TWI418625B - Experimental system of widely used light genetic cells - Google Patents

Description

Extensive optogenetic cell assay system

The present invention relates to a culture device, and more particularly to a widely used optogenetic cell assay system for cultivating algae.

Optogenetic technology is mainly to make nerve cells become light-sensitive by means of gene transfer, thereby exploring specific neural circuits and contributing to the study of neuropsychiatric diseases.

For example, the light-sensitive protein (Channelrhodopsin-2; ChR2) in Chlamydomonas reinhardtii and the halophilic rhodopsin (NpHR) in the halophilic bacteria (Natronomonas pharaonis), the nerve cells can be exposed to light. After activation, an action potential is generated and the electrophysiological activity in the neurons can be controlled by light.

See Figure 1 for the current world leading Imperial (N. Grossman, V. Poher, MS Grubb, GT Kennedy, K. Nikolic, B. McGovern, RBPalmini, Z. Gong, EMDrakakis, MAANeil, MD Dawson, J .Burrone and P.Degenaar, Multi-site optical excitation using ChR2 and micro-LED Array, J. Neural Coll.7 2010,016004) The optogenetic cell experimental system developed by the team is mainly based on a light array 1 The light of the nerve cells is stimulated, whereby a scanning light wall can be formed to cause the light spots to act on the nerve cells in relative positions.

However, the light array 1 uses a large number of light-emitting diodes 11 as a light source, which not only has the problem of heat dissipation, but also increases the distribution area of the light spots. However, it is not necessary to enable the laser-receiving area of the nerve cells to fall accurately on the corresponding spot, which is likely to fall in the vicinity of adjacent spots, and affect the photo-excitation effect.

Therefore, the main object of the present invention is to provide a widely used optogenetic cell experimental system which can improve the accuracy of light spot and the effect of light excitation.

Therefore, the widely used optogenetic cell experimental system of the present invention is used for illuminating a photosynthetic organism, and comprises a stage, a light projection controller, and a central control unit. This stage is used to mount biological cells. The light projection controller projects a spot of light relative to cells on the stage. The central control unit is electrically connected to the light projection controller, and controls the light projection controller to generate an irregularly distributed number of light spots relative to the cells on the stage according to the required position, so that the light spots are projected on the cells on.

The effect of the invention is that the projection method can be used to accurately project the light spot at a desired position, thereby improving the spot accuracy and the light excitation effect.

The above and other technical contents, features and advantages of the present invention will be apparent from the following detailed description of the preferred embodiments.

Referring to FIG. 2, FIG. 3, and FIG. 4, a preferred embodiment of the widely used optogenetic cell experiment system of the present invention comprises a stage 2, a light projection controller 3, an image receiving module 4, and an optical magnification. Module 5, and a central control unit 6.

This stage 2 is used to mount biological cells.

The light projection controller 3 is disposed on the side of the stage 2, and has a light-emitting module 31 for generating a light source, a filter module 32 for separating the color light, and a chip module 33 for controlling the opening and closing of the light path. And an optical module 34 that projects the light spot. The light emitting module 31 has at least one light emitting diode 311. The filter module 32 is configured to separate red, blue, and green light in the optical path.

The image receiving module 4 is disposed above the stage 2 for receiving photons to generate images. In the preferred embodiment, the image receiving module 4 is a photosensitive coupling element (CCD).

The optical amplifying module 5 has a plurality of optical elements 51 that transmit light and are disposed between the image receiving module 4 and the light projection controller 3.

The central control unit 6 is electrically connected to the light projection controller 3, and controls the light projection controller 3 to project a plurality of light spots P according to the required position. In the preferred embodiment, the central control unit 6 is a computer in the preferred embodiment, and has a host 61, a screen 62, and an input device 63. The host 61 is used to process and transmit signals. The screen 62 is used to display a picture, and a light spot P' on the screen. The input device 63 has a mouse 631 and a keyboard 632 in the preferred embodiment for inputting the light spot P' and controlling The position of the spot P'.

