玖、發明說明： 【發明所屬之技術領域】 本發明涉及一種光學顯微鏡光纖照明和集光方式之改良，為 一種與光學顯微鏡搭配之共軛焦顯微光纖耦合系統，其應用主要 在於顯微光譜分析或顯微干涉儀。 【先前技術】 現今的光學顯微鏡有許多不同的種類及外型，而技術上常使 用的顯微光譜分析和顯微干涉儀之應用，都必需結合光纖照明和· 集光系統。目前習知的光纖照明與集光技術，可分為二種，第一 種是在光學顯微鏡本體外增搭一額外架設，如圖六所示之先前技 術之一實施例6，即於光學桌上架一支撐桿637，該支撐桿637上 鎖上一平臺638，用以架高光纖照明或集光系統63，以便對齊光 學顯微鏡61之光窗616 ;再於該平臺638上鎖上一固定棒633， 固定棒633上裝置一光纖固定器632並連接光纖631 ;另外/再鎖 上另一固定棒於該平臺638上，固定棒上裝置一集光鏡固定器635 ® 並裝上集光鏡634;將集光鏡634和光纖631對齊並校準後，即可 成為一光纖照明或集光系統63。當作為光纖照明系統時，將該光 纖照明系統63之集光鏡634和光學顯微鏡61之光窗616對齊， 則光源經由該光纖631導入該光纖照明系統63，經集光鏡634平 行擴束後，由該光窗616進入該光學顯微鏡61，即達到照明的目 的。當作為光纖集光系統時，將該光纖集光系統63之集光鏡634 和光學顯微鏡61之光窗616對齊，光學顯k鏡61取樣之光訊號， 經光窗616射出，進入光纖集光系統63，由集光鏡634聚焦後射 入光纖631，以傳達至光譜儀或干涉儀。 第二種習知的光纖照明與集光技術，如圖七所示之先前技術 之一貫施例7，其方法是直接將一光纖π連接到光學顯微鏡η 之光窗713上。當作為光纖照明系統時，則該光纖72另一端連接 光源，使光源透過光纖72導入光學顯微鏡71，達成照明之目的。 當作為光纖集光系統時，則該光纖71另一端連接顯微光譜儀或顯 微干涉儀，則光學顯微鏡71取樣之光訊號，可經光纖72傳導至 光譜儀或干涉儀。 甶以上描述可知：現今習知的光纖照明與 方式是於光學顯微鏡本體之外，增搭一額外且繁複之架設，浪 光學桌上的可用空間；每當移動整個系統架設時，就必須分解 重新校準光學顯微鏡和光纖照明與集光⑽的架設，過程複雜 費時；在校準架設的過程中，欲將光纖照明與集光系統和光學; 微鏡之光窗對齊，還必須配合該支撐桿及固定棒的長度，然後i 肉眼判斷μ確實對齊，實為—操作以且衫用之方法。而】 -種方式為直接將—光纖連接於光學顯之光窗上，當觀㈣ 品偏離焦平面或稍有傾㈣，會產生健或焦點偏移之的情形 因該光纖位置無法調整，使取得的《大幅衰減或失真，無法3 分利用光纖所具有之共軛焦特性。 為解決上述之問題，本發明設計出一共輛焦顯微光纖麵合系 統，以取代目前光學顯微鏡的光纖照明與集光技術；該共軛焦顯 微光纖耦合系統利用連接基座而和光學顯微鏡連接成一體，能取 得極佳之共軛焦顯微訊號，且當系統必需移動時，更可顯現出其 輕便及易校準之優越性。 【發明内容】 如上所述，本發明為一共軛焦顯微光纖耦合系統，其可改良 光學顯微鏡之光纖照明與集光方式。本發明之共軛焦顯微光纖耦 合系統包含：一雙軸校準座，具有一光纖接頭及二調整鈕，該二 調整鈕可調整光纖接頭垂直於光行進方向之位置；一光纖，連接 並固定於該雙軸校準座之該光纖接頭上；一單軸校準座，具有一 調整鈕，可調整該集光鏡接頭平行於光行進方向的位置；一集光 鏡，固定於該單軸校準座之該集光鏡接頭上，用以聚焦光束或將 光源平行擴束；一連接基座，具有一光孔，貫穿該連接基座，用 以通過光束，且該連接基座之型體，可因光學顯微鏡的光窗設計 不同，而予以變化；一支撐架，用以依序連接並固定該雙軸校準 座、該單軸校準座和該連接基座。整個共軛焦顯微光纖耦合系統 整合完成後，可組合在光學顯微鏡之上光窗及側光窗上，使共辆 焦顯微光纖耦合系統和光學顯微鏡連接成一體。藉由旋轉該雙軸 校準座之二調整鈕，可微調該雙軸校準座之該光纖接頭的位置， 使得該光纖所傳導之入射光，經由該光纖接頭而順利到達該集光 鏡，或者使光學顯微鏡所取樣之光訊號，經由該集光鏡順利到達 該光纖接頭而進人該光纖。此外，藉由旋轉該單㈣準座之該調 整紐’可微調該集光鏡接頭之位置，使得該集光鏡之焦點與光學 顯微鏡之物鏡於觀測樣品上產生之焦點形成共焦。 案务月之共輛焦顯微光纖李禹合系統，用以作為光學顯微鏡 之光纖照明與集m藉由旋轉該雙軸校準座和該單軸校準座 周正、·使得"亥共輛焦顯微光纖ί禺合系統之該集光鏡接頭和光 纖接頭的位置皆可微調，以促成光纖照明與集光系統上的兩集光 鏡之焦點，皆可分別與光學顯微鏡之物鏡於觀測樣品上產生之焦 點達成共焦，使聚焦點上形成清晰之光訊號，達到成像最佳化， 充分利用了光纖所具有之共輛焦特性。當移動聚焦點時，毋需實 際將觀測樣品切片’即可取得觀測樣品中任_點之深淺有序的斷 面之訊號，將這些斷面之光訊號經光纖傳輸至顯微光譜儀，可偵 測光強度及光波長，若經光纖傳輸至顯微干涉儀，則可取得相位 貧訊。此外，由於本案發明之共軛焦顯微光纖耦合系統與光學顯 微鏡組合成一體，不但大幅減少了系統空間，更可快速分解與重 組，具有極佳之機動性。 總之，以該共軛焦顯微光纖耦合系統作為光纖照明及集光系 統，可取得極佳之訊號，並改善目前光纖照明及集光技術繁雜的 缺點。 【貫施方式】 參考圖一和圖二，本發明之共軛焦顯微光纖耦合系統之一實 施例2，其結構如圖二所示，包含··一雙轴校準座22，其具有一 光纖接頭221，及兩調整鈕222，用以調整該光纖接頭221垂直於 光行方向之位置；一光纖21，連接於該雙轴校準座22之該光纖接 頭221上；一單軸校準座25，其具有一集光鏡接頭251，及一調 整鈕252，用以調整該集光鏡接頭251平行於光行進方向之位置； 一集光鏡24，固定在該單轴校準座25之該集光鏡接頭251上，用 以聚焦光束或將光源平行擴束；一連接基座26，具有一光孔265 和一卡榫261，該光孔265貫穿該連接基座之26，用以通過光束， 該卡榫261，用以連接並固定該連接基座26於光學顯微鏡1之上 光窗13和側光窗16上；一支撐架23，用以依序連接並固定該雙 軸校準座22、該單軸校準座25和該連接基座26。該共軛焦顯微 光纖耦合系統之一實施例2整合完成後，將該連接基座26之卡榫 261，分別組合於光學顯微鏡1之側光窗16及上光窗13上，使該 共軛焦顯微光纖耦合系統之一實施例2和光學顯微鏡1形成一 體。當以側光窗16處之共軛焦顯微光纖耦合系統之實施例2作為 光纖照明系統，則上光窗13處之共軛焦顯微光纖耦合系統之實施 例2作為光纖集光系統，反之亦可。藉由旋轉該雙軸校準座22之 該二調整鈕222,微調該雙軸校準座22之該光纖接頭221之位置， 可使得該光纖21所傳導之入射光經由該光纖接頭221而順利到達 該集光鏡24，或者使光學顯微鏡1所取樣之光訊號得以經由該集 光鏡24而順利到達該光纖接頭221，並進入該光纖21。當光源由 1222539 光纖進入光纖照明系統，經側光窗16進入光學顯微鏡1，入射光 之光路15經由分光鏡12到達觀測樣品11及光纖集光系統，旋轉 該單轴校準座25之該調整鈕252,微調該集光鏡接頭251之位置， 使得組合於上光窗13和侧光窗16兩處之該共軛焦顯微光纖耦合 系統之實例2上之兩集光鏡24之焦點，皆可分別與光學顯微鏡1 之物鏡14於觀測樣品11上產生之焦點形成共焦。 參考圖一和圖三，本發明共軛焦顯微光纖耦合系統之一實施 例3，其型體如圖三所示，包含：一雙軸校準座32，具有一光纖 接頭321，兩調整鈕322，用以調整該光纖接頭321垂直於光行進 方向之位置；一光纖31，連接於該雙軸校準座32之該光纖接頭 321上；一連接基座36，其具有一光孔365及複數個連結孔361， 該光孔365貫穿該連接基座36，用以通過光束，該複數個連結孔 361，用於連接並固定該連接基座36於光學顯微鏡1之上光窗13 和側光窗16上；一集光鏡34，固定於光孔365上，用以聚焦光束 或將光源平行擴束；一支撐架33，用以依序連接並固定該雙轴校 準座32和該連接基座36。整個共軛焦顯微光纖耦合系統之實施例 3整合完成後，將該連接基座36之連結孔361，分別組合於光學 顯微鏡1之側光窗16及上光窗13上，使共軛焦顯微光纖耦合系 統之實施例3和光學顯微鏡1連接成一體。將側光窗16處之共軛 焦顯微光纖耦合系統之實施例3為·光纖照明系統，則上光窗處13 之共軛焦顯微光纖耦合系統之實施例3為光纖集光系統，反之亦 a 1222539 可。