TW544516B - Automatic sample analyzer and its components - Google Patents

Abstract

An automatic sample analyzer includes: a pipette, a pipette driving device which moves the pipette to a sample vessel present in a predetermined position to cause the pipette to suck up a sample from the sample vessel, and then moves the pipette to an open vessel provided in another predetermined position to cause the pipette to discharge the sample into the open vessel, and an analyzing section for analyzing the discharged sample, the pipette driving device comprising a vertically movable main arm and an elongated guide arm cantilevered by the main arm and extending horizontally, the guide arm having a smaller flexural rigidity than the main arm, wherein the main arm vertically moves the pipette when the sample is to be sucked up from the sample vessel, and the guide arm guides the pipette to the open vessel and then vertically moves the pipette when the sample is to be discharged into the open vessel.

Description

516 V. Description of the Invention α) I Cross Reference to Related Applications] This case is related to Japanese patent application case No. 2000- 2 7 0 5 4 3 (application date: September 6, 2001), 2000 0 1 -2 7248 3 (application date September 7, 2001), 2 01-2 7 2 484 (application date September 7, 2001), 2 0 1 -2 72 48 5 ( Application date: September 7, 2001), 2 0 2 2 7248 6 (Chinese request date: September 7, 2001), 2 0 0 2 2 7 248 7 (Application date: 2 0 1 1 year) September 7), 2000 1 -2 75 3 7 5 (application date September 11, 2001), 2 0 1 -2 7 53 8 5 (application date 2 September 2011 Said), 20 (H-2 753 9 7 (application date September 11, 2001), 2 01-3 5 5 0 9 3 (application date 2001 January 20, 2001), 2 0 0 1 -3 6 2 9 79 (application date: November 28, 2001), 2 0 2-1 0 8 1 1 3 (application date: April 10, 2002), and 2002-126640 (application date of April 26, 2002), and 02 02-1 2 6 640 (application date of April 26, 2002), in accordance with 351]% §119 I request its priority application , The full text of these applications is incorporated herein by reference. [ Bright background] The technical field to which the invention belongs The present invention relates to a kind of sample analysis device and its components, in particular, analysis device. 7 samples of the situation, even small-scale sample points 2. Prior technology is known to date The technique is as follows. • Small automatic analysis, no more than one person, one platform, and its periphery is equidistant = one f valley disc, which has an inverted I disc holding multiple reaction vessels. 4 = divided 'Transfer individual reaction valleys from the reaction container to sample delivery,

> 13933.ptd Page 12 544516 V. Description of the invention (2) The transfer device to the reagent delivery position and to the optical measurement position, the device that sucks and distributes the required amount of sample to the reaction container, and the optical analysis of the sample of the reaction container Device (for example, refer to Japanese Patent Publication No. 1-948 4 2 (1 9 9 9)); a liquid extraction device adapted to move a pipette relative to an open sample container using the torque of a first motor ; And using the torque of the second motor to move the pipette relative to the closed sample container (for example, refer to US Patent No. 6,171,2 80); • an assembly including a hollow compression that can be compressed and extended in the longitudinal direction; A suction tube which is suitable for being accommodated in a clean room when the clean room is expanded, and is raised by the clean room when the clean room is compressed, and a locking device which is used to lock the clean room in the expanded position (reference International Bulletin No. 9 2/2 2 7 9 8); • a pipette, which includes a hollow tube with one end sealed with a seal, and a suction port located on the side wall of the tube adjacent to the end (eg Reference US Patent No. 5, 9 6 9, 2 7 2); • a pipette, which includes a thin pipette for sucking a liquid sample, and a thin ventilation pipe for ventilation during suction, the suction pipe and the ventilation pipe system Hold in a side-by-side relationship (for example, refer to US Patent Nos. 5,9 6 9, 2 7 2); • a pipette cleaning device, including a pipette, which is generally provided with a liquid sample intake port in the vertical direction The mouth is oriented in the downward direction. An external cleaning element of the pipette has a generally vertical penetrating path and the pipette can be loosely fitted inside the penetrating path. A feeding path is used to feed the cleaning liquid into the pipe.

313933.rtd Page 13 544516 V. Description of the invention (3) A penetrating path and a liquid discharging path for draining the used cleaning waste liquid through the penetrating path. The internal cleaning device of the pipette is connected to the suction pipe for The cleaning liquid is fed into the inside of the pipette. When a cleaning liquid is stored, it is connected to the feed path of the cleaning chamber and the internal cleaning device of the pipette. The suction device is connected to the liquid discharge path of the cleaning chamber to extract the liquid from the suction chamber. Used cleaning waste liquid from a measuring tube, a waste liquid storage chamber which is connected to a drain path for storing the cleaning waste liquid sucked out by a suction device, and a driving device for moving up and down the suction tube of the cleaning room At least one of them changes the positional relationship between the clean room and the pipette, wherein the through path has a smaller diameter portion which is spaced a smaller distance from the pipette, and a larger diameter portion which is provided in the Below the small-diameter portion, and the distance from the pipette is greater than the distance between the smaller-diameter portion and the pipette, wherein the feed path and the drainage path are respectively separated from the larger-diameter portion and the smaller-diameter portion. (For example, refer to U.S. Patent No. 5,59, 2,59, 9); and • a pipette cleaning device, which includes a penetrating path, a pipette is inserted in the penetrating path, and the pipette has a suction The port is arranged at its tip end, a feed path is used to supply cleaning liquid to the through path, and a liquid discharge path is used to discharge the cleaning waste liquid from the through path (for example, refer to U.S. Patent No. 5,5 92, 9 5 No.9). Various types of automatic sample analysis devices such as automatic liquid analysis devices have been proposed. Most recent automatic analysis devices are large in size and operate at high speeds, and can process a large number of samples in a short time. In addition, the operation of the automatic analyzer is complicated, so it is necessary to hire a specific operator to become a regular staff member. Local hospitals and private clinics that do not require frequent clinical analysis often appoint specific analysis centers

313933.ptd Page 14 544516 V. Description of Invention (4) Perform clinical analysis. However, clinical analysis results cannot be obtained immediately in emergency cases. Therefore, there is a need for an automatic sample analysis device that is highly versatile, easy to operate, and compact. [Summary of the Invention] In summary, the purpose of the present invention is to simplify the operation of the automatic sample analysis device, so that doctors and nurses can easily operate the analysis device, reduce the volume and weight of the analysis device, and therefore easily transport the analysis device to diagnostic processing and medical treatment. Handle the location, reduce the noise of the analysis device and use it in a quiet environment, as well as ensure that the security and analysis device is easy to maintain and inspect, and save energy. According to the present invention, an automatic sample analysis device is provided, which includes: a pipette; and a pipette driving device that moves the pipette to a sample container existing at a predetermined position, and allows the pipette to suck a sample from the sample container, and then moves A pipette to an open container provided at another predetermined position to allow the pipette to discharge the sample to the open container; and an analysis section for analyzing the discharged sample; a pipette driving device including a vertical A moving rod; a guide rod having less bending rigidity than the main rod; wherein when the sample is to be sucked by the sample container, the main rod moves the pipette vertically, and the guide rod guides the pipette to the open container, and Then when the sample is to be discharged in the open container, the pipette is moved vertically. [Embodiment] The automatic sample analysis device according to the present invention includes: a pipette; a pipette drive device that moves the pipette to the The sample container at the predetermined position, let the pipette suck the sample from the sample container, and then move the pipette to the open container set at another predetermined position, and let the pipette discharge the sample to

313933.ptd Page 15 544516 V. Description of the invention (5) Open container; and an analysis section for analyzing the discharged sample; a pipette driving device includes a main rod that can move in a vertical direction; a guide The rod has less bending rigidity than the main rod; wherein when the sample is to be sucked by the sample container, the main rod moves the pipette vertically, the guide rod guides the pipette to the open container, and then when the sample is to be discharged to the open Move the pipette vertically while holding the container.

According to the present invention, when a sample is to be picked up, the pipette driving device uses the main rod to achieve vertical movement of the pipette relative to the sample container, and uses the guide rod to achieve vertical movement of the pipette relative to the open container. In this way, the rigidity of the guide rod and related components can be reduced, and the weight of the pipette driving device can be reduced. The pipette driving device further includes: a pipette holder for holding the pipette; The horizontal drive section of the tube supports the pipette holder in a vertically sliding manner to drive the pipette holder horizontally; and the vertical drive section of the pipette is used to move the main rod and the guide vertically. The pipette holder is buckled to the main rod in a horizontally detachable manner; wherein the pipette holder can be vertically moved by the main rod when snapped to the main rod, and The lever can be moved vertically when the lever is removed.

The pipette holder may have a convex part, and the main rod has a concave part, which can be adjusted to fit the convex part in the horizontal direction. The pipette holder may include a roller that can be engaged with the guide rod to move with the guide rod. The pipette horizontal drive section may include a pipette vertical sliding section, which supports the pipette holder in a vertically slidable manner.

313933.ptd Page i8 544516 V. Description of the invention (6) The sample analysis device may further include a quantitative pump connected to the pipette, which is used by the sample container after the pipette holder is lowered by the main rod. Aspirate the sample and discharge the sample after the pipette holder is removed from the main shaft. The sample container may be a covered sample container. The vertical driving section of the pipette may include a stepping motor as a driving source. When the pipette holder is coupled with the main rod, the former supplies the stepper motor with a driving current for vertically moving the pipette holder. Larger. According to another aspect of the present invention, a pipette driving device is provided, which includes a pipette holder for holding a pipette, and a pipette horizontal driving section, which is vertically adjustable. The slide mode supports the pipette holder to move the pipette holder horizontally; a main rod, the pipette holder is buckled to the main rod in a horizontally detachable manner; a guide rod, which is composed of The main rod extends horizontally; and a pipette vertical driving section is used to vertically move the main rod and the guide rod, wherein the pipette holder is vertically moved by the main rod when it is fastened to the main rod. And when the main lever is removed, the coupling guide moves in the vertical direction. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: a pipette; and a pipette driving device that moves the pipette to a sample container existing at a predetermined position, and allows the pipette to The sample container sucks the sample, and then moves the pipette to an open container set at another predetermined position to allow the pipette to discharge the sample to the open container; and an analysis section for analyzing the discharged sample; the pipette is driven The device includes a vertical slide section of a pipette, which has a pipette holder for holding the pipette and a supporting element which supports the pipette holder in a vertically sliding manner,

313933.ptd page 17 544516 V. Description of the invention (7) A pipette horizontal drive section, the pipette vertical sliding section is replaceably attached to the pipette horizontal drive section, and a A stop element is attached to the vertical slide section of the pipette to prevent the vertical slide of the pipette when the vertical slide section of the pipette is replaced. According to the present invention, the movement of the pipette can be prevented by the stopper element, so the vertical slide section of the pipette held to the pipette can be safely and easily replaced. The stop element can be removably coupled to the pipette holder and supporting element. The vertical slide section of the pipette may include a cleaning section for cleaning the pipette, wherein when the stopper element is attached to the vertical slide section of the pipette, one tip end of the pipette is accommodated in the pipette slide section. Cleaning section. Based on yet another aspect of the present invention, a pipette stop device for a pipette driving device is provided, which includes a vertical slide section of the pipette, which has a pipette central holder for clamping. A holding pipette and a supporting element support the pipette holder in a vertically slidable manner; a horizontal drive section of the pipette, and the vertical sliding section of the pipette is attached to the pipe in a replaceable manner. A horizontal drive section of the pipette, and a stop element attached to the vertical slide section of the pipette to prevent the vertical of the pipette when the vertical slide section of the pipette is replaced: Γ bone movement. According to yet another aspect of the present invention, an automatic sample analysis device is provided. The automatic sample analysis device includes: a body having an opening and covering the main body of the analysis device; a cover for opening and closing the opening; and a sample holder for: The opening is vertically and inwardly arranged to hold the sample container; a coupling element is used to associate the opening of the lid with the rain tilting the sample holder toward the opening and the closing of the associated lid

313933.ptci Page] 8 544516 V. Description of the invention (8) ^ —___ Close and return the sample holder to the upright position. Hold the sample container set in the sample holder and each t component is used to elastically support the container. The sample and analysis section is used to incline the sample holder from the sample holder toward the opening. The sample container is based on the sample holder having the same sample diameter as the sample holder. According to the present invention, the sample container is easily placed on the sample when the lid is opened. The elastic element provided on the rack shaft is easily set on the sample rack by the opposite side holder. The inner diameter of the sample holder is larger than the outer diameter of the holder. The elastic element includes the sample valleys in the lower part of each of the first and the second one. One side of the sample container is elastically clamped. stand by. 1Ping the sample container coaxially with the sample holder The sample analysis device may include a first elastic element to keep away from the heart of the associated cover: J series: the moving elastic element can be centered on a main axis 2 f :: pieces. The bottom of the sample holder is supported by the spindle °° 妾. The associated supporting element is supported by the connection of the supporting elements, and the sample analysis device includes a deflection cover with a deflection direction, and a button complex with 70 N, which is used for the deflection force of the cover opening pressing element. / ,, Λ detachable way, the cover is closed to prevent misalignment. According to another aspect of the present invention, it includes the same frame, which has two kinds of sample containers to set the outer diameter of the sample container in the lower part of the container; and 3 5 The sample container for holding the sample is used to elastically support the sample container and the sample holder coaxially on the opposite sides of the elastic element. One side of the bismuth leather is M3933.ptd Page 19 544516 V. Description of the invention (9) According to the present invention, it includes a housing cover that is directed inwardly to close the door and set the sample holder in the container. The pipette container is used to suck the open container, which is used to insert the sample to analyze the root pipette of the sample rack and the pipette is inserted into the protrusion of the pipette, and the lock lever is included. Use the set to open the sample rack to return to the sample rack to sample the sample, and let it move when the container is open. The sample holding tube is driven by the main rod. There is another sing in the main rod. It should be opened and closed to clamp the sample and tilted back to the upright sample and analyze the predetermined position. The damaged device can be used to vertically close the hole. On the one hand, it provides an automatic sample opening and covers the analysis device; the same frame, which consists of an open container; a coupling element, which faces the opening, and an associated cover. The elastic element is used to bounce and an analysis section is used to drive the pipette. The housing has the opening to hold the sample rack; the container uses the sample to hold the sample and discharges the sample to lock the sample. The sample device can be stably imported, and the tube is driven by the component and its analysis section, and it can be placed in another open container tube to cover the tube to the tube driving device; and a partial pipette is provided by a pre-position device; A lock analysis section of the sample rack 'prevents the pipette from being inserted into the sample aspiration operation, and can be inserted into the coupling hole. Another aspect of the present invention is a movable support containing a pipette. The driving section and the locking device include a locking rod extending downwardly, and a convex member which is covered by a cap when the pipette is inserted into the sample container. On the other hand, a sample rack locking sample rack is provided for holding the sample. Content ^ The critical support for analyzing the vertical system of the mouth is to move the sample above the set and avoid placing the suction parallel inward during the system, and

3l 3933.pld Page 20 544516 V. Description of the invention (ίο) A locking element is used to mechanically prevent the pipette associated with the pipette from being inserted when the pipette is inserted into the sample container held by the sample holder Operate while moving. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: an analysis section including a container for holding a sample and a detector for analyzing sample components contained in the container; a waste liquid chamber for use To store analysis waste liquid, the analysis waste liquid including samples, reagents and diluents; a negative pressure pump is used to apply negative pressure to the waste liquid chamber, and is used to pass the analysis waste liquid from at least the container and the detector; One is sucked out; the negative pressure pump includes an air pump, which has an air inlet and an air outlet, a package cover, which has first and second through holes and covers the air pump, and an extraction pipe, which is composed of The outer side of the encapsulation cover extends to communicate with the air inlet through the first through hole, and a mute ventilation pipe is connected to the second through hole and protrudes to the outside. According to the present invention, the negative pressure pump is enclosed in a cover, and has an elastic ventilation pipe, thereby being able to effectively mute. The analysis device includes a flexible support base that supports the air pump. The analysis device includes a sensor for sensing the negative pressure applied to the waste liquid chamber, and a control section for controlling the negative pressure pump to adjust the negative pressure to a predetermined pressure range. The predetermined pressure range may be 100 to 300 mm Hg. According to yet another aspect of the present invention, a negative pressure pump is provided, which includes an air pump having an air inlet and an air outlet, and a cover, which has first and second through holes and covers the air pump. An extraction tube extends from the outside of the cover and communicates with the air inlet through the first through hole, and a static

313933.pi a Page 21

544516 V. Description of the invention (11) The sound ventilation pipe is connected to the second through hole and protrudes to the outside. Based on yet another aspect of the present invention, a sample analysis device is provided, which includes a pipette; a liquid mixing container is set at a predetermined position; a sample supply section is used to suck a sample into the pipette and supply the sample with liquid A mixing container; a diluent supply section for supplying a diluent to a liquid mixing container; and a sample analysis section for analyzing a sample diluted with the diluent; wherein the liquid mixing container is composed of a chemically resistant resin And has a thick 4 dagger inside. The sample supply section may include a pipette driving device, which moves the pipette to a sample container located at another predetermined position, allows the pipette to suck a sample from the sample container, and then moves the pipette to the liquid mixing container. Let the pipette discharge the sample into the liquid mixing container. In addition, the 'sample supply section may include a negative pressure supply section which is used to apply negative pressure to the liquid mixing container 俾 suck the sample into the liquid mixing container. In this case, the sample to be supplied to the liquid mixing container is preferably quantified by a quantification device such as a sampling valve. Containers made of chemically resistant resins typically have smooth inner surfaces and are therefore highly water repellent, in other words, less wettable. When the sample remaining on the bottom of the container is mixed with the diluent supplied to the container along the inner surface of the container, the diluent easily remains on the inner surface in the form of water droplets. Therefore, the amount of diluent in the mixed sample is relatively reduced, resulting in inaccurate dilution. This reduces the accuracy of the sample analysis. Generally, the wettability of the resin surface is determined by the surface chemical composition, the type and number of functional groups exposed to the surface, the acidic or basic properties of the surface, the surface crystallinity, and the surface roughness.

313 ^) 33.ptii page 22 544516 V. Description of the invention (12) The present invention shows that the wettability of the inner surface of the container can be improved by roughening the inner surface. In other words, the roughening of the inner surface of the container can prevent the supplied diluent from remaining on the inner surface when the sample is mixed with the diluent, so the sample can be diluted with improved dilution accuracy. This improves analysis accuracy. In the present invention, based on experiments examining the relationship between surface roughness and wettability, it has been found that if the surface has an arithmetic average surface roughness Ra of not less than 0.16 micrometers, the surface wettability will not adversely affect the accuracy of dilution. It was also found that the upper limit of the roughness Ra was preferably about 0.65 m. The roughening of the inner surface of the container is achieved, for example, in the following manner. A round rod having an outer diameter smaller than the inner diameter of the container was attached to the ball mill chuck, and a pad of sandpaper cushioned with sponge was wrapped on the far side of the round rod, so the outer diameter of the resulting round rod became slightly larger than the inner diameter of the container. When the rod is rotated, the distal end of the rod is progressively inserted into the container, so that the inner surface of the container is roughened by sandpaper. Sandpaper can be used for 400 to 15 00 sandpaper. Instead of the aforementioned sandpaper, a soft leather with sponge (model 3 2 0, available from Sumitomo 3M) can be used. The thickness Ra (micron) is defined here as calculated from the following expression (refer to JISB 0 6 0 1), where the part of the thickness curve with the reference length m is cut along the average line and expressed as y = f (X), The average line is taken as the X-axis, and the vertical amplitude is taken as the Y-axis: m: The reference length roughness curve is defined here as a phase-compensated high-band filter.

313933.pid Page 23 544516 V. Description of the invention (13) The cross section curve is obtained by removing the surface wave component whose wavelength is greater than the predetermined wavelength; the cross section curve is defined here as obtained by vertically cutting the surface portion to be inspected The cross-section side is drawn. In the present invention, the liquid mixing container is manufactured by injection molding a thermoplastic resin having chemical resistance. The materials of the liquid mixing container include, for example: acryl-acrylonitrile-styrene resin; acryl-acrylonitrile-styrene resin / polyamide blend polymer; acryl-acrylonitrile-styrene resin / polycarbonate blend Polymer; acrylic-butadiene_styrene resin;

Acrylonitrile-butadiene-styrene resin / blended polymer; acrylonitrile-butadiene-styrene resin / polyvinyl chloride blended polymer; acrylonitrile-butadiene-styrene resin / polyamide blend Polymer; acrylonitrile-butadiene-styrene resin / polybutylene terephthalate blend polymer; acrylonitrile-butadiene-styrene resin / methacrylate resin blend polymer Acrylonitrile-butadiene-styrene resin / polycarbonate blend polymer

Modified acrylonitrile-butadiene-styrene resin; acrylonitrile-chlorinated polyethylene-styrene resin; acrylonitrile-ethylene propylene rubber-styrene resin; acrylonitrile resin; acrylonitrile-styrene resin;

313933.ptd Page 24 544516 V. Description of the invention (14) Gas 4-polyethylene glycol; ethylene-vinyl alcohol resin; crystalline polymer, styrene-butadiene resin; styrene-maleic acid resin; Biodegradable resin (mainly cellulose acetate); Biodegradable resin (mainly high molecular weight thermoplastic polycaprolactone); Teflon; Tetrafluoroethylene / ethylene resin; Tetrafluoroethylene / hexafluoro Acrylic resin; amorphous gasification resin, tetrafluoroethylene / perfluoroalkoxyethylene resin; polytetrafluoroethylene; polyvinylidene fluoride, modified polytetrafluoroethylene; tetrafluoroethylene-hexafluoropropylene / Vinylidene fluoride blend polymer; Tetrafluoroethylene / polypropylene blend polymer; Polyamide 1 1; Polyamide 12 2; Polyamide 4 0; Polyamide-acrylonitrile-butadiene- Styrene blend polymer; Polyamide-maleimide-styrene resin blend polymer; Polyamide-polypropylene blend polymer; Polyamide 6;

313933.pid Page 25 544516 V. Description of the invention (15) Polyamine 6 / amorphous polyolefin blend polymer; Polyamide 6 / special rubber blend polymer; Polyamine 6. 6 6; Poly Ammonium 6 1 10; Polyamine 6 6; Modified Polyamine 6 6; Polyamide 6 6 / thermoplastic elastomer blend polymer; Polyamide 6 T; Amorphous polyamine, Polyamide MXD6 ;

Polypropylene self-identification; Polyamidamine imine; Polyacrylate; Polyaryl maple; Thermoplastic polyimide; Polycyclohexanedimethylene terephthalate; High density polyethylene; Low density Polyethylene; very high density polyethylene; polyetheretherketone;

Polyetherimide; Polyethenyl naphthalate; Polyether nitrile; Polyether maple; Polyethylene terephthalate;

313933.ptd Page 26 544516 V. Description of the invention (16) Polyvinyl chloride, modified polyvinyl chloride; polyvinyl chloride / acrylonitrile-butadiene blend polymer; polybenzimidazole; poly Polybutylene terephthalate; Polybutylene terephthalate-acrylonitrile-butadiene-styrene blend polymer; Polymethylmethacrylimide; Polymethylpentene Polycarbonate; Polycarbonate-acrylonitrile-butadiene-styrene blend polymer; Polycarbonate-polyfluorene imine blend polymer; Polycarbonate-polyethylene terephthalate Blended polymer; Amorphous polyolefin; Poly 6 ^, polypropylene; Polypropylene-polyamide blend polymer; Polyxylylenediamine; Polyfluorene; Modified polyphenylene ether; Modified polymer Polyphenylene ether / polyamine blend polymer; Modified polyphenylene ether / polybutylene terephthalate blend polymer; Modified polyphenylene ether / polyphenylene sulfide blend Composite polymer

313933.pid Page 27 544516 V. Description of the invention (17) Modified polyphenylene ether / special rubber blend polymer; polyphenylene sulfide; polyphenylene sulfide / polyamine 6 6 Polymer; general-purpose polystyrene; highly impact-resistant polystyrene; medium-impact polystyrene; modified polystyrene; syndiotactic polystyrene; polysulfide maple; maleic acid Amine-styrene resin; maleimide-styrene / polyamine blend polymer; methacrylic-styrene resin; methacrylic resin; and modified methacrylic resin . According to another aspect of the present invention, a liquid mixing container is provided. The liquid mixing container includes a cylindrical outer surface, a bottom, and a liquid supply port provided near the upper end for supplying liquid to the bottom along its inner surface. The mixing container is made of a dart resistant resin, and its inner surface is roughened. The container can have an open top and can be manufactured by injection molding with a chemical resistant resin. The inner surface of the container preferably has an arithmetic average surface roughness Ra of not less than 0.16 micrometers. Still preferably, the inner surface of the container has an arithmetic average surface roughness Ra of 0.16 micrometers $ {^ $ 0.65 micrometers.

