TWI291327B - Method for fixing seaweed - Google Patents

Method for fixing seaweed Download PDF

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Publication number
TWI291327B
TWI291327B TW095117700A TW95117700A TWI291327B TW I291327 B TWI291327 B TW I291327B TW 095117700 A TW095117700 A TW 095117700A TW 95117700 A TW95117700 A TW 95117700A TW I291327 B TWI291327 B TW I291327B
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TW
Taiwan
Prior art keywords
seaweed
algae
fixing
germ cells
fixing method
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TW095117700A
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Chinese (zh)
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TW200743438A (en
Inventor
Yan-Chang Chen
Chia-Chieh Chen
Guan-Yin Chen
Shiue-Shiuan Liou
Bin Lin
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Atomic Energy Council
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

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  • Cultivation Of Seaweed (AREA)

Abstract

Provided is a method for fixing seaweed, wherein seaweeds (for example, wild ulva) are first collected and then an artificial is employed to use different osmotic pressures (salt degree) to stimulate the seaweeds to release suspended germ cells, and the germ cells are allowed to proceed to adhere to achieve the artificial culture purpose of fixation and quantification. It is helpful to produce algae in the future and provides a great quantity of source of biomass energy materials.

Description

1291327 九、發明說明: 【發明所屬之技術領域】 本發明是有關於一種海藻固定方法,尤指將野生 巨型海澡釋放出的懸浮性生殖細胞進行固定化與量化 之方法,以達到人工培養的目的。 【先前技#f】 • 按,能源與我們曰常生活息息相關,舉凡食、衣、 住、仃、育、樂皆與能源脫離不了關係。隨著工商業 的繁榮與物質文明的高度發展,人類正面臨著巨大的 能源與環境壓力。1291327 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a method for immobilizing seaweed, and more particularly to a method for immobilizing and quantifying suspended germ cells released from a wild giant sea bath to achieve artificial culture. purpose. [Previous technology #f] • According to the energy, we are closely related to our daily life. The food, clothing, housing, living, education and music are all related to energy. With the prosperity of industry and commerce and the high development of material civilization, human beings are facing enormous energy and environmental pressures.

别所有能量來源中,佔有最大比例者為:煤炭 石油和天然氣···等的石化燃料,但石化燃料具有一 的儲存量,燃料的生成速度慢,醞釀期長,但是使; 速度快,更加速石化能源的使用殆盡。而大量使用』 化燃料容易產生溫室氣體,造成酸雨,且衍生全球_ 候日友化等問題’並可能面臨自然資源耗盡的一天。才! 據禹國能源部能源資訊署預估’在各項初級能源中, 石油可用40年,_妒妥妙名叮m〜> 般天;乳可用60年,煤炭可用莽 =原子能的轴可用七十多年。目前一般大果 薄弱’因此改變民幕的使用習慣和宣導與 源及再d 方面積極發展出替代能 再生此源,乃當務之急。 1291327 由於台灣缺乏石化能源,而核能源之前途有其發 :瓶頸。面對需求日益擴大之民生與產業能源,再生 月匕源之開發與研究已成為迫切需要之主題。舉凡太陽 能、㈣、風力、潮沙等再生及替代性能源之研發% 積極著手進仃,但面臨客觀環境條件不足及開發成 ,居高不下之嚴峻挑戰’生質能源的研究與利用,便 ^深具前瞻性的新思考方向。地球上的生物資源極 為豆富,據估計,地球每年經光合作用產生的生物質 )、勺有1,725 噸。作為一種可再生的能源資源,這 ^生物質擁有的能量相當於世界能源總消費量的 1〇〜2〇倍,但目前利用率很低,僅只有1%〜3%。因此 研發應用與能量轉換技術,以便能充分利用此類生物 質能,將可作為取代石化能源的最佳途徑。因此海藻 即為-種生質能材料,所以想以生質能取代石化能 源,必須大量開發海藻之產量,而目前大型藻類多以 野生為主纟生長地點為沿海一帶,固著於石塊岩礁 等地,品質及產量受到天候、氣溫及區域的限制,故 必須研:-翻定化及量化之人工培育海澡之方法, 如此大置提供高品質藻類以利新能源之開發。 【發明内容】 本發明之主要目的係在於,可人工培育海藻,利 用不同滲透壓(塩度)刺激其釋放出懸浮性的生殖細胞 後,讓生殖細胞進行附著,以達到固定化與量化,有 7Among the other sources of energy, the largest proportion is: fossil fuels such as coal oil and natural gas, but petrochemical fuel has a storage capacity, the fuel generation rate is slow, the brewing period is long, but the speed is faster. Accelerate the use of petrochemical energy. The large-scale use of chemical fuels is prone to greenhouse gases, causing acid rain, and deriving problems such as global rations and may face a day when natural resources are exhausted. According to the Energy Information Administration of the Ministry of Energy of the Republic of China, it is estimated that in all primary energy sources, oil can be used for 40 years, _ 妒 妒 叮 〜 〜 〜 〜 ; ; ; ; ; ; ; ; ; ; ; ; ; ;; Available for more than seventy years. At present, the general situation is weak. Therefore, it is imperative to change the use habits of the people's curtains and to actively develop alternative sources of regeneration and source and re-d. 1291327 Because of the lack of petrochemical energy in Taiwan, nuclear energy has its own path: bottlenecks. In the face of the increasing demand for people's livelihood and industrial energy, the development and research of the revitalization of the moon has become an urgent need. The research and development of renewable energy and alternative energy sources such as solar energy, (four), wind power, and tidal sand are actively carried out, but facing the serious environmental conditions and development, and the serious challenge of 'high quality energy' research and utilization, ^ A forward-looking new direction of thinking. The biological resources on the earth are extremely rich, and it is estimated that there is 1,725 tons of biomass produced by photosynthesis on Earth every year. As a renewable energy resource, this biomass has energy equivalent to 1 to 2 times the total energy consumption of the world, but the current utilization rate is very low, only 1% to 3%. Therefore, the development of applications and energy conversion technologies to make full use of this type of biomass energy will be the best way to replace petrochemical energy. Therefore, seaweed is a kind of biomass energy material. Therefore, in order to replace petrochemical energy with biomass energy, it is necessary to develop a large amount of seaweed production. At present, large algae are mostly wild, and the growth site is coastal area, which is fixed on the rocky reef. In other places, quality and production are limited by weather, temperature and region. Therefore, it is necessary to study: - the method of artificially cultivating sea baths by gradualization and quantification, so as to provide high-quality algae for the development of new energy. SUMMARY OF THE INVENTION The main object of the present invention is to artificially cultivate seaweed, stimulate the release of suspended germ cells by using different osmotic pressures, and let the germ cells adhere to achieve immobilization and quantification. 7

