1344377 九、發明說明： 【發明所屬之技術領域】 • 本發明係相關於一種纖維載體及其製法，尤指一種可 ·- 棋帶營養物質及具有緩釋效果的纖維載體及其製法。 【先前技術】 人體對營養物質的吸收量會就該物質在.飲食中的濃度 而有所影響，以單一劑量方式大量的攝取營養物質，其於 雜内之吸收效果會低於多次低劑量的攝取方式，也因此具 • 有緩釋效果的食物配方是提高某營養物質在體内吸收量的 常用方法。緩釋製劑被廣泛應用於不同的藥物和營養物質 中，方式之一是採用一些高分子的合成材料作載體，當中 加入藥物或營養物質，攝入製劑後緩慢釋放出當中的藥物 或營養物質’藉此提高這些物質的體内吸收。 - 纖維為日常飲食的不可或缺的一部份’並具有生理活 性功能’在人體消化道中不被消化吸收，每天建議攝取量 一般為25-30克，學術文獻指出纖維本身對报多物質具有 • 吸附能力，能將部份物質排除到體外，而有關利用天然纖 維作為緩釋配方之載體，提高營養物質吸收的研究文獻頗 少。因此，有鑒於利用纖維作為載體的技術與產品鮮少， 而有待進一步提供先進而實用之技術與相關產品。 【發明内容】 有鑑於利用纖維素材作為載體的技術與纖維載體之不 足’同時製備維生素緩釋配方有助天然保健食材之開發， 本發明主要目的在於利用纖維素材作為材料，提供一種具 1344377 有攜帶及緩釋維生素功效的纖維載體-維生素複合物之製備 方法，可提向纖維攜帶維生素之能力，並有緩慢釋放維生 • 素之效果，攝食後可幫助維持灰漿維生素有較高濃度從以 . 幫助吸收。 . 為達成以上的目的，本發明係提供一種纖維載體的製 法，其係包含： 提供纖維素材； 使纖維素材經研磨處理；及 籲 獲取粒徑在1〇 mm至〇.〇1 pm的纖維粉末。 較佳的是，纖維素材為天然纖維或無毒性的人造纖 維，更佳的是，該無毒性的人造纖維為羧甲基纖維素或羧 甲基幾丁聚醣》 較佳的疋’天然纖維係由蔬果經榨汁後所獲取；更佳 ' 的是，天然纖維係經蔬果榨汁並加以乾燥後所獲取。 較佳的是’乾燥條件為⑽它至35〇c乾燥24至96小 時’更佳的是’乾燥條件係為“乞至45〇c乾燥24至72 鲁小時；最佳的是，乾燥條件係為48。(：乾燥48小時。 較佳的是’蔬果係為富含纖維質的蔬果；更佳的是， 畜含纖維質的蔬果為菇蕈類、蔬菜類或水果類。 在本發明的一具體實施例，蔬菜類為十字花科 iBrassica)蔬茱、議 i 料（Cucurt>j taceae)蔬茱、旋庀料 iConv〇Ivujacea幻扳杗、％^(Ajnaranthaceae)扳茱、緣 科 iChenopodiaceae')氣 1、禾本科 QGramjneae、氣 i、天 南星科（蔬菜、百合科蔬菜、錦葵科 1344377 蔬菜、茄科蔬菜、繳形花科 (Umbel li ferae)蔬菜或薑科（//；^/kraCeae)。 在本發明的一較佳具體實施例，十字花科蔬菜為白 菜、高麗菜、芥蘭菜、青江菜、綠花椰菜或花椰菜；在本 發明的另一較佳具體實施例，葫蘆科蔬菜為絲瓜、小黃瓜、 胡瓜、甜瓜、冬瓜或南瓜；在本發明的另一較佳具體實施 例’缴形化科蔬菜為芽菜或胡蘿蔔。 在本發明的一具體實施例’水果類為楊桃、菠蘿、香 蕉、番荔枝、木瓜、芒果、番石榴、蓮霧、檳榔、橘、柚、 棗、揶子、枇杷、梅或李。 較佳的是，所獲取的纖維粉末粒徑為〇 5 mm至】〇 mm ;更佳的是，所獲取的纖維粉末粒徑為〇 ι丨〇 。 本發明另相關於一種纖維載體，其係經由申請專利範 圍第1至14項任一項所製得。 較佳的是，該纖維載體所載之活性物質為維生素藥 物或脂溶性營養品：更佳的是，該纖維載體所載之藥物為 輔瞒Q10、茶驗或既有需使用載體的藥物種類，例如氛茶 鹼克拉黴素、順氣麵、羅紅黴素、阿司匹林或丙戍酸錢。 本發明另相關於-種具有緩釋效果的載劑，其包含係 =申請專利範4項任—項所製得的纖維栽 較佳的是’該纖維載體所載之活性物質為維生素 物或脂溶性普表σ .宙乂ΑΒ 、 8養。°，更佳的是，該纖維載體所載之藥物為 1〇、茶驗或既有可以緩釋劑型態使用㈣物，例如 1344377 抗疼痛藥、抗癌藥、抗病毒藥、抗真菌藥、類固醇藥物、 非固醇藥物、抗發炎劑、抗過敏劑、抗細胞增生劑等。 藉此’本發明所達成的具體功效包括： 1 ·本發明係利用纖維素材（包含天然纖維及人造纖維） 作為載體’與維生素、食用油及水進行混合與均質，製備 成纖維•維生素複合物。可有效提高纖維攜帶維生素之能 力。 2 .本發明可使纖維表面結構改變，暴露出較多的官 能基’可有效應用在纖維之官能基修飾。 3 .本發明所製備的纖維_維生素複合物具有緩慢釋放 維生素之優點。 4 .本發明所製備的纖維-維生素複合物經攝食後，於 體内可幫助維持血毁中維生素E於至少5小時内有較高的 濃度，幫助維生素之吸收。 【實施方式】 本發明是利用纖維素材（較佳的是為純天然食材所獲 2天然:纖維），透過快速、簡單和低成本的方式，開發 物：：營養物質具有攜帶和緩釋能力的營養品或藥品等 =將來使用在保健食材之中’幫助提高營養之吸收。 =明可進__步作相發纖維素材作為緩釋配方之載 素”明之纖維載體不僅展現可提高纖維本身攜帶維生 持血漿中維生棄攝食後可幫助維 收。未杳；、内有較高的濃度，從以幫助吸 收未來可使用日常飲食的篇助及 『次缺的食用纖維，開發成 1344377 為營養物質的載體和緩釋配方’幫助營養物質的體内吸 收，有助新保健素材或保健食品之開發。 在本發明所指的『纖維素材』意指天然纖維或無毒性 / 的纖維（包含人造纖維）；無毒性的纖維，意指對人體或動 • 物體不會產生明顯毒害作用（例如°惡心、章眩、區吐、中 J毒等）的纖維，其中無毒性的人造纖維可為羧甲基纖維素 或羧甲基幾丁聚醣或其類似物。 在本發明所指的『天然纖維』或『無毒人造纖維』意 _ 心不能被動物的消化酵素所水解的多醣類，在本發明中尤 才曰植物性食物中不能被人類的消化酵素所水解的多醣類， 該纖維對不具動物（包括人類）毒性或傷害。 在本發明所指的『載體』意指可攜帶活性物質的揭帶 物質，在本發明中尤指該經研磨後的天然纖維或無毒；『載 • 劑』意指包含本發明經研磨後的天然纖維及其他賦型劑、 黏著劑、緩釋劑、色素等物質後的劑型》 在本發明所指的『緩慢釋放』或『緩釋』或『緩釋配 _ 方』意指在一定時間内，以穩定速率逐漸釋放活性物質的 狀態或配方。緩慢釋放或緩釋狀態係可活性物質，在受試 體（如動物體、人類）的體内或血液内維持一定濃度，以 避免突然提高濃度所造成的不適。 