TWI336781B - Fluidic device and controlling method thereof - Google Patents

Description

1336781 99-3-22 IX. Description of the invention: [Technical field to which the invention pertains] This description relates to a fluid device and a control method therefor. [Prior Art] The presence of a compound or the analysis of a biochemical reaction can be measured by using a test apparatus of a plurality of types. For example, Lateral Flow Assays can be performed using a Lateral flow membrane with one or more test lines along the length. The fluid with the dissolved reagent travels from one end of the membrane to the test line by electroosmosis. The reader detects if a reaction has occurred at the test line indicating the presence or absence of certain particles in the reagent. As another example, a device having a set of microcapillaries can be used to control fluid flow in an immunoassay process. The reagent is positioned at a plurality of locations along the length of the microcapillary such that when the fluid flows in the microcapillary due to capillary forces, the fluid contacts the reagent. The reader monitors the location of the reagent to determine if a reaction has occurred. As a further example, the microfluidic wafer can be used to perform the assay by controlling the flow of fluid through the plurality of channels and chambers. The microfluidic wafer can be used with an external power source and/or pump that provides a driving force to drive the fluid. SUMMARY OF THE INVENTION The present invention provides a fluid device for performing an assay, including, for example, a vacuum pump, a gas pump, a "Broken open valves," and a "self_cl〇se valves," A control assembly for controlling fluid flow in the fluid device. In the fluid device for performing the assay proposed by the present invention, a vacuum pump of 5 1336781 99-3-22 can be used to draw the fluid in the passage in a specific direction, and a gas pump can be used to push the fluid in the passage in a specific direction. In the fluid device for performing the assay proposed by the present invention, a disconnected open valve can be used to connect two separate zones controlled by the user, and a self-closing valve can be used to automatically seal the passage after the fluid passes.

In the fluid device for performing the verification proposed by the present invention, the direct operation of the gas, the «1 type of hitting and the self-contained S = the piece of the piece make the fluid device can be made very small and portable = 1 find, rib (four) over the channel Yin = move this method includes disconnecting the first container to produce a gas in the channel from the gas pressure of the external gas, there is: the same as the first container - the first material A container breaks the door and recognizes i) 匕 — 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟 弟In the case of the first material and the present invention, the first container is made of a brittle material. The space, the gas pressure of the gas-prepared state (a) is different from the gas pressure of the gas-vehicle force, j is different from the first-container outside the Zou-container and is located at the first: capacity::: material: this first-head " 'This first material and the second material produce a gas immediately after interacting with the second material by S: 1336781 99-3-22. Provided is a fluid device comprising a first material defining a first region and a second material defining a second region, the second region being separated from the first region, and further comprising a connector coupled to the first region and the second region Between the connector, the connector comprises a brittle material and has an open end and a closed end. The open end is disposed in the second region, and the closed end is disposed in the first region. The first region is connected to the second region by the closed end and the second region The connection is set such that when the closed end of the connector is broken, the connector defines a passage from the first region to the second region. 1 The present invention provides a fluid device including a passage for an expansion portion, The expanded portion has a diameter greater than an adjacent portion of the passage, and further includes a material disposed in the expanded portion. The material has a volume that does not block the passage of the fluid prior to adsorbing the fluid, wherein the material adsorbs a portion of the fluid immediately after the volume % swells so that the expanded material blocks the passage of additional fluid through the passage. This material contains super sorbent polymer. ▲ The present invention provides a a fluid device comprising a first set comprising a first fluid, a second reservoir containing a second fluid, a primary channel, a first branch channel, a second branch channel, a first single use pump, and a second single The pump is used twice. The first branch channel is coupled to the first reservoir to the main channel, and the second branch channel is coupled to the second reservoir to the main channel. This first single use ^ is the first When a single use of the container in the pump is disconnected, the first single use pump creates a pressure differential to move one or both of the first fluid and the second fluid. When the second single use pump is in the container When disconnected, the second single causes the 2 pump to create a pressure differential to move one of the first fluid and the second fluid or two of the 7 (1) 6781 99-3-22. The present invention provides a method of controlling a fluid, including providing To enable sampling of a predetermined amount of fluid. Each of the straws includes a pass, a head, a & and a valley state, and when the container is disconnected, a pressure difference is generated in the passage, and this is done. . ^ Made of brittle material. This container defines the space within the container, due to: the gas pressure of the external gas pressure, wherein the disconnection will cause a predetermined amount of pressure difference so that the amount of fluid is absorbed by the method of controlling the fluid' Having the connector capable of the open end and the closed end of the fluid being connected to the nth portion disposed in the second region, and the closed end being disposed at the closed end of the second 够 Γ 够 = 饰 饰 , , , , , , , , 饰By using a sorbent fluid back body; through the connector through the expanded material (four) qing extra lining and using the present invention provides a control of steam, soil, the flow m contains the fluid in the channel

The material is such that the volume of the fluid attached to the fluid immediately expands. (4) The flow of the body in the passage includes the fluid material and the use of the material to adsorb the first to eighth volume of the fluid. And pulling the knife, so that the material = 1 road and the use of the material to be extruded - breaking the extra fluid through the present invention provides a method of controlling the fluid, including passing the fluid through the pass 1336881 99-3-22, This channel includes a first self-closing cymbal and a second self-closing valve. The first self-closing valve and the second self-closing valve are spaced apart from each other, and each self-closing valve includes a fluid adsorbing material that expands immediately after the portion of the adsorbing fluid. The portion of the fluid is adsorbed by using the first self-closing valve and the fluid adsorbing material in the second self-closing valve. The volume of the expanding fluid adsorbs material to block additional fluid from retreating through the passage of the passage, maintaining a predetermined amount of fluid in the portion between the first shut-off valves of the passage.

The present invention provides a method of controlling a fluid comprising breaking a first container made of a brittle material to create a force difference in the passage such that the first fluid moves from the first reservoir to the first region of the passage segment. The space inside the first two;;=, the space has a gas pressure different from the outside of the first container, or (8) includes the first material, 枓, the first-capacity!! Make the first material 舆 second material: phase = vertical "produce read. disconnected by brittle material _ -

