TWI441655B - Composition containing piceatannol and method for preparing same - Google Patents

Description

Composition containing paclitaxel and method of producing the same

The present invention relates to a composition containing paclitaxel derived from paclitaxel derived from passion fruit seeds, and a method for producing the composition.

In the following Non-Patent Document 1, it is described that Resveratrol and Piceatannol which are derived from a polyphenol compound derived from grapes are caused by melanin-producing cells. The melanin production has an inhibitory effect. However, a commercially available reagent is used in the test for confirming the effect of the action, and a composition for obtaining a paclitaxel or the like containing a natural product such as grape is not described.

On the other hand, in the following Patent Document 1, it is described that resveratrol is concentrated and efficiently collected from the extract of the root of Reynoutria japonica Houtt. var. japnica, and is efficiently and efficiently collected. Further, in Patent Document 2 listed below, a Vaccinium vitis-idaea L extract containing resveratrol is loaded. Further, in the following Patent Documents 3 and 4, there is a description about the extract of Passion fruit (Passiflora sp.), especially the extract of the fruit of the passion fruit, and the use of the extract. Recorded in food, nutritional supplements, and medical use.

However, the techniques of the above documents do not provide a technique belonging to a composition obtained from a natural product and containing a high amount of paclitaxel.

[Advanced technical literature]

[Patent Literature]

[Patent Document 1] Japanese Patent Laid-Open Publication No. 2005-281179

[Patent Document 2] Japanese Patent Laid-Open Publication No. 2009-13159

[Patent Document 3] Japanese Patent Laid-Open Publication No. 2007-302659

[Patent Document 4] Japanese Patent Laid-Open Publication No. 2007-531734

[Non-patent literature]

[Non-Patent Document 1] Takako Yokozawa and You Jung Kim, Piceatannol Inhibits Melanogenesis By Its Antioxidative Actions, Biological & Pharmaceutical Bulletin Vol. 30 (2007) No. 11 p2007-2011

As far as the general concept of food safety is concerned, paclitaxel formulated in foods and drinks such as functional foods and health foods is preferably derived from natural products. However, in the past, natural products rich in paclitaxel were not known, and only compositions having extremely low contents were obtained from natural products such as grapes.

Accordingly, an object of the present invention is to provide a composition containing paclitaxel which is a composition derived from a natural product and which contains a high amount of paclitaxel, and a method for producing the composition containing the paclitaxel.

The present inventors have intensively studied in order to achieve the above object, and as a result, it has been found that the passion fruit seed which has been discarded in the past without being used for use is rich in paclitaxel, and thus the present invention has been completed.

That is, the composition containing paclitaxel of the present invention contains a content of from 0.0001 to 99.9% by mass of paclitaxel obtained from the passion fruit seed.

According to the composition containing paclitaxel according to the present invention, a high-content composition derived from natural substances containing paclitaxel can be provided. Then, since it is a composition derived from natural products, it is also suitable for blending into cosmetics and pharmaceuticals, and even foods such as functional foods and health foods.

On the other hand, in the method for producing a composition containing paclitaxel according to the present invention, at least one selected from the group consisting of a water-containing alcohol-based solvent and a water-containing ketone-based solvent is prepared by pulverizing the passion fruit seed. Stirring was carried out to extract the paclitaxel in the aforementioned solvent.

According to the method for producing a composition containing paclitaxel according to the present invention, since the aqueous alcohol-based solvent or the aqueous ketone-based solvent is used for extracting paclitaxel from the passion fruit seed, the yield can be favorably produced. It belongs to a composition derived from natural products and contains a high amount of paclitaxel.

In the method for producing a composition containing paclitaxel according to the present invention, it is preferred to pulverize the passion fruit seed, and then add any one of a water-based alcohol-based solvent and a water-containing ketone-based solvent, and stir. The first extract is obtained by extracting paclitaxel in a solvent, and further, in the residue which is not recovered as the extract, a water-containing alcohol-based solvent and a water-containing ketone-based solvent are added to the other unselected one and stirred. The second extract is obtained by extracting paclitaxel in the solvent, and mixing the first extract with the second extract. According to this aspect, since the stepwise extraction is carried out by a plurality of solvents, a high-content composition containing paclitaxel containing paclitaxel can be produced more preferably.

In the method for producing a composition containing paclitaxel according to the present invention, the seed of the passion fruit is preferably a seed of the passion fruit or a seed obtained by drying the seed of the passion fruit.