313933.ptd Page 28 544516 V. Description of the invention (18) The resin resistant to 4 knives can be polyetherimine. The container includes a liquid discharge port provided at a bottom thereof for discharging liquid, and the container includes an air supply port provided at a bottom thereof for injecting gas. Based on yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: a liquid suction tube; a certain amount of section for sucking a sample through the liquid suction tube and quantifying the sample; and an analysis section for analyzing the process The quantitative sample; the liquid suction tube comprises an elongated tube having a liquid flow path (that is, a suction path) extending parallel to the axis line inside it, and all grooves are arranged on its outer surface and extend along its longitudinal direction. According to the present invention, when the cap of the capped vacuum blood sampling tube (sample container) is pierced with a liquid suction tube (a pipette), the inside of the enterprise liquid sampling tube is immediately communicated with the atmosphere by a slot having a minimum distance. Therefore, the sample can be smoothly sucked and quantified through the liquid suction tube, so the analysis of the sample can be accurately performed. In addition, the liquid suction tube has a simplified structure and has a notch formed on the outer surface of the tube in the longitudinal direction. Therefore, the slot of the liquid suction tube and the outside can be cleaned at the same time. In the present invention, the slot is preferably extended parallel to the axis of the tube. This makes it easy to form a notch. The cross-section of the slot is preferably enlarged toward the outer surface of the tube. This will prevent the groove from being blocked by the rubber residue and the sample. In addition, the notches preferably have a cross section with a round bottom. This prevents the grooves from being blocked by the covered rubber residue and the sample. The liquid flow path (suction path) is preferably offset from the tube axis. Such a groove can be formed into a larger cross section. Therefore, the area, configuration, and position of the groove section can be more flexible, thereby improving ventilation efficiency and cleaning efficiency.

31 3933.ptd Page 29 544516 V. Description of the invention (19) According to another aspect of the present invention, a liquid suction tube is provided, which comprises an elongated tube having a liquid flow path parallel to its axis at It extends inside the tube and has all grooves arranged on its outer surface to extend longitudinally. In the liquid suction tube, the notch can extend parallel to the tube axis. The cross section of the notch can be enlarged toward the outer surface of the tube. The cross section of the notch has a round bottom. The liquid flow path is offset from the tube axis. Based on yet another aspect of the present invention, an automatic sample analysis device is provided, including: a pipette; an analysis section for analyzing a sample sucked by the pipette; a pipette cleaning device having a cleaner body And a pipette penetrating path passes therethrough, the pipette being inserted from the inlet to the outlet; and a driving device for moving at least one of the pipette and the cleaner body; wherein the suction capacity provided in the cleaner body The pipe through path includes a pipette guide hole provided at an inlet thereof and coaxial with the pipe; and a pipette cleaning hole formed at an outlet thereof and coaxial with the pipe; wherein the pipette cleaning hole has a first The second, third, and third openings are formed on the inner surface from the inlet to the outlet in this order; wherein the cleaner body includes a ventilation path to communicate the first opening and the atmosphere, and a cleaning liquid supply path is connected to the third opening. And a cleaning liquid discharge path which is in communication with the second opening. According to the present invention, the opening communicating with the atmosphere is formed on the inner surface of the pipette penetrating path of the pipette cleaning device, so the cleaning liquid is less likely to stay inside the pipette cleaning device and can effectively clean the pipette. This allows accurate sample analysis. The analysis device further includes: a supply section for supplying cleaning liquid to the cleaning

313933.pid Page 30 544516 V. Description of the invention (20) Liquid supply path; a suction section for extracting cleaning liquid from the cleaning liquid discharge path; and a driver circuit section for driving the supply section and suction Section; wherein the driving device includes a vertical driving section for vertically moving at least one of the pipette and the cleaner body; wherein the driver circuit section moves upwards on the pipette or the cleaner body moves downward At this time, the supply section and the suction section can be driven to clean the outside of the pipette. In addition, the analysis device further includes: a supply section for supplying the cleaning liquid into the pipette; a suction section for sucking the cleaning liquid through the cleaning liquid discharge path; and a driver circuit section for driving the supply section and Suction section; wherein the driving device includes a vertical driving device for vertically moving at least one of the pipette and the cleaner body; wherein the driver circuit section appears at the tip of the pipette when the pipette passes through the path It can drive the supply section and the suction section to clean the inside of the pipette. Based on yet another aspect of the present invention, a pipette cleaning device is provided, which includes: a cleaner body having a pipette penetrating path therethrough, a pipette inserted from an inlet to an outlet; the suction pipe The measuring tube penetrating path includes a pipette guide hole formed on its inlet and coaxial with the tube, and a pipette cleaning hole formed on its exit and coaxial with the tube; the pipette cleaning hole has first, second and first The three openings are formed on the inner surface from the inlet to the outlet in this order; the cleaner body includes a ventilation path to communicate the first opening with the atmosphere, a cleaning liquid supply path connecting the third opening and a cleaning liquid discharge path It is connected to the second opening. The through-path of the pipette has a circular cross-section. The cleaning opening of the pipette includes first and second through-holes which are connected in series from the inlet to the outlet in this order.

313933.ptd Page 31 544516 V. Description of the invention ⑵) The first and second openings are formed on the inner surface of the first through-hole, and the third opening is formed on the inner surface of the second through-hole, where The internal diameter of the pipette penetrating path is sequentially increased according to the pipette hole, the first penetrating hole and the second penetrating hole.

In addition, the pipette through-path may have a circular cross-section, and the pipette cleaning hole may include first, second, and third through-holes connected in series from the inlet to the outlet in this order, where the first and second openings Is formed on the inner surface of the first through-hole, and the third opening is formed on the inner surface of the third through-hole, wherein the diameter of the pipette tube and the second through-hole is smaller than that of the first through-hole and the third through-hole. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: a pipette having a suction port provided at a tip end thereof; a certain amount of section for sucking and quantifying a sample through the pipette; a supply A section is used to supply liquid to the pipette; an analysis section is used to analyze the quantified sample; and a control section is used to control the quantitative section and the supply section; wherein the control section controls the supply section and Fill the suction port of the pipette with liquid before the sample is aspirated.

According to the invention, the suction port of the pipette is filled with liquid before the sample is aspirated. This prevents the sample from entering the suction port when the pipette is inserted into the sample before aspiration. This improves the accuracy of the quantification. The analysis device further includes a cleaning cleaner for cleaning the pipette, wherein the suction port is arranged near the tip of the pipette on the side wall of the pipette, wherein the cleaning cleaner includes a penetration path, and the pipette Inserted through this path, the cleaning liquid supply path is in communication with the through path for supplying cleaning

313933.ptd Page 32 544516 V. Description of the invention (22) Liquid and a cleaning liquid discharge path which communicates with the through path to discharge the cleaning liquid; wherein the cleaning cleaner is provided so that the pipette suction port The angle between the mouth axis and the inlet axis of the cleaning liquid discharge path must be greater than 90 degrees when viewed in the axial direction of the pipette. With this configuration, before the sample is drawn through the pipette, when the pipette is cleaned in a cleaning cleaner, the suction port is not affected by the negative pressure exerted by the cleaning liquid discharge path. Therefore, the liquid filled in the suction port of the pipette will not be sucked out and sent to the cleaning liquid discharge path. The suction port can be kept filled with liquid in this way, so the sample can be accurately quantified. According to yet another aspect of the present invention, a liquid suction device is provided, which includes: a pipette having a suction port arranged on a side wall of the pipe near a tip end thereof; and a suction section for sucking liquid through the pipette And a cleaner for cleaning the pipette; wherein the cleaner includes a penetrating path through which the pipette can be inserted, a cleaning liquid supply path which communicates with the penetrating path for supplying the cleaning liquid, and a cleaning liquid drain The discharge path is in communication with the through path for draining the cleaning liquid; wherein the cleaner is arranged so that the angle between the suction tube suction port port axis and the cleaning liquid discharge path inlet axis is viewed from the direction of the suction tube axis The time series is greater than 90 degrees. The liquid suction device preferably further includes a liquid supply section for supplying liquid into the pipette, wherein the liquid supply section is used to fill the pipette suction port with liquid before the sample is sucked through the pipette. According to yet another aspect of the present invention, a pipette cleaning device is provided, which includes a cleaner for cleaning the pipette. The pipette has a suction port, which is arranged on the side wall of the pipe and near the tip end. Where the cleaner bag

313933.ptd Page 33 544516 V. Description of the invention (23) Contains a penetrating path through which a pipette can be inserted. A cleaning liquid supply path communicates with the penetrating path to supply cleaning liquid and a cleaning liquid discharge. The path is in communication with the through path for draining the cleaning liquid. The cleaner is arranged to allow the suction tube to suck the angle between the mouth axis and the inlet axis of the cleaning liquid when the cleaning fluid is not placed on the path axis. The angle is greater than 90 degrees. Based on yet another aspect of the present invention, a liquid suction device is provided, which includes a pipette having a suction port provided at a slight portion thereof; a suction section for sucking a first liquid through the pipette; The supply section is used to supply the second liquid to the pipette; and a control section is used to control the suction section and the supply section; wherein the control section is a control section for controlling the supply section and before Two liquids fill the suction port of the pipette. The liquid suction device includes a cleaning cleaner for cleaning the pipette, wherein the suction port is arranged on the side wall of the pipette and is located near the tip of the pipette, wherein the cleaner includes a pipette through which the pipette can be inserted. In the through path, a cleaning liquid supply path is in communication with the through path to supply the cleaning liquid, and a cleaning liquid discharge path is in communication with the through path to release the cleaning liquid; wherein the cleaner is arranged so that The angle between the suction port of the suction tube and the inlet axis of the cleaning liquid discharge path is greater than 90 degrees when viewed in the direction of the suction tube axis. According to another aspect of the present invention, an automatic sample analysis device capable of operating in multiple analysis modes is provided. The automatic sample analysis device includes: an analysis mode selection button for selecting one of the analysis modes; The selected analysis mode is the instruction for analysis operation; a color changing section is used to change the color of the start button; a color changing control section is used to control the color changing area

313933.pid Page 34 544516 V. Description of the invention (24) The color of the drive button is changed according to the selected analysis mode; and an analysis section is used to analyze the sample when a command is received from the starter. According to the present invention, the color of the activation button is changed according to the analysis mode selected by the user. In this way, when the user presses the start button to start the analysis, the analysis mode can be confirmed based on the color of the button. This prevents operation errors caused by incorrect mode selection. The analysis device further includes a touch panel input / display section. The analysis mode selection button and the start button are displayed in the input display section. The color changing section can change the color of the display button displayed in the input / display section. Based on yet another specific embodiment of the present invention, an automatic sample analysis device is provided, which includes: an input section; a display section; an analysis section; and a control section for receiving from the input section When the segment is output, the display section and the analysis section are controlled; wherein the display section selectively displays a main screen, and the main day surface indicates the status of the analysis section ready to start analysis; and an analysis day surface displays the borrow analysis section Regain the results obtained from the analysis; during the period from when the analysis results and analysis screens are displayed to the completion of the scheduled analysis in the analysis section, if the input operation is not performed in the input section, the control section displays the analysis displayed in the section The screen switches to the main day surface. According to the present invention, if no analysis operation is performed before the predetermined operation of the analysis device is completed, the analysis day surface is automatically switched to the main day surface. This eliminates the need for users to manually switch between day and night. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: a touch panel input / display section; and a start button displayed on the

313933.pid Page 35 544516 V. Description of the invention (25) The input / display section-analysis section is used to move the analysis device 1 when a start M instruction from the f display is received. A monitoring section is used to monitor the analysis device. And a control section for controlling the display operation of the input / display section, wherein when the monitoring section 1¾ detects an abnormality 5 the control section can eliminate the start of the input / display section: ugly according to another of the present invention An aspect provides a moving sample analysis device including: an analysis section for analyzing a sample, a display section for displaying an analysis result on an analysis screen, and a control section for controlling the display section and the analysis section where the display is Selective display of the first—the first analysis screen—the results of multiple analysis items are displayed in the first font size and a second analysis The first analysis screen analyzes the item a. The analysis result of a larger number of analysis items 0 is displayed in a second font that is smaller than the size of the first font. The analysis device further includes an input section for selective m for analysis to be displayed. Item S is on the first analysis screen. According to yet another aspect of the present invention, a white-moving sample analysis device is provided. A hole a is a sample through which a DC power supply 9 is provided. A constant current circuit is provided by a DC power supply. The supplier supplies a constant current to the sample 1 passing through the hole a. A resistive detection section detects the impedance 9 of the sample passing through the hole α and an analysis section analyzes the sample 9 based on the detected impedance change. The DC power supply includes a Cochlo power supply. The Cochlo power supply includes an oscillation. A switching section electrically road segment region with the region of the cut segment of an oscillation transducer and frequency synchronized intermittent stream m into the straight-m

313933.pid page 36 544516 V. Description of the invention (26) Voltage; and a boost section for boosting the voltage output by the switching circuit section, wherein the switching frequency is 50 to 100 kHz. The DC power supply can be automatically cooled. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: an analysis section; a casing covering the analysis section; and a container casing unit covering the container for containing a supply to be supplied The analysis section or the liquid leaked from the analysis section; a container holder attached to the outside of the casing to hold the container casing unit; wherein the container casing unit includes two large containers and one small container And a container for covering the large and small containers; each of the large containers has a container body for containing fluid, a mouth portion, the flow system flows into and out of the container body through the mouth portion, and a shoulder portion is directed from the mouth portion to the A small container mounting portion is provided at the shoulder portion to receive the small container, and a container side wall extends downward from the shoulder portion; wherein the small container has a container body for containing fluid, a mouth portion, and a flow system passing through the A mouth portion flows through and out of the container body, a shoulder portion extends downward from the mouth portion, a container side wall extends downward from the shoulder portion, and a bottom surface and the bottom surface configuration follow the small container mounting portion; two The large container is assembled and fitted in the cymbal, so the container side wall extends downward from the small container mounting portion of the two large containers, the two container side walls contact each other, and the small container is docked at the small container mounting portion, which is provided On the shoulders above the container side walls of the two large containers that are in contact with each other. According to yet another aspect of the present invention, a container housing unit is provided, which includes: two large containers; a small container; and a stack to cover the large and small containers; wherein each of the large containers has a container body for containing Fluid, a mouth, a flow system flows into and out of the container body through the mouth, and a shoulder system

313933.pld Page 37 544516 V. Description of the invention (27) Extending downward from the mouth, a small container mounting portion is provided on the shoulder to accommodate the + container and a container side wall extends downward from the shoulder ; The small container has a container body for containing fluid, a mouth portion, a flow system flows through and out of the container body through the mouth portion, a shoulder portion extends downward from the mouth portion, and a container side wall extends from the shoulder portion Extending downwards, and a bottom surface The configuration of the bottom surface follows the small container mounting portion; two of the large containers are combined and fit in the pan, so the container side wall extends downward from the small container mounting portion of the two large containers. The side walls of the containers are in contact with each other, and the small container rests on a small container mounting portion, which is provided on a shoulder above the side walls of the two large containers in contact with each other. The shoulders of the two large containers each have a fixing portion for fixing a small container. The fixed part may include the class part respectively arranged on the shoulders of the two large containers, so the small container # is fixed so that the side wall of the dissolver is held by the class part of the two large containers. The two large containers can be respectively formed with convex or concave portions in the step portion, and the small container can be formed in the side wall of the container with the concave portion and the convex portion, which will be combined with the convex portion or the concave portion formed in the large portion of the container. Alternatively, the two large containers each have a convex portion formed on the step portion, and the small container has a concave portion formed on the side wall of the container, and the puppet is formed on the convex portion of the large container step portion. The two large containers have flanges on top of their convex portions, respectively, and will partially overlap the small containers. The two large containers have the same configuration and the same volume. One of the large containers contains the diluent, while the other large container holds the waste liquid. The small container may contain a hemolytic agent. According to another aspect of the present invention, a container mounting method is provided.

313933.pld Page 38 544516 V. Description of the invention (28) This method is used to install and locate two large containers and a small container. Each of the large containers has a container body to hold the fluid. A mouth is formed by the flow system. Flowing into and out of the container body, a shoulder extends downward from the mouth, a small container mounting portion is provided on the shoulder to accommodate the small container, and a container side wall extends downward from the shoulder. The small container has Each container body is used for containing a fluid, a mouth portion, a flow system flowing through and out of the container body through the mouth portion, and a bottom surface and the bottom surface configuration follow the shape of the small container installation portion. The method includes the following steps. The two large containers, the side walls of the containers are in contact with each other; and the small container is docked on the small container mounting portion, which is arranged on the shoulder above the side wall of the container where the two large containers are in contact with each other. Based on yet another aspect of the present invention, a container is provided, which includes a container body for containing fluid; a mouth portion through which fluid flows into and out of the container; a shoulder portion extending downward from the mouth portion; a container The side wall extends downward from the shoulder; and a fixing portion is disposed on the shoulder to fix another container. The fixing portion may include a step portion provided on the shoulder portion, and a convex portion or a concave portion formed on the step portion. Alternatively, the step portion may have a convex portion, and a flange may be provided on the upper portion of the convex portion. According to yet another aspect of the present invention, a small container is provided, which includes a concave portion or a convex portion formed on a side wall of the container, and the convex portions or concave portions of the shoulder portion of the large container are coupled together and provided respectively. According to yet another aspect of the present invention, an automatic sample analysis device is provided, which includes: an analysis section; a casing covering the analysis section, and

313933.ptd Page 39 544516 V. Description of the invention (¾) There is a supply / drain port for ^: The container is attached to the outside of the housing. The square liquid enters the analysis section, and the liquid in the analysis section I # · ； 5 is used to store the supply department to the supply shorea. The flow path connection mechanism is used to connect the outlet port to the main port. Its connection structure is used to connect the container port = mechanism, which can support the vehicle. The connection mechanism for the moving path includes a guide: a guide mechanism, a ... heart: =, and-the mouth is attached to the mouth of the container, and the flow path is different-the mountain has a ^ flow path-the end system port Port; where the guide mechanism is connected to the supply / steam discharge of the housing into the mouth of the container. The support shaft is pivoted at the center, and the guide nozzle lugen structure is used for the flow center to have an end to support the support guides. Among them, the connection container mouth path connecting mechanism according to the present invention is turned, and — flow path , The flow path is connected to the shell as the center of the 4-zone rotating shaft < the attachment guide mechanism includes

Part 40 to Sample :: A mechanism for connecting a flow path including a supply / discharge port for clothing analysis,

ff P / u guide mechanism, which can support # A: connected to the guide mechanism; which, ;; = one end of the system must supply / line enemy to the mouth of the container, the flow path is the other — The guide bow ^ spray 嗔 隹 其中 mouth; the guide mechanism is to enter the mouth of the container with Bevon. To the second analysis of the wall. A second lever and a biasing element are arranged at the first, and pivot at one end from the center, and the second support wheel at the other end of the complex lever is page 544516. V. Description of the invention (30) The center pivot, where the bias The pressure element is disposed between the first lever and the wall of the analysis device to deflect the first lever away from the mouth of the container, wherein the nozzle is attached to the second lever. The guiding mechanism further includes a third lever, which is supported around the support shaft, wherein the third lever is adjusted toward the container mouth portion by pivoting the first lever toward the container mouth portion, which is adjacent to the inner cover provided at the container mouth portion. . Based on yet another aspect of the present invention, a container holder is provided, which includes a support shaft; a guide mechanism is pivoted about the support shaft; and a nozzle is attached to the guide mechanism; wherein The nozzle has a flow path, one end of the flow path can be connected to the mouth of the container, and the other end of the container can be connected to the supply / drain port of the analysis device; wherein the guide mechanism is pivoted around the support shaft. , 俾 guide the nozzle into the mouth of the container. According to yet another aspect of the present invention, a container is provided, which is a first container used in combination with the second and third containers, and includes a first body for containing liquid, a first neck and a shoulder formed on the first container. An upper part of the main body, the first neck portion has a mouth communicating with the inner side of the first body, and a first convex portion protruding from the first neck portion to the first shoulder portion, wherein the second container is formed to communicate with the first A container has the same configuration and includes a second body, a shoulder portion of the second neck, and a second convex portion corresponding to the first container. The third container includes a third body for containing liquid, and the third body has two opposite bodies. The concave portion is formed on the outer side, and a mouth portion and a third shoulder portion are formed on the upper portion of the third body. The mouth portion has a mouth communicating with the inner side of the third body, and the first container and the second container cooperate to hold the third container on the first portion. The first and second shoulders, and the third container are fixed by coupling the first and second convex portions to the opposite concave portion and the third shoulder portion.

