TWI301888B - - Google Patents

Description

Case number 94110748

1301888 V. INSTRUCTION DESCRIPTION (1) [Technical Field] The present invention relates to a biochemical test strip for personal use, especially a blood biochemical test strip which can be used multiple times, and can be used as a drop of jfe * Simultaneous measurement of biochemical detection of two or more identical or different test items. [Prior Art] At present, all the blood glucose test strips on the market are _ ^ one foot, and the test piece that is used up and lost. Such test strips are too expensive and unaffordable for patients with diabetes in developing or untapped countries, and cannot be routinely tested for blood sugar according to their condition, even life-threatening. They really need a cheaper blood glucose test strip. At the same time, the accuracy of blood glucose test strips currently on the market is not enough, limited to the materials and methods used by them, often resulting in more than 30% error. Moreover, in addition to a few brands in Europe and America, it takes 15 to 30 seconds to measure. Accordingly, the main object of the present invention is to provide a cheaper, more accurate, and superior test piece. Blood glucose test with a shorter detection time and a shorter test time. Another main object of the invention is the use of a blood glucose test or a different test sample. Blood:: The purpose of measuring several identical values on the drop is A & $ I Μ Μ 测 测 测 测 测 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 相同 疋 疋 疋 疋 疋 疋 疋 疋 疋 疋 疋 疋 疋More than a few blood collection:: The average value is increased: The blood can be measured. Different kinds of inspection products read wood ^ pain · 'use - drop the same project test, but do not have to continue, the purpose 疋 in order to be able to do at the same time So far, the world has produced right blood to reduce the pain of blood collection. ‘Any other patent is presented in a single

1301888

Emperor No. 94110748 V. Invention Description (2) Law == 匕 Energy: The structure of the sub-use is requested (US invention patent n\burden. Our previous patent application ^ ^ Λ / 7 / / Λ 1 0 / 704 ^ 7 〇1^^ τ case is different. Electric billion.. film, but the content and this application

Shiro Nankai et al., Shen Guzhen’s cover net, a kind of national patent 5, 1 20, 420 in a Η 1 · ·· Shi Xi reveals that there are eve electrode wires on the ° type film, so as to simultaneously detect several The blood glucose concentration 'takes the average value again to increase = number of coincidences:: The method requires a relatively large amount of blood, and it is not feasible. At the same time, this rare party, = heart is self-testing and measuring costs. The block method also does not help to reduce the user's inspection. θ: There are a lot of research reports and patents to propose various methods to improve the accuracy or speed of detection*. For example, Thomas γ· s. shen “,] = Patent 6, 491, 803 mentions the use of nano metal powder to increase conductivity and reduce the reaction time, but still takes fifteen seconds (Biosensors &; Βΐ〇β1βοΪΓΟηί€δ, 1 3 : 333-339Λ 996^ ί Ϊ : Extreme surface force: Into the platinum powder can increase the conductivity to improve the measurement' again, but these effects are not good enough. The beauty of CD ffisiey et al _ Patent No. 6,541,216 states that the use of plasma to treat the surface and surface of the silver and carbon electrodes can increase the accuracy of the measurement of the test piece. However, these methods do not achieve the cheaper and more accurate requirements of the patent. And the target of measuring faster speed is not the method adopted in this patent. [Invention] For the above purpose, the present invention provides a life that can be used multiple times.

Page 6 1301888 Case No. 94110748 V 'Invention Description (3) Amendment

"Chemical, test film, which mainly contains a one-time, use-and-drop type of each piece that can be used multiple times: the structure of the physical inspection office - the special test piece and the chemical carrier contained in it" The sensing device is used, and the component of the test product is detected and the productivity is determined according to the electrical effect generated by the test product in the reaction. The structure of the present invention is formed by sticking four layers of sheets, and the sheet: various The space formed by the punching can produce three identical reaction zones, and each reaction zone is provided with a biochemical reaction carrier, which requires only 2 // 1 of the blood volume, '= . By the principle of siphon, the blood will be inhaled into the reaction zone. Immediately, an electrochemical reaction is carried out with the borrowing reaction carrier thereon, and the blood glucose concentration of the blood test product can be known by analyzing the electrochemical reaction. The space formed by the various other pores of the sheet can also The test piece can be used in the middle of the sheet due to the stress concentration factor, and is broken, so as to benefit the second and third times, and then use the other unused g. The uppermost sheet has two positive and negative conductive electrodes for conduction. Electrochemical signal, and the electrode lead is It is calculated that the positive and negative electrodes of each electrochemical zone 2 have the same electrical power as the positive and negative electrodes on the measuring electrode head. The resistance of the same resistance is the key factor that can be used repeatedly for the test piece disclosed in the present invention, and is also indispensable. The element, this method is adopted by the world. The positive and negative electrodes of each electrochemical reaction zone will transmit the electrochemical reaction signal generated by the chemical reaction in the blood reaction to the positive and negative electrodes on the measuring electrode head. The blood glucose tester interprets the blood glucose reading of the blood test. As for the hydroxypropyl methyl cellulose, the chemical reaction substance is evenly distributed, which is in accordance with U.S. Patent 5,12,42

