TW449662B - Microscope image transmitting system, method of processing microscope images, and recording medium for use in processing microscope images - Google Patents

Abstract

This invention is a microscope image transmitting system which, in long-range observation of microscope images, can carry out designation of mesh dissection of observation ranges at an initial image observation stage in an efficient and accurate manner. That is, this invention is a microscope image transmitting system that designates a range of a specimen image to be observed at a high magnifying power with block units, and that includes a video camera (6) for photographing a specimen to obtain the specimen image, and a personal computer (1, 5) for recording stationary images, including the photographed specimen image, detecting the position of the specimen image from the stationary images, dissecting only the range with the specimen image into designated block units, and controlling lines indicating the blocks to be listed in a monitor (2, 4) and to overlap with the stationary image for display.

Description

449662

V. Description of the invention (1) The invention is printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economics, which is related to-for example, the microscope images related to the processing of specimens, 'for observation at a distance, especially, there is about Among the above specimens, the area to be expanded is designated by a predetermined section (unit), and the segment can be further expanded_micro image transmission system, microscope image processing method, and recording medium used for microscope image processing . 'Xinzhi' uses a video camera to take a sample placed on the stage of a microscope, and then takes it through the image capture port of a personal computer 'and digitizes the image, for example, through a public line such as ISDN Various technologies have been developed for transferring to a personal computer configured for long-distance processing, and a microscope image transmission system in which the monitor of the personal computer displays images. The microscope image transmission system is used, for example, in pathological diagnosis of a pathologist, and as the microscope operation or magnification is changed by a remote pathologist, the stage movement becomes feasible. For example, in Japanese Patent Application Laid-Open No. 9-120031, it is disclosed that a transmitting terminal personal computer (dependent side) and a receiving terminal personal computer (observation side) are connected via an isdn line to calculate the microscope position of the designated photography position A technology that takes the absolute coordinates of the object and takes the calculated position as the basis to control the position of the object, and takes the image to be observed as its feature. Generally, pathological observation using a microscope is based on first observing the overall image of a microscope specimen by a pathologist and setting a summary observation policy, then performing microscopic observation at a low magnification, and performing an initial diagnosis or a higher magnification. Detailed observation, however, in the above system, such a series of diagnostic steps can also be achieved at a long distance (observation side). This paper is suitable for Chinese Standards (CNS) A4 specification (Q χ 297 meals) 4 ----— — — — — — — — — .ίΛ {Please read the precautions for your noodles before filling out this page) Intellectual Property Bureau of the Ministry of Economic Affairs, W Industrial Consumption Cooperation, ittn A7 ______B7____ V. Description of Invention (2) Others As for the aspect, in Japanese Unexamined Patent Publication No. 6_22228 1, it is revealed that when a microscope observation is performed at a low magnification, a specimen image is divided into meshes in a predetermined rectangular area, and a desired area is selected from the divided areas, that is, to A system that automatically captures images for this selected area as its features. Also, in Japanese Patent Application Laid-Open No. 9-1383 55, it is revealed that the specimen image is binarized (binary) to the threshold value set and set, and the unnecessary area is removed in the field of mesh division. Paragraph is the technology that characterizes it. However, using the technique disclosed in the aforementioned Japanese Unexamined Patent Publication No. 6-222281, the specimen image is divided into meshes, and whether or not the divided portions need to be taken into the image can be confirmed. However, on the other hand, the operation of designating the start and end points of the field to be divided by the operator and the operation of removing unnecessary parts after the division of the mesh are accompanied by a problem of complicated operation. In particular, in terms of surgical diagnosis, it is best not to waste time '. Therefore, there is a demand for improvement. In addition, in the technique disclosed in the above-mentioned Japanese Patent Application Laid-Open No. 9-138355, only the start point and end point of the mesh taking position are designated, and the unnecessary places can be automatically deleted according to the setting of the limit value. However, the section designation must follow the fixed coordinates (x, y). Therefore, there is a problem that the most suitable section designation cannot be made. Also, it is necessary to identify unnecessary data such as gray dust, and it is impossible to specify the correct mesh division designation. Possibility exists. That is, in the case of Acer images, because of the large range, the end images of microscope specimens may be taken, and even the stage clamps for microscope specimens and microscopes may be taken. Space between specimens = '297 mm from the macro image intended to be divided into meshes with a low magnification ------------- install i — (please read －f 面 tii first Matters needing attention * 4Fill this page) Order:-Line A7 B7 V. Invention description (3), there is a high possibility that unwanted image parts will occur, and these unwanted parts are identified as specimen images' because The result of the mesh segmentation will cause errors', which is required to solve the problem. ^ The present invention has been made in view of the above-mentioned problems, and its purpose is to provide the designation of the mesh division of the observation field on the initial observation image in the long-distance observation of the microscope image, with simple operation, efficient and accurate. Microscope image transmission system, microscope image processing method, and recording medium used for microscope image processing. In order to achieve the above object, in the first aspect of the present invention, a photographing device that obtains the specimen image in a predetermined section unit in a specimen image in an area to be observed at a high magnification will include the photographing device described above. A recording device for recording a still image of a photographed specimen image, a display device for displaying the still image recorded on the recording device, and a position where the specimen image is extracted and used as a basis, and only the specimen image exists. The field is divided by a predetermined section unit, and an indicator indicating the section is superimposed on the still image, and has a control device for displaying the function on the display device. In the second aspect, the microscope image transmission system will be used in the specimen image to be observed at a high magnification and designated by a single position in the specified section. It is a photographing device equipped with a photographic specimen to obtain the aforementioned specimen image. A recording device that records a still image containing a specimen image taken by the above-mentioned photographing device, at least also a display device that wants to display the still image recorded on the recording device, and takes the still image without the above-mentioned specimen, which is smaller than the above Take a still image in the state of the specimen, and use the difference between the luminance information of the two. The paper & degree is applicable to the S1 National Standard (CNS) A4 specification (21CU 297 mm). Please fill in this page for the matters) Order-Printed by the Consumers' Cooperative of the Wisdom Time and Industry Bureau of the Ministry of Economic Affairs 6 Printed by the Intellectual Property Bureau of the Ministry of Economy ΜΓ Industrial and Consumer Cooperative Cooperatives A7 ------B7____ 5. The invention description (4) is the foundation The detection device that detects the insufficient light amount and excludes the insufficient light amount detected by the detection device, and then detects the specimen image from the still image. Only the area in which the specimen image exists is divided by the specified single position of the section, and the private information related to the section is superimposed on the still image, and the display control on the display device is controlled Device. In the third aspect, the observation side and the dependent side, which have control devices at least on either side, are connected so as to be able to communicate freely with each other, and will be observed in the specimen image at a high magnification, in order to The microscope image transmission system is divided by a predetermined segment unit, and the dependent side is provided with a photographing device for photographing a specimen to obtain the specimen image, and displaying a still image including a still image of the specimen image photographed by the photographing device. Device, and a first transmitting and receiving device capable of transmitting and receiving information to and from the observation side, and the observation side is a second display device for displaying a still image transmitted from the relying side, and is dependent on the above A second transmitting and receiving device capable of transmitting and receiving information is provided on the side, and the above-mentioned control device has a position for extracting a specimen image by displaying a still image of the display device using the control device, and using it as the basis, only The area in which the specimen image exists is divided by a predetermined section unit, and an indicator indicating the section is superimposed on a still image for display, and The still image using the display device of one of the above control devices can also be the same image as the still image displayed by the display device of one of the above control devices. At least one party can send the information about the location information of the specimens of the above-mentioned area & at the same time, and in the g1 ^ T297 prostitute) ---. ------ II (Please read- Note on the f side, please fill out this page again} Ordering line · 449662 A7 B7 V. Description of the invention (The above-mentioned relying side and observation side share the same information, which is its feature. Please read the notes on the back before filling out this buy in the fourth state Sample 'microscope image processing method has the steps of extracting the luminance information of the still image containing the specimen image, and setting the maximum luminance level based on the luminance information related to the background position of the specimen image. The step of setting the minimum luminance level for the luminance information related to the data position of 'is based on the above maximum luminance level and minimum luminance level, The step of transforming the above-mentioned luminance information, and determining the background position of the specimen image 'unwanted data position, the position of the specimen image based on the transformed luminance information as a basis, and extracting the position of the specimen image from this In the fifth aspect, the microscope image processing method has a step of extracting color information of the specimen image, identifying the position where the color information is close to the maximum value and the background position of the specimen image, and bringing the color information close to The step of identifying the position of the minimum value from the unnecessary data position of the specimen image, and the step of identifying the position of the specimen image by the above-mentioned color information to a position above a predetermined threshold, and extracting the position of the specimen image The Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs printed in the sixth aspect. The microscope image processing method has the steps of extracting different color information at the same position of the specimen image, and finding the color at the same position of the specimen image The step of the position where the information difference becomes the largest, and the threshold value is set based on the value of the difference between the color information as the maximum. Steps, and distinguishing the above color information with the above threshold value, will be a step above the threshold value or below the threshold value to identify the position of the specimen image and extract the position of the specimen image. In the seventh aspect, the recording medium used for microscope image processing is the paper size applicable to the Chinese National Standard (CNS) A4 specification (210 * 297 mm). The cooperation of the employees of the Nian Zou Intellectual Property Bureau. Tuyinzhi A7 B7 V. Description of the invention (6) After giving the brightness information of the still image containing the specimen image, the maximum brightness level is set based on the brightness information related to the background position of the specimen image, and at the same time, the above-mentioned specimen is set. The brightness information related to unnecessary data positions is used as the basis, and the minimum brightness level is set, and then the brightness information is transformed according to the above maximum brightness level and minimum brightness level, and the transformed brightness is used as the basis. Based on the information, it is necessary to determine the background position of the specimen image, the position of the unnecessary data and the position of the specimen image, and record the program position of the extracted specimen image. In the eighth aspect, the recording medium used for microscope image processing includes the color information of the specimen image, and 'identifies the position where the above-mentioned color information is close to the maximum value and the background position of the specimen image, and at the same time, the above-mentioned color information is close to It is a program that records the position of the extracted sample image by identifying the position of the minimum value and the position of unnecessary data of the specimen image, and using the color information as a position above a predetermined threshold value and the position identification of the specimen image *. In the ninth aspect, the recording medium used for microscope image processing is based on the difference in color information at the same position of the specimen image, and then the position where the difference between the color information at the same position of the specimen image becomes the largest is determined. , And set a threshold value based on the difference between the above color information becoming the maximum value, and then use the above threshold value to distinguish the difference between the above color information, and it will be above the threshold value or below the threshold value The program recognizes the position of the specimen image and records the position of the specimen image. Embodiments of the present invention Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a schematic diagram of a microscope image transmission system of the present invention. For example, I __ 's Zhang it this gas 3S furniture (CN-S) A4 see the grid (210 X 297 mm)> 〇I ----- I ------ installation ------ -^ Order -------- line {Please read the notes on the back before filling out this page) 44966 2 A7 --------- B7___ V. Description of the invention (7) In Figure 7F of Figure 1, This microscope image transmission system is such that the personal computer 1 on the observation side and the personal computer 5 on the dependent side are connected to each other via a line connection device (not shown) and a public line (for example, ISDN) 3 that are freely accessible. In addition, each of the computers 1, 5 is provided with monitors 2, 4 for displaying functions for displaying an observation image. The personal computer 5 on the dependent side is connected to the microscope. The microscope 7 is provided with a motorized stage 9 and a microscope 7 related to a motorized rotator 8 and a video camera 6β. Of course, it can also have an unfocused autofocus. The device (unit), dimming mechanism, electric diaphragm mechanism, etc. e are again used in this example, but a microscope equipped with motor power is used, but 'is not limited to this, as long as it also has at least a microscope with a motorized stage, the present invention is applicable invention. Also, in this embodiment, the public line uses ISDN, but it is not limited to this, for example, a satellite line, and it can be applied by a person who can rely on transmitting information. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, the above personal computers 1,5 have various functions including a video capture (not shown) function and a terminal that accepts the video output from the video camera 6. In addition, although the personal computers 1, 5 have an external memory section 31 for storing image information and the like output from the microscope 7, etc., the external memory section 31 is attached to a peripheral device as a peripheral device. 〇 and other recording devices, of course. In addition to the above configuration, a wireless telephone can be connected to the line connection device. According to this wireless telephone, there is no need to hold a microphone, and it is possible to carry out personal computer operation and the like and to talk. In the above example, only the microscope 7 is connected to the personal computer 5. However, it is also possible to connect the Acer photography device to the personal computer 5 9 10 through a video selector and the like. Standard (ΐ_Α4 specifications (il0X 297 public love ·), A7 B7 Printed by the Consumers' Cooperative of the Wisdom and Time Bureau of the Ministry of Economic Affairs. 5. Description of the invention (The second one indicates the function of rebuilding the first one) Box 囷. As shown in FIG. 2, this system uses a microscope 7 and outputs various control signals to the microscope 7 or receives and receives video signals from the photographing section 21 installed in the microscope 7, and will be described later. The processing personal computer 5 is composed of the above-mentioned microscope 7. The microscope 7 is composed of a photographing section 21, a magnification switching section 22, a stage moving section 23, an automatic focusing section 24, and an automatic aperture adjusting section 25. However, the above-mentioned automatic The focusing unit 24 and the auto-tuning unit 25 can be omitted. On the other hand, the personal computer 5 'is formed by the microscope control unit 12, the internal processing unit 13, and the user interface portion 14. The microscope control unit is based on a screen. Memory section 26 , Magnification control unit 27, stage control unit 28 ', focus control unit 29, and variable light control unit 30. The internal processing unit 13 is an external memory unit 31, a specimen area identification unit 32, and a zone. The segmentation section 33 and the information storage section 34 for the relationship between the magnification information and the segment size. The user interface part 14 is composed of the display section 35, the magnification designation section 36, and the segment addition, deletion, and movement designation section 37. The above-mentioned external memory unit 31 is a recording medium of a computer program that is intended to memorize a flowchart described later, such as' hard disk or floppy disk, CD-ROM, MO, etc., and it is also applicable to the second figure. The configuration of the reliance side is mainly described, but the control system of the personal computer 1 on the observation side is excluding the microscope control unit] 2 because it is approximately the same as the control system of the personal computer 5 described above, and is omitted. In the following, referring to the flow chart in Fig. 3, the detailed paper size of the above-mentioned paper will be explained in detail. Applicable to CNS A4 specifications < 210 X 297 male «) ------ installation- ----- Order --------- Line (Please read the note on the back first Please fill in this page again for details) 11 449662 A7 B7 V. Description of the invention (9) Operation sequence of the invented micro sharp image transmission system "First, on the relying side, take the specimen image of the observing side, such as a pathologist, to check As a macro image (step Si), the above macro image is a specimen image taken from a macro photography device (not shown), or a specimen image taken at the lowest magnification of the microscope 7 or an equivalent magnification. The macro image taken by the photography section 21 of the microscope 7 is transmitted through the screen memory section 26 as sequential data through a video capture port (not shown) in the personal computer 5 on the dependent side, and displayed on the display. Screen of the monitor 35 (monitor 4). The above macro image acquisition is started in synchronization with any switching operation. That is, the macro image is taken based on the display 35 on the dependent side (application software on the monitor 4), and the displayed operation SW button is captured by a mouse, or by an external operation (not shown) The disc switch is selected and started. Next, a macro image is taken as described above, that is, the personal computer 5 'on the dependent side makes a line connection request to the personal computer 1 on the observation side (step S2). It transmits the digital information to the personal computer 1 on the observation side through a line connection device (not shown), a public line 3 such as ISDN, etc. The connection request for this line is a transmission line connection request instruction, Initial data, macro image information, etc. The personal computer 1 on the inspection side receives the above-mentioned line connection request instruction, initial data, macro image information, etc. (step S3), that is, the operation right of the microscope 7 is changed on the observation side. The change of the operation right of the microscope 7 may be performed automatically at the time (time) when the line connection is established. In addition, on the screen of the monitor 2 of the display section 35, depending on the application The size of the paper shown in this application is applicable to the standard of the State Council of China (CNS > A4 (210x297 mm)) * Read the note temple on the mouth first? I then fill out this page > Employee Consumer Cooperatives, Intellectual Property Bureau, Ministry of Economic Affairs Print