313935 pid 苐 page 41 544516 V. Description of the invention (31) The first convex part includes an upper part and a lower part, and the lower part is fitted into one of the two opposite concave parts and the upper part covering the third shoulder part. The container includes an inner cover fitted into the first container mouth, and includes an inner suction tube, wherein the inner cover has a first through-hole for communicating with the inner suction tube, and a second through-hole for release from the first body. The air and pipe are connected to the first through hole and extend to the bottom of the first body. The first body is substantially rectangular. The third body has a substantially flat bottom. Based on yet another aspect of the present invention, a container is provided, which is a third container used in combination with the first and second containers. The third container includes a third body for containing a liquid, and the third body has two opposite sides. The recessed portion is formed on the outer side, and a mouth portion and a third shoulder portion are formed on the upper portion of the third body. The mouth portion has a mouth communicating with the inner side of the third body. The first container includes a first body for receiving a liquid, a first A neck and a shoulder are formed on the upper part of the first body, the first neck has a mouth communicating with the inside of the first body, and a first convex part is protruded from the first neck to the first shoulder, wherein the The second container is formed in the same configuration as the first container and includes a second body, a shoulder portion of the second neck, and a second convex portion corresponding to the first container, and the first container and the second container are joined together to be sandwiched. The third container is held on the first and second shoulders, and the third container is fixed by coupling the first and second convex portions to the opposite concave portion and the third shoulder portion. The container further includes an inner suction tube fitted into the mouth of the third container, wherein the inner cover has a first through hole for communicating with the tube, and a second through hole for releasing air from the third body. Communicates with the first through hole and extends

313933.pid Page 42 544516 V. Inventor Er Ming (32) extends to the bottom of the third body. Based on yet another aspect of the present invention, a flow path connection mechanism is provided, which includes: a lever pivotally mounted on a support member; and a nozzle pivotally mounted on a lever, the nozzle has a proximal end And the distal end, wherein when the lever is pivoted, the proximal end is connected to the outer suction tube, and the distal end is connected to the mouth of the container. The lever includes first, second, and third levers. The first and third levers are pivotally mounted on the support, and the second lever and the nozzle are pivotally mounted on the second lever. When the second, third and third levers are pivoted in the same direction, the first lever leads the distal end of the nozzle into the mouth of the container, and the second and third levers act as the container to keep the nozzle connected to the mouth. The flow path connecting mechanism includes a biasing element for biasing the lever away from the mouth. According to the present invention, the automatic sample analysis device is used for analyzing body fluids (blood, fluid, urine, bone fluid, etc.) of mammals such as humans. The automatic blood analysis device according to one embodiment of the present invention will be described later. The "automatic" blood analysis device here means a blood analysis device that allows a user to set at least one sample container in the analysis device, which can automatically detect the blood sample components contained in the sample container, calculate the value of the analysis item, and output the calculation result . The automatic blood segmentation device is suitable for analyzing blood samples of mammals such as humans. The blood sample here is a human blood sample, and the analysis items (measurement / analysis items) are examples.

313933.ptd Page 43 544516 V. Description of the invention (33) For example, including the number of red blood cells (RBC), the number of white blood cells (WBC), the amount of heme (HGB), the blood volume value (HCT), the number of platelets (PLT), the average body Volume (MCV), mean body hemoglobin (MCH), and mean body hemoglobin concentration (MCHC). As for the measuring principle, sheath current resistance method is preferably used to measure RBC and PLT, resistance method is used to measure WBC and colorimetric method is used to measure HGB. The blood sample to be analyzed is obtained by the individual taking a blood sample into a sample container (blood sample tube). The blood sample may be a whole blood sample or a blood sample initially diluted to a predetermined concentration. Especially when the blood is drawn from an infant, the blood sample is small, so the blood sample is initially diluted to a predetermined concentration (for example, 26 times). Can be used in automatic blood analysis devices as sample containers (blood sample tubes) are commonly used vacuum blood sample tubes (sealed with rubber plugs) and commonly used open blood sample tubes (with an open mouth), the outer diameter of the blood sample tubes is 12 to 15 mm , The length does not exceed 85 mm; the outer diameter of the small-volume blood sample tube is 9 to 11 mm. In the case of whole blood samples, the amount of blood sample required for analysis is, for example, 10 to 15 microliters, and in the case of pre-diluted blood samples, for example, 250 to 350 microliters. The automatic blood analysis device includes a main body and a container housing unit. Preferably, the main system is housed inside the housing, and the container housing unit is movably attached to the side wall of the housing. The main body is included in the display section and is disposed on the front upper portion of the casing. The display section includes an LCD (Liquid Crystal Display Panel) to display the analysis results. If the touch panel and LCD-body forming settings for the analysis conditions are input, the operation capability of the analysis device can be improved and space can be saved. Inside the casing: the same section as the setting section where the user sets the sample container; a detection section where the sample is rationed by the sample container

313933.ptd Page 44 544516 V. Description of the invention (34) The document to be sent and interpreted, and the detection section, which includes the fluid required for the dilution of the sample; the control section, the fluid measurement section, and the power supply; Section, the current voltage is converted to a lower DC output analysis result. It is better to consider the operation and layout of these sections. If the sample setting section cover (sample setting panel) is a cover, approach the sample setting section. In this way, if the detection section is set, check by removing one side wall of the casing. The detection section preferably includes a detector for using the sample to appropriately dilute the blood sample, and the pipette burette used here has a sharp tip if the fluid control section is on the side, or relative to The other side plate of the detection section is easy to connect with the blood component set in the fluid control sample; a fluid control device is used to control the quantitative section of the detection section with an electric control board section, which covers the electric control section And the electric section of the display section is used to press the commercial power supply wheel; and a printer section is used for ease of maintenance and heat generation is placed in the shell, it is easier to install the container than the shell plate An aspirating pipette and suitable for commonly known as piercing can be placed on the back of the fluid section housing JL body. The front of the body is determined to be good. The cover is driven by the right or left approach to the detection tube. This setting is protected near the electromagnetic surface of the control area behind the control area. The side section of the test area device, the liquid component of this container "or the cover. Sections are provided with sections for valves and pumps, as well as maintenance of the unit on the sample side of the user ’s convenience section. A mixing room is quantitatively distributed. “The other side of the“ needle ”is caused by maintenance and inspection. If you want to print and open / close this volume, you can check and remove the blood to check. miscellaneous

3l3933.ptd Page 45 544516

31 j933.pid Page 46 544516 V. Description of the invention (36) A sample setting section 6 is used to receive the sample container, and a detection section 7 is used to distribute the sample quantitatively from the sample container. The sample is diluted and the sample composition is divided. The analysis system is provided inside the right side plate of the casing 2. The fluid control section 8 is a collection of fluid devices, such as fluid valves and pumps, for controlling the fluid in the detection section 7 to distribute and dilute the sample '. The fluid control section 8 is provided inside the left side plate of the casing 2. The electric control board section 9 contains a board, which is installed in the electric control device f to electrically control the detection section 7, the fluid control section 8 and the hidden rice section 3 'The electric control board section The 9 series is arranged on the inside of the side plate behind the casing 2. Power supply section 10 is used to convert commercial AC voltage to DC voltage 'and printer section 11 is used to print the analysis results. Power supply section 10 and printer section 1 1 are set at The inside of the top plate of the casing 2. The right and left panels, the back panel and the top panel are fastened by screws, so it is convenient to access the various sections for maintenance. The power supply section 10 including the heat-generating component is arranged at the uppermost position inside the casing 2, and the ventilator (ventilation hole) 1 2, 1 3 is arranged to surround the power supply section 10 of the casing 2, as described in Section 1. Figure 2 shows. Therefore, the air heated through the power supply section 10 is ventilated through the ventilator 1 2 and 13 and is automatically cooled with air without thermally affecting other components of the analysis device. In other words, the power supply section 10 does not require a forced air cooling device such as a cooling fan, so the size of the analysis device and the noise reduction are reduced. As shown in FIG. 3, the container holder 9500 is attached to the left side of the analysis device body 1, and the ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ + ~ Agent) and a container B 2 for storing waste liquid

544516 V. Description of the invention (37) ^ ~ ^ ^ __ Hold on the container holder 9 50. Sample—Fengshou Nonglong after H. Figure 4 shows the front view of the sample set of the sample setting section 6. The setting office ^ support office U is centered in the direction of the arrow S ^, ί! :: ΐ: 上 弹 方 菁(Not shown in the figure) Yu Jianshen turns to the sample too borrows the spring i arrow τ side Λ turn to win the car is supported by 15 as a relay, and a sample mother Zhongshan u is used to prevent the sample from being set to: V on the edge of the engine 1 17 belongs to the lower edge of Hehong 5 and 4 is provided with a sample holder 18 for a film loss of 4 ^; direction S is opened. Sample setting enemy film 19a, the lower part of this container. Two gripper claws are in between.上方 ;; Ϊ :; the upper part is used to hold and position the sample volume. The sample setting panel 4 horizontal square ^ Λ 9 1 9 | 3 is fixed to 21 respectively. The distal end of the convex part of σ is fixed to the support plate. Figures 5 and 6 are clips. The gripper claw 19a, the claw i9a, the front view and the side view end of the coffee cup, and one: U r has a v-shaped recess σ 22 provided at the bent portion 23 of the far-off gripper claw 19 and the protruded angle Θ. As shown in FIG. 7, the detailed description & coffee 1 9b is held in a symmetrical manner with respect to the center line of the sample holder 18 in the clamping claw ^ placed in the sample container Spl in the sample holder 18 It is elastically clamped between the claws 19a and 19b. Ϊ 之 ϊ 2 Γ 1 9 b are each made of an elastic plate (e.g., thick " millimeters accommodating samples Rong crying ^ k #corner angle 23 of back curved portion Θ can be elastically changed 俾 fit to 15 mm diameter change, so have different Diameter (such as I2 "7 SP 1 can be coaxially clamped to the sample holder 18.

3l3933.ptd Page 48 544516 V. Description of the invention (38) Figure 8 is a cross-sectional view 'showing that a small analytical container SP2 for taking a small amount of blood sample is installed in the sample rack 18. At this time, the sample container sp2 is less than the outer diameter and height of the sample container SP1, so the adapter is put into the '" 样 & 本' frame 18 to compensate. As shown in Fig. 4 'Sensor (light-interferometer) J for opening and closing the sample setting panel 4 of the test sample 1. It senses whether the sample container is set in the sample holder ~ sensor (limit switch) J 2, and The test of whether the adaptor AD is used (limit switch) J 3 is set in the sample setting section 6. For example, if the sample is set to be in the direction of the arrow S and the support shaft 14 is the center of the pivot, in this configuration, when the user presses the upper end of the button 5, the ugly 5 is slightly different from the figure The reverse rotation of the front 顼 τ, the lower end of the button 5 and the pawl 17 release the seller. For example, ## Jί 4 马 中心 枢 ’This is opened to 4% of the convex pieces of the sample setting panel 4. The 1 i H heart is rounded to the neighboring branch hunting board 2 1 as shown in Figure 9. In this state, use the container SP 1 to insert the sample rack 18, as described in the section! 〇Figure. : Water When the sample setting insert 17 is closed as shown in Figure 11 and then the person setting 2 ~ Ganruyue 4, the pawl spI is m ΐ, so the sample setting insert 4 remains closed. At this time, the sample container I was coaxially clamped between the clamps 19 & and 19b. _ The surface area is quite large (60 mm x 70 mm). Therefore, the user can still operate the button 5 when the container is used. Shao Yu held the sample haaa as shown in Figure 12. The detection section 7 includes a suction | total section 2 η η, π said Jiuli official horizontal drive zone 00 a summer tube vertical sliding section 300, a Suction | ^ Section 4 0 〇, Japanese ~ ~ Li Liguan vertical drive zone U a combination room 70 and a detector 50. Absorption ~ water-almost moving section

544516 V. Description of the invention (39) Figure 13 shows the front view of the pipe horizontal drive section 2000. Support Ϊ for two, from + drive, car 〇 02 and active block 203 series of rotary type is set in dynamic, wind ^: Ju Rishou belt 2 04 series is stretched between blocks 002 and 203. Main (stepping motor) 205 drive. 2. Guide t m: Motors on the front and rear of the tube. Moving plate stern—upper edge system: close; = lower part. ： Sliding member 209 sliding towards the slender horizontal guide shaft 20 7, ¥ 206, 4-the lower edge is coupled with the timing belt, which can be coupled along the bulge, and the timing belt 2049; The 21 ° system is used by its back side 212 to fix the suction volume 22: the moving plate 208 has screw holes 2 ", sigh & vertical slide 300. Level ί: This Ϊ J ,, column ::? The moving plate 208 can be driven again and again by the drive of the motor 205; Brother 1 4 The picture shows the total suction + for the vertical direction along the first sale head & square ^ See moving 3 area = 3 0 0 front view, the third moving section 3 0 0 includes a guide axis as not, suction The tube slides vertically and a pipette holder 301 is supported vertically by a supporting member 301 and is held vertically by PT ^. It can slide on the guide shaft 30 2 and has a pipette. The pipette holder 3 0 5 water grabbing piece 3 ^^ includes a longitudinal slender guide groove 304. Therefore, the 305 guided by the guide groove 304 is inserted into the guide groove 304 and slides vertically and stably. The last name of this pipette holder 3 0 3 can be passed through the notch at the guide shaft 302 for fixing *] The piece 301 has the notches 3 06 and 3 7 and the screws can be shown consistently. Take the piece 301 to the horizontal moving plate 208, as shown in Figure 13

Page 50 544516 V. Description of the invention (40) The suction tube holder 3 0 3 has a guide roller 3 0 8 which guides the guide rod of the suction tube vertical drive section 4 0 0 (detailed later) ) In cooperation with the guide rod to move the pipette holder 303 vertically up and down. A cleaner (a pipette cleaning device) S, in which a pipette PT is inserted into the cleaner to clean the outside and the inside of the pipette PT, and the cleaner S is provided at the bottom of the support member 301. When the pipette holder 3 0 3 is located at the uppermost position of the supporting member 3 0 1 (at the position shown in FIG. 14), the sharp distal tip of the pipette PT is inserted into the cleaner S. The liquid supply / drain tube connector fixed to the bottom of the support 3 0 1 3 0 9, 3 1 0 and 3 1 1 are connected to the proximal end of the pipette PT through the tube 3 1 2, 3 1 3 and 3 1 4 And cleaner S port. The screws 3 1 5 fixed to the pipette holder 3 0 3 and the screws 3 1 6 fixed to the supporting member 3 〇 1 convex portion 3 1 7 are arranged to fix the spacer plate 3 1 8 as shown in FIG. 16 As shown. As shown in Fig. 16, the intermediate partition 318 is fixed, and the fixed pipette holder 3 0 3 at the top position of the supporting member 3 01 is used to prevent the sharp end of the pipette PT from being withdrawn by the cleaner S. The vertical sliding section of the pipette 3 0 0 is first docked on the horizontal moving plate 2 0 8. As shown in FIG. 13, it is fixed on the spacer plate 3 1 8 with screws 3 1 9 and 3 2 0 ( Fig. 17) After screwing into the screw holes 2 1 1, 2 1 2 through the notches 3 0 6 and 3 0 7, the partition plate 3 1 8 is removed by unscrewing the screws 3 1 5 and 3 1 6. In this way, the vertical slide section 3 0 of the pipette can be safely installed in the horizontal drive section 2 0 of the pipette, and the user will not be injured by the sharp tip of the pipette PT. When the pipette PT is disturbed, such as obstruction, the entire pipette is replaced by the vertical slide section 300. At this time, the spacer plate is used for safe replacement operation.

313933.ptd 苐 5] page 544516 V. Description of the invention

Figures 17 and 18 are Mongolian and straight sliding sections 3 0 0 installed in the ^ present ^ and left side view, showing the pipette vertical, the vertical slide area of the pipette / horizontal drive section 2 0 0 status. For example, 3 0 3a has the shape of a cross section = 3 mm of the main rod at the end of the pipette holder 3 03 (detailed later). Insert the pipette into the vertical drive section 4 0 0 suction side view of the vertical drive section residual section, No. 20 Figure 19 shows the pipette vertical drive & | Drive section 4 η + Figure 19 arrow CC The directional aid is only 4U0 to the left as shown in Figure 19, and it is always seen ® Stretched slender main shaft 4 0 1, ― ^ Vertical drive section 4 0 0 includes a horizontal 4 0 2, and the threaded shaft It consists of #: a threaded shaft 403 extending straight through the main shaft 401, which is fixed to the main shaft and the plate 1 =, rotary support, a screw cap, a & M + wheel 402 for screwing, ~ Bearing, and shaft 4 0 2 are set on the supporting piece 4 丨 2, a slide = piece 4 0 4b, the system 5 is placed on the main pole 4 〇 丨 the left end and slide 4 〇 ^ for sliding 嚅 a for The vertical guide main rod 401 and a pipette up and down motor (stepping motor) 40 5 are fixed to the support plate 412. The pulleys 40 6 and 40 7 are respectively fixed to the upper end of the screw shaft 402 and the output shaft of the motor 405. The timing belt 408 is stretched between the pulleys 406 and 407. Therefore, the main lever 401 is vertically moved by the drive of the motor 405. The top position sensor of the pipette which senses that the main arm 401 reaches the end of the page is on the support plate 41 2. 4 01 right end, the coupled pipette is vertical). The main rod 401 has a cross-shaped concave holder 3 0 3 and a cross-shaped end guide rod 4 0 9 is horizontally fixed to the main rod sliding section 3 0 0 of the guide rail 3 08 (Fig. 18 part 4 1 0 is provided on it). The surface is opposite to the suction tube 544516 V. Description of the invention (42) 3〇3a (Figures 17 and 18). As shown in Figure 20, the pipette is cool 3 0 3 and the end 30 3a moves in the direction of the arrow x. The embedded recess 41〇 has a proper% gap. In this case, the vertical movement force of the main rod is directly transmitted to the pipette state 303. The locking rod 411 extends vertically through the middle of the main rod 401, and the upper end line and the curved portion are connected. Close the main pole 4 01. In this specific embodiment, the main pole 4 〇 丨 is made of aluminum bow man gold (A5 0 52), the cross section is 20 mm x 26 mm, length 108 mm =: rod 4 0 9 The manufacturing method is to fold a steel plate with a thickness of 0.5 mm (35: (: (:) into an open square shape, with a length of 180 mm. The paper is made of a tube with a volume of 4-volume-tube ϋ li m 2 ^ good quantity tube Φ The operation of the driving section $ — — ~~ UiA_l volume i ι until the blood sample is located on the same plane from the center of the sample setting area and the measuring tube PT and the sample volume SP 1; 1 mile, & ri Kubo best customer The screw 4 minimizes the momentum of the mouth. Therefore, when the total suction volume, shaft, and shaft are applied to the suction tube ρ τ up to 40 5 torque can be effectively converted into suction * total & PT When the motor 40 5 descends, Mali g descends, and then the motor 4 05 is driven to lower the u force. The lifting prevention stopper 26 penetrates the through hole 26a, such as the tube PBt passes the sample container and the tube pt substantially reaches the sample container. The bottom of sp 1, as shown in Figure 21, and let the suction container SP1 be a vacuum blood sample with an elephant skin lid ^ shown in Figure 22. If the sample end pierces the rubber cover. When the current from the PT tip of the pipette is fixed and distributed by the drive circuit section SP1, the pipette is directed to the sample rack 1 of ατ6. The sample container holder 3 § 3 0 3 a is inserted into the main product.彳 is the motor 2 0 5 is driven to show the suction; when the suction tube up and down motor 4 0 5 is driven 1 4 4 0, as shown in Figure 20 up and down the pipe top position sensor J4 up and down Move the main rod 40 1 to 3 0 3a to fit in the recess 410, and the threaded shaft 4〇 ^ as shown in Figure 21. Take the end

544516 V. Description of the invention (43) The description of C Valley is provided to the motor 405. When the suction tube ρτ drops and pierces the rubber cover, k provides a large wheel output torque. When the pipette PT is lowered, the locking lever 41 丨 is adjusted to the locking hole 25 provided in the convex part 24 protruding on the inside of the sample setting panel 4, as shown in FIG. 23, so when the sample setting panel 4 When accidentally opened, the pipette fT and the sample container SP i can be prevented from being damaged. If the small sample container sp2 is provided in the sample j 1 8 ′ with an adapter AD interposed therebetween, as shown in FIG. 8, the sample adapter detection sensor J3 is activated. Therefore, the control section 500 (the volume is described in detail later) ice = the drop distance of the pipette PT, which essentially reaches the small sample container SP2 ^ When the suction of blood is completed inside, the pipette ρτ returns to the second figure ==. Although when the pipette PT is removed from the sample container SP1, the 'pipette_ can be used together with the rubber cap stuck to the pipette to prevent the rubber cap from being lifted. Opening a stopper 26 2 0 5 When you and the measuring tube PT return to the position shown in Figure 21, the 'suction tube reciprocates if driven and reverses in the direction of the arrow in Figure 20, f motor

= End of the pipette holder 303, 303a, but 〇 concave T :. Then the pipette upper and lower motors 405 are driven: J: inside the cup 409 2 "1, the guide rod 4 0 9 and the guide roller 30 oz. Are handed to the suction ^ by the main suction pipe PT is lowered and then raised Gao's Wen holding is 303. This way, "Bei 1 Sai Fuyi-younger brothers 2 3 and 2 4 are the detector image and a partially cut side view, respectively. The main part of the detector 50 is made of transparent poly-resin cut from the front view.