藉由旋轉該雙軸校準座32之該二調整鈕322，微調該雙軸校 準座32之該光纖接頭321之位置，可使得該光纖31所傳導之入 射光經由該光纖接頭321而順利到達該集光鏡34，或者使光學顯 微鏡1所取樣之光訊號，得以經由該集光鏡34而順利到達該光纖 接頭321，並進入該光纖31。當光源由光纖進入光纖照明系統， 經側光窗16進入光學顯微鏡1，入射光之光路15經由分光鏡12 到達觀測樣品11及光纖集光系統，經由匹配該光纖31數值孔徑 之該集光鏡34聚焦，使側光窗16和上光窗13兩處之共軛焦顯微 光纖耦合系統之實施例3之兩集光鏡34之焦點，皆可分別與光學 顯微鏡1之物鏡14於觀測樣品11上產生之焦點形成共焦。圖三 之共軛焦顯微光纖耦合系統之實施例3之架構，其平行光行進路 線方向之位置固定，構造減少一層，縮減了總體積，可用於光源 為單一波長量測時使用，相較於圖二，為更簡易之一實施例。 圖四為連接基座之一實施例26，該連接基座26包含：一光孔 265，貫穿該連接基座26，用以通過光束，並具有螺紋262，可與 集光鏡連接；複數個凹槽264,可使該共軛焦顯微光纖耦合系統之 支撐架與該連接基座26結合；複數個螺絲孔263，用以固定該支 撐架；一卡榫261，用以連接並固定該連接座基26於光學顯微鏡 之光窗。圖五為連接基座之另一實施例36，包含：一光孔365， 貫穿該連接基座36，用以通過光束，並具有螺紋362，可固定集 光鏡；複數個凹槽364,可使該共軛焦顯微光纖耦合系統之支撐架 12 1222539 與該連接基座36結合；複數個螺絲孔363，用以固定支撐架；複 數個連結孔361，用於連接並固定該連接基座36於光學顯微鏡之 光窗上。連接基座之型體，可隨著光學顯微鏡之光窗設計不同而 予以修改，以符合需求。 以上說明中所述之實施例僅為說明本發明之原理及其功效，而 非限制本發明。因此，習於此技術之人士可在不違本發明之精神 對上述實施例進行修改及變化。本發明之權利範圍應如後述之申 請專利範圍所列。 【圖式簡單說明】 圖一為本發明共軛焦顯微光纖耦合系統與光學顯微鏡之組合圖。 圖二為本發明共軛焦顯微光纖耦合系統之一實施例圖。 圖三為本發明共軛焦顯微光纖耦合系統之一實施例圖。 圖四為連接基座之一實施例圖。 - 圖五為連接基座之一實施例圖。 圖六為本發明共軛焦顯微光纖耦合系統之先前技術之一實施例 圖。 圖七為本發明共軛焦顯微光纖耦合系統之先前技術之一實施例 圖。 【元件符號說明】 1:光學顯微鏡 13 1222539 11 :觀測樣品 12 :分光鏡 13 :上光窗 14 :物鏡 15 :光路 16 :侧光窗 2:共軛焦顯微光纖耦合系統之一實施例 21 :光纖 22 :雙軸校準座 221 :光纖接頭 222 :調整鈕 23 :支撐架 24 :集光鏡 25 :單軸校準座 251 :集光鏡接頭 252 :調整鈕 26:連接基座之一實施例 261 :卡榫 262 :螺紋 263 :螺絲孔 264 :凹槽 - 1222539 265 :光孔 3:共軛焦顯微光纖耦合系統之一實施例 31 :光纖 32 :雙轴校準座 321 :光纖接頭 322 :調整鈕 33 :支撐架 34 :集光鏡 36 :連接基座之一實施例 361 :連結孔 362 :螺紋 363 :螺絲孔 364 :凹槽 365 :光孔 - 6:先前技術之一實施例 61 :光學顯微鏡 616 :光窗 63 :光纖照明或集光系統 631 :光纖 632 :光纖固定器 633 :固定棒 ， 1222539 634 :集光鏡 635 :集光鏡固定器 637 :支撐桿 638 :平臺 7:先前技術之一實施例 71 :光學顯微鏡 713 :光窗 72 :光纖 16说明 Description of the invention: [Technical field to which the invention belongs] The present invention relates to an improvement of the optical fiber illumination and light collection methods of an optical microscope. It is a conjugate focus micro optical fiber coupling system for use with an optical microscope. Its application is mainly in microspectral analysis or Micro Interferometer. [Previous technology] There are many different types and shapes of today's optical microscopes, and the applications of microspectral analysis and microinterferometers that are commonly used in technology must be combined with optical fiber lighting and light collection systems. The current known fiber optic lighting and light collection technologies can be divided into two types. The first is to add an extra erection outside the optical microscope body, as shown in Figure 6, which is one of the previous technologies. A support rod 637 is mounted, and the support rod 637 is locked with a platform 638 for raising the optical fiber lighting or light collecting system 63 so as to align the light window 616 of the optical microscope 61; and then lock a fixing rod on the platform 638 633, a fiber holder 632 is installed on the fixing rod 633 and the optical fiber 631 is connected; in addition, another fixing rod is locked on the platform 638, and a light collecting lens holder 635 ® is installed on the fixing rod and the light collecting lens is installed 634; After aligning and aligning the light collecting mirror 634 and the optical fiber 631, it becomes a fiber optic lighting or light collecting system 63. When used as an optical fiber lighting system, the light collecting mirror 634 of the optical fiber lighting system 63 and the light window 616 of the optical microscope 61 are aligned, and then the light source is introduced into the optical fiber lighting system 63 through the optical fiber 631, and the beam is expanded by the light collecting mirror 634 in