TWI429751B - Method for using micro-organisms transformation alga carbohydrate to acquire biomass crude - Google Patents

Description

Method for obtaining raw crude oil by microbial transformation of algal saccharide

The invention relates to a method for obtaining raw crude oil by microbial transformation of algal saccharides, which utilizes the biological characteristics of predetermined bacteria to bio-convert the saccharides in the algae to produce raw crude oil to obtain raw crude oil. A source of manufacturing oil for energy.

With the development of the global economy and the rapid increase of population, the demand for oils such as edible oil, energy oil or diesel oil has also increased. The source of refined oil is mainly plant and animal fat, while energy oil or diesel is used. It must be obtained through mining, but due to the high demand, the current dependence on plants and animal fats can no longer meet the needs of human beings. In addition, because the global ecological species are threatened, the environment caused by animal breeding will deteriorate more rapidly or It is not a viable solution to hunt wild animals and threaten the survival of wild animals. That is to say, the stock of energy oil mines around the world is also sending out warnings that will be exhausted, so how to save energy and how to seek new ones. Pipeline to obtain energy has become the most important area for human research.

As far as we know, there are quite a few technical departments that aim to obtain oils and fats in algae, and because algae are rich in protein, fat, algin and cellulose, polysaccharides and even trace elements and minerals, ie, microalgae It has photosynthetic organs such as chlorophyll, which can effectively convert H2O, CO2 and inorganic salts into organic compounds through photosynthesis. Therefore, the microalgae can absorb CO2 to slow down the greenhouse effect, while the microalgae breeds in two divisions. Mainly based on a short cell cycle and easy to carry out large-scale cultivation, so the required substances and energy are obtained in the algae body. It has gradually developed into an important research field of biotechnology.

In general, the algae has a very low oil content, which is only slightly less than 10%, and even only 2% to 3%. However, in the process of algae cultivation, algae are currently used to reduce nitrogen or also through the medium. The oil contained in the body is increased, so that the algae after the cultivation can provide more oil, but therefore, the overall cultivation and the subsequent action and the extraction process are delayed, but the efficiency is lowered, and the procedure is increased. The cost is thus increased.

In view of the above-mentioned shortcomings, the inventor believes that it is necessary to correct it, and that it has been engaged in the design and manufacture of related products for many years, and has always adhered to the excellent design concept, and researched and created the above disadvantages. After the effort, the present invention finally introduced a method for obtaining biomass crude oil by microbial conversion of algal saccharides, which is to improve the technology of the industry by correcting the excellent technology.

The invention adopts a method for microbial conversion of algal saccharides to obtain raw crude oil, which mainly utilizes the biological characteristics of the predetermined bacteria to effectively convert the substances in the algae into oils and fats, such as decomposing cellulose and generating short-chain fatty acids, thereby improving production. The overall efficiency of the oil process is the creative idea to solve the current crisis of rapid depletion of the Earth's energy.

In order to meet the purpose of the prior art, the method for obtaining biomass crude oil by microbial transformation of algae saccharide comprises the steps of: preparation of algae, addition of bacteria, biotransformation, product acquisition, etc., in the process of algae preparation In this way, the cultivation process that generally increases the oil contained in the algae by reducing nitrogen or also passing through the medium is omitted, because this method can obtain a larger amount of oil in the algae, but the working time is relatively better. Long, and only in the implementation process to reduce the occurrence of variables can achieve the highest practical effect, a more detailed description is that the more formalities, the more opportunities for failure (ie variables), and the invention after algae preparation Directly through the natural conversion between algae and bacteria organisms to produce crude oil, and about 20 to 50% of the algae body is cellulose, algae and polysaccharides, so in its substance The converted raw crude oil quantity can effectively achieve higher oil quantity than the prior art, which improves the efficiency of the overall process, and the raw crude oil also provides the energy oil required by humans, thereby achieving environmental protection. Energy efficiency and other benefits, and in line with industrial use.

