1251672 玖、發明說明： 【發明所屬之技術領域】 本發明係關於一種光電化學檢測方法及其裝置，特別 是一種應用於銅網版印刷電極領域、可增強檢測靈敏性與 便利性之流體光化學電流檢測方法及其裝置者。 【先前技術】 電化學檢測器是化學研究中新興的領域，其學術價值 及經濟效益都非常地可觀。檢測器的製造涉及許多學門之 專業知識’幾乎涵蓋了理、1、農、醫各領域，而電分析 化學更是當中的核心研究。-般檢測器需具備優良的選擇 性、高靈敏度及較短的偵測時間，若能兼具良好的稃定性 與可靠性，其主要發展空間，在於與流動注入系統（Flow Injection Analysis，FIA )、液相層析分離法（High Performance Liquid Chr〇mat〇graphy，Ηριχ )、或毛細管電 泳分離技術（Capillary Electr〇ph〇resis，CE)結合置 檢之靈敏度可實際應用於解決生醫與環保相關 ^ 問題。 个丁刀析 【發明内容】 由於目前傳統的電化學檢測器，在 並不佳，同時尚未有便利且完整的裝置來操作面 發明人經過努力研究與開發，以研發出—種結合光化^ 電化學之新穎光電化學檢測方法及可應用該方法之二、 以期增加液體檢測之準確性。 衣置， 本發明之主要目的係在於提供一種光電化學檢測 ”可以達到提高流體之檢測靈敏度之目的。 ' 1251672 本發明之另一主要目的係在於提供一種流體電化學檢 測瓜置’其可以實施上述之光電化學檢測方法，並能達到 簡易地替換檢測電極之目的。 為達到上述的目的，本發明之光電化學檢測方法主要 係為： 在一電化學檢測器中結合光電化學材質之電極，利用 照光的方式產生光電流以增強一檢測液體中的檢測電流， 以提南檢測之準確度。 為達到上述的另一目的，本發明之光電化學檢測裝置 其主要結構係包含有： 一基座，其具有一頂面、一前端與一後端，基座頂面 形成有一延伸至前端之容置槽； 一框設於基座後端之蓋體，該蓋體具有一頂面、一底 面、一 A端、一後端與兩側邊，蓋體底面形成有一具有内 開口，並對應容置槽之彈性分隔體，一光照孔與複數個流 孔形成於蓋體上並連通至分隔體之内開口範圍内； 放置於谷置槽中的檢測電極，該檢測電極對應光照 孔的位置上形成有具光電轉換性質之半導體材料；與 一設置於基座與蓋體之間之卡扣裝置。 其中卡扣裝置包含有： 兩個形成於基座頂面、含有定位珠之定位柱；與 一形成於蓋體前端上、對應定位珠之珠槽。 藉此，本發明利用照光的方式，配合光電化學材質之 電極，以增強檢測液體中的電流，以提高檢測之準確度； 1251672 置方面，利用盍體光照孔的設呈 質之半導俨i入⑴α ，…、男尤兒轉換性 、、“欢測電極的使用，以達到產生光化學電、、,拎、 樞設與卡扣川”/ J 基座與蓋體之間的 "，二 開啟蓋體以重新替換新的 核/、I極4片，讓檢測更為簡易便利者。 【實施方式】 本發明之光電化學檢測方法中’其主要為在—電化風 檢測：中結合光電化學材質之電極1用照光的方式產： 光電流以增強一檢測液體中的檢測電流以提高檢測之準 確度。 t發明中作為電極使用之光電化學材料可以為銅、銀 、金寻金屬之合金或衍生物’其中特別為銅合金方面例如 有銅鉑合金（CuPt)、銅鈀合金（CuPd)、銅汞合金（CuHg)、 銅銀合金(CuAg)、與銅金合金(CuAu)等等；其中特別為銅 的衍生物方面為氯化銅（CuC1)、溴化銅、碘化銅 (Cul)、氧化銅（cu〇)、氟化銅（CuF)、鈪化銅（CuAt)、或氧 化二銅（Cue)等等。其中Cu/O和Cun0為較佳之選擇，其 方法原理說明如下：BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a photoelectrochemical detection method and apparatus thereof, and more particularly to a fluid photochemistry that can be used in the field of copper screen printing electrodes to enhance detection sensitivity and convenience. Current detection method and its device. [Prior Art] Electrochemical detectors are an emerging field in chemical research, and their academic value and economic benefits are very impressive. The manufacture of detectors involves a lot of expertise in the field, which covers almost all areas of science, agriculture, and medicine, and electroanalytical chemistry is the core research. The general detector needs to have excellent selectivity, high sensitivity and short detection time. If it has good stability and reliability, its main development space lies in the flow injection analysis (FIA). The sensitivity of liquid chromatography separation (High Performance Liquid Chr〇mat〇graphy, Ηριχ) or capillary electrophoresis separation technology (Capillary Electr〇ph〇resis, CE) can be practically applied to solve the problems between biomedical and environmental protection. ^ Question. Analysis of the Ding Knife [Invention] Because the current traditional electrochemical detector is not good, and there is no convenient and complete device to operate the surface. The inventor has worked hard to research and develop to develop a kind of combined photochemical ^ Electrochemical novel photoelectrochemical detection method and the second method can be applied to increase the accuracy of liquid detection. The main purpose of the present invention is to provide a photoelectrochemical detection which can achieve the purpose of improving the detection sensitivity of a fluid. '1251672 Another main object of the present invention is to provide a fluid electrochemical detection melon which can implement the above The photoelectrochemical detection method can achieve the purpose of easily replacing the detection electrode. To achieve the above object, the photoelectrochemical detection method of the invention mainly comprises: combining an electrode of a photoelectrochemical material in an electrochemical detector, using illumination The method of generating a photocurrent to enhance the detection current in a detection liquid to improve the accuracy of the detection. To achieve the above other object, the main structure of the photoelectrochemical detection device of the present invention comprises: a susceptor Having a top surface, a front end and a rear end, the top surface of the base is formed with a receiving groove extending to the front end; a cover body disposed at the rear end of the base, the cover body having a top surface, a bottom surface and a bottom surface A rear end, a rear end and two side edges, the bottom surface of the cover body is formed with an elastic partition having an inner opening and corresponding to the receiving groove, and a light The hole and the plurality of orifices are formed on the cover body and communicate with the inner opening of the separator; the detecting electrode placed in the valley groove, and the semiconductor material having the photoelectric conversion property is formed at the position of the detecting electrode corresponding to the light hole And a fastening device disposed between the base and the cover body. The buckle device comprises: two positioning posts formed on the top surface of the base and including positioning beads; and a corresponding one formed on the front end of the cover body The bead groove of the bead is positioned. The invention adopts the method of illuminating, and cooperates with the electrode of the photoelectrochemical material to enhance the current in the detection liquid to improve the accuracy of the detection; 1251672, the use of the illumination hole of the corpuscle The semi-conducting 质i into (1)α,..., male and female conversion, "the use of the electrode to achieve photochemical power,,, 拎, pivot and buckle" / J pedestal and cover Between the two, the lid is opened to replace the new core/I pole 4 pieces, which makes the detection easier and more convenient. [Embodiment] In the photoelectrochemical detection method of the present invention, the main method is - electro-chemical Wind detection: The electrode 1 combined with the photoelectrochemical material is produced by illumination: the