^ Γ ^ ^ III * -6J ί I-I nnn II 12 A7 B7 V. Description of the invention (10) The button is switched to enable the operator (pathologist) on the observation side to grab the mouse with a suitable time. May be a second, the objective side magnification of the microscope 7 is designated on the observation side (step S4). This magnification designation can be grasped by a mouse on the screen of the monitor 2 of the display section 3 5 of the personal computer 1 on the observation side according to the operation button displayed by the application software, or manually operated on the observation side. The keypad of the operating unit (not shown) of the personal computer 1 can be assigned to a predetermined key. If so, the specified magnification information is suitable for sending to the dependent side of the microscope 7. Next, the inspector (dedicating physician) makes a selection of the network designation or the point designation for the part to be expanded (step S5). Here, the above-mentioned "mesh designation" refers to those who divide a macro image into a grid shape (hereinafter referred to as a section) and designate an enlarged position. On the other hand, "point designation" is a designation of an expanded location with one arbitrary position as the center. Based on the selection of the "mesh designation" or "point designation", the specimen image 'displayed by the monitor (top) of the display unit 35 on the observation side is line-covered, so that the observer (pathologist) can Easy to confirm location. Furthermore, in the present invention, the position information is communicated to the relying side at any time with the mesh designation or point designation of the sharp inspection side. Therefore, the display unit (35) (monitor 4 can also There is the same screen as the observation side. With the above step S5, in the selection of the mesh means, the percentage of the deduction is combined, and the mesh processing is performed (step S6). At the selected point, the flame percentage is combined, and the point processing is performed (step S7) = Among them, it is possible to improve the efficiency of the mesh processing, and the aspect of high precision is one of the features of the present invention. For the mesh processing, a wooden iron sheet will be used.涑 Used by a country building standard (CNSM4 specifications < 210 X 297 male ------------- install-------order --------- line (please (Please read the notes on the back before filling this page) 13 A7 B7 Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Cooperation Du V. Invention Description (11) Various implementation aspects will be described later. Based on the above point processing or net processing Regarding the specified position (area), it is a request to acquire the image at a specified magnification. 5 (step S8). The position information specified above is a recording medium (hereinafter, referred to as a recording medium, etc.) which is recorded in the personal computer 5 on the dependent side and the personal computer 1 on the observation side. It is an information storage device). Then "retrieve the image request from the personal computer 1 on the observation side, and when the personal computer 5 on the dependent side receives the data, the personal computer $ on the dependent side performs the operation of the microscope 7". Into the symmetrical image of the personal computer 1 from the observation side (step S9). That is, in the above-mentioned microscope operation, the position designated by the mesh or point is converted into the position of the stage coordinate, and the stage is driven. The driving unit 23 (motorized stage 9) moves the specimen, and the magnification switching unit 22 (electric rotator 8) is changed in accordance with the designated magnification. Thereafter, the specimen image information is taken by the photographing unit 21. Then, 'the The acquired image information is taken into the video capture port in the personal computer 5 on the dependent side, and the image is converted into a still picture. The image of the still picture is made and recorded in its original state on a hard disk or M0, etc. The external memory device 31 may also be used, but in the present invention, it is convenient for image and image transfer, and it is stored after compressing the image in JPEG format. If so, the image information stored in the external memory device 31, Information transmission is performed from the personal computer 5 on the relying side to the personal computer 1 on the observation side via the line connection device and public line 3 (step Si0) (please read the precautions on the mouth first and then fill out this (Page) ϋ I t I nn IT I Ιϋ 11 n IV n It. This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 14 A7 B7 Employees ’cooperation with the Intellectual Property Bureau of the Ministry of Economic Affairs ii, s · V. Description of the invention (I2) When the image information transmitted from the personal computer 5 on the dependent side is received by the personal computer 1 on the observation side (step s 丨), the personal computer on the observation side] The video information is displayed on the daytime surface of the monitor 2 of the observation-side display portion 35. As mentioned above, when the image information is compressed using JpEG Fang's, etc., that is, when the image is displayed, the predetermined stretch processing is performed at the same time. If all the images have been transferred, a remote observation is performed on the observation side for diagnosis (step S12). At this time, the connection of the image and the position information of the mouse are transferred from the observation-side personal computer with operation right to the personal computer 5 through the line connection device and the public line 3. Receiving the above image link > personal computer 5 on the relying side for information or mouse position information, based on such information, displays the image on the display part 3 5 (monitor 4) on the relying side, and also links the slide Mouse location and more. In general, since the distance diagnosis is performed by the personal computer 1 on the observation side, the operation right is on the observation side, but the operation right can be arbitrarily switched to the personal computer 5 on the dependent side. As described above, after the long-distance observation is performed, a judgment is made as to whether it is finished or not (steps Si4 and S15). Here, if the objective lens is to be enlarged to a high magnification and the diagnosis is to be continued, that is, to return to the above-mentioned step 84 and repeat the above-mentioned series of processing, and if no further examination is to be performed, all the processing is terminated. If there is no final request from the observation side to the relying side, the process returns to step S9 to wait. In the present invention, in the above sequence, the purpose is to improve the mesh processing of step%. That is, the designation of the mesh division after receiving the data of the Acer image should be aimed at achieving the following correctly and easily, and the relevant parties and papers will be used in the National Sector (CNS) specification (210 X 297 meals). -------------- Installation -------- Order · -------- line (please read the precautions of the threat surface before filling out this page) 15 Economy Printed by the Intellectual Property Cooperative of the Ministry of Intellectual Property, 449662 A7 B7 V. Detailed description of the first to seventh implementation aspects of the invention description (13). "First implementation aspect" First, referring to FIG. A series of processing related to the image recognition of the specimen image position according to the microscope image transmission system. The image recognition of the position of the specimen image to be described below is described in the second to sixth embodiments described below. The technology used. At the beginning, when the pathological specimen is photographed with the microscope through the illumination on the relying side, the image shown in Figure 4A can be obtained. In this case, the background portion 51 is white and the specimen image 52 is Dyed, therefore, with color, and the noise part 53 is usually close to black For example, 'prone to occur when taking photographs with a macro image. Occurs when the stage between the copy of the microscope for photography and the clip holding it. Also, other masters may be damaged by dust attached to the optical system. It is identified as the V noise part 53 of the still image. In this embodiment, the image recognition of the position of the specimen image is performed based on the luminance information of the specimen, and if the extracted luminance information is the image, it is given to Genna in On the information storage device (not shown) inside the personal computer 5, 1. On this data storage device, for example, when taking a picture of VGA (640X480), it is given 2 to (640, 480).

