1238889 _ 五、發明說明（1) <發明所屬之技術領域> 本發明係提供一種微流道晶片之微量流體黏性量測方 法及其裝置，尤指其流體得於微流道内依離心力漸變增加 至 疋值時得開始流動，流動經啟始量測點及量測終點之 距離與時間得為雷射感測器與計時器檢測記錄，配合轉速 之相關數值在經由查表或代入公式後，乃得以測出流體之 黏性’據此達其流體黏性量測功效者。 <先前技術> 研究生物晶片或微流體系統得先建立一微流體驅動平 台方可，一般的微流體驅動平台係「固定式」，亦即在一 固定平台上，研製組合出一個小型的流體系統，内含微泵 j pump )、微閥、微流通道及微流感測器等元件，但這些 微組件的開發與組合，不是動辄耗費昂貴，就是微小接頭 、微泵（pump )的不易處理，因微流道的阻力極大而常常 出現沒漏的問題，使此等研究仍停留在構想與實驗階段。 、為了解決上述困境，便有人率先提出以轉盤的離心力 取代微泵（Pump )來驅動微流體的系統，並藉由純幾何形 ，的所謂毛細管開關（Capillary switch )取代複雜的 微閥，其實驗證明，只要改變轉盤的轉速，在不同半徑上 :儲槽試液，會依續外流到一主槽進行反應，遂行單一 「晶片」可順序反應、篩檢的目的。由於此轉盤型驅 莫ϋ與其他方式比較起來’有相對簡單、價廉、無浪漏 專優勢，故很值得加以研究、改進與推廣。 本發明之發明人從事微流道晶片測試研究不遺餘力，1238889 _ V. Description of the invention (1) < Technical field to which the invention belongs > The present invention provides a microfluidic chip microfluidic viscosity measurement method and its device, especially the fluid obtained from the microfluidic channel according to centrifugal force When the gradual increase to the threshold value, it can start to flow. The distance and time of the flow through the initial measurement point and the measurement end point can be recorded by the laser sensor and the timer. Later, it was possible to measure the viscosity of the fluid ', which is the basis for measuring the viscosity of the fluid. < Previous technology > A microfluidic driving platform must be established before researching biochips or microfluidic systems. The general microfluidic driving platform is "fixed", that is, a small platform is developed and assembled on a fixed platform. The fluid system contains micropumps, microvalves, microfluidic channels, and microfluidic detectors. However, the development and combination of these microcomponents are either expensive and expensive, or they are micro joints and pumps. It is not easy to handle, and the problem of leaks often occurs due to the great resistance of the microchannels, so that these studies are still at the stage of conception and experiment. In order to solve the above-mentioned dilemma, someone first proposed a system that uses a centrifugal force of a turntable to replace a pump to drive a microfluidic fluid, and replaced the complicated microvalve with a pure geometrical so-called capillary switch. It is proved that as long as the rotating speed of the turntable is changed, the test fluid in the storage tank will continuously flow out to a main tank for reaction in different radii, and the single "chip" can be used for sequential reaction and screening purposes. Compared with other methods, this turntable type drive is relatively simple, cheap, and has no leaks, so it is worthy of research, improvement and promotion. The inventor of the present invention spared no effort in the research of microchannel wafer testing,
第4頁 1238889 五、發明說明（2) 針對先前「離心式微流體驅動觀測系統」研發呈一 —、、a ^乃對其相關之應用作一系列之研究，心試驗：：： 為實用，Μ由多年之努力研究並屢為試作，終於創作出、 ^明之「微流道晶片之微量流體黏性量測方法及其裝置」 <發明内容> 本發明之主要目的，即在於強化微流道晶片試 ，而提供一種微流道晶片之黏性量測方法及其裝置， 該轉盤同軸得設一固定盤，固定盤上固定二雷射感^ =二得感測轉盤上微流道晶片之量測起點與量測 二：：固定有雷射計時器’據以轉盤在漸變之 ”” t ’乃得以記錄轉速及其試驗流體初始流動= 關係，k體經過量測起點與量測終點之時間則由計 =之霉mi ^查表或代入公式計算後，得該流 ’達到微量流體黏性量測方法及裝置之可行與實 用’〖功效，且其相關設備費用亦較簡省，為其特徵者。 佳管ϊ，ΐ發明所採用之技術、手段及其功效，兹舉一較 配合圖式詳細說明於后，相信本發明上述之目 :實=特徵，當可由之得一深入而具體。 <貫施方式> 法及提供一種徵流道县片之撤*流*黏性… 配合瞭解本發明之結構設計及技術，謹Page 4 1238889 V. Description of the invention (2) A series of researches on the research and development of the previous "centrifugal microfluidic drive observation system" were performed on the related applications. Heart test: For practical use, M After years of hard research and repeated trials, he finally created, "A microfluidic chip microfluidic viscosity measurement method and device" < Content of the invention > The main purpose of the present invention is to strengthen microfluidics. The invention provides a method for measuring the viscosity of a microchannel wafer and a device thereof. The turntable is coaxially provided with a fixed plate, and two laser sensors are fixed on the fixed plate. Measurement start point and measurement two: The laser timer is fixed, according to which the turntable is gradually changed "" t "is to record the rotation speed and the initial flow of the test fluid = relationship, the body k passes through the measurement start point and the measurement end point The time is calculated by looking at the table or substituting the formula, and the flow is 'reachable and practical for the measurement method and device of trace fluid viscosity' [effect, and its related equipment costs are relatively simple and cost-effective. CharacteristicThe technology, means and effects used in the invention are well explained. I will explain it in detail with reference to the drawings. It is believed that the above purpose of the present invention: real = feature, which can be obtained in-depth and specific. < Performance method > Method and providing a method of retreating * flowing * adhesiveness of the levied county film ... With the understanding of the structural design and technology of the present invention,
1238889 ^----— 五、發明說明（4) 電腦主機（4 Ο )記錄並儲存 電腦主機（40) 相關資料與影像資料， 、流體流動起i ί 到試驗並記錄轉盤（1〇)轉速 功效者。時間點、流體流速，據以換算出流體黏性 發明特點： ^ ^離心式微流道晶片測試機具配合黏度試片，達 到僅以被置流體即可暑、、目丨、古胁 供Ai 4 流體黏性之可行性，並使整體設 備成本最簡省功效者。 二、黏度試片得依所需製作相關之微流道，達其配人 相關之黏度試驗者。 " 為 微流道晶片之黏性量測方法及其裝置，操作步驟程序 將合適大小、長度之黏度量測微流道試 附固定於轉盤觀測區上。 月」貼 2 4 調整女:起點與終點之微點雷射感測器，用精密針筒量 取定量之待測流體注入於試片之試樣起始儲槽中。 打2:2雷射計時電路電源’調整、操作離心式 微二m:測裝[使轉盤逐漸加速旋轉，由擷取 2 "中觀察待測流體是否因離心力作用而自 槽甲机’並通過兩個晋：丨 Μ 影彳t，Μ ^ 後，流入終點儲槽中。 利始m由絲i笛射°十時器$到之通過時間記錄， 關掉電源使轉盤停止；依所 ^ ^ ^ Λ X 依所圯錄之轉速與流體通過時 間查表或代入公式針11u 式4异即可得其黏度值。1238889 ^ ----— V. Description of the invention (4) The main computer (4 Ο) records and stores the relevant data and image data of the main computer (40), the fluid flows from i to the test and records the speed of the turntable (10) Efficacy. The time point and fluid flow rate are used to calculate the viscosity of the fluid. Features of the invention: ^ ^ Centrifugal microfluidic wafer testing equipment with viscosity test strips, which can be used to supply Ai 4 fluid only with the placed fluid. The feasibility of stickiness and minimizes the overall equipment cost. 2. Viscosity test strips can be made with relevant micro-channels as required to reach the relevant viscosity tester. " It is a method and a device for measuring the viscosity of a micro-channel chip, and the operation procedure is to attach a micro-channel of a suitable size and length to the micro-channel to be fixed on the observation area of the turntable. "Month" stickers 2 4 Adjusting female: Micro-point laser sensors at the starting point and the ending point, use a precision syringe to measure the amount of fluid to be measured and inject it into the sample starting storage tank of the test piece. Hit the 2: 2 laser timing circuit power supply to adjust and operate the centrifugal microsecond m: test equipment [make the turntable gradually accelerate rotation, observe in the capture 2 " whether the fluid to be measured from the tank A machine due to centrifugal force 'and pass Two Jins: After M t, M ^, flows into the terminal storage tank. Leshim records the passing time of the 10 o'clock timer when the flute is fired, turn off the power to stop the turntable; check the table according to the recorded speed and fluid passage time or substitute the formula needle 11u Equation 4 can be different to get its viscosity value.
