TWI287434B - Method for producing germinated seeds - Google Patents

Abstract

The present invention is related to a method for producing germinated seeds comprising germinating seeds by an acidic solution. The present invention provides a method for preparing a lower microbial count, a higher germination ratio, and a less off-flavor germinated seeds.

Description

1287434 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a method for germination treatment, and more particularly to a method for producing germinated seeds having low microbial contamination and no odor of water by utilizing electrolytic acidic water germination. [Prior Art] Rice is the main food crop in Taiwan, and rice is the mainstay of the Chinese people. In recent years, due to the pursuit of health and newspapers and magazines, the requirements of the food for the Chinese people, in addition to the appearance and taste, The pursuit of eating nutrition has led to the emergence of many brown rice products on the market. However, even if the ancestors of the literature have more nutrients than white rice, such as hemicellulose, vitamin E, inositol and rice sterol, Rice tastes better, so the Chinese still use more people who eat white rice. On the other hand, since joining WT, Southeast Asian rice has also been sold in the market, which has caused considerable impact on China's rice agriculture and market, so To increase the competitiveness of China's rice market and take into account the health of Chinese people, it is necessary to develop products that are more functional and nutritious than ordinary brown rice. After germination of brown rice, in addition to the various nutrients contained in brown rice, macromolecules such as proteins and sugars are degraded to a more digestible and absorbing sugar, amino acid and other small molecular substances by germination-driven biochemical metabolism. After the germination of brown rice, the content of a functional one is *_gaba (7-aminobutyric acid, Gamma-amin〇bUtyric acid) is greatly increased, because GABA has been confirmed to have a stable spirit, improve insomnia, lower blood pressure It promotes the blood circulation of the uterus and ovaries, reduces menopausal disorders, and reduces the health effects of aging symptoms. In modern Taiwanese society, life is tense and the number of silver-haired people increases. 4 1287434 Preferably, the acidic water system produces 0. More preferably, The acidic water system is produced by nature. Preferably, the acid water system is more preferably produced by the method of adding an acid organic acid to an aqueous solution of an aqueous solution of an electrolytic solution containing an aqueous solution of an electrolytic solution. Add acetic acid, citric acid, malic acid or

Preferably, the acidic water is from pH 2.3 to pH 6.5. More preferably, the acidic water is pH 3 〇 5 〇 Preferably, the seed is peanut, mung bean, soybean or brown rice. A germinated seed obtained by the above method. The present invention is further related to a sub-zero. From the above, the germination treatment method provided by the present invention can be understood, and the production of germinated seeds using electrolytic acidic water can effectively reduce the growth. The number of bacteria y; in addition to soaking water odor, thereby increasing the germination rate, and avoiding the possibility of using chemical agents, such as bleach; thus establishing a germinating seed that is more hygienic and of better quality than the traditional germinated seed production process. The product, further, can be found from the results of the test, the seed milk bud rate variation coefficient of the treated by the acidic water of the invention is small, that is, the germination rate is uniform, and the produced seeds are negatively consistent, and therefore, the germination treatment method of the present invention can be used. Launched sprouted seed products with the potential of the market to enhance the competitiveness of domestically produced agricultural products. [Embodiment] The present invention relates to a germination treatment method which is a method for inhibiting the growth of microorganisms by using acidic water to germination of seeds, and further provides a bactericidal effect growth by electrolyzed water having 1287434 which can effectively suppress microbial weak acidity. The acidic water utilized in the present invention can be added to an edible organic acid or other acidic substance such as acetic acid, 频 ® 彳, 彳 样 或 or glutamine to find the present invention. The desired acidic water. In the present invention, it is preferred that the system extracts an aqueous solution containing a low concentration of electrolyte from the reservoir to obtain acid water. In a preferred embodiment of the present invention, the decomposition of the aqueous solution of Gehuana via the electrode is carried out by using a low-concentration gasification nano-water bath 'Lee Xiao electrolysis principle, so that a trace amount of metal ions in the water changes, and a value of ρ 在 is generated at the anode. Low 'acidic water' for external disinfection.