The luminance information of the image arranged by the dimension. The image information is divided into three types of information: r, G, and 8; and R (640, 480), G (640, 480), and b (640, 480) are used to provide information for each frame. Among these, it is appropriate to use the information of G (640, 480) whose shape is easier to recognize. The specimen images shown in Figure 4A are arranged as a two-dimensional paper as described above. The paper size applies the Chinese National Standard (CNS) A4 specification (210x 297 public love). 16 (Please read the precautions on the back before filling this page. )

A7 A7 Consumers' Cooperative, Indun, Employees, Smart Time and Industry Bureau, Ministry of Economic Affairs 5. Description of Invention (w)

In the case where Yugner is in the information memory f, the background part 51, MG, and b make the frame information close to F⑽), and the noise part 53, R, G, and B all fall below a certain threshold ( (Close to correction), and the specimen image, if it exists, becomes the data stored in the predetermined range. Here, from the specimen image shown in FIG. 4A, the luminance information of arbitrary γ coordinate (Yi) is extracted, and it becomes as shown in the figure. Based on this characteristic, the specimen image upper limit luminance level 55 and the specimen image lower limit luminance level 56 are set. The upper limit of the luminance level can be set to identify the luminance of the background portion 51. On Gu Zhao, follow the method below. That is, the operator (observation side or dependent side) takes the background portion 51 as a finger towel with the bone mouse, and the brightness information of the image corresponding to the position of the mouse pointer arrow is obtained from the data storage device. Read out and use it as the background luminance level, and set a value slightly lower than this value as the specimen image upper limit luminance level 35. Alternatively, when the specimen image is intended for Gna on the data storage device, a segment with continuous repeat rule, G, B is close to the value of FF (255), and this segment is used as a scene Automatically recognize, and average the frame information of the segment as the background brightness level, and set this value to a slightly lower value as the upper limit brightness level of the specimen image 55, of course can. As for the lower limit brightness level 56 of the specimen image, it is the same as the above-mentioned limit brightness level 55 of the specimen. 'The operator grabs the murmur part 53 with a mouse and instructs it, and the brightness information of the image corresponding to the position of the mouse is indicated. The above data memory device is used to make the grapefruit, and it is used as the noise level, and the value is set to a slightly higher value as the specimen lower limit luminance level 56ls, and the upper and lower limits are both ------- ------ Equipment -------- Order ------- • Line (谙 Read the precautions of the flank first and then fill in this X) Home 埤 m (CNS) A ·! Specification 297 (Mm) 449662 Δ7 B7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs V. Invention Description (15) The operator can change it at will. After the above setting is completed, the brightness level is set to FF (2 55) for all those that are not within the range of the specimen image upper limit luminance level 55 and the specimen image lower limit luminance level 56. As a result of this data transformation, as shown in the figure, only the specimen image has a brightness level of 0. Secondly, the transformed data is used as the basis and the specimen image is displayed again, that is, as shown in Fig. 4D ', only the specimen image is displayed in black. The transformed Zen information is input to the data storage device in a two-dimensional array, and only the frame of the specimen image has a luminance level of 0 and the others become the value of the luminance level FF (255). . In the first embodiment of the present invention, the range of the specimen image is detected by using the above upper limit and lower limit luminance information of the specimen image, and at the same time, it will refer to the previous 2-dimensional array data of Genna in the data storage device, and Perform the specified mesh designation. The following process will be described with reference to the flowchart in FIG. 5 and the example of the display in FIG. 6 in the first embodiment. First, as shown in FIG. 6A, the minimum coordinates (Xmin, Ymin) and the maximum coordinates (Xmax, Ymax) existing in the specimen image are automatically detected based on the luminance information arranged in the above two dimensions (step S21). Next, the width and height of the designated segment are calculated from the designated magnification (step S22). The specified magnification can be determined by grabbing the button Sw displayed by the application software with a mouse, or dividing and assigning a function (function) key such as a keyboard of a personal computer. Regarding the calculation of the degree and height of the above-mentioned designated sections, here, the current magnification of the objective lens is used as the basis (read the precautions of the mouth first and then fill out this page) This paper applies the national standard of 4 * ( CNS) A4 size (210 * 297 mm)

• > 1 i I cl II-Hi K— I-^^ 1 a— n cl I -1 III 1 I 18 Ministry of Economic Affairs% Huishi Industry Bureau MT Industrial and Consumer Cooperation _Social Printing A7 ---- ^ _B7________ 5. Description of the invention (16) The ratio of the exact magnification shall determine the size of the section. That is, when the magnification of the specimen image currently displayed is 2 times, and the next designated magnification is 4 times, the width and height of the designated segment will be half the size of the segment displayed at (640 X 480) ( 320 X 240). If "the size of the designated section is determined, the initial position of the mesh designation is determined as shown in Fig. 6B". That is, the aforementioned minimum coordinate value is set to the upper left of the designated section 'and a section 63 is created (step S23) > The position of the section 63 is stored in the data storage device as an initial position. It is possible to memorize the center position or the upper left and left coordinates. After the initial position is determined, as shown in FIG. 6C, the next mesh position is detected (step S24). That is, the X coordinate is fixed, the initial position of Y movement is used as the position of the height of the previously memorized segment, and the designated segment is moved in the Y coordinate direction, and the place where the specimen image exists is detected. Where at least one specimen on the X-line on the section is identified as having a specimen, the movement in the Y direction is stopped, and the position is used as the next mesh position 64, and the coordinates are stored in the data storage device (step S25). ) °, and then move the segment in the Y direction and detect it as the next mesh position (step S26). Then, as shown in FIG. 6D, if the center coordinate of the segment exceeds Ymax (step S28), the X position is moved only by the length of the width of the first segment 63 (step S29), and this is taken as X start position, and the place where the specimen exists (mesh position 65 to 67) is detected in the same way as the above step S24. ί * '凌 纥 t 屯 a ® 家 棵 AI, C >: S) A4 size (2) 0 X 1! 97 public + meal) 19 ---------- install -------- -Order ----- 1--line < confusion read the notes on the back before filling in this page) 449662 Α7 Β7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 5. Description of the invention (17) Repeat as described above As shown in FIG. 4E, if the center coordinates of the segment exceed (Xmax, Ymax) (steps S28, S30), the final mesh designation is completed. The above-mentioned mesh designation results are displayed as line overlay on the monitor 4 on the relying side and the monitor 2 'on the observing side, and the segment designated by the position taken in together with the specimen image is displayed. The position data of the mesh division on the monitors 2 and 4 is transformed into the coordinate data of the motorized stage 9, and the motorized stage 9 is moved to each mesh based on the image acquisition requirements from the observation side. After dividing the designated position, the microscope 7 is released and a specimen image is taken in the imaging section 21 (video camera function 6). The conversion of the coordinate system on the above-mentioned monitor into the coordinate system of the motorized stage 9 is performed based on a conversion table stored in advance by a data storage device in the personal computer 5. Then, based on the data transferred from the personal computer 5 to the motorized stage 9, the motorized stage 9 is moved. And 'after the movement of the motorized stage 9, the image is taken and the unconnected line connection device and the public line 3 are taken in, and the image is recorded from the personal computer 5 on the dependent side to the personal computer 1 on the observation side. Transfer β As described above, according to the microscope image transmission system related to the first embodiment, with a simple operation, the noise of gray dust and other parts can be removed, and the mesh segmentation can be automatically performed only on the specimen image. "Second embodiment" Hereinafter, a second embodiment of the present invention will be described. In the aforementioned first implementation aspect, the detection of the specimen image position above uses the X coordinate as a reference to determine the designated mesh division limit. However, since the paper size of the specimen applies the Chinese National Standard (CNS) A4 (210 X 297) Love) 20 (Please read the note on the arm surface before filling in this page > \ Λ_

— — — — — — — — I-I 1 IIIII — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — II-III II II .. _ _ B7 V. Description of the Invention (l8) There are also occasions when it is better to specify the mesh segmentation with γ-based enumeration. In consideration of this problem, the author is the second embodiment, and in this embodiment, it is characterized by designating the mesh division as the Y standard. That is, as shown in FIG. 7, the mesh division designation is performed in the order of the mesh positions 71 to 76. "Third embodiment" The third embodiment will now be described with reference to the flowcharts in Figs. 9 and 10 and the example shown in Fig. 8. In this third embodiment, it is possible to designate the mesh ratio of the majority as possible, or even the mesh division of the yield ratio. First, with reference to the flowchart in FIG. 9, the procedure of the extended section designation process according to the third embodiment will be described. In this sequence, the minimum coordinates (Xmin, Ymin) and the maximum coordinates (Xmax, Ymax) of the position where the specimen image exists are automatically detected first (step S31). Next, the width and height of the designated segment are calculated at the designated magnification (step S32). The specified magnification is established by grabbing the displayed button SW based on the application software, or by assigning the magnification to a function key such as a keyboard of a personal computer. The calculation of the width and height of the above-mentioned designated section is performed based on the ratio of the current magnification of the objective lens as the reference. After determining the size of the above-mentioned designated section, the initial position of the mesh designation is determined. That is, the aforementioned minimum coordinate value is set to the upper left of the designated area (step S33). After this setting is completed ', it is judged whether or not a specimen exists at the position where the phase is set in the upper left (step S34 4). In the above step S34, if it is judged that there is a specimen, 'it is calculated 21 ----- install -------- order --------- line (please read the note on the back first) Please fill in this page again for details) 44966 2 Α7 Β7 Printed by the Consumers' Cooperative of Wisdom and Time Bureau of the Ministry of Economic Affairs 5. Description of Invention (19) The ratio of the area of the section to the area of the specimen image in the section (step §3 5). Next, it is judged whether or not the area of the specimen within the area of the section is 50% or less (step S36). Here, if it is judged that the area ratio is less than 50%, that is, the segment processing (intra-segment mesh processing) in the segment can be performed at a higher magnification (step S38). The detailed description will be described in the flowchart of FIG. 10 described later. An unillustrated data storage device that stores the position of the segment as a false coordinate and uses the existing specified magnification before mesh processing in the segment is stored in the personal computer 5 or 1. After the processing of the above-mentioned high-magnification designated section is completed, it returns to the designated magnification and the next mesh position is detected. That is, the γ coordinate is fixed, and the initial X-movement position is moved from the position of the aforementioned pseudo-coordinate to the position in the direction of the X-coordinate in consideration of the width of the segment (step S39). Next, it is judged whether the center X coordinate of the segment after the movement exceeds Xmax (step S41), and if it is not exceeded, it returns to determining whether a specimen exists in the segment after the movement (step S34). On the other hand, in step S36, when the ratio of the area of the segment to the area of the specimen image in the segment exceeds 50%, the enlarged designated segment of the existing designated magnification is displayed as a route, and the coordinates are stored in the data memory. Device (step S37). After the above coordinate recording position is determined, the next mesh position is detected as shown in FIG. That is, the Y coordinate is fixed, and the initial moving position of X is moved in the X coordinate direction to a position that takes into account the width of the previously memorized segment (step S39). {Please read the precautions of the arm surface before filling out this page) This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 22 A7

