1231179 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種漂浮性微生物作用劑之製造方法及其 於生物防治之應用，以防治生物受病害感染之機會。 【先前技術】 、一般在對於防治植物病蟲害，以往皆以使用農藥來進行防 治病蟲害，以提高作物產量及降低作物罹患病害的機率。 ，農藥本妓—種化學製劑，若的濃度過高及接近採收後 用，此因使用的方法不當，會使作物產生農藥殘留的毒 =題^而危及人們的健康，造成農藥積聚於人們體内或急性 甲毋現象。 而現階段在轉農藥方式防治植物病害上， 以一種微生物 ΐ進==生=台’已成為可能，利用微生物來防治植‘ 微生防治方法時’是利用喷麗方式將該防治性 侵入的位置最常見於葉鞘上，若二例，病原 的過程，會因為實施噴灑的水苹择===防^蟲害 於植物體上產生傷口，同時因嘖^:二，，動，造成水稻 口處見於水雖物體上，非但不ί有“二荦=成^傷 巧他病原菌侵人該受傷水稻的機會，使發 究並 劑之製造村及其於生_之顧』' 1231179 失與傷害。以下為本案之簡要說明。 【發明内容】 伽ίϊ明，目的在於提供—種漂浮性微生物作用劑以代替 傳、、先^樂之使用，解決大量農藥被濫用與使用不當之 μ从發明之另—目的在於提供—種漂浮性作用劑及盆庫用 式，能正確的進入病原造成植物病二途2: 置，以提幵病害防治與殺死病原的效果。 本發明之又—目的域供—種漂雜作㈣及其 r Γίί該漂浮性作用劑作用於該受作用^植物 卤之活性，達到防治病害之目的。 闲原 是^本個之主要目的’在提供—種漂雜作用劑 ϊί ni法包含提供—種拮抗微生物，該拮抗微生物^作 用二，體，使該拮抗微生物與—黏合物、 及加入—凝聚劑，俾予凝聚該中間^ ㈣其中該漂浮性作用劑更包含-載體，用以 ? Gliocladium Virens ^ (Rhizoctoniasolani) AG-1 〇 根據上述構想，置中讀激人I & # ^ 糾、，AM人/、τ Θ黏合物為藻酸鈉（Sodium Algmate) ’供以作黏合之用；該培 =養該=微生=爾射叹 ，疋工乳’以提供5亥/示〉于性作用劑具有一漂浮性質， 不 與水親和之物質為-麵錄沙㈣之大豆沙拉、、^、“ i納ΪΓ貝Αί之?f合:產生凝聚作用，其中該黏合物為蒸 酉夂鈉(SodiumAlgmate)，為供以作黏合之用。 1231179 於攜用劑，適用 一凝聚作用，以生成該㈣，與該黏合物產生 法適錄病害之方 該漂浮性作用劑且有^士^ / ^ 3S供—漂洋性作用劑， 過該水耕植物==防治—植物病害，透 式嶋觸之方 Μ 明之再另一重要目的為提供一種生物防户之導入▲ 微生物而運送二植^^性作用劑，係用以攜帶該拮抗 浮性作用丄 ί ’ 抗微生物，—漂浮劑，具有-漂ΐ性質Ί 及一料劑，與該黏合物產生一凝聚作用。 w貝以 得-解本發明得藉由下面之實施例中之詳細說明，俾 【實施方式】 ^，明提供一種漂浮性作用劑之最佳製造方法， 其方法 ;ΞΞΗΞΞ3 劑 述 1231179 1提供以-載體，用以攜帶該拮抗微生斗勿。在1231179 IX. Description of the invention: [Technical field to which the invention belongs] The present invention relates to a method for manufacturing a floating microbial agent and its application to biological control, in order to prevent the opportunity of biological infection by disease. [Previous technology] In the past, generally, for the prevention and control of plant diseases and insect pests, pesticides were used to control the diseases and insect pests in order to increase crop yield and reduce the probability of crop diseases. The pesticide is a kind of chemical preparation. If the concentration is too high and it is used close to the harvest, this method will cause the pesticide residue poison to the crop due to improper use of the method. It will endanger people's health and cause pesticides to accumulate in people. In vivo or acute hypothyroidism. At the current stage, in the conversion of pesticides to control plant diseases, it has become possible to use a microorganism to invade == raw = plants. It is possible to use microorganisms to control plants. The location is most common on the leaf sheath. In the second case, the pathogenic process will be caused by the spraying of water spray === to prevent insects from causing wounds on the plant body, and at the same time, it will cause rice mouths to move. Seen on water objects, not only does it not have the opportunity of "II 荦 = 成 ^ wounding his pathogenic bacteria to invade the injured rice, so that the research village and its __ Gu's" 1231179 loss and injury. The following is a brief description of this case. [Summary of the Invention] The purpose of the invention is to provide a floating microbial agent instead of the use of ruminant and selenium to solve the abuse and improper use of a large number of pesticides. —The purpose is to provide—a floating agent and pot-and-pot type, which can correctly enter the pathogen and cause plant diseases in the