The formula for the chemical reaction material we have developed exclusively contains oxyhydrin 1301888. Case No. 94110748. 5. Invention Description (4) National Patent No. 374 1 16 and U.S. Patent 6, 41 3, 394B1 (Microcrystalline cellulose) is different, this is also the first time we have adopted the world. In addition to this, we also add multi-wall and single-wall (Multi-wa丨led and single wal led) carbon nanotubes to increase the conductivity rate and reduce the detection time. It can adjust the reaction time from the general three. Ten seconds to four seconds or even one second 'and can provide the appropriate surface tension and surface energy to prevent blood I solid, this method is the first time we use the world. In this way, the test piece disclosed by the present invention can be reused many times, and can quickly and accurately. In addition, if the test piece formed by the above four layers is attached to the same four layers or eight layers on the uppermost layer, but the siphon blood inlet positions are all in the same position, a drop of jk can be siphoned to two. Or three different, independent anti-wide areas 'do the same or different biochemical reaction tests, so use a drop of blood $ to simultaneously measure two or more identical or different samples. If you measure 3 with two drops of the same test at the same time, you can take the average value to increase its accuracy; but if you measure two or more different check points simultaneously with one drop of blood, you can reduce each time. The pain of blood collection. Accordingly, the blood glucose test strip of the present invention can reduce the cost of testing each time the user uses, and also has the highest accuracy and the shortest test time.曰 [Embodiment] In order to enable the reviewing committee to have a deeper understanding and understanding of the structure and characteristics of the present invention, the feasible embodiments and the drawings are detailed.

1301888 Main Moonlight Correction_Case No. 94110748 V. Invention Description (5) As follows: Refer to the first, second and third figures, the biodetection test piece 1 which can be used multiple times according to the present invention 〇, is made of four layers of π, 12, 1 3, 1 4, and placed between the 丨丨 and 丨 2 with two wires 1 5, 1 6 ' wire 15 connected to three reference electrodes丨51, 丨5 2, 丨5 3, and the wire 16 is connected to the three working electrodes 1 6 1 , 1 6 2, 1 6 3 (as shown in the second figure). The sheet 11 has two punching holes 17 and 18, which can generate stress concentration to break the test piece. The sheet 11 has a concave portion 9 to indicate the blood level. The sheet is divided into three pieces. The small sheets 1 21, 1 2 2, and 1 2 3 are attached to the wires 15 and 16 and the sheet 11; the gap between the three small sheets 121, 122, and 123 is for the combined test piece. 3) C), the three small sheets 121, 122, 123 each have three punching holes 201, 202, 203 for placing chemical carriers 251, 252, 253 on the working electrodes 161, 162, 163 and the reference electrode 1 5 1 , 1 5 2, 1 5 3 (as in the third figure A) to become three different reaction zones, and a recess in each of the three small sheets 1 2 1 , 1 22, 1 2 3 191, 192, 193 to indicate the location of the blood. _ The sheet 13 is divided into three small sheets 131, 1 32, 133 and attached to the sheet 12. Similarly, there is a gap between the three small sheets 131, 1 32, 133 for the combined test piece to be broken; Having three recesses 3 0 1 , 30 2 , 3 0 3 thereon allows the blood 40 to electrochemically react with the chemical carriers 251, 252, 253 by siphoning (eg, Figures A and B). Therefore, the test piece only needs 2 // 1 jk liquid volume per test, by siphoning the original

