CN101109759A - Fluidic device and control method thereof - Google Patents
Fluidic device and control method thereof Download PDFInfo
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- CN101109759A CN101109759A CNA200710136828XA CN200710136828A CN101109759A CN 101109759 A CN101109759 A CN 101109759A CN A200710136828X A CNA200710136828X A CN A200710136828XA CN 200710136828 A CN200710136828 A CN 200710136828A CN 101109759 A CN101109759 A CN 101109759A
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Abstract
A fluidic device includes a first material defining a first region, a second material defining a second region that is separated from the first region, and a connector coupled between the first region and the second region. The connector includes a brittle material and has an open end and a closed end, the open end being disposed in the second region, the closed end being disposed in the first region. The first region is closed off from the second region by the closed end of the connector. The connector is configured such that when the closed end of the connector is broken, the connector defines a passage from the first region to the second region.
Description
Technical field
The present invention relates to fluid means and control method thereof.
Background technology
Can use the proving installation of many types to detect the existence or the analysis of biochemical reaction of compound.For example, can use the cross-current film (Lateral flowmembrane) that has one or more p-wires along its length to carry out cross-current calibrating (Lateral Flow Assays).Fluid with reagent of dissolving marches to p-wire by electro-osmosis (Electro osmosis) from an end of film.Read taker detecting p-wire place and whether react, have or do not exist some particle in its indicator.As another example, the fluid that can use the device with one group of microscopic capillary to control in the immunoassays processing flows.Reagent is positioned a plurality of positions along the length of microscopic capillary, makes that fluid contacts with reagent when fluid flows in microscopic capillary owing to capillary force.Whether read the site that taker monitoring reagent is positioned at reacts to judge.As another example, flow by the flow through fluid of a plurality of raceway grooves and chamber of control, can use micro-fluid chip to carry out calibrating.Can be with providing external power source and/or pump to use micro-fluid chip in order to the driving force of drive fluid.
Summary of the invention
The present invention proposes a kind of fluid means that is used to carry out calibrating, comprise the Control Component such as vacuum pump, gas pump, " breakaway-element is opened valve (Broken open valves) " and " self-closing valve (self-close valves) ", its fluid that is used for controlling fluid means flows.
Carry out the fluid means of calibrating in proposed by the invention being used to, can use vacuum pump along the fluid in the specific direction traction raceway groove, but and the using gases pump come to promote fluid in the raceway groove along specific direction.
Carry out the fluid means of calibrating in proposed by the invention being used to, can use breakaway-element to open valve and connect two isolated areas by user's control, and can use self-closing valve at fluid through sealing raceway groove automatically later.
Carry out the fluid means of calibrating in proposed by the invention being used to, vacuum pump, gas pump, breakaway-element open valve and self-closing valve can manufacture the very little element of volume, makes fluid means can be fabricated to the very little and portable device of volume.
The invention provides a kind of method of controlling fluid, in raceway groove, flow in order to the control fluid.The method comprises disconnection first container to produce pressure differential in raceway groove, make fluid mobile in described raceway groove, and first container is made by hard brittle material.This first container (a) defines the space in first container, the space has the gaseous tension of the gaseous tension that is different from first external container, or (b) comprise first material, this first material is separated with second material before first container disconnects, and first material and second material through selecting so that after first material and the interaction of second material, produce gas immediately.
The invention provides a kind of fluid means, comprise the raceway groove and first container.In first container, when first container disconnected, first container produced pressure differential in raceway groove.And first container is made by hard brittle material.And this first container (a) defines the space in first container, this space has the gaseous tension of the gaseous tension that is different from first external container, or (b) comprise first material, this first material is separated with second material before first container disconnects, this first material and second material through selecting so that produce gas immediately after first material and the interaction of second material.
The invention provides a kind of fluid means, comprise first material that defines the first area and second material that defines second area, this second area and first area are separated.And comprising that also connector is coupled between first area and the second area, this connector comprises hard brittle material and has the open end and closing end.This open end is placed in the second area, and closing end is placed in the first area.The first area cuts off by the closing end and the second area of connector.Connector through setting so that when the closing end disconnection of described connector, connector defines from the first area to the path of second area.
The invention provides a kind of fluid means, comprise the raceway groove of dilation, this dilation has the diameter greater than the adjacent part of raceway groove, and also comprises the material that is placed in the dilation.This material has before fluid-absorbent the not volume of the path of block fluid.Wherein volume expands immediately so that expand the path of material blocking-up additional fluid in back through raceway groove after the part of this material fluid-absorbent.And this material comprises super absorbent polymer.
The invention provides a kind of fluid means, comprise first reservoir that contains first fluid, second reservoir that contains second fluid, tap drain road, first branch's raceway groove, second branch's raceway groove, first single use pump and second single and use pump.This first branch raceway groove is couple to first reservoir to the tap drain road.And second branch's raceway groove is couple to second reservoir to the tap drain road.Then when the container in first single use pump disconnected, this first single used pump to produce pressure differential with the one or both in the mobile first fluid and second fluid to this first single use pump.And when the container in second single use pump disconnected, second single used pump to produce pressure differential with the one or both in the mobile first fluid and second fluid.
The invention provides a kind of method of controlling fluid, comprise providing a plurality of suction pipes so that the fluid of the scheduled volume of can taking a sample.And each suction pipe comprises raceway groove, and container, and this container produces pressure differential when disconnecting in raceway groove, and this container is made by hard brittle material.This container defines the space in the container, and this space has the gaseous tension less than the gaseous tension of external container, and it breaks container can produce scheduled volume in raceway groove pressure differential, so that the fluid of scheduled volume is drawn to raceway groove.
The invention provides a kind of method of controlling fluid, comprise and make fluid to flow to second area from the first area, wherein, this first area is coupled to second area by the connector with open end and closing end, and this open end is placed in the second area, and this closing end is placed in the first area.The closing end of this first area by connector cuts off with second area, wherein makes fluid to flow to comprise the closing end of disconnect connector, with form pass through connector from the first area to the path of second area.By using the material of volumetric expansion after the fluid-absorbent, the part of adsorbing the fluid of the connector of flowing through, and use through the material that expands and block the additional fluid connector of further flowing through.
The invention provides a kind of method of controlling fluid, comprise fluid is flowed in raceway groove, and this raceway groove comprises the material that volume expands immediately after the part of fluid-absorbent, fluid is flowed comprise flow through material and use described material to come the second portion of fluid-absorbent of the first that makes fluid in raceway groove, thereby make material volume expand.And block the path of additional fluid through raceway groove by using through the material that expands.
The invention provides a kind of method of controlling fluid, comprise and make fluid through raceway groove, this raceway groove comprises first self-closing valve and second self-closing valve.This first self-closing valve and second self-closing valve are spaced apart from each other, and each self-closing valve comprises the fluid adsorption material that volume expands immediately after the part of fluid-absorbent.By using fluid adsorption material in first self-closing valve and second self-closing valve to come the part of fluid-absorbent.The volume of expansion fluid sorbing material further passes through the path of raceway groove with the blocking-up additional fluid, the fluid of maintenance scheduled volume in first self-closing valve of raceway groove and the part between second self-closing valve.
The invention provides a kind of method of controlling fluid, comprise first container that blocking-up made by hard brittle material, make first fluid move to first section of raceway groove from first reservoir in raceway groove, to produce pressure differential.This first container (a) defines the space in first container, this space has the gaseous tension of the gaseous tension that is different from first external container, or (b) comprise first material, this first material is separated with second material before first container disconnects, this first material and this second material through selecting so that produce gas immediately after first material and the interaction of second material.Second container that blocking-up is made by hard brittle material to produce pressure differential in raceway groove, makes the moving and through second section of raceway groove to small part of first fluid.
The invention provides a kind of method of controlling fluid, comprise and operate first single use pump and second single use pump simultaneously, with first's to the first raceway groove of drawing sample fluid and second portion to the second raceway groove of drawing sample fluid.Block first container in first single use pump, to produce pressure differential, make the first of sample fluid move to first raceway groove from reservoir, and block second single and use second container in the pump, make the second portion of sample fluid move to second raceway groove from reservoir to produce pressure differential.Operating the 3rd single simultaneously uses pump and the 4th single to use pump to draw first buffer solution to the first raceway groove and to draw second buffer solution to the second raceway groove.
Description of drawings
Figure 1A and Figure 1B are the synoptic diagram of vacuum pump.
Fig. 2 A and Fig. 2 B are the synoptic diagram of gas pump.
Fig. 3 A and Fig. 3 B are the synoptic diagram of gas pump.
Fig. 4 A is the synoptic diagram of gas pump.
Fig. 4 B is a material list.
Fig. 5 A and Fig. 5 B are the synoptic diagram that breakaway-element is opened valve.
Fig. 6 A, Fig. 6 B, Fig. 7 A, Fig. 7 B and Fig. 8 A to Fig. 8 C are the synoptic diagram of self-closing valve.
Fig. 9 A to Fig. 9 C is the synoptic diagram that valve left by switch.
Figure 10 A to Figure 10 C is for closing the synoptic diagram of switch valve.
Figure 11 A to Figure 11 D is the synoptic diagram of switch switch valve.
Figure 12 is the synoptic diagram of measuring pipet(te).
Figure 13 is the synoptic diagram of measuring pipet(te).
Figure 14 A to Figure 14 C is the synoptic diagram of measuring pipet(te).
Figure 15 A and Figure 15 B are the synoptic diagram of measuring apparatus.
Figure 16 A and Figure 16 B are the synoptic diagram of measuring apparatus.
Figure 17 A to Figure 17 C is the schematic representation of apparatus that is used for the calibrating of two steps.
Figure 18 A to Figure 18 C is the schematic representation of apparatus that is used for the calibrating of two steps.
Figure 19 A to Figure 19 C is the schematic representation of apparatus that is used for the calibrating of three steps.
Figure 20 is the synoptic diagram that is used for the fixed module of multiple analysis quality testing.
Figure 21 A and Figure 21 B show and are used for the take a sample measuring pipet(te) of blood from the patient.
Figure 22 A and Figure 22 B are the schematic representation of apparatus that is used to carry out the calibrating of rapid reaction colorimetric.
Figure 23 A and Figure 23 B are used to take a sample the schematic representation of apparatus of filtered fluid.