544516 V. Description of the invention (44) As shown, the detector 50 includes the first, second and third container chambers 5b 5 2 and 5 3 for containing the analysis liquid. The first container chamber 51 has an upper part which is open to the atmosphere. The first container chamber 51 and the third container chamber 53 are communicated with each other. The ruby orifice disc 5 4 is provided as a grid between the first container chamber 51 and the second container chamber 52, and the disc 54 has an orifice 55 having a diameter of 80 micrometers. The second container chamber 52 is provided with a spray nozzle 56. The spray nozzle 5 6 is supported by a nozzle support member 57 and an electrode 5 8 and extends through the second container chamber 52, with its distal end facing the orifice 5 5 and its tail end communicating with the liquid supply pipe joint 59. An electrode 58 made of stainless steel is exposed inside the second container chamber 52. The detector 50 further includes nozzles 60 and 61 for supplying a diluent and a hemolytic agent to the first container chamber; and a pipe joint 6 3 and 6 4 for supplying and releasing liquid into and out of the second container chamber 5 2 And a liquid discharge pipe joint 65 and a bubble injection pipe joint 66 are provided at the bottom of the third container chamber 53. As shown in FIG. 24, the tester 50 further includes a ship electrode 67 protruding from the first container chamber 5b, and the light emitting diode 68 and the light diode 69 are respectively disposed in the third container chamber. 5 3 Opposite side. The light emitting diode 6 8 emits light with a wavelength of 5 5 5 nm, and the light diode 6 9 detects the intensity of the light transmitted through the third container chamber 5 3. Light-emitting diodes 68 and 69 were used to measure the hemoglobin content (HGB). The electrodes 67, 58 were used to measure the impedance change of the liquid passing through the orifice 55, and the numbers of white blood cells, red blood cells, and platelets were calculated. As described in detail later, the first and third container chambers 5b and 53 are used to prepare white blood cell measurement specimens, and the first and second container chambers 5b and 5 2 are used to calculate the number of white blood cells, platelets, and red blood cells. Returning device) structure

313933.pid Page 55 544516 V. Description of the invention (45) _ 25U) and 25 (b) are the mixing chambers? 〇 之 向 ~ Figure. The mixture 70 includes a container for mixing blood samples. The f and plane 71 are cylindrical, and the top is open to the atmosphere. The second and seventh bowler part 7 2 is installed in the Rong crying part 8 7 1 u 'thinner supply pipe joint ". The upper part of the π u knife 71 is also placed. The liquid mixing money 73, and the remaining liquid from the container part 71 Pipe connection ^ discharge joint (air) is injected into the agitated container part 71 of the total liquid 4 and the bottom of the bubble container chamber 71 1. The g joint 7 5 is set at 79 guan joints 72, 73, 74, 75 respectively It is connected to the liquid port 8 2 a, the liquid discharge port 7 3 a, 7/1 Ώ⑹γ, and the port port knife is connected to the container part 71, and the port port 75a is connected to the pipe joint. Inner peripheral surface of 71 = flooded, ja open 'is used by the upper chamber 70 (detailed later) for soil, such as the spleen. If the diluent is supplied into the mixed liquid supply π, the inner surface of the U section 71 can The coming mixing chamber will be a right dilution / effect clean through Γ. Etheramine injection mold "迕 :: Thermoplastic resin with chemical resistance such as poly surface roughness Ra is " two each: the inner surface of part 71 is roughened Up to the arithmetic average 0 < mountain, V * secret --- release water, 俾 provides a high enough diluent wetting. Qiu, = :, ′, w Beikou 72a The diluent supply container part 7 1 bottom M ^ droplet steroids reside on the inner surface, so the blood sample initially supplied can be accurately diluted a predetermined number of times. α ^ FIG. 38 is a longitudinal sectional view of the pipette PT. The suction tube PT is a stainless steel tube, which has a suction flow path 31, which is coaxially extended, and has a distal end, a sharp f cutting angle a = 30 degrees. When the covered sample container SP1 is used, the lid 刺 is pierced with the distal end. The distal end of the suction path 3 1 is sealed with stainless steel 33

Page 56 544516 V. Description of the invention (46) Seal, suction step 3 2 Open on the side wall of the pipette PT, and its axis extends perpendicular to the axis of the pipette PT. FIG. 26 is a plan view of the cleaner body 80. Figures 27 and 32 are views in the direction of the D-D arrow and the direction of the E-E arrow, respectively. As shown, the cleaner body 80 has a pipette penetrating hole 81 extending in the center thereof, so the pipette PT is vertically inserted into the pipette penetrating hole 81 from the inlet 81a to the outlet 81b. The pipette through-hole 81 has a circular cross section. The pipette penetrating tube L 81 includes a pipette guide hole 82, a first penetrating hole 8 3, and a second penetrating hole 8 4 arranged coaxially in series from the inlet 8 1 a to the outlet 8 1 b in this order. The pipette guide hole 82 has an inner diameter slightly larger than the outer diameter of the pipette PT, and is used to guide the pipette PT so that the axis of the pipette PT is aligned with the first and second through holes 8 3 and 8 4. In terms of it, the first and second through holes 8 3, 8 4 constitute a pipette cleaning port for pipette cleaning. The first opening 8 5 a and the second opening 8 5 b are respectively formed on the entrance side and the exit side of the inner surface of the first through hole 83. The third opening 85c is formed in the second penetration? L 8 4 inside. The cleaner body 80 includes a ventilation path 8 6 a for opening the first opening 8 5 a to the atmosphere (opening to the outside of the cleaner body 80), and a cleaning liquid discharge path 8 7 a which allows the second opening 8 5 b communicates with the cleaning liquid discharge pipe joint 8 7 and the cleaning liquid supply path 8 8 a which allows the third opening 8 5 c to communicate with the cleaning liquid supply pipe joint 8 8. Pipette hole 8 2. The inner diameters 01, 02, and 03 of the first through hole 8 3 and the second through hole 8 4 are set as pipettes? 1 '105%, 115% and 200% of the outer diameter. If the outer diameter of the pipette PT is 2.0 mm, then Dl = 2.1 mm, D2

313933.pid Page 57 544516 V. Description of the invention (47) = 2.3 mm and D3 = 4.0 mm. When the cleaning liquid (thinner in this embodiment) is supplied from the pipe joint 8 8 to the second through-hole 84 and extracted from the pipe joint 87, the pipette PT extends from the upper side through the pipette through-hole 8 1 to the bottom. On the side, as shown in FIG. 34, the cleaning liquid flows into the first through-hole 83 from the second through-hole 84 and makes consistent contact with the outside of the pipette, and is discharged through the pipe joint 87. Therefore, when the pipette PT is moved upward in the arrow direction Z in this state, blood samples and the like adhered to the outside (outer peripheral surface) of the pipette PT are washed and discharged with a cleaning solution. At this time, part of the cleaning liquid is adhered to the pipette PT, and when the pipette PT moves upward, part of the cleaning liquid moves to a higher position than the second opening 85b. In this way, a cleaning liquid may remain on the upper portion of the first through hole 83. However, due to the function of the first opening 8 5 a provided in the first through-hole 83, the upper portion of the first through-hole 83 is maintained at atmospheric pressure, and therefore the cleaning liquid passes the pressure between the first opening 85 a and the second opening 85 b Poorly sucked back into the second opening 85b, and drained into the pipe joint 87 via the second opening 85b. Therefore, the cleaning liquid flowing into the first through hole 83 from the second through hole 84 will not remain on the upper portion of the first through hole 83. In this way, the outside of the pipette PT is effectively cleaned. When the cleaning liquid is supplied from the proximal end of the pipette PT to the distal suction port 32, the tip of the pipette PT is maintained inside the first through-hole 8 3, and a negative pressure is applied by the pipe joint 87, as described in Section 3. As shown in FIG. 6, the cleaning liquid that has passed through the suction pipe 3 1 of the pipette PT is discharged through the suction port 32 of the pipette PT and is sucked into the inside of the pipe joint 8 7 through the second opening 8 5 b, but not Will leak into the second through hole 8 4. In this way, the inside of the pipette PT (that is, the pipette suction flow path 31 and the suction port

544516 V. Description of the invention (48) ^^ _ a 3 2 Inner surface) is cleaned. As shown in the positional relationship between the axial square pipe PT of the suction pipe PT, the cleaner body 80 and the suction position the cleaner body 80, as shown in FIG. As expected, the pipette PT system has a diameter of 8 7 openings, and the axis of 85b is larger than the axis of the bee mouth 32 and the cleaning liquid discharge path. The following phenomenon was observed. Angle Θ. The reason is that from (1) if 0 $ 90 degrees, fill the diluent at > port 32 (detailed later in the summer, the suction path 31 of the tube PT and the suction suction are taken out, when the pipette PT Externally, the negative pressure of the cleaning solution discharge path 8 7 is vacated. Therefore, the blood sample is attached to the blood sample and the port is displayed. However, the number of blood samples drawn into the total Drp is higher than the expected amount. The result is Γ error. Π ★ Cause (2) If Θ > At 90 degrees, the negative pressure of the cleaning liquid discharge path 87a has a direct impact on port 32. Therefore, when the pipette pT2 is externally or internally connected to a for cleaning, there is no gap due to the suction port 32, which can ensure that Accurate quantification. Fig. 33 is a schematic diagram illustrating the structure of the cleaner body 80a. The cleaner body 80 is a modified example corresponding to the cleaner body 80 of Fig. 27. The cleaner body 80a has a pipette. The through hole 81 includes a pipette guide hole 82 and first, second, and third through holes 83a, 89, and 84. The sequence is arranged coaxially from the entrance to the exit. In other words, the first and second through holes 83a, 89 are corresponding to the first through hole 83 in FIG. 27, and the third through holes 84 are corresponding to the second in FIG. 27. Penetration tie 84. As shown in FIG. 33, the second penetration hole 89 has a substance and a suction capacity.

313933.pici Page 59 544516 V. Description of the invention (49) The same inner diameter of the pipe guide hole 82, and the first through hole 83a has an inner diameter larger than that of the second through hole 89, and its inner diameter is substantially equal to The third through hole 8 4 has an inner diameter. In other respects, the cleaner body 80a has substantially the same structure as the cleaner body 80 (FIG. 27), and the suction pipe PT can be cleaned on the cleaner body 80a in the same manner as the cleaner body 80. Blanket _ Another example of burettes __ _ Figure 3 9 is a vertical cross-sectional view showing another example pipette PTa to replace the pipette PT (Figure 38), where a vacuum blood sample tube (using Rubber caps are sealed) as sample containers SP1 and SP2, and Fig. 40 is a sectional view of the pipette PTa. As shown, the pipette PTa is a stainless steel tube, which has a suction flow path (fluid path) 6 0 1 extending in a parallel and skewed relationship with respect to its axis, and a sharp cut angle from a distal tip α = 30 degrees, so the lid of the sample container SP1 or SP2 can be pierced by the distal tip. The distal end of the suction flow path 6 0 1 is sealed with a stainless steel seal 6 0 3. The pipette PTa has a suction port 602 opening on its side wall. The suction port 6 0 2 has an axis extending perpendicular to the axis of the pipette PTa and communicates with the suction flow path 6 0 1. The pipette P T a has all grooves 604 arranged on its outer surface and extending parallel to its axis. The notch 6 0 4 is used to return the sample container to atmospheric pressure immediately after the rubber cap is pierced by the pipette PTa. This ensures that the suction operation of the pipette P T a is smooth and the quantitative accuracy is improved. The slot 6 0 4 has a generally U-shaped cross section, and the widened width is directed toward the outer periphery of the pipette and has a circular bottom. With this kind of section, the cutting groove 6 0 4 will not be blocked by rubber residue and blood, but can be cleaned in

313933.Did Page 60 544516 彡, Description of invention (50) --- Effective cleaning in the urn (Figure 27). Because the axis of the suction flow path 6 Ο 1 is continually μ μ ^ _, the extension is deviated from the axis of the pipette PTa, so the groove 604 can be formed into a larger cross section. κ &? οσ L Q The wearing area and configuration of this slot 6 〇 4 can be more elastically determined to achieve higher efficiency. 41 (a) to 41 (e) are schematic diagrams illustrating the manufacturing process of the pipette pTa shown in Fig. 39. First, a commercially available stainless steel (8118316) tube 610 with an outer diameter of 1.6 mm and an inner diameter of 0.5 mm was cut into a block 612. The cube 612 has a square cross-section with a side length S1 = 1 mm, and a hole 6 0 1. The distance between the edges S2 = 0.55 mm, as shown in Figure 41 (a). Then, as shown in Figure 4 1 (b), the trench 6 1 4 with a width of W 1 mm and a depth of D 1 mm is in a commercially available stainless steel (SUS3 1 6) round rod 6 1 3 with an outer diameter of 2.1 mm. Coaxial forming. Subsequently, as shown in Fig. 4 1 (c), the square block 6 1 2 is embedded in the groove 6 1 4 of the round rod 6 1 3, and the boundary 6 1 5 between the cube 6 1 2 and the round rod 6 1 3 is welded by laser.俾 Combine cubes 6 1 2 and round bars 6 1 3. Then the convex surface part of the square block was polished and removed, and a round tube with an outer diameter of 2.1 mm was obtained. The round tube had a hole 6 0 1 deviated from the tube axis by a distance of 0.4 mm S3, as shown in Figure 41 (d). As shown. A U-shaped notch 6 0 4 having a maximum width of GW 0.5 mm and a depth Gd of 1 mm is formed by grinding into a circular tube, as shown in Fig. 4 (e). The cross-sectional configuration and position of the cutting groove 604 is determined to ensure the most effective ventilation and the most effective cleaning of the cutting groove 604. Then one end of the round tube is cut to a 30 degree angle α, and the end of the hole 601 is made of stainless steel.

3933.pid Page 61 544516 V. Description of the invention (51) 6 0 3 Sealed by welding or silver alloy welding, as shown in Figure 3-9. The suction port 602 is then formed on the side wall of the round tube. In this way, the pipette PTa is manufactured. Negative structure and operation Figure 28 is a partially cutaway front view showing the structure of the negative pressure pump P1 (detailed later) provided in the flow control section 8 (Figure 1). The air pump 90 is mounted on the rubber base 9 1 and is encapsulated with a resin 箧 92. The suction pipe 9 3 of the air pump 90 extends to the outside through the through hole above the 箧 92, and the open end of the ventilation pipe 9 4 of the air pump 90 is fixed to the 箧 92. The pipe joint 9 5 is fitted on the other side of 箧 9 2 through 孑 L, and the muffler vent pipe 9 6 is connected to the pipe joint 9 5. With this configuration, the vibration of the air pump 90 is absorbed by the rubber base 91, and the noise of the air pump 90 is soundproofed by the surrounding body 90. The ventilation noise is silenced by the muffler duct 9 6. This can effectively reduce the noise of the negative pressure pump P 1. The inner diameter and length of the tube 96 are appropriately determined based on experiments to check the effect of noise reduction. In this specific embodiment, a DC air pump with a rated voltage of 12 volts and a rated air output of 2 liters / minute is used as the air pump 90, and an outer diameter of 6.5 mm, an inner diameter of 3 mm, and a length of 300 mm are used. Polysilicon tube as tube 9 6. Fluid Circuit and Circuit Construction Figures 29 and 9 are system diagrams showing a fluid circuit according to this embodiment of the present invention. In this fluid circuit, the fluid device is connected by a fluid supply pipe. The fluid circuit includes a syringe pump SR1 for metering samples from the pipette PT, a syringe pump SR 2 for supplying diluent from the diluent container B 1 to the mixing chamber 70 and the detector 50, and a syringe pump SR3 for The hemolysing agent is supplied from the hemolysing agent container B1 to the detector 50, and a waste liquid chamber WC is used to store the mixture from the mixing chamber 70 and

513933.ptd Page 62 544516 V. Description of the invention (52) Detector 50 0 The discharged waste liquid 'negative pressure pump P 1 is used to apply negative pressure to the waste liquid chamber WC, and a liquid discharge pump P2 is used The waste liquid is discharged from the waste liquid chamber WC to the waste liquid container B 2, an air pump P 3 is used to supply air into the mixing chamber 70 and the detector 50 is used for agitation, and the solenoid valves SV1 to SV4, SV7 to SV14, SV1 6, SV17 and SV20 to SV25 are used to open and close the flow path of the fluid circuit. The syringe pump S R 1 is driven by the syringe pump motor STM4, and the syringe pumps SR2 and SR3 are driven by the syringe pump motor STM5. Stepper motors can be used as syringe motors STM4, STM5. A preferred example of a diluent is CELLPACK available from Sy sme X Company, and a preferred example of a hemolytic agent is STROMATDLYSER WH, which is available from Hess Max . Fig. 30 is a square rose diagram showing a circuit according to a specific embodiment of the present invention. The power supply section 10 converts the voltage supplied by a commercial AC power supply into a DC voltage (12 volts), and supplies it to the control section 500 and the drive circuit section 50 and the control section 50. The microprocessor of ROM and RAM is composed of a 'drive circuit section 501' which includes a driver circuit and an I / 0 port. The input / display section 3 includes a liquid crystal display 3a and a transparent touch panel 3b stacked on the liquid crystal display 3a and is connected to the control section 50. 、、 = Actuator circuit section 5 0 1 pair of insert opening / closing sensors j!, Sample detection sensor J2, sample adapter detection sensor j 3, top position of the pipette jJ 4 Position sensor J in front of the pipette 5. Force sensor j 6 for detecting the negative pressure in the waste liquid chamber WC. A floating sensor J 7 that detects the amount of liquid accumulated in the waste liquid chamber wc. The heme detection section 50 2 composed of the diode 6 8 and the photodiode 6 9 and the resistive detection section composed of the electrodes 5 8 and 6 7 are rounded out for analog-digital conversion, and The converted signal

313933.ptd Page 63 544516 5. The description of the invention (53) paragraph 500b, which processes signals according to a predetermined processing program. Drive section 5 0 0b causes the driver circuit section 50. Based on the household management system, the tube up and down motor 405, the suction tube reciprocating motor 2 (^, Note 1 jet pump ^ dynamic suction injection pump motor STM5, negative pressure) Pump liquid discharge pump p /,:, STM4, solenoid valves SV1 to SV25. Control section 500 control input / display dry P3 and crystal display 3a and print section 1 1 俾 Display and print out eight ° Slave 3 liquid items, analysis results, etc. Control section 5 0 0 into a pack of 7 & pieces, analysis 5 0 0c is used to change the display color of the liquid crystal display 3a, and 2 h H 5 0 0 d In order to control the color-changing section 5 0 0 c (detailed later). The work of the Ba Yi system must be performed by mi shao '. Second, the analysis performed by the blood analysis device shown in Figure 1 1 (a) and 3 1 (b) are illustrated in the flow chart., Η ON (the power supply of the blood analysis device not shown in Figure 2 of Figure 2) is required to prepare for the analysis (including preliminary cleaning operations). When the mile-on-time / period (step S2), the "Ready" message is displayed on the LCD chandelier 3a at M, Shibuya 3. Then the user sets the sample capacity = setting area Segment 6 (Figure 4) (Step S4). When the sample thus set ^ ^ is a whole blood sample, the user selects the whole enterprise mode by using the toucher and $ pendulum of display section 3, and if the sample is When diluting the sample, use I & select the pre-diluted mode (step S5). After pressing f, the user presses the start button of the touch panel flutter (step S6). If the sample container SPm SP2 is not at R ^ 4 After this and / or the sample setting insert 4 is not installed, the 11 sensors 2 and 2 will detect this situation and let the analysis device "," > operate. If the sample container SP1 or SP2 is set and Sample setting insert 4

544516 V. Description of the invention (54) After closing, the analysis device starts to operate. If the whole blood mode is selected (step S7), the whole blood sample is used to select a specimen for measuring the number of red blood cells (r b [) and specimens, and a number of measuring white blood cells (W B C) (steps S8, S9). The WBC measurement specimen prepared in step S9 is used to measure the WBC hemoglobin content s (H GB) (step si 0), and then the measured WBC and hgb are displayed on the liquid crystal display 3a (step SI 1). Then use the RBC test specimen prepared in step S8 to perform RB C measurement, calculate the platelet number (PLT), blood volume value (HCT) and other analysis items including average body volume (Mcv), average body hemoglobin (MCH), And mean body hemoglobin concentration (MCHC). Then the measured RBC and the calculated values of each analysis are displayed on the LCD (steps S13, S14). WB C, RBC and PLT are measured by counting pulses, which indicate the change in impedance between electrodes 5 8 and 67 in the detection state 50. η G B is determined by comparing the absorbance ratio (blank value) of the diluent alone with the absorbance ratio of the wBC measurement specimen measured by photodiode 68. HCT is determined based on the maximum pulse level. The impedance changes between the pulse indicating electrodes 58 and 67. MCV, MCH and MCHC are calculated by the following expression: MC V = (HCT) / (RBC) MCH- (HGB) / (RBC) MCHO (HGB) / (HCT) also calculates the following. LYM%: ratio of small white blood cells to total WBC (estimated to be equivalent to lymphocytes). MXD%: ratio of intermediate white blood cells to total WBC

313933.ptd page 65 544516 V. Description of the invention (55) cells, eosinophils and basophils). NEUT%: ratio of large white blood cells to total WBC (presumed to be equal to neutrophils). L Y M #: Absolute number of small white blood cells (estimated to be equivalent to lymphocytes). Μ X D #: Absolute number of intermediate leukocytes (presumed to be equal to monocytes, eosinophils, and test cells). NE UT # •• Absolute number of large white blood cells (presumed to be equal to neutrophils). RDW-SD: Calculated distribution width of red blood cells, standard deviation RDW-CV: Calculated distribution width of red blood cells, coefficient of variation PDW: Calculated distribution width of platelets MPV: Average gold platelet volume P-LCR: Large white blood cell pair The ratio of total white blood cells is then subjected to a fluid circuit cleaning operation. When the cleaning operation is completed (step S 1 5), the routine returns to step S 3, and "Ready" is displayed on the liquid crystal display 3a, waiting for analysis of the sample. If the pre-diluted mode is selected in step S7, the RBC measurement specimen and the WBC measurement specimen are prepared from the pre-diluted blood sample (steps S16, S17). In this case, a pre-diluted sample is obtained from a preliminary diluted whole blood sample. Therefore, the preliminary dilution factor must be taken into consideration so that the red blood cell measurement specimen and the white blood cell measurement specimen have the same dilution factor as the specimen prepared from the whole blood sample in the whole blood mode. Next, the details of each operation performed in steps S8 to S 1 5 will be described with reference to the flow system diagram shown in FIG. 29. Analysis device is normally closed valve type

313933.ptd Page 66 544516 V. Description of the Invention (56) All valves in the fluid circuit are normally closed. Preliminary S_ 2 丄 (1) The pipette PT moves to the top of the sample rack 丨 8 and then descends, as shown in Figure 2 2 (At this time, the sample container SP1 is not set in the sample setting section ㈠. (2) Turn on Valves SV9 and SV25, the diluent is supplied to the cleaner S by the injection pump SR2, and then discharged to the waste liquid chamber wc. At the same time, the pipette ρτ is raised, as shown in 3, when the tip of the pipette ρτ reaches At the position shown in Figure 35, the suction tube PT stops. This completes the cleaning of the outside of the suction tube ρτ. (3) The valves SV9 and SV25 remain open, and the suction tube ρτ slightly drops to the position shown in Figure 36. Then The valves SV4 and SV10 are opened, and the diluent is supplied to the pipette pt by the syringe pump SR2. At the same time, the diluent discharged from the suction port 32 of the pipette PT is put into the waste liquid chamber WC and the inside of the pipette ρ τ Cleaning. (4) When the valves SV 4, S V1 0 are closed, the diluent flow from the suction port ρ τ suction port 32 to the second opening 85b stops, thereby completing the internal cleaning. At this time, the suction flow path 31 and the suction opening 32 are filled with the diluent. In this aspect, the diluent flow from the third opening 8 5 c to the second opening 8 5 b is continuous, When SV9 and SV2 5 are closed, the flow is stopped. Therefore, the pipette ρτ sucks the cockroach and fills it with # release agent. / Preparation of red blood cell measurement specimen (step S8) SV16, and then closes the valve SV1 6 (1) by negative pressure The pump P1 applies negative pressure to the waste liquid chamber wc, and opens the SV20, thereby discharging the residual SV20 from the detector 50 and the mixing chamber 70. XYou> Night body (2) valve SV2 2 is opened, and the injection pump SR2 is operated The release agent is sucked from the diluent container B1 into the syringe pump s R 2. The suction is performed, whereby the thinner is closed.