在本發明中所使用的統計分析方法及實驗數據表示方 法說明如下：實驗數據以平均質土標準偏差值表示（樣品 數為4 ’體内試驗樣品數為6) ’並以Statistical Analysis1344377 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a fibrous carrier and a method for producing the same, and more particularly to a fibrous carrier capable of having a nutrient and a sustained release effect, and a method for preparing the same. [Prior Art] The amount of nutrients absorbed by the human body will be affected by the concentration of the substance in the diet. A large amount of nutrients will be taken in a single dose, and the absorption effect in the impurities will be lower than that of multiple low doses. The method of ingestion, and therefore the food formulation with sustained release effect, is a common method to increase the absorption of a nutrient in the body. Sustained-release preparations are widely used in different medicines and nutrients. One of the ways is to use some synthetic materials of polymers as carriers, adding drugs or nutrients, and slowly releasing the drugs or nutrients after ingesting the preparations. Thereby increasing the absorption of these substances in the body. - Fiber is an indispensable part of the daily diet 'and has physiologically active functions' that are not digested and absorbed in the human digestive tract. The recommended daily intake is generally 25-30 grams. The academic literature indicates that the fiber itself has multiple substances for reporting. • Adsorption capacity, which can exclude some substances from outside the body, and there are few research literatures on using natural fiber as a carrier for sustained-release formula to improve nutrient absorption. Therefore, in view of the fact that there are few technologies and products using fibers as carriers, it is necessary to further provide advanced and practical technologies and related products. SUMMARY OF THE INVENTION In view of the insufficiency of the technology and the fiber carrier using the fiber material as a carrier, the simultaneous preparation of the vitamin sustained-release formulation contributes to the development of the natural health food material. The main purpose of the present invention is to use the fiber material as a material to provide a carrier with 1344377 And the preparation method of the fiber carrier-vitamin complex with sustained-release vitamin function, can bring the ability of carrying vitamins to the fiber, and has the effect of slowly releasing the vitamin, and can help maintain the high concentration of the vitamin in the mortar after ingestion. Help absorb. In order to achieve the above object, the present invention provides a method for producing a fiber carrier, comprising: providing a fiber material; grinding the fiber material; and obtaining a fiber powder having a particle diameter of from 1 mm to 〇. . Preferably, the fiber material is natural fiber or non-toxic rayon fiber, and more preferably, the non-toxic rayon fiber is carboxymethyl cellulose or carboxymethyl chitosan. It is obtained by extracting fruits and vegetables from the juice; better yet, the natural fiber is obtained by juice and drying the fruits and vegetables. Preferably, the drying condition is (10) it is dried to 35 〇c for 24 to 96 hours. More preferably, the drying condition is "dry to 45 〇c drying for 24 to 72 hr hours; optimally, the drying conditions are 48. (: drying for 48 hours. Preferably, the 'fruit and vegetable is a fiber-rich vegetable and fruit; more preferably, the fiber-containing vegetable and fruit is mushroom, vegetable or fruit. In the invention In one embodiment, the vegetables are