Consumer cooperation of the Intellectual Property Bureau of the Ministry of Economic Affairs!-! 7: Food V. Invention description (20) Secondly, it is judged whether the center X coordinate of the moved section exceeds the maximum coordinate (Xmax) (step S41). When the maximum coordinate is not exceeded here, it is judged whether a specimen exists at the position of the section after the movement (step S34). In the above step S34, if it is determined that the specimen does not exist at the position of the section after the movement, the Y coordinate is fixed and the section is moved by 1 pixel in the X direction (step S40). This moving process has the same meaning when there is no specimen image at the initial position set on the upper left. Next, it is judged whether the value of the center X coordinate of the section after the section moved by 1 pixel portion exceeds the maximum X coordinate (Xmax) (step riding S4 1). In this case, if it is not exceeded, it is determined whether or not a specimen exists (step S34). The process continues until a specimen exists or the X coordinate of the center of the segment after the segment moves exceeds the maximum X coordinate (Xmax). Then, if the center coordinate of the moved segment exceeds the maximum X coordinate (Xmax) (step S41), the Y position is only given the length of the height of the moved segment, and the X position is set to Xmin (step S42) . The coordinates specified here are the coordinates on the upper left of the segment. When the center Y coordinate of the above-mentioned moved section exceeds the maximum γ coordinate (Ymax) (step S43), it is the end. When the maximum X coordinate is not exceeded 'is to determine the next mesh position, and it is returned to the judgment processing of whether or not a specimen exists in the segment position after the movement (step S34). Next, refer to the flowchart in FIG. 10 '釺 For the case where the area ratio of the specimen image in the section in FIG. 9 is 50%, the section designation processing for the souther magnification designation in the section (the following is performed) (Referred to as the mesh designation in the section) for explanation = ------------ * installation ------- order ----- I --- line f Please read the note on the back first Please fill in this page again} -23-449662 Α7 Β7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of Invention (21) First, determine whether there is the same object in the section that can be specified at a higher magnification (step S51). If the specified magnification of the current section is not the highest magnification, the processing is executed; otherwise, the processing is terminated. When it is judged that the magnification is higher than the designated magnification of the current segment in step S51, the minimum and maximum coordinates (Xmin, Ymin), (Xmax, Ymax) of the specimen image in the segment are detected (step S52). Next, the width and height of the enlarged designated area are calculated at a higher magnification than the designated magnification of the current segment (step S53). For example, if the microscope has an objective lens of 25x, 2x, 4x, 10x, 20x, and 40x, and the magnification of the current segment is specified as 2x, a higher magnification objective is set to 4x, and the value calculated on the segment Higher Width and Height of Enlarged Segment> Then, 'the initial position of the enlargement of the specified segment within the segment is set to the upper left of the segment (step S54). Then, it is determined whether a specimen exists at this initial position (step S55). Here, when a specimen exists, the ratio of the area of the initial segment to the area of the specimen image in the segment is calculated (step S56). Next, it is determined whether the area ratio is 50% or less (step S57). Here, if it is 50% or less, the designated segment processing (the mesh processing in the segment) is performed at a higher magnification (step S59). Mesh processing in the section is performed until the specified objective lens exceeds the maximum magnification. In this example, the segment mesh is continued up to the maximum magnification, but it is also possible to perform mesh processing in the segment with an arbitrary magnification as the upper limit. The current magnification before mesh processing in the segment is used, and the position of the segment is recorded as a false coordinate in the data storage device. When the mesh in the section is processed, the paper size applies the Chinese National Standard (CNS) A4 specification (210x297 public love) 24 (谙 Please read the precautions on the back before filling this page) -nn II ϋ ϋ If 5, n II ϋ n ϋ IIII ϋ If ϋ I ϋ I n / ϋ I ϋ ϋ n I 1 A7 B7 Consumption of employees in the Intellectual Property Bureau of the Ministry of Economic Affairs. Spit. Printed 5. Description of the invention (22) After the end, it will be restored to the specified rate and inspected. Next is the mesh position. That is, "the X coordinate is fixed", and the initial position of the? Movement is taken as the position in which the segment height is taken into consideration from the aforementioned segment position of the false coordinate, and is moved to the? Coordinate (step S60). Then, it is determined whether the center γ coordinate of the segment after the movement exceeds Ymax (step S62), and if not, it is restored to judge whether the specimen of the segment after the movement exists (step S55). On the other hand, if the ratio between the area of the section and the area where the specimen image exists in the section is more than 50%, it will be displayed in the line with a higher magnification in the section, and the coordinates and the designated object at this time will be memorized. Magnification (step S58). In order to detect the mesh position 'in the next segment', X is fixed and moved in the direction of the γ coordinate as the position of the height of the segment after γ's movement is considered (step S60). If the γ coordinate of the center of the segment after the movement does not exceed Yrnax (step S62), that is, it is restored to determine whether a specimen exists in the segment after the movement (step S55). In the above step S55, it is determined whether a specimen exists. As a result, if the specimen does not exist, X is fixed, and the section within the j pixel section is moved in the γ direction (step S61). Then, when the center coordinate Y of the moving section in the section exceeds Ymax (step s62) ', the X position is only given to the width of the moving section in the moving section, and the Y position is set to Ymin (Step S63). Then, it is determined whether the center X coordinate of the moving section in the section exceeds the maximum Xmax (step S64). Here, when the center X coordinate exceeds the maximum xmax, that is, the mesh processing in the section is ended. In addition, if the center X coordinate of the moving section in the section does not exceed the maximum Xmax, it will be restored to the national standard (C \ -S > A4 (210 X 297 mm>) --- I --------- Equipment !! ----- Order -------- line (please read the meaning of Ken Noodle before filling out this page) Member of Intellectual Property Bureau, Ministry of Economic Affairs印 Printed by a consumer cooperative 449662 Α7 __ B7 V. Description of the invention (23) The judgment processing of the existence of the specimen (step S55), and the foregoing processing will be continued until the center coordinates of the moving section exceed Xmax, Ymax. As described above According to the microscope image transmission system related to the third embodiment, a more appropriate mesh segmentation can be performed automatically based on the occupancy ratio of the specimen image in the section. "Fourth embodiment" Next, the invention will be described. Fourth implementation aspect The fourth implementation aspect is a method of identifying the position of the specimen image by using color information as its characteristic. That is, in general, pathological specimens are colored by the red system or stained by the blue system. The color is "but in the fourth embodiment, the specimen was identified because the staining information about the specimen image was detected. In terms of structure, only the second sensor in the spatial frequency domain where red is extracted from grapefruit will be used. The second sensor will only be extracted in the spatial frequency domain where blue will be used, and the third sensor will only be extracted in the green spatial frequency domain. The detector is different from the first embodiment in that it is provided in the microscope 7. In each sensor, the specimen image can be divided and guided by the beam splitter in the video camera 6. Therefore, each sensor The positions of the sensors are arranged at the same positions as those of the video 6 camera. The outputs of the sensors 1 to 3 above are extracted in pixel units, and these are recorded in the personal computer. The data memory device. Also, the position where the output of the first to third sensors is close to the maximum value is recognized as the background position, and the output of the first to third sensors is the position close to the lowest value. 'Identified as an unnecessary data position, and only extracted the red space frequency range and only the output from the first sensor, the position above a certain threshold is identified as the specimen image position. Because of the staining method also Available for this paper only With China National Standard (CNS) A4 specification (210 X 297 mm) 26 ------------- Li ----— -Order --------- line 丨 t : < Please read the notes on the back before filling in this page) A7 A7 Ministry of Economics and Intellectual Property Bureau, 3 Industrial Consumer Cooperatives _______B7__ V. Description of Invention (24) The blue space frequency field is extracted and only from the second The output of the sensor becomes a position above a certain threshold value as the specimen image position. For the identification of the specimen image position according to the color information, in addition to the first to third sensors described above, it is only used in the microscope 7 It is also possible to detect optical lenses that pass through the spatial frequencies of red, green, and blue, respectively. That is, for one personal sensor, the optical filter of the microscope 7 is switched, and the image of the spatial frequency domain for the purpose is guided to the video camera 6 and the specimen image is identified from the obtained image information. Existing location and can also be recorded in the data memory device. In terms of the sensors used in the above embodiments, a two-dimensional area sensor is preferred, but it is not particularly limited. In the above implementation aspect, the first, third, and third sensors are used to view all the frequencies of the red, green, and blue sections. However, the frequency is not limited to this. For example, only the signals corresponding to the more easily recognizable states are used. The green spatial frequency sensor can also recognize the background position 'unwanted data position, specimen position. As described above, 'Transferring Microscope Images Related to the 4th Implementation Aspect' can also perform high-precision mesh segmentation based on the color information of the specimen image. "Fifth Embodiment" Next, a fifth embodiment of the present invention will be described. The first to third embodiments described below are examples of automatic mesh division. In contrast, the fourth embodiment is to automatically divide the mesh division position once determined by the operator. Since the mesh segment displayed on the monitor of the personal computer is operated, it can be arbitrarily moved, deleted and added. Special, 3 with national scale (CNS) A4 regulations (21ϋχ297 mm. ¾ ----------- 1 II --------------- line < please (Please read the note $ on the back of the book before filling out this page) 27 449662

