TW200523540A - Structure for multi-use electrode testing sheet - Google Patents

Abstract

The present invention provides a structure for multi-use electrode testing sheet and the chemical carrier contained therein for use in bio-sensory devices to detect the components of the sample and its density based on the electrochemical effect generated by the sample in the biochemical reaction. The main structure comprises a plurality of thin sheets adhered together, and the biochemical reaction carrier is placed in the punched hole formed on the sheet surface, and the thin sheets can be easily broken and separated for another test. There is one layer of two positive and negative conductive electrodes between the sheets for conducting the reactive electrochemical signal, and the electrode wiring could be designed with the same resistance for each electrochemical reaction area, so that the testing sheet could be repetitively used. Other features of the present invention include: using one drop of blood for simultaneously measuring at least two same or different testing items. Moreover, adding carbon nanotubes in the chemical carrier could greatly speed up the biochemical reaction speed to reduce the testing time.

Description

200523540 V. Description of the invention (1) [Technical field to which the invention belongs] The present invention relates to a biochemical test strip for personal use, particularly a blood biochemical test strip that can be used multiple times with a single piece, and can be simultaneously used with one drop of blood. Measure two or more biochemical test strips with the same or different test items. [Prior technology] At present, all the blood glucose test strips on the market are test strips that can only be used once and thrown away after use. This kind of test strip is too expensive and unaffordable for people with diabetes in developing or undeveloped countries. It can not be regularly tested for blood glucose according to the condition, and even life-threatening. They desperately need a cheaper blood glucose test strip. At the same time, the current blood glucose test strips on the market are not accurate enough, and they are limited to the materials and methods they use, which often causes errors of more than 30%. Moreover, with the exception of a few European and American brands, it takes fifteen to thirty seconds to measure. Therefore, the main object of the present invention is to provide a blood glucose test strip which is cheaper, more accurate, and has a shorter measurement time. Another main object of the present invention is to provide a blood glucose test strip. As long as one drop of blood is taken, the pain can be measured once, and the same or different readings can be measured with one drop of blood. The purpose of reading the same sample is to be able to do more blood glucose tests and obtain an average value to increase accuracy, but it is not necessary to take more blood to reduce the pain of blood collection; and one drop of @ 血 can measure several different The purpose of reading the sample is to be able to test different items at the same time, but it is not necessary to take more blood to reduce the pain of blood collection. So far, no patent in the world has been filed in a single

Page 5 200523540 V. Description of the invention (2) The structural design and method that can be used repeatedly on the biochemical test strips that are disposable and thrown away to reduce the financial burden on users. Our previous patent application (U.S. Patent Application No. 0.10 / 704,701) first proposed an electrochemical test strip that can be used multiple times on a single test strip, but the content is the same as this application different. 3 乜 1]: US Patent No. 5,120,420 filed by 〇1 ^ 111 ^ 1 et al. Discloses a method in which a plurality of electrode leads are provided on a test strip, so as to measure the blood glucose concentration of several samples at the same time, and then take the average value. To increase accuracy. However, this method requires a relatively large amount of blood, which is not feasible for patients who self-test at home ^. At the same time, this method does not help reduce the user's inspection costs. In addition, there are many research reports and patents suggesting various methods to improve the accuracy or speed of measurement. For example, U.S. Patent No. 6, 4 91, 803 of T h mas Y · S · S he η mentions the use of nanometer metal powder to increase the conductivity and reduce the reaction time, but it still takes fifteen seconds. measure time. Har t (Biosensors & Bioelectronics, 13: 333-339, 1996) ^ Now adding platinum powder on the electrode surface can increase the conductivity to improve the measurement accuracy, but these effects are not good enough. C. D. Wisley et al., U.S. Patent No. 6,5 4 1, 2 1 6 pointed out that the use of plasma treatment of the surface of the silver electrode and the carbon electrode can increase the accuracy of the test strip measurement. However, the above methods cannot achieve the goals of cheaper, more accurate, and faster measurement speed required by this patent, nor are they the methods adopted by this patent. [Summary of the Invention] In order to achieve the above-mentioned object, the present invention provides a product that can be used multiple times.