313933.ptd Page 67 544516 V. Description of the invention (57) SV22 ° (3) Drop the pipette and insert the soap into the sample container. Then the valves svi 0 and SV8 are opened, and a quantitative (10 microliter) blood sample is injected. The pump SR1 sucks, so the suction pipe ρτ sucks and then closes the valves SV10, SV8. (4) Then raise the pipette ρτ. During the rise, the valves sv9 and SV25 are opened. The diluent is supplied to the cleaner s by the injection pump S R 2, and the bubble is placed in the waste liquid chamber WC to clean the outside of the pipette ρτ. Then, the valves SV9 and δν25 are closed. (3) The valve SVU is opened and the syringe pump SR 2 is operated for discharge, so a predetermined amount (1.3 ml) of the diluent is supplied to the mixing chamber 70. Then close the valve SV1 4.

(6) Suck! The officer moves to a position just above the mixing chamber and then descends. Then the valves SV10 and SV4 are opened, and the syringe pump SR2 is operated to discharge, so the blood sample “10 microliter feathering period is sucked into the pipette” is discharged into the mixing chamber 70. In this way, the blood sample is diluted in the mixing chamber 7 〇Dilution is 130 times, so prepare 1 · 3¾ liters of diluted blood sample after mixing to 70. Then close the valves sv 丨 〇, s V 4. (7) In the steps (2) to (4) of the preliminary cleaning operation After that, the valves SV4 and SV10 'are opened to operate the syringe pump 2 to suck a predetermined amount of air, so as to provide a microliter air gap (air layer) at the suction port 32 of the suction pipe PT. Then the valves SV4 and SV10 are closed.

(8) + valve S V 1 2 is opened 'The air pump ρ 3 is driven to supply air to the mixing chamber 70, whereby the diluted sample is stirred in the mixing chamber 70 by air bubbles. Then stop the air pump P3 and close the valve SV12. (9) The pipette ρ τ drops again to the mixing chamber 70. Then, the valves SV1 0 to SV4 are opened, and the Zhuang shot pump SR2 is pumped to take out a predetermined amount (0 · 5 9¾ liters) of the first ρ all-diluted sample into the pipette. then

544516 V. Description of the invention (58) Close the valves SV10 and SV4. (1 0) When the outside of the pipette PT is cleaned in the preliminary cleaning operation step (2), the pipette PT rises. (1 1) Valve S V 2 0 opens. Then, a negative pressure is applied to the waste liquid chamber WC by the negative pressure pump p i, whereby the residual sample of the mixing chamber 70 is discharged into the waste liquid chamber WC. Then close valve SV20. (1 2) The valve SV 14 is opened and the syringe pump SR2 is operated to discharge, so the diluent is supplied to the mixing chamber 70 by the syringe pump SR 2. The valve SV 1 4 is then closed. Repeat the previous step (1 1). This cleans the mixing chamber. (1 3) The valve SV1 4 is opened, and the syringe pump sr2 is operated to discharge, so the predetermined diluent is initially distributed to the mixing chamber 70 by the syringe pump S R 2. Then the valve SV14 is closed (14) The pipette PT is lowered. Then sv10 and SV4 are turned on, and the syringe pump SR2 is operated to discharge, so 0.59 ml of the first stage retained in the pipette is discharged. 0.2 ml of the diluted sample is discharged into the mixing chamber 70. Then close the valves SV10, SV4. The pipette Pτ is subsequently raised. During the raising period, the outside of the “suction pipe” is cleaned in the manner described above. (1 5) Valve SV1 3 is opened, and the syringe pump SR? Mixing chamber 70, using this method to close the valve and stir by air bubbles-the ratio of π to the hi-hat to identify other knots-hunting this diluted sample 75 times to accept the first-卩 white again. Beidi—stage diluted sample SV 1 3. At this time, the second stage diluted sample is prepared with 7 moves and 0 in the mixing chamber 7 0 to prepare the red blood cell measurement sample and the red measurement 1 body. Shang

313933.ptd Page 69 544516 V. Description of the invention (59) (1) The valve SV 1 3 is opened and the syringe pump SR 2 is operated and discharged, thereby supplying 0 · 5 ml of thinner such as detector 5 0 (preliminary distribution) ). Then close the valve SV 1 3. (2) The pipette PT moves to the upper end of the detector 50 and then descends. Then the valve 8 ¥ 10 and 374 were opened, and the syringe pump 81-2 was operated to discharge, whereby 0.39 ml of the first stage diluted sample was discharged from the pipette PT into the detector 50. Then, the valves SV10 and SV4 (3) are closed and the valve SV2 4 is opened, and the syringe pump SR 3 is operated for suction, whereby the hemolytic agent is sucked from the hemolysing agent container B3 to the syringe pump SR3. Then close valve SV24. (4) The valve SV2 3 is opened, and the syringe pump SR 3 is operated to discharge, thereby supplying 0.5 ml of hemolytic agent to the detector 50. Then close the valve SV23. In this way, 0.39 ml of the diluent, 0.5 ml of the first-stage diluted sample and 0.5 ml of the hemolysing agent were present in the first and third containers 51, 53 of the detector 50. (5) The pipette PT is raised, and the outside and inside of the pipette PT are cleaned in the same manner as the preliminary cleaning operation steps (2) to (4). The suction port 3 of the pipette PT is maintained to be filled with diluent. (6) The valve SV1 1 is opened, and the air pump P3 is operated to supply air into the detector 50 for agitation with air bubbles. Then stop the air pump P 3 and close the valve SV 1 1. This completes the preparation of the leukocyte measurement specimen at 50 of the annoyance device. Measurement of blood cells and heme (step S 10) (1) The valves SV21 and SV18 are opened. A negative pressure is then applied to the waste liquid chamber WC by the negative pressure pump P1, thereby allowing the diluent to flow from the diluent container M to the waste liquid chamber W C through the second container chamber 52 of the detector 50. In this way, the second container chamber 52 is cleaned, and the diluent remains in the second container chamber 52. Then close the valve

313933.ptd Page 70 544516 V. Description of the Invention ⑽) SV2 1, SV18. (2) Open the valve S V1 7 and operate the syringe pump SR 2 for suction, so that the white blood cell measurement specimen flows from the first and third container chambers 5; 1, 53 through the orifice 5 of the detector 50. Zhidi ^ a valley state chamber 5 2 (experienced about 10 seconds). 'Then close the valve S V 1 7. At this time, the control section 5 0 0 detects the changes in the resistance of the electrodes w and 67. Based on the detection results, the number of white blood cells (WBC) is calculated (3). The light emitted by the light-emitting diode 68 is transmitted by the specimen. 1 Light intensity is measured by light-polar body 6 9 彳. Control section 5 0 〇 Based on 彳 彳, 彳? Light intensity meter isomerin (HGB). Immediately after the operation of step (1) white blood cell measurement and measurement, perform a blank measurement of heme (measure the light intensity transmitted by ^% transmission). ^ Measure the red gray ball (step S1 2) (1) Open the valve S V1 6 'Negative pressure is applied to the WC by the negative pressure pump p 1, so put the residual liquid line of the detection 50 into the waste liquid chamber valve SV1 6. Then turn off (2), turn on SV1 3, and operate the syringe pump SR2 to discharge. Shi Xi 丨 Supply the detector 50 to the first and third volume F chambers 51, and thus dilute SV13. H workers to 51,53. Then close the valve (3), open the valves SV21, SV18, and the negative pressure pump pu chamber wc ', so that the diluent container Bi supplies the diluted negative two to two two container chamber 52 for cleaning the second volume cry # 室 52 . f Profit 50 brother SV2 Bu SV18. Monthly work to 52. Then close the valve (4) Open the valve SV and SV3, and operate the injection pump SR2 to take the red blood cell measurement sample from the mixing chamber 70 and take it.

Ms line CL and stay on feed

313933.ptd Page 71 544516 V. Description of the invention (61 丨 material line CL. Then close the valves SV1, SV3. 3 5) Open the valve SV 1 7 and operate the syringe pump SR2 to discharge, so that the diluent is discharged from the third container The chamber 53 flows into the first container chamber 51 through the orifice 5 5 of the detector 50. (6) During this period, the valve SV7 is kept open, and the injection pump SR1 is operated to discharge the red blood cell measurement specimen thus retained in the feed line CL is ejected from the nozzle 5 6 toward the orifice 5 5. The red blood cell measurement specimen sprayed from the nozzle 56 is surrounded by the diluent in the previous step (5) and flows through the orifice 5 5 in a sheath flow (for about 10 seconds). Then close the valves S V1, S V 7. (7) When the sheath flows through the orifice 55, the control section 500 calculates the number of red blood cells (RBC), the number of platelets (PLT), the blood volume (HCT), and the blood volume (HCT) based on the impedance change between the electrodes 5 8 and 67. Other analysis items. Step s 1 5) (1) Open the valves SV 2 0, SV 1 6 and then add negative pressure to the waste liquid chamber WC by the negative pressure pump p, thereby removing the residual of the mixing chamber 70 and the detector 50. The liquid drain is placed in the waste liquid chamber WC. Then close the valves SV20 and SV16. (2) Open the valves S VI 4, SV1 3, operate the syringe pump SR2 to discharge, and use the dagger to give the diluent to the mixing chamber 70 and the detector 50. Then close the valves sv4, SV13 (3) and open the valves SV1, SV2 'and then apply negative pressure to the waste chamber WC by the negative pressure pump P1, whereby the diluent is passed from the mixing chamber 70 through the feed line CL Place the bubble into the calendar fluid chamber. Then, the valves SV1 and SV2 are closed. This completes the cleaning operation. The negative pressure of the waste liquid chamber wc depends on the pressure sensor. According to the negative pressure pump P1, the pressure is constantly maintained at 100 to 300 mm.

313933.ptd Page 72 544516 V. Description of the invention (62) Mercury, and preferably in the range of 150 to 200 mmHg. When the amount of waste liquid stored in the waste liquid chamber WC reaches a predetermined amount, this situation is detected by the float switch J 7 and the liquid discharge pump P 2 is driven to discharge the waste liquid into the waste liquid container B2. Input 厶 / Display 1 section Input / Display section 3 provides the input program information to the user, so that the user can perform input operation without error, display the input information, analysis progress and analysis result. The input / display section 3 will be described in detail later. The control section 5 0 0 displays various day-time displays on the liquid crystal display 3 a based on the display information and a predetermined program stored in R 0 M combined with the control section. These screens include a main day face containing a start button to start analysis, a mode selection day face containing a mode selection button, and a setting day face for selecting analysis items. In this specific embodiment, the start button 5 1 6 and the mode selection button 5 1 2, 5 1 4 are displayed on the main day surface at the same time (Figures 4 2 (a), 4 3, and 4 4). The display operation that is always performed by the blood analysis device in the analysis process will be described with reference to the flowcharts of 31 (a) and 31 (b). The input / display section 3 performs a display operation as described below. As shown in Figure 31 (a), after the power supply of the blood analysis device is turned off (step S1), after a predetermined preparation period (step S2), the main day screen displaying the message "Ready" is displayed on the input / Liquid crystal display 3 a of display section 3 (Fig. 4 2 (a)). The mode selection buttons 5 1 2, 5 1 4 and the start button 5 1 8 are displayed on the main day. The user sets a sample container containing a sample in the sample setting section 6 as described above (step S4). The user then selects between whole blood mode and pre-diluted mode.

313933.ptd Page 73 544516 V. Description of the invention (63)-An analysis is performed (step S5). In particular, use one of the ^ 'selector buttons 512, 51 4 to make a selection. The second mode of the touch master is selected according to the set sample container), then the user touches and enters the second king's blood sample (for routine points), and only runs the segment seven to go to the whole blood mode button 5 1 2 , And if the sample is too dark, the red confusion concealer is shown;%-廿 々 is a pre-diluted sample, then # 田土 when the user touches the full mode (step ...) turns red. When the user touches the The activated button 5 1 6 is yellow after dilution. At this time, when the control section 500 activates the "type" button 514, the sister 516 is turned to 5 0 0d to change the color of the button. The motion color section 5〇c And the color-change control section 鲂 力 力 力, 动 纽 516, and at the same time based on the ^ y 0 pi weight type displayed on the main day surface (step S6). The control section 5 0 0 determines the whole blood pull type and the pre-dilution mode π 4 ”μ hit, which of the eight is selected, starts the programming process corresponding to the selected core (step s7). When based on the start button 5 1 6 given to people + eight 丄 + π Q々day s-丄When the input analysis operation is started, the display of input / display segment 3 is not switched from the main writing 1 v, Λ L. — Qu (di 42 (a)) to measurement (Picture 4 2 (b)). At this point, Fen Yan Ding said..% M1 The staff member ’s eyes are not on the measurement screen, but the analysis results are not displayed because the analysis has not yet been completed. It is presumed that Luo Yue γ ❿ 疋 丁 疋 疋 疋 is to take five important analysis items (WBC, RBC, HGB, HCT, PLT). / Tian Jin 疋 King blood private type 'red blood cell measurement specimen and white blood cell measurement test system from whole blood Sample preparation (steps S8, s9). When the pre-dilution mode is selected, the red blood cell measurement specimen and the white blood cell detection specimen grandchild are prepared from the whole blood sample (steps S16, si7).

Seoul

313933.ptd Page 74 544516 V. Description of the invention (64) The white blood cell measurement specimen prepared in step S 9 is used to first measure the white blood cells and heme (heme concentration) (step S 1 0). When the measurement results are obtained, the measurement result data of these two items are displayed on the current measurement screen (Fig. 42 (0)) (step 311). Using the red blood cell measurement specimen prepared in step S8, the red blood cell measurement is performed (step S12) and calculated PLT (number of platelets), HCT (blood volume), and other analysis items. Then the measurement and calculation results are displayed on the measurement screen (step S 1 4). At this time, the measurement results of the five items are displayed in a larger font on the measurement screen (page Figure 42 (d)). Then start the clearing operation to prepare for the next round of analysis (step S 1 5). Figure 3 1 (b) is a detailed flowchart of step S 1 5. Refer to Figure 3 1 (b) Check whether the touch panel is operated to display eight analysis items during the cleaning operation (step S 1 8). If eight display operations are performed, the display switches to eight display screens, of which eight analysis items are compared with Small self-display (Figure 42 (e)) (step S19). The eight-item display screens (Figures 4 2 (e) and 4 9) remain displayed until you touch the back button ("top" button) 5 3 4 ( Step S 2 0). When the back button 5 3 4 is touched The display switches back to the main screen (Fig. 42 (a)) (step S21). If no operation is performed in step S1, the five-item display day (Fig. 4 2 (d)) remains displayed until the cleaning operation is completed (Step S 2 2). When the cleaning operation is completed, the display switches to the main day surface (Figure 42 (a)) (Step S 2 1), and the routine returns to step S 3 and is ready to perform a sample analysis. If the eight display operations are not performed before the cleaning operation is completed, it is determined that no eight display operations are required, and the display screen automatically switches to the main day surface (Figure 42 (a)).

313933.ptd Page 75 544516 V. Description of the invention (65) It is determined whether to automatically switch to the main screen (Figure 4 2 (a)), and the control section 5 0 0 must determine whether the cleaning operation is completed. In this specific embodiment, the control section 500 determines that the cleaning operation is completed based on a signal applied to the control section 500 from a sensor provided in the analysis device. In order to achieve this, an additional timer can be set. The control section 5 0 0 is suitable for determining the completion of the cleaning operation based on the beginning of the cleaning operation for a predetermined period of time, and the display screen consists of five display screens (Figure 42 (d) ) Automatically switches to the main day (Figure 42 (a)). In addition, the control section 5 0 0 is suitable for deciding to automatically switch the display screen from the five display screens (picture 4 2 (d)) to the main day view (picture 42 (a)) after a predetermined time has elapsed after the cleaning operation is completed ). Next, we will explain the main screen and the measurement of daytime content. Figures 43 and 44 are sketches illustrating the contents of the main day. In particular, Figure 43 shows the day and night face when the whole blood mode is selected, and Figure 4 and 4 show the day and night face when the pre-diluted mode is selected. As shown, the whole blood mode button 5 1 2 and the pre-diluted mode button 5 1 4 are displayed in the analysis mode area 5 11. The start button 5 1 6 is displayed in the start button area. In addition, the printer feeds P / F button 5 1 8. Selects the menu buttons 5 2 0 such as calibration and other items. 2 Controls the accuracy of the analysis device 5 2 2. Displays the previous analysis sample measurement results. The button 5 2 4, the closing button 5 2 6 (for closing the analysis device after the cleaning operation), and the delivery button 5 2 8 for performing the delivery operation in the pre-diluted mode are displayed in the input / display section. Since the touch panel 3 b is superposed on the liquid crystal display 3 a, each button function is performed by touching (or pressing) a corresponding part of the touch panel 3 b with a finger or the like. Work

313933.ptd Page 76 544516 V. Description of the invention (66) The execution of energy is executed according to the program originally stored in the control section 5 0 0. The sample ID is loaded into the area 5 1 0 by inputting the number of keyboards (not shown in the figure), and the keyboard can be displayed as a keyboard if required. The choice of analysis mode will be explained. The whole blood mode button 5 1 2 and the pre-diluted mode button 5 1 4 shown in Fig. 4 and 3 are colored red and yellow, respectively. The colors are different. 4 Ugly 5 1 2, 5 1 4 can be distinguished by color, so the possibility of pressing the wrong key can be eliminated. For example, when the whole blood mode button 5 1 2 is touched, the whole blood mode button 5 1 2 is displayed in a three-dimensional manner and has been pressed, and the pre-diluted mode button 5 1 4 is displayed in a three-dimensional manner and is deleted. On the other hand, when the pre-dilution mode button 5 1 4 is touched, the pre-dilution mode button 5 1 4 display is pressed, and the whole blood mode button 51 2 display is deleted. The mode buttons touched by the two-dimensional touch panel are displayed in three dimensions. The selected mode button can then be clearly distinguished from another mode button, thereby eliminating the possibility of wrong mode selection. At the same time, the message “Whole blood” or “Pre-diluted” is displayed under the button 5 1 2 of the whole blood mode and the button 5 1 4 of the pre-diluted mode, to avoid the wrong selection of the mode. In addition, the colors activated at 5 5 16 are changed according to the selected analysis mode. As mentioned above, in the specific embodiment, the whole blood mode button 5 12 and the pre-diluted mode button 5 1 4 are displayed in red and yellow, respectively. When the whole blood mode button 5 1 2 is pressed to select the whole blood mode, the start button 5 1 6 turns red. When the pre-dilution mode button 5 1 4 is pressed to select the pre-dilution mode, the start button 5 1 6 turns yellow. The whole blood mode button 5 1 2 can always be displayed in red, or only when the whole blood mode is selected. In the same way, the pre- and diluted mode buttons 5 1 4 can always be displayed in yellow, or

313933.ptd Page 77 544516 V. Description of the invention (67) Only displayed in yellow when the pre-dilution mode is selected. In either case, it is simply desired that the whole blood mode button 5 1 2 and the pre-diluted mode button 5 1 4 do not display the same color at the same time. The colors of the individual mode buttons are better selected so that they can be easily distinguished from each other. Analysis is started when the start button 5 1 β is touched on the main screen. However, if the analysis system is started before α > 絜 operation is completed or when the analysis unit lacks reagents or malfunctions, the analysis device may malfunction. Therefore, the control section 501 monitors the signals from the driver, the road section 501, etc., the shortage of test reagents, and the analysis device. Then the analysis preparation is completed, the control section 5 00 allows the display start button 516 'to prevent the display of the start button 5 1 6 if the analysis k is in an unmeasureable state before the preparation is completed or when the analysis device fails, such as Figure 4-5. This can prevent the user from accidentally pressing the start button 5 1 6, so it is easy to find that the analysis cannot be started. Control section 5 0 0 For example, based on the output of the panel open / close sensor j 1, the sample detection sensor J 2 and the like, monitor the completion information of the cleaning operation and analyze the failure information of the device. When the start button is not displayed, the display is blank, so the measurement status message can be displayed in a large font in the blank area. Epidemic day-to-day — — —'_ ^ The attack will explain how to display the day-to-day during the analysis. Figure 4-6 shows the measurement day and day displayed immediately after the start button 5 1 6 starts analysis. Fig. 4 7 Optional page shows the measurement picture displayed after obtaining the measurement result with the white blood cell measurement sample ® Fig. 4 8 shows the measurement result obtained using the obtained red blood cell measurement sample as the measurement screen displayed immediately after analysis. As shown in Figures 4 6 and 4 7, the analysis is performed by displaying a graphic (triangle) 5 3 〇 indication, there is a color change on the bottom line 0

313933.ptd Page 78 544516 V. Description of the invention (68) These measurements show five basic analysis items (WBC, RBC, PLT, HGB, and HCT) in large fonts on the day. ° Because the measurement results are not immediately after the analysis starts Obtained, so the analysis items (and their units) are simply displayed. About 20 seconds after the start of the analysis, the measurement using the white blood cell measurement specimen was completed. The white blood cell and heme data obtained in this measurement are shown in Figure 47. About 80 seconds after the white blood cell measurement was completed, the measurement was completed using red blood cell measurement specimens. The measurement results of the five analysis items are shown in Figure 4-8. When the arrow button (forward button) 5 3 2 in the lower right corner of Figure 48 is touched, the day-to-day display of the eight measurement results is shown in Figure 4-9, which contains MCV (average body volume) in addition to the aforementioned five analysis items. , MCH (mean soma hemoglobin), and MCHC (mean soma hemoglobin concentration). This screen shows each analysis item measured by the blood analyzer. As the number of analysis items increases, the analysis items are shown in smaller words. ^ When the front button 532 is touched again before the screen shown in Fig. 49, the statistical data of the measurement results related to the use of the white blood cell measurement specimen and the red blood cell measurement specimen are shown in Figs. 50 and 51. In addition, the display can be switched from the eight-item display screen β 49 or 42 (e) to the five-item day view (the "or 42 (d)" from the start of the display to the completion of the cleaning operation in about 20 seconds without any Round ^, change;

31 3933.ptd Page 79 544516 V. Description of the invention (69) Of course, if the forward button 5 3 2 is not operated during the display of the eight-item display screen, the display automatically switches to the main screen without having to look at Figure 4-9 data. The input / display section 3 is suitable for displaying five basic analysis items after the analysis of this embodiment is started. However, the input / display section 3 is suitable for preliminary registration and selection of the analysis items desired from the measurable analysis items, and is separated from the eight display days and displays the screen containing only the selected analysis items as necessary. Figure 52 shows the day view of the first measurement screen initially registered as WBC and RBC. In order to register the required analysis items, the daytime menu button 5 2 0 shown in Figure 4 3 or 44 is touched to activate the setting screen. The program is executed on the setting screen to select the required analysis items. In this program, the font size is pre-defined according to the number of selected analysis items (for example, a font size of 20 points is used to display up to two analyses, and a font size of 16 points is used to display up to five analyses). The font size is determined based on the number of items in the analysis. The detection circuit of the resistance-type detection screen is used in the detection circuit of the resistance-type detection section 503, which requires a booster circuit to boost the DC voltage (12 volts) output from the power supply section 10 to 50 volts or higher. In the present invention, a Cochlo power supply is used as the boost circuit. The detection circuit of the resistance detection section 503 will be described later. Fig. 53 is a basic circuit diagram of the detection circuit of the resistance detection section 503. As shown, the DC voltage is applied by a Cochlo power supply 830 to a series circuit consisting of a constant current circuit 840 and a resistive detection section 503. The terminal voltage of the resistance detection section 5 0 3 passes through the capacitor 8 5 2 into the amplifier circuit 8 5 5. The capacitor 8 5 2 can remove the DC component.