second way 2: in order to improve the effect of disease control and kill the pathogen. —Species drift Miscellaneous crops and its r Γίί The floating agent acts on the affected ^ plant halogen activity to achieve the purpose of disease prevention and treatment. The main purpose of this source is to provide a kind of floating agent ϊNi method The method includes providing an antagonistic microorganism, the antagonistic microorganism acting as a body, enabling the antagonistic microorganism and -adhesive, and adding -aggregating agent, to agglomerate the intermediate agent, wherein the floating agent further comprises a carrier, and Gliocladium Virens ^ (Rhizoctoniasolani) AG-1 〇 According to the above-mentioned concept, the reading reader I &# ^, AM, / τ Θ is sodium alginate (Sodium Algmate) for the adhesion The purpose of this cultivation is to raise the nutrition = micro life = Seoul She sighs, the maternal milk 'to provide 5 Hai / show> the sex agent has a floating nature, the substance that is not compatible with water is-soy sauce Soybean Salad, ^, "i NaΪΓ 贝 Αί 之? F combination: produces a coacervation effect, where the adhesive is Sodium Algmate, for the purpose of bonding. 1231179 As a porting agent, a coacervation effect is applied to generate the puppet, and a method of recording the disease with the adhesive production method is a floating agent and there is a ^ ± ^ / ^ 3S supply-drifting agent. Hydroponic plants == control—plant diseases, transparent contact. Another important purpose is to provide a bio-proof household. ▲ Microorganisms to transport two planting agents, which are used to carry the antagonistic floating agents. Sexual effect 丄 'anti-microbial,-floating agent, with-bleaching properties and a material, and a coacervation with the adhesive. The following is a detailed description of the present invention through the following examples. [Embodiment] ^, provides an optimal manufacturing method of the floating agent, the method; 3 agent 1231179 1 provides -A carrier for carrying the antagonist microbiota. in
y 一種_菌，其學名為作為二J :來作，上述漂柯作用劑中之拮抗微生物，用以抗水稻 、、、病原菌，其學名為sc^m· AG-1，以作:p方： 水稻枯紋病之用。 作馬防/ 口 接著’再添加人-造型物f，此造型物f含有—黏合 其^所使㈣黏合物為2 %的藻酸納 田，可與氯化鈣產生凝聚作用，供以作造型之 故斤使用之培養液為一種厚孢子培養液，是用來培養结抗微 劑可月⑹—w>黏带黴菌。^漂i 性作用劑具有—漂浮性f，在本發明中是使用ιί% f之植物性大豆沙拉油，作為漂浮劑。 J Alginate) ’產歧料用，經賴後，即 程#械菌G—ζ·_ vz>my之漂浮性微生物作用劑製造^ 在有_躺此含有料姻G—·_ =作為上述縣性作用射之拮抗微生物，以防 藉以==乃利用此具漂浮性微蝴 曰由:予力/示子一水耕植物系統水面上的特性，而據 ^口試驗。因為水的表面張力及水的危動驅使此严性 ΪΞίίίίίΤίί近，而緊緊吸附在水稻葉^上，藉以佔 存活於葉鞘上，在病原靠近時，隨即進行 til超寄生作用與競爭作用來抑制病原活性，達到病宝 防治功效。其實施過程與結果說明如下。 違i病口 《在第-試驗區，是以水稻插秧後，在水稻 2 ^ ^ ^^m^m Gliocladiurn virens 物作用劑於第一試驗區中的每一小區，每小區以： 1231179 克、，隔週每小區再施用50克。如上述方式，並加上—盔 二治，施之小區以作為對照試驗組及_只施用黏‘黴； 微生物之厚孢子培養液丨次與3次 鹼 才呆作。以上之試驗，各施以四重複試驗，並在在水稻生^期 紀錄紙鹿變化及水稻餘病發生齡，且於㈣時紀錄水稻丄 鮮重，乾種，及測郷患水錄紋狀水麵株上病斑的 病斑咼度，其相對病斑高度，是以下列式子計算而得·· 相對病斑高度 可見病斑最兩點(Heighest Point a Lesion Is Seen) cm (Relative Lesion Heigh) 〇/Γ ^~稻植物體高度~ Xl00〇/〇 在第二試驗區，是以水稻插秧後，在水稻生長第7〇天時， 均巧，黏帚黴菌G/k/—·刪v/聰之漂浮性微生物作用才 於，一試驗區中的每一小區，每小區施用量為1〇〇克，第士 天每小區再施用1〇〇克，第84天每小區再施用5〇克。如上述 ’並力2二無任何防治措施之小區以作為對照試驗組及一 只施用黏帚黴菌G7zbc/iWwm vz>my微生物之厚孢子培養液i 次，3次之試驗來操作。以上之試驗，各施以四重複試驗。並 測量罹患水稻枯紋病之水稻病株上病斑的相對病斑高度，並相 對病斑高度，是以下列式子計算而得： /、 相對病斑高度 可見病斑最高點(Heighest Point a Lesion Is Seen) cm (Relative Lesion Heigh)% 水稻植物體冑度（Plant Height) cm ~ Xl〇〇% >在ΐ/ΓΪ驗區，其試驗結果詳見表一。