photocurrent is used to enhance the detection current in the detection liquid to improve the accuracy of the detection. The photoelectrochemical material used as the electrode in the invention may be copper, silver or gold. Metal alloys or derivatives, particularly copper alloys such as copper-platinum alloy (CuPt), copper-palladium alloy (CuPd), copper amalgam (CuHg), copper-silver alloy (CuAg), and copper-gold alloy (CuAu) Etc.; among them, in particular, copper derivatives are copper chloride (CuC1), copper bromide, copper iodide (Cul), copper oxide (cu〇), copper fluoride (CuF), copper telluride (CuAt). Or copper oxide (Cue), etc. Among them, Cu/O and Cun0 are the preferred choices, and the principle of the method is as follows:
Cu2 0和Cu 0都疋半導體材料（semiconcjuct〇r )並 具有光電化學的性質，如Cu/O是屬於具有多電洞（) 之P型半導體材料（semiconductor )，其電子躍遷的能 階差（Band gap )大約是1. 5- 0 ev，可吸收的光波長 範圍大約疋5 0 0 — 6 0 0 nm左右，因此常被用作為銅網版印 刷電極（CuSPE)之材料，讓CuSPE具有光電轉換現象。 1251672 基座（1 〇)為一四方形塊體，其具有一頂面、四個 側故、一 w端與一後端，基座（1 0 )頂面形成有一延伸 至引编之長條狀容置槽（1 2)，並於靠近後端處形成有 -1才區才幸 C Ί Ί Λ 1* 丄丄），此外，基座（1 0 )四個側邊上設有複 數迢止滑刻痕（1 〇 2 )，較佳地，該容置槽（丄2 )為 一定位效果較好的鳩尾槽。 疏版（2 〇 )係樞設於基座（1 〇 )上，該蓋體（2 〇)為一方形塊體，其具有一頂面、一底面、一前端、一 後鳊與兩側邊，蓋體（2 〇 )前端兩側各形成一肩槽（未 標號），蓋體（2 〇 )底面可形成有一具有内開口（未標 號）之彈性分隔體（未標號），複數個流孔形成於蓋體上 並連通至分隔體之内開口範圍内，較佳地，如圖中所示， 蓋體底面上形成有一環槽（28)，環槽（28)内容置 有一具作為分隔體之彈性方型墊圈（2 ，一光昭礼 (⑴嫌蓋體⑴）之頂面並連通i塾圈t‘28 2)圈住之範圍内，另有一第一流出孔（24)與一第二 流出孔（2 6 )分別形成與蓋體（2 〇 ) 一側邊，並有一 注入孔（2 7 )形成與蓋體（2 〇 ) $一側邊，同樣連通 至塾圈（282)戶斤圈住之範圍内。蓋體（2〇)後端形 成有-凹槽（2 1 ) ’該凹槽（2丄）可對應容置基座（ 1 0 )樞柱（1 1 )，並以一樞軸（丄^）穿設過蓋體 (2 0 )凹槽（2 1 )兩側與樞柱（丄丄），藉此將蓋體 (2 0 )與基座（1 Q )樞設連結。請進一步配合參考第 10 1251672 三圖’光照孔（22)中在靠近蓋體（2〇)广氏面處形成 有一限止以2 2 2 )，並有—透光破壤（2 2 4 )底靠 於限止環（2 2 2 )上，當蓋體（2〇) 了麼貼合基座（ 1 〇)時，塾圈⑴2)會在蓋體（2 〇)與基座（丄 0 )之間間隔出一檢測空間（a )。 卡扣裝置係設置於基座（10)與蓋體（20)之間 以將兩者緊密結合在_起，#包含有兩個形成於基座（工 〇 )頂面、含有定位珠（1 8 6 )之定位柱（i 4 )與一 形成於蓋冑（2 G )前端上、對應定位珠（工8 6 )之珠 槽（23)，當基座（10)與蓋體（2〇)蓋合時，每 一定位柱（1 4 )係對應於蓋體（2 〇 )每一肩槽之位置 ，而珠槽（2 3 )設於肩槽内，較佳地，定位柱（i 4 ) 内部形成有-螺孔（142)，並有—調節螺#(18) 栓設於螺孔（i 4 2 ) Θ ’調節螺桿（丄8 )軸向形成一 軸孔（1 8 2 ) ’並在軸孔（！ 8 2 )中容置設有一頂持 於定位珠（186)上的彈性體（184)，藉由彈性體 (1 8 4 )的彈力作用將定位珠（i 8 6 )壓迫推向珠槽 (2 3 )以提供卡掣的效果，其中調節螺拴（丄8 )可以 依鎖入螺孔（；L 4 2 )巾的深度來調節卡扣元件之緊度。 車又侄地，5亥彈性體（丄8 4 )為—彈簧。當蓋冑（2 〇 ) 貼合座體（1 〇 )時，定位珠（i 8 6 )會卡f於珠槽（ 23)中，以固定住蓋體（2〇)於座體（1〇)上讓 墊圈（2 8 2 )緊壓於基座（！ 〇 )上形成檢測空間（a )’當開啟蓋體（2 〇 )時’只須施力讓定位王朱（丄8 6 1251672 )推動彈性體（8 4 )以脫離珠槽（2 3 )即可，因此 ，對於開啟或關閉蓋體（2 0 )都相當簡便。 檢測電極（5 〇 )係為置於容置槽（丄2 )内，其長 度略大於容置槽（1 2 )，朝上面具有一對應環槽（2 8 )X置的内接點（5 2 )，並設有一連接一讀取裝置的外 接點（5 4 )，該内接點（5 2 )上須要形成一層具有光 電轉換性質之半導體材料，如氧化亞銅等。 ^ 此外，請參考第一圖與第四圖，本發明之光電化學檢 測裝置’須要在蓋體（2 〇 )關閉之後，自注入孔（2 7 )注入流體並從第一或第二流出孔（2 4 ) ( 2 8 )導出 流體，當檢測時在檢測電極上接通一適當電壓，並以一發 光源（5 5 )穿入照光孔（2 2 )中，透過照光孔（2 ; )與透光玻璃（2 2 4 )，讓光能進入檢測空間（a )， 激發㈣f極（5 〇 )内接( 5 2 )上的半導體材料產 生光化學電流’以放大電流檢測之反應，增 靈敏度。 此外，本發明之光電化學檢測裝置可依需要配合使用 參考電極或輔助電極連接於第一流出孔（2 4 )或第一、七 出孔（2 6 )使用，以增加檢測器之操作變化性。 為二證明本發明中光電化學檢測方法之效果以下為 -測試貫驗：利用上述之自製光電化學檢測震置，進"對 於苯鄰二紛類化合物（0HPs)的债測，首先針 ：光 源強度對整個實驗的影響做評估。 請參照第五圖所示，為以安培伏安法在fu系統中配 12 1251672 合150W鹵素燈（Halogen lamp)照射以偵測OHPs的結 果°觔300秒未施加光源（Light off)，注入100 μΜ CA 時的電流訊號為〇. 