Electrolyzed acidic water is currently known to have bactericidal efficacy. In addition to its low enthalpy value, its bactericidal function mainly includes effective gas components such as ^ (chlorine), H/CI (hypochlorous acid) and 〇cr (hypochlorite). Ion) (the only _ _ _ research sink, for '1 997) 'secondary acid is the main effective chlorine and the sterilization effect is the strongest. H〇c| is a volatile substance. After electrolysis, the gas reacts with water to form H〇CI, which gradually decreases with the pH value, so that the effective gas of ci2 is continuously reduced. Therefore, in an open system, the available chlorine will gradually disappear and disappear. According to the literature, electrolytic acidic water has the following characteristics: (1) Acidic water can be stable for 2 to 3 days at room temperature. (2) Instant bactericidal effect kills pathogenic bacteria. (3) It is made by electrolysis of salt and water, and the cost is low. (4) After many hospital tests, there will be no irritation or injury to the eyes, skin, mouth, esophagus, and mucous membranes. (5) After the action of acidic water, it will be reduced to ordinary water, which will not cause residue and pollution. 7 J287434 Dyeing environment. At present, the application of electrolytic acid water in the industry includes washing (chopper, knife, dish and towel) in the kitchen, restaurant, hand of the food processing factory, the environment or food cleaning... in the hospital. Prevention of internal infections and cleaning and sanitation of medical equipment. In agriculture, there is literature on the use of eggshells and decontamination or defecation of livestock manure. At present, the use in the aquaculture industry includes the cleaning, disinfection and deodorization of fish, and compares the electrolytic acid, water and tap water to the aquatic products of different water products such as squid and snails, respectively, and the electrolytic acid water is found. The bactericidal effect of the water product is slightly the same as that of the tap water; it is also found that the κ value of the fish treated with the electrolytic acid water is relatively slow and the storage period is extended. According to the technical data of the manufacturer of Japanese water ionizers (Ann〇nym〇us, 1 997), it is shown that electrolytic acidic water is used to treat malodorous substances such as trimethylamine (TMA) and methyl mercaptan. Its oxidation effect achieves the deodorizing effect. and

IzUmi (1 999) used 4G times of electrolytic acid water to clean vegetables such as radish, vegetables and potatoes, and found that it can effectively reduce the number of bacteria and E. coli = number two. Suzuki (1 996) used acidic water test. The test of various pathogens, the test results indicate that the acidic water has a bactericidal effect on the kitchen, and the main food poisoning bacteria - Streptomyces (Gaya (10)). Since the electrolytic acidic water has a bacteriostatic action, the present invention uses electrolytic acidic water for seed immersion, so that the electrolytic acidic water inhibits microorganisms during the immersion process; therefore, the number of bacteria in the immersion process can be solved too high during the germination process. The problem. 8 1287434 Specifically, the total amount of 电解 电解 〈 〈 〈 〈 〈 〈 Η佶 Η佶 Η佶 Η佶 Η佶 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解 电解Aminobutyric acid and vitamin E have no adverse effects, while having a lower number of bacteria and a higher germination rate. Therefore, the hair threading method provided by the present invention has the main features of: germination of seeds with electrolytic acidic water to produce germinated seeds by cold sputum, in order to produce a surgical effect and eliminate the initiation of water soaking. The smell, six does not have a good appearance and can

Provide better quality germinating seeds. The term "seed" as used in the present invention means a seed of various plants, and is not limited to edible or inedible seeds. Preferably, the edible seeds comprise dicotyledon seeds and single-night plant seeds, for example : Soy beans, mung beans, peanuts, brown rice. Since the seeds are germinated, the nutrients contained in the seeds are different from those before germination. According to research, the germinated seeds contain higher functional substances, such as r-aminobutyric acid (GABA). Therefore, the edible germinated seeds can be used. Further providing the nutrients of the ingestor different from the ungerminated seeds. It is known from the literature that when plant tissues encounter different stimuli, the pressure of these stimuli triggers a signalling pathway to activate Ca2+/carrying protein-dependent glutamate deferase (ca|m) by increasing cytosolic calcium content. Ind mountain independent glutamate decarboxylase) activity, promote GABA synthesis, such as contact and other agonistic factors induced by GABA accumulation, that is, the use of this way to increase the cytoplasmic Ca2+ content. In addition, increasing H+ and substrate content can also stimulate GAD (glutamate decarboxylase) activity. GAD is a cytoplasmic enzyme whose activity is highly positively correlated with germination rate. GABA accumulation is mainly regulated by GAD. The in vitro GAD of the most 9 1287434 is suitable for acidic pH of about 5 5, n κ, PH7.0 lack of ca2 + only _ 妓 activity. Regardless of intracellular and squamous stimulating GAD activity accumulation, in vitro = elephants suggest that lowering cytoplasmic sputum is more important than GAD accumulation:: cytoplasmic H + increase has been demonstrated pq & number of context factors stimulate GABA accumulation are indirect use Cytoplasmic pH is lowered ^, ...