parallel. When the light window 616 enters the optical microscope 61, the purpose of illumination is achieved. When used as an optical fiber light collection system, the light collection mirror 634 of the optical fiber light collection system 63 and the light window 616 of the optical microscope 61 are aligned, and the light signal sampled by the optical display k mirror 61 is emitted through the light window 616 to enter the optical fiber light collection The system 63 is focused by the light collecting mirror 634 and then enters the optical fiber 631 for transmission to a spectrometer or an interferometer. The second conventional optical fiber lighting and light collecting technology is shown in FIG. 7 as one of the previous techniques. The method is to directly connect an optical fiber π to the light window 713 of the optical microscope η. When used as an optical fiber lighting system, the other end of the optical fiber 72 is connected to a light source, and the light source is introduced into the optical microscope 71 through the optical fiber 72 to achieve the purpose of illumination. When used as a fiber optic light collection system, the other end of the optical fiber 71 is connected to a micro-spectrometer or a micro-interferometer, and the optical signal sampled by the optical microscope 71 can be transmitted to the spectrometer or the interferometer through the optical fiber 72.甶 The above description shows that the current known fiber optic illumination and method is to add an extra and complicated installation outside the optical microscope body, and the available space on the optical table; whenever the entire system is moved, it must be disassembled and re-assembled. The process of calibrating the optical microscope and the installation of optical fiber lighting and collecting light is complicated and time-consuming; in the process of calibrating and setting up, it is necessary to align the optical window of the optical fiber with the light collecting system and optics; The length of the stick, and then i visually judge that μ is indeed aligned, which is the method of operation. And]-one way is to directly connect-the optical fiber to the light window of the optical display. When the viewing product is deviated from the focal plane or tilted slightly, it will cause a health or focus shift. Because the position of the optical fiber cannot be adjusted, Obtained the "significant attenuation or distortion, 3 points can not take advantage of the conjugate focus characteristics of optical fibers. In order to solve the above-mentioned problems, the present invention devises a total focusing micro-fiber surface-bonding system to replace the current optical fiber optical illumination and light collecting technology; the conjugate focusing micro-fiber coupling system is connected to the optical microscope by using a connection base, Can obtain excellent conjugate focus microscopy signals, and when the system must be moved, it can also show its superiority in lightness and easy calibration. [Summary of the Invention] As mentioned above, the present invention is a conjugate focal microfiber coupling system, which can improve the optical fiber illumination and light collection methods of an optical microscope. The conjugate focus micro-optical fiber coupling system of the present invention includes: a dual-axis calibration base with an optical fiber connector and two adjustment buttons, the two adjustment buttons can adjust the position of the optical fiber connector perpendicular to