TW531389B - IVC rack system and method for detecting infectious particles within an IVC rack systems - Google Patents

Abstract

IVC rack system comprising a plurality of IVC cages 13 for test animals which are supplied with sterile air, wherein samples of exhaust from the IVC cages 13 are supplied from sampling points 15 to at least one sentinel cage 24 housing sentinel animals as bio-indicators for the detection of infectious particles within the exhaust air samples, and to at least one particle accumulating filter 33 for the identification of pathogens.

Description

531389 ___ Case No. 90127955_ Revised in January ____ V. Description of the invention (1) The present invention relates to an individual ventilation micro-isolation cage (IVC) grid system and a method for detecting infectious particles therein, more specifically It is said to improve the microbial monitoring of laboratory rats, gerbils, hamsters and house rats or other laboratory animals that are housed in a specific sterile environment. In the field of experimental medicine and laboratory animal science, individual ventilated microisolator-cages (IVC) grid systems are used to house experimental animals such as laboratory mice, gerbils, hamsters and house mice. In individual ventilated micro-isolation cage (IVC) grid systems, sterile animals are supplied with test air to improve the results of tests performed with test animals. Individual cages without special chamber mice and other important experimental objects, including the internal system, the chamber pre-filter high-efficiency particle Absorber isolation cage (Wenna π opeciiic pathogen-free, SPF) experiment House mice quickly accounted for basic and applied physiological and medical research. No specific pathogenic white rats, gerbils, hamsters, and domestic animals are allowed to move.2 The rearing and captive requirements are on the soil: the designated climate for the animals they raise. …, The representative of the city (IVC)-a group of individual micro-barriers, 1 animals in captivity in a closed environment without specific pathogens = significant 7: W know the individual ventilation micro-isolation cage (1 VC) grid preparation; the environment is empty 1Ventilation micro-isolation cage (IVC) grid system: (Pre-UUer 7-channel ^ device suction channel, a sub-absorber for absorbing particles (H i gh F = back through an intake air blower (HEPA)) Syria; flciency Particle IVC). These guys: filter, distributed to individual ventilation micro-tubes to 1U micro-isolated cage (I VC) through-

531389 _Case No. 90127955_ YYYY__ V. Description of the invention (2) The tube and the specific air supply diffuser installed on the individual ventilation micro-isolation cage (I VC) individually supply sterile air. These individual ventilation micro-isolation cages (I VC) contain experimental animals for physiological and medical investigations. The test animals breathe in sterilized air and produce exhaust gas, which is passively transported or pumped to a particle filter unit by a ventilator or to the building exhaust system. The particle filter unit shown in the first figure includes a pre-filter, a ventilator, and a high-performance particle absorber (HEPA) filter that filters exhaust gas generated by test animals in individual ventilation micro-isolation cages (I VC). The filtered exhaust gas must then be reintroduced into the grid system or sent out to the indoor environment. The second figure shows a more detailed diagram of the individual ventilation micro-isolation cage (I VC) grid system according to the known techniques. As shown in the second figure, the individual ventilation micro-isolation cages (I VC) according to the known art. The grid system includes a plurality of individual ventilation micro-isolation cages (I V C) in different columns. Sterile feed air is transferred via a vertical air supply chamber to different horizontal supply manifolds connected to individual ventilation micro-isolation cages (I V C). Exhaust gas from individual ventilation micro-isolation cages (I VC) in the same row is output through a vertical exhaust manifold. In a ventilated micro-isolated cage (I VC) grid system, all individual vented micro-isolated cages (I VC) are individually supplied with sterile air, and there is no air between the individual vented micro-isolated cages (I VC). exchange. For physiological and medical investigations, test animals in individual ventilated micro-isolation cages (IVC) must be removed by the investigating scientist. During the investigation of experimental animals, the investigated experimental animals may be infected by viruses, bacteria or parasites. Infected animals are returned to individual ventilation micro-isolation cages (I VC) and transmitted to other experimental animals. Another possible route of infection in test animals is a defective inlet particle filter unit that does not produce sterile feed air. With other laboratories