During the experiment, the optical amplification module 5 and the image receiving module 4 can be enlarged in advance, and the image of the nerve cells can be acquired and displayed on the screen 62 of the central control unit 6. Then, only the screen 62 is needed to cooperate with the nerve The distribution position of the cells is input to the light spot P' on the screen displayed on the screen 62 by the input device 63 of the central control unit 6, and the position and color of the light spot P' are controlled, and the host 61 outputs a signal to the light. Projection controller 3, at this time, the light projection The light-emitting module 31 of the controller 3 generates a light source to form an optical path toward the optical module 34, and the filter module 32 separates red, blue, and green light in the optical path. At this time, the chip module 33 controls the light path to be turned on and off according to the position and color of the control spot P'. Finally, the light is concentrated by the optical module 34, and an irregularly distributed number of spots P are generated with respect to the cells on the stage 2 (see Fig. 5), the light spots P are projected at predetermined positions of the aforementioned cells. Thereby, not only can the nerve cells be activated by light, but also the electrophysiological activity in the neurons can be controlled by light.

As shown in FIG. 5, in addition to being able to cooperate with nerve cells to control the distribution position of the light spot P, the present invention can also control the spectrum of each light spot P, thereby giving color light of different characteristic bands to different nerve cells. The electrophysiological activity in neurons is maximized.

It can be seen from the above that the widely used optogenetic cell experimental system of the present invention has the following advantages and effects: the present invention can use the projection method to make the light spot P accurately projected at a desired level by using a single light source. In position, not only can the number of light sources be reduced, unnecessary light-emitting areas can be reduced, the cost can be reduced and the heat source can be reduced, and, importantly, the spot accuracy and the light excitation effect can be improved, and the invention is more practical and practical. Convenience.

The above is only the preferred embodiment of the present invention, and the scope of the invention is not limited thereto, that is, the simple equivalent changes and modifications made by the scope of the invention and the description of the invention are All remain within the scope of the invention patent.

1‧‧‧Light Array

11‧‧‧Lighting diode

2‧‧‧ stage

3‧‧‧Light Projection Controller

31‧‧‧Lighting module

311‧‧‧Lighting diode

32‧‧‧Filter Module

321‧‧‧Splitter

33‧‧‧ chip module

34‧‧‧Optical module

4‧‧‧Image receiving module

5‧‧‧Optical Amplifier Module

51‧‧‧Optical components

6‧‧‧Central Control Unit

61‧‧‧Host

62‧‧‧ screen

63‧‧‧ Input device

631‧‧‧ Mouse

632‧‧‧ keyboard

P, P’‧‧‧ light spots

1 is a cross-sectional view showing an optical array type optogenetic cell experimental system developed by the Imperial team; FIG. 2 is a front view showing a preferred embodiment of a widely used optogenetic cell experimental system of the present invention; A block diagram of the preferred embodiment; FIG. 4 is a schematic diagram of a central control unit in the preferred embodiment; and FIG. 5 is a cell organization diagram in which the light spot is red in the preferred embodiment.

3‧‧‧Light Projection Controller

4‧‧‧Image receiving module

5‧‧‧Optical Amplifier Module

6‧‧‧Central Control Unit

Claims (4)

A widely used optogenetic cell experimental system for illuminating photosynthesis organisms, the widely used optogenetic cell experimental system comprises: a loading platform for loading biological cells; a light projection controller disposed at the a side of the stage, and projecting a light spot relative to the cells on the stage; an image receiving module disposed above the stage for receiving photons to generate images; an optical amplifying module for transmitting light and having a digital optical component disposed between the image receiving module and the light projection controller; and a central control unit electrically connected to the light projection controller and the image receiving module, and having a host and a display for processing and transmitting signals a screen of the image, and an input device for inputting and controlling the position of the light spot, the host controls the light projection controller to generate an irregularly distributed number according to the required position according to the input position of the light, and the cell on the stage Light spots that project the spots onto the aforementioned cells. The widely used optogenetic cell experiment system according to the first aspect of the patent application, wherein the light projection controller has a light-emitting module for generating a light source, a filter module for separating color light, and for controlling light path opening, A chip module that is turned off, and an optical module that projects a light spot. The illuminating module according to claim 2, wherein the illuminating module has at least one illuminating diode. The widely used optogenetic cell experimental system according to the second application of the patent application, wherein the filter module is used for separating red light and blue light in the optical path. Light, green light.