Figure 24 A to Figure 24 C is the schematic representation of apparatus that is used to carry out slow colorimetric calibrating.
Figure 25 A to Figure 25 C is the synoptic diagram of vacuum pump.
Figure 26 A and Figure 26 B are the synoptic diagram of vacuum pump.
Figure 27 A to Figure 27 C is the synoptic diagram of self-closing valve.
Figure 28 A and Figure 28 B are the synoptic diagram that breakaway-element is opened valve.
Figure 28 C shows the xsect of the glass capillary with square inside circumference and neighboring.
The combination manufacturing of passing through to use self-closing valve (SLV) and breakaway-element to open valve (BOV) of Figure 29 A and the 29B explanation specific embodiment of the invention is used for blood sample is carried out the device that antibody (Antibody) is examined and determine.
Figure 30 A, 30B and 30C key diagram 29A carry out antibody calibrating installation operation chart to blood sample.
Figure 31 illustrates that the combination manufacturing of passing through to use self-closing valve (SLV) and breakaway-element to open valve (BOV) of another specific embodiment of the present invention is used for blood sample is carried out the device that antibody is examined and determine.
Figure 32 A, 32B are the self-closing valve synoptic diagram with retardation layer.
Shown in Figure 33 A and the 33B, show local coating or comprehensively coating respectively
Figure 34 A and 34B illustrate the embodiment with antibody calibrating installation of time-lag valve proposed by the invention.
Figure 35 A, 35B, 35C and 35D illustrate the antibody calibration method with time-lag valve proposed by the invention.
Figure 36 A and 36B illustrate the centrifugal blood device embodiment with time-lag valve proposed by the invention.
Description of reference numerals
90: vacuum pump 92: gas pump
94: gas pump 96: gas pump
100: container 102: material
104: zone 106: raceway groove
108: direction 110: container
112: zone 114: direction
120: glass capillary 124: raceway groove
128: the second materials of 126: the first materials
130: compound 132: glass capillary
140: breakaway-element is opened valve 142: glass capillary
144: open end 146: closing end
150: the second raceway grooves of 148: the first raceway grooves
162: super absorbent polymer (SAP) 164: raceway groove
166: first area 168: second area
170: self-closing valve 172: enlarged
180: lead 182: pocket regions
190: valve 210 left by switch: the switch switch valve
212: super absorbent polymer (SAP) 214: super absorbent polymer (SAP)
220: measuring pipet(te) 222: measuring pipet(te)
224: suction pipe body 226: spheroid
228: body 230: suction pipe
232: gas pump 234: the suction pipe spheroid
240: measuring pipet(te) 242: spheroid
244: center section 246: the suction pipe body
248: suction pipe body 260: measuring apparatus
262: glass capillary 264: section
266a: the 266b of branch: branch
268a: self-closing valve 268b: self-closing valve
270a: breakaway-element is opened valve 270b: breakaway-element is opened valve
274: fluid 280: measuring apparatus
282: sample pool 284a: kapillary
284b: kapillary 284c: kapillary
286a: self-closing valve 286b: self-closing valve
286c: self-closing valve 288a: vacuum valve
288b: vacuum valve 288c: vacuum valve
290: device 292a: first vacuum pump
292b: the second vacuum pump 292c: the 3rd vacuum pump
294: breakaway-element is opened valve 296: self-closing valve
298: damping fluid 300: sample pool
302: raceway groove 304: raceway groove
306: combination and/or sensing area 308: raceway groove
310: device 314: gas pump
320: device 322: raceway groove
Damping fluid 326 in 324: the second: breakaway-element is opened valve
330: module 332a: chamber
332b: chamber 332c: chamber
334a: vacuum pump 336a: second vacuum pump
338a: the 3rd vacuum pump 340a: the 4th vacuum pump
342a: raceway groove 344a: self-closing valve
346a: self-closing valve 348a: breakaway-element is opened valve
350a: the first damping fluid 352a: self-closing valve
354a: breakaway-element is opened valve 356a: second damping fluid
358a: self-closing valve 360a: breakaway-element is opened valve
362a: the 3rd damping fluid 370: blood
380: device 382: sample pool
384: raceway groove 386: test section
390: device 392: filtration membrane
400: device 402: sample pool
404: gas pump 406: sample fluid
408: band 410: gas pump
412: gas pump 416: the vacuum glass kapillary
418: pipe 420: proofed sleeve
424: closing end 426: open end
428: closing end 430: vacuum pump
432: vacuum pump 434: planar substrates
436: elastic layer 438: fluid channel
440: closing end 442: open end
444: fluid channel 450: lead
460: plastic cement raceway groove 462: the plastic cement raceway groove
464: reactant 466:UV light source
468: detector
500: the device that is used for blood sample is carried out the antibody calibrating
501; Blood sample pond (Blood sample well)
503: dcq buffer liquid pool (Washing buffer well)
505: metering region and sign antibody (Metering zone and labeled antibody) zone
507: diagnosis zone (Diganostic Zone) (antibody array Antibody array)
509: breakaway-element is opened valve 511: discarded object pond (Waste well)
513: self-closing valve (SLV) 550: self-closing valve
552: raceway groove 554: enlarged
556: super absorbent polymer 558: retardation layer
600: antibody calibrating installation 610: corpse or other object for laboratory examination and chemical testing circulation raceway groove
612: the step zone
614: antibody array zone (Antibody Array Area)
616: breakaway-element is opened valve (Broken Open Valve)
620: labelled antibody groove (Labeled Antibody Well)
630: sample cell (Sample Well)
640: abacus (Washing Buffer Well)
622: 632: the second time-lag valves of very first time retardation valve
625: labelled antibody 635: blood sample
660: setup time the retardation valve separation of whole blood device 662: the separation of whole blood deferent
664: time-lag valve 666: rotating shaft
668: centrifugal force direction (Centrifugal Direction)
661: whole blood sample (Whole Blood Sample)
663: blood cell district (Blood Cell Area)
665: blood plasma district (Blood Plasma Area)
Embodiment
A kind of fluid means that is used to carry out calibrating can comprise the Control Component such as vacuum pump, gas pump, " breakaway-element is opened valve (Broken open valves) " and " self-closing valve (self-close valves) ", and its fluid that is used for controlling fluid means flows.Can use vacuum pump along the fluid in the specific direction traction raceway groove, but and the using gases pump come to promote fluid in the raceway groove along specific direction.Can use breakaway-element to open valve and connect two isolated areas controlling by the user, and can use self-closing valve to seal raceway groove automatically through later at fluid.Vacuum pump, gas pump, breakaway-element are opened valve and self-closing valve and can be fabricated to for a short time, make fluid means can be fabricated to little and for portable.
In the following description, can at first introduce indivedual Control Components, and how be used for controlling the description of modular unit of the fluid of fluid means subsequently for Control Component with construction through combination.To describe subsequently and how use fluid means to carry out biological calibrating.
Referring to Figure 1A, can come construction vacuum pump 90 by container 100 being placed in the raceway groove 106 (or chamber) that defines by material 102.Container 100 closed regions 104, zone 104 are for vacuum or have a low gaseous tension of comparing with the gaseous tension in the raceway groove 106.
Referring to Figure 1B, container 100 can be (for example) glass capillary, and it disconnects after applying external force immediately.When container 100 disconnected, the gas in the raceway groove 106 flowed in the vacuum area 104, thereby reduced the pressure in the zone 106.Producing in this way can be along direction 108 draw fluid so that its attractive force towards zone 106.
Figure 25 A to Figure 25 C shows the example that uses the vacuum pump that is placed in the glass capillary in the proofed sleeve.Figure 25 A shows the xsect of gas pump 410, and gas pump 410 has the vacuum glass kapillary 416 that is placed in the proofed sleeve 418, wherein manages 418 and has closing end 424 and open end 426.Figure 25 B shows the xsect of gas pump 412, and gas pump 412 is similar to gas pump 410, has the proofed sleeve 420 of two open ends except having one.Figure 25 C shows the gas pump 412 that is connected to two proofed sleeves 428, and wherein proofed sleeve 420 has than the internal diameter (to hold glass capillary 416) greater than proofed sleeve 428.
Figure 26 A and Figure 26 B show the example that uses the vacuum pump that is placed in the glass capillary in the planar flows bulk channel.Figure 26 A shows the xsect of vacuum pump 430, and vacuum pump 430 has the vacuum glass kapillary 416 that is placed in the fluid channel 438 that is defined by planar substrates 434.Fluid channel 438 has closing end 440 and open end 442.Planar substrates 434 is made by rigid material.Elastic layer 436 is embedded in the position of adjacent capillaries 416 in the substrate 434, makes to allow the user to apply external force to disconnect kapillary 416 via elastic layer.
Figure 26 B shows the xsect of vacuum pump 432, and vacuum pump 432 is similar to vacuum pump 430, except fluid channel 438 is connected to two fluid channel 444 with smaller cross-sectional area.
Can form first closure with melten glass by a heating glass end capillaceous brings in and makes the vacuum glass kapillary.Use vacuum pump to go out air in the glass capillary via the open end pumping.At distance first closing end one distance heating glass kapillary.The heat soften glass can be pinched and contract or reverse through softening glass to form second closing end.
Referring to Fig. 2 A, can come construction gas pump 92 by container 110 being placed in the raceway groove 106 (or chamber) that defines by material 102.The gaseous tension that container 110 closed regions 112, zone 112 have in the raceway groove 106 with container 110 outsides is compared higher gaseous tension.
Referring to Fig. 2 B, container 110 can be (for example) glass capillary, and it disconnects after applying external force immediately.When container 110 disconnects,, thereby increased pressure in the zone 106 originally at container 110 gas inside flow containers 110.Producing in this way can be along direction 114 propelling fluids so that its power away from zone 106.
In this describes, will use term " vacuum pump " to make a general reference a kind of device that produces tractive force, it can be in order to towards installing draw fluid; And will use term " gas pump " to make a general reference a kind of device that produces expulsive force, it can be in order to propelling fluid so that it be away from device.