531389 _Case No. 90127955_year month__ V. Description of the invention (3) The exchange of laboratory test animals is another possible source of infection for experimental animals. It is also possible that the test animals were already infected when they were first placed in individual ventilation micro-isolation cages (I V C). These potentially infected test animals develop an open or acute infectiousness and illness and are transmitted to other test animals in the same ventilated micro-isolation cage (IVC). Since there is no air exchange between the individual ventilation micro-isolation cages (I VC), it is not useful to monitor the experimental experimental animal population by investigating the sampling animals taken from different individual ventilation micro-isolation cages (IVC), because there is still no Infectious agents were detected in other individual ventilation micro-isolation cages (I VC). In the case of test animals in individual ventilation micro-isolation cage (IVC) trellis systems suffering from new infectious agents, the infectious agents of the test animal population are likely to be moved and replaced with grass mats before being detected Intermediate spread. Therefore, one object of the present invention is to provide a method for detecting infectious particles in any cage of an individual ventilation micro-isolation cage (I VC) grid system and an independent ventilation micro-isolation cage (I VC) grid system. The infectious agents in the individual ventilation micro-isolation cage (IVC) grid system were detected in a very short period of time. This objective is achieved by an individual ventilated micro-isolation cage (I VC) grid system with the characteristics of item 1 of the patent application. The invention provides an individual ventilation micro-isolation cage (I VC) grid system, which includes: a plurality of individual ventilation micro-isolation cages (I VC) for test animals that are supplied by sterile air; and the individual ventilation micro-isolation A sample of exhaust gas from the IVC is supplied from a sampling point to at least one sentinel cage, which houses observation animals used as biological indicators to detect infectious particles in the exhaust gas samples ( sentine 1 anima 1).

531389 _Case No. 90127955_ YYYY__ V. Description of the Invention (4) In a preferred embodiment, the observation animals are used as biological indicators for detecting infectious pathogens. Such infectious agents are preferably airborne. In a preferred embodiment, the individual ventilation micro-isolation cages (I VC) and observation cages include a pressure gauge for controlling air pressure in the individual ventilation micro-isolation cages (I VC) grid system. In another preferred embodiment of the individual ventilation micro-isolation cage (IVC) grid system according to the present invention, the observation cages include an inlet airflow adjustment cock for adjusting the outflow of exhaust gas samples into the observation cage. . In a preferred embodiment, the observation cage includes an outlet airflow adjusting cock for adjusting the amount of exhaust gas discharged from the observation cage. The sterile air is preferably supplied through a manifold and sent to the individual ventilation micro-isolation cage (I VC) by an air supply diffuser mounted on the individual ventilation micro-isolation cages (I VC). In a preferred embodiment, there is an inlet particle filter unit for generating sterile air. The unit includes a pre-filter screen which is supplied with indoor ambient air and a pre-filter screen connected to the pre-screen filter. High-performance particle absorber (HEPA) filter that produces sterile air from indoor ambient air. In another preferred embodiment of the individual ventilation micro-isolation cage (IVC) grid system according to the present invention, there is an outlet particle filtering unit, which includes a filter for the individual ventilation micro-isolation cages (I VC). The pre-filter of the exhaust gas and the exhaust of the observation cage, and a high-performance particle absorber (HEPA) filter connected to the pre-filter. The test and observation animals are preferably laboratory rats, gerbils, hamsters or house rats.

531389 _Case No. 90127955_ Modification of the month _V. Description of the invention (5) In a preferred embodiment of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention, the individual ventilation micro-isolation The cage (I VC) grid system is a single-sided individual ventilation micro-isolation cage (I VC) grid system. In an alternative preferred embodiment of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention, the individual ventilation micro-isolation cage (I VC) grid system is a double-sided individual ventilation micro-isolation Cage (IVC) grid system. In a preferred embodiment of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention, each row of individual ventilation micro-isolation in the individual ventilation micro-isolation cage (I VC) grid system The cage (I VC) provides a sampling point. In another preferred embodiment of the individual ventilation micro-isolation cage (IVC) grid system according to the present invention, a sampling point is provided in a vertical exhaust chamber of the individual ventilation micro-isolation cage (IVC) grid system . In an alternative embodiment, a sampling point is provided in each individual ventilation micro-isolation cage (I VC) of the individual ventilation micro-isolation cage (I V C) grid system. The advantage is that it can detect the exact location of the source of infection in the individual ventilation micro-isolation cage (I VC) frame system. The following describes a preferred embodiment of an individual ventilation micro-isolation cage (IVC) grid system and a method for detecting infectious particles therein with reference to drawings to explain the basic characteristics of the present invention. The first picture is a block diagram of an individual ventilation micro-isolation cage (IVC) grid system based on conventional techniques. The second picture is a diagram of an individual ventilation micro-isolation cage (IVC) grid system based on conventional techniques. Structure diagram