1‧‧‧ algae preparation

2‧‧‧Adding bacteria

3‧‧‧Bioconversion

4‧‧‧Products

5‧‧‧Concentrated algae

6‧‧‧ Concentrated cells

First: Schematic diagram of the process of obtaining algin crude oil by the action of algae and bacteria in the present invention

The second figure: the flow diagram of another algae and bacteria in the invention to obtain raw crude oil

In order to more clearly express the technical focus of the present invention and to achieve the technical means to be used, a more detailed description will be made in the following description:

The invention relates to a method for obtaining raw crude oil by microbial transformation of algal saccharides, [refer to the first figure] mainly comprising: preparation of algae (1), which is to carry out large-scale cultivation of predetermined algae, and at the time of implementation, moderately Placed in the reactor, the algae used in the present invention are mainly non-oily algae, and the algae continues to carry out the subsequent action procedure in the reactor; the added bacteria (2), the equal amount of algae In the reactor, a predetermined strain is added, and the ratio between the bacteria and the algae is a ratio of 1:3, and the ambient temperature is set to be between 30 and 37 degrees, and the aqueous phase reacts. The aqueous phase reaction is a reaction between the bacteria and the algae, which treats the algae as a nutrient source, thereby secreting hydrolyzed enzymes to decompose the algal cell wall, and promoting the release of sugar and oil components in the algae. The reaction time is between 3 and 7 days, and the strain has the characteristics of decomposing sugars and producing oil and fat. The strains can use yeast or filamentous fungi, and the yeasts used can be: Saccharomyces Cerevisiae (Cryptococcus albidus) , filamentous fungus yeast (Lipomyces starkeyi), Trichosporon pullulans, oil-producing oil (Lipomyces lipofer), etc., the use of filamentous fungi can be: Aspergillus ter-rel28 (Aspergillus ter-rel28) , Cla-dosprium.fulvum, Entomphl-hora Coyonata), Helicostylum phrifome, Mucor javanicus, etc., and the strains are in the reactor and can interact with algae; biotransformation (3), in predetermined cells After entering the reactor and contacting with the algae, the predetermined cells secrete fibrinolytic enzymes, amylolytic enzymes, fat synthase, etc., and the various enzymes, for example, fatty acid synthase, ethylene coenzyme A carboxylate Acetyl CoA carboxylase, stearoyl CoA desaturase-1, and glutaric acid coenzyme A (HMGCoA ynthase) and glutaric acid coenzyme A ring The pro-enzyme (HMGCoA reductase) and the like are positively regulated, and the substance such as cellulose, non-starch polysaccharide, algin glue, etc. in the algae is converted into a saccharide, and the saccharide is subsequently produced by the bacterium through the fat synthase. Biomass crude oil; product (4) is obtained, that is, the raw crude oil produced is taken out, and the raw crude oil can be further converted into biodiesel by transesterification.

The invention adopts a method for microbial conversion of algal sugar to obtain raw crude oil, [refer to the first figure]. Simply put, after preparing the algae (1), the predetermined algae is placed in the reactor in an appropriate amount, and it is assumed that Under normal bioreactor operation, the amount of algae put in a single time is 15-30g / L, and then the effect of adding bacteria (2) in the reactor, due to the ratio of the amount of bacteria added to the algae is 1 :3, the relative amount of the cells is 5-10 g / L, and the amount of the algae is 15 g / L, and the amount of the bacteria is 5 g / L, let Biotransformation between the cells and the algae (3), the conversion is due to the secretion of fibrinolytic enzymes, amylolytic enzymes, etc., to the cellulose, non-amyloid polysaccharides in the algae The substance such as algin glue is converted into a saccharide body, and the saccharide body is used to produce a raw material crude oil through the fat synthase, whereby the produced raw crude oil can be taken out and used.

In addition, because the precursor of fat contained in algae is only about 10% of the whole, For a single incorporation of algae of 15g / L, and the amount of bacteria placed in 5g / L, the bacteria actually only work 10% of the total amount of algae (including the following), that is, bacteria In fact, the body can still act on the precursor of 90% (including above) algae fat, whereby the amount of algae can be placed up to 150g / L, but of course, 150g / L of algae can not be placed at one time It must be placed in stages to avoid the occurrence of matrix inhibition, which can achieve the best effect between the bacteria and algae more thoroughly.