cruciferous iBrassica) vegetable, vegetable (Cucurt> j taceae) vegetable, rotary condiment iConv〇Ivujacea illusion, %^(Ajnaranthaceae), 缘科iChenopodiaceae' Gas 1, Gramineae QGramjneae, Qi i, Araceae (vegetables, Liliaceae vegetables, Malvaceae 1344377 vegetables, Solanaceae vegetables, Umbel li ferae vegetables or Zingiberaceae (//;^/kraCeae In a preferred embodiment of the present invention, the cruciferous vegetable is cabbage, Korean cabbage, kale, Qingjiang, broccoli or broccoli; in another preferred embodiment of the present invention, cucurbitaceae vegetables For loofah, gherkin, cucurbit, melon, melon or pumpkin; Another preferred embodiment of the invention is a sprouting vegetable or a carrot. In a specific embodiment of the invention, the fruits are carambola, pineapple, banana, sweetsop, papaya, mango, guava, lotus. Fog, betel nut, tangerine, pomelo, jujube, hazelnut, medlar, plum or plum. Preferably, the obtained fiber powder has a particle size of 〇5 mm to 〇mm; more preferably, the obtained fiber powder The particle size is 〇ι丨〇. The present invention is further related to a fiber carrier which is produced according to any one of claims 1 to 14. Preferably, the active substance contained in the fiber carrier is a vitamin. The drug or the fat-soluble nutrient: more preferably, the drug contained in the fiber carrier is an auxiliary drug Q10, a tea test or a drug type which requires a carrier, such as catechin clarithromycin, cis-gas surface, and rosacea. The present invention is further related to a carrier having a sustained release effect, which comprises a fiber plant prepared according to the application of the patent item 4, which is preferably a fiber. The active substance contained in the carrier is a vitamin or a fat Preferably, the drug contained in the fiber carrier is 1 〇, tea tested or used in a sustained release dosage form (4), such as 1344377 anti-pain drug , anticancer drugs, antiviral drugs, antifungal drugs, steroid drugs, nonsteroidal drugs, anti-inflammatory agents, anti-allergic agents, anti-cell proliferative agents, etc. The specific effects achieved by the present invention include: 1 · Ben The invention uses a fiber material (including natural fibers and rayon) as a carrier to mix and homogenize with vitamins, edible oil and water to prepare a fiber-vitamin complex. It can effectively increase the ability of fiber to carry vitamins. 2. The present invention allows the surface structure of the fiber to be altered, and the exposure of more functional groups can be effectively applied to the functional group modification of the fiber. 3. The fiber-vitamin complex prepared by the present invention has the advantage of slowly releasing vitamins. 