Page 9 1301888 _____ Case No. 94110748_Year η Amendment 5, Invention Description (6) The blood will be inhaled into the reaction zone and then electrochemically react with the biochemical carrier thereon, and by analyzing the electrochemical reaction As a result, the blood glucose concentration of the blood test product can be known. The sheet 14 is divided into three small sheets 1 41 , 1 4 2 , 1 4 3 and attached to the sheet 13 , and has three concave portions 194, 195, 196 thereon for marking the blood feeding position; 4 0 1 , 4 0 2, 4 0 3 as the siphon principle blood outlet. The conductive electrodes 15, 16 are designed to have the same wire length, that is, the same resistance, as the reference electrodes 15i, 152, and 153 to the electrode tip 51, and the working electrodes 1 6 1 , 1 6 2 6 3 to the electrode tip 5 2 need to have the same wire length 'that is the same resistance, so the biochemical reaction results in these three different reaction zones will be consistent. As described above, the fact that each of the conductive electrodes 15 and 16 has the same resistance is a key factor for the multiple use of the test piece disclosed in the present invention, and is an indispensable element. This method is first adopted worldwide. In the above structure, the sheets 11, 12, 13 and 14 may be plastic, ceramic substrate, cardboard or FRP (glass fiber reinforced plastic) material; and the conductive electrodes 15, 16 may be screen printing processes. Silver-glued or carbon-adhesive wires can also be used in the additive process to generate the required conductive circuits by chemical reaction; or by subtractive process, chemically reacting the surface locally. Useless conductors are removed; then precious metals such as gold, platinum, and other precious metals are replaced by chemical substitution reactions on the substrate by chemical immersion plating (commonly known as gold), and more than one hundred pieces are produced at one time by a sufficient method. In the case of mass production, the thickness of the precious metal is averaged, that is, the resistance of the three reaction zones on each test piece can be

1301888 Case j Tiger 94110748 V. Invention Description (7) Maintaining within one percent of the ##. The method of our decision is different from the US patent 5,437,999. It is also different from Huamin Guozhi 4Ϊ1 and Kamin Xuli 496110, because the latter is plated with precious metals by electroplating, and the right 4 u μ + soil ^ ^ All the cymbals need to be connected to the same electrode (not required by our method), combined with 4 μL ^ main a β - one 曰 I into 5 pieces of precious metal plating thickness uneven, and the resistance in the three reaction areas Both exceed 30% of the error, so the error is too large to be applicable. As used in the above construction, the use of the present invention in the blood 40 into the test strip 10 is not shown in the drawings shown in Figs. When blood 4〇 is siphoned by the concave 3〇1 at the right end of the test piece 10 and filled with the reaction zone of the punch 2〇1 repair (third picture A to B), after a few seconds of blood reading After the value appears, the test piece of the used first reaction zone can be broken (as shown in the third figure C), and the unused second reaction zone (such as the third figure D) is exposed. One blood test. By analogy, after the second reaction zone is used up, the test piece of the second reaction zone can be broken to expose the third unreacted reaction zone, and then the test piece can be further processed. Three times of blood testing, the purpose of the same test piece can be used multiple times. Referring to FIG. 4 and FIG. 5 again, there are shown three embodiments in which the three sets of the same 10 and different lengths are overlapped and assembled into one piece as shown in the first figure, which allows blood to enter. The punching holes 〇1 have the same inlet position, and the punching holes 404, 40 5, and 40 6 as the air outlets are at least from the top to the bottom layer, so that one or two different identical items can be simultaneously made with one drop of blood. Detection. When the embodiment is used, as shown in the fifth figure, when a drop of blood 40 is inhaled and filled with three reaction zones &

