of a carbon atom, a zwitterionic surfactant comprising a carbon chain of at least 9 carbon atoms, or a mixture thereof.

In one embodiment of the invention, the anionic surfactant comprising a carbon chain of at least 9 carbon atoms comprises: an alkyl sulfate comprising a carbon chain of at least 9 carbon atoms, a carbon comprising at least 9 carbon atoms Chain polyoxyethylene ether sulfate, polyoxyethylene alkylphenyl ether sulfate containing carbon chain of at least 9 carbon atoms, alkyl phosphate containing at least 9 carbon atoms, containing at least 9 An alkyl phosphate salt of a carbon chain of a carbon atom, a polyoxyethylene alkylphenyl ether phosphate of a carbon chain containing at least 9 carbon atoms, a fatty acid salt of a carbon chain containing at least 9 carbon atoms, and at least 9 An alkyl sulfonate of a carbon chain of a carbon atom, an alkylbenzene sulfonate comprising a carbon chain of at least 9 carbon atoms, an α-olefin sulfonate comprising a carbon chain of at least 9 carbon atoms, comprising at least 9 An α-sulfonated fatty acid salt of a carbon chain of a carbon atom, a peroxycarboxylic acid comprising a carbon chain of at least 9 carbon atoms, an alkali metal salt of a peroxycarboxylic acid comprising a carbon chain of at least 9 carbon atoms, comprising at least a carboxylic acid of a carbon chain of 9 carbon atoms, a carboxylic acid alkali gold salt of a carbon chain containing at least 9 carbon atoms And their mixture.

In one embodiment of the invention, the non-ionic surfactant comprising a carbon chain of at least 9 carbon atoms comprises: a polyoxyethylene alkyl ether comprising a carbon chain of at least 9 carbon atoms, comprising at least 9 carbons A polyoxyethylene alkylphenyl ether of a carbon chain of an atom, a polyoxyethylene-polyoxypropenol containing a carbon chain of at least 9 carbon atoms, and a mixture thereof.

In one embodiment of the invention, the peroxycarboxylic acid comprising a carbon chain of at least 9 carbon atoms comprises peroxynonanoic acid, peroxydecanoic acid, peroxyundecanoic acid. (peroxyundecanoic acid), peroxydodecanoic acid or a mixture of them.

In one embodiment of the invention, the at least 9 carbon atoms are included The carboxylate of the carbon chain includes nonanoic acid, decanoic acid, undecanoic acid, dodecanoic acid, tridecanoic acid, nutmeg. Tetradecanoic acid, an alkali metal salt of the foregoing, or a mixture thereof.

In one embodiment of the present invention, a metal ion chelating agent is used to prevent catalytic decomposition of peracetic acid by metal ions in water, wherein the metal ion chelating agent comprises Ethylenediaminetetraacetic acid (EDTA) and a sodium salt thereof. Diethyltriaminepentaacetic acid (DTPA) and its sodium salt, polyphosphate, organophosphate, phosphate, polyacrylate, organophosphate, sodium gluconate, and mixtures thereof.

In one embodiment of the present invention, the surfactant may comprise a pH buffering agent such that the pH of the diluted peracetic acid and the diluted surfactant on the farm water and/or the environmental solid surface is between 6 and 11 .

In one embodiment of the invention, the pH buffer comprises a carboxylic acid having less than 9 carbon atoms and the pH of the diluted peracetic acid and the diluted surfactant on the surface of the farm water and/or environmental solids. Between 6-11.

In an embodiment of the present invention, the hydrophobic substance includes a culture pond water containing or containing residual feed oil, a feed agitation tank, a feed conveying pipe, a feed supply tank, and a solid surface contained in the farm field, and the like. The oil contained in the skin of animals and fish, the chitin contained in the shell of shrimps, and the hydrophobic substances produced by skin ulcers of fish, etc., the cell wall of Gram-positive bacteria and Gram-negative bacteria The chitin of the nitrogen-containing polysaccharide of the cell wall of sugar and fungal microorganisms, but is not limited to the materials mentioned above and the contaminated equipment.

It is a good idea to use peracetic acid to inhibit pathogenic microorganisms in pool water. In the method, the aqueous phase/oil phase of peracetic acid has a ratio of 98:2, which is a hydrophilic substance, but has poor permeability to hydrophobic substances. There are many hydrophobic substances in the aquaculture pond water that affect the bactericidal power of peracetic acid, such as feed fats and oils, and fish oil coated with fat-soluble drugs used in the treatment of fish and shrimp diseases. They are the most common hydrophobicity in pool water. Source of matter; the hydrophobic material in poultry farms comes from feed oils, which prevents hydrophilic peracetic acid from coming into contact with microorganisms in the pool water, thereby reducing the bactericidal effect; when bacterial skin diseases occur, such as hydrophilic Aeromonas (Aeromonas hydrophila), Vibrio spp., and Flexibacter & Cytophaga often cause bacterial skin abscess in grouper, and fat and hyperplastic mucus in skin and muscle are obstacles to contact between acetic acid and fish. In addition, when the shrimp and crab have symptoms of surface shell, the long-chain polysaccharide containing hydrophobic chitin in the outer shell is also a barrier to the contact between peracetic acid and shrimp and crab; in addition, the toxicity characteristic of peracetic acid to aquatic animals is to increase the dosage. limits.