Existence is in order to the alternative of construction gas pump.For example, referring to Fig. 3 A, can make gas pump 94 by the partially filled glass capillary 120 that first material 126 arranged is placed in the raceway groove 124 (or chamber) that contains second material 128.Select first material 126 and second material 128, make when material 126 and material 128 are mixed with each other, will interact and produce one or more gas.For example, first material 126 can be sodium carbonate (Na
2CO
3) and/or sodium bicarbonate (NaHCO
3), and second material 128 can be acetate (CH
2COOH).
Referring to Fig. 3 B, when applying external force with disconnection glass capillary 120, first material 126 and second material 128 interact and produce gas.In this example, gas is carbon dioxide (CO
2).The chemical reaction that takes place is:
Na
2CO
3+2CH
2COOH→2NaCOOCH
2+H
2O+CO
2
NaHCO
3+CH
2COOH→NaCOOCH
2+H
2O+CO
2
Carbon dioxide increases the pressure in the raceway groove 124, can be in order to the power of propelling fluid away from the kapillary 120 that disconnects thereby produce.
Referring to Fig. 4 A, can make gas pump 96 by following steps: with compound 130 be placed in the glass capillary 132, sealed hair tubule 132, heated capillary 132, cooling kapillary 132 and kapillary 132 is placed in the raceway groove 106 (or chamber).Compound 130 is chosen as the material that after heating, produces gas.When heating and cooling kapillary 132, the gas that is produced by compound 130 increases kapillary 132 gas inside pressure (comparing with the gaseous tension of kapillary 132 outsides).
The example of compound 130 comprises sodium bicarbonate (NaHCO
3) and lime carbonate (CaCO
3).Produce carbon dioxide during these compound heating:
NaHCO
3→NaOH+CO
2
CaCO
3→CaO+CO
2
Also can use from solid state change and (for example, become CO as the sublimator material of gaseous state
2Dry ice).List in the material list of Fig. 4 B that when heating produces the other materials of gas or as NaN
3Heating produces nitrogen (2NaN
3→ 2Na+3N
2).
Referring to Fig. 5 A, can make breakaway-element between first raceway groove 148 and second raceway groove 150 and open valve 140 by glass capillary 142 is placed in.Glass capillary 142 has the open end 144 that is positioned in first raceway groove 148, and is positioned the closing end 146 in second raceway groove 150.When glass capillary was intact, fluid can't flow between first raceway groove 148 and second raceway groove 150.This is called " closing " state that breakaway-element is opened valve.
Referring to Fig. 5 B, when applying external force with disconnection glass capillary 142, the path 152 that connects raceway groove 148 and raceway groove 150 has formed.This is called " opening " state that breakaway-element is opened valve.Breakaway-element is opened valve 140 and be can be used for allowing two kinds of fluids (or a kind of fluid and a kind of solid) prima facies to separate, and interacts in the moment by user's control subsequently.
Figure 28 A and Figure 28 B displaying use breakaway-element to open the example that valve comes the low-cost device of construction, and low-cost device is used for carrying out the calibrating of wherein using ultraviolet (UV) optical radiation fluid.Glass capillary 142 connects two plastic cement raceway grooves 460 and 462.Initially, reactant 464 is contained in the first plastic cement raceway groove 462.After glass capillary 142 disconnects, reactant 464 glass capillary 142 to the second plastic cement raceway grooves 460 of flowing through immediately.Shown in Figure 28 B, when reactant 464 is flowed through glass capillary 142, the 466 radioreaction agent 464 of UV light source.Detector 468 detectings are by the UV light of reactant 464.The spectrum of the UV light that detector 468 is detected can be used for judging the compound in the reactant 464.
Figure 28 C shows the xsect of the glass capillary with square inside circumference and neighboring.Square inside circumference and neighboring allow UV light edge to pass through glass capillary perpendicular to the direction on the surface of glass capillary.Have circular cross section with kapillary and compare, this mode that kapillary has a square cross section allows more UV light to reach fluid in the glass capillary, and wherein circular cross section may cause that incident UV light is along away from the direction reflection of fluid or be redirected.
Referring to Fig. 6 A and Fig. 6 B, can make self-closing valve 160 in the raceway groove 164 by super absorbent polymer (Superabsorbentpolymer, beneath abbreviation " SAP ") 162 is placed in.Initially, SAP 162 has smaller size smaller and allows between the first area 166 of fluid in raceway groove 164 and the second area 168 mobile (Fig. 6 A).This is called self-closing valve and " opens " state.When fluid was flowed through SAP 162, the part of SAP fluid-absorbent and volumetric expansion, thereby blocking-up raceway groove 164 (Fig. 6 B) stoped fluid further to flow between first area 166 and second area 168 thus.This is called self-closing valve and " closes " state.
Super absorbent polymer is adsorbable and keep the large volume of water or other aqueous solution.In some instances, SAP can be by making through the starch of chemical modification and cellulose and other polymkeric substance, such as, high-hydrophilic and to glassware for drinking water poly-(vinyl alcohol) PVA of high-affinity, poly-(ethylene oxide) PEO arranged.In some instances, super absorbent polymer can be made by part neutralization and lightly crosslinked poly-(acrylic acid), and part neutralization and lightly crosslinked poly-(acrylic acid) have the ratio of good usefulness relative cost.Polymkeric substance can manufacture has low solid content, subsequently polymkeric substance drying and grinding is the white particulate solid.In water, white solid is expanded to can comprise the rubber gels of percentage by weight up to 99% water in some cases.
Referring to Fig. 7 A, self-closing valve 170 can comprise raceway groove 164, and raceway groove 164 has enlarged 172 to hold super absorbent polymer 162, makes super absorbent polymer 162 flowing of limit fluid not before expansion.In order to make self-closing valve 170, adhesive can be coated to the inwall of enlarged 172, be pushed into subsequently in the raceway groove 164 with the SAP 162 of powder morphology, make the inwall of SAP 162 powder adherence to enlarged 172 places.
Referring to Fig. 7 B, when fluid is flowed through super absorbent polymer 162, the part and the volumetric expansion of super absorbent polymer 162 fluid-absorbent, thereby blocking-up raceway groove 164, the polymkeric substance 162 that stops fluid further to flow through and expand thus.
Referring to Fig. 8 A and Fig. 8 B, super absorbent polymer 162 can be attached to lead 180, is placed in subsequently in the raceway groove 164.Raceway groove 164 can have pocket regions 182, is coated with adhesive in the pocket regions 182 and defines the position in advance so that lead 180 is anchored on.
Referring to Fig. 8 C, when fluid is flowed through super absorbent polymer 162, the part and the volumetric expansion of polymkeric substance 162 fluid-absorbent, thereby blocking-up raceway groove 164, the polymkeric substance 162 that stops fluid further to flow through and expand thus.
Can be by being coated with lead with SAP, will being placed in raceway groove or the pipe and making self-closing valve through the lead of coating subsequently.Can be by being coated with planar substrates with SAP, will being placed in the planar channeling in the planar flows body device through the substrate of coating and making the self-closing valve that is used for the planar flows body device subsequently.
Referring to Fig. 9 A to Fig. 9 C, can make switch by the SAP 162 that uses glass capillary 142 and be positioned kapillary 142 outsides and adjacent capillaries 142 and drive valve 190.Kapillary 142 all is positioned to have in the raceway groove 164 of first area 166 and second area 168 with SAP162.Use glass capillary 142 and SAP to be similar to the combination of using breakaway-element to open valve and self-closing valve.Switch open valve 190 make the user can by allow, blocking-up subsequently and allow fluid to control fluid the flowing of ad-hoc location in the raceway groove of flowing through subsequently through ad-hoc location.
Referring to Fig. 9 A, initial, SAP 162 has smaller size smaller and does not block raceway groove, thereby allows fluid to flow between first area 166 and second area 168.
Referring to Fig. 9 B, when fluid through out-of-date, the part of fluid is adsorbed by SAP 162, thereby makes the SAP162 volume increase, thus block fluid further mobile between first area 166 and second area 168.
Referring to Fig. 9 C, when applying external force, produce path 152 and between first area 166 and second area 168, flow to allow fluid with disconnection glass capillary 142.
Referring to Figure 10 A to Figure 10 C, can make by the SAP 162 that uses glass capillary 142 and be positioned kapillary 142 inside and close switch valve 200.Kapillary 142 has open end 144 and closing end 146.Open end 144 is positioned in first raceway groove 148, and closing end 146 is positioned in second raceway groove 150.Glass capillary 142 and SAP 162 carry out and are similar to the function that breakaway-element is opened the combination of valve and self-closing valve.Close switch valve 200 make the user can be by blocking-up, subsequently allow and subsequently block fluid control fluid the flowing of ad-hoc location in the raceway groove of flowing through through ad-hoc location.
Referring to Figure 10 A, when glass capillary 142 was intact, first raceway groove 148 was not connected with second raceway groove 150.
Referring to Figure 10 B, when applying external force, form path 152, thereby allow fluid between raceway groove 148 and 150, to flow with disconnection glass capillary 142.SAP 162 initially has smaller size smaller and non-block fluid flowing in path 152.
Referring to Figure 10 C, when fluid was flowed through path 152, the part of fluid was adsorbed by SAP 162, thereby made the SAP volume increase and blocking-up path 152, stoped the fluid path 152 of further flowing through thus.
Referring to Figure 11 A to Figure 11 D, can be by using glass capillary 142, be positioned the SAP 212 of kapillary 142 inside, and the SAP 214 that is positioned kapillary 142 outsides makes switch switch valve (on-off-on-off valve).Glass capillary 142, SAP 212, and SAP 214 is placed in the raceway groove 164.Glass capillary 142, SAP 212, and SAP 214 execution are similar to the function that breakaway-element is opened the combination of valve and two self-closing valves.Switch switch valve 210 make the user can by allow, blocking-up subsequently, subsequently allow and subsequently block fluid control fluid the flowing of ad-hoc location in the raceway groove of flowing through through ad-hoc location.
Referring to Figure 11 A, initial, SAP 214 has smaller size smaller and allows fluid in mobile between the first area 166 of raceway groove 164 and the second area 168.
Referring to Figure 11 B, when fluid through out-of-date, the part of fluid is adsorbed by SAP 214, thereby makes the SAP214 volume increase, thus block fluid further mobile between first area 166 and second area 168.
Referring to Figure 11 C, when applying external force, form path 152 and between first area 166 and second area 168, flow to allow fluid with disconnection glass capillary 142.