the direction of travel of the light; an optical fiber, connected and fixed to the On the optical fiber connector of the dual-axis calibration base; a single-axis calibration base with an adjusting knob to adjust the position of the light collecting lens connector parallel to the light traveling direction; a light collecting lens fixed to the single-axis calibration base A light collecting lens connector is used to focus the light beam or expand the light source in parallel; a connection base has a light hole penetrating the connection base for passing the light beam, and the shape of the connection base can be adjusted by optical The design of the light window of the microscope is different and is changed; a support frame is used to sequentially connect and fix the two-axis calibration base, the single-axis calibration base and the connection base. After the integration of the entire conjugate focus micro fiber coupling system is completed, it can be combined on the light window and side light window above the optical microscope, so that the confocal micro fiber coupling system and the optical microscope can be connected into one. By rotating the two adjusting knobs of the two-axis calibration base, the position of the fiber connector of the two-axis calibration base can be fine-tuned, so that the incident light transmitted by the optical fiber can smoothly reach the light collecting mirror through the fiber connector, or The optical signal sampled by the optical microscope reaches the optical fiber connector through the light collecting lens and enters the optical fiber. In addition, by rotating the adjustment button 'of the single-mirror mount, the position of the light collecting lens connector can be fine-tuned so that the focus of the light collecting lens and the focus generated by the objective lens of the optical microscope on the observation sample form a confocal. The co-focused micro-optical fiber Li Yuhe system is used to illuminate the optical microscope and set the optical axis by rotating the dual-axis calibration base and the single-axis calibration base. The positions of the light collecting lens connector and the optical fiber connector of the system can be fine-tuned to promote the focus of the two light collecting mirrors on the optical fiber lighting and light collecting system, which can be shared with the focus generated by the objective lens of the optical microscope on the observation sample. Focusing, so that a clear light signal is formed at the focal point, and imaging optimization is achieved, making full use of the co-focusing characteristics of optical fibers. When the focus point is moved, there is no need to actually slice the observation sample to obtain the signals of the order of depth, depth, and order of any _points in the observation sample. The optical signals of these sections are transmitted to the microspectrometer through the optical fiber. If the photometric intensity and light wavelength are transmitted to the micro interferometer via the optical fiber, phase leanness can be obtained. In addition, since the conjugate focus micro-fiber coupling system and optical microscope of the present invention are combined into one body, not only the system space is greatly reduced, but it can also be quickly disassembled and reassembled, which has excellent maneuverability. In short, using this conjugate focus micro-fiber coupling system as a fiber optic lighting and light collection system can obtain excellent signals and improve the current