1301888 - - 94j, 10748_ 月月曰__ Amendment_ V. Inventive Note (8) c ', the chemical carrier 251 in each reaction zone will simultaneously react electrochemically with the blood inhaled in each reaction zone. Wherein the chemical carriers 251 in the three reaction zones a, 匕, c may be the same type to produce the same reading value, and then averaged to obtain better accuracy; or different types, Three different readings were obtained with one drop of blood. Similarly, the same test piece can be broken and reused after the first test is used up. The test piece structure described above may also be modified as shown in the sixth and seventh figures. As shown in the sixth embodiment, the test piece 1 is designed to be adhered by three layers of sheets 11, 12, and 13 In which the concave blood inlets 71, 72, . . . are disposed on the second layer of the sheet 12, and are designed on the other side of the extension direction electrode heads 5 i, 5 2 to cause blood to be passed from the side of the blood inlet 7 1, 7, 2, 7 3 enter, and punching 404, 40 5, 40 6 (at least through the bottommost sheet) is the gas siphon outlet, and the conductive electrode 1 can be changed according to different blood inlet positions. 5, 16 design form (as shown in FIG. 7), and the chemical carrier 251 is applied to the working electrodes 161, 162, 163 of the first layer 11 and the reference electrodes 1 5 1 , 1 5 2, 15 3 The reaction zone is formed; according to the embodiment shown in the first figure, the same test piece 1 can be used for multiple inspection purposes. Moreover, the test piece in which the blood enters from the side can be designed as a multi-stacking embodiment (such as the eighth figure), wherein the liquid liquid inlet port is 71, 72, 73, 74, 75, 76, 77, 78, 79 is disposed on the side of the test piece 1 ,, and the punching holes 404, 40 5, 406 are the air outlets of the blood siphon, and according to the embodiment of the fourth figure, a drop of blood can be used for various purposes. The test strips tested. As described above, the same test can be carried out on the test piece of the mother section to a group of four or more groups.

1301888 - First Case No. 94110748 Next Day Correction ^__ V. Invention Description (9) As mentioned above, a test piece structure that can be used multiple times as a test piece, we also propose another suitable optical detection method for blood glucose concentration. An embodiment of a blood glucose tester (eg, ROCHE) is used, as shown in FIG. & IX, which can be provided with three reaction zones having chemical carriers μ, 92, and 93 on a test piece 90, and Two notches 94' are provided corresponding to the two sides between each two reaction zones to easily break the used reaction zone. As for the chemical carrier formulation used in the present invention, it is described in detail below. 0 scoop chemical carrier in the reaction zone, the composition of which comprises: 1·Hydroxypropyl methyl • el lui〇se, content: 〇· 4% 2·Citric acid, content: 0.096% 3·Dibasic potassium phosphate, content: 0 · 4 5 3 〇/〇4 · Nonionic surfactant τ ri ΐ ο η X - 1 0 〇, content: 〇· 〇 4 % 5. Glucose oxidase, content: 0.7% 6. Red blood salt (Potassium ferricyanide), content: 3. 5% 7 · Deionized water, content: 94.81% 8 · nm Carbon tube, content: 0. 05% _ where hydroxypropylmethyl cellulose is used for the first time, so that the chemical carrier can be evenly distributed when dried on the test piece; citric acid is used to adjust 2 Acid test, and it also has anticoagulant effect; dipotassium hydride is the second acid value; nonionic surfactant Triton X-100 is used for precipitation surface tension, which helps the dissolution of additives Glucose oxidase is low in electrochemical reaction with blood; red blood salt is used as conductive mediator ·Two μ μ, Nai Lai

Page 13

1301888 V. INSTRUCTIONS (10) Carbon tubes are also our first use to increase the reaction rate and reduce the reaction time as shown in Figure 10. Carbon nanotubes can be used or not; use of elements that reduce reaction time is not absolutely necessary. The aforementioned chemical carriers (251 to 253) are used on the working electrodes (161 to 163) and the test electrodes (151 to 153) in the punching holes (201 to 203) by a Musashi precision dispenser. The biologically active substance and the conductive medium of the carrier are adsorbed, and the electrode portion is changed from hydrophobic to hydrophilic, so as to facilitate the adhesion of the product to protect the biologically active substance from drying at a higher temperature. Deductions, while the biologically active substances described are only those that have been fixed or even not immobilized (such as glucose oxidase GUc〇se = idase), antigens, antibodies, microbial cells, animal and plant cells, animal and plant tissues. The biological identification ability of the component 'the biological tissue that is used to contact with the test sample 0 blood, etc.) produces a biochemical or chemical reaction; and the aforementioned conductive medium (such as Potassium ferricyanide, quin〇nes) content of 3 5%, is used The electrons generated after the reaction of the enzyme and the sample are received, and the electrons are transmitted to the sensor via the electrode wire to be converted into a sample concentration.