Referring to Figure 11 D, when fluid was flowed through SAP 212, the part of fluid was adsorbed by SAP 212, thereby made SAP 212 volumes increase and blocking-up path 152, stoped the fluid path 152 of further flowing through thus.
Referring to Figure 12, can come construction to be used to draw the measuring pipet(te) (Metering Pipette) 220 of the fluid of scheduled volume by the vacuum pump 222 that use is coupled to suction pipe body 224.Vacuum pump 222 comprises the vacuum glass kapillary 100 that is placed in the suction pipe spheroid 226.In order to use measuring pipet(te) 220, disconnect glass capillary 100 and fluid is drawn attractive force to the suction pipe body 224 to produce.
When making a collection of measuring pipet(te) 220, the size of spheroid 226 and glass capillary 100 can be made into identical.Spheroid 226 and glass capillary 100 to push spheroid 226 as the user when disconnecting glass capillary 100, cause that it is that cardinal principle is identical that glass capillary 100 disconnects the needed deflection of bestowing spheroid 226 through design for all measuring pipet(te)s 220 for.In this way, the user can use measuring pipet(te) 220 to draw the fluid of scheduled volume fast under need not to monitor the situation of the fluid content in the stem shape thing 224.
For example, referring to Figure 21 A and Figure 21 B, can use measuring pipet(te) 220 fast from the take a sample blood 370 of scheduled volume of patient.
Referring to Figure 13, another example of measuring pipet(te) 230 comprises vacuum pump 222 and gas pump 232.Vacuum pump 222 is similar to vacuum pump shown in Figure 12.Gas pump 232 comprises glass capillary 120, and glass capillary 120 is filled with Na
2CO
3And be placed in and contain CH
2In the suction pipe spheroid 234 of COOH.When glass capillary 120 disconnects, Na
2CO
3With CH
2COOH interacts to produce CO
2Thereby, increase the gaseous tension in the spheroid 234.Vacuum pump 222 allows the user to draw the fluid of scheduled volume fast to suction pipe 230.Gas pump 232 permission users execute fluid and are assigned to outside the suction pipe 230.
The advantage of using gases pump 232 is: work as Na
2CO
3With CH
2Reaction between the COOH produces CO
2During gas, can during controlled a period of time, execute the fluid in the pipe arrangement body 228.In this way, the user need not the careful outflow of monitoring fluid when executing dispense fluid.
Referring to Figure 14 A, another example of measuring pipet(te) 240 comprises spheroid 242, center section 244 and suction pipe body 246.Center section 244 is by the deformable material construction.Switch leaves valve 248 and is positioned in the center section 244.The SAP 162 that valve 248 comprises glass capillary 142 and is positioned kapillary 142 outsides left by switch, and this is similar to the device shown in Fig. 9 A to Fig. 9 C.
Referring to Figure 14 A, in order to use suction pipe 240, user's extruding and discharge spheroid 242 with draw fluid to body 246 and center section 244.
Referring to Figure 14 B, when fluid reached center section 244 and beginning and contacts with SAP 248, the part of fluid was adsorbed by SAP 248, thereby made the fluid passage of opposite side of SAP 248 volumetric expansions and blocking-up SAP 248.In this way, the fluid of scheduled volume is drawn to suction pipe 240.
Referring to Figure 14 C, for self-straw 240 is executed dispense fluid, the user pushes center section 244 (being made by deformable material) with disconnection glass capillary 142, thereby forms the path through the kapillary 142 that disconnects.The user pushes spheroid 242 subsequently and flows out suction pipe 240 to force fluid.
When making a collection of suction pipe 240, body 246 and center section 244 are of a size of identical, and the position that the switches in the center section 244 are driven valve 248 is identical, makes the user can use under the situation of the content liquid of suction pipe 240 in need not accurate monitoring suction pipe 240 and draws the fluid of same amount substantially fast.
Referring to Figure 15 A, the measuring apparatus 260 that is used to collect the fluid of scheduled volume comprises glass capillary 262, and glass capillary 262 has two 266a of branch and 266b, two self-closing valve 268a and 268b, and two breakaway-elements are opened valve 270a and 270b.Among self-closing valve 268a and the 268b each has the SAP that expands immediately after the fluid-absorbent.Initially, self-closing valve 268a and 268b are in open mode, and breakaway-element is opened valve 270a and 270b is in closed condition.Self-closing valve 268a and 268b can be similar to the self-closing valve shown in Fig. 6 A to Fig. 8 C.Breakaway-element is opened valve 270a and 270b and can be similar to the breakaway-element shown in Fig. 5 A and Fig. 5 B and open valve.
In operation, owing to capillary force, fluid 274 is drawn to kapillary 262, and flow through self-closing valve 268a and 268b.Referring to Figure 15 B, when fluid 274 is flowed through self-closing valve 268a and 268b, the part of fluid 274 is adsorbed by the SAP among self-closing valve 268a and the 268b, thereby makes self-closing valve 268a and 268b be changed to closed condition, and block fluid 274 further flows thus.Cause fluid 274 to occupy section 264 between self-closing valve 268a capillaceous and the 268b in this way.
Change into open mode and apply attractive force or expulsive force is come mobile fluid 274 by breakaway-element being opened valve 270a and 270b self closing state, fluid 274 can move to other positions from section 264 through 266a of branch or 266b.
The advantage of measuring apparatus 260 is: measuring apparatus 260 can be at the fluid that need not quick sampling predetermined under the careful situation about monitoring of user.Because kapillary has minor diameter, so measuring apparatus 260 can be used for accurately taking a sample small volume of fluid.
Referring to Figure 16 A, can comprise three kapillary 284a, 284b and 284c from the measuring apparatus 280 that sample pool 282 obtains the fluids of three kinds of different amounts.Each kapillary at one end has self-closing valve (for example, 286a, 286b or 286c) and the other end has vacuum valve (for example, 288a, 288b or 288c).Each vacuum pump has the vacuum glass kapillary.Initially, self-closing valve is in open mode.
Referring to Figure 16 B, when the user disconnects vacuum glass kapillary among the vacuum pump 288a, produce attractive force and draw predetermined amount of liquid to kapillary 284a.When fluid process self-closing valve 286a, the SAP among the self-closing valve 286a expands, thereby makes self-closing valve 286a enter closed condition, stops fluid to be moved further thus and process self-closing valve 286a.Similar, by the vacuum kapillary among blocking-up vacuum pump 288b and the 288c, the fluid of scheduled volume can be drawn to kapillary 284b and 284c.The Fluid Volume of being drawn to the kapillary 284a to 284c judged by the volume capillaceous among the vacuum pump 288a to 288c, and the Fluid Volume of being drawn to the kapillary 284a to 284c can be identical or different.
Referring to Figure 17 A, device 290, the two steps calibrating that can be used for the calibrating of two steps by the combination manufacturing of using vacuum pump, breakaway-element to open valve, self-closing valve requires cleaning with damping fluid behind the binding reagents fast.One end of raceway groove 302 is coupled to sample pool 300 through self-closing valve 296, and the other end of raceway groove 302 is coupled to the first vacuum pump 292a.Raceway groove 302 is connected to raceway groove 308, and raceway groove 308 is opened valve 294 through breakaway-element and is coupled to damping fluid 298.Raceway groove 302 also is connected to raceway groove 304, and raceway groove 304 is coupled to the second vacuum pump 292b and the 3rd vacuum pump 292c.Raceway groove 304 comprise in conjunction with and/or sensing area 306, in conjunction with and/or sensing area 306 comprise and be used for the combination or the reagent of sensing sample 300 compounds.
With a mode operating means 290 so that towards in conjunction with and sensing area 306 draw samples 300 so that reaction takes place, subsequently towards in conjunction with and sensing area 306 draw damping fluids 298 with clean in conjunction with and sensing area.
Referring to Figure 17 B, start vacuum pump 292a and draw to the attractive force of the part of the raceway groove 302 between vacuum pump 292a and the self-closing valve 296 to produce towards vacuum pump 292a absorption sample 300 and with sample 300.When sample 300 was flowed through self-closing valve 296, the part of sample was adsorbed by the SAP in the self-closing valve 296, thereby makes self-closing valve 296 enter closed condition.
Referring to Figure 17 C, start breakaway-element and open valve 294 so that valve 294 is changed into open mode.Start vacuum pump 292b to produce the attractive force of drawing sample 300 and damping fluid 298 towards vacuum pump 292b.Vacuum pump 292a and 292b through design so that after starting pump, sample 300 will stop in conjunction with and sensing area 306 places.Over time, start vacuum pump 292c with mobile sample 300 to district 306, and make damping fluid 298 flow through district 306 and cleaning area 306.
Above-mentioned example provides incubation time (incubation time), and the compound in the sample 300 reacts with the reagent of distinguishing in 306 before damping fluid 290 cleaning combinations and sensing area 306 to allow.If the reaction at 306 places, district is unnecessary for fast and incubation time, then vacuum pump 292b can make big and can omit vacuum pump 292c.When starting vacuum pump 292b, sample flow through rapidly in conjunction with and sensing area 306, subsequently by damping fluid 298 clean in conjunction with and sensing areas 306.
Referring to Figure 18 A, device 310, the two steps calibrating that can be used for the calibrating of two steps by the combination manufacturing of using vacuum pump, breakaway-element to open valve, self-closing valve and gas pump requires slowly cleaning with damping fluid behind the binding reagents.The device 310 that is similar to device 290 has raceway groove 302, and raceway groove 302 is connected to two raceway grooves 304 and 308.Raceway groove 302 is coupled to sample 300 through self-closing valve 296.Raceway groove 308 is opened valve 294 through breakaway-element and is coupled to damping fluid 298.Raceway groove 304 comprises combination and sensing area 306.One end of raceway groove 304 is coupled to breakaway-element and opens valve 312.Gas pump 314 is coupled to damping fluid 298.
Referring to Figure 18 B,, start vacuum pump 292a to draw sample 300 to raceway groove in order to carry out the calibrating of two steps.Enter closed condition at sample flow valve 296 behind self-closing valve 296.