— Life is low. Preferred acidic water pH in the present invention

The value of the dry circumference is PH3.0-5.0. P discloses the age of the present invention

— a specific embodiment to illustrate the method of the present invention and the effects achieved. All patents and publications cited in this specification are incorporated herein by reference. [Instrument Equipment] The equipment used in this embodiment includes: 1. Rotary incubator (Singapore incubator): S300R, Firstek, Taiwan. 2. Aseptic console (laminar flow): 2ht-24, comparable to the company, Taiwan. 3. Water bath: BC-2D, WISDOM, Taiwan. 4 · High-efficiency liquid chromatograph (Η PLC): Pump: L-6200, Hitachi, Co.Ltd,, Tokyo, Japan. Detector (UV-VIS detector): L-4200, Hitachi, Co.Ltd., Tokyo, Japan. Pipe column (〇〇丨11»11门): Ruler -18,6卩250-4.6(5/7〇1), Kanto Chemical Co., Ltd., Japan. 5·High speed freezing Centrifuge: All eg ra 64R Backman 1287434

Coulter, USA 6. Crusher: DM-6, Yusaki Machinery, Taiwan. 7. Ultrasonic cleaner: D-78224, El ma, Germany. 8·ρΗ analyzer (ph meter): SP-2200, Suntex, Taiwan. 9. Plant growth chamber: 747FH, WISDOM, Taiwan 1〇_Hand & type super acidified water generator: AL-700, Mettle r-Toledo, _ Switzerland The sewing rice used in the present invention is The Taichung Agricultural Improvement Field provides the No. 9 sugar rice in Taiwan, and the second rice in the Republic of China in 1993. Example 1: The germination method of germinated seam rice The method for preparing axillary bud brown rice is as follows: take the seam rice 1 〇〇 granules and place it in a 5 〇 ml k cup " slowly add A 3 沿着 along the wall of the cup Ultrasonic shock and degassing 20 knives of 4 tap water in the degassing place, the glutinous rice floating on the liquid surface is pressed into the liquid surface with a glass rod 'and gently (four)' so that the air attached to the surface of the sugar rice is discharged, and then placed A layer of foamed plastic film is placed on the liquid surface to insulate the outside air into the beaker. After the above treatment (4), it was transferred to a 95% dark plant growth box, and germinated for 24 hours to collect the germinated brown rice. Example 2: Effect of soaking water on germinated brown rice 2. Treatment of brown rice by different soaking water treatments = acidic water (9) ectrolyzed acidic wate "), using electrolysis raw materials to pass W tap water or adding low concentration gasification nano solution Then, through the decomposition of the electrode 11 1287434, the balance of the metal ions in the water changes, and the "acid water" which is low in pH value for external use is sterilized at the anode. The electrolytic acid water is currently known to have bactericidal efficacy, mainly including effective gas in the composition. Ingredients such as ci2 (air gas), H0C1 (hypochlorous acid) and 〇cr (hypochlorite ion), hypogastric acid is the main effective gas and has the strongest bactericidal effect. In order to understand the effect of soaking water on germinated brown rice, the inventor The brown rice was treated separately with tap water and electrolytic acid water, and the difference was tested. The preparation and preparation of a tap water soaked germinated brown rice and an electrolytic acid i soaked germinated brown rice were tested to compare the sewn rice, the tap water soaked fermented rice and the The difference in the number of bacteria, germination rate, gaba content and evaluation score of immersed glutinous rice in electrolytic acid water. Take 2_5 grams ( 1 〇〇 ) 的 台 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 The top of the beaker is covered with a layer of foam to isolate the air; then it is moved into a dark plant growth box. The sound and humidity are controlled at 25. 〇嶋%. After 24 hours of germination, the water is obtained. The electrolytic acid water soaks the germinated brown rice. 试验2 · 2 test results of different immersion water treatment of brown rice 2·2·1 bacteria number analysis After sewing rice, tap water soaked germinated seam rice and electrolytic acid water soaked germinated brown rice, Dilute the sterile water to the appropriate multiples and re-use pca culture (Merck, Germany) for 48 hours at 3 rc: calculate the number of colonies. Each treatment was repeated six times. The results are shown in the table. The number of bacteria obtained by soaking water to treat brown rice 12.1287434 Sample type Taiwan stalk 9th brown rice tap water soaked germinated brown rice electrolytic acid water soaked germinated brown rice bacteria number (106/g) ----- 2. 98±〇. 65a 7 · 45±〇· 25b 2· 92±〇· 6la The values listed in the table are the mean±standard deviation. The values marked with different English letters in the project are significantly different (P<0.05). From the data in Table 1, it can be seen that at temperature 25r, After tap water immersion = 24 hours, the number of bacteria was significantly increased by 2: times than that of ungerminated brown rice. After soaking for 24 hours with acidic water of pH 4.G, the number of bacteria was not: significantly lower than that of tap water. Germinated brown rice is even better than the fact that the number of bacteria in the germinated brown rice is lower, which can improve the problem that the gypsum process of the traditional germinated sugar rice production method may cause the g-growth to be too high and cause health concerns. 2 · 2 · 2 germination rate comparison In the present invention, the germination rate is calculated by the following formula: ^ bud rate (%) · (germination seam number / sampled brown rice number) χ彳〇〇 % # ^ brown rice, tap water soaked germinated brown rice And germination rate of soaked rice in electrolytic acid water. Each process is repeated three times. The results obtained are shown in the table yj\ 〇 * sample type