313933.ptd Page 80 544516 V. Description of the invention (70) The amplified voltage output is used as the detection signal. When a particle such as a blood cell passes through the orifice 5 5 (Fig. 23), the impedance between the electrodes 5 8 and 67 (refer to Figs. 23 and 24) fluctuates slightly. Because the constant current circuit 8 4 0 often applies a constant current to the orifice 5 5, a slight voltage change occurs between the electrodes 58 and 67 as the impedance fluctuates. After the DC component is removed by the capacitor 85 2, the voltage change is input to the amplifier 8 5 5 to obtain a detection signal. The attack will explain the Cochlo power supply 830. The Cochlo power supply 830 includes an oscillator 831, a switching circuit 832, and a booster 833, as shown in FIG. 54. The vibrating plate is 831 including an RC oscillator circuit. The R c oscillation circuit is made of an operational amplifier element 831a, a resistor R, and a capacitor c; it is connected to RC by 1 including an inverting operational amplifier element 8 3 1 b One of the branch output terminals of the oscillation circuit. With this configuration, the inverse 2 square wave A shown in Fig. 60 is output from the oscillator 831. The output supply of the square wave is switched 8-2. ^ The switching circuit 8 3 2 includes a second analog switching element 8 3 2a, 832 DC power supply (+1 2 volts), and a second analog switching 8 3 2c connected to a ground terminal. , # Analog switch elements 832a, 83 仏 are synchronous with square wave A. Switching elements 8 3 2 b, 8 32c are synchronous with square wave A, and the ^ phase can be opened and closed. And A has inverse analog switching elements 832a, 832, which are connected in pairs, pieces 8., 8 are connected in pairs. Analogy == 疋 卞 Pairs are transmitted through the output

544516 V. Description of the invention (71) Terminals T 1 and T 2 are connected to the booster 8 μ respectively. In the switching circuit 8 32, the four analog switching elements are switched in synchronization with the square wave A, A '. When a +12 volt voltage is applied to one of the wheels and the target T2, the other-output terminal is grounded and therefore has 0 volts, the terminals T1 and π are switched between +12 and 0 volts. Connected to the output terminals η, τ2 of the switching circuit 832. Boost crying: 833-connector C Yuc and diodes D to D to increase the voltage. The enclosing electrode is connected to the anode of diode D, and the diode D and the cathode Df anode. The other two-pole systems are connected to the two with this cathode system. The capacitor C is connected to the two adjacent diodes in series. However, the third capacitor C if is connected between the switching circuit 8 3 2 and the common cathode. Between polar bodies. d2 wheel output terminal η and two are alternately applied + 12 2 volts by the switching circuit 8 8 3 3 ^ ^ ^ MH ^ ^ ^; Λ = a capacitor and four diodes are connected to each other to obtain the output voltage 5 5 volts, jl jimu towers at 19, f, xi 仏 ~ ^ 戌 Shi Xun in the product of 12 volts times 5. The value calculated by π is 60 volts, but the forward voltage drop of the diode is actually reduced. The voltage after the shock is output through the rectifier diode D and the smoothing capacitor C to the subsequent circuit. … Often, a constant current circuit, such as a current mirror circuit using a transistor, can be used as the constant current circuit 840, and the circuit 84 0 is supplied with a voltage generated by the Cochlo power supply 8 3 0. Check whether the Kekelow power supply 83 is stable as a liter

313933.ptd

544516 V. Description of the invention (72) Voltage circuit. The experimental results are shown below. The basic structure of the experimental circuit is shown in Figure 5-5. As shown in Fig. 55, the Cochlo power supply 8 300 shown in Fig. 54 is connected to a constant current circuit 8 400, which is used for these applications. Crystal's current mirror circuit. The constant current circuit 8 4 0 includes an impedance coil L and a capacitor C 6 for smoothing filtering, a resistor (resistance R s) 8 4 2 which sets a current level, and a resistance detection section 5 0 3 An impedance dummy resistor 843, a Zener diode 8 44 which generates a reference voltage E s, a transistor Q 2 and a Q 2, resistors R 3 and R 4, a capacitor C 7 and an operation switch S. The resistance R s of the current setting resistor 8 4 2 is appropriately set as a parameter for determining a current level equal to E s / R s. The experimental data referring to the relationship between the switching frequency (kilohertz) and the output voltage (volts) of the Cochlo power supply 8 8 0 and the oscillator 8 3 1 are shown in Table 1 and Figure 5-7.

313933.ptd Page 83 544516 V. Description of the invention (73) Table 1 Switching frequency (KHz) No load current is 0.75 mA Amps Load current is 2.50 mA Amps Load current 1 58.1 47.5 31.5 10 58.0 53.6 46.7 50 58.0 53.8 47.2 1 〇〇58.0 53.8 47.1 200 58.0 53.7 47.1 500 57.9 53.6 46.7 1000 58.0 53.2 45.9 2000 58.2 52.3 43.5 3000 Γ 58.1 49.5 35.7 In the experiment, a pulse generator was used instead of the oscillation circuit 8 3 1 to conveniently change the switching frequency. Another micro-Faraday ceramic capacitor uses capacitors C to C 4 as boosters 8 3 3. By adjusting the current setting resistor 8 4 2 of the constant current circuit 8 4 1, a load current of 0, 0.75 and 2.5 mA is applied. At this time, the output voltage of the Cochlo power supply is measured at the terminal TP1 provided in the constant current circuit 841. As can be seen from Table 1 and Figures 5 and 7, the output voltage does not depend on the switching frequency and does not cause a load current to the constant current circuit. On the other hand, using a higher load current, it is better to increase the output voltage as the switching frequency increases. However, when the switching frequency is higher than a certain level, the output voltage drops. The reason is presumed that the output voltage is affected by the operating speed of the analog switching element 8 32 and the 0 N resistance. In addition, the output voltage drops at lower switching frequencies. Presumption

313933.ptd Page 84 544516 V. Description of the invention (74) The reason is that the lightning current supply at lower frequency should be lowered in posture # ^ The power supply should be insufficient to maintain the output voltage. Said to be extremely sticky / the original driver should be inserted at a switching frequency of 50 to 100 kilohertz, providing an output of about 50 volts, which is slightly more stringent, followed by 'instruction boosting / ^ ru W to The relationship between the good capacitance (micro-Faraday) and the output electric calendar (hot) is not shown in Table 2 and Figure 58. Table 2

Ci to C4 capacitors without load current '(micro-Faraday) 1 58.3 0.1 58.2-0.01 58.1 — with 0.75 mA current and 2.50 mA load current 44.2 53.7 50.9 47.8 47. 1 39.0 Only electric valley with electric valley 1, 0 · 1 and 0 · 0 丨 micro-Faraday ceramic capacitors are used as capacitors C to C4 of the booster 8 3 3. By adjusting the current setting resistor 8 2 2 of the constant current circuit 841, load currents of 0, 0.75, and 2-5 mA are applied as in the previous experiment. Then measure the output voltage of the Cochlo power supply at terminal Tρ 丨. As can be seen from Table 2 and Figures 5 and 8, the output voltage does not depend on the switching frequency of the no-load current. The current capacity of the power supply increases with the capacitor capacitance as the load current increases. Therefore, in terms of current capacity, a larger capacitance is advantageous, but a capacitor with a larger capacitance is larger. Therefore, the experimental data suggest that the capacitive state with a capacitance of 21 micro Faraday will not cause problems in practical applications. … Its attack ”The switching frequency of the Cochlo power supply and the circuit constant of the capacitor are based on the relationship between the switching frequency and the output voltage, and the capacitor

313933.ptd Page 85 544516 V. Description of the Invention (75) The relationship between the capacitor and the output voltage is adjusted to be optimal, and the performance of the power supply is checked. Experimental data indicating the relationship between load current and power supply voltage. The results are shown in Tables 3 and 5-9. The switching frequency is set to 160 kilohertz which falls within the aforementioned preferred range (the oscillating circuit of Fig. 54 produces 8 7 ohms, C f 1 0 0 is a micro-Faraday), and the capacitors C and C each There are capacitors of 1 micro Faraday. Table 3 Load current (milliamps) DC composition of voltage at TP1 (volts) AC composition (mVrms) 0.09 56.4 0.30 0.25 55.8 0.31 0.53 54.7 0.33 0.76 53.8 0.36 0.92 53.2 0.37 1.64 50.4 0.47 2.46 47.4 0.60 6.74 28.7 1.53 Figures 3 and 5-9 show that as the load current increases, the DC component decreases and the AC component increases. When the load current is not less than 1.6 mA, the DC component drops to 50 volts or less. Fully meet the needs of power supply for detection circuit (output voltage is not less than 50 volts, load current is 0 · 6 to 1 mA). Therefore, experimental data indicate that it is better to use a Cochlo power supply as a boost circuit. Second, measure the power consumption of Cochlo power supplies. The measurement results show

313933.ptd Page 86 M4516 V. Description of Invention (76) is shown below. " ~ Measurement of power consumption Change the component and circuit connected to t; t switch the resistor (about 10 ohms) to a lower level and observe the voltage drop. DC input voltage source (+ 12 volts) As a result, ready to wait for the sundial ^ Switching operation of the source supplier II: The power consumption is 3 milliwatts (at this time Cochlo electric watts (with a load current of 0.75 milliamps) Talk ^ § When the detection current is ON, it is 83 millimeters. When it is ready, it is ready to wait: Show the ancient DC-DC converters, current-DC converters, electricity, 5 8 0 < c watts (5 volt voltage supply direct ON When the power consumption is 6 ^ not ^ load) 'and when the detection current is pei). ⑼ Mao watt (possible load current = 0.75 mA using Cochlo power supply is lower than using a commercially available DC_Jiffa The power consumption of the road is far away, and it is relatively familiar: analysis of straight lines: power transfer; step-down circuit of the booster ^ ^ reduction.… The result of the power consumption of the booster circuit used, using the branch克洛 power supply production wow evening 埶 department # / π VW tail circuit during operation ^… π1, so it can be automatically cooled without the need to borrow cooling fan tower cooling system booster circuit. PF fan 4 strong tf jj a ϋ_ϋ 一 ^ After the holder and the flow path n mechanism m, the container housing unit shown in FIG. 3 will be held. 0 valley device holder 95 0 (Figures 6 and 9) and the flow path connection for fluid communication between the container housing and the main body of the analysis device ^ 70 Dust mi standing is called Zhu Ming.

3] 3933.ptd

544516 V. Description of the invention (77) As shown in Fig. 61, the container housing unit 1 0 0 includes an inner container 9 0 1, a large container approximately 9 0 2, 9 0 3, and a small container approximately 4 Container 9 0 4. As shown in Fig. 61, the inner cymbal 9 01 is rectangular in shape, and has an open top and a handle hole 9 06 formed on the side wall, which is convenient for the user to insert the finger into the hole to control the inner cymbal 9 0 1. The formation of the handle hole 9 06 can be achieved by the inner part of the drilled part and tearing away from the drilled part. The internal configuration and dimensions of the inner container 9 0 1 are defined according to the external placement and dimensions of the two large containers 9 0 2 and 9 0 3. The second two containers 9 0 2 and 9 0 3 are arranged adjacently, and the container 9 02, The opposite faces of 9 0 (indicated by the reference letter S in Figure 61) are in contact with each other, so the two large containers 9 0 2 and 9 0 3 can be cleanly accommodated and placed inside the inner 9 1. If due to the positional relationship between the large container and the guide mechanism 9 7 0 attached to the container holder 9 50, the large container needs to be arranged in an adjacent relationship separated from each other, the internal configuration of the inner tube 9 0 1 The external configuration and external dimensions of all the containers that are housed in the inner container 9101. Therefore, the container can be properly positioned inside the inside 901. The inner shell is made of, for example, a rigid board or plastic. The inner pan has a lid and a container is contained therein. One of the large containers 9 0 2 and 9 0 3 is used as a diluent container B, and the other large container is used as a waste liquid container B 2, as shown in FIG. 29. The configuration of individual containers will be described next. Figure 62 shows the structure of a large container, and Figure 63 shows the structure of a small container. Such containers are manufactured, for example, by blow molding from plastics such as HDPE (High Density Polyethylene). The large containers 9 0 2 and 9 0 3 each have a roughly rectangular groove portion (container body) 9 1 0 for containing reagents, and each has a λ !, and a diameter mouth P 9 1 1 is provided on the upper part of the container body 9 1 0 It is used to facilitate the entry and exit of the reagent from the container body. Mouth 9 1 1 is provided with a thread 9 1 2

313933.pid Page 88 544516 V. Description of Invention (78) Edge. When the large container 9 0 2 or 9 0 3 is to be sealed, the outer cover (not shown) is nested in the mouth 9 by screwing. The large container 9 0 2 and 9 0 3 are in use. 'The inner cover 9 1 3 is intended to close the mouth 9 1 1 俾. The container body need not be rectangular, but may be cylindrical. The large containers 902 and 90 3 of FIG. 62 have a shoulder convex portion 92 3 provided on the shoulder thereof. The shoulder protrusion 9 2 3 prevents the container housing unit 100 from being set in the wrong direction of the container holder 9 50. The container holder "o" of Fig. 69 is provided with three guide mechanisms 9 7 0. The containers 9 0 2, 9 0 3, 9 0 4 are used exclusively for waste liquid, hemolysing agent and diluent. Therefore, the container 9 〇2, 9 0 3, 9 0 4 must be set to the container holder 9 50, and properly connected to the guide mechanism 9 7 〇 for individual use. If the household convex 9 2 3 ', the container housing unit 1 〇〇 may be set in the wrong direction, seabass results in the wrong communication between the waste liquid and the diluent. However, if the internal 箧 丨 〇〇 (Figure 6) ... Shoulder convex portion 9 2 3 is oriented in the wrong direction] If the container holder 950 is set in the wrong direction, the shoulder convex portion 92 3 of the large container μ, 冋 ΘΟ 3 faces away from the container holder 95 and the wall convex portion 955 ( It is the same height as the shoulder convex part 9 23 of the large container 9 0 2 and 903 (as shown in the convex diagram), so the container housing unit 100 cannot be set in the container clip. Brother 69 9 50. This is possible Prevent the container housing unit 100 from being set in the wrong direction of the container 950. Figure 64 is a cross-sectional view of the large container 902 and 903 with the inner cover 91 3 capped. The inner cover 9 1 3 has Flexible and resistant to the reagents used; the inner cover 91 3 is, for example, made of silicone rubber. The inner cover 913 has a @ 'hole 914 and a ventilation hole 915. The ventilation hole 915 allows air to communicate. The straight channel stops the pressure of the container from rising and falling when the reagent passes through the fluid channel hole 9 1 4.

544516 V. Description of the invention (79) The flow path pipe 9 1 6 which is the flow path for connecting the fluid passage hole 9 1 4 is suspended inside the large container 9 0 2, 9 0 3 (the container body 9 1 0). The distal end of the flow path tube 9 1 6 reaches the bottom of the container, so even if a small amount of reagent remains, the reagent can still be sucked out through the flow path tube 9 1 6. The tube includes, for example, an amino tube, a polysiloxane tube, and a tetrafluoroethylene tube. The shoulder 91 7 extends in the radial direction outward from the bottom of the mouth 9 1 1, and then the container side wall 9 1 8. The container body 9 10 is delimited by the internal space of the container side wall 9 1 8. The shoulder portion 9 1 7 has a neck portion 9 1 9 which is placed over the mouth portion 9 11. When the small volume is 9 0 4 resting on the shoulder 9 1 7, the neck 9 1 9 is used to fix the small container 9 0 4 Tian Bilin facing away from the small container 9 0 4 side wall. 6 7 ^ ° $ 员 部 9 1 9 The diameter (external dimension) is measured according to the outer diameter (external dimension) of the small container 9 〇4. The container convex portion 9 2 2 (detailed later) is set at the mouth of the large container 9 1 1 side wall. The small container 9 shown in Figure 63 is a container side wall 9 3 9 which defines a groove (container body 931) for storing reagents and a small straight A f 4 9 3 2 It is arranged on the container body 9 3 丨. The mouth portion 9 3 2 is used to facilitate the entrance and exit of the reagent. As can be seen from the sectional view of FIG. 65, the small container 9 〇4 has a slightly flat bottom surface 9 4 2 The bottom surface is not required to have a flatness, but the bottom surface configuration is determined by the positional relationship between the shoulders of the large container 902, 903 and the small container installation portion 9 24 If the large capacity crying, the small container mounting portion 9 2 4 of the shoulder restraint is slightly inclined, then the bottom surface of the small valley state 9 0 4 q: »: > f ^. — Y 4 2 is configured to be equal to the inclination . Therefore, the bottom surface may be roughly flat. © 久 可能 & w & μ Putting a wave, bending or tilting. The small container mounting portion 9 ZZ 4 is not only a positive flat configuration, but also allows the small container 9 0 4

313933 · pul Page 90 544516 V. Description of the invention (80) It is placed between the two large juxtaposed containers 902 and 903. Therefore, the configuration of the bottom surface 9 4 2 of the small container 9 04 is not limited to a rough planar configuration, but may be equal to the configuration configuration of the small container mounting portion 9 2 4. The small container 9 0 4 is used as the hemolytic agent container B 3 shown in Fig. 29. The thread 933 is provided around the mouth 9 3 2, the inner cover 9 34, the fluid passage hole 9 3 5, the ventilation hole 9 3 6, the shoulder 9 3 7, the flow path tube 9 38 and the container convex portion 941. The thread 9 33 is substantially It has the same structure and function as the large container 9 0 2 and 9 0 3, so there is no need to explain here. The side wall 9 3 9 of the small container 9 0 4 is roughly disc-shaped, and its diameter is defined to be in contact with the neck 919 of the large container 902 and 90 3 (refer to FIG. 61). The neck 9 1 9 and the container side wall 9 3 9 of the young woman 9 0 4 are shown in a circular configuration in the drawings, but the configuration of the neck 9 1 9 and the container side wall 9 3 9 is not limited to a circular shape. For example, the neck 9 1 9 and the container side wall 9 3 9 may be polygonal, and its dimensions are such that the neck 9 1 9 and the container side wall 9 3 9 are in contact with each other. As mentioned above, the shoulders 9 1 7 protruding from the large container 9 0 2, 9 0 3 neck 9 9 9 in the radial direction are arranged in a roughly flat plane, and the bottom surface of the small container 9 0 4 has a shape equal to the shoulder. The schematic plan layout of the section 9 1 7. Therefore, the small container 904 can be easily mounted on the shoulder 917 of the large containers 902 and 90 3. The convex portion 9 2 0 is individually provided in the large container 9 0 2, 9 0 3 and the neck portion 9 1 9 will be in contact with the small valley 9 0 4. On the other hand, the small container 904 is formed with a recessed portion 9 40, and the recessed portion 9 40 can be combined with the protruding portion 9 2 0. Therefore, the small container 9 0 4 is clamped between the neck 9 1 9 and the concave portion 9 4 0 is a coupling convex portion 9 2 0. Therefore, it is ensured that it is firmly fixed in the large container 9 0 2 and 9 0 3 without appearing. Lateral displacement.