4. After the fiber-vitamin complex prepared by the invention is ingested, it can help maintain the blood-destroy vitamin E at a higher concentration for at least 5 hours and help the absorption of the vitamin. [Embodiment] The present invention utilizes a fiber material (preferably 2 natural: fiber obtained from pure natural ingredients) in a fast, simple and low-cost manner, and the development: nutrient has the ability to carry and release. Nutrition or medicines, etc. = will be used in health foods in the future to help increase the absorption of nutrients. = Ming can enter __ step for the fiber material as a carrier of the sustained-release formula "Mingzhi's fiber carrier not only shows that the fiber itself can carry the vitamins in the plasma, and the vitamins can be absorbed in the plasma. There is a higher concentration, which helps the absorption of nutrients in the body by helping to absorb the daily diet that can be used in the daily diet and the "negative dietary fiber, developed into 1344377 as a carrier for nutrients and a sustained-release formula". Development of health care materials or health foods. The term "fibrous material" as used in the present invention means natural fibers or non-toxic/fibers (including rayon); non-toxic fibers, meaning that they are not produced on human bodies or moving objects. A fiber having an obvious toxic effect (for example, nausea, trichomoniasis, exudation, venom, etc.), wherein the non-toxic rayon may be carboxymethylcellulose or carboxymethyl chitosan or the like. The "natural fiber" or "non-toxic rayon" as referred to in the present invention means that the polysaccharide which cannot be hydrolyzed by the animal's digestive enzyme can not be digested by humans in the present invention. a polysaccharide which is hydrolyzed, which is toxic or harmful to animals (including humans). The term "carrier" as used in the present invention means a stripping substance capable of carrying an active substance, especially in the present invention. After the natural fiber or non-toxic; "loading agent" means the dosage form comprising the ground natural fiber and other excipients, adhesives, sustained release agents, pigments and the like according to the present invention. Slow release or "sustained release" or "sustained release" means a state or formulation in which the active substance is gradually released at a steady rate for a certain period of time. The slow release or sustained release state is an active substance in the subject. (In animal, human) maintain a certain concentration in the body or in the blood to avoid sudden increase in concentration. The statistical analysis methods and experimental data representation methods used in the present invention are as follows: Experimental data is averaged The soil standard deviation value indicates (the number of samples is 4 'the number of test samples in the body is 6)' and statistical analysis
System (SAS)軟體進行統計分析’尸< 0.05視為具顯著性 1344377 之差異。 實施例 實施例一：天然織維之製儇 在本發明中主要係以楊桃，作為天然纖維的來源。胃 桃的天然纖維製備方法，包括將楊桃釋汁後收集果渔將 果〉査於48。〇乾燥48小時後，將經乾燥的果逢加以研磨使 其成為粒徑為〇.5mm的粉末，再研磨此非水溶性纖維樣品 至平均粒徑為Ο.ΜΟμπ^^、。該粉末的渔度含量為Η 9 土 〇_7 g/kg (Chau et al)。The System (SAS) software was statistically analyzed for 'corporate' 0.05 as a significant difference of 1344377. EXAMPLES Example 1: Natural Weaving Processes In the present invention, carambola is mainly used as a source of natural fibers. The method for preparing natural fiber of stomach peach, including collecting the fruit of the carambola after the juice is released, is checked. After drying for 48 hours, the dried fruit was ground to a powder having a particle diameter of 0.5 mm, and the water-insoluble fiber sample was ground to an average particle diameter of Ο.ΜΟμπ^^. The fish has a fishmeal content of Η 9 〇 7 g/kg (Chau et al).