V. Description of the invention (25) Hereinafter, referring to the flow chart in FIG. 11 and the example of the illustration in FIG. 12, the movement operation of the mesh section according to the fifth embodiment will be described. First, among the segments divided by the meshes, any segment to be moved is operated on the screen of the monitor 2 by using a mouse or the like to capture it (step S101). Identify the position coordinates of the mouse, and store the coordinates in the unshown data storage device inside the personal computer 5 or 1 (step S102). Then, the recorded coordinate information of the mesh division is saved by the data storage device. Take out (step s103). Due to the mouse position and mesh coordinate position data, it is determined whether there is a mesh coordinate position at the mouse grabbing position (step S 丨 〇4). The grabbing position of the mouse is In the designated section, that is, it has the judgment function that its area (range) in the hold section can be recognized. When the mesh section already exists in the mouse grabbing position (step S105), It is recognized as the mesh to be moved. The original state of the mouse is to move the mouse (step s 106) and change the display position of the mesh (step S107). The change of the above mesh position is the mesh line Eliminated, The creation is performed repeatedly. The erasing of any place in the mesh division position is to determine whether there is mesh division coordinate information at the mouse grabbing position and perform it according to the deletion device. The deletion device uses the attention button on the keyboard. For example, you can use the "Delete" key, or you can use the delete button indicated by the application software. 28 {Please read the precautions on the arm surface before filling out this page) Printed by the Intellectual Property Bureau's Consumer Cooperatives This paper size applies to China National Standard (CNS) A4 (210 X 297 public love)

-^ 1 ^ 1 ^ 1 ϋ I— ^ -eJ It n I I n n ti n I

V. Explanation of the invention (26) Addition of mesh segmentation position 'is to judge whether there is mesh segmentation coordinate information at the mouse grabbing position. Then, if it does not exist, it may be possible to specify a new mesh section in the new rule, and when the mouse grabs it, the coordinate position of the new ruled mesh is recorded in the data storage device. As mentioned above, once the mesh division designation was performed automatically, the operator could arbitrarily move, add, or delete the mesh division designation. As described above, according to the microscope image transmission system related to the fifth embodiment, after the mesh is automatically divided, the segment can be moved appropriately at the desired position. That is, the degree of freedom (elasticity) of observation can be improved. "Sixth embodiment" Next, a sixth embodiment of the present invention will be described. In the sixth embodiment, it is characterized by automatically recognizing the automatic focus position of the mesh. In the case of designating an image to be taken by mesh division, that is, to fix the focus position to one place and take in a large number of mesh division positions, the following description will be described with reference to the flowchart in FIG. 13 and the representation example in FIG. 14. The sixth embodiment performs an automatic focus position recognition operation. When the focus position is determined based on the image's light-dark ratio information ’The larger the light-dark difference is, the more accurate auto-focus can be made. When the mesh segmentation position is detected automatically, "First, the mesh segmentation position information with the specimen image near the center coordinates" and the maximum brightness level difference in each section are used to indicate the number of points within the personal computer 5 or 1. Pictured data recording device. Whether or not a specimen image exists near the center coordinates can be determined by reading the aforementioned specimen image identification data from a data storage device and using it. ------------- Install ------- Order · -------- Line (Please read the Note on the back? Matters before filling out this page) 29 449662 Economy Ministry of Intellectual Property Bureau employee cooperation prints A7 B7 V. Invention Description (27) That is, in the case of VGA display 'g (320, 240) as the center, and can be from any frame segment (such as '50 pictures Frame X50 frame) to determine whether the luminance information is 0 (specimen exists) (step S51). This section can also use the averaged luminance information. "Select the section with specimen images near the center of the image 'for the purpose of automatic focusing. The line sensor for the automatic focusing of the microscope must be near the center. The reason for the information. Pomelo comes out of the mesh section where the specimen image exists near the center of the image, and the location of this section is on the data memory device. Next, the segment with the largest luminance difference is extracted from all the segments of the mesh where the specimen image exists near the center of the image (step S52). Then, the mesh division position having the largest luminance difference is detected, and this position is stored in the data storage device as a place to be automatically adjusted (step S53). In this embodiment mode, the mesh decomposition position ι〇1 is selected as a place for automatic focusing. Actually, when the mesh-divided image is acquired, first, the motorized stage 9 is moved to the place where the automatic focusing is performed, and controlled by the personal computer 5 side so that the microscope 7 is automatically performed at the position. Focus control. As described above, the microscope image transmission system 'according to the sixth embodiment is based on the results of mesh segmentation, and with a simple operation, it can automatically identify the focus position with high accuracy. "Seventh embodiment" Next, a seventh embodiment of the present invention will be described. Generally, a macro image will have insufficient peripheral light (see Figure 15), and the insufficient peripheral light will reduce the brightness level. Therefore, the Chinese standard (CNS) A4 specification (210x297 male «) is applicable to the standard paper size. 30 (Please read the precautions on the 't side before filling out this page) -n ϋ ϋ ^ eJI ϋ u I n I n I— nn If n ϋ nn _ A7 — B7 V. Invention description (28) The automatic mesh segmentation position recognition algorithm is based on the luminance information. I_______I____install I — (Please read the attention of the arm surface first.) Fill in this page for the reason. Therefore, due to the influence of insufficient ambient light, you may not be able to specify the correct mesh division. That is, when insufficient ambient light occurs, the information that should be identified as the background may be misidentified. It is the brightness level of the specimen image, so the mesh segmentation position is also covered on the background. In the seventh implementation aspect, the proper mesh segmentation designation is performed on the specimen image where the peripheral light quantity is insufficient. The following features With reference to the flowchart in Fig. 16, the detailed description of the mesh division designation of the specimen with insufficient peripheral light quantity will be described.-The line first "actually recognizes the specimen image as data, The state of the specimen is taken into the image, and the image information is recorded in a data storage device or a recording device such as M0. In this embodiment, ^ 0 (640, 48〇), GO (640, 480), BO ( 640, 480) of the two-dimensional permutation data is given to Genna in the data storage device (step S61). In this case, because of the background information of the photography, 'the image becomes white, and the luminance information of all pixels should be FF (255) However, the position of coordinates in the surrounding part will not become ff (255). The staff of Taiwan Bureau of the Ministry of Economic Affairs will cooperate with consumer spending. Tu-. ^ Therefore, when it is arranged in the 2nd dimension, it will be performed for all pixel information. The calculation is as follows: R0 (x, y) = FF (255) -R0 (x, y) (x == 1 ~ 640, y = 1 ~ 480) G0 (x, y) = FF (255) — G0 ( x, y) (x = 1 to 640 'y = 1 to 480) 31 449662 A7 B7 V. Description of the invention (29) BO (x, y) = FF (255) -B0 (x, y) (x = 1 (~ 640, y = 1 to 480) That is, the luminance information that should be added to the pixel portion with insufficient peripheral light quantity is stored in R0 (640, 480), G (640, 480), B (640, 480). Then 'take the original image of the macro image' and the image information It is stored in the 2-dimensional array memory of R1 (640, 480), Gl (640, 480), and Bl (640, 480) (step S62). The image information of the original image is added to the image information without the specimen image and The data is R (640,480), G (640'480), B (640,480). Here, each image is added (step S63). The details are: R (x, y) = Rl (x, y) + R0 (x, y) (x = 1 to 640, y = 1 to 480) G (x, y) = Gl (x, y) + G0 (x, y) (x = I to 640, y = 1 to 480) B (x, y) = Bl (x, y) + B0 (x, y) (x = 1 to 640, y = 1 to 480) As described above, according to the microscope image transmission system related to the seventh embodiment, the data can be repaired only for the insufficient part of the surrounding light. That is, in the seventh embodiment, based on the result of repairing the data with insufficient peripheral light quantity as the basis, and performing the aforementioned mesh segmentation designation, a correct mesh segmentation designation can be made automatically. "Eighth Implementation Aspect" Next, the eighth implementation aspect of the present invention will be described. The eighth implementation aspect is to extract excellent information and use the information to detect the specimen image position for its paper size. Applicable to China Standard (CNS > A4 specification (210 X 297 mm) (Please read the precautions on the back before filling out this page) Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs for consumer cooperation

I · J n. ^ 1 n I f 1_ nn * 1 MmM. T— n I 32 Staff Consumption Cooperative of Intellectual Property Bureau, Ministry of Economic Affairs, India A7 ______ Β7 ________ 5. Description of the invention (3〇) Microscope image transmission system . Here, FIG. 17 is a schematic diagram showing the process of detecting the position of the specimen image based on the excellent information in this embodiment, and FIG. 18 is a functional block diagram of the microscope image transmission system related to this embodiment. . Figures 9 and 20 are flowcharts for explaining the operation of the microscope image transmission system related to this embodiment. As shown in Fig. 18, the microscope image transmission system can be largely divided into an image pickup section 500, an internal processing section 501, a user operator 502, and a microscope 503. A video camera unit 20 j and a video rate capturing unit 202 are set in the image acquisition unit 500. The microscope 503 is provided with an auto-focusing section 203, a magnification switching section 204, and a stage moving section 205. In the above-mentioned internal processing unit 5 01 ′, there is an image display memory 206 equipped with a memory to display images, a video capture controller 207 to control the video capture unit 202, a specimen image position recognition processing unit 214, and a memory 300. , The external memory memory control unit 214 to control the external memory 218 ′, to control the focus control unit 215 of the automatic focusing unit 203, to control the magnification control unit 216 of the magnification switching unit 204, and to control the load A stage control unit 217 of the stage moving unit 205. In addition, the memory 300 includes each of the brightness memories 209 to 211, the RG brightness difference memory 212, the BG brightness difference memory 213, the RG brightness difference maximum memory 225, the BG brightness difference maximum memory 226, RG threshold memory 227 'BG threshold memory; various fields of! 28. In the above-mentioned user operation section 502, it is set to display and display S due to family gt (" cN-S > Al specification (210x297 male S)! ^ ~~ " ---------- --- install -------- order --------- line (please read the precautions on -f first and then fill out this page) printed by the Employees' Cooperative of Intellectual Property Bureau of the Ministry of Economic Affairs 449662 Α7 __B7 V. Description of the invention (η) The image display section 219 of the image in the image display memory 206 and the specimen image position identification and designation section 220 * The image storage / call instruction section specifies the focus control focus by the focus control section 215 The designation unit 222, the magnification designation unit 223 designated by the aforementioned magnification control unit 216, and the stage position designation unit 224 designated by the aforementioned stage control unit 217 to control the stage control. The following description of The process of detecting the position of the sample image that constitutes the realization. In this embodiment, 'the sample image under the microscope is taken through the video camera function unit 201, and converted into digital data by the video rate capture unit 202' The digital data is taken into the image display memory vessel 206 as a still The image information is displayed on the image display unit 219. The specimen image is shown in Fig. 17A, and the display example shown in Fig. 17A 圊 is provided on the glass of the microscope where the specimen image is stored outside the area of the specimen image. Covers the area of shadows, dust, etc. The brightness information 'as shown by color separation' at the arbitrary γ-coordinate position of the specimen image of 17A 囷 is shown in Fig. 17B. Background section And noise, the changes in the red and green luminance information are presumed to be the same, and where the specimen exists, that is, the difference between the red and green luminance information. In general, the pathological specimen image is used for Observation and various staining, so the position of the specimen image can be calculated using the brightness difference. In the 17th example, a pathological specimen stained with a red body as a whole is taken as an object, but it is characterized by staining The color information is different β. Therefore, three kinds of information of red and green 'blue are obtained for each luminance information. (Please read the precautions of the threat surface before filling out this page) -I inn 』SJI .1 nn ϋ IIII _ ϋ I [ϋ n I * n