33 μΑ。300秒之後開始將光源依次以 不同的強度打開（on — a，-b，-c和max· -d )，並在不同光 源強度之下，也分別注入1〇〇 CA。由圖中發現，每當 光源更強日才其背景光電流（Background photocurrent )也 就更大’這也更證明了中所推測的光電化學反應的機構， 亦即背景光電流是由價帶（VB )的電子被激發後，流向電 極方向的光電化學電流（/pEc )所致。同時，隨著背景光 _ 電流地增加，所注入的CA電流訊號也跟著增加放大，由 第六圖的線性回歸線（B圖）上，可以更清楚看出其改變 趨勢’以實驗室目前的150 W鹵素燈（Halogen lamp)， 可以將CA的偵測電流最大增加至267 %，而且增大的電流 也尚未飽和。相信若使用更大功率的光源，一定可以再增 加偵測的靈敏度。此外，本發明已經過美國分析化學期刊 的認證其正確性，並獲發表於2 0 0 3年1 2月1 5曰美 國分析化學期刊中（内容詳見附件）。 春 綜上所述，本發明由於藉由光照與具有光電轉換性質 之半導體檢測電極（5 〇 )的使用，以達到產生光化學電 流增加檢測裝置的靈敏度的優點，另一方面，裝置中基座 與蓋體之間的樞設與卡扣裝置的設置’彳以輕易的開啟或 結合蓋體，以重新替換基座上之檢測電極試片，因此具有 操作簡易便利之的特性，以簡化檢驗操作，具有相當的進 步性、實用性及產業利用性，符合發明專利巾請要件 13 1251672 此具文提出發明專利申請 【圖式簡單說明】 月 (一）圖式部分 第一圖 第二圖 弟三圖 别視圖 β係為本發明之立體分解圖。 :係為本發明呈閉闔狀態之上面剖視圖。 :係為本發明沿第二圖中3 一 3線之前側 第四圖 狀態側面剖視圖 係為本發明沿第二圖中4 一 4線之操作 光日I二圖··係為仙GHPs操作實驗結果之時間 圖（A二"人電流以對應所制電流強度之對照❹ 圖（A)與長條圖（B)。 光的^第六圖：係為偵測0HPs操作實驗中照光或不用 九的it形下，時間盥 對照曲線 ;、輪…大小對應所價測電流強度3 口〈 a )與線性回歸圖（B )。 (二）元件代表符號Both Cu2 0 and Cu 0 are semiconductor materials (semiconcjuct〇r ) and have photoelectrochemical properties. For example, Cu/O is a P-type semiconductor material with multiple holes (Semiconductor), and its energy transition energy level difference ( Band gap ) is about 1.5- 0 ev, and the absorbable light wavelength range is about 疋500-600 nm. Therefore, it is often used as a material for copper screen printing electrodes (CuSPE) to make CuSPE have photoelectric Conversion phenomenon. 1251672 The base (1 〇) is a square block having a top surface, four sides, a w end and a rear end, and the top surface of the base (10) is formed with a strip extending to the lead strip. The groove (1 2) is accommodated, and the -1 area is formed near the rear end. C Ί Λ Λ 1* 丄丄), and the pedestal (10) has a plurality of sides on the four sides. Preferably, the accommodating groove (丄2) is a tail groove having a better positioning effect. The stenciling (2 〇) is pivoted on the base (1 〇), the cover body (2 〇) is a square block having a top surface, a bottom surface, a front end, a rear sill and two sides A shoulder groove (not labeled) is formed on each of the two sides of the front end of the cover body (2 〇), and the bottom surface of the cover body (2 〇) can be formed with an elastic partition (not labeled) having an inner opening (not labeled), and a plurality of flow holes Formed on the cover body and connected to the inner opening of the partition body, preferably, as shown in the figure, a ring groove (28) is formed on the bottom surface of the cover body, and the ring groove (28) is provided with a partition as a separator. The elastic square washer (2, the top surface of the light curtain (1) the cover body (1)) and the i塾 circle t'28 2) enclosed within the range, and a first outflow hole (24) and a second The outflow holes (26) are respectively formed on one side of the cover body (2 〇), and an injection hole (27) is formed on one side of the cover body (2 〇), and is also connected to the 塾 ring (282) Within the scope