The pressure difference in the road makes the first squad, the Gu, the second section of the passage. The partial movement of the crucible is provided by the present invention, the single-use pump for controlling the fluid, and the second single-time operation comprising the simultaneous operation of the first portion to the first passage and the suction sample fluid 7 and the first disconnection of the sample fluid A single use of the pump t - the volume of crying ° points to the second channel. The first part of the sample fluid moves from the reservoir to the = force difference, so that the second container in the second use of the pump is the second channel, and the second part is disconnected from the reservoir to the first _ ^ The force difference makes the sample fluid happy. Simultaneous operation of the third single 9 1336781 99-3-22 The pump and the fourth single use pump are used to draw the first buffer solution to the first channel and the second buffer solution to the second channel. [Embodiment] A fluid device for performing an assay may include a control component such as a vacuum pump, a gas pump, a Broken open valve, and a "self-close valve", which is used for Controlling fluid flow in the fluidic device. A vacuum pump can be used to draw fluid in the channel in a particular direction' and a gas pump can be used to push the fluid in the channel in a particular direction. A disconnected open valve can be used to connect the user controlled Two separate zones' and a self-closing valve can be used to automatically seal the passage after the fluid passes. The vacuum pump, gas pump, disconnect open valve, and self-closing valve can be made small so that the fluidic device can be made small and In the following description, individual control components can be introduced first, and then how the control components are combined to construct a description of the sputum assembly used to control the fluid in the fluid device. How to use the fluid will be described later The device performs a bioassay. Referring to Figure 1A', the container 1 can be placed in a channel 106 (or chamber defined by material) The vacuum pump % is constructed. The container 1 is closed to the area 104, the area 1 〇 4 is vacuum or has a lower gas pressure than the gas pressure in the channel 1 〇 6. Referring to Figure 1B, the container 100 can be, for example, glass. The capillary, which is disconnected immediately after the application of an external force. When the container 1 is opened, the gas in the channel 1〇6 flows into the vacuum region 104', thereby reducing the pressure in the channel contact. In this way, it can be pulled in the direction 1G8. The fluid is directed toward the channel (10) 10 99-3-22. - Figures 25A to 25C show an example of a vacuum pump using a glass capillary placed in a rubber tube. Figure 25A shows a cross section of the gas pump 41〇, The gas pump 410 has a vacuum glass capillary 416_' disposed in the rubber tube 418, wherein the tube 418 has a closed end 424 and an open end 426. Figure 25 shows a cross section of the gas pump 412, which is similar to the gas pump 41, In addition to having a rubber tube 42 with two open ends, Figure 25c shows a gas pump 412 coupled to two rubber tubes 428, wherein the rubber tube 420 has an inner diameter larger than the rubber tube 428 (to accommodate the glass wool) Tube 416) Figures 26A and 26B show an example of a vacuum pump using a glass capillary placed in a planar fluid passage. Figure 1 shows the transverse base of the vacuum pump 43 and the vacuum pump 430 has been placed on the flat substrate 434. The fluid glass 438 has a closed glass 416. The fluid channel 438 has a closed 440 and an open end 442. The planar substrate 434 is slid into the substrate 434 by a rigid material = layer 436 adjacent to the capillary, so that The user applies an external force through the layer to open the capillary. Straight * ί is not the transverse surface of the vacuum pump 432, the vacuum pump 432 is similar to the two channels 438 connected to the closed end of the closed tube with a smaller cross section to melt the glass No. - ====: Adding g heat to soften the glass from the first-closed end, the softened glass can be pinched or twisted to form the closed end of the second. 11 1336781 99-3-22 Referring to Figure 2A, the gas pump % can be constructed by placing the container 110 in a channel 106 (or chamber) defined by the material ι2. The container 11 encloses a region 1 Π which has a higher gas pressure than the gas 麈 force in the channel 1 〇 6 outside the container 11 。. Referring to Fig. 2B, the container no can be, for example, a glass capillary which is broken immediately after an external force is applied. When the container 110 is disconnected, the gas originally inside the crying chamber flows out of the container 110, thereby increasing the pressure in the channel 1〇6. In this way a force is generated that can push the fluid in direction 114 away from the passage. In this description, the term "vacuum pump" will be used to generally refer to a device that produces traction that can be used to pull fluid toward the device; and the term gas pump will be used to refer to a device that generates a pushing force that can be used to push a fluid Keep it away from the device. There are alternative ways to construct a gas pump. For example, referring to Fig. 3A', a gas pump 94 can be fabricated by placing a glass capillary ι2 partially filled with a first material 126 in a channel 124 (or chamber) containing a second material 128. The first material 126 and the second material 128 are selected such that when the material 126 and the material 128 are mixed with one another, the two will interact and produce one or more gases. For example, the first material 126 can be sodium carbonate (Na2CO3) and/or sodium bicarbonate (NaHC03), and the second material 128 can be acetic acid (ch2cooh). Referring to Figure 3B, when an external force is applied to break the glass capillary 120, the first material 126 interacts with the second material 128 and produces a gas. In this example, the gas is carbon dioxide (C〇2). The chemical reaction that occurs is: 12 1336781 99-3-22

Na2C〇3 + 2 CH2C00H 2 NaCOOCH2 + H2〇 + C〇2

NaHC〇3 + CH2COOH -> NaCOOCH2 + H2〇 + C02 Carbon dioxide increases the pressure in channel 124, creating a force that can be used to push the fluid away from the broken capillary 120. The first material 126 can be directly filled into the capillary 12〇. Referring to Fig. 27A', the first material 126 can also be attached to the wire 45A, and then the wire 450 is placed inside the capillary 120 together with the coating material 126. Fig. 27B shows an example in which the glass capillary 120 is placed in the passage 124 in the rubber tube 418. Channel 124 contains a second material 128 that can interact with first material 126 when glass capillary 120 is broken. Figure 27C shows an example of placing a glass capillary 120 in a fluid channel 438 in a planar device substrate 434. The elastic layer 436 is embedded in the substrate 434 at a position adjacent to the capillary 120, allowing the user to apply an external force via the elastic layer 436 to break the capillary 120. Referring to FIG. 4A, a gas pump 96 can be fabricated by placing the compound 130 in a glass capillary 132, sealing the capillary 132, heating the capillary 132, cooling the capillary 132, and placing the capillary 132 in the channel (or chamber). in. Compound 130 is selected as the material that produces the gas upon heating. When the capillary 132 is heated and cooled, the gas generated by the compound increases the gas pressure inside the capillary 132 (compared to the gas pressure outside the capillary 132). Examples of the compound 130 include sodium hydrogencarbonate (NaHc 3 ) and calcium carbonate (CaC 3 ). These compounds produce carbon dioxide when heated:

NaHC03 -> NaOH + C02 13 1336781 99-3-22

CaC03 CaO + c〇2 吏 is used as a sublimation material from the change of the silk state (the table shows the heating of the body of the basin sc such as NaN3 heating to produce nitrogen (2 NaN3 --> 2Na + 3N2).

Referring to FIG. 5A, a split-open valve can be manufactured by placing a glass capillary 142 between the first passage 148 and the second passage 15A. Glass wool = tube 1 = has a positioning in the first passage The open end 144 in 148, and the closed end 146 in the second channel 15''. When the glass capillary is intact, the fluid cannot move between the first passage 148 and the second passage 15〇. This is called a disconnected open wide "off" state. Stage Referring to Figure 5B', when an external force is applied to break the glass capillary 142, the connecting passage 148 is formed with the passage 152 of the passage 150. This is called the disconnected, open "open" state. The open-open 14 〇 can be used to allow initial separation of the two fluids (or fluids and a solid) and then interact at a time controlled by the user.

Fig. 28 and Fig. 28B show the use of a breakaway open to construct a low example of a low cost device for performing a test in which a fluid is irradiated with ultraviolet (UV) light. The glass capillary 142 connects the two plastic passages 46 Μ and 462. Initially, reactant 464 is included in first plastic passage 462. ^, after the tube 142 is disconnected, the reactant 464 immediately flows through the glass capillary = 2 to the second plastic channel paste. When the reactant paste capillary 142 is shown in Fig. 28β, the UV light source 466 radiates the reactant bias. Debt = Pirate 468 detects uv light passing through Reagent 464. The detector's spectrum of UV light can be used to determine the compound in the reactant bias. Fig. 28C shows the inner and outer periphery of the glass tube 14 99-3-22 having a square inner periphery and an outer periphery, allowing the ϋν light to pass through the glass capillary perpendicularly to the glass. The capillary has a fluid in the circular capillary, where a circular cross section may reflect or redirect light away from the fluid.