Page 6 200523540 V. Description of the invention (3) Chemical test strips, which mainly include the structure of a disposable, disposable electrode strip that can be used multiple times, and the chemical carrier contained in it. It is used in conjunction with a biosensor detection device, and detects the components of the sample and determines its concentration according to the electrical effect of the sample in the biochemical reaction. The structure of the present invention is formed by pasting four layers of flakes, and the space formed by various punching holes on the flakes can generate three identical reaction zones, and a biochemical reaction carrier is placed on each reaction zone. Only 2 # are required. The amount of blood in 1 is based on the principle of siphoning. After being sucked into the reaction zone, blood will electrochemically react with the biochemical reaction carrier thereon, and the blood glucose of the blood test substance can be obtained by analyzing the result of the electrochemical reaction. concentration. At the same time, the sheet is formed by various punching holes. The space can make the test piece easily break the used test piece due to the stress concentration factor, so as to reuse the other unused parts for the second or third time. There are two positive and negative conductive electrodes on the bottom sheet to conduct the electrochemical signal of the reaction, and the electrode lead is designed so that the positive and negative electrodes of each electrochemical reaction zone and the positive and negative electrodes on the stem electrode tip have the same resistance> Having the same resistance is the key factor that the test strip disclosed by the present invention can be used multiple times, and it is also an indispensable element. This method is adopted for the first time in the world. The positive and negative electrodes of each electrochemical reaction zone will transmit the electrochemical reaction signal generated by the reaction between the chemical carrier on it and the blood to the positive and negative electrodes on the measuring electrode head, and then the blood glucose reading of the blood test product can be judged by the blood glucose tester. . As for the formula of the chemically reactive substance that we have developed exclusively, it contains hydroxypropyl methyl cellulose to distribute the chemically reactive substance evenly. This is the same as that used in US Patent 5,120,420. Carboxymethyl cellulose, and the Chinese people

Page 7 200523540 V. Description of the invention (4) National patent 3 7 4 1 1 6 cellulose (Microc world we first adopted in addition to single walled), it can reverse the seconds, and can provide solid, The test strips revealed by this method can give accurate results. § Furthermore, if the same four layers or eight drops of blood can be zoned, the same or two measurements can be taken at the same time to measure more than two kinds of accuracy; but if the time is exceeded, the data can be reduced, and each time The shortest functional requirements used for testing 1 [Implementation] In order to make your first-level understanding and use in the United States special rysta 1 1 i. We also add nano carbon tubes to the appropriate table from time to time. We repeat the above layers multiple times, the same drop on different siphons costs four layers per square meter, but the same test article J & L is the same. In other words, it is different from the ultra-fine particle cellulose used in Lili 6, 4 1 3, 3 9 4 B 1, which is also a multi-walled and single-walled (multi-walled and multi-walled) to increase the conductivity and reduce the general three Decrease ten seconds to four seconds or even two-sided tension and surface energy to prevent blood clotting from being used for the first time. In this way, the test piece used by the present invention can quickly obtain a test piece formed on the top layer and then add a siphon. The blood injection positions are all in the same position, so one or three different, independent reaction reactions are detected, so one drop of blood can be used or different samples. If one drop of blood is simultaneously, the average value can be taken to increase its on-time measurement. There are two or more different types of pain in blood. Blood glucose test strips can reduce the user's accuracy even when the test time is the highest. Characterized to have deeper and its possible embodiments are explained in detail with FIG.

Page 8 200523540 V. Description of the invention (5) The description is as follows: Please refer to the first figure, the second figure and the third figure. The multi-use bio-assay test strip 10 according to the present invention is composed of four Layers 1 1, 1 2, 1 3, 1 4 are pasted, and two leads 15, 16 are placed between the sheets 1 1, 12, the leads 15 are connected to three reference electrodes 151, 152, 153, The lead 16 is connected to the three working electrodes 1 6 1, 1, 6 2, 1 6 3 (as shown in the second picture). 0 There are two punch holes 1 7 and 1 8 on the sheet 1 1, which can generate stress concentration to facilitate testing. The sheet is broken, and another indentation 19 is formed on the sheet 11 to indicate the blood inlet position. _ Sheet 1 2 is divided into three small sheets 1 2 1, 1 2 2, 1 2 3 attached to the wires 1 5, 16 and sheet 1 1; the three small sheets 1 21, 1 2 2, 1 2 3 are in the middle The gap is to allow the combined test piece to be broken (such as the third figure C), and the three small sheets 1 2 1, 1 2 2, 1 2 3 each have three punching holes 2 0 1, 2 0 2 2 0 3 to place the chemical carrier 251, 2 5 2, 2 5 3 on the working electrodes 161, 162, 163 and the reference electrodes 151, 152, 153 (as shown in the third figure A) to become three different. The reaction zone, And at the ends of the three small sheets 1 2 1, 1 2 2, 1 2 3, an indentation 1 9 1, 1 9 2, 1 9 3 is provided to mark the blood inlet position. The sheet 1 3 is divided into three small sheets 1 3 1, 1 3 2, 1 3 3 and affixed to the sheet 1 2. Similarly, there is a gap between the three small sheets 1 3 1, 1 3 2, and 1 3 3. In order to break the combined test piece well, and it has three indentations 3 0 1, 0, 3 0 3 to allow blood 40 to inhale electrochemical reactions with chemical carriers 251, 252, and 253 through the principle of siphon. (As in the third picture A and B). Therefore, the test strip only requires 2 // 1 blood volume per test.