實施例一·製餚天然植物非水溶性織維維生素E 合物 天然植物纖維研磨至適當顆粒大小的粉末（〇 5職的 大小）。再研磨此非水溶性纖維樣品至平均粒徑為〇 i i〇pm 的大小，接者取1 g此研磨後的纖維粉末及5〇 mg的維生 素E，以0.5 ml的油混合，在加入適量的水，並以η嶋_ 1· psi壓力之高壓均質機進行均質(對照組略過此步 驟)，此混合物需均質至可保持12小時未分層的乳化狀態， 而後以3〇〇〇g離心20分鐘後凍乾，並收集未均質非水溶 性纖維-維生素混合物及均質化纖維-維生素複合物。 丨 實施例三：雄生素E釋放試驗 3.1體外試驗 1 g之纖維-維生素複合物加入100 m丨之人工胃液作為 溶解媒介，在37°C超音波連續震盪下，於兩小時内每 分鐘取樣1 m卜經過濾後直接以Ηριχ測定其中維生素e 1344377 的含量，複合物中維生素E之釋放率以時間及人工胃液中 所釋出之維生素E含量表示。 2體内試驗 12隻體重約250 ± 18克之雄性SD品系大鼠隨機分配 至兩組不同膳食組別，包括控制組及均質化纖維-維生素E 複合物組。於複合物組別之大鼠以管餵的方式投以含〇2 g 均質化纖維-維生素E複合物之1 mi玉米油（提供2.14 mg 之維生素E) ’而控制組則以管餵方式投以imi含維生素e (2.14 mg)之玉米油。經餵食不同組別的樣品後，於五小時 内母小時為間隔於大鼠尾部取血，血漿樣品（1 〇 〇 ) 及 50 μί 内標準品 Sigma T3376 α-tocopherol acetate (52.5 mg per liter 〇f ethan〇l)—同震盪混合一分鐘。混合物中維 生素Ε再以200 μι HPLC級己烷萃取並震盪混合一分鐘， 經670 g離心兩分鐘後，收.集己烷層並以氮氣吹乾，殘留 物以50 HPLC級甲醇溶解，並以HPLC進行維生素E 含量分析。複合物於體内維生素£之釋放率以一定時間内 大鼠血漿中維生素E濃度改變作計算β 結果 第一圖顯示一定時間内（0-2 h)未經均質之織維混合 物及均質化纖維-維生素複合物於人工胃液中維生素e之釋 放率。未經均質之纖維混合物在此時間内維生素E之釋激 量並無改變；而在此兩小時期間，均質化之纖維維生素潜 合物隨者時間增加而逐漸的累積所釋放之維生素e含量， 在120分鐘時，其維生素e的累積量達到85 2 ^^化。丑 11 1344377 結果證明了經均質之雄n ^ 之纖維-維生素複合物可在兩小時 且緩慢的釋放維生t E，此體外試驗並證實了均質化纖維_ 維生素複合物具有顯著之維生素E攜帶力並可 所攜帶之物質，間接提 ^ 帶活性物質之載體，光目士上上ώ 调 戰骽並具有相當良好之緩釋功效，可增加 其於食品上之應用；此具有緩釋作用之均質化纖維·維生素 複合物可延長營養物質於體内的釋放時間，進一步可增進 維生素Ε之體内吸收效率。Example 1 Manufacture of natural plant water-insoluble woven vitamin E compound The natural plant fiber was ground to a powder of a suitable particle size (the size of the 〇 5 job). Then grind the water-insoluble fiber sample to an average particle size of 〇 〇 〇 pm, and take 1 g of the ground fiber powder and 5 〇 mg of vitamin E, mixed with 0.5 ml of oil, and add an appropriate amount. The water was homogenized by a high pressure homogenizer with a pressure of η嶋_1·psi (the control group skipped this step), and the mixture was homogenized to an emulsified state which was maintained for 12 hours without delamination, and then centrifuged at 3 〇〇〇g. After 20 minutes, it was lyophilized and the heterogeneous water-insoluble fiber-vitamin mixture and homogenized fiber-vitamin complex were collected.丨Example 3: Androgen E release test 3.1 In vitro test 1 g of fiber-vitamin complex was added to the artificial gastric juice of 100 m 作为 as a dissolution medium, and samples were taken every minute for two hours under continuous shaking at 37 ° C. After filtering, the content of vitamin E 1344377 was directly measured by Ηριχ, and the release rate of vitamin E in the complex was expressed by time and the vitamin E content released from the artificial gastric juice. 