of the circle. The rear end of the cover body (2〇) is formed with a groove (2 1 ). The groove (2丄) can accommodate the base (10) pivot post (1 1 ) and is pivoted (丄^ The two sides of the recess (2 1 ) of the cover body (2 0 ) are pierced with the pivot post (丄丄), thereby pivotally connecting the cover body (20) to the base (1Q). Please further refer to the reference to the 10th 1216672 three figure 'light hole (22) in the vicinity of the cover (2 〇) Guangshi surface formed a limit to 2 2 2), and there - light broken soil (2 2 4) Depending on the limit ring (2 2 2 ), when the cover (2〇) fits the base (1 〇), the ring (1) 2) will be in the cover (2 〇) and the base (丄0) A detection space (a) is spaced apart. The fastening device is disposed between the base (10) and the cover body (20) to tightly bond the two together, and #includes two top surfaces formed on the base (worker), and includes positioning beads (1) 8 6 ) The positioning post (i 4 ) and a bead groove (23) formed on the front end of the cover (2 G ) corresponding to the positioning bead (work 8 6 ), when the base (10) and the cover body (2〇) When the cover is closed, each positioning post (14) corresponds to the position of each shoulder groove of the cover body (2 ,), and the bead groove (23) is disposed in the shoulder groove, preferably, the positioning post (i 4) The inside is formed with a screw hole (142), and the adjusting screw #(18) is bolted to the screw hole (i 4 2 ) Θ 'The adjusting screw (丄8) axially forms a shaft hole (1 8 2 )' And an elastic body (184) supported on the positioning bead (186) is received in the shaft hole (! 8 2 ), and the positioning bead (i 8 6 ) is acted upon by the elastic force of the elastic body (1 8 4 ) The pressing is pushed toward the bead groove (23) to provide the effect of the click, wherein the adjusting screw (丄8) can adjust the tightness of the snap element depending on the depth of the locking screw hole (; L 4 2 ). The car is slammed, and the 5 liter elastomer (丄 8 4 ) is a spring. When the cover (2 〇) is attached to the seat (1 〇), the positioning bead (i 8 6 ) will be snapped into the bead (23) to secure the cover (2〇) to the seat (1〇) ) Let the washer (2 8 2 ) press against the base (! 〇) to form the detection space (a ) 'When opening the cover (2 〇), 'only need to apply force to position Wang Zhu (丄 8 6 1251672 ) Pushing the elastomer (8 4 ) away from the bead groove (23) is therefore quite simple for opening or closing the cover (20). The detecting electrode (5 〇) is placed in the accommodating groove (丄2), and its length is slightly larger than the accommodating groove (1 2 ), and has an inner contact point corresponding to the ring groove (2 8 ) X on the upper side (5) 2), and is provided with an external contact (5 4 ) connected to a reading device, and a semiconductor material having photoelectric conversion properties, such as cuprous oxide, is formed on the inner contact (52). ^ In