Further, in the method for producing a composition containing paclitaxel according to the present invention, the aqueous alcohol-based solvent is preferably aqueous ethanol, or the aqueous ketone-based solvent is preferably aqueous acetone. In terms of its water content, when it is aqueous ethanol, it is preferred to contain 20 to 99.9% by volume of ethanol. When it is aqueous acetone, it is preferred to contain 20 to 99.9% by volume of acetone. According to these aspects, the extraction efficiency of paclitaxel is better.

In the method for producing a composition containing paclitaxel according to the present invention, the stirring in each of the above solvents is preferably performed by ultrasonic vibration or vibration. According to this aspect, the extraction efficiency of paclitaxel is better.

Further, in the method for producing a composition containing paclitaxel according to the present invention, it is preferred to distill off the solvent under reduced pressure after the extraction.

On the other hand, another aspect of the present invention is a food or drink comprising adding a composition containing paclitaxel in an amount of 0.0001 to 99.9% by mass of paclitaxel obtained from passion fruit seeds.

Further, still another aspect of the present invention is a cosmetic comprising a composition containing paclitaxel containing 0.0001 to 99.9% by mass of paclitaxel obtained from passion fruit seeds.

According to the composition containing paclitaxel according to the present invention, a high-content composition derived from natural substances containing paclitaxel can be provided. Then, since it is a composition derived from natural products, it is also suitable for blending into cosmetics and pharmaceuticals, and even foods such as functional foods and health foods. On the other hand, according to the method for producing a composition containing paclitaxel according to the present invention, since an aqueous alcohol-based solvent or a water-containing ketone-based solvent is used as a solvent for extracting paclitaxel from the passion fruit seed, Therefore, it is possible to produce a composition which is derived from a natural product and which contains a high amount of paclitaxel in a good yield. Further, it is also possible to carry out stepwise extraction using a plurality of solvents which are aqueous alcohol-based solvents or aqueous ketone-based solvents, whereby it can be produced in a better yield.

The paclitaxel-containing composition of the present invention is a composition obtained from the passion fruit seed, and contains a content of 0.0001 to 99.9% by mass of paclitaxel. The shape may be a liquid shape, a solid shape, a semi-solid shape, a gel shape, or the like, or may be a passion fruit seed itself, or a slurry obtained by pulverizing seeds, or a powder obtained by freeze-drying and pulverizing seeds. Such as seed processing. The content of the solid content of the paclitaxel is preferably 0.0001 to 99.9% by mass, more preferably 0.01 to 50% by mass, and most preferably 0.1 to 10% by mass.

The paclitaxel is represented by the following chemical formula (1).

Although the paclitaxel represented by the above chemical formula is a trans-cetrubin having a structure in which a trans-alkenyl group is bonded to two phenyl groups, in the present invention, the concept of paclitaxel also includes the above chemical formula. The cis-alkenyl group is a cis-type paclitaxel having a structure in which two phenyl groups are bonded.

Passion fruit is a plant of the Passiflora genus Passiflora. It is also known as the fruit clock grass in Japan. Its fruit is eaten directly as a sweet and sour fruit, or its fruit juice is used as a fruit juice, or it is blended to Foods such as jelly and cakes are used for additional flavors. The seeds of passion fruit are hard. When they are made into juice, etc., the seeds are removed from the fruit. Even if the fruit is mixed with the seeds, it is directly excreted because the skin is hard and difficult to digest. The present invention is based on the discovery that paclitaxel is found to be rich in paclitaxel seeds which have been discarded in the past. Further, the passion fruit seed used in the present invention has been proven to have no problem in safety even if it is blended into functional foods such as functional foods and health foods.

The composition containing paclitaxel of the present invention is preferably obtained by, for example, pulverizing passion fruit seed or dried passion fruit seed, and extracting it using various solvents. In order to improve the extraction efficiency, the passion fruit seed may be appropriately subjected to chemical treatment such as acid or alkali decomposition or enzyme decomposition, and then extracted. Further, according to the method for producing a composition containing paclitaxel according to the present invention described below, since a composition containing a high amount of paclitaxel is particularly excellent in yield, it is obtained according to the method. optimal.

Hereinafter, a method for producing a composition containing paclitaxel according to the present invention will be described.

In the method for producing a composition containing paclitaxel according to the present invention, first, the passion fruit seed is pulverized. The passion fruit seed can directly use the raw seed or the dried passion fruit seed, and in order to make the extraction efficiency better, the seed of the passion fruit can be appropriately subjected to chemical treatment such as acid or alkali decomposition, enzyme decomposition, and the like. extraction. The pulverization can be carried out using a known pulverizer. Further, since the passion fruit seeds have a large amount of oil, they are in a slurry form after the pulverization.