31 3933.pid Page 91 544516 V. Description of the invention (81) In addition, the annular convex portion may be provided on the shoulder portion 9 1 7 and the outline of the small container 9 0 4, so as to limit the lateral displacement. The flange 9 2 1 is provided on the upper part of the convex part 9 2 0 of the neck 9 1 9 of the large container 9 0 2, 9 0 3. The flange 9 2 1 individually overlaps the small container 904 portion, which not only prevents lateral displacement of the small container 904, but also prevents vertical displacement. Next, it will be explained how to accommodate the large container 90, 903 and the small container 904 in the inner container 901. The two large containers 9 0 2 and 9 0 3 are arranged in an adjacent relationship. Therefore, when the large containers 9 0 2 and 9 0 3 are accommodated in the inner container 9 0 1, the large containers 9 0 2 and 9 0 3 are intended to contact each other. Surfaces (contact surfaces indicated by reference symbol S in Figure 61) are spaced a small distance (about 1 cm). The small container 9 0 4 rests on the middle flat area between the shoulders 9 1 7 of the two large containers 9 0 2 and 9 0 3. Then the two large containers 9 0 2 and 9 0 3 are carefully moved towards each other and adjusted to contact each other. Therefore, the convex part of the neck 9 2 2 9 0 3 is embedded in the small container 9 0 4 Concave portion 9 4 0 of the container side wall 9 3 9. Fig. 66 is a perspective view showing a state in which the surfaces S of the two large containers 9 0 2 and 9 0 3 are in contact with each other. Figures 6 7 and 68 are the front view and the plan view showing this state, respectively. The surfaces of the two large containers 9 0 2 and 9 03 are in contact with each other, and the small container 9 0 4 is fixed by the neck 9 1 9, the convex portion 9 2 0 and the flange 9 2 1. In this state, the two large containers 9 0 2 and 9 0 3 are raised and inserted into the inner cymbal 901. The inner container 901 and the two large containers 902 and 90 3 have appropriate dimensions, so the large container 9 0 2, 9 0 3 is embedded in the inner container 9 (H. Therefore, the large container 9 0 2, 9 0 3 and the small container 9 0 4 Simply by inserting the large container 9 0 2, 9 0 3 into the inner tube 901,

313933.ptd Page 92 544516 V. Description of the Invention (82) Positioning. In this way, the large container 9 0 2, 9 0 3 and the small container 9 0 4 are accommodated in the inner container 9 0 1, and the small container 9 0 4 is installed on the shoulder 9 1 7 of the large container 9 0 2, 9 0 3 . The container holder I _ will describe a container holder attached to the analysis device body 1 for holding the container housing unit 100. The appearance of the container holder (including the container housing unit 100) is shown in FIG. 61, and the container holder (excluding the container housing unit 100) is shown in FIG. 6-9. The container housing unit 100 is housed in a container holder 950. In use, the container holder 9 50 is fixed to the blood analysis device body 1 by fixing screws 9 5 6 and 9 5 7 as shown in FIG. 3. The container holder 950 is a roughly rectangular box shape having six sides. Of the six faces, an acute angle is cut out from the upper right portion of a front side wall 9 51, and a bottom wall 9 52, a rear wall 9 5 3 and a left wall 9 5 4 are opened on the top and right sides. The internal dimensions of the container holder 9 50 are equal to the internal dimensions of the container housing unit 100 0 and the external dimensions of 9 0 1. The inside of the container housing unit 1 0 0 9 1 is inserted into the container holder 9 50 0 from the right side, adjacent to the wall 9 5 4 facing away from the left side. In this way, the 9 0 1 is properly positioned on the container holder 9 5 0. Therefore, the large container 9 0 2 and 9 0 3 embedded in the inner crotch 9 0 1 and the small container 9 0 2 and the small container 9 0 4 9 9 fixed to the large container 9 0 4 The guide mechanism attached to the container holder 9 50 is adjusted to a predetermined positional relationship. The inner handle L 9 0 6 is exposed from the cut-out portion of the front side 9 51, so the user can easily insert his finger into the handle hole 9 0 6 and drag the container housing unit 100. Interference connection mechanism Next, the fluid used between the container and the blood analysis device body 1 will be described.

313933.pid Page 93 544516 V. Description of the invention (83) The connecting flow path connecting mechanism, the connecting mechanism doubles the reagent supply analysis device body, and the future; from the reagent container The waste liquid is drained into the waste liquid container. Each of the flow path connecting mechanisms of the self-extinguishing device body 1 is composed of constituent elements provided on the outside of the container. The settings are described in reference to Figures 64 and 65. In particular, the 64th ^ = component has 9! 4, the inner cover with a vent hole 9! 5 consists of the wide fluid passage hole π, Λ dish y 1 micro by the inner 盍 9 1 3, and ancient The total number of cigarettes 9 1 6; or the fluid passage holes 9 3 5 & 934 shown in Fig. 65 and the flow path pipe 9 suspended by the inner cover 934 has an inner cover through the air hole 936-part. ^ & The first part of the flow path connection mechanism: the components of the flow path connection mechanism outside the container are shown in the perspective view of Figure 61 and Figure 6g, and are used for the following description. The flow path is connected to Ur 2: The diagram and other diagrams adopt 97〇. The reverse connection mechanisms each include a nozzle 960 and a guide mechanism guide mechanism 97. Attached to the container holder 9500 wall 9 5 3 = port 98 two. Three flow path connection mechanisms are provided corresponding to the three shown in the drawings. Gu Yi. However, since the structure of the flow path connection mechanism is the same, only one of the flow path connection mechanisms will be described. Valley m soil Figures 70 to 73 show the structure of the two-path connection mechanism guide mechanism 97 as seen on the right side (arrow direction A) of Figures 61 and 69. The figures will also be used to illustrate the operation of the guide mechanism 9 70. The seventh marriage is (see when the guide mechanism has the position shown in the figure (detailed later)) the center cross-sectional view of the guide mechanism 97. First, the nozzle 960 will be described. The nozzle 96 is provided at the guide The inside of the guide mechanism 97 ° (Figure 61). The nozzle 9 6 0 is roughly cylindrical with a flow path provided therein.

544516 V. Invented the Ί 儿 明 (84) liquid nozzle 9 6 1 as shown in the sectional view of FIG. 7 4. One end of the flow path 9 6 1 is inserted into the channel hole 9 1 4 (Fig. 64), and the other end of the flow path 9 6 1 is connected through a pipe to 'suspend' the port of the analysis device body 1. If you want to insert the fluid channel hole to the tip, the tip is tapered to easily insert the fluid channel hole 9 1 4. < The flow path 9 6 1 of the nozzle 9 6 0 is bent at a right angle at the center of the nozzle, and is even provided at the port 9 6 4 in the axial center of the cylindrical side wall of the nozzle 9 60. System connection The analysis device body 1 is connected to the port 9 6 4 as a fluid communication pipe system. + $ 如 液 A support hole 9 6 3 is provided at the tail. The second shaft 9 73 (see below for more details ^ mouth 96 〇 runs through the support hole 9 6 3, so the nozzle 9 6 0 is supported by the second shaft 9 7 3 = fan) extension hole 9 6 3 is provided at the tail of the nozzle 9 6 0 Therefore, the 9 6 mouth of the nozzle tip supports vertically downward. 9 Xia Li guide bow 丨 Referring to Figures 61 to 70, the guide mechanism 97 will be a mechanism 97 0 including a first lever, the first lever is fixed = guide bow 丨 container holder 9 5 0 wall 9 The supporting element f of the notch 988 of 5 3 is formed at: ... the zone-turning support at one end thereof; a second lever m ,: shaft) Chuan Weizheyudi—the second supporting element at the other end of the lever 972 Second, the pivotal support is from the Fuxin; and the third lever 975, which is connected with the current) 9 73 is in the middle 9 72 is supported at the one end with the -axis 971 as the center. Supporting the same brother one "tribute stick 70 pieces of material is preferably SUS303. The second ABS resin. 』? For example, as shown in Figs. 61 and 69, the first dried lever 9 is preferably the first lever 9 72, and the lever 9 75 surrounds the nozzle 960 974 and the cover 9 1 3. u See inside the container. The second lever 9 7 5 is located inward of the first lever 9 72. Second loss

544516 V. Description of the invention (85) The lever 9 74 is located inward of the first lever 9 72. The third lever 9 7 5 has a step inside disposed at the distal end thereof, and does not interfere with the second lever 9 7 4. Mai according to Figures 6, 9, 70 and 74, which will further explain the details of the first lever 9 7 2 as follows. The first lever 9 7 2 has an elongated through hole 9 8 7, and the first shaft 971 extends through the through hole 9 8 7. The through-hole 9 87 allows the first lever 9 72 to move slightly in its longitudinal direction. The first lever 9 7 2 has a stopper 98 9 which is disposed at a small distance between the elongated through holes 9 8 7 in the transverse direction. The stop 9 8 9 is adjusted to be adjacent to the back of the wall 9 5 3. When the flow path connecting mechanism is raised as shown in FIG. 70 (or FIG. 61), it is used to prevent the wireless connection of the first lever 9 7 2 Pivot movement. The second lever 9 7 5 is supported by the first shaft 9 7 1 in the direction of the first lever 9 7 2 = inward. The third lever 9 7 5 has a convex portion 9 7 8 and the first lever 9 7 2 has a slender perforated hole 9 7 9. The convex portion 9 7 8 is nested in the through hole 9 7 9 to prevent the third lever. 9 75 moves unlimitedly away from the first lever 9 7 2. Therefore, the third 1-belt 9 7 5 is allowed to move relative to the first lever 9 7 2 within a certain angle range, so that the convex portion 9 78 can be moved inside the elongated through-hole 9 7 9. The middle portion 9 9 0 (Fig. 69) extending in the width direction between the right portion and the left portion of the first lever 9 7 2 has a flat shape. With reference to Figures 6, 9, 70 and 74, the second lever 9 74 will explain further details as follows. The second lever 9 74 is supported by a second shaft 973. The second lever 9 7 4 has a cup shape and has an inner space 9 8 0 (7 4 f) for receiving the inner cover 9 1 3 therein. The second shaft 9 7 3 supports the nozzle 9 60 to fix the internal space 98 inside the t. The inner cover 913 is inserted into the inner space 98 °, and the nozzle tip 9 6 2 is inserted into the fluid passage hole 9 丨 4 (refer to FIG. 6 丨).

313933.Ptd Page 96 544516

V. Description of the invention (86) As shown in FIG. 70, the first; lever 974 has a cloud-shaped recess 98 2¾ placed on its inner surface, and the recess 9 8 2 will be adjusted so that it is not placed on the peripheral surface of the container 9 1 1 The container protrusion 9 2 2. The concave portion 9 8 2 has a cloud-shaped back and a curved side drawing. Therefore, when the second rod 9 7 4 pivots about the second shaft 9 7 3 as the center, the container convex portion 9 2 2 enters the concave portion 982 and faces the concave portion 98 2 Enter less forward. In this way, the second rod 椁 g74 can be fixed to the large container 902 by coupling the container convex portion 92 2. The second lever 9 7 4 has a lever 9 8 3 to facilitate the pivoting operation of the second lever 9 7 4. Referring to Figures 6, 9, 70 and 74, the details of the third lever 9 75 are further explained, as described later. The third lever 9 7 5 has a pressing portion 9 8 4 (refer to Figures 6 9 and 61 1) ^ placed on the other end to press the inner cover 9 1 3 toward the inner cover 9 1 3 next to it; Part 98 5 (refer to Figures 69 and 70), the first lever 9 7 2 is raised and the second lever 9 7 4 is supported. Μ is arranged in accordance with the dust part 9 8 4 series so that the convex part protrudes inward and is located under pressure The inner cover 913 of the second lever 9 7 5 in the inner direction (refer to Fig. 61). On the contrary, the second part 9 8 5 protrudes outward to support the second lever MU. 9 7 2 and the third lever 9 7 5 翱 Α π # When moving to the right inside the elongated through hole 979), ς: f and move (the contact position between the convex MS rod 974 gradually changes / supports the convex. H85 and the first The second lever's contact position supports the second lever. ^ Supports the convex. 卩 9 8 5 is the second lever 9 7 4 of the changing 9 8 5 I 2nd and 2nd faces: To adjust to this adjacent support convex part is set as the contact position At the time of change, ^ _-卩 is adjacent to the surface R (refer to Figure 7 4) is connected to the third lever 9 7 5 right H rod ^ Ho counterclockwise pivot. Face shape. In addition, Zhongqiu 991 right, Gongzhizhi The middle part 991 (Figures 6 and 9) is flat. &Quot; 91 Arcuate cutout 992, so that when the third:

544516 V. Inventor (87) When the lever 9 7 5 is lowered (refer to Figure 6 9), it will not interfere with the mouth 9 11 of the container, and avoid collision with the mouth 9 Π. The torsion spring 9 7 6 is nested in the first shaft 9 71 to apply a biasing force to the first lever 9 72 relative to the rear wall 9 5 3 to maintain the guide mechanism 9 7 0 in a lifted state and the nozzle 9 6 0 The fluid passage hole 9 1 4 is not inserted (in the state shown in Fig. 61). The torsion spring 9 7 7 is nested in the first shaft 9 7 1 to apply a biasing force to the first lever 9 7 2 and the third lever 9 7 5 away from each other, and the guide mechanism 9 7 0 is maintained in a lifted state ( Figures 75 to 77). The two torsion springs are configured so that they do not interfere with each other. In other words, the torsion spring 9 7 6 has right and left coil portions wound around the divided portion of the first shaft 9 7 1 bis, and a middle portion A extends axially outward from the coil to be wound, as shown in FIG. 7 5 . The torsion spring 9 7 7 has a generally straight coil shape, and is located inside the bypassed middle portion A of the torsion spring 9 7 6. As shown in Figures 76 and 77, the middle portion A of the torsion spring 9 7 6 is supported adjacent to the first lever 9 7 2 middle portion 9 9 0, and the end portion B of the torsion spring 9 7 6 is fixed to the wall 9 5 3 (picture 6-9) of the coil fixing part 9 9 3 (picture 7 5). The ends of the torsion spring 9 7 7 are respectively supported by D to be adjacent to facing away from the first yoke 9 7 2 middle 9 9 0 and the third lever 9 7 5 middle 9 9 b. The attack will explain the guide mechanism. As shown in Figures 70 to 73, the guide mechanism 97 is gradually pivoted from the free position (raised position), and the nozzle g is inserted.

544516

And the stop block 98 9 remains stationary adjacent to the back of the wall 9 5 3 V. Description of the invention (88) The biasing force is deflected away (Figure 69). At this time, the third lever 9 75 is deflected away from the first lever 9? 2 by the biasing force of the nested first shaft 971 9 77 (Fig. 75).

9 78 remains stationary adjacent the end of the elongated through-hole 9 79. Convex J The second lever 9 7 4 is supported by the second shaft 9 7 3 as the center. It is supported by the convex portion 9 8 5 and partly adjacent to the first convex portion 9 8 5 by gravity guidance. Further under the condition of supporting three levers 9 7 5, the user holds the lever of the second cross bar 9 74 _ when the second lever 974 is pivoted in the direction X of the hoe, the supporting protrusion 985 maintains the second lever 9 74—For a period of time, let the first lever 9 72, the second lever, 974 and the third lever 9 9 5 pivot together. 2. The figure of the younger brother 71 showed that the second crossbar 97 5 was dragged into a roughly horizontal orientation and contacted the inner cover 9 1 3. When the third lever 9 7 5 is adjusted to contact the inner cover ^ 9 i 3, the inner cover 9 1 3 is pressed down by the pressing portion 9 8 4. The inner cover 9 1 3 prevents further rotation of the " triple bar 9 7 5 ". Therefore, when a force is applied to the lever 9 83 of the second lever 9 74, the first lever 972 starts to pivot against the biasing force of the torsion spring 9 7 7 acting on the first lever 9 7 2 and the third lever 9 7 5. In other words, the angle between the first lever 9 7 2 and the third lever g 75 is reduced, and the first lever 9 7 2 overlaps the third lever 9 75. Finally the first cross bar 9 7 2 is pivoted adjacent to the third lever 9 7 5 and thus becomes horizontally oriented. When the angle between the first lever 9 72 and the third lever 9 75 is reduced, the distance between the support protrusion 9 8 5 and the second shaft 9 7 3 is reduced, and the contact between the second lever 9 7 4 and the support protrusion 9 8 5 The position is changed along the adjacent surface R of the supporting protrusion shown in FIG. 74.

3Π933 · _ page 99 544516 V. Description of the invention (89) In this way, when the contact position is changed, the second lever 9 74 is allowed to pivot around the second axis 9 7 3. In the state shown in FIG. 71, the first lever 9 7 2 is centered on the first axis 971 and the second rotation lever 9 7 4 is pivoted on the second axis 9 7 3. Shortly after the reduction of the angle between the first lever 9 7 2 and the third lever 9 7 5 began, the nozzle tip 9 6 2 (Figure 7 4) was adjusted to contact the inner cover 9 1 3. Since the nozzle tip 9 6 2 is tapered, the nozzle 9 60 will be inserted into the fluid passage hole 9 1 4 by the guide of the tapered nozzle tip (Fig. 64). Since the first lever 9 7 2 is supported around the first shaft 9 71 extending through the elongated through hole 9 8 7, the first reed lever 9 72 has a freedom of movement in the longitudinal direction (longitudinal clearance). This degree of freedom allows the nozzle 96 to be smoothly inserted into the fluid & holes 9 14. Figure 72 shows that the first lever 9 72 is pivoted into a state of being roughly horizontally oriented adjacent to ^^ trib ^ 5. In this state, the nozzle 960 is completely inserted into the fluid through hole 9 1 4. Leaving the first lever 97 2 Tian adjacent to the third lever 9 75 'prevents the first lever 972 from being further pivoted. The support protrusion 985 and the second shaft 973 are positioned in the closest relationship to each other. The second lever 9 74 is closed by the position adjacent to the support protrusion 9 8 5 and then only the second lever 9 74 is allowed to be centered on the second 9 7 3 Pivot so that the container projection 9 2 2 enters the second lever 9 74 and the cloud-shaped recess 9 82. Fig. 73 illustrates the final state in which the container convex portion 922 is fixed to the cloud-like concave portion 98 2 of the second lever 97i. The second lever 9 74 is further rotated around the second axis 9 73 and then the container convex portion 9 2 2 is adjusted to the innermost part of the base adjacent cloud concave portion 982 and

313933.ptd 544516 5. The description of the invention (90) is set to this state (Figure 74). The nozzle 9 6 0 is inserted into the fluid passage hole 914 through the guide mechanism 9 7 0, and the flow path tube 9 1 6 (Figure 6 4) and the inner cover 9 1 3 are inserted into the fluid passage hole 914. The forcing hole 9 1 4 and the nozzle 9 6 0 are connected to each other, thereby establishing a flow path to the blood analysis device body 1. ^ As in this specific embodiment, it is desirable to provide a twist spring 9 7 7 for biasing, 1 for the first lever 9 7 2 and the third lever 9 7 5 which are separated from each other. However, because the second crossbar 9 75 is taken away from the first lever 9 7 2 by gravity, this configuration is not the first lever q 7 9, 铪 ^ 'The second lever 974 and the third lever 9 7 5 are shown in Figure 70. It is necessary to show that the whole Xinxin and Evil pivot together. The housing of the passenger aircraft is divided into ^, ^ ί 1-i-The relationship between the device and the trail connection mechanism is as shown in Figure 61 +, and the predetermined position of the device 950 is 100. The housing unit 100 is accommodated in the container. The mouth part 932 of the device 904, the large container 9 0 2, 90 3 and the mouth part 9 3 of the large container, as well as the mastocks in the eighth place of the small container. The guide mechanism of the flow path connecting mechanism 9 70 series, the angle of the mouth a y 1 3, 〇 q 9 At first, the β 100 is missing in the container! q Second, simply by setting the container housing unit, insert the nozzle 9 60 =,% and pivot the guide mechanism 97, the applicator can be inside. Into the fluid passage holes 9 1 3, 9 3 4 9 1 4, 9 3 5

313933.ptd

Page 101 544516 Brief description of the drawings [Simplified description of the drawings] Figure 1 is a front perspective view of a blood analysis device according to the present invention; Figure 2 is a back perspective view of a blood analysis device according to the present invention; Figure 3 Is a perspective view of a container housing unit attached to a blood analysis device according to the present invention; FIG. 4 is a view of a sample setting section of the blood analysis device according to the present invention, and FIG. 5 is a blood analysis according to the present invention Front view of the gripper claws of the device; FIG. 6 is a side view of the gripper claws of the blood analysis unit according to the present invention; FIG. 7 is in the direction of the arrow AA in FIG. 4; A longitudinal sectional view of the sample container in the sample rack of the invention; FIG. 9 is a schematic diagram illustrating the operation of the sample setting section of the blood analysis device according to the present invention; FIG. 10 is a schematic diagram illustrating the sample setting of the blood analysis device according to the present invention Section operation; Figure 11 is a schematic diagram illustrating the operation of setting a sample of a blood analysis device according to the present invention; Figure 12 is a front view of a detection section based on the blood analysis device of the present invention 13 before the first picture shows the root level of the pipette of the blood analyzer according to the present captive invention driving section view;

313933.pid Page 102 544516 Brief description of the drawings Figures 14 and 14 are views of the vertical sliding section of the pipette of the blood analysis device according to the present invention, and Figure 15 is a view in the direction of the arrow of BB in Figure 14; FIG. 16 is a front view of a vertical slide section of a pipette of a blood analysis device according to the present invention; FIG. 17 is a main view of a vertical slide section of a pipette and a horizontal drive section of the pipette according to the present invention Partial front view; Figure 18 is a left side view of the main part of the vertical sliding section of the pipette and the horizontal drive section of the pipe according to the present invention; Figure 19 is a vertical drive of the pipette according to the present invention The left side view of the section; FIG. 20 is a view in the direction of the arrow of CC in FIG. 19; FIG. 21 is a schematic view for explaining the operation of the vertical driving section of the pipette according to the present invention; and FIG. 22 is a schematic view. The operation of the vertical driving section of the pipette according to the present invention is explained; Fig. 23 is a front view of a part of the main part of the detector according to the present invention, and Fig. 24 is a detector according to the present invention. Partial cut-away side view of the main part, Figures 2 5 (a) and 2 5 (b) are a cross-sectional view and a plan view of a mixing chamber according to the present invention, respectively; Figure 26 is a plan view of a cleaner body according to the present invention; Figure DD arrow view;