Referring to Figure 18 C, start breakaway-element and open valve 294 and 312 so that valve is changed into open mode.Start gas pump 314 during a period of time, producing gas, thus promote sample 300 and damping fluid 298 through in conjunction with and sensing areas 306.Because gas pump 314 is producing gas (reaction between the compound of generation gas expends a certain amount of time and finishes) during a period of time, sample 300 can slowly pass through combination and sensing area 306, thereby association reaction slowly takes place.
Referring to Figure 19 A, can require cleaning with two kinds of damping fluids behind the binding reagents fast by adding device 320, the three steps calibrating that the structure manufacturing shown in second damping fluid 324 and raceway groove 322 to Figure 17 A is used for three steps calibratings.Suddenly examine and determine in order to carry out multistep, start vacuum pump 292a so that sample 300 flows to raceway groove 302.When sample 300 was flowed through self-closing valve 296, valve 296 was changed into closed condition.
Referring to Figure 19 B, start breakaway-element and open valve 294, and start vacuum pump 292b so that draw the sample 300 and first damping fluids 298 towards combination and sensing area 306 so that it changes into open mode.
Referring to Figure 19 C, start breakaway-element and open valve 326, and start vacuum pump 292c so that draw sample 300, first damping fluid 298 and second damping fluids 324 towards combination and sensing area 306 so that it changes into open mode.In this way, the reaction at available two kinds of 306 places, different buffer solution for cleaning district.
Can be by coupling extra damping fluid or sample and adding a corresponding number vacuum pump to the end of raceway groove 304 and come construction to be used for the device of the calibrating of the step more than requirement three steps.
Referring to Figure 20, but construction module 330 is carried out the multiple analysis quality testing calmly.Module comprises the sample pool 282 that is used for fixing sample 300 and three chamber 332a, 332b and 332c, each chamber contain be useful in conjunction with and sensing sample 300 in the analyte of compound.Below describe in order to carry out the assembly of the calibrating relevant with first analyte among the chamber 332a.
In order to carry out calibrating, start vacuum pump 334a to draw sample 300, so that allow compound and analyte 332a in the sample 300 to react towards chamber 332a.After self-closing valve 344a, valve 344a changes into closed condition in a certain amount of sample flow.Open the valve 348a (valve is changed into open mode) and the second vacuum pump 336a by starting breakaway-element, the first damping fluid 350a washed chamber 332a.After a certain amount of first damping fluid 350a flowed through self-closing valve 346a, valve 346a changed into closed condition.
In this similar mode, open valve 360a (valve is changed into open mode) and the 4th vacuum pump 340a by starting breakaway-element, the 3rd damping fluid 362a washed chamber 332a.After a certain amount of the 3rd damping fluid 362a flowed through self-closing valve 358a, valve 358a changed into closed condition.
Can be similar to the mode of carrying out the calibrating relevant with first analyte among the chamber 332a carry out with chamber 332b and 332c in the calibrating of being correlated with of the second and the 3rd analyte.Can carry out simultaneously with chamber 332a, 332b and 332c in first, second and the relevant calibrating of the 3rd analyte.
Below be the vacuum pump of the biological calibrating of execution and the application of gas pump.
Figure 22 A and Figure 22 B show the device 380 that is used to carry out the calibrating of rapid reaction colorimetric.Device 380 comprises raceway groove 384, and an end of raceway groove 384 is coupled to sample pool 382 and the other end is coupled to vacuum pump 90.But the fluid of sample pool 382 fixings such as blood or urine.Raceway groove 384 comprises test section 386, and test section 386 has and detects the p-wire that changes color behind a certain compound immediately.When starting, can draw by vacuum pump 90 fluid in the sample pool 382 fast through test section 386.By the color of read test line, the user can judge fast in the fluid and to have or not exist a certain compound.
Figure 23 A and Figure 23 B show the device 390 of the filtered fluid that is used to take a sample.Device 390 comprises raceway groove 384, and an end of raceway groove 384 is coupled to sample pool 382 and the other end is coupled to vacuum pump 90.Filtration membrane 392 is placed in the sample pool 382.Can draw fluid (for example, blood) in the sample pool 382 when vacuum pump 90 starts fast through filtration membrane 392, thereby produce the filtered fluid of being drawn to the raceway groove 384 (blood plasma).
Figure 24 A to Figure 24 C shows the device 400 that is used for colorimetric calibrating slowly.Device 400 comprises sample pool 402, and sample pool 402 is coupled between gas pump 404 and the raceway groove 384.Raceway groove 384 has test section 386, and test section 386 has and detects the p-wire that changes color behind a certain compound immediately.For operative installations 400, sample fluid 406 is placed in the sample pool 402.The opening of band 408 sealing sample pools.Start gas pump 404 and promote the gas of sample fluid 406 through test section 386 to produce.Because gas pump 404 produces gas during a period of time, thus sample fluid 406 during a period of time, advance through the test section, thereby allow to carry out slowly the colorimetric calibrating.
A specific embodiment of the present invention then please refer to Figure 29 A and 29B, can be used for blood sample is carried out the device 500 of antibody (Antibody) calibrating by the combination manufacturing of using self-closing valve (SLV) and breakaway-element to open valve (BOV).This device 500 comprises that blood sample pond (Blood samplewell) 501, dcq buffer liquid pool (Washing buffer well) 503, metering region and sign antibody (Metering zone and labeled antibody) zone 505, diagnosis zone (DiganosticZone) (antibody array Antibody array) 507, breakaway-element open valve (BOV) 509, discarded object pond (Wastewell) 511 and self-closing valve (SLV) 513.This metering region has a raceway groove with sign antibody regions 505 and is communicated with blood sample pond 501 and dcq buffer liquid pool 503, and self-closing valve (SLV) 513 then is between this raceway groove.Diagnosis zone 507 then is to have another raceway groove, and an end is connected to metering region and the center that indicates antibody regions 505, and the other end is then opened valve (BOV) 509 by breakaway-element and is connected to discarded object pond (Waste well) 511.
The method that 500 pairs of blood samples of this device carry out antibody (Antibody) calibrating please refer to shown in Figure 30 A, 30B and the 30C.At first, blood sample 502 is written in the blood sample pond 501, then, use the self-closing valve (SLV) 513 that is proposed in the previous embodiment of the present invention, utilize capillary force (Capillaryforce) blood to be sucked in the raceway groove of this metering region and sign antibody regions 505 502a shown in Figure 30 A.Then, be written into dcq buffer liquid in dcq buffer liquid pool 503, and this moment, dcq buffer liquid can flow into also in the raceway groove of metering region and sign antibody regions 505, as the 504a of Figure 30 B, and this moment, blood can be squeezed in the diagnosis zone 507, as the 502b of figure.
Then, please refer to Figure 30 C, after breakaway-element opened valve (BOV) 509 and disconnect, then blood is further sucked and process diagnosis zone 507,502c as shown in the figure, this moment, the antibody array (Antibody array) in this diagnosis zone 507 will combine with the antigen (Antigent) in the blood, and dcq buffer liquid then will be flushed to discarded object pond 511 less than the blood of effect.
Another specific embodiment of the present invention, and referring to shown in Figure 31.Can be used for blood sample is carried out the device of antibody (Antibody) calibrating by the combination manufacturing of using self-closing valve (SLV) and breakaway-element to open valve (BOV).And comprising blood sample pond, dcq buffer liquid pool, diagnosis zone (including antibody array), breakaway-element, this device opens valve (BOV), discarded object pond and self-closing valve.This blood sample pond then comprises and has the film that removes haemocyte (Blood cell removal), can utilize blood sample can filter out haemocyte when sucking.
This diagnosis zone has a raceway groove and is communicated with blood sample pond and dcq buffer liquid pool, and the other end is then opened valve (BOV) by breakaway-element and is connected to the discarded object pond.Self-closing valve (SLV) then is to be positioned at the blood sample pond to enter before this raceway groove, and may command flows into the blood flow volume in diagnosis zone, and prevents that backflow of blood is to the blood sample pond.
After breakaway-element is opened valve (BOV) disconnection, behind the blood elder generation process membrane filtration of removable haemocyte, further suck in the diagnosis zone, this moment, the antibody array (Antibodyarray) in the diagnosis zone will combine with the antigen (Antigent) in the blood, and dcq buffer liquid then will be flushed in the discarded object pond less than the blood of effect.
Above-mentioned super absorbent polymer (Superabsorbent polymer, " SAP ") is adsorbable and keep the large volume of water or other aqueous solution.In some instances, SAP can be by making through the starch of chemical modification and cellulose and other polymkeric substance, such as, high-hydrophilic and to glassware for drinking water poly-(vinyl alcohol) PVA of high-affinity, poly-(ethylene oxide) PEO arranged.In some instances, super absorbent polymer can be made by part neutralization and lightly crosslinked poly-(acrylic acid), and part neutralization and lightly crosslinked poly-(acrylic acid) have the ratio of good usefulness relative cost.
And in self-closing valve, use super absorbent polymer, because about several seconds of the gel time of polymkeric substance (Gelling Time) is applicable to vacuum pump collocation application.When the application that capillary force (Capillary Force) and centrifugal force (Centrifugal Force) drive, therefore rate of flow of fluid only has the fluid of prevention to flow less than the gel time of SAP, can't allow fluid flowing in raceway groove.Therefore, the application of self-closing valve proposed by the invention, can be by the design of joining day retardation layer, delay " closing " time of SAP self-closing valve, in addition, this retardation layer can be selected not influence the material that detects subject matter and get final product, and can reach the application that is suitable for slug flow speed, is called time-lag valve (Time Lag Valve) at this.
In this embodiment, utilize soluble material to be configured in the self-closing valve raceway groove, between super absorbent polymer SAP and fluid, when detecting flow of solution through this raceway groove, SAP absorption and keep the speed of the large volume of water or other aqueous solution can be subjected to the retardation of soluble material and the speed of expansion gel eases up, this can reach the requirement of slug flow speed.