See ® 6Α and Figure 6Β, the super-adsorbent polymer ^Superabsorbent _ _, hereinafter referred to as "sAp" 162 can be placed; in the channel 164 to manufacture the self-closing valve (10). Initially, M2 has a smaller volume and allows fluid to flow between the first region 166 and the second region 168 in the channel 164 (Fig. 6A). This is called the "open" state of the self-closing 阙. As the fluid flows through the SAP 162, the SAP adsorbs a portion of the fluid and expands in volume, thereby blocking the passage 164 (Fig. 6B), thereby preventing further flow of fluid between the first region 160 and the second region 168. This is referred to as the "off" state of the self-closing valve.

Supersorbent polymers adsorb and retain the bulk of water or other aqueous solutions. In some instances, 'SAP can be made from chemically modified powders and cellulose and other polymers' such as poly(vinyl alcohol) PVA, poly(ethylene oxide) which is highly hydrophilic and has a high affinity for water. PEO. In some instances, the supersorbent polymer can be made from partially neutralized and lightly crosslinked poly(acrylic acid), partially neutralized and lightly crosslinked poly(acrylic acid) having a good performance versus cost ratio. The polymer can be made to have a low solids content which is then dried and ground to a white granular solid. In water, the white solid swells to a rubber gel which in some cases may include up to 99% by weight of water. 15 1336781 99-3-22 <<Look, 7A' The self-closing valve 170 can include a passage 164 having a flared portion 172 to accommodate the super-absorbent polymer 162 such that the super-absorbent (tetra) polymer 162 is not expanded prior to expansion. The flow of fluid. In order to manufacture the self-closing _ 17Q ', the adhesive layer can be applied to the inner wall of the expanded material 172, and the SAP 162 in the form of powder is subsequently pushed into the passage 164, so that the SAP 162 powder adheres to the inner wall at the enlarged portion m.

Referring to Figure 7B, as the fluid flows through the supersorbent polymer M2, the superabsorbent age #162 adsorbs a portion of the fluid and expands in volume, thereby refracting the passage 164, thereby preventing further flow of fluid through the expanded polymer. Referring to Figures 8A and 8B, supersorbent polymer 162 can be attached to lead 180 and subsequently placed in channel 164. Channel 164 can have a recessed region 182 that is coated with an adhesive to secure wire 18〇 at a predefined location. Referring to Figure 8C, as fluid flows through the supersorbent polymer 162, the polymer 162 adsorbs a portion of the fluid and expands in volume, thereby blocking the passage 164, thereby preventing further flow of fluid through the expanded polymer 162.

A self-closing valve can be fabricated by coating a wire with SAP and then placing the coated wire in a channel or tube. Self-closing valves for planar fluidic devices can be fabricated by coating a planar substrate with SAP and subsequently placing the coated substrate in a planar channel in a planar fluidic device. Referring to Figures 9A through 9C', the switch opening 190 can be made by using a glass capillary 142 and an SAP 162 positioned outside of the capillary 142 and adjacent to the capillary 142. Capillary 142 and SAP 162 are both positioned in channel 164 having a first region 166 and a second region 168. Use of broken glass 16 1336781 99-3-22 Thin tube 142 and SAP are similar to the combination of a disconnected open valve and a self-closing valve. Switching the open valve 190 allows the user to control the flow of fluid through a particular location in the channel by allowing, subsequently blocking, and then allowing fluid to pass through a particular location. Referring to Figure 9A, initially, SAP 162 has a smaller volume and does not block the passage, allowing fluid to flow between first region 166 and second region 168. Referring to Figure 9B, as the fluid passes, a portion of the fluid is adsorbed by SAp 162, thereby increasing the volume of SAP 162, thereby blocking further flow of fluid between first region 166 and first region 168. Referring to Figure 9C', when an external force is applied to break the glass capillary 142, a passageway 152 is created to allow fluid to flow between the first region 166 and the second region 168. Referring to Figures 10A through 10C, the switch width 200 can be made by using the break capillary M2 and the SAP 162 positioned inside the capillary 142. Capillary 142 has an open end 144 and a closed end 146. The open end 144 is positioned in the first passage 148 and the closed end 146 is positioned in the second passage 150. The glass capillary 142 and the SAP 162 perform a function similar to the combination of the &amp; = open valve and the self-closing valve. Turning off the on/off valve 2〇〇 enables the user to control the flow of fluid through a particular location in the channel by blocking, subsequently allowing, and then blocking the fluid from passing through the set position. 1

Referring to Fig. 10A, when the glass capillary 142 is intact, the first passage 148 is not connected to the second passage 150. I Referring to Fig. 10B, when an external force is applied to break the glass capillary ] 日 4 17 1336781 99-3-22, a passage 152 ′ is formed to allow fluid to flow between the passages 148 and 15 。. The SAP 162 initially has a small volume and does not block the flow of fluid in the passage 152. Referring to Figure 10C, as fluid flows through passage 152, portions of the fluid are adsorbed by SAP 162, causing the SAP volume to increase and block passage 152, thereby preventing fluid from flowing further through passage 152. Referring to Figures 11A through 11D, the switch-off valve can be manufactured by using a glass capillary 142, an SAP 212 positioned inside the capillary 142, and an SAP 214 positioned outside the capillary 142 (〇n_〇ff_〇n_〇ff# Valve). Glass capillaries 142, SAp212, and SAp2i4 are placed in the port 164. The glass capillary 142, SAP 212, and SAP 214 perform a function similar to the combination of an open-open valve and two self-closing valves. The switch-off valve 210 allows the user to enable, subsequently block, and subsequently =. After Ik and Ik, the fluid is passed through a specific location to control the flow of fluid through a particular location in the channel. Referring to FIG. 11A, initially, SAP 214 has a smaller volume and allows flow; flow between first region 166 and second region 168 of channel 164. • Tea Looking at Figure 11B, as the fluid passes, the portion of the fluid is increased by SAp 214 = « to increase the volume of SAP 214, thereby blocking further flow of fluid between first region M6 and second region 168. Referring to the diagram lie, when an external force is applied to break the capillary capillary 142, the path 152 allows the fluid to move between the first region (6) and the second region 168. Looking at the image UD, when the fluid flows through the SAP, the portion of the fluid is adsorbed by the 1336781 99-3-22 SAP 2^12, thereby increasing the volume of the SAp 212 and blocking the passage 152, thereby preventing the fluid from flowing further. Path ι52. Referring to Figure 12, a metering straw (Metei.ing P) 220 for drawing a predetermined amount of fluid can be constructed by using a vacuum pump 222 coupled to the straw body 224. Vacuum pump 222 includes a vacuum glass capillary 100 disposed in a straw sphere 226. To use the metering straw 22, the glass capillary 1 is broken to create an attractive force for drawing fluid into the straw body 224. When the batch of metering straws 220 are manufactured, the spheres 220 and the glass capillary tubes 100 can be made the same size. The ball 226 and the glass capillary 1 are further counted by the user to press the ball 226 to break the glass capillary 1 〇〇 4, causing the amount of deformation of the application sphere 226 required to break the glass capillary 100 for all metering straws 220. In general, it is the same. In this manner, the user can use the metering straw 220 to quickly draw a predetermined amount of fluid without having to monitor the fluid content of the straw body 224. For example, referring to Figures 21A and 21B, a metering pipette 220 can be used to quickly sample a predetermined amount of blood 370 from a patient. Referring to Figure 13, another example of a metering straw 230 includes a vacuum pump 222 and a gas pump 232. The vacuum pump 222 is similar to the vacuum pump shown in FIG. Gas pump 232 includes a glass capillary 120 filled with NafO3 and placed in a straw sphere 234 containing CHfOOH. When the glass capillary 120 is broken, 'Na2C〇3 interacts with CHfOOH to generate C〇2, thereby increasing the gas pressure in the sphere 234. The vacuum pump 222 allows the user to quickly draw a predetermined amount of fluid into the straw 230. The gas record 232 allows the user to dispense fluid outside of the straw 230. 19 1336781 99-3-22 The advantage of using gas pump 232 is that when the reaction between (1) 2 gas is generated,

The outflow of fluid when dispensing a fluid.