Page 14 1301888 Case No. 94110748 Simple explanation of the monthly correction diagram [Simple description of the illustration] The first figure is a three-dimensional combination diagram of the biological detection test piece which can be used repeatedly for the present invention. The three-dimensional exploded view of the bioassay test piece is a cross-sectional view of the bio-detection test piece which can be used multiple times in the present invention. The fourth figure is a piece of the present invention which can be used multiple times and one drop of blood can be tested for the same or different. The third figure of the bioassay test piece of the test item is a cross-sectional view of the bioassay test piece which can be used multiple times and one drop of blood can be tested for three identical or different test items. A three-dimensional combination diagram of a biodetection test piece which can be used multiple times and has a blood inlet side. The seventh figure of the present invention is a conductive electrode of a bioreactive test piece which can be used multiple times and has a blood inlet side. The embodiment is not intended to be a bioassay test of the present invention which can be used multiple times, the blood inlet is on the side, and one drop of blood can test three identical or different test items. FIG. 9 is a schematic view showing another embodiment of the present invention. FIG. 10 is a chemical reaction diagram of a chemical carrier having a carbon nanotube and a carbon nanotube without a carbon nanotube and a freehand electrochemical reaction diagram. 1 0, 9 0 sheet 11 , 1 2 , 1 3 , 1 4 small sheets 1 2 1 , 1 2 2, 1 2 3, 1 3 1 , 1 3 2 , 1 3 3, 1 41 , 1 4 2 , 1 4 3 punching 2 0 1 , 2 0 2 , 2 0 3

Page 15 1301888 Case No. 94110748 The monthly correction pattern simply describes the recesses 19, 191, 192, 193, 194, 195, 196 recesses 301, 302, 303 punches 401, 40 2, 403, 404, 4 05 >40 6 Chemical carrier 2 5 1 , 2 5 2, 2 5 3, 9 1 , 9 2, 9 3 Blood 4 0 Blood enters π 71, 72, 73, 74, 75, 76, 77, 78, 79 electrodes Head 51, 52, 53, 54, 55, 56 electrode lead 1 5, 16 6 working electrode 161, 162, 163 _ reference electrode 151, 152, 153 punching 1 7 , 1 8

Page 16

Claims (1)