Germination rate κ germination rate (%) obtained by immersing germinated brown rice water in water treatment 51. 67±11. 841 (CV= 22.91%) 13 1287434 79. 〇±4· 32丨46%) Electrolyzed acidic water Soaked germinated sugar rice-----~----- The values listed in the table are the average standard deviation of the soil. The standard is different from the standard in English (p<0.05). The value of the parent is significantly different.广表2可! The data of acid water treatment _, / 2 also shows that the coefficient of variation (cv) of electrolytic acid water treatment is only 5.46%, and the coefficient of variation from the two = rate is 22.91%. It is advantageous to treat the resulting rice with the electrolytic acid water treatment and the production operation. Quantitative analysis of the tooth '2'2.3 Aminobutyric acid [r-Aminobutyric acid extraction] 1 gram of the split rice powder was placed in a 35 ml centrifuge tube, and then 1 liter of 75% ethanol was added and shaken. The centrifuge tube disperses the split rice powder at 7〇. (: After 1 hour of water bathing vibration i (15 rpm), centrifuge 2%, remove %, freeze and centrifuge for 20 minutes, collect the supernatant, and add 1 liter of 75% ethanol to the second water bath. After the above steps are repeated, the obtained supernatants are combined twice to obtain a preliminary extract. The extract is concentrated to dryness under a reduced pressure of 45 Torr, and 0.5 ml of 〇〇2N Hc丨 is added. Merck, Germany) solution, the concentrate is washed out and collected, centrifuged at 20 °, g xg, centrifuged at 4 ° C for 20 minutes, remove the supernatant, which is 7-aminobutyric acid extract, Store in a refrigerator and store with 7 _ aminobutyric acid. [T-Aminobutyric acid quantitation] 14 1287434 This example is a pre-column derivatization method using high performance liquid chromatography (HPLC) '7 -Aminobutyric acid was analyzed. Add 彳〇"L plant aminobutyric acid extract to 70 to derivatization reagent (AQC) special buffer (AccQ Tag WAT052880 'Waters) and 20/yL derivatization reagent (AccQ. Tag WA1T052880 'Waters), the total volume is 100//L, so that it is evenly mixed, let stand for 1 minute, so that it is not in the sample 7 _ The amino acid butyric acid-reactive AQc reagent is decomposed by itself and heated in a 50 ° C water bath for 1 , minutes to combine 7 • aminobutyric acid with a derivatizing reagent, cooled to room temperature, and filtered at 〇 2/Vm filter. ' Take 20/vl for HPLC analysis and determine the 7-aminobutyric acid content from the standard curve. Each treatment is repeated three times. High performance liquid chromatography conditions: Pump: L-6200 (Hitachi) Column ·· RP_18 , GP250-4.6 (5//m), Kanto Chemical Co., Ltd., Japan ° Detector · L-7485, Hitachi, Co. Ltd., Tokyo, Japan Fluorescence Detector >iex=295nm, Mem = 395nm Mobile Phase : A liquid mother solution contains 19.0g of sodium acetate-3-hydrate, 6.00g of ortho-phosphoric acid and 2_0g of triethylamine. Adjust pH to 5.15, quantify to 1 liter of pure water. B solution 60% acetoitrile / H2 〇 15 1287434 flow rate · · 1 ml / min Table 3 · r-aminobutyric acid HPLC analysis of mobile phase gradient mixing ratio

Soil 4·Gaba content obtained by treating brown rice with different soaking waters