Referring to Figure 32 A, self-closing valve 550 can comprise raceway groove 552, and raceway groove 552 has enlarged 554 to hold super absorbent polymer 556, makes super absorbent polymer 556 flowing of limit fluid not before expansion.In order to make self-closing valve 550, adhesive can be coated to the inwall of enlarged 554, be pushed into subsequently in the raceway groove 552 with the SAP 556 of powder morphology, make the inwall of SAP 556 powder adherence to enlarged 554 places.Utilize the made retardation layer 558 of soluble material then can place raceway groove 552 in the raceway groove of the fluid flow of enlarged 554.Referring to Figure 32 B, when fluid in when circulation, after retardation layer 558 dissolving by the time, super absorbent polymer 556 just begins the part of fluid-absorbent and volumetric expansion, thus blocking-up raceway groove 552 stops further the flow through polymkeric substance 556 of expansion of fluid thus.
The selection of this retardation layer material, based on soluble material, in one embodiment, it is main can selecting water-soluble material, for example sucrose piece (sugar cube) or sucrose sheet (sugar sheet), thickness is between between 0.01～2mm, and pad is overlying on runner and super absorbent polymer (SAP) storeroom.When water-soluble liquid is flowed through runner, contact with sucrose earlier and carry out dissolution, stop the absorption moisture of SAP, while allow the SAP imbibition when sucrose dissolves the space of being vacateed.After the sugar dissolving finished, SAP can block runner fully, finishes the start of " closing ".This lag time is along with the thickness of sucrose increases and prolongs, and this can design according to demand.In addition, the selection of different retardation layer materials also can have different lag time effects.
In other embodiment, the raceway groove 552 of self-closing valve 550 can not need enlarged 554, and the substitute is the inwall that super absorbent polymer 556 powder can be adhered to raceway groove 552 places, and these super absorbent polymer 556 powder can be with the SAP material fixing after, carry out the immersion type coating (dip-coating) of fusion syrup, drum-type coating (Roll Coating) or rotary coating technologies such as (SpinCoating), the retardation layer material is blended in super absorbent polymer 556 powder, for example shown in Figure 33 A and the 33B, show local coating or comprehensively coating respectively.And SAP material absorption this moment and keep the speed retardation to some extent of the large volume of water or other aqueous solution, this also reaches this purpose.
Above-mentioned time-lag valve design can be used in a plurality of examples, and for example Figure 34 A and 34B paint the device of formula antibody (Antibody) calibrating.In this antibody calibrating installation 600, comprise corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 and the labelled antibody groove (Labeled Antibody Well) 620 that is connected, sample cell (Sample Well) 630, abacus (Washing Buffer Well) 640.And have two above-mentioned time- lag valves 622 and 632 respectively between this corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 and labelled antibody groove 620 and the sample cell 630.Comprise on the corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 that step zone 612, antibody array zone (Antibody ArrayArea) 614 open valve (Broken Open Valve) 616 with breakaway-element.
This step zone 612 mainly is the design that cooperates time-lag valve 622 and 632.The runner caliber of corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 along the design of the step shown in the sectional view AA ', by big runner caliber 611 then change into next runner caliber 613, then transfers less runner caliber 615 to as Figure 34 B.The design of this kind convergent current road caliber can effectively increase the size of downstream capillary force.With step (step) design, cooperate the use of time- lag valve 622 and 632, when liquid bath flows into the particular conduit interval, also can reach the function that liquid is decided volume.
Use the antibody calibration method as the antibody calibrating installation 600 of Figure 34 A, in one embodiment, for example blood sample is carried out the antibody calibrating, its step is shown in Figure 35 A, 35B, 35C and 35D.This antibody calibrating installation 600 comprises corpse or other object for laboratory examination and chemical testing circulation raceway groove 610, labelled antibody groove 620, sample cell 630, abacus 640, very first time retardation valve 622 and the second time-lag valve 632.And corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 comprises that step zone 612, antibody array zone 614 open valve 616 with breakaway-element.This step zone 612 comprises the zone 611,613 and 615 of various flows road caliber.And above-mentioned zone 611 has bigger runner caliber, and the zone 61 3 then has less runner caliber, for example less than the runner caliber of above-mentioned zone 611.
At first, shown in Figure 35 A, blood sample 635 is written in the sample cell 630, then, blood sample 635 can flow to zone 613 at a slow speed via the second time-lag valve 632, and this second time-lag valve 632 will cut out and no longer allow blood sample 635 flow in the corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 subsequently.And, therefore can obtain deciding the blood sample 635 of volume because the zone 613 inner flow passage calibers of corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 are fixed.
Then, shown in Figure 35 B, labelled antibody 625 is written in the labelled antibody groove 620, then, labelled antibody 625 can flow to zone 611 at a slow speed via very first time retardation valve 622, and this very first time retardation valve 622 will cut out and no longer allow labelled antibody 625 flow in the corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 subsequently.And, therefore can obtain deciding the labelled antibody 625 of volume because the zone 611 inner flow passage calibers of corpse or other object for laboratory examination and chemical testing circulation raceway groove 610 are fixed.
Then, shown in Figure 35 C, washing fluid 645 is added in the abacus 640, subsequently, shown in Figure 35 D, after breakaway-element opened valve 616 and interrupt, produce absorption affinity, with a corpse or other object for laboratory examination and chemical testing circulate washing fluid 645 in the raceway groove 610, be positioned at the labelled antibody 625 and blood sample 635 absorption that are positioned at zone 613 and process antibody array zone 614 in zone 611, can reach the effect that antibody is examined and determine.
In the above-mentioned antibody calibration method, a corpse or other object for laboratory examination and chemical testing circulates the interior runner caliber convergent of raceway groove and increases the downstream capillary force, and with step (step) design, cooperates the time-lag valve, when liquid bath flows into the particular conduit interval, reaches liquid and decides the volume function.Injecting sample in sample cell in regular turn, labelled antibody, is opened breakaway-element and is opened valve behind abacus in labelled antibody groove and washing fluid, and liquid is because of the driving of capillary force, the antibody array district that flows through in regular turn carries out in conjunction with (binding), mark and flushing supervisor.
The design of above-mentioned time-lag valve also can be used in for example Figure 36 A and 36B illustrate and be undertaken in the device that blood cell separates by centrifugal force.In the separation of whole blood device 660 of Figure 36 A and 36B retardation setup time valve, comprise separation of whole blood deferent 662.On separation of whole blood deferent 662 internal side wall setup time retardation valve 664.This separation of whole blood deferent 662 then is according to rotating shaft 666 rotations, produces centrifugal force (Centrifugal Force) in centrifugal force direction (Centrifugal Direction) 668.In separation of whole blood deferent 662, then inject whole blood sample (Whole Blood Sample) 661, when high speed centrifugation, the blood cell of whole blood sample 661 can produce blood cell district (Blood Cell Area) 663 and blood plasma district (Blood PlasmaArea) 665 toward centrifugal regional centralized.In the high speed centrifugation process, time-lag valve 664 outer water-soluble retardation layers can dissolve, and treat after the separation super can expand after absorbing resin (SAP) absorption portion water, and can stop blood cell district 663 to be mixed with diffusion between the blood plasma district 665.
Although above discussed some example, other enforcements and application also belong to the category of following claim.For example, in the vacuum pump 90 of Figure 1A and Figure 1B, container 100 can contain lower pressure region and be not to contain vacuum area.As long as container 100 gas inside pressure are lower than the gaseous tension of container 100 outsides, when container 100 disconnects, the pressure in the zone 106 of container 100 outsides will descend, thereby produce along the attractive force of a direction towards container 100 draw fluid.Above-described kapillary can be substituted by the kapillary made from other hard brittle materials, such as fragility plastic cement, quartz and ceramic.
Claims (68)
1. method comprises:
Control fluid flowing in raceway groove, comprise and disconnect first container in described raceway groove, to produce pressure differential, make described fluid in described raceway groove, move, described first container is made by hard brittle material, wherein said first container (a) defines the space in described first container, described space has the gaseous tension of the gaseous tension that is different from described first external container, or (b) comprise first material, described first material is separated with second material before described first container disconnects, described first material and described second material through selecting so that after described first material and the interaction of described second material, produce gas immediately.
2. the method for claim 1, the described space in wherein said first container has the gaseous tension of the described gaseous tension that is higher than described first external container.
3. method as claimed in claim 2, wherein control described fluid described flow to be included in promote described fluid in the described raceway groove and make it away from described first container that is disconnected.
4. the method for claim 1, the described space in wherein said first container has the gaseous tension of the described gaseous tension that is lower than described first external container.
5. method as claimed in claim 4, the described described fluid that comprises in the described raceway groove of attraction that flows of wherein controlling described fluid makes it towards described first container that is disconnected.
6. method as claimed in claim 5, wherein said fluid comprises blood, and control described blood described flow comprise make described blood through filtrator with the occlude blood cell and allow blood plasma through described filtrator and enter described raceway groove.
7. method as claimed in claim 5 also comprises and carry out the colorimetric calibrating in described raceway groove when described fluid flows.
8. method as claimed in claim 5, also comprise second container, described second container (a) defines the space in described second container, described space has the gaseous tension of the gaseous tension that is higher than described second external container, or (b) comprise the 3rd material, described the 3rd material is separated with the 4th material before described second container disconnects, described the 3rd material and described the 4th material through selecting so that produce gas immediately after described the 3rd material and the interaction of described the 4th material.
9. method as claimed in claim 8, the described described fluid that comprises in the described raceway groove of promotion that flows of wherein controlling described fluid makes it away from described second container.
10. method as claimed in claim 5, described flow of wherein controlling described fluid comprises the fluid adsorption material that expands immediately by volume after the part of using the described fluid of absorption and stops additional fluid to move along a certain direction to pass through described raceway groove.
11. a fluid means comprises:
Raceway groove; And
First container, described first container produces pressure differential in described raceway groove when described first container disconnects, described first container is made by hard brittle material, wherein said first container (a) defines the space in described first container, described space has the gaseous tension of the gaseous tension that is different from described first external container, or (b) comprise first material, described first material is separated with second material before described first container disconnects, described first material and described second material through selecting so that produce gas immediately after described first material and the interaction of described second material.
12. fluid means as claimed in claim 11, also comprise second container, described second container (a) defines the space in described second container, described space has the gaseous tension of the gaseous tension that is different from described second external container, or (b) comprise the 3rd material, described the 3rd material is separated with the 4th material before described second container disconnects, described the 3rd material and described the 4th material through selecting so that produce gas immediately after described the 3rd material and the interaction of described the 4th material.