1301888 The following is the revised date of April, W. The scope of application for patents Y is a multi-use bioassay test piece structure, mainly consisting of four layers of sheets, two electrode leads with two or more working electrodes and reference electrodes, And a chemical carrier placed in the reaction zone; the four-layered sheet comprises a bottom layer on which two electrode wires having two or more working electrodes and a reference electrode are attached, and two independent reaction zones are punched on the small-sized sheet. In the second layer, there are three small slices on the three small slices, and a third layer having three independent air outlets on the three small slices; and the two have more than two sets of working electrodes and reference electrodes The electrode wires must have the same resistance from the respective working electrode or reference electrode to the electrode tip. The multi-use bioassay test piece structure is mainly composed of three layers of sheets, two electrode wires having two or more working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone; a bottom layer of two electrode wires having two or more working electrodes and a reference electrode, a second layer having three independent blood siphon concaves on two small sheets, and three independent air outlets on the two small sheets The third layer; and the two strips have two or more electrode electrodes of the working electrode and the reference electrode, and the resistance of each working electrode or reference electrode to the electrode tip must be the same. 3 3. A bioassay test piece structure that can be used multiple times and one drop of blood can test three identical or different test items, mainly consisting of eight layers, eleven or sixteen layers, and four and six The strip or the eight strips are composed of two or more electrode wires of the working electrode and the reference electrode, and a chemical carrier placed in the reaction zone, and the eight layers, twelve layers or sixteen sounds or more are included. There are two bottom layers of the Dipole wire with two or more working electrodes and reference electrodes, and three independent reaction zones on the three small slices. The punching white ^ _1 No. 9411 Π 7 Μ Λ η η 曰 1301888 曰Amendment No. 94110748 /, Shen Qing patent range layer, there are three independent blood siphon concave third on the three slices: 佟i has three small slices on the fourth layer with three independent air outlets; == The electrode wires of the electrode and the reference electrode of the upper and lower electrodes must have the same resistance as the electrode or the reference electrode to the electrode tip. 4 The same household nfs can be used multiple times, and one drop of blood can be tested for three identical or non-phase =: test strip structure 'mainly consists of six layers, nine layers or presents;: wide and four, six or more and Each group of wires is packaged; six layers, nine layers or more than twelve layers = the bottom of the line; the electrode guides of the working electrode and the reference electrode above the group, and the two separate reaction areas are punched on the second two - one small piece Two ^, three small slices have three independent blood siphon concave small slices on the third layer of three independent air outlets; and;; thunder: =: electrode and reference electrode electrode wires, their work The resistance of the electrode or reference electrode to the electrode tip must be the same. 5. A bioassay test piece structure that can be used more or less and has a blood inlet on the side, mainly consisting of three layers of sheets, two sets of working electrodes and reference electrode wires, and a chemical carrier placed in the reaction zone. a layered sheet package with two or more working electrodes and a reference electrode attached thereto: a bottom layer of the electrode lead, three independent blood siphons, and three third layers: a vertical air outlet; and the two The second electrode is Li; the electrode wire of the drain electrode must have the same resistance of each working electrode or reference electrode to the electrode tip. ^ $ 电 6. - Can be used multiple times, blood inlet on the side, and - blood can be tested Page 18 1301888 --- Case No. 9411074R VI. The scope of the biometric test strips of the same or different test items, mainly consisting of six, nine or more layers, four, six or more Each set of wires has two or more sets of electrode wires of the working electrode and the reference electrode, and a chemical carrier placed in the reaction zone; six, nine or more layers of the slice comprise two or more layers of work The bottom layer of the electrode lead of the electrode and the reference electrode, the second layer having three independent blood siphon recesses, and the third layer having three independent gas outlets; and the two electrodes having two or more working electrodes and a reference electrode The resistance of each wire or its reference electrode to the electrode tip must be the same. The test piece construction according to any one of the preceding claims, wherein the substrate of the sheet may be a polyvinyl chloride (PVC) sheet or Glass fiber (FR-4) board, polyester (p〇iyester sulphone), electric board, PET board, PC board, glass board, ceramic magnetic board (CEM-1) 〇8·If you apply for the first or second patent scope The test piece structure according to any one of the items 3, 4, 5, and 6, wherein the substrate of the electrode wire may be a silver glue or a carbon glue wire in a screen printing process. 9) The test piece structure as described in any one of claims 1 or 2, 3, 4, 5, and 6 is in the chemical carrier component in each reaction zone of the test piece. ·Hydroxypropyl methy 1 ce 1 1 u 1 〇se, Cicritic acid, Dibasic potassium phosphate, nonionic surfactant Triton X- 100, Glucose oxidase, red blood salt (Potassium f err i cyan i de ), deionized water (De i on iz ed Page 19 1301888 __ Case No. 94110748_Yearly Month_Amendment___ VI. Patent Application Range • Water), Nanotube carbon. 10. The test piece structure according to item 8 of the patent application scope, wherein the electrode mold wire may also be a conductive reaction method by an additive reaction method; or may be a subtractive multiplication method ( The chemical reaction method of the Subtractive process removes the conductor which is partially useless on the surface; and then replaces the noble metal with a precious metal such as gold or platinum by a chemical immersion method (commonly known as gold) to replace the precious metal on the substrate. 11. The test piece structure of claim 9, wherein the chemical carrier comprises a multi-walled and single walled carbon nanotube having a size of 1 〇 to 2 0 0, The content is from 0.01% to 2%. 12. The test piece structure of claim 9, wherein the chemical carrier comprises Hydroxypropyl Methyl Cellulose in an amount of 5% to 2% by weight. 1 3 · A multi-use bioassay test strip structure comprising: a plurality of detection zones having chemical carriers disposed on the test piece, and a break gap between each of the two detection zones for use in The optical method detects the test article and reaches a blood glucose test piece that can be used multiple times per piece. Page 20 1301888 _ Case No. 94110748 VI. Designation of Representative Representatives (1) The representative figure of this case is: The second figure (2), the symbol of the symbol of the representative figure of this case is simple: Test piece 1 0, 9 0 Sheet 11 1 2 , 1 3 , 1 4 small sheets 121 , 122 , 123 , 131 , 132 , 133 , 141 , 142 , 143 punching holes 201 , 202 , 2 03 recesses 19 , 191 , 192 , 193 , 194 , 195 , 196 Concave 301, 302, 303 • Center hole 4 0 1 , 4 0 2, 4 0 3 Chemical carrier 251, 252, 253 Electrode head 51, 5 2 Electrode wire 1 5, 1 6 Working electrode 1 6 1 , 1 6 2 , 1 6 3 reference electrodes 151, 152, 153 punching holes 7 7 , 1 8 Page 3