Page 9 200523540 V. Explanation of the invention (6) The blood will be sucked into the reaction zone and then it will electrochemically react with the biochemical carrier on it, and the blood glucose of the blood test substance can be obtained by analyzing the result of the electrochemical reaction. concentration. The sheet 14 is divided into three small sheets 1 4 1, 1 4 2, and 1 4 3 attached to the sheet 1 3 with three indentations 194, 195, and 196 on it to indicate the location of blood infusion; The holes 401, 402, and 403 are used as the air outlets for the blood of the siphon principle. The conductive electrodes 15 and 16 are designed as reference electrodes 1 5 1, 1 5 2, 1 5 3 and electrode head 5 1 with the same wire length, that is, the same resistance, and the working electrodes 1 6 1, 1 6 2, 1 6 3 to the electrode tip 5 2 need to have the same lead length, degree, that is, the same resistance, so that the biochemical reaction results in these three different reaction areas will be consistent. As mentioned above, the same resistance of each of the conductive electrodes 15 and 16 is the key factor that the test strip disclosed by the present invention can be used multiple times, and it is also an indispensable element. This method is used for the first time in the world. In the structure described above, the sheets 1 1, 1 2, 1 3, 1 4 can be made of plastic, ceramic substrate, cardboard or FRP (glass fiber reinforced plastic); and the conductive electrodes 1 5 and 16 can be screen-printed. The silver or carbon glue wire of the printing process can also be used to generate the required conductive circuit by the Additive Process and the chemical reaction method; it can also be used the Subtractive Process and the chemical reaction method to Locally useless conductors are removed on the surface; then precious metals such as gold, platinum and other precious metals are replaced by chemical immersion plating (commonly known as gold), and the precious metals are replaced on the substrate with a chemical substitution reaction. In the mass production of more than 100 pieces, the thickness of the precious metal can be averaged, that is, the resistance of the three reaction zones on each piece can be

Page 10 200523540 V. Description of the invention (7) The error maintained within 1%. Our method is different from U.S. Patent 5,437,999 and Republic of China Patent 496110, because the latter is plated with precious metals by electroplating. All test pieces need to be connected to the same electrode (our method does not need), which will cause The thickness of the noble metal plating on the test piece is uneven, and the resistance in the three reaction zones exceeds an error of more than 30%, so the error is too large to be applicable. When the structure is used as described above, please refer to the third figures A to D to show the use process of the present invention when the blood 40 enters the test strip 10. After the blood 40 enters the recessed area 3 0 1 on the right end of the test strip 1 10 through the siphon effect and fills the reaction area in the punching hole 2 0 (third picture A to B), wait for a few seconds for the blood glucose reading After B appears, the test piece of the used first reaction zone can be broken (such as the third picture C), and the unused second reaction zone (such as the third picture D) is exposed, and then the next step can be performed. A blood test. By analogy, after the second reaction zone is used up, the test piece of the second reaction zone can be broken to expose the third unreacted reaction zone, and then the test piece 10 can be subjected to the first Three blood tests are used to achieve the purpose of multiple use in the same test strip. Please refer to FIG. 4 and FIG. 5 again, which shows three embodiments of the same structure but different lengths of the test strips 10 shown in FIG. The entrance position of the punching hole 3 0 1 is the same, and the punching holes 4 0 4, 4 0 5 and 4 0 6 which are the air outlets are at least penetrated from the top to the bottom sheet, and three identical ones can be made with one drop of blood at the same time. Or detection of different items. When this embodiment is used, as shown in the fifth figure, when a drop of blood 40 is inhaled and fills the three reaction zones a, b, and