13. fluid means as claimed in claim 12, described space in wherein said first container has the pressure of the described pressure that is higher than described first external container, and the described space in described second container has the pressure of the described pressure that is lower than described second external container.
14. fluid means as claimed in claim 12, described space in wherein said first container has the pressure of the described pressure that is lower than the described first closed container outside, and described second container comprises first material, described first material is separated with second material before described second container disconnects, described first material and described second material through selecting so that produce gas immediately after described first material and the interaction of described second material.
15. fluid means as claimed in claim 14 also comprises filter membrane with occlude blood cell and permission blood plasma process.
16. fluid means as claimed in claim 11, wherein said hard brittle material comprise at least a in the following material: quartz, glass, pottery, plastic cement, and both or both the above compound in quartz, glass, pottery and the plastic cement.
17. fluid means as claimed in claim 11, wherein said raceway groove are to be defined by the wall made from the pliability material.
18. produce the material of gas when fluid means as claimed in claim 11, wherein said first container comprise heating.
19. fluid means as claimed in claim 11, wherein said first container comprise when heating from the solid-state material that sublimes up into gaseous state.
20. a fluid means comprises:
Define first material of first area;
Define second material of second area, described second area and described first area are separated; Connector, be coupled between described first area and the described second area, described connector comprises hard brittle material and has the open end and closing end, described open end is placed in the described second area, described closing end is placed in the described first area, described first area cuts off by the described closing end and the described second area of described connector, so that when the described closing end of described connector disconnects, described connector defines from described first area to the path of described second area described connector through configuration.
21. a fluid means comprises:
The raceway groove that comprises dilation, described dilation has the diameter greater than the adjacent part of described raceway groove; And
Be placed in the material in the described dilation, described material has the volume of not blocking the path of described fluid before fluid-absorbent, wherein said material adsorb that volume after the part of described fluid expands immediately so that the described material blocking-up in the back of expanding additional fluid through the path of described raceway groove.
22. fluid means as claimed in claim 21, wherein said material comprises super absorbent polymer.
23. a fluid means comprises:
First reservoir that contains first fluid;
Second reservoir that contains second fluid;
The tap drain road;
First branch's raceway groove, described first branch's raceway groove couple described first reservoir to described tap drain road;
Second branch's raceway groove, described second branch's raceway groove couple described second reservoir to described tap drain road;
First single uses pump, and described first single uses pump to produce pressure differential to move the one or both in described first fluid and described second fluid when the container in described first single use pump disconnects; And
Second single uses pump, and described second single uses pump to produce pressure differential to move the one or both in described first fluid and described second fluid when the container in described second single use pump disconnects.
24. fluid means as claimed in claim 23, wherein said first container (a) defines the space in described first container, described space has the gaseous tension of the gaseous tension that is different from described first external container, or (b) comprise first material, described first material is separated with second material before described first container disconnects, described first material and described second material through selecting so that produce gas immediately after described first material and the interaction of described second material.
25. fluid means as claimed in claim 23, also comprise self-closing valve, described self-closing valve comprises initially having small size so that described first fluid can be through the material of described valve, and described material increases volume and further passes through the path of described valve to stop described fluid after the part of the described first fluid of absorption.
26. fluid means as claimed in claim 23, also comprise valve, described valve has the connector made from hard brittle material, wherein when described connector is intact, described valve stops described first fluid to enter described tap drain road, and when described connector disconnects, produce path and enter described tap drain road to allow described first fluid.
27. fluid means as claimed in claim 23 also comprises: the 3rd reservoir, described the 3rd reservoir contains three-fluid; And the 3rd branch's raceway groove, described the 3rd branch's raceway groove couples described the 3rd reservoir to described tap drain road.
28. fluid means as claimed in claim 23 also comprises sensing area, described sensing area is coupled to described tap drain road, and described sensing area comprises whether the decidable certain material is present in the sensing agent in the described first fluid.
29. a method comprises:
Provide a plurality of suction pipes so that the fluid of the scheduled volume of can taking a sample, each suction pipe comprises:
Raceway groove, and
Container, described container produces pressure differential when disconnecting in described raceway groove, described container is made by hard brittle material, described container defines the space in the described container, described space has the gaseous tension less than the gaseous tension of described external container, its break described container can in described raceway groove, produce scheduled volume pressure differential so that the fluid of scheduled volume drawn to described raceway groove.
30. a method comprises:
Make fluid to flow to second area from the first area, described first area is coupled to described second area by the connector with open end and closing end, described open end is placed in the described second area, described closing end is placed in the described first area, the described closing end of described first area by described connector cuts off with described second area, wherein make described fluid to flow to comprise the described closing end that disconnects described connector with form the described connector of process from described first area the path of described second area extremely;
By using the part that the material of volumetric expansion adsorbs the described fluid of the described connector of flowing through behind the described fluid of absorption; And
Use and describedly block the additional fluid described connector of further flowing through through the material that expands.
31. a method comprises:
Fluid is flowed in raceway groove, described raceway groove comprises the material that volume expands immediately after the part of adsorbing described fluid, described fluid is flowed comprise flow through described material and use described material to adsorb the second portion of described fluid of the first that makes described fluid in described raceway groove, thereby make described material volume expand; And
By using the described path of blocking the described raceway groove of additional fluid process through the material that expands.
32. method as claimed in claim 31, the closing end that also comprises disconnect connector, describedly to make additional fluid in described raceway groove, to flow through the material that the expands described connector of flowing through by described fluid is walked around, wherein before disconnecting described closing end, described connector has the open end in the first that is placed in described raceway groove and is placed in closing end in the second portion of described raceway groove, and described first and described second portion are separated by described material through expanding.
33. method as claimed in claim 32, comprise also that the material of volumetric expansion adsorbs the part of the described fluid of the described connector of flowing through behind the described fluid of absorption by using, and use and describedly block the additional fluid described connector of further flowing through through the material that expands.
34. method as claimed in claim 31, wherein said material comprises super absorbent polymer.
35. a method comprises:
Make fluid through raceway groove, described raceway groove comprises first self-closing valve and second self-closing valve, described first self-closing valve and described second self-closing valve are spaced apart from each other, and each self-closing valve comprises the fluid adsorption material that volume expands immediately after the part of adsorbing described fluid;
The part of adsorbing described fluid by the described fluid adsorption material that uses in described first self-closing valve and described second self-closing valve; And
The described volume of described fluid adsorption material of expanding further passes through the path of described raceway groove with the blocking-up additional fluid, the fluid of maintenance scheduled volume in described first self-closing valve of described raceway groove and the part between described second self-closing valve.
36. method as claimed in claim 35 also comprises and uses capillary force to draw described fluid to make it through described raceway groove.
37. a method comprises:
First container that blocking-up is made by hard brittle material is to produce pressure differential in raceway groove, make first fluid move to first section of described raceway groove from first reservoir, described first container (a) defines the space in described first container, described space has the gaseous tension of the gaseous tension that is different from described first external container, or (b) comprise first material, described first material is separated with second material before described first container disconnects, described first material and described second material through selecting so that produce gas immediately after described first material and the interaction of described second material; And
Second container that blocking-up is made by hard brittle material to be to produce pressure differential in raceway groove, make the moving and through second section of described raceway groove to small part of described first fluid.
38. method as claimed in claim 37 also comprises first valve that blocking-up made by hard brittle material to produce first path, described first path connects second reservoir to described raceway groove, and described second reservoir contains second fluid.
39. method as claimed in claim 38 wherein makes described second fluid move to described second section of described raceway groove from described second reservoir by blocking the described pressure differential that described second container produces.
40. method as claimed in claim 38 also comprises second container that blocking-up made by hard brittle material to produce pressure differential, makes described second fluid move to described second section of described raceway groove from described second reservoir.
41. method as claimed in claim 40 also comprises second valve that blocking-up made by hard brittle material to produce alternate path, described alternate path connects the 3rd reservoir to described raceway groove, and described the 3rd reservoir contains three-fluid.
42. method as claimed in claim 41 also comprises the 3rd container that blocking-up made by hard brittle material to produce pressure differential, makes described three-fluid move to described second section of described raceway groove from described the 3rd reservoir.
43. method as claimed in claim 37, described second section of wherein said raceway groove comprise in order to judge whether specific judgement material is present in the sensing agent in the described first fluid.
44. method as claimed in claim 37, wherein said first container define the space in described first container, described space has the gaseous tension of the gaseous tension that is lower than described first external container.
45. method as claimed in claim 44, wherein said second container define the space in described second container, described space has the gaseous tension of the gaseous tension that is lower than described second external container.
46. method as claimed in claim 44, wherein said second container defines the space in described second container, described space has gaseous tension, described gaseous tension (a) is higher than the gaseous tension of the gaseous tension of described second external container, or (b) comprise first material, described first material is separated with second material before described second container disconnects, described first material and described second material through selecting so that produce gas immediately after described first material and the interaction of described second material.
47. a method comprises:
Operating first single simultaneously uses the pump and second single to use pump with first's to the first raceway groove of drawing sample fluid and second portion to the second raceway groove of drawing described sample fluid, comprise that first container of blocking in described first single use pump is to produce pressure differential, make the described first of described sample fluid move to described first raceway groove from reservoir, and block described second single and use second container in the pump, make the described second portion of described sample fluid move to described second raceway groove from described reservoir to produce pressure differential; And
Operating the 3rd single simultaneously uses pump and the 4th single to use pump to draw first buffer solution to described first raceway groove and draw second buffer solution to described second raceway groove.
48. method as claimed in claim 47 also comprises and operates the 5th single simultaneously and use pump and the 6th single to use pump to draw tri buffer solution to described first raceway groove and draw the 4th buffer solution to described second raceway groove.
49. method as claimed in claim 47, also comprise and use pump to operate the 5th single simultaneously with described first single to use pump drawing third part to the triple channel of described sample fluid, and use pump to operate the 6th single simultaneously with described the 3rd single and use pump to draw tri buffer solution to described triple channel.
50. a fluid means comprises:
The raceway groove that comprises dilation, described dilation has the diameter greater than the adjacent part of described raceway groove;
Be placed in the material in the described dilation; And
The retardation material places between the path of the material of described dilation and described fluid,
Wherein after described retardation material dissolves, place the material of described dilation to have the volume of before fluid-absorbent, not blocking the path of described fluid, wherein said material adsorb that volume after the part of described fluid expands immediately so that the described material blocking-up in the back of expanding additional fluid through the path of described raceway groove.