Consumers ’cooperation with the Intellectual Property Bureau of the Ministry of Economic Affairs for printing and distribution Α7 Β7 V. Invention Description (32) Take the difference between the above red and green luminance information, and the displayed form is as shown in Figure 17C. The maximum value Rgref of the luminance difference between red and green is calculated based on the characteristics of the figure, and the threshold is set based on it. The case where the data with the threshold value of the lower X-coordinate value is taken as FFh, and the data with the value above the threshold is 0 is shown in Fig. 17D. If it is the result of calculation at all Y coordinates, and the one shown is the figure 17E. In the above example, it is an example that uses the information of the difference between red and green to detect the image position of the specimen. However, because Of course, the specimen can also detect the position of the specimen image based on the blue and red luminance difference information, or by combining the red and green luminance differences and the blue and green luminance differences. Hereinafter, referring to the flowcharts of FIGS. 19 and 20, the operation for identifying the position of the specimen image using the above-mentioned luminance difference information will be described. First, the red, green and blue (hereinafter, referred to as RGB) luminance information of the entire image of the specimen is stored in each luminance memory 209, 21, and 211 (step S301). Here, the luminance information of red (R) is set as a rule, such as 乂, γ), the luminance information of green (G) is Gdata (X, Y), and the luminance information of blue (B) is Bdata (X, Y). The 2-dimensional permutation is given to Gurner in each luminance memory 209 to 211. X is in the range of 0 ~ (X pixel number "), and ya is in the range of 0 ~ (γ pixel number- 丨)" Second, the brightness difference information on the same pixel will be equal to the difference in luminance δ Membrane 2 丨 2, 213 (step spine S302). That is, take the difference between the luminance information of R and G (RGref (X 'γ)), and give it to the RG luminance difference memory 2 丨 2, and take B The difference in luminance information between G and G (BGref (x, γ)), and Yugner broke the Western standard (CNS> A_J specification (CNS> A_J specifications) in the widow Zhang H's legacy; Μχχ 29Γ public issue 35 ---- --------- Installation ---------- Order * JI I ----- (谙 Read the precautions on the back before filling in this page) 449662 A7 B7 V. Description of the invention (33) BG brightness difference memory 213. (Please read the precautions on the front of the product before filling in this page) RGref (X ♦ Y) = Rdata (X-Y) -Gdata (X > Y) RGref (X * Y) = Rdata (X * Y) -Gdata (X-Y) Next, the maximum value of each luminance difference (RGrefmax, BGrefmax) is detected, and the Gurner is stored in the RG luminance difference maximum memory 225 and the BG luminance difference maximum memory 226. (Step S303) »The threshold value for identifying the specimen image is determined by the maximum value of the luminance difference The RG threshold memory 227 and the BG threshold memory 228 (step S304) »Here, referring to the flowchart of FIG. 20, the order of setting the above thresholds will be described in detail. The maximum value of each luminance difference (RGrefmax, BGrefmax) is retrieved from the RG luminance difference maximum memory 225 and the BG luminance difference maximum memory 226 (step S401), and the specimen staining type is selected (step S402) Then, set the threshold value (RGrefth, BGrefth) according to the type of specimen staining (step S403). RGrefth = RGrefmax / n BGrefth = RGrefmax / η This is printed here by the employee ’s cooperative of the Intellectual Property Bureau of the Ministry of Economy, η, m The value varies depending on the type of specimen staining. Of course, it can be changed arbitrarily. For example, let n == m = 2 and set the half of the maximum value (RGrefmax) of the luminance difference between R and G, and The half of the maximum value of the difference in luminance between B and G (BGrefmax) can also be used as the thresholds RGrefth and BGrefth. Also, according to the dyeing method, there are also specimens with blue subjects. In this case, the values of η and m are set to η = RGrefman, m = 2. When n = 1, that is, RGrefth = RGr efmax is the threshold of the information based on the difference in brightness between R and G. The paper size applies the Chinese National Standard (CNS) A4 (210 X 297 cm). 36

Wisdom and Industry Bureau of the Ministry of Economic Affairs 3'Working and Consumption Combination 'ti.5VVY A7 B7 V. Description of the invention (34) The value setting is increased to the maximum level. Because of this, it is equal to detecting the specimen image position a only with the threshold setting of the difference in luminance between B and G, and it returns to the description of FIG. 19 again. As described above, after the threshold values (RGrefth, BGrefth) are determined, the area where the specimen image is to be identified is determined (step S305) »Where the position of the entire image of the specimen is to be identified, it is Xmin = 0 'Xmax = ( X painting prime number -1), Ymin = 0, ΥΓη3χ = (Υpainting prime number -1) c It is also possible to detect a part of the whole image from the whole picture because it determines the field. When the area to be tested is determined, it is judged whether a specimen image exists in the area. First, an initial value (X = Xtnin 'Ymin) of the specimen image recognition detection to be performed is set (step S306). After setting the initial value as described above, each luminance difference data (RGref, BGref) is compared with a threshold value (RGref, BGref) for specimen image recognition, and the presence of a specimen image is detected. First, the red-green luminance difference data (RGref (X, Y)) described above at an arbitrary X'Y coordinate is compared with the red-green luminance difference threshold value (RGrefth) (step S307). Here, if RGref (X, Y) > RGrefth, that is, there is a specimen image on the (X, γ) coordinate (step S3 10). And 'if RGref (X, Y) S RGrefth, that is, the specimen image recognition detection is performed due to the blue-green luminance difference (step S308). Here, RGref (X, Y) > RGrefth, that is, 'there is a specimen image present at (X, γ) coordinates (step S3 1〇)' Conversely, if RGref (X, Y) SBGrefth, that is, in There is no specimen image on (X, γ) coordinates (step S309) ° If so, at any (X, γ) coordinates I -II n I. H ί ϋ ί a 5, »ϋ ϋ ϋ n 1« t (Please (Read the precautions on the back before filling this page) 37 449662 A7 B7 V. The specimen image detection on the description of invention (35) is over. Therefore, the specimen image detection of the second coordinate will be performed. (X = X + 1) (step S311), and it is detected whether the new X-coordinate value exceeds the maximum value (Xmax) (step S312). If the X coordinate exceeds the maximum value (Xmax) ', it returns to the process of detecting the existence of the specimen image on the coordinate (step S307). If the Y-coordinate exceeds the maximum value (Ymax), that is to say, “All the specimen images have been identified and detected, and it will end. "Ninth Embodiment" Next, a ninth embodiment of the present invention will be described. In the ninth embodiment, after the mesh expansion limit designation is automatically designated, among the above-mentioned mesh expansion designation limits, a person who automatically detects the position of the mesh expansion limit that was originally designated for auto-focusing is automatically detected. First, Fig. 21 shows how the entire specimen image is automatically designated by the mesh expansion limit. In FIG. 21, the width of the individual mesh expansion designation limit is set to Frame Width, the height is Frame Height, and the center coordinates of the individual mesh expansion designation limit are (X〇c, Y〇c) to (X3c , Y3c) > The vertex of the coordinates is the upper left of the figure. Hereinafter, referring to the flowchart of FIG. 22, the sequence of setting the automatic focus will be performed first after the entire image of the specimen is automatically expanded to specify the limit. First, the number of mesh expansion designated meshes (Mesh Num) is detected (step S601). Next, in order to hold the individual image data of the specimen image for specifying the limit of the mesh expansion, the specimen image presence detection counter is set as the arrangement data (ChkCounter (O) ~ ChkCounter (Mesh Num-1)) (Step Paper & Degree applies to China National Standard (CNS) A4 specification (210 x 297 mm) -38-(谙 Please read the notes on the back before filling this page) Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Consumption Cooperatives eJ n I nn III n I nnnn 1 nnn It nn It n II—. 4 bureaus of the Ministry of Economic Affairs ar Ηconsumption combination iifr7, '«A7 B7 V. Description of the invention (36) S602). In addition, the presence of individual specimen images for which the mesh is enlarged and specified is detected, and the pixel number data identified as the specimen images are added to (ChkCounter (O) ~ ChkCounter (Mesh Num-1)) (step S603). Here, with reference to the flowchart of FIG. 23, a detailed procedure for detecting the pixel number data in which a specimen image exists will be described. First, set the thresholds (BGrefth, BGrefth) for recognizing the specimen image, and store the icon memory in the personal computer at the end of the reliance (step S701). The threshold setting is as described above. Then, an initial value is set to judge whether or not the coordinates of the specimen image exist (step S702). Here, Xic and Yic are equivalent to (X〇c, YOc) ~ (X3c, Y3c) ° Figure 21 Frame Width and Frame Height are the width and height of the designated boundaries of the mesh expansion. Next, after determining the initial value of the coordinates, a detection counter for judging whether or not a specimen image exists is eliminated (step S703). Secondly, change the XY coordinates to obtain the luminance difference information (RGref (X, Y), BGref (X'Y)) on individual celestial elements, and compare the luminance difference information with the luminance difference determined by step S701 above. Limit values (RGrefth, BGrefth) (step S704). Here, "if the luminance difference thresholds (RGrefth, BGrefth) are larger", the detection counter is incremented (step S705). Then, the X coordinate is incremented to prepare a next coordinate (step S706). On the other hand, if the X coordinate is in the area within the designated range of the mesh expansion (step S707), that is, the Y coordinate is incremented and the X coordinate is restored to the initial value (step S708). Then, it is detected whether or not the Y coordinate is outside the jaw area within the designated limit of mesh expansion (step S709). Moreover, if it is in the field, it will be restored to the above-mentioned Tongjia by the State S family (C'NSm4 砚 格 (2.U) X 297 mm ------------ install- ------ Order --------- line {Please read the notes on the back before filling out this page) 39 49 6 6 fc Α7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs ~~- --- B7____ V. Description of the invention (37) Step S704. If it is "when specimen images exist on the individual mesh expansion designation limit", then the number of identified pixels is counted. "If the γ coordinate is outside the area within the range of the mesh expansion designation limit (step S709) That is, on all the Y coordinates within the designated range of the mesh expansion, the number of pixels identified as the specimen image has been obtained, and the number of pixels identified as the specimen image is added to ChkCounter's order as data (step S710). Return to the explanation on lap 22. The number of pixels identified as the specimen image is stored in ChkCounter (1). Here, (I) indicates an arbitrary mesh expansion designation limit number (equivalent to 0 to 3 in Fig. 21). When all the specimen images whose meshes expand the designated limit are detected, the specimen images are sorted in order from the specimen image that has more counters for detection. The sequence of this arrangement is memorized as another variable (step iS604) »Second, from the specimen image presence detection counter with a large value, it is judged in order whether a specimen exists near the center (step S605). If it is determined that the specimen image does not exist near the center (step S606), that is, return to the above step S605 again, and then take out a mesh with a large number of specimen image presence detection counters and expand the designated limit, and judge that it is at the center. Is there a specimen image nearby. Here, when a specimen image exists near the center, it is memorized as the position where AF was originally performed (step S607). As mentioned above, from the expanded designation limit of most meshes, the number of pixels that can best be used as the specimen image can be automatically identified, and there will be a spot where the specimen image exists near the center of the original AF position. This paper size applies to China Standards (CNS) A4 specifications < 210x297 male *)-40 < Jing first read the precautions on the back before filling this page)