In addition, algae and bacteria can be separately concentrated, [see the second figure]. After the algae is prepared (1), the algae (4) will be concentrated, and then the predetermined algae will be placed in the reactor in an appropriate amount. Since the algae has been concentrated, the amount of algae placed therein can reach 150g/L, and then the cells are concentrated (5), and the cells are added to the reactor (2). The ratio between the system and the algae is 1:3, so that the amount of the concentrated cells is 50 g/L, and the bio-conversion between the cells and the algae is performed (3). The conversion function is because the bacteria secrete fibrinolytic enzymes, amylolytic enzymes, etc., and the substances in the algae, such as cellulose, non-starch polysaccharides, algin glue, etc., are converted into sugar bodies, and the sugar bodies are subsequently supplied with bacteria. The body produces raw crude oil through fat synthase, etc., whereby the produced crude oil can be taken out for use, and the precursor of the fat contained in the algae is only about 150% of the whole body, so the algae The amount of insertion can be increased to 1500g/L, which must also be divided. Into segments to prevent a phenomenon inhibited by mass, whereby the reactor in an amount in terms of a single acting, are considerable.

Because in the whole oil extraction process, the algae does not need to change the biological composition of the algae individual through the medium, environment and the like, for example, by reducing nitrogen or permeating the medium to increase the oil contained in the algae, so The overall oil extraction process is reduced by at least one or more procedures. In addition, it acts as a natural conversion between organisms, and about 20-50% of the algae is cellulose, algin, and polysaccharide. Non-oily substances, so the amount of raw crude oil after conversion of the substance can be effectively higher than the prior art to increase the amount of oil, so the whole The process is more effective in improving the overall efficiency of production, and at the same time it provides the raw material crude oil that humans demand. This not only conforms to the concept of environmental protection, but also helps to delay the exploitation of earth resources by humans to effectively continue the survival of the earth and human beings.

In summary, it is understood that the present invention is novel, and the present invention is not found in any publication, and is in compliance with the provisions of Articles 21 and 22 of the Patent Law.

The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto. That is, the equivalent changes and modifications made by the invention in accordance with the scope of the invention are still within the scope of the invention.

1‧‧‧ algae preparation

2‧‧‧Adding bacteria

3‧‧‧Bioconversion

4‧‧‧Products

Claims (5)

A method for obtaining biomass crude oil by microbial conversion of algal saccharide, the method comprising the steps of: preparing algae by cultivating predetermined algae in a large scale, and placing it in a reactor to carry out production of raw crude oil; The product, that is, the produced raw crude oil is taken out; the improvement is: adding the acting bacteria, after the preparation of the algae, adding the predetermined strain to the reactor in which the algae is placed, and between the bacteria and the algae The ratio is a ratio of 1:3, and the ambient temperature is set between 30 and 37 degrees. The aqueous phase reacts, and the strains have the characteristics of decomposing sugars and producing oil; bioconversion The effect is that after the cells enter the reactor and contact with the algae, the cells secrete fibrinolytic enzymes, amylolytic enzymes, fat synthase, and substances in the algae such as cellulose, non-starch polysaccharides, algin glue, etc. It is converted into a saccharide body, which is followed by the production of raw crude oil by the bacteria through the fat synthase. The method for obtaining a raw crude oil by microbial conversion of algal saccharide according to the first aspect of the patent application, wherein the algae used in the preparation of the algae is preferably the non-oily algae. A method for obtaining a raw crude oil by microbial conversion of algal saccharides according to the first aspect of the invention, wherein the bacterial strain added to the reactor may be a yeast or a filamentous fungus. The method for obtaining a crude oil by microbial conversion of algal sugar according to the third aspect of the patent application, the strain of the yeast may be Cryptococcus albidus or Lipomyces starkeyi. , Trichosporon pullulans, Lipomyces lipofer Wait. The method for obtaining a crude oil by microbial conversion of algal saccharides according to the third aspect of the patent application, the strain of the filamentous fungus may be Aspergillus ter-rel28 or C. fuliginea (Cla-). Dosprium.fulvum), Entomphl-hora coyonata, Helicostyl phrifome, Mucor javanicus, and the like.