Taiwan stalk 9 sugar rice tap water germination rough: rice electrolytic acid water soaked germinated brown rice 10·42±0.88a 10.98±1. 〇9a The values listed in the table are mean ± standard deviation * * The same item is marked with different English letters The values are significantly different (Ρ<0·05). As can be seen from Table 4, the accumulation of r-aminobutyric acid may be mainly regulated by glutamate decarboxylase, which is an enzyme in the cytoplasm, its activity and germination rate. It is highly positively correlated. Therefore, after germination of brown rice, the content of r-aminobutyric acid increased by about 2 VII', and the soaking of tap water and acidic water increased from 5·25 to 1 〇42 and 1 0.98 mg/1 〇〇g db, respectively (Table 4). There was no significant difference in the content of r-aminobutyric acid in the germinated brown rice after soaking with water. The experimental results showed that the pH of 16 1687434 electrolytic acid water had no effect on the content of germinated brown rice 7-aminobutyric acid and tap water soaking. difference. 2.2.4 Electrolytic acid water and tap water soaked germinated brown rice scent preference product evaluation This experiment uses 20 people to take the nine-point system for the sensory evaluation of odor preference, of which nine points represent the most favorite, five points do not like or hate One point represents the least favorite, and each reviewer must evaluate both samples. The scent of germinated brown rice (〇d〇r) was scored during the evaluation. The product evaluation of the germinated sugar rice sample was immersed in tap water and electrolytic acid water for 24 hours. The germinated brown water was gently blotted to the surface water without filter paper, and then about 20 grams was taken into a 50m glass beaker. The plastic wrap is covered and tied with a rubber band. During the evaluation, the reviewer is asked to loosen the wrap and evaluate the taste of the germinated brown rice. The evaluation is carried out at room temperature. The results of the evaluation are shown in Table 5. It is found that the preference score of the germinated brown rice after soaking with acidic water of pH 4〇 is 6 33 points higher than 4.97 points of tap water soaking. Therefore, the electrolytic acidic water should be prevented from germination: A bad odor is generated due to the growth of the bacteria or some bad metabolites are produced. Sample type, tap water, germinated, germinated brown rice, electrolytic acid water, soaked germinated brown rice, not treated with water, see the evaluation of the quality evaluation grade --------- 4. 92±〇· 76b 6· 33±1· 6〇a Column values are mean ± standard deviation 17

[Simple description of the schema] None, 1287434 There are significant differences in the values of different English letters in the same project (P<0.05) 2 · 3 Conclusions Eight comprehensives / The above results show that the immersion germination hair (four) meters of nutrition into private The Keding G text has been improved, and the acidity of pH 4 〇 is also used. ▲ t / and soaked water have a lower number of bacteria than the tap water soaking person: and the germination rate of the more uniform sentence 'also likes sexy The odor evaluation of the official evaluation is also higher than that of the self-supplied water. According to the present invention, the different modifications and variations of the bamboo can be made to the skilled person and the invention obviously does not deviate from the cost and spirit of the present invention. Although the present invention has described a particular preferred and defamatory affair, it must be unduly limited to such specific and enthusiasm. The local month should not be entitled to ★Taiwan like ▲, physical affairs only . Having described the invention as described above, the differences that are readily apparent to those skilled in the art are also covered by the scope of the τ lining. Μ正 [Main component symbol description]

Ml 18

Claims (1)

1287434, the scope of patent application · 1. A method for germination, comprising: providing seeds; and seed tweezers (4) in an aqueous solution having a pH of less than 7.5 to obtain germination: • as described in claim 1 The method further dries the hair seed to obtain dried germinating seeds. 3. The method of claim 1, wherein the aqueous solution having a ρ Η value of less than 7 _0 is produced by electrolysis containing an aqueous solution of electrolytic f ions. The method of claim 3, wherein the aqueous solution having a enthalpy of less than 7.0 is produced by electrolyzing an aqueous solution of sodium chloride. 5. The method of claim 1 or wherein the aqueous solution having a value of less than 7.0 is produced by adding an edible acidic substance. 6. The method of claim 5, wherein the aqueous solution having a enthalpy of less than 7.0 is produced by adding acetic acid, citric acid, anaphoric acid or a face acid. 7. The method of claim 1, wherein the time during which the seed is immersed in the aqueous solution having a pH of less than 7 〇 is from 2 hours to 36 hours 0 8 as in the patent claims 1 to 7. The method according to any one of the preceding claims, wherein the aqueous solution having a pH of less than 7.0 is pH 2 3 to pH 6 5 . 9 · The method described in claim 8 wherein the pH is 19 1287434’ An aqueous solution of less than 7.0 is pH 3.0 to pH 5.0. 1 0. The method of claim 8, wherein the seed is peanut, mung bean, soybean or brown rice. 1 1 The method of claim 1, wherein the seed is peanut, mung bean, soybean or rice. 1 2 - A germinated seed having a uniform germination rate obtained by the method according to any one of claims 1 to 11. XI. Schema: None 20