In fact, when the bactericidal power of acetic acid was tested in 2 x 10 ⁵ of Vibrio alginolyticus in seawater, it was found that the peracetic acid concentration increased by 10 times to 0.15 ppm from 0.015 ppm, and Vibrio alginolyticus could not be killed within 2 hours. However, if the concentration of peracetic acid is 0.125ppm, it will cause the 2 carp fry of the grouper to sink into the bottom of the pool and cannot rise. The pathological anatomy can be found that the ankle and liver are severely damaged, and then die rapidly. Although the long carbon chain surfactant is highly irritating to fish and shrimp, the present inventors have found that the use of a surfactant comprising a carbon chain of at least 9 carbon atoms and peracetic acid can be safely and effectively removed at a suitable soaking time. Pathogenic microorganisms in pool water; for example, in a seawater tank of 2吋 half fish fry, after adding a dilution of 0.05ppm peracetic acid solution to a sodium dilauryl sulfate diluted to a concentration of 0.6ppm 12 times peracetic acid, it can be used within 6 hours. The Vibrio alginolyticus was completely killed, and the pool water was changed after 6 hours, and the fish body was not damaged.

In addition, bacterial diseases often occur in aquaculture animals from the microscopic point of bacterial structure, such as: Clostridium botulinm (botulinum bacillus), Renibacterium salmoninarum (bacterial kidney disease; Bacterial Kidney Disease/) BKD), Staphylococcus sp. (staphylococcus; eye disease), Streptococcus sp. (streptococcus), Enterococcus/Lactococcus sp. (streptomycin), Lactobacillus sp. (pseudylosis), Mycobacterium marinum/Mycobacterium sp. Mycobacteria), Nocardia asteroids/Nocardia kampachi (Norcaosis), Staphylococcus epidermidis (S. epidermidis), etc.; from Gram-negative bacteria such as: Flexibacter/Cytophaga/Flavobacterium (ricky; columnar disease) Cold water disease), Edwardsiella ictaluri, E. tarda, Yersinia ruckeri, Citrobacter spp., Pasteurella piscicida (Photobacterium damsela subsp. piscicida, Pasteurella) , Serratia sp., sepulmonas fluorescens, Ps. Anguilliseptica . Vibrio sp (Vibrio Disease), Aeromonas salmonicida (furunculosis), Aer.hydrophila (eel red fin disease; hemorrhagic septicemia), Aer.Sobria / Chlamydiaceae / Rickettsiales (absolute endoparasitic; sepsis) and the like. The cell walls of these Gram-positive and Gram-negative bacteria contain hydrophobic peptide glycan glycosides, of which Gram-positive bacteria have thicker cell walls and contain high levels of peptide peptidoglycan; fungal cell walls contain The chitin of the nitrogen-containing polysaccharide; the bacterial wall of the mycobacteria and the nucleus bacteria contains a long chain of waxy properties of up to 20 to 30 carbons, making it a layer relative to the hydrophilic peracetic acid. Hydrophobic barrier, in which mycobacteria have been found to cause serious infections in alfalfa farms; common fish skin disease Such as: (1) freshwater and brackish water fish often appear fungus-like watery mildew Oomycetosis (Achlya and Saprolegnia) at 12 ° C to 18 ° C water temperature, will adhere to the surface of the sticky sticky cotton; (2 ) Vibrio, which is a Gram-negative bacterium, causes groupers and plaques including white-spotted symptoms such as gold mites, seven-star mites and California cockroaches, including pathogens and mucus, among which grouper 2 carp farms are highly dense. Breeding, and changing the water ratio every 2 hours, each time changing water is the beginning of a new infection, making Vibrio skin disease more difficult; (3) Atypical gas producing unit of Gram-negative bacteria Infected with bacteria, the symptoms are symptoms of skin ulcers. The above skin symptoms are characterized by sticky cotton batt (water mold), bacteria releasing protein peptides, protein peptides produced by the decomposition of muscle protein, and common skin ulcer characteristics such as mucus of oil and body surface hyperplasia. Microorganisms in the water cause infection on the surface of the fish, while live feeds such as red-tailed green and brine shrimp larvae are fed at the fry stage, and even the next fish that feed on carnivorous farmed fish may carry pathogenic bacteria, which may cause the fish to be in vivo from the body. Symptoms of infection in the table. Traditionally, feeding antibiotics and using antibiotics for medicated baths is a common method. However, most of the bacteria cause serious drug resistance due to long-term use. Even if the dosage is increased, the bacteria cannot be killed, and especially the fry and shrimps that have been soaked with antibiotics. Poor, when stocked to an outdoor fish pond, it is easy to cause maladaptation and death. In solving these problems, it is a good method to use probiotics in pool water to decompose organic waste with a beneficial amount of beneficial bacteria and inhibit the growth of pathogenic bacteria, but a large number of probiotics will become carriers of virus survival in water, and then The opportunity to promote viral infection makes the use of probiotics a useful and harmful conflicting practice. Peracetic acid is much less toxic to fish, shrimp and algae in water than other fungicides. It produces harmless acetic acid, water and oxygen after degradation. It is suitable for sterilization and purification in culture ponds. However, pool water includes oil and water. Gram-positive and Gram-negative bacteria of the glycopeptide of the peptide, the chitin fungus containing the nitrogen-containing polysaccharide on the cell wall, the shell of the shrimp containing the chitin, and the hydrophobicity of the skin ulcer of the fish Substances are the main cause of the inability to perform peracetic acid (the concentration of which is too toxic to animals in water) at a working concentration of at most a few ppm.