Next, at least one selected from the group consisting of an aqueous alcohol-based solvent and a water-containing ketone-based solvent is added to the pulverized passion fruit seed and stirred, and the paclitaxel is extracted from the solvent.

As the aqueous alcohol-based solvent, an aqueous solvent such as ethanol, methanol or propanol can be used, and particularly preferably aqueous ethanol. Further, as the aqueous ketone solvent, an aqueous solvent such as acetone, methyl ethyl ketone, diethyl ketone or chloroacetone can be used, and in particular, aqueous acetone is preferred.

In terms of its water content, when it is aqueous ethanol, it is preferably from 20 to 99.9% by volume of ethanol, more preferably from 40 to 99.9% by volume, and most preferably from 60 to 80% by volume. good. When it is aqueous acetone, it is preferably from 20 to 99.9% by volume of acetone, more preferably from 40 to 99.9% by volume, and most preferably from 60 to 80% by volume. If it exceeds the above range, the extraction efficiency of paclitaxel may be deteriorated, which is not preferable. Further, in the following, for the purpose of simplifying the water content of the aqueous solvent, for example, 80% by volume of water containing 20% by volume of ethanol is referred to as "80% aqueous ethanol". Further, for example, 70% by volume of water containing 30% by volume of water is referred to as "70% aqueous ethanol". Further, for example, 30% by volume of 70% by volume of acetone containing water is referred to as "70% aqueous acetone".

In the method for producing a composition containing paclitaxel according to the present invention, it is preferred that the stirring in each of the above solvents be carried out by ultrasonic vibration or vibration. Thereby, since the vibration is effectively imparted to the passion fruit seed which is pulverized and contacted with each of the above solvents, the extraction efficiency of the paclitaxel from the passion fruit seed to the respective solvents can be improved. Ultrasonic vibration can be imparted by a well-known ultrasonic device. Also. The oscillation can be imparted by a well-known oscillating device.

Further, in the method for producing a composition containing paclitaxel according to the present invention, it is preferred to distill off the solvent under reduced pressure after the extraction. In this way, it is possible to obtain a composition containing no organic solvent, and it is suitable as a food material for use in foods and drinks such as functional foods and health foods, and is suitable for safety standards.

In the method for producing a composition containing paclitaxel according to the present invention, a plurality of kinds of solvents can be used for the stepwise extraction. Thereby, a composition containing paclitaxel containing a high amount of paclitaxel can be produced more preferably in a yield.

Specifically, for example, after the passion fruit seed is pulverized, any one of the aqueous alcohol-based solvent and the aqueous ketone-based solvent is added and stirred, and the first extraction is performed by extracting the paclitaxel in the solvent. Things. The extract is separated from the residue which is not recovered as the extract by centrifugation or the like, and the other one of the solvents selected is added to the residue and stirred, and the second extract is obtained by extracting the paclitaxel in the solvent. . Then, the first extract is mixed with the second extract. Further, of course, the second extract may be used alone as an extract of passion fruit seeds.

Further, it is considered that the passion fruit seed is subjected to the first extraction treatment by the aqueous alcohol-based solvent or the aqueous ketone-based solvent, and the properties such as texture are preferably improved by extraction. In the case where the second aqueous extraction treatment is carried out, it is not necessary to use the aqueous alcohol-based solvent or the aqueous ketone-based solvent, and it is expected that the extraction efficiency can be improved even if a solvent other than the solvent is used. Further, as shown in the examples to be described later, it is considered that the extraction efficiency of the aqueous alcohol-based solvent or the aqueous ketone-based solvent is preferably water-containing, so that the passion fruit seed is treated with water or an aqueous solvent. In the first treatment, it is needless to say that the aqueous alcohol-based solvent or the aqueous ketone-based solvent is used in the second extraction treatment, and it is expected that the extraction efficiency can be improved even if a solvent other than the solvent is used.

The extract of the passion fruit seed obtained by the above method may be directly or concentrated into a liquid form as a composition containing paclitaxel. Further, a powder which is pulverized by lyophilization or spray drying may be used as a composition containing paclitaxel. Moreover, it is not limited to such a form. The insoluble matter contained in the extract can be appropriately removed by filtration or the like. The insoluble matter may be further pulverized to obtain fine particles.