由表一中可知，經 施用黏帚Μ菌G7/〇c/a必wm Wrms之漂浮性微生物作用劑的相 對病斑高度為13.4 %，施用黏帚黴菌（^〇6也沿·^ vz>e似微生 ，之厚孢子培養液3次的轉病斑紐為23.4 %，施用黏帚黴 ，νζ>^微生物之厚孢子培養液1次的相對病斑 咼度為37.6 %，此三試驗結果經與無任何防治措施之小區的對 照試驗組，其相對病斑高度為41·4 %比較後，在統計上皆呈現 1231179 月”、、頁的差異’顯示该施用黏帚徽菌之漂浮 性微生物作用劑的水稻，其相對病斑高度降低，具有防治效 ，另外、述二種施用黏帚徽菌•謂之漂 浮f生4生物作用劑、施用黏帚黴菌vzyera G4微生 ，之厚孢子培養液3次與施用黏帚黴菌vz>my G_8 ，生物之厚孢子培養液〗次處理之稻榖鮮重分別為8148·8公 斤/公頃、7917.3公斤/公頃與7593·2公斤/公頃重，亦盥盔任 何防治措施之對照_ 5898.7公斤/公頃的產量比較後，^現 日 =差異。相_，前述三種處理之稻榖乾重分別為68524 =斤/公頃、6620.9公斤/公頃與625〇·5公斤/公頃重，亦與對 π、、且之5648公斤/公頃呈現明顯差異。 鱗式„，試驗結果詳見表二。由表二可見，經施用 斑古z聰之漂浮性微生物制劑的相對病 18.75 Gliodadium virens M±4^ .3次的相對病斑高度為13.5 %，施用黏帚_ 麵v_微生物之厚孢子 度為29.5 %，此三試驗結果與轉 ^目^届斑间 驗組，J:相對病斑m二；；0何防，口措知之小區的對照試 ，、不目對届斑回度為47.25 %，經比較後，皆呈 :，顯示該施用黏帚黴菌G"o杨 二 物作用綱水稻，其姆餘高贿低，«卩 =被生 試驗結果 處理方法 施用黏帚黴菌G//octo/_ 漂浮性微生^ 相對病班¢^(%) 13.4 稻穀鮮重ί±ίΙ^Μ) 8148.8 稻穀乾重ί^ί^Ι) 6852.4 10 1231179 施用黏帚徽菌 微生物之厚孢子培養液 3次 23.4 7917.3 6620.9 施用黏帚徽菌G/z_oc/fl^/z·丽 微生物之厚孢子培養液 1次 37.6 7593.2 6250.5 無任何防治措施之對照試驗組 41.4 6898.7 5648.6 (附註：以上，表格一之同一欄内之各項數值，經Duncan，s多 重範圍分析檢測過，在Ρ=0·05下，並無顯著差異(Signiflcantiy Different)存在，其數值足可個別代表其四重複實驗值。）y A kind of bacterium, whose scientific name is to be used as the second J :, the antagonistic microorganisms in the above-mentioned bleaching agent are used to resist rice, rice, and pathogenic bacteria. Its scientific name is sc ^ m · AG-1, as: p side: for rice sheath blight. As a horse defense / mouth, then add a person-modeling object f, which contains-adheses the ^ made by the ㈣ glutinous compound is 2% alginic acid Natian, can produce agglomeration with calcium chloride, for use as The culture medium used for modeling is a thick spore culture medium, which is used to cultivate the anti-micro-agent Kojima-w > viscosus. The bleaching agent has -floating f. In the present invention, a vegetable soybean oil of% f is used as the floating agent. J Alginate) 'For the production of disproportionate materials, after the process, the process #Manufacture of bacteria G-ζ · _ vz &my; floating microbial agents ^ In the presence of _ lie this contains the material marriage G- · _ = as the above county Antagonistic microorganisms that are sexually active, in order to prevent the use of this floating micro-butterfly: Yu Li / Xi Zi-Hydroponic plant system on the water surface characteristics, and according to oral tests. Because of the surface tension of water and the critical movement of water, this strictness is driven close, and it is tightly adsorbed on the rice leaf ^, so as to occupy the leaf sheath. When the pathogen is close, it will then perform til superparasitism and competition to suppress Pathogenic activity, to achieve the efficacy of disease prevention and control. The implementation process and results are described below. "In the first test area, after transplanting rice with rice, Gliocladiurn virens in rice 2 ^ was applied to each plot in the first plot, each plot: 1231179 g, Every other week, another 50 grams were applied. In the same way as above, plus the helmet and two treatments, the Shizhi plot was used as a control test group and _ only applied the slime mold; the thick spore culture solution of the microorganisms and the alkali were left to work. Each of the above tests was performed in quadruplicate, and