addition, referring to the first and fourth figures, the photoelectrochemical detecting device of the present invention needs to inject fluid from the injection hole (27) and from the first or second outflow hole after the cover (2 〇) is closed. (2 4 ) ( 2 8 ) deriving the fluid, when detecting, turning on an appropriate voltage on the detecting electrode, and passing through a light source (5 5 ) into the illumination hole (2 2 ), passing through the illumination hole (2; ) With the light-transmissive glass (2 2 4 ), let the light enter the detection space (a), and excite the (four) f-pole (5 〇) to inject the photochemical current of the semiconductor material on the (5 2 ) to amplify the reaction of the current detection. Sensitivity. In addition, the photoelectrochemical detecting device of the present invention can be used by using a reference electrode or an auxiliary electrode connected to the first outflow hole (2 4 ) or the first and seventh outlet holes (26) to increase the operational variability of the detector. . In order to prove the effect of the photoelectrochemical detection method in the present invention, the following is: test pass: using the above-mentioned self-made photoelectrochemical detection to detect the shock, and to measure the bond of the phthalate compound (0HPs), firstly, the needle: the light source The intensity is evaluated for the impact of the entire experiment. Please refer to the fifth figure for the results of Detecting OHPs by ampere voltammetry in a fu system with 12 1251672 and 150W Halogen lamp. The ribs are not applied to the light for 300 seconds. The current signal at μΜ CA is 〇. 33 μΑ. After 300 seconds, the light source is turned on in turn with different intensities (on — a, —b, -c, and max· -d ), and 1 〇〇 CA is also injected under different light source intensities. It is found in the figure that the background photocurrent is larger whenever the light source is stronger. This further proves the mechanism of the photoelectrochemical reaction presumed in the photo, that is, the background photocurrent is determined by the valence band ( After the electrons of VB) are excited, the photoelectrochemical current (/pEc) flowing in the direction of the electrode is caused. At the same time, as the background light _ current increases, the injected CA current signal is also increased and amplified. From the linear regression line (B) of the sixth graph, the trend of change can be more clearly seen. The Halogen lamp can increase the detection current of CA up to 267%, and the increased current is not saturated. It is believed that if a more powerful light source is used, the sensitivity of the detection can be increased. In addition, the present invention has been certified by the American Journal of Analytical Chemistry for correctness and was published in the January 2015 issue of the American Journal of Analytical Chemistry (see attachment for details). As described