-n n I w -------- order -line! > i ——- -------------- Printed by the Consumers 'Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Description of Invention (38) Above' Describe the implementation of the present invention However, the present invention is not limited to this, and certainly does not deviate from the spirit of its subject. Various improvements or changes can be made. For example, in the above embodiment, the starting point of the mesh segmentation is the upper left of the still image and the ending It is the bottom right, but, of course, any position in the still image can be used as the starting point and ending point. As detailed above, according to the present invention, in the long-distance observation of the microscope image, the designation related to the mesh division of the observation field in the initial observation image can be provided efficiently and correctly by simple operations. Microscope image transmission system, microscope image processing method, and recording medium used for microscope image processing. Brief description of the formula: The first circle shows the outline of the structure of the microscope image transmission system of the present invention. * The second circle shows a square circle which embodies a part of the structure of the first circle. The third circle shows this The operation flow of the invention's microscope image transmission system. The fourth (A) to (D) circle is a series of processes for image recognition of the specimen image position by the microscope image transmission system related to the first embodiment. Figure 5 illustrates the first embodiment The flowchart of the process of making the mesh data of the relevant microscope image transmission system β Figures 6 (A) to (E) illustrate the process of making mesh data by the microscope image transmission system related to the first embodiment. The size of this paper is suitable for the standard of S house (CNS > A4 size (210 X 297 mm) 41 ------------ Shang ------- Order -------- --- line (please read the precautions on the back before filling this page) 449662 A7 Β7 V. Description of the invention (39) The explanation diagram of the 7th department about the process of making mesh data with the microscope image transmission system of the second embodiment Fig. 8 is an explanatory diagram of the process of generating mesh data by the microscope image transmission system related to the third embodiment. Fig. 9 is an explanation of the process of generating mesh data by the microscope image transmission system related to the third embodiment. The flow chart in Figure 10 illustrates the case where the area ratio of the specimen image in the section used in Figure 9 is less than 50%, and the section is designated at a higher magnification (hereinafter, referred to as the section). The flow chart of the mesh designation in the paragraph. Figure 11 is a flow chart illustrating the operation of moving the mesh limit by the microscope image transmission system related to the fifth embodiment. Figure 12 is related to the fifth embodiment. Schematic diagram of the operation of moving the mesh boundary of the microscope image transmission system》 Part 1 Fig. 3 is a flowchart illustrating the automatic focusing position identification operation performed by a microscope image transmission system having an M in the sixth embodiment. Fig. 14 illustrates automatic adjustment by a microscope image transmission system in the sixth embodiment. Figure 15 is used to identify the focus position. Figure 15 shows the side where a macro image with insufficient ambient light is generated. Printed by a member of the Intellectual Property Bureau of the Ministry of Economic Affairs and Consumer Cooperatives. Figure 16 is a detailed description of the seventh mode of implementation corresponding to insufficient ambient light. Flow chart of the operation of the related microscope image transmission system for the designation of mesh segmentation * Figures 17 (A) to (E) show the mode of the eighth embodiment, the process of detecting the image position of the specimen due to the extraction of color information Figure 18. Figure 18 is a microscope image transmission system related to the eighth embodiment 42 Please read the note on the arm surface before filling in this page> This paper is again applicable to the national standard (CNS) A4 specification (210 X 297 mm) A7 ----- B7_______ V. Functional block of invention description (40) 4 Figure 19 is a flowchart for explaining the operation of the microscope image transmission system related to the eighth embodiment. Fig. 20 is a flowchart for explaining the operation of the microscope image transmission system related to the eighth embodiment. Fig. 2 丨 shows the detailed description of the microscope image material system related to the ninth embodiment to automatically After the specimen has been expanded with the specified mesh limit all the time, the flow chart of the order of automatic setting will be performed at the beginning of the automatic focusing. Figure 22 is a detailed explanation of the microscope image transmission system related to the ninth embodiment. A flowchart of the order in which the auto-focusing will be performed initially after the mesh enlargement of the entire specimen image is automatically specified. Fig. 23 is a flowchart illustrating a detailed procedure for detecting the pixel number data existing in the specimen image by the microscope image transmission system related to the ninth embodiment. I--H — — — — — — — ^ il — ί — ------ I (Please read the precautions on the back before filling out this page) 105- ¥ I Regulation 4 AS) -C * 'f; If. • ί. ^ -¾ a,' " --- ί 43 449662 A7 B7 V. Description of the invention (41 component numbers are compared with employees of the Intellectual Property Bureau of the Ministry of Economic Affairs The paper size of the printed sheet of the consumer cooperative is applicable to the Chinese national standard (cns) A4 x 297 51 52 53 55 1 ... the personal computer on the observation side 2, 4 ... monitor 3 ... the personal computer 6 on the dependent side 6 ... Video camera 7 '503 ... microscope 8 ... motorized rotator 9 ... motorized stage 12 ... microscope console 13,501 ... internal processing unit 14 ... user interface 21 ... photographing unit 22'204 ... magnification switching units 23,205 ... the stage moving section 24,203 ... the auto focus section 25 ... the automatic adjustment of the aperture section 26 ... the screen memory section 27,216 "· magnification control section 28 ... the stage control section 29, 2 15 ... the focus control section 30 ... Variable aperture control section 31 ... external memory section 32; specimen area identification section 33 ... section division section 34 ... relational information memory Part 35 ... Display part 36,223 ... Magnification designation part 3 7 ... Zone, Xiao Yu, Scale shifting background part specimen like noise part specimen like upper limit brightness level 56 ... specimen lower limit brightness level 63 ... section 64 to 67, 71 to 76 ... Mesh position 201 ... Video camera unit 201 ... Video capture unit 206 ... Video display memory 2 07_ " Video capture control unit 2 0 8 ... Specimen image position discrimination subtraction unit 209 ~ 211 ... intensity memory 212 ... RG intensity memory 213 ... BG intensity memory 214 ... specimen position recognition control unit 44 (please read the precautions of the mouth surface before filling this page) rk------ -I --- I -----. Ί --- III — I______________ A7 B7 V. Description of the invention U2) 217 ·· * Stage control unit 2.1 8 ··· External memory 219 ... Image display unit 2 2 0… specimen position recognition designation unit 22 image storage / readout designation unit 222… focus designation unit 224… stage position designation unit 2 2 5… RG brightness difference maximum value memory 2 2 6… BG brightness difference Maximum memory 227 " .RG threshold memory 228—BG threshold memory 300 ... memory 500 ... images Entry section 502 ... User operation section ------------- install * — (Please read the precautions on the back before filling this page> Order --------- Wire Economy Department Wisdom Property Consumption of Employees of the Property Bureau ίΐ · ί1κ3ί: ϊ1 45-

Claims (1)