Page 11 200523540 V. Description of the invention (8) c. The chemical carrier 251 in each reaction zone will electrochemically react with the blood 40 drawn into each reaction zone at the same time. Among them, the chemical carriers 251 in the three reaction zones a, b, and c may be the same type to generate the same reading value, and then take the average value to obtain better accuracy; it may also be a different type with one drop Blood gets three different readings. In the same way, this kind of test piece can be broken and used again after the first test. The structure of the test piece described above can also be changed. As shown in the sixth and seventh figures, the test piece 10 is designed as a three-layer sheet 1 1, 1 2, 1 as shown in the sixth figure. 3 Adhesive, in which the recessed blood inlets 7 1, 7 2, 73 are provided on the second sheet 12 and are all designed on the other side of the electrode heads 51, 2 in the extension direction, so that the blood must be from the side blood The inlets 7 1, 7 2, 7 3 enter, and the punching holes 404, 405, 406 (at least through the bottommost slice) are the air outlet for blood siphon, and the conductive electrodes can be changed according to different blood inlet positions 1 5 And 16 design patterns (as shown in Figure 7), and the chemical carrier 251 is applied to the working electrodes 161, 162, 163 and reference electrodes 1 5 1, 1 5 2, 1 5 3 of the first layer 11 According to the formed reaction zone, as shown in the embodiment of the first figure, the purpose of using the same test strip 10 for multiple tests can be achieved. In addition, this type of test piece with blood entering from the side can also be designed as a multi-stacked embodiment (such as the eighth figure), in which the blood inlets 71, 7 2, 7 3, 7 4, 7 5, 7 6, 7 7, 7 8 and 7 9 are obtained and set on the side of the speech sheet 1 0, and the punching holes 4 0 4, 4 5 5 and 4 0 6 are the outlets of blood siphon. According to > the same can be achieved as As shown in the example of the fourth figure, a single drop of blood can be used for a variety of tests. As mentioned above, the test pieces in each section can be further stacked into four or more groups of reaction zones.

Page 12 200523540 V. Description of the invention (9) As mentioned above, a test strip structure can be used multiple times. We also propose another blood glucose tester (such as: R 0 C Η E) An embodiment of the design used, as shown in FIG. 9, it can be provided on a test piece 90 with three reaction zones with chemical plants 91, 92, 93, and in every two reaction zones Two gaps 9 4 are provided correspondingly between the two sides to easily break the used reaction zone. As for the chemical carrier formulation used in the present invention, the details are as follows. The chemical carrier in the reaction zone has a composition of: 1. Hydroxypropyl methyl cellulose, content: 0.4 ° / 〇_, Citric acid, content: 0.096% 3 · Dibasic potassium phosphate, content: 0.453% 4 · Non-ionic surfactant T rit ο η X-1 0 0, content: 〇 · 〇4% 5 · Glucose oxidase (Glucose oxidase ), Content: 0.7% 6. Red blood salt (Potassiumferricyanide), content: 3.5% 7. Deionized water, content: 94.81% ^ Nanotube carbon, content : 0.05% Among them, we used hypromellose cellulose for the first time to make the chemical plant evenly distributed when dried on the test strip; citric acid is used to adjust the pH value, and it also has anticoagulant Role; dipotassium hydrogen phosphate is used to adjust the acid test value; non-ionic surfactant T rit ο η X-1 0 〇 is used to reduce the surface tension of water and help the dissolution of additives; glucose oxidase is Will react electrochemically with the blood Potassium ferricyanide is used as a conductive medium; nm