shortcomings of the complicated optical fiber lighting and light collection technology. [Performance Mode] Referring to Figures 1 and 2, a second embodiment of the conjugate focal microfiber optical fiber coupling system of the present invention has a structure as shown in Figure 2, including a two-axis calibration base 22, which has an optical fiber connector 221, and two adjusting buttons 222 for adjusting the position of the optical fiber connector 221 perpendicular to the direction of light travel; an optical fiber 21 connected to the optical fiber connector 221 of the dual-axis calibration base 22; a single-axis calibration base 25, which A light collecting lens connector 251 and an adjusting button 252 are used to adjust the position of the light collecting lens connector 251 parallel to the light traveling direction; a light collecting lens 24 is fixed to the light collecting lens of the single-axis calibration base 25 The connector 251 is used to focus the light beam or expand the light source in parallel. A connection base 26 has a light hole 265 and a tenon 261. The light hole 265 penetrates the connection base 26 to pass the light beam. A tenon 261 is used to connect and fix the connection base 26 to the light window 13 and the side light window 16 on the optical microscope 1; a support frame 23 is used to sequentially connect and fix the two-axis calibration base 22, the The uniaxial calibration base 25 and the connection base 26. After the integration of Embodiment 2 of one of the conjugate focus micro fiber coupling systems is completed, the tenons 261 of the connection base 26 are respectively assembled on the side light window 16 and the upper light window 13 of the optical microscope 1 to make the conjugate focus microscope One embodiment 2 of the fiber coupling system and the optical microscope 1 are integrated. When the second embodiment of the conjugate focus micro-optical fiber coupling system at the side light window 16 is used as the optical fiber lighting system, the second embodiment of the conjugate focus micro-optical fiber coupling system at the upper light window 13 is used as the optical fiber light collection system, and vice versa. By rotating the two adjustment buttons 222 of the dual-axis calibration base 22 and finely adjusting the position of the optical fiber connector 221 of the dual-axis calibration base 22, the incident light transmitted by the optical fiber 21 can smoothly reach the optical fiber through the optical fiber connector 221 The light collecting mirror 24, or the optical signal sampled by the optical microscope 1 can pass through the light collecting mirror 24 to reach the optical fiber connector 221 smoothly, and enter the optical fiber 21. When the light source enters the optical fiber lighting system through the 1222539 fiber, enters the optical microscope 1 through the side light window 16, and the optical path 15 of the incident light reaches the observation sample 11 and the optical fiber light collecting system through the beam splitter 12, and rotates the adjustment button of the single-axis calibration base 25 252, finely adjust the position of the light collecting lens connector 251, so that the focal points of the two light collecting mirrors 24 on the conjugate focus micro-fiber coupling system example 2 combined in the upper light window 13 and the side light window 16 can be respectively Confocal with the focal point produced by the objective lens 14 of the optical microscope 1 on the observation sample 11. Referring to FIG. 