TWI443192B - Device for cell lysis and homogenization and method thereof - Google Patents

Description

Cell lysis homogenization device and method thereof

The present invention relates to cell lysis homogenization technology, and more specifically, to a cell lysis homogenization device capable of rapidly lysing a sample with high and low oscillation frequency and preventing sample nucleic acid or protein denaturation and method.

As shown in FIG. A, the conventional cell lysis homogenization operation first places the sample 1 to be tested together with the plurality of magnetic beads 2 in a test tube 3, and drives the test tube 3 by means of a machine to the test tube by tapping. The magnetic beads 2 produce oscillating grinding. As shown in the first B diagram, the cell lysis homogenization action starts from the contact surface of the sample 1 to be tested and the magnetic beads 2, and the sample 1 to be tested is gradually ground and lysed into a small shape. Until the entire sample 1 to be tested is ground and lysed into a uniform fine shape by the magnetic beads 2, the cell lysis homogenization action of the sample 1 to be tested is completed.

Since the machine of such an application generally uses a motor to drive a bar at a fixed speed to knock the outside of the test tube 3, the motor rotation speed and the bar tapping frequency are proportional to the frequency of the magnetic bead 2 oscillation. When the motor speed is fast, the oscillation frequency and the oscillation amplitude of the magnetic bead 2 are large, and the cell lysis homogenization action of the sample 1 to be tested can be quickly completed, but the greater the frequency and amplitude of the magnetic bead 2 oscillation, the more energy the sample 1 receives. Many, resulting in an increase in the overall temperature, causing the sample 1 to be tested due to high temperature, resulting in nucleic acid or protein denaturation, and can not be subsequently analyzed. Conversely, when the motor speed is slow, the frequency and amplitude of the magnetic bead 2 are small, which is not easy to cause denaturation of nucleic acid or protein, but it is therefore too long for the sample to be tested to reach homogenization.

Although another conventional cell lysis homogenization machine can manually adjust the motor to a high and low speed, but the fixed speed is still adjusted before and after the adjustment, and the user needs to judge the time point of the speed switching, it is still easy to cause the sample due to the user operation. Produces nucleic acid or protein denaturation defects.

In general, the shortcomings of the prior art are generally included in the conventional cell lysis homogenization action system at a fixed rotational speed and frequency, which is susceptible to frequency control, resulting in problems such as denaturation or cleavage of nucleic acids or proteins for too long. In view of the above shortcomings, the present invention proposes a cell lysis homogenization device and a method thereof, which has the advantages of automatically performing cell lysis homogenization at a non-fixed rotation speed and frequency, ensuring no nucleic acid or protein denaturation and short processing time. .

The invention provides a cell lysis homogenization method comprising the following steps:

a) preparing at least one test tube for accommodating the sample to be tested and at least one grinding unit;

b) tapping the test tube at a non-fixed frequency;

c) Using the grinding unit to generate high and low frequency oscillations during tapping, and perform cell lysis homogenization action on the sample to be tested.

Furthermore, the present invention further provides a cell lysis homogenization apparatus, comprising: at least one tapping rod, the striking rod is driven by a rotating motor to accommodate at least one sample to be tested and a plurality of grinding units The test tube generates a percussion oscillation; at least one variable frequency control module controls the rotating motor to drive the striking rod at a non-fixed speed, and generates a non-fixed tapping frequency to the test tube to drive the grinding unit to generate high and low frequency oscillations. The sample to be tested is subjected to cell lysis homogenization.

The main purpose of the invention is to make the tapping rod generate a non-fixed tapping frequency to the test tube by using the variable frequency rotation speed control module, and use the grinding unit to generate high and low frequency oscillation and the amplitude of the oscillation amplitude, so that the grinding unit has a high grinding amount and can be quickly The sample to be tested is subjected to cell lysis homogenization, and the energy received by the sample to be tested is controlled to ensure that the temperature of the sample is controlled within a safe value, thereby eliminating the problem of nucleic acid or protein denaturation.