531389 _Case No. 90127955_ 年月 日 __ V. Description of the invention (6) The third diagram is a block diagram of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention, and the fourth diagram is a A preferred embodiment of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention; FIG. 5 is another preferred implementation of the individual ventilation micro-isolation cage (I VC) grid system according to the present invention Figure 6 shows an intake well of an individual ventilation micro-isolation cage (IVC) grid system according to a preferred embodiment of the present invention; and Figure 6 B shows an individual ventilation according to a preferred embodiment of the present invention Exhaust well for air micro-isolation cage (I VC) grid system. A preferred embodiment of the present invention is shown in block diagram in the third figure, wherein the individual ventilation micro-isolation cage (I VC) grid system 1 includes an air inlet 2 to supply indoor ambient air to a front through an air pipe 3 The filter 4 absorbs particles in the indoor ambient air. The indoor ambient air is sucked into the front screen 4 by a ventilation device 5 connected to the output side of the front screen 4 through a pipe 6. The ventilation device 5 transfers the air filtered by the pre-filter through a pipe 7 and an airflow adjusting cock 8 to a high-performance particle absorber (HEPA) filter 9. The airflow adjusting cock 8 is controlled by a control unit through a control line 10. The pre-filter 4, the ventilation device 5, and the high-performance particle absorber (HEPA) filter 9 are components of an inlet particle filter unit 11. The inlet particle filtering unit 11 generates sterile air from the ambient air in the room. The sterile air passes through the manifolds 12a, 12b ... 12η and is supplied by the air supply diffuser to the individual ventilation micro-isolation cage (IVC) 1 3a, 1 3b ... 1 3 η 〇 individual ventilation micro-isolation cage (I VC) 1 Line 3 is used to breed and house experimental animals, such as laboratory rats, gerbils, hamsters or house rats. Individual ventilation micro

Page 10 531389 _Case No. 90127955_ Year Month__ V. Description of the Invention (7) The exhaust gas generated in the isolation cage (I V C) 1 3 is transferred to an sampling point 15 through the exhaust manifold. The exhaust gas sample is taken from sampling point 15 through a sampling air pipe 16 to an inlet 17 of an infectious agent monitoring unit 18. The infectious agent monitoring unit 18 includes an input pipe 19 connected to a ventilation device 20 to extract an exhaust gas sample from the sampling point 15. The ventilation device 20 is controlled by a control unit through a control line. The ventilation device 20 is connected to a tube 21 having an anemometer 22 therein, and the tube 21 is connected to an observation cage 24 with its output side. The anemometer 22 is connected to the control unit through a measuring line 25. The air sample taken in by the ventilation device 20 is sent to an observation cage 24 through a tube 21. The observation cage 24 contains observation animals for detecting biological indicators of infectious particles in the exhaust gas sample sent to the infectious substance monitoring unit 18 through the sampling air pipe 16. Infectious airborne pathogens from test animals in individual ventilation micro-isolation cages (IVC) 1 3 are sent to observation animals in observation cages 24. These observation animals are preferably of the same species as the monitored test animals in individual ventilated micro-isolation cages (IVC). These observation animals must be specially bred for use as biological indicators for detecting infectious particles in exhaust gas samples. It is best to choose or breed it so that it is very sensitive to infectious agents. The infectious agent monitoring unit 18 further includes an exhaust pipe 2 6 with an anemometer 27 inside. The anemometer 27 is connected to the control unit via a measuring line 29. The exhaust pipe 2 6 connects the observation cage 24 with a ventilation device 30, which is controlled by a control unit through a control line 28. The exhaust gas leaving the ventilation device 30 through the output pipe 26 passes through a pipe 31 to the outlet 32 of one of the infectious agent monitoring units 18. The infectious agent monitoring unit 18 preferably further includes a particle accumulation filter 3 3 connected to the input pipe 19 through a pipe 34. A ventilation device 52 is controlled by a control unit through a control line 53 and is installed in the pipe 34, and an anemometer 54 is connected to the control unit through a side measuring line 5 5 and is also installed in the pipe 34. Particles accumulate