Party 08 449662 6. Scope of patent application [— a microscope image transmission system, which is designated by the sector unit specified in the field of observation of the specimen image magnification 1 = (Jing Xian "Read the precautions on the back and then fill out this page) Camera ( Photo) device (6, 21, 201), which is used to photograph the specimen to obtain the above-mentioned specimen image; recording device (], 5, 26, 31, 202, 206), which is a record containing the specimen photographed by the above-mentioned imaging device Still images of the image: the display device (2, 4, 35, 219) displays the still images recorded by the recording device; and the control device (I, 5, 13, 501) 'is the position where the specimen image is extracted and Based on it, only the area in which the specimen image exists is divided by a predetermined segment unit, and the index indicating the segment is superimposed on the still image and has a function of being displayed on the display device. 2. If the microscope image transmission system according to item 1 of the scope of patent application, in order to extract the position of the specimen image, the control device (丨, 5, 13, 50) is functionally equipped with: Intellectual Property Bureau of the Ministry of Economic Affairs The function of setting the brightness level by the employee consumer cooperative is to extract the brightness information of the still image and set the maximum brightness level based on the brightness information related to the background position of the specimen image, and based on the specimen image. The minimum brightness level is set for the brightness information related to the unnecessary data position; the conversion function is based on the above maximum brightness level and the minimum brightness level to transform the above brightness information; and 46 paper standards are in accordance with Chinese national standards (CNS) A4 specification (210 X 297 gong) A8B8C8D8 Printed by the Consumers' Cooperative of Intellectual Property Bureau of the Ministry of Economic Affairs 6. The scope of the patent application judgment function is to judge the background position of the specimen image based on the above-mentioned luminance information transformed. Data location, location of specimen image. 3. For the microscope image transmission system in the first scope of the patent application, in order to extract the position of the specimen image, the control device (1, 5, 13, 3, 501) still has the function: identification function, the specimen is extracted The position of the color information close to the maximum value is identified as the background position of the specimen image, and the position of the color information close to the minimum value is identified as the unnecessary data position of the specimen image, and the color The position where the information becomes higher than the predetermined threshold is identified as the position of the specimen image. 4. The microscope image transmission system according to item 1 of the scope of patent application, wherein the control device (1, 5 '13' 501) further has a function: a display function, which is located at the initial segment position of the still image setting 'and Using this initial segment position as a starting point, a sequential segment is set in the first direction, and when the segment position exceeds the maximum coordinate, only the segment width is shifted in the second direction, and the segment to be updated is updated. In the first direction, they are sequentially set, and the indicators related to the segment are displayed on the display device. 5. For the microscope image transmission system of the scope of application for patent item I, in order to extract the position of the specimen image, the control device (1, 5, 13, 501) is further equipped with a function: a difference function is to extract the above The color information of the specimen image is based on the difference between the color information at the above position of the specimen image; This paper size applies the Chinese National Standard (CNS) A4 specification (21CU 297 mm) ------ I --- I --- 1 --- Order · ------- < Please read the notes on the back before filling out this page) 47 49662 A8B8C8D8 Sixth Intellectual Property Bureau of the Ministry of Economic Affairs Employee Consumer Cooperatives printed and applied for patents The function of range finding position is to find the position where the difference of the above color information becomes the largest: Set a predetermined threshold value function to set the predetermined threshold based on the value of the difference of the above color information being the maximum; and identify The function is to distinguish the difference of the above-mentioned color information by the above-mentioned threshold value, and to identify the position above or below the threshold value as the position of the specimen image. 6. The microscope image transmission system according to item 5 of the scope of patent application, wherein the function to set the threshold value set above has: a detection function that detects the maximum value of the brightness difference between red and green; The function is to detect the maximum value of the brightness difference between blue and green; and the threshold value function is to take the quotient of the maximum value of the brightness difference as described above and use this value as the threshold value. 7_ The microscope image transmission system according to item 1 of the scope of patent application, further comprising: an operation instruction device (37), which instructs addition, deletion, and movement of a section set by the control device; and Position information related to sections added, deleted, and moved according to the instructions of the operation instruction device is recorded in the recording device at any time. 8. If the microscope image transmission system of item 1 of the scope of patent application, it is further provided with: a focus adjustment (adjustment) device (29, 215), which is detected from the maximum brightness level difference in each area, And compare the largest paper size of each section (China @ 家 标准 （CNS) A4 Specifications） 〇〇 Norwegian f (Please read the precautions on the back before filling this page) Order · Line 48 —D8 'Ministry of Economy Printed by the Intellectual Property Bureau employee consumer cooperative 6. The scope of the patent application and the standard deviation are detected, and the position coordinates of the section with the maximum brightness standard deviation among each section are detected, and this section is used as the focus adjustment benchmark Position and focus adjustment. 9. A microscope image transmission system, which is in the specimen image where the area to be observed at a high magnification is designated, designated by a predetermined section unit, equipped with a photographing device (6, 21, 201), which is a photographic specimen to obtain the above Specimen image; recording device (1'5'26, 31, 202, 206), which records still images containing specimen images photographed by the above-mentioned photographing device; display device (2, 4, 35, 219), which is at least also The still image recorded on the above recording device is displayed; the detection device 'takes a still image without the above specimen, and takes a still image while the above specimen is present, and uses the luminance information of both Based on the difference, the part with insufficient light quantity is detected »The control device (1, 5, 13, 501) excludes the part with insufficient light quantity detected by the detection device, and then extracts it from the still image The position of the specimen image 'and only the area where the specimen image exists' are divided by a predetermined section unit, and the indicators related to the section are superimposed on the above still image and controlled to be displayed on it Display means. 1 〇. — A microscope image transmission system, at least one of which has a control device on the observation side and the dependent side, connected for free communication with each other, and this paper standard applies the National Standard (CNS) A4 specification (210 X 297) Public love) -------- Outfit ----- ί — Order -------- line (please read the note on the back first and then fill in this page) 49 449662 Ira 6. Scope of Patent Application Among the specimen images, the area to be observed at a high magnification is divided by a predetermined section unit. The above-mentioned dependent side has: a photographing device (6) for photographing the specimen to obtain the specimen image; a first display device ( 4) 'It displays a still image containing a specimen image photographed by the above-mentioned photographing device; and the first transmitting and receiving device (3, 5) is for transmitting and receiving information with the above observation side; and the above observation side has: The second display device (2) 'displays still images transmitted from the relying side; and the second transmitting and receiving device (1, 3) sends and receives information from the relying side: Intellectual Property Bureau, Ministry of Economic Affairs Printed by an employee consumer cooperative and the above control device has The display function is based on the position where the specimen image is extracted from the still image displayed by the display device using the control device, and based on it, only the area where the specimen image exists is divided into predetermined sector units. 'The indicator indicating the segment is displayed on the still image superimposed; the transmission function is also to target the still image of the display device not using the above control device and the still image displayed by the display device of the above control device. The image is at least a function of sending position information indicating the index of the above-mentioned section to the unused side by using the control device side; and the same information is shared by the relying side and the observation side. — II ^ -------- ^ ------ 1! ^ (Please read the notes on the back before filling out this page) This paper size applies to China National Standard (CNS) A4 (210 X 297) Mm) 50 Zhongjing Patent Scope Ministry of Economic Affairs, temporary Huicai exhibition staff W consumer cooperatives printed u, such as the scope of application for patent scope 1 or 9, 10 microscope image transmission system, where: It is determined by the ratio of the visual field size observed at a standard magnification to the visual field observed at a high magnification. 12, A microscope image processing method, the steps are characterized in that: the extraction step is to extract the luminance information of the still image containing the specimen image; and the setting is based on the luminance S signal related to the background position of the specimen image The maximum brightness level is set; the setting step is to set the minimum brightness level based on the brightness information related to the unwanted data position of the specimen image: the conversion step is based on the above maximum brightness level and the minimum brightness level. Transforming the above-mentioned luminance information: and the judging step is to judge the background position of the specimen image, the position of the unwanted data, and the position of the specimen image based on the converted luminance information; and extract the position of the specimen image. 13 " A microscope image processing method, the steps are characterized by: the extraction step, which is the color information of the specimen image; the identification step, which identifies the position where the color information is close to the maximum value as the background position of the specimen image ; The identification step is to identify the position where the above-mentioned color information is close to the minimum value as the unnecessary data position of the specimen image; and .I " "! --Line (guess first read the note on the back and fill in the lettuce page) A8B8C8D8 449662 6. The scope of patent application identification step is to identify the color information as the position above the threshold value as the position of the specimen image; and extract the position of the specimen image. 14 · —A method for processing microscope images, which is characterized by a step of extracting color information that is different from the same position of the specimen image; the step of obtaining the maximum position is that of obtaining color information from the same position of the specimen image The position where the difference becomes the largest; The step of setting a threshold value is based on the value of the difference between the above-mentioned color information being the maximum, and the predetermined threshold value is set; and the identification step 'is the difference of the above-mentioned color information according to the above-mentioned threshold. Limit value difference 'and the position above the threshold value or below the threshold value is identified as the position of the specimen image; and the position of the specimen image is extracted. 15. The microscope image transmission method according to item 14 of the scope of patent application, wherein the step of setting the threshold value set above further has: a detection step of detecting a maximum value of a difference in luminance between red and green; the detection step The maximum value of the difference between the blue and green brightness is detected: The threshold value step is to take the quotient of the maximum value of the above brightness difference at an arbitrary value, and use this value as the threshold value. 16 'If the microscope image processing method of the patent application No. 12 or 13, 14' has the following steps: Display step, after extracting the position of the specimen image, only the area where the specimen image exists The unit of the section is divided. The paper size is applicable to the China National Standard (CNSU4 specification (210 X 297 mm) 1111111111111 ^ — — — — — — 1 — ^ — — — — 1 — II * 1 v V ( Please read the notes on the back before filling out this page. ”Printed by the Consumers’ Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 52 A8B8C8D8 Employees ’Cooperative Cooperation of the Ministry of Economics and Wisdom Financial Staff Du Yin S. Applicants’ scope and will indicate this section The index is displayed superimposed on the still image containing the specimen image. 1 7 'A recording medium is a program used to record the position of the specimen image to be extracted and used by microscope image processors. The recording procedure is to include grapefruit brightness information' Extract the luminance information of the still image containing the specimen image; set the maximum luminance level so that it is in the background position of the specimen image The maximum luminance level is set based on the relevant luminance information; the minimum luminance level is set, and the minimum luminance level is pre-set based on the luminance information related to the unnecessary data position of the specimen image; The information is based on the above maximum luminance level and the minimum luminance level, and the above-mentioned luminance information is transformed. The judgment position is based on the transformed above-mentioned luminance information, and the background position of the specimen image is judged. The data position, the position of the specimen image, and the recording program are programs for recording the position where the specimen image is to be extracted. 18. A recording medium for recording the position where the specimen image is to be extracted and used for the microscope image processor The recording procedure consists of: extracting excellent information, extracting the color information of the specimen image; identifying the re-viewing position, identifying the position where the above-mentioned color information is close to the maximum value as the background position of the specimen image: ------ Ill — — — — — -----—II — — — — — — — < Please read the notes on the back before filling in this w) 44966 Ί A8B8CSD8 Employees 'Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs printed yellow 6. Application for profit-seeking area identification of unnecessary data locations is to identify the locations where the above-mentioned color information is close to the minimum value as unnecessary data locations; identifying specimen image locations' is The position where the above-mentioned color information becomes above the predetermined threshold value is identified as the position of the specimen image; and the recording program is a program for recording the position where the specimen image is to be extracted. A recording medium for recording a program for extracting the position of a specimen image for use by a microscope image processor '. The recording procedure includes: extracting excellent information, extracting color information that differs from the same position of the specimen image; The maximum position is the position where the difference in color information from the same position of the specimen image becomes the largest; the setting threshold value is based on the value of the difference between the above color information being the maximum and the predetermined threshold is set Value; identifying the position of the specimen image 'the difference between the above-mentioned color information is distinguished by the above-mentioned threshold value' and it will be a position above or below the threshold value to be identified as the position of the specimen image; and a recording program, It is a program for recording the location of the specimen image to be extracted. 20. If the recording medium (31) 'for microscope image processing is used in the scope of patent application No. 19, among which, the procedure for setting the threshold value set above also includes: The detection step 'detects the maximum value of the brightness difference between red and green; the detection step' detects the maximum value of the brightness difference between blue and green; and the setting step ' The maximum value of the brightness difference is applicable to any number of paper sizes in accordance with the Chinese National Standard (CNS) A4 specification (210x297 meals) -I l · · — — hlllllllt ^^ IIII 1 II — — — — — — — — — II «I I- 'f Please read the note f on the back before filling out this page} 54 A8B8C8D8 6. The value of the patent application range shall be the quotient, and this value shall be used as the threshold. 21. The recording medium 31 for microscope image processing according to item 17, 18, or 19 of the scope of patent application, wherein: after extracting the position of the specimen image, only the area in which the specimen image exists exists in a predetermined section Single-position segmentation, and the indicator indicating the segment is superimposed on the still image containing the specimen image and displayed as 0 -------------- installation, please read the ^ on the back first Completion of this book I) Order --------- Online-Printed by the Ministry of Economic Affairs 'Clear Wisdom Finance Bureau Employee Consumption Cooperatives' This paper size is applicable to China® Standard (CNS) A4 (21CU 297 mm) 〉