The secondary object of the present invention is that the variable frequency speed control module can drive the rotating motor to generate a speed change according to a predetermined input speed control program. It is convenient for users to select the applicable speed control program according to different sample characteristics to ensure the same operation result and will not be different due to the operator's proficiency.

For the convenience of the description, the central idea expressed in the column of the above summary of the present invention is expressed by a specific embodiment. The various items in the embodiments are drawn to scale as illustrated and not to the actual proportions of the elements.

As shown in the second panel, the present invention provides a cell lysis homogenization method comprising the following steps:

a) preparing at least one test tube for accommodating the sample to be tested and at least one grinding unit;

b) tapping the test tube at a non-fixed frequency;

c) Using the grinding unit to generate high and low frequency oscillations during tapping, and performing cell lysis homogenization action on the sample to be tested.

To achieve the above method, as shown in the second to sixth figures, the present invention further provides a cell lysis homogenizing apparatus 10, which comprises:

A housing 20 is provided with a hollow receiving cavity 21, and an opening 22 is defined in the receiving cavity 21.

A fixing frame 30 is disposed at the opening 22, and the fixing frame 30 is provided with a plurality of through holes 31 for fixing the test tube 90.

The cover 40 is disposed at the opening 22 so that the cover 40 can be covered by the opening 22. The cover 40 is provided with a plurality of elastic members 41 corresponding to the through holes 31.

At least one tapping bar 50 is driven by a turning motor 51 to generate a tapping shock to the test tube 90 placed at the holder 30. In the preferred embodiment, the rotating motor 51 includes a rotating shaft 511 provided with a horizontal turntable 512. The striking rod 50 is fixed on the horizontal turntable 512, and the striking rod 50 and the rotating motor 51 are disposed in the receiving portion. The cavity 21 is located below the holder 30. The height of the bottom of the test tube 90 placed at the fixing frame 30 is lower than the height of the top end of the striking rod 50. And at least one variable speed control module 60 for controlling the rotating motor 51 to drive the striking rod 50 at a non-fixed rotational speed to generate a non-fixed tapping frequency to the test tube 90. In a preferred embodiment, the variable speed control module 60 can be a microcomputer or a single chip disposed at the housing 20 for controlling the program according to a predetermined input speed to drive the rotating motor 51 to generate a non-fixed rotational speed change.

After clarifying the detailed structure of the above case, the following is a detailed explanation of the action principle of the case:

As shown in the sixth drawing, the test sample 91 and the plurality of grinding units 92 are placed in the test tube 90 in advance, and the test tube 90 is closed and placed in the through hole 31 of the holder 30, and the elastic member 41 is pressed. Combined with the upper end of the test tube 90, the variable speed control module 60 drives the rotary motor 51 to generate a non-fixed rotational speed change.

The polishing unit 92 can be at least one of a plurality of magnetic beads, iron beads, bead beads, grinding beads, and an abrasive. And the plurality of magnetic beads, iron beads, bead beads, and abrasive beads can be a combination of different diameters, for example, a combination of 1 mm and 2 mm diameter.

Since the height of the bottom of the test tube 90 placed at the fixing frame 30 is lower than the height of the top end of the striking rod 50, when the horizontal turntable 512 of the rotating motor 51 rotates, the tapping rod 50 thereon will generate a non-deformation to the test tube 90. The fixed frequency tapping action, in turn, drives the grinding unit 92 in the tube 90 to produce high and low frequency oscillations.

As shown in FIG. 7A, since the grinding unit 92 continuously oscillates at different high and low frequencies, the grinding unit 92 jumps and the amplitude of the oscillation is large and small, so that the grinding unit 92 is rapidly distributed in a large solid shape to be tested. Between the samples 91, the contact space between the grinding unit 92 and the sample to be tested 91 is increased. As shown in the seventh B to the seventh C, the grinding unit 92 can quickly lyse the sample 91 cells into a homogeneous state, so as to shorten the time required for the treatment.

Moreover, since the grinding unit 92 oscillates with different frequency changes of continuous height, the sample to be tested 91 receives less energy transfer when the cell lysis homogenization operation is performed at a higher rotation speed than the conventional technique, and the temperature is too high, nucleic acid or protein can be avoided. The problem of transsexuality. In addition, the high speed can be retained to achieve the advantages of cell lysis homogenization.