Page 11 531389 _Case No. 90127955_Year Month__ V. Description of the invention (8) The exhaust gas of the filter 3 3 is introduced into the tube 31 through a tube 51 and then leads to the output tube 36 through the tube 35. The particle accumulation filter 33 can be used to collect infectious particles leading to the infectious substance monitoring unit 18. By incubating the particle accumulation filter 3 3 on a bacterial culture medium, the collected bacteria can be cultivated, investigated, identified, and characterized. The exhaust gas from the infectious agent monitoring unit 18 is sent from an exhaust gas outlet 3 2 through a pipe 35 to an exhaust pipe 36. In a preferred embodiment, as shown in the third figure, the exhaust pipe 36 is connected to an outlet particle filter unit 37 including a pre-filter 38. The front screen 38 has a pipe 39 on its exit side, through which exhaust gas is sucked in by the ventilation device 40. The outlet particle filtering unit 37 further includes an air-conditioning cock 41 controlled by a control unit through a control line 42. The exhaust gas drawn in by the ventilation device 40 is sent through a pipe 44 to a high-performance particle absorber (HEPA) filter 43. A high-performance particle absorber (HEPA) filter 43 absorbs particles and discharges the filtered exhaust gas through a tube 45 to one of the exhaust ventilation outlets 46 of the individual ventilation micro-isolation cage (I VC) grid system 1. Individual ventilated micro-isolation cages (I VC) 1 and observation cages 2 4 contain pressure gauges 47, 48 for measuring the pressure inside the cage. The pressure gauges 47 and 48 generate pressure measurement signals and send them to the control unit via the measuring lines 49 and 50. With pressure gauge 47, ventilation devices 5 and 40 and air conditioning cocks 8 and 41 all connected to a control unit, individual ventilation micro-isolation cages (IVC) 1 3 a, 1 3 b ... 1 3 η pressure levels To be controlled. With the pressure gauge 48, the ventilation devices 20 and 30 and the anemometers 22 and 27 all connected to a control unit, the pressure level in the observation cage 24 can be controlled. By means of a ventilation device 5 2 and an anemometer 5 4 connected to a control unit, the particles are filtered through the accumulation

Page 12 531389 _Case No. 90127955_ Year Month Amendment_ V. Description of the Invention (9) The air volume of the device 3 3 can be controlled and quantified. In normal operation, the pressure in the test individual ventilation micro-isolation cage (I VC) 1 3 and the observation cage 2 4 is controlled to be greater than the ambient air pressure. This ensures that no ambient air enters the individual ventilation micro-isolation cage (I VC) 1 3 and the observation cage 2 4. However, when testing with highly infectious substances, the pressures in the test cages 13 and 24 are controlled by the control unit to be lower than the ambient air pressure. This ensures that the air in cages 1 3, 2 and 4 will not leave the individual vented micro-isolation cage (I VC) grid system endangering scientists in the room or the environment. Periodically or when the behavior of the observation animals is different, the observation animals in the observation cage 24 are taken out from the observation cage 24 and surveyed. The check is made to see if the animal produces antibodies or changes in its body tissue. These results are additionally compared with infectious particles sampled by the particle accumulation filter 33 of the infectious substance monitoring unit 18. The fourth figure shows a schematic front view of a single-sided individual ventilation micro-isolation cage (I VC) grid system 1 according to the present invention. In this embodiment, an infectious agent monitoring unit 18 a for monitoring infectious agents in the cage 13 a is provided in the first column of one of the grid systems 1. For each row of the grid system 1, a row of infectious substance monitoring units 18a is connected to it through a row of sampling points 15a and a sampling gas supply pipe 16a. In addition, the grid infectious substance monitoring unit 18 b is connected to a grid sampling point 15 b to receive exhaust gas samples from all cages in the individual ventilation micro-isolation cage (I V C) grid system 1. Each column of the grid system 1 includes a plurality of individual ventilation micro-isolation cages (I VC) 13, which are individually sterile from the horizontal air supply manifolds 12 a, 12 b, 12 c. Air supply. All the cages 13 in the same row output the exhaust gas generated by the horizontal exhaust manifolds 14a, 14b, and 14c connected to one of the vertical exhaust chambers of the individual ventilation micro-isolation cage (I VC) grid system 1.