Bacterial diseases often occur in poultry-bred animals, such as: Staphylococcus aureus (Staphylococcus aureus), Streptococcus faecalis (E. faecalis), etc. from Gram-negative bacteria such as: Pssuedomonas Aeruginosa, Pseudoella typhimurium, Salmonella choleraesuis, Bacillus cereus, E. coli, etc.; Cocci, Trichophylton mentagrophytes, Aspergillus fumigatus, A. flavus, Penicillium sp., Scopulariopsis sp. Dependent), etc., these bacteria also contain the peptide glycopeptide in the cell wall, and the grease on the surface of the environment is a hydrophobic substance that can affect the bactericidal power of acetic acid.

The present invention uses a carbon chain intercalating agent comprising at least 9 carbon atoms to enhance penetration of the above hydrophobic materials in an animal farm, thereby enhancing the bactericidal power of peracetic acid to inhibit pathogenic microorganisms in animal farms.

To further explain the technical means and efficacy of the present invention for achieving the intended purpose of the invention, the method and the application thereof for inhibiting pathogenic microorganisms in animal farms by using peracetic acid according to the present invention, and the specific embodiments and characteristics thereof Efficacy, detailed description as follows.

The following is an application of the aquaculture farm as an example, but the present invention is not limited to the embodiment, but can be applied to culture pond water, animal breeding, poultry breeding, and the environment of the related embodiments and application data thereof will not be described again.

The following examples are merely illustrative of the invention and are not intended to limit the scope of the invention. Any modifications to this embodiment are still within the scope of the invention in the spirit of the invention.

<Example 1>

Peracetic acid diluted to a concentration of 0.045 ppm in seawater was used as a control group, and the other experimental group was treated with peracetic acid which was also diluted in seawater at a concentration of 0.045 ppm and diluted in seawater at a concentration of peracetic acid 40. A surfactant of 1.8 ppm, sodium lauryl sulfate, is used to treat white plaques caused by Vibrio infection on the spotted grouper (brown spot) of about 8:30 in length. The method was to soak the diseased fish in two groups of sea water solution, the soaking time was 12 hours, and the white plaque on the skin of the fish was observed after soaking for 3 times in the same manner. The results showed that the white plaque on the skin of the diseased fish that had been soaked three times did not change significantly in the control group; the white plaque on the skin of the diseased fish that had been soaked in another experimental group with sodium lauryl sulfate was soaked for the third time. After the white plaque disappeared.

<Embodiment 2>

In the sea water tank where 2 吋 half of the fry are cultured, the sea water temperature is 22.5 ° C, the pH value is 8.3, the salt is 35 ⁰ / ₀₀ , and the diluted 0.05 ppm peracetic acid solution is added to the diluted 12-fold peracetic acid concentration of 0.6 ppm. After a surfactant, sodium lauryl sulfate, Vibrio alginolyticus can be completely killed within 6 hours, and the water is changed after 6 hours, and the fish body is not damaged.