2 In vivo test Twelve male SD strains weighing approximately 250 ± 18 g were randomly assigned to two different dietary groups, including the control group and the homogenized fiber-to-vitamin E complex group. Rats in the complex group were given a 1 g corn oil containing 2 g of homogenized fiber-vitamin E complex (providing 2.14 mg of vitamin E) by tube feeding, while the control group was administered by tube feeding. A corn oil containing vitamin E (2.14 mg). After feeding different groups of samples, the mothers were bled at the tail of the rats within 5 hours, plasma samples (1 〇〇) and 50 μί internal standard Sigma T3376 α-tocopherol acetate (52.5 mg per liter 〇f Ethan〇l) - Mix for one minute with the shock. The vitamin Ε in the mixture was extracted with 200 μM HPLC grade hexane and shaken for one minute. After centrifugation at 670 g for two minutes, the hexane layer was collected and dried with nitrogen, and the residue was dissolved in 50 HPLC grade methanol. HPLC analysis of vitamin E content. The release rate of vitamins in the complex is calculated by changing the concentration of vitamin E in the rat plasma for a certain period of time. The first graph shows that the homogenous mixture and homogenized fiber are not homogenized within a certain period of time (0-2 h). - The release rate of vitamin E in the artificial gastric juice of the vitamin complex. The release of vitamin E did not change during this time without homogenized fiber mixture; during this two-hour period, the homogenized fiber vitamin complex gradually accumulated and released the vitamin E content. At 120 minutes, the cumulative amount of vitamin E reached 85 2 ^^. Ugly 11 1344377 The results demonstrate that the homogenized male n ^ fiber-vitamin complex can release vitamin E at two hours and slowly, this in vitro test confirmed that the homogenized fiber _ vitamin complex has significant vitamin E carrying capacity And the substances that can be carried, indirectly, carry the carrier of the active substance, and the Guangshishi Shangyu has a good sustained release effect, which can increase its application in food; this has the homogenization of sustained release. The chemical fiber and vitamin complex can prolong the release time of nutrients in the body, and further improve the absorption efficiency of vitamins in the body.
表一顯示，控制組大鼠血漿_維生素Ε的濃度在第一 小時提升至其高峰值（約起始量< 3 8倍），接者於兩小 時内回復至正常值。於此實驗期間（1、2、3及4小時）， 7質化之纖維·維生素複合物可維持大鼠血漿維生素Ε濃度 刀另】達原本之2.1、3.1、3.6及2.2倍，並於第三小時達最 佳之濃度；與控制組比較，均質化之纖維維生素複合物之 緩釋作用可増加血漿維生素濃度至少達五小時，此體内實 驗再一次呼應了體外試驗的結果，均質化之纖維·維生素複 合物是相當有潛力之維生素Ε載體並具有緩慢釋放維生素 之功效，因此攝入此均質化之纖維·維生素複合物可增進維 生素Ε於體内之吸收。 1344377 表一、經攝食可食用植物纖維-維生素複合物後大鼠體 内血漿維生素E的改雙_Table 1 shows that the concentration of plasma _vitamin oxime in the control group was increased to its high peak (about the initial amount < 38 times) in the first hour, and the recipient returned to the normal value within two hours. During the experiment (1, 2, 3, and 4 hours), the 7-mass fiber-vitamin complex maintains the plasma concentration of vitamin Ε in rats, which is 2.1, 3.1, 3.6, and 2.2 times the original. The optimal concentration was obtained in three hours; compared with the control group, the sustained release of the homogenized fiber-vitamin complex could increase the plasma vitamin concentration for at least five hours. This in vivo experiment once again echoed the results of the in vitro test, homogenizing Fiber and vitamin complexes are quite promising vitamins and have a slow release of vitamins. Therefore, the ingestion of this homogenized fiber and vitamin complex can enhance the absorption of vitamins in the body. 1344377 Table 1. Changes in plasma vitamin E in rats after ingestion of edible plant fiber-vitamin complexes_