: When Na2C03 and CH2COOH are in the body, the user can be subjected to a controlled period of time without further careful monitoring. Another example of the MA' metering straw 24 includes a sphere (10), a middle portion 244, and a straw body 246. The intermediate portion 244 is positioned in the intermediate portion 244 by a deformable two switch open valve 248. The switch open valve 248 includes a glass capillary 142 and an SAP 162 positioned outside of the capillary 142, similar to the device shown in Figures 9A-9c. &gt; Looking at Figure 14A, in order to use the suction tube 240, the user squeezes and releases the ball 242 to draw fluid into the body 246 and the intermediate portion 244. Referring to Figure 14B, when the fluid reaches intermediate portion 244 and begins to contact SAp 248, the portion of the fluid is adsorbed by SAP 248, causing SAP 248 to expand in volume and block the fluid passage on the other side of SAp 248. In this manner, a predetermined amount of fluid 252 is drawn into the straw 240. Referring to Figure 14C, to drain fluid from the suction tube 24, the user presses the intermediate portion 244 (made of a deformable material) to break the glass capillary 142' to form a passage through the broken capillary 142. The user then squeezes the ball 242 to force fluid out of the straw 240. When a plurality of suction tubes 240 are manufactured, the tubular body 246 and the intermediate portion 244 are the same size, and the position of the switch opening valve 248 in the intermediate portion 244 is the same, so that the user can use the suction tube 240 to eliminate the need for precise monitoring of the straw. Quickly draw substantially the same amount of 20 99-3-22 in the case of a liquid content of 240. Referring to Figure 15A, a metering device 260 for receiving a fluid comprising a glass leather raft + water pre-filling; 5 262 has two branches 266a from and 266b, two self-closing rituals frP卩pq 68a and 268b' and two disconnected eight open valves 270a and 270b. White μ /=., the mother in the self-closing valves 268a and 268b - immediately after the adsorption of the fluid, 26Ra r; . ^ou ^, 1 swelled SAP. Initially, the self-closing valves 2(10)a and 268b are in an open state, and the squad is open and the valve 270a is opened.

鸠 is off. The self-closing interval and the cymbal can be similar to the self-closing interval shown in Figs. 6A to 8C. The break open valves 27A and 2 can be similar to the split open between Figs. 5A and 5B. In operation, due to capillary forces, fluid 274 is drawn into capillary tube 262 and flows through self-closing valves 268a and 26A. Referring to FIG. 1SB, when fluid 274 flows through self-closing valves 268a and 268b, portions of fluid 274 are adsorbed by SAP in shut-off valves 268a and 268b, thereby causing self-closing valves 268a and 268b to be turned off. The further flow of fluid 274 is blocked. In this manner, fluid 274 occupies a section 264 between the self-closing valves 268a and 268b of the capillary.

By moving the disconnected open valves 270a and 270b from the closed state to the open state and applying an attractive or urging force to move the fluid 274, the fluid 274 can be moved from the section 264 to other locations via the branches 266a or 266b. An advantage of the metering device 260 is that the metering device 260 can quickly sample a predetermined volume of fluid without careful monitoring by the user. Because the capillary has a small diameter, the metering device 260 can be used to accurately sample a small amount of fluid. Referring to Figure 16A, a metering device 280 that can acquire three different amounts of fluid from the sample cell 282 21 1336781 includes two initials a...per-capillary at-ends 2842' 284b and 284C. L f 二山目 has a self-closing room (for example, 286a, or 286c) and the other end has a straight coffee p^r. Each straight button f has a direct clerk (see 288a, 288b or 288c) 〇(1):: There are ~ face capillaries. Initially, the self-closing valve is in an open state.

Referring to Fig. 16B', the ancient user disconnects the vacuum glass of the vacuum pump 288a from the capillary line 4' to extract a predetermined amount of liquid into the capillary 284a. When passing through the self-closing valve 286a, the sAp expansion in the self-closing valve 286a causes the self-closing valve 286a to enter a closed state, thereby preventing the fluid from moving further through the self-closing valve 286a. Similarly, a predetermined amount of fluid can be drawn into the capillaries 284b and 284c by opening the vacuum pump 288b with the vacuum capillary of the 288e towel. The amount of fluid that is drawn into the capillaries 284a through 284c is determined by the volume of the capillaries in the vacuum pumps 288a through 288c. The amount of fluid that is drawn into the capillaries 284a through 284c can be the same or different.