Page 13 200523540 V. Description of the invention (10) The carbon tube is also our first use to increase the reaction rate and reduce the reaction time, as shown in Figure 10. Nano carbon tubes can be used or not; the use of them can reduce the reaction time and is not an absolutely necessary element. The aforementioned chemical carrier (25 1 to 2 5 3) is a working electrode (161 to 163) and a reference electrode (1 5 1) that are placed in a punching hole (201 to 2 0 3) by a Musashi precision dispenser. ~ 1 5 3), to adsorb the bioactive substance and conductive medium of the carrier, and change the electrode part from hydrophobic to hydrophilic to facilitate the adhesion of the test product, and further protect the bioactive substance from The high-temperature drying process will not be damaged; the aforementioned bioactive substances only include immobilized or even unimmobilized enzymes (such as glucose oxidase GiUCC) se X idase), antigens, antibodies, microbial cells, animal and plant cells 2. The components of animal and plant tissues with biological identification ability, which are used to produce biochemical or chemical reactions with the test objects (such as blood and other biological tissues) in contact with it; and the aforementioned conductive medium (such as Potassium ferricyanide, quinones) content is 35%, It is used to receive the electrons generated after the enzyme reacts with the sample, and then pass this electron to the sensor via the electrode wire to convert it into the sample concentration.

Page 14 200523540 Brief description of the drawings [Simplified illustration of the diagram] The first picture is a three-dimensional combination diagram of the bio-detection test strip that can be used multiple times according to the present invention. Exploded view. The third figure is a cross-sectional view of a bio-assay test strip that can be used multiple times according to the present invention. The fourth picture is a bio-test strip that can be used multiple times and one drop of blood can test three same or different test items. The fifth figure is a cross-sectional view of a bio-test strip that can be used multiple times and one drop of blood can test three same or different detection items. A three-dimensional combined view of a biological test strip with a blood inlet on the side _ The seventh figure is a conductive electrode in a biological test strip with a blood inlet on the side that can be used multiple times in the present invention This is a three-dimensional combination diagram of a biological test strip that can be used multiple times, the blood inlet is on the side, and one drop of blood can test three same or different detection items. The ninth figure is The schematic diagram of another embodiment of the invention. The tenth figure is a chemical carrier with and without a carbon nanotube and a free electrochemical reaction chart. [Description of the main component symbols] Test piece 1 0, 9 0 ⑩ sheet 1 1 , 1 2, 1 3, 14 Small sheet 1 2 1, 1 2 2, 1 2 3, 1 3 1, 1 3 2, 1 3 3, 1 4 1, 1 4 2, 1 4 3 Punching 2 0 1 , 2 0 2, 2 0 3

Page 15 200523540 Schematic description of recessed 19, 191, 192, 193, 194, 195, 196 recessed 301, 302, 303 Punch L 4 0 1, 4 0 2, 4 0 3, 4 0 4, 4 0 5, 4 0 6 4 Carrier 251 > 2 5 2 > 2 5 3, 91, 92, 93 Blood 4 0 Blood inlets 71, 72, 73, 74, 75, 76, 77, 78, 79 Electrode heads 51, 52, 53, 54, 55, 56 Electrode leads 1 5, 1 6 Working electrodes 161, 162, 163 Reference electrodes 151, 152, 153 _ punched holes 1 7, 1 8

Page 16

Claims (1)