Page 13 531389 _Case No. 90127955_ Revised Year of the Month _ V. Description of the invention (10) Each column is equipped with an infectious substance monitoring unit 1 8 a makes it possible to locate individual ventilation micro-isolation cage (IVC) grid systems And take necessary steps to control or eliminate infectious agents. The fifth figure shows a schematic front view of a unilateral individual ventilation micro-isolation cage (I VC) grid system with factory-set infectious substance monitoring units 1 8 c and 18 d. As shown in Figures 4 and 5, the present invention may be applied as an additional device of the conventional individual ventilation micro-isolation (I VC) grid system or as an individual ventilation micro-manufactured to include an infectious agent monitoring unit 18 Isolation cage (I VC) grid system. FIG. 6A and FIG. 6B are side views of a double-sided individual ventilation micro-isolation cage (I V C) grid system according to the present invention. As shown in FIG. 6B, the individual ventilation micro-isolation cage (I VC) grid system 1 is provided with a row a for sampling points 15 a. Each row of the individual ventilation micro-isolation cage (I V C) grid system 1 must have a row of sampling points. In addition, there is a grid sampling point 15b for monitoring the exhaust gas of the entire grid system 1. The additional side sampling points 47a, 47b make it possible to determine which side of the individual ventilation micro-isolation cage (IVC) grid system 1 is infected. In an alternative embodiment, each of the individual ventilation micro-isolation cages (I VC) of the individual ventilation micro-isolation cage (I VC) frame system 1 is connected to an infectious agent monitoring unit 18. According to this embodiment, it is possible to immediately detect the exact infection location in the I VC grid system 1. The individual ventilation micro-isolation cage (IVC) grid system 1 according to the present invention facilitates rapid detection of captives in the individual ventilation micro-isolation cage (I VC) 1 3 by using observation animals in the observation cage 24 New infectious agents of airborne pathogens in experimental animals. These observation animals were only exposed to exhaust gas from individual ventilated micro-isolated cage (IVC) test animal cages 13.

Page 14 531389 _Case No. 90127955_ Revised Year of the Month _ V. Description of the Invention (11) To detect individual ventilation micro-isolation cage (IVC) grid system 1 for individual ventilation micro-isolation cage (I VC) 1 3 Infectious particles inside, the ambient air was first filtered to produce sterile air and then supplied to individual ventilated micro-isolation cages (I VC) 1 3 in which the test animals were housed. Infected test animals spread infectious airborne pathogens from their exhaust gases. At least one observation cage with observation animals 2 4 is supplied with the individual ventilation micro-isolation cage (I VC) 1 3 exhaust gas. The infectious particles in the exhaust gas are detected by observation animals as biological indicators. The sampling tube leading to the exhaust well conveys part of all the exhaust gas generated by all the test animals in the individual ventilation micro-isolation cage (I VC) 1 3 to the infectious agent monitoring unit 18. Air samples containing latent airborne pathogens from experimental animals were drawn into the observation cage 24 by the ventilation device 20. The animal's exhaust gas was sucked by the ventilation device 30 and fed back to the exhaust well of the individual ventilation micro-isolation cage (I VC) grid. Samples are taken from a predetermined group of individual ventilated micro-isolated cage (I V C) grid system 1 test animal cages, and exhaust gas from experimental test animal cages 13 must be sampled by row sampling, side sampling, or grid sampling. Row sampling is performed before the end of each horizontal exhaust manifold to the point where the exhaust gas enters the vertical chamber of the grid system. Side sampling is performed at the end of each vertical air cell in a double-sided grid. Grid sampling is performed from the exhaust chamber combined before the exhaust gas is drawn into the outlet particle filter unit 37 or the building exhaust system. The air pressure and air flow rate in the individual ventilation micro-isolation cage (I VC) grid system 1 are controlled by a control unit that controls an air regulating valve. One low-speed continuous airflow in the individual ventilation micro-isolation cage (I VC) grid system guarantees each independent individual ventilation micro-isolation cage (I VC) 1 3 continuous ventilation per hour 0

Page 15 531389 _Case No. 90127955_ Year, Month, and Day Revised Schematic Brief Description 1: Individual ventilation micro-isolation cage (I VC) grid system 2: Air inlet 3: Air pipe 4, 38: Pre-filter 5, 20, 30, 40, 52: Ventilation device 6, 7, 21, 31, 34, 35, 39, 44, 45, 51: Pipe 8: Airflow regulating cock 9, 4 3: High-performance particle absorber (HE PA) Filter 1 0: Control line 1 1 ·· Inlet particle filter unit 12a, 12b ... 12n, 14a, 14b, 14c: Manifold 1 3a, 13b ... 13η: I VC cage 15, 15a, 15b, 47a, 47b: Sampling Points 16 and 16a: Sampling air pipe 17: Inlet 19: Input pipes 18, 18a, 18b, 18c, 18d: Infectious agent monitoring unit 22, 27, 54: Anemometer 2 4: Observation cage 2 5, 2 9, 4 9, 5 0, 5 5: Measurement line 2 6: Exhaust pipe 2 8, 4 2, 5 3: Control line 32: Outlet 3 3: Particle accumulation filter 36: Output pipe