Referring to Fig. 17A', a device 29 for a two-step assay can be fabricated by using a combination of a vacuum pump, a disconnected open valve, and a self-closing valve. The two-step assay requires cleaning with a buffer after rapid binding of the reagent. One end of the channel 3〇2 is coupled to the sample cell containing the sample 3〇〇 via the self-closing valve 296, and the other end of the channel 302 is coupled to the first vacuum pump 292a. Channel 3〇2 is coupled to channel 308' and channel 308 is coupled via a disconnected open valve 294 to a reservoir containing buffer 298. Channel 3〇2 is also coupled to channel 304, which is coupled to second vacuum pump 292t) and third vacuum pump 292c. Channel 304 includes a binding and/or sensing region 〇6, and the binding and/or sensing region 306 package 22 1336781 99-3-22 includes reagents for binding or sensing compounds in sample 300. Device 290 is operated in a manner such that sample 300 is aspirated toward bonding and sensing region 306 to cause a reaction to occur, followed by buffer 298 to the bonding and sensing region 306 to clean the bonding and sensing regions. Referring to Figure 17B, vacuum pump 292a is activated to generate an attractive force that draws sample 300 toward vacuum pump 292a and draws sample 3〇〇 to a portion of passage 302 between vacuum pump 292a and self-closing valve 296. When the sample 3 〇〇 flows through the self-closing valve 296, a portion of the sample is sucked by the sap in the self-closing valve 296, thereby bringing the self-closing valve 296 into a closed state. Referring to Figure 17C, the break open valve 294 is actuated to change the valve 294 to the open state. The vacuum pump 292b is activated to generate an attractive force for drawing the sample 300 and the buffer 298 toward the vacuum pump 292b. Vacuum pumps 292a and 292b are designed such that the sample 3 will stop at the bond and sensing zone 306 after the pump is activated. After a period of time, vacuum pump 292c is activated to move sample 300 out of zone 306 and buffer 298 is passed through zone 306 and wash zone 306. The above example provides an incubation time to allow the compound in sample 3〇〇 to react with the reagent in zone 306 prior to buffer 290 wash binding and sensing zone 3〇6. If the reaction at zone 306 is fast and the incubation time is unnecessary, then vacuum pump 292b can be made larger and vacuum pump 292c can be omitted. When the vacuum pump 292b is activated, the sample rapidly flows through the bond and sensing region 306' and then the buffer 298 cleans the bond and sense region 306. Referring to Figure 18A, a device 310 for a two-step assay can be made by using a vacuum pump, a disconnect open valve, a 23 1336781 99-3-22 shut-off valve, and a combination of gas pumps. The two-step assay requires slow binding of the reagents. Afterwards, it is washed with a buffer. Device 310, similar to device 290, has a channel 302 that is connected to two channels 304 and 308. Channel 302 is coupled to sample 300 via self-closing valve 296. Channel 308 is coupled to buffer 298 via a disconnect open valve 294. Channel 304 includes a bond and sense region 306. One end of the passage 304 is coupled to the break open valve 312. The gas pump 314 is coupled to the buffer 298. The difference between the device 310 and the device 290 is that the device 310 does not use the vacuum pump 292b to draw the sample 300 and the buffer 298 toward the bonding and sensing region 306, but instead uses the gas pump 314. The sample 300 and buffer 298 are pushed toward the zone 306. Referring to Figure 18B, in order to perform a two-step assay, vacuum pump 292a is activated to draw sample 300 into the channel. Valve 296 enters a closed state after the sample has flowed through self-closing valve 296. Referring to Fig. 18C, the open type open valves 294 and 312 are actuated to change the valve to the open state. Gas pump 3.14 is activated to generate gas over a period of time to push sample 300 and buffer 298 through bonding and sensing region 306. Since the gas pump 314 generates gas over a period of time (the reaction between the gas generating compounds takes a certain amount of time to complete), the sample 300 can slowly pass through the bonding and sensing regions 306, so that the binding reaction occurs slowly. Referring to Fig. 19A, a device 320 for a three-step test can be fabricated by adding a reservoir containing a second buffer 324 and a channel 322 to the structure shown in Fig. 17A. The buffer is cleaned. In order to perform the speed-binding reagent and use two to make the sample 300 flow to the channel 3〇H check 'start true line 292a 296, wide 296 changes to off=when the sample flows through the self-closing valve skin f Γ straight ί The valve 294 is opened to change it to the suction sampling sample 3D and the first-buffering month direction 'binding and sensing area _

The valve 326 is opened in a view to change it to a second, a second, a second, a second, and a second buffer (four). In this manner, the reaction at zone 306 was washed with two different buffers. The device for use in the assay requiring three or more steps can be constructed by splicing additional buffer or sample and adding a corresponding number of vacuum pumps to the end of the 304.

Referring to Figure 20, the module 33 can be constructed to perform multiple analyte assays. The module includes a sample cell for sample _ and three chambers 332a, 332b, and 332c, each containing an analyte for binding and sensing a sample of the compound. The components used to perform the assay associated with the first analyte in chamber 332a are described below. Chamber 332a is coupled to sample reservoir 282 via passage 342a and self-closing valve 344&amp; The passage 342a is coupled to the first buffer 350a via a self-closing valve 346a and a split open width 348a. Channel 342a is coupled to second buffer 356a via self-closing 352a and disconnected open valve 354a. The passage 342a is coupled to the 25 1336781 99-3-22 second buffer 362a via a self-closing valve 358a and a disconnected open valve 360a. Chamber 332a is also coupled to vacuum pumps 334a, 336a, 338a, and 340a. To perform the palpitations, the attendant vacuum pump 334a draws the sample 300 toward the chamber 332a to allow the compound in the sample 300 to react with the analyte 332a. After a certain amount of sample flows through the self-closing valve 344a, the valve 344a changes to the closed state. The first buffer 35〇a is flushed through the chamber 332a by actuating the open type open valve 34 such as (changing the valve to open) and the second vacuum pump 336a. After the -quantity-buffer 35〇a flows through the self-closing valve 346a, the valve 346a is changed to the closed state. The second buffer 356a is flushed through the chamber 332a by opening and closing the opening valve 354a (changing the width to the open state) and the third vacuum pump 338a. After a certain amount of the second buffer 356a flows through the self-closing valve 352a, the valve 352a is changed to the closed state. In a similar manner, the third buffer is flushed through the chamber 332a by actuating the break open valve 36〇a (changing the valve to the open state) and the fourth vacuum pump ground. After the third buffer 3 to be metered must flow through the self-closing valve 358a, the valve 358a is changed to the closed state. Execution of the second and third analytes associated with the chambers 332b and 332e may be performed simultaneously with the chambers, the chambers, and the chambers 332c, similar to performing the assay associated with the first analyte in the chamber 332a. The first, second and third analyte related assays. The following are the applications of vacuum pumps and gas pumps for performing biological assays. 22A and 22B show a garment 380 garment 380 for performing a fast response colorimetric assay comprising a channel with one end of the channel 384 coupled to 26 1336781