200523540 6. Scope of patent application 1. A multi-use bio-detection test strip structure, which is mainly composed of four layers of thin films, two electrode wires with two or more sets of working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone. It consists of a four-layer sheet with a bottom layer with two electrode wires with two or more sets of working electrodes and reference electrodes, a second layer with three small sheets with three independent reaction zone punches, and three with three small sheets. Independent third layer of blood siphon with inner concave and fourth layer with three independent air outlets on three small sheets; and the two electrode wires with two or more sets of working electrodes and reference electrodes' each working electrode or reference The electrode-to-electrode tip set must be the same. 2. A reusable biological test strip structure, which is mainly composed of three layers of thin sheets, two electrode wires with two or more sets of working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone; A bottom layer comprising two electrode wires with two or more sets of working electrodes and reference electrodes attached thereto, a second layer with three independent blood siphon recesses on three small sheets, and three independent air outlets on three small sheets The third layer; and the two electrode wires having more than two sets of working electrodes and reference electrodes, the working groups of each working electrode or reference electrode to the electrode tip must be the same. 3. — A kind of biological test strip structure that can be used multiple times and one drop of blood can test three kinds of the same or different test items, mainly composed of eight, twelve or sixteen or more layers, and four, six Consisting of electrode wires of more than eight working wires and each set of wires having more than two working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone; There are two bottom layers of electrode leads with more than two sets of electrodes and reference electrodes, and three small sheets with three independent reaction areas punched. Page 17 200523540 6. The second layer of the scope of patent application, the third layer with three independent blood siphon indentations on three small sheets, and the fourth layer with three independent air outlets on three small sheets; and the two There are more than two sets of working electrodes and reference electrodes. The working groups of each working electrode or reference electrode to the electrode tip must be the same. 4 · The structure of a biological test strip that can be used multiple times and one drop of blood can test three same or different test items, mainly composed of six, nine or twelve or more layers, and four, six or Eight or more sets of wires each having two or more sets of working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone; six, nine, or twelve or more sheets include two The bottom layer of the electrode guide wire of the two or more sets of working electrodes and reference electrodes, the second layer with three independent reaction zone punches on three small sheets, the third layer with three independent blood siphoning recesses on three small sheets, And the fourth layer with three independent air outlets on three small sheets; and the two electrode wires with more than two sets of working electrodes and reference electrodes, each working electrode or reference electrode to electrode tip must be the same. 5. — A kind of bio-detection test strip structure that can be used multiple times and the blood inlet is on the side. It is mainly composed of three thin sheets, two electrode wires with two or more sets of working electrodes and reference electrodes, and a chemical placed in the reaction zone. The carrier consists of a three-layer sheet including a bottom layer with two electrode wires with two or more sets of working electrodes and reference electrodes, a second layer with three independent blood siphon recesses, and a first layer with three independent air outlets. Three layers; and the two electrode wires that have more than two sets of working electrodes and working electrodes, the working groups of each working electrode or reference electrode to the electrode tip must be the same. 6 · — a type that can be used multiple times, the blood inlet is on the side, and a drop of blood can be tested Page 18, 200523540 VI. Patent application structure Three types of biological test strips with the same or different test items are mainly composed of six, nine or twelve or more layers, four, six or eight or more and each group The lead wire is composed of more than two sets of working electrodes and reference electrodes, and a chemical carrier placed in the reaction zone; six, nine, or twelve or more layers of sheets include two working electrodes with two or more sets of electrodes and The bottom layer of the electrode lead of the reference electrode, the second layer with three independent blood siphon recesses, and the third layer with three independent air outlets; and the two electrode wires with two or more sets of working electrodes and reference electrodes, The working group of each working electrode or reference electrode to the electrode tip must be the same. 7 · The test piece according to item 1 or any of items 2, 3, 4, 5, 6 in the scope of patent application, wherein the substrate of the sheet may be a polyvinyl chloride (PVC) board or glass Fiber (FR-4) board, polyester su 1 ph ο ne, bakelite board, PET board, PC board, glass board, ceramic magnetic board (CEM-1) ° 8. If item 1 of the scope of patent application or The test strip according to any one of items 2, 3, 4, 5, and 6, wherein the base material of the electrode lead may be a silver or carbon gel lead in a screen printing process. 9. According to the test strip described in item 1 or any of items 2, 3, 4, 5, 5 and 6, the chemical carrier component in each reaction zone of the test strip contains: Hydroxypropyl methyl cellulose, Citric acid, Dibasicpotassiumphosph ate, Triton X-100, a non-ionic surfactant, and Glucose oxidase ·· Potassium ferricyanide, Deionized water Page 19 200523540 VI. Patent application scope Water), Nano carbon carbon (Nanotube carbon). 10. The test strip as described in item 8 of the scope of patent application, wherein the electrode lead can also be used to generate the required conductive circuit by the chemical reaction method of the Additive Process; it can also be the subtractive process. The chemical reaction method removes partially useless conductors on the surface; then, the precious metals such as gold and platinum are replaced by chemical dip plating (commonly known as gold), and the precious metals are replaced on the substrate by a chemical substitution reaction. 11. The test strip as described in item 9 of the scope of patent application, wherein the chemical carrier contains multi-walled and single-walled (multi-walled and single walled) carbon nanotubes, the size of which is 10 // m ~ 200 and the content is 0.01% ~ 2%. _ 2 · The test strip as described in item 9 of the scope of the patent application, wherein the chemical carrier contains Hydroxypropyl methyl cellulose, the content of which is from 0.5% to 2%. 1 3 · —A kind of multi-use bio-detection test strip structure. The test strip can be provided with several detection areas with a chemical carrier, and a break gap can be provided between each two detection areas, so that it can be used for The optical method is used to detect the test product, and each blood glucose test strip can be used multiple times. Page 20