In addition, the primary extract derived from the passion fruit seed obtained by the above may also be subjected to ion exchange, size exclusion chromatography, HPLC method, gel filtration, membrane separation, etc., using paclitaxel as an index. It is differentiated and refined to make it a composition containing paclitaxel.

Specifically, for example, as shown in the examples below, when the composition extracted from the passion fruit seed is subjected to HPLC under the following conditions, the paclitaxel is eluted in the vicinity of 41 minutes. Further, when the mass spectrometry was performed by the mass spectrometer for this elution, the signal of the negative ion mode showed m/z 243.

[HPLC conditions]

‧Tube: Mightysil RP-18 GP150-4.6, diameter 4.6mm, length 150mm (made by Kanto Chemical Co., Ltd.)

‧column temperature: 40 ° C

‧ Dissolution conditions: flow rate 1mL / min, 0% methanol → 30% methanol (60 minutes)

‧UV detection: 280nm

Therefore, in the present invention, the physical properties of the paclitaxel as exemplified above are appropriately classified and refined, and the white cedar containing the seed of the passion fruit can be prepared by adjusting the content of the paclitaxel. The composition of the alcohol.

The composition containing paclitaxel of the present invention may be added with a pharmaceutically acceptable substrate or carrier as needed, and may be prepared into tablets, granules, powders, liquids, powders, granules, capsules, jelly (jelly The agent or the like is used in medicine, and may be used in cosmetics such as an ointment, a cream, a gel, a pack, a lotion, and a cosmetic.

Moreover, the composition containing the paclitaxel of the present invention can also be ingested in a specific health food, a nutritional supplement food, a functional food or the like. Such foods can be exemplified by chocolate, biscuit, chewing gum, candy, cookie, gummy, candy, etc.; cereal (cereal); powdered beverage, cool Beverages, dairy drinks, nutritional drinks, carbonated drinks, jelly-like drinks, etc.; ice cream, juice, sherbet and other freezing points. Further, preferred ones may also be exemplified by pastas such as Italian pasta, Udon noodles, and noodle. In addition, when it is used for a specific health food or nutraceutical food, it may be in the form of a powder, a granule, a capsule, a syrup, a lozenge, a sugar-coated tablet, or the like.

(Example)

The invention is specifically illustrated by the following examples, but these examples do not limit the scope of the invention.

<Test Example 1> [Detection ‧ Identification of paclitaxel derived from passion fruit]

The passion fruit, the pulp, and the seeds were separately lyophilized and pulverized by a pulverizer (product name "Sample mill type SK-M", Xie Li Technology Co., Ltd.), and then extracted with 80% aqueous ethanol. . After centrifugation, the supernatant was taken and concentrated by an evaporator, and then freeze-dried to obtain a powder. This powder was dissolved in water as an extract. Next, the solid concentration was diluted to 0.01 g/mL for the seed extract, and diluted to 0.1 g/mL for the peel extract or pulp extract, and then 5 μL of each was subjected to HPLC.

The analytical conditions of HPLC are as follows.

‧Tube: Mightysil RP-18 GP150-4.6, diameter 4.6mm, length 150mm (made by Kanto Chemical Co., Ltd.)

‧column temperature: 40 ° C

‧ Dissolution conditions: flow rate 1mL / min, 0% methanol → 30% methanol (60 minutes)

‧UV detection: 280nm

Fig. 1 (A) to Fig. 1 (C) show HPLC chromatograms of the above respective extracts. As a result, as shown in Fig. 1 (A), a peak was observed in the seed extract near 41 minutes. In contrast, the extract obtained from the peel and pulp had a solid concentration of 10 times that of the seed extract, and no peak was observed near 41 minutes.

Next, the substance in which the seed extract was dissolved in the vicinity of 41 minutes was identified by the following method.

First, the mass spectrum was measured using a KPLC/mass spectrometer (manufactured by Thermo Electro Co., Ltd., device name "Thermo-LCQ-MS/MS") (Fig. 2 (A)). In the positive ion mode and the negative ion mode 2 mode, it was found that good ions were observed in the negative ion mode, so the data was used. As a result, the main signal m/z 243 is (MH) ^- , the other peaks m/z 279 are attributed to (M+2H ₂ OH) ^- , and the m/z 487 is attributed to (M × 2-H) ^- . Therefore, the molecular weight was determined to be 244. Further, when the UV spectrum was obtained, λ _max was observed to be 220 nm, 304 nm, and 322 nm (Fig. 2(B)).