時間⑻ 對照組b 織維·維生素複合物 0 26.4 ± 2·5ν 24·7±2.4ν 1 100±6.7w 51.7±3.2w 2 37.8 ± 3.6x 76.9 ± 5·2χ 3 28.0±1.9v 88.7 ± 6.4y 4 26·7 ± 2.0v 55.4 ± 5_5w 5 26.4 士 1 ·6ν 37.7 ± 3.6z -if Γ攔中但標示不同字母的數值（平均值±標準偏差，η -6)，具有顯著差異者（/> < 〇,〇5)。 為2 1 4^?\組與纖維—維生素複合物試驗組所設取維生素Ε的總量 實施例四：維生素Ε攜帶能力之估算 ,根據方法“體外維生素Ε釋放試驗，，所描述的方法作些 微修改，不同複合物間維生素_帶能力（mg/g)以測定經 37 C超音波連續震盪6小時之人工胃液過濾液中維生素ε 的3量作為維生素E攜帶能力之估算。 結果 圃马禾經均 複合物之維生素E攜 生素複合物可攜帶相 混合物所攜帶之維生 生素複合物之維生素 (955¼) ’此結果顯示 複合物之維生素攜帶 頁之纖維混合物及均質化纖維·維生 帶能力分析，結果顯示均質化纖維_ ®大量的維生素E，未經均質之纖 素E為（M2 mg/g，而均質化纖維-丨 E攜帶能力則大幅提升至1〇7 出均質化處理可增加揚桃織維維生: 量至約9.6倍，其原因可能為經均； 13 1344377 化處理後之纖維表面暴露出更多的官能基如幾基、羧基及 羥基（第二圖）等’因此提升了維生素之攜帶力；此卓 越的維生f E攜帶能力提示了均f化處理之非水溶性纖 維’可以新額之纖維.維生素複合物的型式，作為具有相當 潛力脂溶性活性成分之載體。 實施例五：傅式轉換紅外線光譜（FTIR)分析 非水溶性纖維樣品（約丨g)與⑽mg之漠化鉀混合 後取一部分以167 MPa之壓力進行壓片，壓片後以4〇〇c 溫度下烘乾24小時後置於乾燥箱保存以待分析^ ftir分 析以 Bruker Equinox55 spectr_ter (BiUerica，usa)於約 500-4000·1波長範圍内進行分析。 結果 第二圖為均質化處理對非水溶性纖維之官能基影響分 析，以FTIR測試均質化處理對其表面官能基的影響。結 果顯示，與未經均質之非水溶性纖維之FTIR圖譜比較（第 三B圖），經均質後之非水溶性纖維於1655、丨的❶及Η” cm 1暴露出新的C=0吸收峰，並於35〇〇〜39〇〇⑽一暴露出 新的0-H吸收峰（第三A圖），此結果顯示出均質化處理 可使纖維表面結構改變，並暴露出更多的官能基如羰基、 羧基及羥基，因此均質化處理可應用在修飾楊桃非水溶性 纖維之官能基；在理化性質試驗中，均質化處理後之楊桃 非水溶性纖維展現出較高的保油力（約2〇 4倍），因此可 得知這些官能基的改變進一步影響了樣品的特性，更可改 善其理化性質，增加在食品方面的應用領域。 14 1344377 π 〇上述實施例與結果顯示，證明了經均質之纖維-維 素複σ物可開發作為具相當潛力之脂溶性活性物質（如 維生素Ε )《載體，並呈現出良好的緩釋作用，而這些功 效可增加纖維於食品產業的應用，開發出具有多功能的保 徤食品。 以上所述，僅是本發明的較佳實施例，並非對本發明 作任何形式上的限制，任何所屬技術領域中具有通常知識 者，右在不脫離本發明所提技術特徵的範圍内，利用本發 明所揭示技術内容所作出局部更動或修飾的等效實施例， 並且未脫離本發明的技術特徵内容，均仍屬於本發明技術 特徵的範圍内。 【圖式簡單說明】 第一圖係為經均質處理之纖維-維生素Ε複合物之維生 素Ε體外釋放率。 第二圖係為食用植物纖維_維生素複合物之維生素Ε攜 帶力（mg/g)。 第二圖係為傅式轉換紅外線光譜（A)經均質之非水溶 性纖維；（B)未經均質之非水溶性纖維。 【主要元件符號說明】 無 15Time (8) Control group b weiwei·vitamin complex 0 26.4 ± 2·5 ν 24·7±2.4 ν 1 100±6.7w 51.7±3.2w 2 37.8 ± 3.6x 76.9 ± 5·2χ 3 28.0±1.9v 88.7 ± 6.4 y 4 26·7 ± 2.0v 55.4 ± 5_5w 5 26.4 ± 1 · 6ν 37.7 ± 3.6z -if The value of the different letters (mean ± standard deviation, η -6) in the block, with significant differences (/ >< 〇, 〇 5). The total amount of vitamin bismuth set in the test group of 2 1 4^?\ group and fiber-vitamin complex. Example 4: Estimation of the carrying capacity of vitamin ,, according to the method "in vitro vitamin Ε release test, the method described The micro-modified, vitamin-band capacity (mg/g) between different complexes was used to determine the amount of vitamin ε in the artificial gastric juice filtrate that was continuously shaken for 37 hours by 37 C ultrasonic wave as an estimate of the carrying capacity of vitamin E. The vitamin E-carrier complex of the homo-complex can carry the vitamin (9551⁄4) of the vitamin complex carried by the mixture. This result shows that the vitamin-portable fiber mixture of the complex and the homogenized fiber and the living zone Capability analysis, the results showed that homogenized fiber _ ® a large amount of vitamin E, without homogenized fiber E (M2 mg / g, while homogenized fiber - 丨 E carrying capacity was greatly improved to 1 〇 7 out of homogenization treatment Increasing Yangtao Weaving: The amount is about 9.6 times, which may be due to the average; 13 1344377 After the treatment, the fiber surface exposes more functional groups such as several groups, carboxyl groups and hydroxyl groups (second figure) ) etc. 