51. fluid means as claimed in claim 50, wherein said material comprises super absorbent polymer.
52. fluid means as claimed in claim 50, wherein said retardation material is a water-soluble material.
53. fluid means as claimed in claim 50, wherein said retardation material are sucrose piece or sucrose sheet.
54. a fluid control method comprises:
Described fluid is flowed in raceway groove, described raceway groove comprise the material that volume expands immediately after the part of adsorbing described fluid and place described material and described fluid passage between the retardation material,
After the described retardation material dissolves, place the material of described dilation to have the volume of before fluid-absorbent, not blocking the path of described fluid, wherein said material adsorb that volume after the part of described fluid expands immediately so that the described material blocking-up in the back of expanding additional fluid through the path of described raceway groove.
55. fluid control method as claimed in claim 54, wherein said material comprises super absorbent polymer.
56. fluid control method as claimed in claim 54, wherein said retardation material is a water-soluble material.
57. fluid control method as claimed in claim 54, wherein said retardation material are sucrose piece or sucrose sheet.
58. fluid control method as claimed in claim 54 wherein places the retardation material between described material and the described fluid passage to be to use the immersion type coating method to form.
59. fluid control method as claimed in claim 54 wherein places the retardation material between described material and the described fluid passage to be to use the drum-type coating method to form.
60. fluid control method as claimed in claim 54 wherein places the retardation material between described material and the described fluid passage to be to use rotary coating method to form.
61. fluid control method as claimed in claim 54 wherein places local coating of retardation material or comprehensive the coating wherein between described material and the described fluid passage.
62. an antibody calibrating installation comprises:
Corpse or other object for laboratory examination and chemical testing circulation raceway groove;
The labelled antibody groove is connected to described corpse or other object for laboratory examination and chemical testing circulation raceway groove through very first time retardation valve;
Sample cell is connected to described corpse or other object for laboratory examination and chemical testing circulation raceway groove through the second time-lag valve; And
Abacus, be connected to described corpse or other object for laboratory examination and chemical testing circulation raceway groove, comprise the step zone in the wherein said corpse or other object for laboratory examination and chemical testing circulation raceway groove, valve is opened with breakaway-element in the antibody array zone, the runner caliber in wherein said step zone is the design of convergent current road caliber, wherein said first and second time-lag valve comprises water uptake expansion material and water-soluble retardation layer, and the water-soluble retardation layer in the described time-lag valve is via after dissolving, water uptake expansion material in the described time-lag valve is understood imbibition and is stoped described labelled antibody groove and sample cell, and the diffusion of circulating between the raceway groove with a described corpse or other object for laboratory examination and chemical testing mixes.
63. an antibody calibration method is applicable to the antibody calibrating installation, described antibody calibrating installation comprises corpse or other object for laboratory examination and chemical testing circulation raceway groove, comprises that the runner caliber is a step zone of convergent current road caliber design in the wherein said corpse or other object for laboratory examination and chemical testing circulation raceway groove, and described method comprises:
Sample is flow to secondary fluid course caliber zone in the described convergent current road caliber via the second time-lag valve, and the described subsequently second time-lag valve cuts out;
Labelled antibody is flow to big runner caliber zone in the described convergent current road caliber via very first time retardation valve, wherein the runner caliber in big runner caliber zone is greater than the runner caliber in this secondary fluid course caliber zone, the retardation valve cuts out the described very first time subsequently, wherein said first and second time-lag valve comprises water uptake expansion material and water-soluble retardation layer, and the water-soluble retardation layer in the described time-lag valve via dissolving after, the water uptake expansion material in the described time-lag valve can imbibition and stop described labelled antibody and sample to be diffused into a described corpse or other object for laboratory examination and chemical testing to circulate in the raceway groove;
Washing fluid is connected in the described corpse or other object for laboratory examination and chemical testing circulation raceway groove; And
Utilize absorption affinity, described washing fluid is adsorbed onto in the described corpse or other object for laboratory examination and chemical testing circulation raceway groove, and via described secondary fluid course caliber zone and big runner caliber zone, with the antibody array zone of flowing through of the labelled antibody in blood sample in the described secondary fluid course caliber zone and the described big runner caliber zone, to finish described antibody calibrating.
64. as the described antibody calibration method of claim 63, wherein said water-soluble retardation layer material is a water-soluble material.
65. as the described antibody calibration method of claim 63, wherein said water-soluble retardation layer material is sucrose piece or sucrose sheet.
66. a separation of whole blood device comprises;
The separation of whole blood deferent, and on described separation of whole blood deferent internal side wall setup time the retardation valve, wherein said time-lag valve comprises water uptake expansion material and water-soluble retardation layer; And
Rotating shaft, described separation of whole blood deferent rotates according to described rotating shaft, produce centrifugal force in the centrifugal force direction, will be after via high speed centrifugation at the whole blood sample in the described separation of whole blood deferent, can produce blood cell district and blood plasma district, and the water-soluble retardation layer in the described time-lag valve via dissolving after, the water uptake expansion material in the described time-lag valve can imbibition and stop described blood cell district to be mixed with diffusion between the described blood plasma district.
67. as the described separation of whole blood device of claim 66, wherein said water-soluble retardation layer material is a water-soluble material.
68. as the described separation of whole blood device of claim 66, wherein said water-soluble retardation layer material is sucrose piece or sucrose sheet.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83128506P | 2006-07-17 | 2006-07-17 | |
| US60/831,285 | 2006-07-17 |
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| CN101109759A true CN101109759A (en) | 2008-01-23 |
| CN101109759B CN101109759B (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2006101495740A Expired - Fee Related CN101109761B (en) | 2006-07-17 | 2006-11-21 | Fluidic device and control method thereof |
| CN200710136828XA Expired - Fee Related CN101109759B (en) | 2006-07-17 | 2007-07-17 | Fluidic device and control method thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101495740A Expired - Fee Related CN101109761B (en) | 2006-07-17 | 2006-11-21 | Fluidic device and control method thereof |
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| Country | Link |
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| CN (2) | CN101109761B (en) |
| TW (4) | TWI336781B (en) |
Cited By (2)
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|---|---|---|---|---|
| CN103398995A (en) * | 2012-09-07 | 2013-11-20 | 欧普图斯(苏州)光学纳米科技有限公司 | Integrated equipment capable of realizing chemical separation and light dispersion |
| CN103529195A (en) * | 2013-10-24 | 2014-01-22 | 山东大学 | Detection method applied to measurement of trace target materials |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11278225B2 (en) * | 2015-08-12 | 2022-03-22 | University Of Tasmania | Liquid collection device |
| JP6529667B2 (en) * | 2016-01-22 | 2019-06-12 | ヒューレット−パッカード デベロップメント カンパニー エル．ピー．Ｈｅｗｌｅｔｔ‐Ｐａｃｋａｒｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｃｏｍｐａｎｙ， Ｌ．Ｐ． | Fluid detection with control of particle aggregation in the detection area |
| CN111110951A (en) * | 2020-01-09 | 2020-05-08 | 中国人民解放军总医院 | Micro-control orbital vein quantitative blood taking, injection and analysis device |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100865105B1 (en) * | 1999-06-28 | 2008-10-24 | 캘리포니아 인스티튜트 오브 테크놀로지 | Microfabricated elastomeric valve and pump systems |
| US6527003B1 (en) * | 2000-11-22 | 2003-03-04 | Industrial Technology Research | Micro valve actuator |
| US6521188B1 (en) * | 2000-11-22 | 2003-02-18 | Industrial Technology Research Institute | Microfluidic actuator |
| US6453928B1 (en) * | 2001-01-08 | 2002-09-24 | Nanolab Ltd. | Apparatus, and method for propelling fluids |
| US20060044084A1 (en) * | 2002-03-23 | 2006-03-02 | Joseph Cefai | Macromechanical components |
| JP2004069397A (en) * | 2002-08-02 | 2004-03-04 | Nec Corp | Analysis chip and analytical apparatus |
| US20060245978A1 (en) * | 2003-04-04 | 2006-11-02 | Koninklijke Philips Electronics N.V. | Fluid partitioning in multiple microchannels |
| WO2005036182A1 (en) * | 2003-10-15 | 2005-04-21 | Matsushita Electric Industrial Co., Ltd. | Method of passing fluid in capillary chip |
| EP1824600B1 (en) * | 2004-10-18 | 2016-12-28 | Life Technologies Corporation | Fluid processing device including size-changing barrier |
-
2006
- 2006-11-21 TW TW95142968A patent/TWI336781B/en not_active IP Right Cessation
- 2006-11-21 CN CN2006101495740A patent/CN101109761B/en not_active Expired - Fee Related
- 2006-11-21 TW TW98128572A patent/TW201000901A/en unknown
- 2006-11-21 TW TW98128573A patent/TWI404929B/en not_active IP Right Cessation
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2007
- 2007-07-10 TW TW96125027A patent/TWI338081B/en not_active IP Right Cessation
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103398995A (en) * | 2012-09-07 | 2013-11-20 | 欧普图斯(苏州)光学纳米科技有限公司 | Integrated equipment capable of realizing chemical separation and light dispersion |
| CN103398995B (en) * | 2012-09-07 | 2015-12-23 | 欧普图斯(苏州)光学纳米科技有限公司 | The integrated equipment of Chemical Decomposition and light scattering can be realized |
| CN103529195A (en) * | 2013-10-24 | 2014-01-22 | 山东大学 | Detection method applied to measurement of trace target materials |
| CN103529195B (en) * | 2013-10-24 | 2014-08-06 | 山东大学 | Detection method applied to measurement of trace target materials |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201000901A (en) | 2010-01-01 |
| TW200813324A (en) | 2008-03-16 |
| TWI404929B (en) | 2013-08-11 |
| CN101109761B (en) | 2012-05-30 |
| TW200806988A (en) | 2008-02-01 |
| CN101109761A (en) | 2008-01-23 |
| TWI336781B (en) | 2011-02-01 |
| CN101109759B (en) | 2012-05-30 |
| TWI338081B (en) | 2011-03-01 |
| TW200951436A (en) | 2009-12-16 |
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