The results of these mass spectra and UV spectra are similar to those of polyphenols (Resveratrol, molecular weight 228) contained in peanut peel (see Fig. 3 (A), (B)). Further, it was confirmed that it was adsorbed to polyvinylpolypyrrolidone (PVPP: polyvinylpolypyrrolidone) which is a resin which adsorbs polyphenol. Further, the peak near the above 41 minutes has a smaller retention time of the reverse phase column than the retention time of resveratrol under the same HPLC conditions. As described above, the seed extract is eluted in the vicinity of the holding time of 41 minutes, and the molecular weight of one hydroxyl group is larger than that of resveratrol. Therefore, it is presumed to be paclitaxel represented by the following formula (1) ( Piceatannol, molecular weight 244).

Here, the pure compound of Taxolol (manufactured by SIGMA Co., Ltd.) was subjected to HPLC analysis under the same conditions as above to further obtain a mass spectrum and a UV spectrum (Fig. 4 (A), (B)). As a result, the retention time, mass spectrum, and UV spectrum in HPLC were completely consistent.

From the above, it was found that the peak of the above seed extract in the vicinity of 41 minutes of the above-mentioned HPLC analysis was the peak of paclitaxel, and it was revealed that the passion fruit seed contained a large amount of paclitaxel. On the other hand, paclitaxel could not be detected from the peel and pulp of passion fruit.

<Test Example 2> [Extraction of paclitaxel from passion fruit seeds 1]

Review the method for extracting paclitaxel from passion fruit seeds. Specifically, for the two types of passion fruit seeds produced in Japan and foreign countries, various solvents were used to investigate the extraction efficiency of paclitaxel.

(1) Extraction with 80% aqueous ethanol

50 mL of 80% aqueous ethanol was added to 5 g of the pulverized seeds, and extraction was performed while applying ultrasonic waves for 30 minutes. After the supernatant was recovered, the same operation was carried out twice, and a total of three extractions were performed. Then, the recovered extract was collected, and the obtained extract was adjusted to a volume of 200 mL with 80% aqueous ethanol, and then 10 μL of the mixture was subjected to HPLC. Then, the amount of the paclitaxel contained in the extract was quantified by comparing the area under the peak of the HPLC analysis with the standard curve of the standard concentration according to the usual method. Again, for reference, resveratrol is likewise dosed.

(2) Extracted with 70% aqueous acetone

The extraction residue after extraction with 80% aqueous ethanol was added, and 50 mL of 70% aqueous acetone was added thereto, and extraction was performed while applying ultrasonic waves for 30 minutes. After the supernatant was recovered, the same operation was carried out twice, and a total of three extractions were performed. Then, the recovered extract was collected, and the obtained extract was adjusted to a volume of 200 mL with 70% aqueous acetone, and the same operation as above was carried out to quantify the amount of the paclitaxel and resveratrol contained in the extract.

(3) Extraction with ethyl acetate

The solvent was replaced with ethyl acetate, and the seed of Passioni (foreign-made) was subjected to the same operation as the above-mentioned extraction with 80% aqueous ethanol, and the extracted paclitaxel and white squash extracted from 5 g of the freeze-dried seed powder were quantified. The amount of alcohol.

(4) Extracted with dichloromethane

The solvent was replaced by methylene chloride, and the seeds of passion fruit (foreign-made) were subjected to the same operation as the above-described extraction with 80% aqueous ethanol, and the amount of paclitaxel and chalk extracted from 5 g of the lyophilized seed powder was quantified. The amount of ruthenol.

The results are shown in Table 1. Further, in Table 1, the results of the extraction efficiency are expressed in terms of the amount of the extracted paclitaxel or resveratrol per 100 g of the freeze-dried seed powder.

As shown in Table 1, even the extraction residue after extraction with 80% aqueous ethanol extracted more paclitaxel than the extraction with 80% aqueous ethanol. On the other hand, the extraction efficiency when extracting only with ethyl acetate alone was not good, and when only extracted with dichloromethane alone, paclitaxel and resveratrol could not be detected. Therefore, it is known that extraction with 70% aqueous acetone is the most efficient. Further, it was found that the extraction efficiency was changed by using 80% aqueous ethanol extraction and extraction with 70% aqueous acetone.

<Test Example 3> [Extraction of paclitaxel from passion fruit seeds 2]

A further review of the method for extracting paclitaxel from passion fruit seeds. Specifically, it is investigated the influence of the water content of ethanol or acetone as a solvent on the extraction efficiency.