the changes of paper deer and the remaining age of rice disease were recorded during the rice growth period, and the fresh weight of the rice, dry seeds, and water striatum were recorded at the time of rice growth. The relative spot height of the spot on the water surface plant is calculated by the following formula. The relative spot height is the two most visible spots (Heighest Point a Lesion Is Seen) cm (Relative Lesion) Heigh) 〇 / Γ ^ ~ Rice plant height ~ Xl00 // In the second experimental area, after transplanting rice with rice, on the 70th day of rice growth, it was all coincident. v / Satoshi's floating microbial effect is only applied to each plot in a test area with an application amount of 100 grams per day, 100 grams per day on the first day, and 5 times per day on the 84th day. 〇 克。 0 grams. As described above, the plot without any control measures was used as the control test group and one application of the thick spore culture solution of G7zbc / iWwm vz &my; and three times for the test. Each of the above tests was repeated four times. The relative height of the diseased spot on the rice diseased plant suffering from rice leaf blight was measured, and the relative height of the diseased spot was calculated by the following formula: /, The highest point of the diseased spot can be seen from the relative height of the diseased spot (Heighest Point a Lesion Is Seen) cm (Relative Lesion Heigh)% Rice plant height (Plant Height) cm ~ X100% > In the ΐ / ΓΪ inspection area, the test results are shown in Table 1. It can be known from Table 1 that the relative lesion height of the floating microbial agent of G7 / 〇c / a 必 wm Wrms after application of Gliocladium spp. Is 13.4%, and the application of Gliocladium spp. (^ 〇6also along with ^ vz > e looks like a microbiology. The thickness of the spore culture solution for 3 times is 23.4%. With the application of Gliocladium spp., the relative plaque degree of the thick spore culture solution of the microorganism is 37.6%. Results After comparison with the control test group of the plot without any control measures, the relative lesion height was 41.4%. Statistically, they showed a difference of 1231179 months. The difference between the page and the page shows the floating of the application Rice with a biological microbial agent has a relatively low disease spot and has a control effect. In addition, the two kinds of application of the fungi of the fungus are called floating fsheng 4 biological agents, and the application of the fungus vzyera G4 is slightly thick. The spore culture solution 3 times and the application of Gliocladium vulgaris vz > my G_8, biological thick spore culture solution〗 The fresh weight of rice paddy was 8148 · 8 kg / ha, 7917.3 kg / ha and 7593 · 2 kg / ha respectively. , And the comparison of any control measures of the helmet _ 5898.7 kg / ha After comparison, ^ present day = difference. Relative _, the dry weight of rice paddy in the three kinds of treatments mentioned above were 68524 = kg / ha, 6620.9 kg / ha and 6250.5 kg / ha, respectively. 5648 kg / ha showed a significant difference. The scale type, the test results are shown in Table 2. From Table 2, it can be seen that the relative disease of the floating microbial preparation after the application of Zugu Zicong 18.75 Gliodadium virens M ± 4 ^. 