313933.ptd Page 103 544516 Brief description of the drawings Figure 2 8 is a cross-sectional view of a negative pressure pump according to the present invention; Figure 29 is a system diagram of a fluid circuit of a blood analysis device according to the present invention, and Figure 30 The block diagram shows the circuit of the blood analysis device according to the present invention; Figures 3 1 (a) and 3 1 (b) are flowcharts for explaining the operation of the blood analysis device according to the present invention; Figure 32 is on page 26 Figure EE is a view in the direction of the arrow; Figure 3 3 is a schematic diagram showing the modification of the cleaner body corresponding to Figure 2 7; Figure 34 is a diagram for explaining the operation of the cleaner body shown in Figure 27; Figure 3 5 The figure is a schematic diagram for explaining the operation of the cleaner body shown in FIG. 27; FIG. 36 is a diagram for explaining the operation of the cleaner body shown in FIG. 27; FIG. The positional relationship between the suction pipe and the cleaner body shown in Fig. 6; Fig. 38 is a longitudinal section view of the pipette according to the present invention; Fig. 39 is a longitudinal section view showing another example according to the present invention Pipette 40; Figure 40 is a cross-sectional view of the pipette shown in Figure 39; Figures 41 (a) to 4 Figure 1 (e) is a schematic diagram illustrating the manufacturing process of the pipette shown in Figure 39; Figures 4 2 (a) to 4 2 (e) are schematic diagrams that are continuously displayed in accordance with the present invention

313933.ptd Page 104 544516 Screen image of a blood analysis device with a simple explanation; Figure 4 3 is a thumbnail showing an example main day surface (the main screen that is not visible when the whole blood mode is selected), and Figure 4 4 is a thumbnail Display another example of the main screen (the main daylight surface that is not visible when the pre-dilution mode is selected). Figures 4 and 5 are thumbnail illustrations of another example of the main screen (the main screen displayed when the measurement cannot be performed); An example of measuring the daytime surface is shown on the thumbnail (just after the analysis is started). Figure 4 7 is an example of the daylight surface display (shown after the WBC measurement is completed). Another example is the daylight surface measurement (Figure 4 8) (Shown after the measurement is completed) is another example of measuring the day and night; Figures 4 and 9 are thumbnails showing another example of the measurement screen used to display all analysis items (8 items); Figure 50 is a thumbnail display (for displaying (The statistical data measured by the WBC) is another example of the measurement screen; Figure 51 is a thumbnail display (for displaying the statistical data measured by RBC and PLT); another example is the measurement of the daytime surface; Figure 5 2 is a thumbnail display (when It will be displayed when the analysis item needs to be selected) Another example measurement screen; Figure 5 3 is a circuit diagram of the detection circuit of the blood analysis device according to the present invention; Figure 5 4 is based on the department used by the blood analysis device of the present invention Clo

313933.ptd Page 105 544516 Schematic description (C occkcr ft) of the power supply circuit; Figure 5 5 is an experimental circuit diagram to verify the performance of Cochlo power supply; Figure 5 6 is the use of market The circuit diagram of the boost circuit of the DC-DC converter is sold. Figure 5 7 is a line graph showing the relationship between the switching frequency and the output voltage; Figure 5 8 is a line graph showing the relationship between the capacitor capacitance and the output voltage; The figure is a line graph showing the relationship between the load current and the power supply voltage. Figure 60 is a waveform diagram obtained from the terminal device in Figure 54. Figure 61 is a schematic diagram showing a clamped container holder according to the present invention. Appearance of the container housing unit of the container housing; Figure 6 2 is a schematic view showing a large container structure used in the container housing unit according to the present invention; Figure 63 is a schematic view showing the structure of a small container used in the container housing unit according to the present invention Figure 64 is a sectional view of a large container fitted with an inner lid; Figure 65 is a sectional view of a small container fitted with an inner lid; Figure 6 is a perspective view showing two large containers and one small Container Figure 6 7 is a front view showing two large containers and a small container covered with a liner; Figure 68 is a plan view showing two large containers and a small container covered with a liner. status;

313933.ptd Page 106 544516 Brief description of the drawings Figures 6 and 9 are front views of the container holder to which the flow path communication mechanism is attached; Figure 70 is a schematic diagram illustrating the structure and operation of the guide mechanism of the flow path communication mechanism Figure 71 is a schematic diagram for explaining the structure and operation of the guide mechanism of the flow path communication mechanism; Figure 72 is a schematic diagram for explaining the structure and operation of the guide mechanism of the flow path communication mechanism; The structure and operation of the guide mechanism of the flow path communication mechanism are explained; FIG. 74 is a central sectional view of the guide mechanism; and FIG. 75 is a schematic diagram for explaining how a plurality of biasing elements are provided in the guide mechanism; FIG. 76 is a schematic diagram illustrating how a biasing element is disposed on the guide mechanism; and FIG. 7 is a schematic diagram illustrating how a biasing element is disposed on the guide mechanism. 1 Jtl liquid analysis device body 2 Case 3 Entry / display section 3 a LCD display 3b Transparent touch panel 4 Sample setting insert 4 a Convex 5 Coupling button 6 Sample setting section 7 Detection section 8 Fluid control Section 9 Electrical Control Board Section 10 Power Supply Section 11 Printer Section

313933_ptd Page 107 544516

Brief description of the drawings 1 2, 1 3 Ventilator (ventilation hole) 14, 15 Support shaft 16 Spring 17 Paw 18 Sample holder 19a, 19b Holder paw 21 Support plate 22 V-shaped notch 23 Bent part 24 Convex part 25 Locking hole 26a penetrated? L 26 Stopper 31 Suction flow path 32 Suction port 33 Stainless steel seal 50 Detector 51 First container chamber 52 Second container chamber 53 Third container chamber 54 Disk 55 Hole π 56 ^ 6 0, 61 Nozzle 57 Nozzle holder 58 Electrode 59 Liquid supply pipe joint 63 ~ 6 4 Joint 65 Liquid discharge pipe joint 66 Bubble injection pipe joint 67 1 White electrode 68 Light emitting diode 69 Light diode 70 Mixing chamber 71 Container portion 72 Diluent Supply fitting 72a Liquid supply part 73 Drain fitting 73a Liquid discharge port 74, 75 Pipe connection 74a Liquid discharge port 75a Air supply port 80 ^ 8 0a Cleaner body 81 Suction tube through 孑 L 81a Inlet 81b Outlet 82 Pipette guide holes 83, 8 3 a First through hole 84 Second through hole 31 3933.ptd Page 108 544516 Brief description of the drawing 8 5 a First opening 8 5 c Third opening 87 Cleaning liquid drain pipe joint 88 Cleaning liquid supply pipe joint 8 9 Second through hole 91 Rubber base 93 Suction pipe 95 Pipe joint 1 0 0 Container housing unit 2 0 1 Support plate 2 0 3 Active pulley 2 0 5 Pipette front and rear motor (step 2 0 6 rail 2 0 8 Horizontal moving plate 2 1 0 Coupler 3 0 0 Suction tube vertical sliding section 3 0 3 Suction tube holder 3 0 4 Longitudinal elongated guide grooves 306, 3 0 7 Notch 3 0 9 Liquid supply / Drain pipe connector 3 1 0, 3 1 1 Liquid supply / bleed 312, 3 13, 314 Pipe 317 convex part 3 1 9, 3 2 0 Screw 8 5b Second opening 8 6 a Ventilation path 8 7 a Cleaning liquid drain Putting path 8 8 a Cleaning liquid supply path 90 Air pump 92 Resin 箧 9 4 Vent pipe 96 Silencer vent pipe 2 0 0 Suction pipe horizontal drive section 2 0 2 Driven pulley 2 0 4 Timing belt motor) 2 0 7 3 0 2 Guide shaft 2 0 9 Slider 2 1 1, 2 1 2 Screw hole 3 0 1 Support 3 0 3 a End 3 0 5 Pipette holder 3 0 8 Guide roller tube joint 3 1 5 3 1 6 Screw 3 1 8 Spacer 4 0 0 Suction tube vertical drive section

313933.ptd Page 109 544516 Simple diagram Minger 401 main rod 4 0 3 screw cap 4 0 4b slider 4 0 5 suction tube up and down motor (step 4 0 6, 4 0 7 pulley 4 0 9 contact guide Lever 4 1 1 lock lever 5 0 0 control segment 5 0 0b drive control segment 5 0 0 d display color and change 5 0 0 d color change control segment 5 0 2 heme detector segment 510 area 5 1 2 All mode button 5 1 6 Start button 5 2 0 Menu button 5 2 4 Display the previous analysis sample test 5 2 6 Close button 5 3 0 Display the graph (triangle) 5 3 4 Back button ("top" button) 6 0 2 Suction port 6 0 4 U-shaped cutout 6 1 2 cube 614 trench 4 0 2 threaded shaft 4 0 4 a slide rail motor) 4 0 8 timing belt 4 1 0 cross recess 4 1 2 support plate 5 0 0 a Analysis section 5 0 0 c Color-changing section Color control section 5 0 1 Drive circuit section 5 0 3 Resistive detection section 5 1 1 Analysis mode section 5 1 4 Via dilution mode button 5 1 8 Paper P / F button 5 2 2 Quality control button 5 2 8 Delivery button 5 3 2 Lower right arrow button (forward button) 601 Suction flow path (fluid path) 6 0 3 Stainless steel seal 6 1 0 Stainless steel tube 6 1 3 stainless steel Stick 615 border

313933.ptd Page 110 544516 Simple illustration of the diagram 8 3 0 Cochlo power supply 831a Operational amplifier element 8 3 2 Switching circuit 832a, 832b, 832c, 832d 8 3 3 Booster 842 resistor 8 4 4 Enlarging Diode 8 5 5 Amplifier circuit 9 0 2, 9 0 3 Large container 9 0 6 Handle hole 9 1 1 Container mouth 913 Inner cover 9 1 4 Fluid passage hole 9 1 6, 9 3 8 Flow path tube 9 1 8 , 9 3 9 container side wall 9 2 0 convex part 922, 941 container convex part 9 2 4 small container mounting part 9 3 4 inner cover 9 4 0 concave part 9 5 0 container holder 9 5 2 bottom wall 9 5 4 left side Wall 9 5 6, 9 5 7 Set screws 8 3 1 Oscillator 8 3 1 b Inverting operational amplifier element Analog switching element 8 4 0, 8 4 1 Constant current circuit 8 4 3 Dummy resistor 8 5 2 Capacitor 901 inside 箧9 0 4 Small container 9 1 0 Groove (container body) 9 1 2, 9 3 3 Thread 9 1 3, 9 3 2 Mouth 9 1 5, 9 1 5 Ventilation hole 9 1 7, 9 3 6, 9 3 7 Shoulder 9 1 9 Neck 9 2 1 Flange 923 Shoulder protrusion 9 3 1 Container body 9 3 5 Fluid passage hole 9 4 2 Flat bottom 9 5 1 Front side wall 9 5 3 Rear wall 9 5 5 Convex portion 9 6 0 nozzle

313933.ptd Page 111 544516 Brief description of the diagram 961 Flow path 962 Nozzle tip 963 Support hole 964 Port 970 Guide mechanism 971 Supporting element (first shaft 972 first lever 973 second shaft 973 second supporting element (page 22 axes) 974 second lever 975 third lever 976, 9 77 torsion spring 978 convex part 979, 9 87 through hole 980 internal space 982 cloud-like recess 983 lever 984 pressing part 985 support convex part 988 concave 989 stop block 990, 9 丨 91 Central section 992 Arc cut-off section AD connector BIS B3 Containers C1 to C7 'Capacitor CL Feed lines D1 to D3 > Inner diameter D1 to D5 Diode Es Reference voltage Gdl Depth GW Maximum width J1 Insert Open / close sensor J2 Sample detection sensor J3 Sample adapter detection J4, J5, top position sensor of pipette J6 Pressure sensor J7 Float switch L Impedance coil PI Negative pressure pump P2 Liquid leakage Discharge pump P3 air pump PT, PT a pipette Qb Q2 Transistor R convex part adjacent to surface R1, R3, R4 resistor

313933.pid Page 112 544516 Simple illustration of Rs resistance SP1 sample container SfH, SR2, SR3 syringe pump SV1 to S V25 solenoid valve TP1 terminal S cleaner (pipette cleaning device) SP2 small sample container STM4, STM5 syringe pump Motors T1 to T2 output terminals WC waste chamber

313933.pul p. 113

Claims (1)

544516 6. Scope of patent application 1. An automatic sample analysis device, including: a pipette; a pipette driving device, which moves the pipette to a sample container at a predetermined position, and allows the pipette to be sucked by the sample container Sample, and then move the pipette to an open container set at another predetermined position to allow the pipette to discharge the sample to the open container; and an analysis section for analyzing the discharged sample; a pipette driving device includes a A main rod that can be moved in a vertical direction; a guide rod having less bending rigidity than the main rod; wherein when the sample is to be sucked by the sample container, the main rod moves the pipette vertically, and the guide rod guides the pipette to Open the f container, and then move the pipette vertically when the sample is to be discharged into the open container. 2. For example, the automatic sample analysis device of the scope of patent application, wherein the pipette driving device further comprises a pipette holder for holding the pipette; a horizontal drive section of the pipette, It is designed to support the pipette holder in a vertically sliding manner to drive the pipette holder horizontally; and a pipette vertical drive section is used to move the main rod and the guide rod vertically; The tube holder is buckled to the main rod in a horizontally detachable manner; wherein the pipette holder can be vertically moved by the main rod when it is snapped to the main rod, and when it is detached by the main rod, The guide rod moves vertically. 3. For the automatic sample analysis device according to item 2 of the patent application scope, the pipette holder has a convex portion, and the main rod has a concave portion, and the convex portion can be adjusted in the horizontal direction. 4. If the automatic sample analysis device of item 2 of the patent application scope, wherein the pipette holder includes a roller, the roller can be combined with a guide rod together with the guide rod. 313933.ptd Page 114 544516 6. Scope of patent application The rod moves together. 5. The automatic sample analysis device according to item 2 of the patent application scope, wherein the horizontal drive section of the pipette includes a vertical slide section of the pipette, which supports the pipette holder in a vertically sliding manner. 6. The automatic sample analysis device according to item 2 of the scope of patent application, which includes a quantitative pump connected to the pipette, which is used to suck the sample from the sample container after the pipette holder is lowered by the main rod. , And discharge the sample after the pipette holder is removed from the main rod. 7. For the automatic sample analysis device of the scope of patent application, the sample container may be a covered sample container. 8. For the automatic sample analysis device of the second scope of the patent application, wherein the vertical driving section of the pipette includes a stepping motor as a driving source, wherein when the pipette holder is moved in conjunction with the main rod, the former provides a step The driving current of the feeding motor for vertically moving the pipette holder is relatively large. 9. A pipette driving device, comprising: a pipette holder for holding a pipette; a pipette horizontal drive section which supports the pipette holding in a vertically sliding manner The holder is used for horizontally moving the pipette holder; a main rod, the pipette holder is buckled to the main rod in a horizontally detachable manner; a guide rod which is horizontally extended from the main rod; and a The vertical driving section of the pipette is used for vertically moving the main rod and the guide rod, wherein the pipette holder is vertically moved by the main rod when it is fastened to the main rod, and when it is removed by the main rod. The guide rod moves in a vertical direction. 1 0. —A kind of pipette stop device is used for pipette driving device, its package 3) 3933.ptd Page 115 544516 VI. The scope of patent application includes: a pipette vertical sliding section 5 which has a pipette holder for holding the pipette and a support element which can slide vertically The pipette holder is supported in a manner 9 a pipette horizontal horse section moving section, the pipette vertical sliding section is replaceably attached to the pipette horizontal driving section, and a stop element It is attached to the vertical slide section of the pipette to prevent the vertical slide of the pipette when the vertical slide section of the pipette is changed. 11. A sample container setting device comprising a frame having an inner diameter It is larger than the outer diameter 9 of the sample container used to hold the lower part of the sample container and the first and second elastic elements are used to elastically lose the sample capacity from the opposite sides. One side supports the sample container coaxially with the sample holder. 12. A sample holder locking device includes a sample holder that is movably supported to hold the sample container and a locking element that is used when the burette is inserted. The sample container held by the sample holder is mechanically prevented from moving in association with the pipette insertion operation. 13. A negative pressure pump 5 includes: • an air pump having an inlet V and an outlet U A pack cover having first and second -ω-shell perforations and covering an air pump. An extraction pipe is extended from the outside of the pack cover and communicates with the intake air V through a through-hole and a silent ventilation pipe. It is connected to the second through hole and projects to the outside. 14. A liquid mixing container includes a cylindrical outer surface, a bottom and a liquid. The body supply port V j-ru 5 is placed near its upper end to supply the liquid to its bottom along its inside. 9 The liquid mixing container 3l3933.ptd Page 116 544516 6. Scope of patent application Made of sex hawthorn f grease, and its inner surface is roughened. 1 5. A liquid suction tube comprising an elongated tube, the elongated tube having a liquid flow path extending parallel to its axis and extending inside the tube, and having all grooves arranged longitudinally extending on its outer surface. 16. A kind of pipette cleaning device, comprising: a cleaner body having a pipette penetrating path penetrating therethrough, a pipette being inserted from an inlet to an outlet; the pipette penetrating path containing suction The tube guide hole is formed at its inlet and coaxial with the tube, and a pipette cleaning hole is formed at its outlet 咅 [5 and coaxial with the tube; the pipette cleaning hole has first, second and third openings to thereby This sequence is formed on the inner surface from the inlet to the outlet; the cleaner body includes a ventilation path to communicate the first opening and the atmosphere, a cleaning liquid supply path to the third opening and a cleaning liquid discharge path to the first Two openings. 1 7. A liquid suction device, comprising: a suction tube having a suction opening arranged on a side wall thereof near a tip end thereof; a suction section for sucking liquid through the suction tube; and a cleaner A cleaning pipette is used for cleaning; the cleaner includes a penetrating path through which the pipette can be inserted, a cleaning liquid supply path is connected to the penetrating path for supplying cleaning liquid, and a cleaning liquid discharge path is connected to The through-path communication is used to discharge the cleaning liquid; wherein the cleaner is arranged so that the angle between the suction tube suction port port axis and the cleaning liquid discharge path inlet axis is greater than 90 when viewed in the direction of the suction tube axis. Degree angle. 1 8. An automatic sample analysis device capable of operating in multiple analysis modes. The automatic sample analysis device includes: an analysis mode selection button for selecting 313935.ptd Page 117 544516 VI. Choose one of the analysis modes for patent application; move the button together to output the instruction to start the analysis operation in the selected analysis mode; a color change section is used to change the color of the start button; a color change control The section is used to control the color changing section to change the color of the driving button according to the selected analysis mode; and an analysis section is used to analyze the sample when receiving an instruction from the startup button. 19. An automatic sample analysis device, comprising: an orifice through which the sample passes through the port; a DC power supply; a constant current circuit for supplying a constant current from the DC power supply to a sample passing through the orifice A resistance-type detection section for detecting the impedance of the sample passing through the orifice; and an analysis section for analyzing the sample based on the detected impedance change; wherein the DC power supply includes Cochlo (C 〇 ckcr ft) power supply. 20. A container, which is a first container used in combination with the second and third containers, comprising: a first body for containing a liquid, a first neck and a shoulder formed on an upper portion of the first body, the first The neck portion has a mouth communicating with the inside of the first body, and a first convex portion protruding from the first neck portion to the first shoulder portion, wherein the second container is formed in the same configuration as the first container and includes A second body, a shoulder portion of the second neck, and a second convex portion corresponding to the first container; the third container includes a third body for containing the liquid; the third body has two opposite concave portions formed on the outer side thereof, and A mouth portion and a third shoulder portion are formed on the upper portion of the third body, and a mouth portion communicates with the third notebook. 313933.ptd Page 118 544516 6. Inside the scope of the patent application, and the first container and the second container cooperate to hold the third container on the first and second shoulders, and by combining the first and second protrusions The third container is fixed to the opposite recess P and the third shoulder. 2 1. The container according to item 20 of the scope of patent application, wherein the first convex part includes an upper part [5 and a lower part, and the lower part is fitted into one of two opposite concave parts and an upper part covering the third shoulder part. 2 2. The container according to item 20 of the scope of patent application, which further comprises an inner lid fitted to the mouth of the first container and an inner suction tube, wherein the inner cover has a first through hole for communicating with the inner suction tube. And the second through hole is used to release air from the first body, and the pipe system is connected to the first through hole and extends to the inner bottom of the first body. 23. A container, which is a third container used in combination with the first and second containers, the third container includes: a third body for containing a liquid, the third body has two opposite recesses formed on its outer side, and A mouth portion and a third shoulder portion are formed on the upper portion of the third body. The mouth portion has one mouth and communicates with the inside of the third body. The first container includes a first body for containing liquid, and the first neck and shoulder portion are formed on the third body. In the upper part of the first body, the first neck has a mouth 咅 p communicating with the inside of the first body, and a first convex part is protruded from the first neck to the first shoulder part, wherein the second container is formed as Same configuration as the first container and includes a second body, a shoulder portion of the second neck, and a second convex portion. 313933.ptd Page 119 544516 6. The scope of patent application shall be in the first container, and the first container and the second container cooperate to hold the third container on the first and second shoulders, and by combining the first and second The two convex parts fix the third container to the opposite concave part [5 and the third shoulder part. 24. The container according to item 23 of the scope of patent application, which further comprises an inner lid fitted to the mouth of the third container, and an inner suction tube, wherein the inner cover has a first through hole for communicating with the inner suction tube. And the second through hole is used to release air from the third body, and the pipe system is connected to the first through hole and extends to the bottom of the third body. 2 5. A flow path connection mechanism comprising: a lever pivotally mounted on a support member; and a nozzle pivotally mounted on a lever, the nozzle has a proximal end and a distal end, wherein when the lever When pivoted, the proximal end is connected to the outer suction tube, and the distal end is connected to the mouth of the container. 2 6. The flow path connecting mechanism according to item 25 of the scope of patent application, wherein the lever includes first, second and third levers, the first and third levers are pivotally mounted on the supporting member, and the second lever And the nozzle are pivotally mounted on the first lever, wherein when the first, second and third levers are pivoted in the same direction, the first lever leads the distal end of the nozzle into the mouth of the container, and the second and third Use the lever f to close the container so that the nozzle remains connected to the mouth. 27. The flow path connection mechanism according to item 25 of the patent application scope, which further includes a biasing element for deflecting the lever to allow the lever to leave the mouth. 513933.pid Page 120 544516 6. Scope of patent application 28. For the container of scope 20 of patent application, the first body has a substantially rectangular shape. 2 9 · The container according to item 23 of the patent application scope, wherein the third body has a substantially flat bottom. 313933.Ftd Page 121