Page 16 531389 Case No. 90127955 Amendment Brief description of the drawing 3 7: Outlet particle filter unit 41: Air conditioning cock 46: Exhaust gas outlet 4 7, 4 8: Pressure gauge Page 17

Claims (1)

531389 Case No. 90127955 Amended on 1st and 2nd month, including its C, C, D, C, KONE, 3, C, and 4 racks. The scope of this patent application is an individual ventilation micro-isolation cage (IVC) grid system (1 ), Which includes the mouth pressure of the cage C) The gas measured by the cage 3) The inside of the IV internal observation (1C to view the original; for}} As little as the cage Yuan 2424 C) From less gas to single} c C IV separates the 15 cages, C micro-waits, etc. C measures the cage gas for the purpose, detects a point of view and exchanges, and then measures to separate the sample from the 15-segment separation. C crops pass from the air to the air, etc. It should be changed as soon as possible.} The capital system will control the amount of 48 views in the system to control one. The C test of the circle is used in the formula. The target device will be used to measure the object's sensitivity sheet C, which is measured by a cube in the sample cage and the dynamic supply of the element 20. The test vitality force is set to 30, and the pressure of the test is to control the installation of C bacteria. The number of wind set in one is}} the number of grains is less than the number of winds. The tone is 1X OAM 1i tnj. Uth. Circulation color, etc.} I Xuan 7 Li f 4 In production C Special L Please refer to J 丨, please apply for the senses, please refer to the application on the shelf with} such as ^ Yuan single system control For Π cage resources; 1 empty measure of the perimeter of the perimeter & observation & no β. J should be j should be provided by the junior middle school, please, please, single, Shen Zhongzheng led Ms. U Tong Change the air cage of the control system, and change the micro-air item every V micro C The observation cage (24) in the individual ventilation micro-isolation cage surrounding any one of the grids includes an inlet wind speed supply measurement data. The individual ventilation micro-isolation cage (I VC) grid (24) of this item includes an outlet anemometer (27) material. The individual ventilation micro-isolation cage (I VC) grid of the project is supplied through the manifold (1 2) and installed by the installation. Page 18 531389 Case No. 90127955 Revised Λ-JnuD Tone II for micro-gas exchange in exchange for profit-Τ Specially please solidify ψ Six in the feeder to diffuse the air supply cage β Micro-gas exchange for another system Department • Mouth K 6 Fancai Fanli specially requested Shenruge 1304- C Mouth- ¥ v't C Micro-air-inlet ventilation through the cage and separators, air-free production of a bacteria, a spare item Before the filter is placed in the filter, the filter should be used for self-ventilation before it is used for air use} Ring 4 The internal chamber should be placed before the filter. It should be taken from c. Empty bacteria without \) / Health 9 C-producing air filter air filter transit ring} In the PA room HE, the special application of C is as follows ^ ^ > 'wuqi ° toL α ^ waste Vhai / I t of (, Cage > 3) Li 371 3 / (V Separator C Microelement C) Gas list IV Change 遽 C Do n’t go through the cage to separate the septum mouth slightly out of the air to prepare for a change ^ L] It is a high-efficiency exhaust gas filter with a mesh filter to collect the pre-sucking particles. 4 2 (8 3 C The Gukou Yu connector once filtered and 8 a9. Fan Li specially requested to apply the system such as the mouse hamster C mouse V white IC chamber. The inspection and separation of the cage is a micro-pneumatic change test. For the test, please apply for the items such as the pedestal frame or the house of the rat's house. For each cage, change the gas to another item. Change the system frame for Tigging points. For example, if you want to change the height of the C frame of the 1st system, you can change the point "like" L for J, then for one, for the 1 side. ra Lee in Μ Junior High School < * !? Please ask him \ Shen, the cage 0 separated by micro gas lattice Λ-1 J month β off the frame Page 19 531389 _Case No. 90127955_Year_Month__ VI. Patent Application Range 1 1. For example, the individual ventilation micro-isolation cage (I VC) grid system of the scope of patent application, where the individual ventilation Micro-isolated cage (IVC) grid system (1) Each individual ventilated micro-isolated cage (I VC) (1 3) provides a sampling point (1 5) 〇 12. For individual replacement of the scope of patent application item 1 An air micro-isolation cage (I VC) grid system provides a particle accumulation filter (3 3) to sample infectious particles supplied to the observation cage (2 4). Page 20