Therefore, ethanol or acetone having various water contents was used as a solvent, and the same as in Test Example 2 described above, and 5 g of the freeze-dried seed powder from the passion fruit (the above-mentioned foreign product) was used to extract the paclitaxel and resveratrol. The amount. Further, since the solvent ethyl acetate or methylene chloride used in the above Test Example 2 lacks compatibility with water, it is impossible to prepare a solvent having the same water content.

The results are shown in Table 2. Further, in Fig. 5, the results of paclitaxel are shown in the figure. Further, in Tables 2 and 5, the results of the extraction efficiency are also expressed in terms of the amount of the extracted paclitaxel or resveratrol per 100 g of the freeze-dried seed powder.

As shown in Table 2 and Figure 5, when ethanol is used, extraction with 80% aqueous ethanol is the most efficient. In addition, when acetone is used, extraction with 70% aqueous acetone is most efficient.

<Test Example 4>

In the above Test Example 2, the extract obtained by removing the solvent (from the above-mentioned foreign-produced passion fruit) was weighed, and the solid content of the paclitaxel was calculated. The values of these are shown in Table 3.

As shown in Table 3, when extracted with 80% aqueous ethanol, the solid concentration of paclitaxel was 2.6 mg / 100 mg of extract (2.6% by mass). Further, when extracted with 70% aqueous acetone after extraction with 80% aqueous ethanol, it was 3.7 mg / 100 mg of extract (3.7% by mass). From this, it was found that more extract of paclitaxel (as shown in Test Examples 2 and 3) was obtained by extraction with 70% aqueous acetone, and at the same time, higher purity of paclitaxel was obtained.

Fig. 1 is a graph showing an HPLC chromatogram of extracts of seed (A), peel (B) and pulp (C).

Fig. 2 is a graph showing the mass spectrum (A) and the UV spectrum (B) of the components eluted in the vicinity of 41 minutes by HPLC analysis in Fig. 1(A).

Figure 3 is a graph showing the mass spectrum (A) and UV spectrum (B) of resveratrol (molecular weight 228).

Figure 4 is a graph showing the mass spectrum (A) and UV spectrum (B) of paclitaxan (molecular weight 244).

Figure 5 is a graph showing the effect of the water content of ethanol (A) or acetone (B) in the solvent on the extraction efficiency of paclitaxel.

Since the figure in this case is the test result data of the compound, it is not the representative figure of this case, so there is no designated representative figure in this case.

Claims (11)

A use of passion fruit seeds for producing a composition containing paclitaxel containing from 0.0001 to 99.9% by mass of paclitaxel obtained from passion fruit seeds. A method for producing a composition containing paclitaxel, which comprises pulverizing a passion fruit seed, adding at least one selected from the group consisting of an aqueous alcohol-based solvent and an aqueous ketone-based solvent, and stirring, and extracting in the solvent Paclitaxel. A method for producing a composition containing paclitaxel according to claim 2, wherein after the passion fruit seed is pulverized, any one of an aqueous alcohol-based solvent and an aqueous ketone-based solvent is added and stirred. The first extract is obtained by extracting paclitaxel in a solvent, and further, the other unselected one of the aqueous alcohol-based solvent and the aqueous ketone-based solvent is added to the residue which is not recovered as the extract, and stirred. The second extract is obtained by extracting paclitaxel in the solvent, and mixing the first extract with the second extract. The method for producing a composition containing paclitaxel according to the second or third aspect of the invention, wherein the passion fruit seed is a seed of passion fruit or a seed obtained by drying the seed of passion fruit. The method for producing a composition containing paclitaxel according to the second or third aspect of the invention, wherein the aqueous alcohol-based solvent is aqueous ethanol or the aqueous ketone-based solvent is aqueous acetone. The method for producing a composition containing paclitaxel according to claim 5, wherein the aqueous ethanol contains 20 to 99.9 Accumulated in ethanol. The method for producing a composition containing paclitaxel according to claim 5, wherein the aqueous acetone contains 20 to 99.9% by volume of acetone. A method for producing a composition containing paclitaxel according to the second or third aspect of the patent application, wherein the stirring in each of the solvents is performed by ultrasonic vibration or vibration. A method for producing a composition containing paclitaxel according to the second or third aspect of the patent application, wherein after the extraction, the solvent is distilled off under reduced pressure. A use of a composition containing paclitaxel in which the content of paclitaxel obtained from the passion fruit seed is 0.0001 to 99.9% by mass is used for the manufacture of a food or drink. A use of a composition containing paclitaxel in which the content of paclitaxel obtained from the passion fruit seed is 0.0001 to 99.9% by mass, which is used for the manufacture of a cosmetic.