Moreover, the variable frequency speed control module 60 of the present invention can drive the rotation motor 51 to generate a rotation speed change according to a predetermined input speed control program. It is convenient for users to select the applicable speed control program according to different sample characteristics to ensure the same operation result and will not be different due to the operator's proficiency.

Although the present case is illustrated by a preferred embodiment, those skilled in the art can make various forms of changes without departing from the spirit and scope of the present invention. The above embodiments are only used to illustrate the present case and are not intended to limit the scope of the present invention. All kinds of modifications or changes that are not in violation of the spirit of the case are the scope of patent application in this case.

10. . . Cell lysis homogenization device

20. . . case

twenty one. . . Cavity chamber

twenty two. . . Opening

30. . . Fixing frame

31. . . perforation

40. . . Cover

41. . . Elastic component

50. . . Knocker

51. . . Rotating motor

511. . . Rotary axis

512. . . Horizontal turntable

60. . . Frequency conversion speed control module

90. . . test tube

91. . . Sample to be tested

92. . . Grinding unit

The first A map ~ the first C map is a schematic diagram of the action of the conventional cell lysis homogenization.

The second figure is a schematic flow chart of the cell lysis homogenization method of the present invention.

The third figure is a block diagram showing the composition of the cell lysis homogenization apparatus of the present invention.

The fourth figure is a schematic diagram of the three-dimensional combination of the cell lysis homogenization apparatus of the present invention.

The fifth figure is a schematic exploded view of the cell lysis homogenization apparatus of the present invention.

Figure 6 is a schematic cross-sectional view of the cell lysis homogenization apparatus of the present invention.

7A to 7C are schematic diagrams showing the action of homogenization of cell lysis according to the present invention.

Claims (7)

A cell lysis homogenization device comprises: a casing having a hollow accommodating cavity therein; an opening is arranged above the accommodating cavity; a fixing frame is disposed at the opening, and the fixing frame is provided a plurality of perforations for respectively fixing a test tube in which at least one sample to be tested and at least one grinding unit are fixed; a cover body is slidably disposed at the opening, and the cover body is provided with a plurality of elastic elements corresponding to the perforations For pressing against the upper end of the test tube; at least one tapping rod, the striking rod is driven by a rotating motor to generate a tapping shock to the test tubes, and the knocking rod and the rotating motor are disposed at the Having a cavity, and the rotating motor comprises a rotating shaft provided with a horizontal turntable, the tapping bar is fixed on the horizontal turntable; and at least one variable speed control module is configured to control the rotating motor to be non-fixed The rotation speed drives the tapping rod to generate a non-fixed tapping frequency to the test tube, so as to drive the grinding unit to generate high and low frequency oscillations and perform cell lysis homogenization action on the sample to be tested. The cell lysis homogenization device according to claim 1, wherein the variable frequency speed control module can drive the rotation motor to generate a rotation speed change according to a predetermined input speed control program. The cell lysis homogenization device according to claim 1, wherein the polishing unit is at least one of a plurality of magnetic beads, iron beads, bead beads, grinding beads, and an abrasive. The cell lysis homogenization device according to claim 3, wherein the plurality of magnetic beads, iron beads, bead beads, and grinding beads are combined in different diameters. A cell lysis homogenization method comprising the steps of: a) preparing at least one test tube for accommodating a sample to be tested and at least one grinding unit; b) tapping the test tube at a non-fixed frequency; c) Using the grinding unit to generate high and low frequency oscillations during tapping, and performing cell lysis homogenization action on the sample to be tested. The cell lysis homogenization method according to claim 5, wherein in step b), the test tube is subjected to a non-fixed frequency tapping with at least one tapping rod, the striking rod is driven by a rotating motor, and At least one variable speed control module controls the rotating motor to generate a non-fixed rotational speed and produces a non-fixed frequency tap on the test tube. The cell cracking homogenization method according to claim 6, wherein the variable frequency speed control module drives the rotating motor to generate a rotation speed change according to a predetermined input speed control program.