1 and FIG. 3, a third embodiment of the conjugate focus micro-optical fiber coupling system of the present invention, as shown in FIG. 3, includes: a biaxial calibration base 32, an optical fiber connector 321, and two adjustment buttons 322, Used to adjust the position of the optical fiber connector 321 perpendicular to the direction of light travel; an optical fiber 31 connected to the optical fiber connector 321 of the biaxial calibration base 32; a connection base 36 having a light hole 365 and a plurality of connections A hole 361, the light hole 365 penetrates the connection base 36 for passing the light beam, and the plurality of connection holes 361 are used to connect and fix the connection base 36 to the light window 13 and the side light window 16 on the optical microscope 1. A light collecting lens 34 is fixed on the light hole 365 to focus the light beam or expand the light source in parallel; a support frame 33 is used to sequentially connect and fix the two-axis calibration base 32 and the connection base 36 . After the integration of Embodiment 3 of the entire conjugate focus micro fiber coupling system is completed, the connection holes 361 of the connection base 36 are respectively assembled on the side light window 16 and the upper light window 13 of the optical microscope 1 to couple the conjugate focus micro fiber The third embodiment of the system is integrated with the optical microscope 1. The third embodiment of the conjugate focus micro-optical fiber coupling system at the side light window 16 is a fiber optic lighting system, and the third embodiment of the conjugate focus micro-optical fiber coupling system at the top light window 13 is an optical fiber light collection system, and vice versa a 1222539 can. By rotating the two adjustment buttons 322 of the dual-axis calibration base 32 and fine-adjusting the position of the optical fiber connector 321 of the dual-axis calibration base 32, the incident light transmitted by the optical fiber 31 can smoothly reach the via the optical fiber connector 321. The light collecting mirror 34 or the optical signal sampled by the optical microscope 1 can pass through the light collecting mirror 34 to reach the optical fiber connector 321 smoothly and enter the optical fiber 31. When the light source enters the optical fiber lighting system from the optical fiber, enters the optical microscope 1 through the side light window 16, and the optical path 15 of the incident light reaches the observation sample 11 and the optical fiber light collecting system through the spectroscope 12, and the light collecting lens matching the numerical aperture of the optical fiber 31 Focus 34 so that the focal points of the two focusing lenses 34 of the third embodiment of the conjugate focus micro-fiber coupling system of the side light window 16 and the top light window 13 can be respectively focused on the observation sample 11 with the objective lens 14 of the optical microscope 1 The resulting focus forms a confocal. The structure of the third embodiment of the conjugate focus micro-fiber coupling system shown in FIG. 3 has a fixed position of the direction of travel of the parallel light. The structure is reduced by one layer and the total volume is reduced. Second, it is a simpler embodiment. FIG. 4 is an embodiment 26 of a connection base. The connection base 26 includes: a light hole 265 penetrating the connection base 26 for passing a light beam and having a thread 262 for connection with a light collecting mirror; The groove 