Claims (1)

1291327 愤Ί 曰1291327 Resentful 曰 '申請專利範圍: 一種海藻固定方法,係至少包括下列步驟: a•製備一海藻培養液; b.設置-具海藻附著器之培養皿,並進行滅菌後,倒 入海藻培養液,其中該海藻附著器係由—不錄鋼架 及-條綿繩組成’且由該綿繩纏繞於該不錄鋼架 上; /、 c•採集海藻置於一盛有海水之容器; d·利用滲透壓(塩度)刺激海藻放出生殖細胞,並將該 生殖細胞置入具海藻附著器之培養皿内,再將該培 養皿置入一植物培養箱,使生殖細胞附著於海藻附 著器;以及 e•待生殖細胞萌芽成海藻幼體,將海藻幼體置入一盛 有海藻培養液之三角燒瓶。 2·依申請專利範圍第1項所述之海藻固定方法,其中, 該海藻係可為石蓴。 、 依申叫專利範圍第1項所述之海藻固定方法,其中, 該海藻培養液之製備過程係取已滅菌海水,海水鹽 度3·5%,每升海水加入1ml之飽和氧化鍺水溶液。 4·依申凊專利範圍第1項所述之海藻固定方法,其中, 泫步驟b之滅菌方式係採用熱濕滅菌法。 5·依申凊專利範圍第1項所述之海藻固定方法,其中, 該步驟d之海藻係經光照射產生生殖細胞,該生殖'Scope of application: A method for fixing seaweed, comprising at least the following steps: a. preparing a seaweed culture solution; b. setting a culture dish with a seaweed attachment device, and sterilizing, then pouring the seaweed culture solution, wherein the seaweed The applicator is composed of - no steel frame and - a cotton rope and is wound on the unrecorded steel frame by the cotton rope; /, c; collecting seaweed in a container containing sea water; d · using osmotic pressure (twisting) stimulate the seaweed to release the germ cells, and place the germ cells in a petri dish with a seaweed attachment device, and then place the culture dish in a plant incubator to attach the germ cells to the seaweed attachment; and e• When the germ cells are germinated into seaweed larvae, the seaweed larvae are placed in a conical flask containing seaweed culture solution. 2. The method for fixing seaweed according to claim 1, wherein the seaweed system is sarcophagus. The seaweed fixing method according to the first aspect of the invention, wherein the seaweed culture liquid is prepared by sterilizing seawater, having a seawater salinity of 3.5%, and adding 1 ml of a saturated aqueous cerium oxide solution per liter of seawater. The seaweed fixing method according to the above aspect of the invention, wherein the sterilization method of the step b is a heat and humidity sterilization method. The seaweed fixing method according to the first aspect of the invention, wherein the algae in the step d is irradiated with light to produce a germ cell, the reproduction 1291327 細胞浮於該容器之海水表面。 6.依申請專利範圍第1項所述之海藻固定方法,其中, 該步驟d之植物培養箱之培養條件係為溫度介於20 r 至30度之間,光度介於145至155// ΕπΤΥ1之間, 及光週期為12/12小時(光/暗)。 7. 依申請專利範圍第1項所述之海藻固定方法,其中, 該三角燒瓶之容量係為50ml,並盛有40ml之海藻 培養液。 8. 依申請專利範圍第5項所述之海藻固定方法,其中, 該光照射時間係介於1至2小時。1291327 Cells float on the surface of the seawater of the container. 6. The algae fixing method according to claim 1, wherein the culture condition of the plant incubator of the step d is between 20 r and 30 degrees, and the luminosity is between 145 and 155 / / Ε π ΤΥ 1 The photo period is between 12/12 hours (light/dark). 7. The algae fixing method according to claim 1, wherein the flask has a capacity of 50 ml and contains 40 ml of an algae culture solution. 8. The algae fixing method according to claim 5, wherein the light irradiation time is between 1 and 2 hours.
TW095117700A 2006-05-18 2006-05-18 Method for fixing seaweed TWI291327B (en)

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