麵 1238889 .—-—— 玉、發明說明（5) » 5 ·取下黏度f測微流道試片，同時可準備 之 操作。 下一次量踯 綜上所述，本發明在同類產品令實有其極 、 用性’同時遍一國内外關於此類結構之技術資料、文獻中 亦未發現有相同的構造存在在先，是以，本發明實已具備 發明專利要件，爰依法提出申請。 上述實施例，僅用以舉例說明本發明，據以在不離本 ，明精神之範圍，熟習此項技藝者憑之而作之各種變形、 仏飾與應用，均應包括於本發明之範疇者。Surface 1238889. ————— Jade, description of invention (5) »5 · Remove the test piece of viscosity f micrometer flow channel, and it can be prepared at the same time. To sum up, the next time, according to the present invention, similar products make them extremely useful and useful at the same time. At the same time, domestic and foreign technical data on such structures have not been found in the literature. Therefore, the present invention already has the elements of an invention patent, and an application is filed in accordance with the law. The above-mentioned embodiments are only used to illustrate the present invention. According to the scope of the present invention, the various modifications, decorations and applications made by those skilled in the art should be included in the scope of the present invention without departing from the spirit. .
1238889 圖式簡單說明 <圖式之簡單說明> 第1圖：係本發明之微流道晶片測試裝置之立體示意圖。 第2圖：係本發明之黏性量測裝置立體圖。 第3圖：係微流體試驗機其一較佳實施例圖。 <圖式之圖號說明> (10)轉盤 （11)可變速馬達 (12)電子電路 （13)調速器 (2 0 ) C C D攝影機 (31)微流道 (3 3 )終點儲槽 (4 1 )電腦顯示器 (5 3 )起點 (5 5 )雷射計時器 (1 4 )轉速顯示器 (3 0 )微流道晶片 (32)起始儲槽 (4 0 )電腦主機 (5 0 )起點微點雷射感測器 (5 1 )終點微點雷射感測器 (5 2 )固定盤 (5 4 )終點1238889 Brief description of the drawings < Simplified description of the drawings > Figure 1: This is a schematic perspective view of the micro-channel wafer test device of the present invention. FIG. 2 is a perspective view of the viscosity measuring device of the present invention. Fig. 3: A diagram of a preferred embodiment of a microfluidic testing machine. < Illustration of drawing number of the drawing > (10) Turntable (11) Variable-speed motor (12) Electronic circuit (13) Governor (2 0) CCD camera (31) Micro-fluid channel (3 3) End-point storage tank (4 1) Computer display (5 3) Starting point (5 5) Laser timer (1 4) Speed display (3 0) Micro channel chip (32) Starting storage tank (4 0) Computer host (50) Start point micro-point laser sensor (5 1) End point Micro-point laser sensor (5 2) Fixed plate (5 4) End point