above, the present invention achieves the advantage of generating a photochemical current increase detecting device by the use of illumination and a semiconductor detecting electrode (5 〇) having photoelectric conversion properties, and on the other hand, the pedestal in the device The pivoting connection with the cover body and the setting of the snap device 彳 to easily open or bond the cover body to replace the test electrode test piece on the base, so that the operation is simple and convenient, so as to simplify the inspection operation. , with considerable progress, practicality and industrial utilization, in line with the invention patent towel requirements 13 1251672 This article proposes invention patent application [simple description of the picture] month (1) pattern part first picture second picture third three The figure view is a perspective exploded view of the present invention. : is a top cross-sectional view of the present invention in a closed state. The present invention is a side view of the fourth side of the third line in the second figure. The side view is the operation of the invention along the 4th and 4th lines in the second figure. The time chart of the results (A two " human current to control the current intensity of the contrast diagram (A) and bar graph (B). The sixth figure of the light: is to detect the 0HPs operation in the experiment or not In the case of nine, the time 盥 comparison curve; the size of the wheel corresponds to the measured current intensity of 3 mouth < a ) and the linear regression chart (B ). (2) Component symbol
〇 )基座 1 )樞柱 (1 0 2 )止滑刻痕〇) pedestal 1) pivot post (1 0 2) anti-slip scotch
1 1 )拖轴 4 )定位柱 8 )調節螺桿 8 4 )彈性體 0 )蓋體 2 2 )限止環 (12)容置槽 (1 4 2 )螺孔 (1 8 2 )軸孔 (1 8 6 )定位珠 (2 2 )光照孔 (2 2 4 )透光玻璃 14 1251672 (2 3 )珠槽 (2 4 )第一流出孔 (2 7 )注入孔 (2 8 )環槽 (3 0 )參考電極 (3 4 )第二金屬桿 (4 0 )輔助電極 (4 4 )第一金屬桿 (5 0 )檢測電極 (5 2 )内接點 (2 6 )第二流出孔 (2 8 2 )〇型環 (3 2 )螺桿 (4 2 )螺桿 (5 0 a )檢測電極1 1 ) Towing shaft 4 ) Positioning post 8 ) Adjusting screw 8 4 ) Elastomer 0 ) Cover 2 2 ) Limiting ring (12) accommodating groove (1 4 2 ) Screw hole (1 8 2 ) Shaft hole (1 8 6) positioning bead (2 2 ) illumination hole (2 2 4 ) light transmissive glass 14 1251672 (2 3 ) bead groove (2 4 ) first outflow hole (2 7 ) injection hole (28) ring groove (3 0) Reference electrode (3 4 ) second metal rod (40) auxiliary electrode (4 4 ) first metal rod (50) detection electrode (52) inner contact (2 6) second outflow hole (2 8 2 ) 〇-ring (3 2 ) screw (4 2 ) screw (5 0 a ) detection electrode
(5 4 )外接點 (a )檢測空間 (三）附件 本發明於2 0 0 3年1 2月1 5日美國分析化學期刊 第75冊第24卷，第7020—7025頁所發表之公 開本。（網路公開曰期為2 0 0 3年1 1月1 5曰）(5 4) External point (a) Detection space (3) Attachment The present invention was published in the American Journal of Analytical Chemistry, Vol. 75, Vol. 24, pp. 7020-7025, January 15, 2003. . (The network is open for the public season of 203, 1 January, 1 5 曰)
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