The sample cell 382 and the other end coupled to the vacuum pump 9 core sample cell 382 can hold a fluid such as blood or urine. Channel 384 includes test zone 386, which has a test line that changes color immediately after a compound is detected. True Two pumps 90 start 岈 can quickly sample the fluid in the pool 3 8 2 through the test zone 386. By reading the color of the test line, the user can quickly determine if a fluid is present or does not have a compound. Figures 23A and 23B show a package 390 for sampling filtered fluid. The device 39A includes a channel 384 coupled to the sample cell 382 at one end and coupled to the vacuum pump 90 at the other end. The filter membrane 392 is placed in the sample 382. The vacuum pump 90 can be quickly aspirated to sample the reservoir 382 to allow fluid (e.g., blood 382_a) to pass through the filter membrane 392 to produce filtered fluid (plasma 382_b) that is drawn into the channel 384. FIG. 24A through FIG. 24C show a squad for a slow colorimetric assay. The sputum 400 includes a sample cell 402 coupled between the gas smudge 404 and the channel 384. Channel 384 has a test zone 386, which has a test line that changes color immediately after detecting a certain compound. j = Work using the test 400, placing the sample fluid 406 in the sample cell 4〇2:, male, sealing tape 408 seals the opening of the sample cell. Gas pump 404 is activated to generate gas that moves sample fluid 406 through test zone 386. Since the gas pump 4 产生 generates gas during a period of time, the sample fluid 4 〇 6 travels through the test zone during the section , 4, thereby allowing the slow ratio ^ 4 386-a to be performed. "For a specific embodiment of the present invention, please refer to Figs. 29 and 29b, by using self-closing read (SLV) and open-type open valve (BOV). An apparatus 500 for performing an antibody assay on a blood sample. The apparatus 500 includes a blood sample pool 501, a washing buffer well 503, a metering region and a marker, and an antibody (Metering). Zone and labeled antibody region 505, Diagnostic Zone (antibody array) 507, disconnected open (BOV) 509, waste well 511 and self-closing valve (SLV) 513. The metering region and the marker antibody region 505 have a channel connecting the blood sample pool 501 and the flush buffer pool 503, while the self-closing wide (SLV) 513 is located between the channels, while the diagnostic region 5〇7 has another The channel is connected at one end to the center of the metering area and the marker, antibody area 5〇5, and the other end is connected to the waste well 511 through a disconnected open valve (B〇V) 5〇9. 500 pairs of blood samples for antibodies (Andb〇d force The method of the assay is shown in Figures 30A, 30B and 3〇c. First, the blood sample 502 is loaded into the blood sample cell 501, and then the self-closing valve (SLV) 513 proposed in the foregoing embodiment of the present invention is used. Using Capmary force, the blood is drawn into the channel of the metering region and the marker antibody region 505, as shown in Figure 30A, 502a. Then, the flushing buffer is loaded into the flushing buffer pool 5〇3, and this is The rinsing buffer also flows into the metering area and the channel labeled with the antibody area 505, as shown in Fig. 3〇3, for example, and the blood is then squeezed into the diagnostic area 507, as shown in Fig. 5〇%. Then, referring to FIG. 30C', after the disconnected open valve (BOV) 509 is disconnected, the blood is further drawn in through the diagnostic area 5〇7, as shown in the figure 502c' at this time in the diagnostic area 5〇7 The inner antibody array (Eight Addition 28 1336781 99-3-22 ai ray) will bind to the antigen in the blood (Aniigent) and the rinse buffer will flush the inactive blood to the waste pool 511. Yet another embodiment of the invention, see FIG. 31, which can be used by A combination of a shut-off valve (SLV) and a disconnected open valve (BOV) to make a device for performing an antibody assay on a liquid sample. The device includes a blood sample pool 601, a rinse buffer pool 603, and a diagnostic region. (Connotation Antibody Array) 607, Disconnected Open Valve (Β〇ν) 609, Waste Pool 611 # and Self-closing Valve 613. The gold sample cell contains a membrane with blood cell removal, which can be used to filter and drop blood cells when inhaled. This diagnostic zone has a passageway connecting the blood sample cell 601 and the flush buffer pool 603, while the other end is connected to the waste pond 611 via a break open valve (B〇V) 6〇9. The self-closing valve (SLV) 613 is the amount of blood that can be controlled to flow into the diagnostic zone 6〇7 before the blood sample cell 601 enters the channel, and prevents blood from flowing back to the blood sample cell 601. After the disconnected open valve (BOV) 609 is disconnected, the liquid is first filtered through the membrane of the removable blood cells, and then further inhaled into the diagnostic area 607, at this time, the antibody array in the diagnostic area 607. It will bind to the antigen in the blood (Antigent), while the flushing buffer 6〇3 will flush the inactive blood into the waste pool 611. Some examples have been discussed above, but other implementations and applications are also within the scope of the following claims. For example, in the vacuum pump 90 of Figures 1A and 1B, the container 1 may contain a low pressure region rather than a vacuum region. As long as the gas pressure inside the vessel 1 is lower than the external gas pressure of 336781 τ /J on the vessel 1 〇〇 29, when the vessel 100 is disconnected, the pressure in the vessel 1 of the vessel 1 will decrease, thereby generating Facing the container 1 in one direction: = attractiveness of the body. The capillaries described above can be replaced by capillaries that have been broken with other enamel materials, such as 'New Zealand, Quartz, and Quartz, Broken Glass, and Plastic, either or both. f Figure 1 is a schematic diagram of real skills. = and Figure 2 is a schematic diagram of gas millet. Circle 3Α and Figure 3Β are gas 鳢 Figure 4Α is a schematic/not intended for gas pump. Figure 4 is a table of materials. The picture shows a schematic diagram of the closing and closing valve of the 7r. Fig. 7B and Figs. 8A to 8C are schematic views showing the opening of the switch from the figure. Cutout. Figure 12 is a schematic view of the metering straw = valve closed. = for the metering straw

^ 6A and Fig. 16B Fig. 7-8 to Fig. 17 The intention of the CA 裒 裒 图 UA UA UA UA UA UA UA UA Figure I9AJ19C2 is a schematic diagram of the apparatus for step verification. a schematic diagram of the device for the two-step verification. 30 1336781 99-3-22 Figure 20 is for multiple

21A and 21B=: Schematic diagram of a fixed module. straw. Metering for sampling blood from a patient Figure 22A and Figure 22B are schematic views for use in a setting.仃 仃 仃 反 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图 图Figures 24 through 24C are the intent of the apparatus for performing a slow colorimetric assay.

25A to 25C are schematic views of a vacuum pump. 26A and 26B are schematic views of a vacuum pump. 27A to 27C are schematic views of a self-closing valve. 28A and 28B are schematic views of a disconnected open valve. - Figure 28C shows a cross section of a Boma hair dryer with a square inner perimeter and an outer perimeter. g Figures 29A and 29B illustrate a device for performing an antibody assay on a liquid sample by using a combination of a self-closing valve (SLV) and a break-open valve (B〇v) in accordance with an embodiment of the present invention. . Figures 30A, 30B and 30C illustrate a schematic view of the operation of the blood test device for the blood sample of Figure 29A. Figure 31 illustrates a device for making an antibody assay for a blood sample by using a combination of a self-closing valve (SLV) and a disconnected open valve (BOV) in accordance with another embodiment of the present invention. 1336781 99-3-22 [Description of main component symbols] 90: Vacuum pump 92: Gas pump 94: Gas pump 96: Gas pump 100: Container 102: Material 104: Area 106: Channel 108: Direction 110: Container 112: Area 114: Direction 120: Glass Capillary 124: Channel 126: First Material 128: Second Material 130: Compound 132: Glass Capillary 140: Disconnected Open Valve 142: Glass Capillary M4: Open End 146: Closed End 148: First Channel 32 1336781 99-3-22 150: second passage 152: second passage 160: self-closing valve 162: super adsorbent polymer (SAP) 164: passage 166: first region 168: second region 170: self-closing wide 172 : enlarged portion 180: wire 182. recessed area 190: switch open valve 210: switch on and off valve 212: super sorbent polymer (SAP) 214: super sorbent polymer (SAP) 220: metering pipette 222: metering pipette 224 : straw body 226 : sphere 228 : tube body 230 : straw 232 : gas pump 234 : straw tube 240 : metering straw 33 1336781 99-3-22 242 : sphere 244 : intermediate portion 246 : straw tube body 248 : straw tube body 260: metering device 262: glass capillary 264 Section 266a: Branch 266b: Branch 268a: Self-closing valve 268b: Self-closing valve 270a: Open-type opening valve 270b: Open-type opening valve 274: Fluid 280: Metering device 282: Sample cell 284a: Capillary 284b : Capillary 284c: Capillary 286a: Self-closing valve 286b: Self-closing valve 286c: Self-closing valve 288a: Vacuum valve 288b: Vacuum valve 34 1336781 99-3-22 288c: Vacuum valve 290: Device 292a: First vacuum pump 292b: Two vacuum pump 292c: third vacuum pump 294: open type open valve 296: self-closing valve 298: buffer 300: sample 302: channel 304: channel 306: bonding and/or sensing zone 308: channel 310: device 314: gas Pump 320: device 322: channel. 324: second buffer 326: open type open valve 330: module 332a: chamber 332b: chamber 332c: chamber 334a: vacuum pump 35 1336781 99-3-22 336a second Vacuum pump 338a Third vacuum pump 340a Fourth vacuum pump 342a Channel 344a Self-closing valve 346a Self-closing valve 348a Disconnecting opening valve 350a First buffer 352a Self-closing valve 354a Disconnecting opening valve 356a Second buffer 358a Self-closing valve 360a Disconnected Valve opening 362a of the third buffer 370: blood 380: apparatus 382: a sample cell 382-a • Blood 382-b:. Plasma 384: Channel 386: test area 386-a: colorimetric assay 390: 392 means: filtration membrane