264 allows the support frame of the conjugate focus micro-fiber coupling system to be connected to the connection base 26; a plurality of screw holes 263 are used to fix the support frame; a tenon 261 is used to connect and fix the connection base The base 26 is a light window of an optical microscope. FIG. 5 is another embodiment 36 of the connecting base, including: a light hole 365 penetrating through the connecting base 36 for passing the light beam and having a thread 362 to fix the light collecting mirror; a plurality of grooves 364, may The support frame 12 1222539 of the conjugate focal micro fiber coupling system is combined with the connection base 36; a plurality of screw holes 363 are used to fix the support frame; and a plurality of connection holes 361 are used to connect and fix the connection base 36 to On the light window of an optical microscope. The shape of the connection base can be modified according to the design of the light window of the optical microscope to meet the requirements. The embodiments described in the above description are only for explaining the principle of the present invention and its effects, but not for limiting the present invention. Therefore, those skilled in the art can modify and change the above embodiments without departing from the spirit of the present invention. The scope of rights of the present invention should be listed in the scope of patent application mentioned later. [Brief description of the drawings] FIG. 1 is a combination diagram of a conjugate focus micro-fiber coupling system and an optical microscope according to the present invention. FIG. 2 is a diagram of an embodiment of a conjugate focus micro fiber coupling system according to the present invention. FIG. 3 is a diagram of an embodiment of a conjugate focus micro fiber coupling system according to the present invention. FIG. 4 is a diagram of an embodiment of a connection base. -Figure 5 is a diagram of an embodiment of the connection base. FIG. 6 is a diagram of an embodiment of the prior art of the conjugate focus micro fiber coupling system of the present invention. FIG. 7 is a diagram of an embodiment of the prior art of the conjugate focus micro fiber coupling system of the present invention. [Description of component symbols] 1: Optical microscope 13 1222539 11: Observation sample 12: Beamsplitter 13: Upper light window 14: Objective lens 15: Optical path 16: Side light window 2: One of the conjugate focus micro fiber coupling systems Example 21: Optical fiber 22: Dual-axis calibration base 221: Optical fiber connector 222: Adjustment button 23: Support frame 24: Condensing mirror 25: Single-axis calibration base 251: Condensing lens connector 252: Adjustment button 26: One connection base Example 261: Tenon 262: Thread 263: Screw hole 264: Groove-1222539 265: Optical hole 3: One of the conjugate focus micro-fiber coupling systems Example 31: Fiber 32: Biaxial calibration base 321: Fiber connector 322: Adjustment button 33: Support frame 34: Concentrating lens 36: One of the connection bases Example 361: Connecting hole 362: Thread 363: Screw hole 364: Groove 365: Light hole-6: One of the prior art Example 61: Optical microscope 616: Light window 63: fiber optic lighting or light collection system 631: fiber 632: fiber holder 633: fixing rod, 1222539 634: light collector lens 635: light collector holder 637: support rod 638: platform 7: implementation of one of the previous technologies Example 71: Optical microscope 713: Light window 72: Optical fiber 16