3 times relative The height of the diseased spot is 13.5%, and the thickness of the sporozoites of the viscous _ face v_ microorganism is 29.5%. The results of these three tests and the transfer of the interstitial examination group, J: relative lesion m2; The comparison test of the community-known community was 47.25%, and after comparison, they all showed that: the application of G. slime mold G " o poplar is the key role of rice, and its benefits are high. Low, 卩 卩 = treatment method results of application of Gliocladium spp. G // octo / _ floating microbiotic ^ relative disease class ¢ ^ (%) 13.4 fresh rice weight ί ± ΙΙΜ) 8148.8 dry rice weight ^^ ί ^ Ι) 6852.4 10 1231179 Application of thick spore culture solution of Microbes spp. 23.4 7917.3 6620.9 Application of Microscopy spp. G / z_oc / fl ^ / z · Li thick microorganism spore culture solution once 37.6 7593.2 6250.5 Control group without any control measures 41.4 6898.7 5648.6 (Note: the above, the values in the same column of Table 1, after Duncan, s multiple ranges The analysis has tested that under P = 0.05, there is no significant difference (Signiflcantiy Different), and its value is sufficient to individually represent its quadruplicate experimental value. )
表一.於弟二試驗區施用黏帚黴菌聰vz>e似之漂浮 性微生物作用劑作為水稻生物防治的結果。 處理方法 相對病斑高度(％) 經施用黏帚黴菌丽vz>ew之漂浮性微 18.75 生物作用劑 施用黏帚黴菌vz>e似微生物之厚孢 13.5 子培養液3次 施用黏帚黴菌G7z_(9c/a(iz·画v/rera微生物之厚孢 29.5Table 1. Results of application of a fungal bacterium vz> e like a floating microbial agent in the second test area as a biological control of rice. Treatment method Relative height of lesions (%) After application of Gliocladium vz > ew floating microscopic 18.75 Biological agent application of Gliocladium vz > e Thick microorganism-like spores 13.5 Subculture solution 3 applications of Gliocladium G7z_ ( 9c / a (iz · picture v / rera thick spores of microorganisms 29.5
(附註：以上，表格一之同一攔内之各項數值，經Duncan，s多 重範圍分析檢測過’在p=0.〇5下，並無顯著差異(significantly Different)存在，其數值足可個別代表其四重複實驗值。） 综上所述’可知本發明所揭露之應用此—漂浮性微生物作 ^劑製造方法而得的產品於生物防治上，相對於習知技術，確 貝錢到防雜物病害，降低水雛物體受辆、誠染之病班 11 1231179 相對高度，且可廣泛應用於欲各類欲製造該漂浮性作用劑之方 法及其應用於生物防治方面，充分達成本發明之目的。本發明 得由熟悉此技藝之人士任施匠思而為諸般修飾，然皆不脫如附、 申請專利範圍所欲作用者。(Note: Above, the values in the same block of Table 1 have been detected by Duncan, s multiple range analysis. At p = 0.05, there are no significant differences, and their values are sufficient individually It represents the four repeated experimental values.) In summary, it can be seen that the products disclosed in the present invention using the method of producing floating microorganisms as biological agents are used for biological control. Compared with the conventional technology, Debris disease, reducing the relative height of the water larvae's objects and vehicles that are dyed 11 1231179, and can be widely used in various methods to make the floating agent and its application in biological control, fully cost-effective invention Purpose. The present invention may be modified by anyone skilled in the art, but it is not indispensable to those who are interested in the scope of patent application.
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