36 1336781 99-3-22

400: Device 402: Sample cell 404: Gas pump 406: Sample fluid 408: Sealing tape 410: Gas pump 412: Gas pump 416: Vacuum glass capillary 418: Tube 420: Rubber tube 424: Closed end 426: Open end 428: Closed End 430: Vacuum pump 432: Vacuum pump 434: Planar substrate 436: Elastic layer 438: Fluid channel 440: Closed end 442: Open end 444: Fluid channel 450: Wire 460: Plastic channel 462: Plastic channel 37 1336781 99-3-22 464 Reagent 466: UV light source 468: detector 500. Apparatus for antibody testing of blood samples 501 601. Blood plate pool (bi〇〇cj sampie weu) 503 603 · Washing buffer well (washing buffer well 505: Metering zone and labeled antibody region 507, 607. φ Dicostic Zone (antibody array)

Antibody array) 509, 609 : Disconnected open valve 511, 611 : waste well 513, 613 : self-closing valve (SLV)

38

Claims (1)

1336781 99-9-8 X. Application for patents: ^^ 1. A fluid control method comprising: arranging a trough in a space formed inside a passage, the body being at least partially The elastic material is composed of the brittle material, and is disposed at a position close to a portion of the main body of the passage which is composed of the elastic material; Controlling the flow of fluid in the passageway, including breaking the first container by applying an external force to a portion of the body of the passage from the body of the passage, and creating a pressure difference in the passage Causing the fluid to move in the passage, wherein the first container (a) defines a space within the first container, the space having a gas pressure different from a gas pressure outside the first container, Or (b) comprising a first material, the first material being separated from the second material located outside the first container before the first container is disconnected, the first material and the second The daily replacement material is selected such that a gas is generated immediately after the first material interacts with the second material. 2. The fluid control method of claim 1, wherein the space in the first container has a pressure higher than the pressure of the first container. 3. The fluid control method of claim 2, wherein the controlling the flow of the fluid comprises introducing the body in the passageway away from the first container that is broken. The fluid control method according to claim 1, wherein the space in the first container has a lower than the first container outside the 39 丨 1336781&quot;1ι8 day correction replacement page The pressure of the pressure. The fluid control method of claim 4, wherein controlling the flow of the fluid comprises attracting the fluid in the passage to be disconnected toward the fluid The first container. 6. The fluid control method of claim 5, wherein the fluid comprises blood, and controlling the flow of the blood comprises passing the blood through a filter to block blood cells and allowing plasma to pass through. The filter enters the channel. 7. The fluid control method of claim 5, further comprising performing a colorimetric assay when the fluid flows in the channel. 8. The fluid control method of claim 5, further comprising a first trough, the second container (a) defining a space in the second container 'the space having a higher than the first a gas pressure of a gas pressure outside the second container, or (b) comprising a third material that is separated from the fourth material before the second container is disconnected 'the third material and the fourth The material is selected such that a gas is produced as soon as the third material interacts with the fourth material. 9. The fluid control method of claim 8, wherein controlling the flow of the fluid comprises pushing the fluid in the passage away from the second container. 10. The fluid control method of claim 5, wherein controlling the flow of the fluid comprises preventing additional fluid along a certain fluid by using a fluid adsorbing material that expands immediately after the portion of the fluid is adsorbed. The direction moves through the channel. 1336781 日修修绂11. A fluid device comprising: a passage; and a first container disposed in a space formed inside the passage, wherein the body of the passage is at least partially composed of a bomb (four) mass, And the first container is made of a brittle material and is disposed at a position close to a portion of the body of the passage which is composed of the elastic material, Φ parameter = external force in the main material of the channel a portion of the elastic material=, and when the first container is opened, a pressure difference is generated in the first volume passage, wherein the first container (4) is a space within the container, the space having a different The gas pressure of the body pressure, or (8) including the first material-container U material being separated from the first-thickness material before the first container is disconnected, the first material and the _ material and the second material phase: such as = = 1) 1 two _ two: two capacity: =:::: Immediately after the gas is produced. 4. The second material interacts with the fourth material, wherein the fluid device of the first item has a higher ratio than the outer portion of the first container. 1336781 99-9-8 months The pressure of the pressure, and the second container includes a first material, the second material being separated from the second material before the second container is broken, the first material and the second material The fluid device is selected to cause the first material to interact with the second material, and the fluid device according to claim 14, further comprising filtering the thin membrane to block blood cells and allowing The fluid device of claim 11, wherein the brittle material comprises at least one of the following materials: quartz, glass, ceramic, and quartz, glass, Tao Jing, and the like. a composite of two or more of the plastics. The pressure of the pressure is described, and the space in the second container has a pressing force lower than the dust force outside the first barn. 14. The fluid device of claim 12, wherein the space in the first container has a fluid composition as described in item 11 of the first closed container. The channel is defined by a wall made of a flexible material;;: 1 The fluid device described in the application, wherein the L device comprises a material that generates a gas when heated. The fluid device described in the above paragraph (4), wherein the first device comprises a material that sublimes from a solid state to a gaseous state upon heating. a fluid device comprising: a first sample cell containing a first fluid; a first reservoir containing a second fluid; a primary channel; 1336781 H i [first branch channel, said first branch The channel is coupled to the main channel to the main channel; the second branch channel is coupled to the collector to the main channel; The first single use pump, the first single use pump generates a pressure differential to move the fluid and one or both of the second fluid when the first single use pump is disconnected And wherein the first single use pump (a) defines a space within the first single use pump, the space having a gas pressure different from a gas pressure external to the first single use pump, or (8) including the first material, Day correction replacement IT second single use pump, the second single use pump generates a pressure differential to move the first fluid and the second fluid when the second single use pump t is disconnected One or both of the above; (4) face-to-single-single-phase separates the second material from one of the ones of the single-use pump before the county is disconnected, the first material and the first The second material is a bribe (4) - the gas is generated immediately after the material interacts with the second material. 21. The fluidic device of claim 2, further comprising a self-closing valve, the self-closing _including initially having a body _ such that the first fluid can pass the material, the material Advancing the portion of the first fluid increases the regime to prevent the fluid from entering the passage of the valve. 22. The fluid device of claim 2, wherein the valve further comprises a connector made of a sturdy material, wherein the replacement splicer is intact when the continuation date The valve prevents the first fluid from entering the channel and when the connector is disconnected, a passage is created to allow the first fluid to enter the main channel. 23. The fluid device of claim 20, further comprising: a second reservoir, the second reservoir containing a third fluid; and a third branch channel, the third branch channel The third reservoir is coupled to the main channel. The fluid device of claim 20, further comprising a sensing region coupled to the main channel, the sensing region comprising determining whether a specific material is present in the first A reagent in a fluid. 