<Example 3>

Preparation of A, B and C three groups of surfactants containing different components of the peracetic acid sterilizing aqueous solution, Group A containing 37.7mg / L of peracetic acid, Group B containing 37.7mg / L of peracetic acid, 88mg / L of dodecane Sodium benzenesulfonate, and 47 mg/L of polyoxyethylene lauryl ether. Group C contained 18.8 mg/L of peracetic acid, 16.1 mg/L of peroxydecanoic acid, 88 mg/L of sodium dodecylbenzenesulfonate, and 47 mg/L of polyoxyethylene lauryl ether. The killing rate of three groups of sterilizing liquid against Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans in the amount of 5x10 ⁶ cfu/ml was tested at 20 °C.

Test methods: Pseudomonas aeruginosa and Staphylococcus aureus were tested according to EN 1276, and Candida albicans was tested according to EN 1650.

The results are as follows (1), (2), (3):

(1) Pseudomonas aeruginosa (genus Gram-negative bacteria):

(2) Staphylococcus aureus (genus Gram-positive bacteria):

(3) Candida albicans (genus fungi):

It can be found from the above table (1), (2) and (3) that it contains a surfactant. The bactericidal liquid, especially for the gram-negative bacteria Pseudomonas aeruginosa, the gram-positive bacteria Staphylococcus aureus and the fungus-like Candida albicans, have a stronger bactericidal power.

Claims (11)

A method of inhibiting pathogenic microorganisms in an animal farm comprising administering to a farm pond water and/or an environmental solid surface: (1) diluted peracetic acid, such that the farm pool water and/or environmental solid surface a peracetic acid concentration of 0.0005 to 4.9 ppm; and (2) a surfactant having a carbon chain of at least 9 carbon atoms at a concentration of 0.001 to 60 times the concentration of the diluted peracetic acid; wherein the administration is at least 1 minute Thereafter, the surfactant comprising a carbon chain of at least 9 carbon atoms enhances the penetration of the peracetic acid into the hydrophobic material in the animal farm and thereby enhances the ability of the peracetic acid to inhibit the pathogenic microorganism. The method of claim 1, wherein the diluted peracetic acid concentration in the pool water of the livestock farm is 0.0005 to 18%. The method of claim 1, wherein the surfactant comprising a carbon chain of at least 9 carbon atoms is an anionic surfactant comprising a carbon chain of at least 9 carbon atoms, comprising at least 9 carbon atoms. A non-ionic surfactant of a carbon chain, or a mixture thereof. The method of claim 3, wherein the anionic surfactant is selected from the group consisting of alkyl sulfates, polyoxyethylene ether sulfates, polyoxyethylene alkylphenyl ether sulfates, alkyl phosphates, and alkane. Phosphate salt, polyoxyethylene alkylphenyl ether phosphate, fatty acid salt, alkyl sulfonate, alkyl benzene sulfonate, α-olefin sulfonate, α-sulfonated fatty acid salt, carboxylic acid, including a carboxylic acid alkali metal salt of a carbon chain of at least 9 carbon atoms, a peroxycarboxylic acid, an alkali metal salt of a peroxycarboxylic acid containing a carbon chain of at least 9 carbon atoms, or a mixture thereof. The method of claim 3, wherein the nonionic surfactant is selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyethylene alkyl phenyl ethers, polyoxyethylene-polyoxypropylene alcohols, or a mixture thereof. . The method of claim 3, wherein the surfactant comprises a pH buffering agent such that the pH of the diluted peracetic acid and the diluted surfactant on the farm water and/or the environmental solid surface is between 6 and 11. The method of claim 6, wherein the pH buffer comprises a carboxylic acid having less than 9 carbon atoms, and the diluted peracetic acid and the diluted surfactant are on the surface of the farm water and/or the environmental solid. The pH is between 6-11. The method of claim 4, wherein the peroxycarboxylic acid is selected from the group consisting of peroxynonanoic acid, peroxydecanoic acid, peroxyundecanoic acid or Peroxydodecanoic acid or a mixture of these. The method of claim 4, wherein the carboxylic acid is selected from the group consisting of nonanoic acid, decanoic acid, undecanoic acid, decanoic acid, thirteen A mixture of tridecanoic acid or tetradecanoic acid or a mixture thereof. The method of claim 1, wherein the hydrophobic substance comprises feed fats, oils on the surface and muscles of fish and poultry animals, chitin contained in shells of shrimps and crabs, and Gram-positive The cell wall of the bacterium and the Gram-negative bacterium contains the peptide glycan, and the chitin of the nitrogen-containing polysaccharide of the cell wall of the fungal microorganism. The method of claim 1, further comprising using a metal ion chelating agent to prevent catalytic decomposition of peracetic acid by metal ions in the water, wherein the metal ion chelating agent is selected from the group consisting of Ethylenediaminetetraacetic acid (EDTA). Or its sodium salt, diethyltriaminepentaacetic acid (DTPA) or its sodium salt, polyphosphate, organophosphate, phosphate, polyacrylate, Organic phosphate, sodium gluconate, or a mixture of them.