'This enhances the carrying capacity of vitamins; this excellent vitamin E carrying capacity suggests that the water-insoluble fiber of the f-treatment can be a new type of fiber. The form of the vitamin complex has a considerable potential for fat solubility. The carrier of the active ingredient. Example 5: Four-dimensional conversion infrared spectroscopy (FTIR) analysis of the water-insoluble fiber sample (about g) mixed with (10) mg of potassium chloride, and then a part of the sample was pressed at a pressure of 167 MPa, after tableting After drying at 4 ° C for 24 hours, it was stored in a dry box for analysis. The ftir analysis was analyzed by Bruker Equinox 55 spectr_ter (BiUerica, usa) in the wavelength range of about 500-4000 · 1. The second picture is The effect of homogenization on the functional groups of water-insoluble fibers was analyzed by FTIR to homogenize the surface functional groups. The results showed that the FTIR spectra of the non-homogeneous non-water-soluble fibers were compared (third B). After homogenization, the water-insoluble fiber exposed a new C=0 absorption peak at 1655, 丨 ❶ and Η” cm 1 , and exposed a new 0-H absorption peak at 35〇〇~39〇〇(10). (third A picture ), the results show that the homogenization treatment can change the surface structure of the fiber and expose more functional groups such as carbonyl, carboxyl and hydroxyl groups, so the homogenization treatment can be applied to modify the functional groups of the water-insoluble fibers of carambola; In the nature test, the carambola water-insoluble fiber after homogenization treatment showed higher oil retention (about 2〇4 times), so it is known that the change of these functional groups further affects the characteristics of the sample and can improve it. Physicochemical properties, increased application in food. 14 1344377 π 〇 The above examples and results show that the homogenized fiber-Vitamin complex sigma can be developed as a fat-soluble active substance (such as vitamin Ε) with considerable potential. "Carriers, and exhibit a good sustained release effect, and these effects can increase the application of fiber in the food industry, and develop a multi-functional food. The above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any one of ordinary skill in the art can use the present invention without departing from the technical features of the present invention. Equivalent embodiments of the present invention may be made without departing from the technical features of the present invention. [Simple description of the diagram] The first figure is the in vitro release rate of the vitamin-vitamin complex of the homogenized fiber. The second picture shows the vitamin Ε carrying capacity (mg/g) of the edible plant fiber _ vitamin complex. The second figure is a F-transformed infrared spectrum (A) homogenized non-water-soluble fiber; (B) a non-homogeneous non-water-soluble fiber. [Main component symbol description] None 15