25. A fluid control method comprising: providing a plurality of straws to enable sampling of a predetermined amount of fluid, each straw comprising: a channel, and a container, the container creating a pressure differential in the channel when the container is disconnected, The container is made of a brittle material that defines a space within the container 'the space has a gas pressure less than a gas pressure outside the container' wherein disconnecting the container produces a predetermined amount in the channel The pressure differential is such that a predetermined amount of fluid is drawn into the passage. A fluid control method comprising: breaking a first container made of a brittle material to create a pressure difference in the passage such that the first fluid moves from the first reservoir to the first/section of the passage, The first container (〇 defines a space in the first container, the space has a gas different from the pressure of the gas outside the first container 99-9-8 Λ - 容器 曰 - - a container break includes The first material of the first material is separated from the second material located outside the first container and the second material is selected such that the second material is mutually Immediately after the action, a gas is generated; a second container made of a brittle material is opened to create a pressure difference in the passage such that at least a portion of the n fluid moves through the second section of the passage. The fluid control method of claim 26, further comprising disconnecting a first valve made of a brittle material to generate a first passage, the first bypass connecting the first reservoir to the passage, and the second The reservoir contains a second fluid. The fluid control method of claim 27, wherein the pressure difference generated by opening the second container causes the second fluid to move from the second reservoir to the channel 29. The fluid control method of claim 28, further comprising disconnecting a second valve made of a brittle material to create a second passage, the second passage connecting the third reservoir To the channel, the third reservoir contains a third fluid. The fluid control method of claim 29, further comprising breaking a third container made of an inert material to produce a pressure difference causes the third fluid to move from the third reservoir to the second section of the passage. 45 1336781 l9-9-8 ~ 'Ping Moon Day Correction Replacement Page 31 · If applying for a patent The fluid control method of claim 26, wherein the second section of the channel comprises a reagent for determining whether a specific determination material is present in the first fluid. 32. The fluid control method of item 26, wherein a container defining a space within the first container, the space having a gas pressure lower than a pressure of a gas outside the first container, 33. The fluid control method of claim 1 wherein The second container defines a space within the second volume, the space having a gas pressure that is lower than a gas pressure of the second chamber. The fluid control method of claim 32, wherein the second container defines a space in the second container, the space and the force 'the gas pressure (4) is higher than the second container External gas = door ί: includes the first, the first material = the 'the first material> and the second material the body. Returning to the first material, the material is directly reacted with the second material to generate a gas control method, comprising sampling 3ΓΓΓ 岐 and 第二 a second single use of the pump to absorb the channel and sucking the first force f of the sample flow Disconnecting the first-single-use ―--partial self-reservoir H moving JL the second H-th use of the H-th sample W-series to open the first person using the pump to the first (four) Producing a pressure difference such that the sample and the second portion move from the difficult 11 to the second pass, and simultaneously shoot the third material, such as the use of the four substances, to absorb the liquid to the liquid Passing the first channel and sucking the second buffer solution to the space where the 'the first-single-doing (8) defines the first-single-seven-in-station' space is different from the first-single use The gas pressure of the body pressure, or (5) comprising a first material, the first material being separated from the second material located outside and outside the pump before the first single use pump is disconnected, the first material - two t Material is selected such that the first material interacts immediately with the first material Producing a gas, the household dfi uses a pump (8) to define that the second single use pump portion has a different number than the second single use pump external body force ' or (8) includes the third material' Separating the third material from the second fourth material and the third material and the fourth material containing the same material before using the $=-single pass The New (four) method, the third buffer solution to the ί single use is to draw the second channel. μ receives and records the fourth _ solution to the fluid control method described in item 35 of the patent scope, and the reversal page of the re-editing of the first single-use pump includes the fifth single operation simultaneously with the first single-use pump The pump is used to draw the third portion of the sample fluid to the third passage, and the sixth single use pump is operated simultaneously with the third single use pump to draw the third buffer solution to the second passage. 1336781 99 03 22 om 93⁄4 ^ )f OOCSICNI CSJL 03⁄4 cvl 1336781 Year of the month correction replacement page 99 03 22 3 inch i s® 3® 2801336781 286. 288α 286c 284c 288c Ii ^ 32 80 66 1336781 Co 06CSI Year Month Day Correction Replacement Page 99 03 22 II Ο ο 〇 Η aa® νζί 1336781 ίο Pi 0063 ο ο scsl 00£ 99 03 22 GQ 00 τ — Η olro 1 CO, CM &lt;3 g3r 7 — 〇oo □DO r〇sro OVA 90ro CSIIC 0 A0CQsr^r 1336781 Year of the month revised replacement 99 03 22 inch 6CSI SS 93⁄4 t § 03⁄4 I sro Λ08 ονΛ small qacsl乂_ 026CNI_ 36L ooro AOS inch 6csli oCNro I Ί oacvl gCNl sa00'-OVA 90ro m OVA Aos t6CNI丄963 A— ooro inch CNJroJ 93⁄4 ΐ X 03⁄4 I Dacvl OVA 80roIS ii 907 0 CNl义SS qaCSI/olVA 3CVJ6CSJ V6L H 1336781 Year Month Correction f-Forming 99 〇3 Figure 21A 220 382 Figure 21B 386 384 380 90 VAC Figure 22A 386 -A-, I I VAC Figure 22B 1336781 date correction replacement page Figure 23A 99 03 go VAC 382-b Figure 23B 404 GAS 2 \ /^406 Figure 24A 408 408 N II IMMMNMIIMIII 1 | bAb &gt; y//a////\1 1 1 ^ 406 Figure 24B 404 386 ) &lt;-A-» II INIIIIIlllllllJU GAS —&gt; wii Figure 24C 386^α 1336781 Day correction replacement page 99 03 22 410 Figure 25C Figure 26A Figure 26B 1336781 Month Day Correction Replacement Page 1 99 03 22 Figure 27A Figure 27B 436 Figure 27C 1336781 Year, month and day correction replacement page 99 03 22 460 142 46 bucket Figure 28A UV 466 468 Figure 28B 1336781 22169TV/J 142 Figure 28C 1336781 G9 〇3 ^ 1336781 99-3-22 V. SUMMARY OF THE INVENTION: A fluid device for performing a test may include, for example, a vacuum pump, a gas pump, a Broken open valve, and a self-close valve. a control assembly for controlling fluid flow in the fluid device. A vacuum pump can be used to draw fluid in the channel in a particular direction&apos; and a gas pump can be used to push the fluid in the channel in a particular direction. A disconnected open valve can be used to connect two separate zones controlled by the user&apos; and a self-closing valve can be used to automatically seal the passage after the fluid has passed. The vacuum pump, gas pump, disconnect open valve, and self-closing valve can be fabricated in a small volume so that the fluid device can produce a small and portable device. Sixth, English invention summary: A fluidic device for performing assays can include control components such as vacuum pumps, gas pumps, &quot;broken open valves," and "self-close valves" for controlling the flow of fluids in the fluidic device. The vacuum pump can be used to Pull a fluid in a specific direction in a channel, and the gas pump can be used to push a fluid in a specific direction in a channel. The broken open valve can be used to connect two separate regions at the control of a user, and The self-close valve can be used to automatically seal off a channel after passage of a fluid. The vacuum pumps, gas pumps, broken open valves, and self close valves can be made small in volume so that the fluidic device can be made as a small and portable device. VII. Designated representative circle: 3 1336781 99-3-22 (1) The representative representative figure of this case is: Figure (20). (2) The symbol of the symbol of this representative figure is simple: 282: Sample pool 300 : Sample 330: Module 332a: Chamber 332b: Chamber 332c: Chamber 334a: Vacuum pump 336a: Vacuum 338a: vacuum pump 340a: vacuum pump 342a: channel 344a: self-closing valve 346a: self-closing valve 348a: open-type opening valve 350a: first buffer 352a: self-closing valve 354a: open-type opening valve 356a: second buffer 358a: self-closing valve 360a: open type open valve 362a: third buffer liquid 8. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: none