TW449662B - Microscope image transmitting system, method of processing microscope images, and recording medium for use in processing microscope images - Google Patents
Microscope image transmitting system, method of processing microscope images, and recording medium for use in processing microscope images Download PDFInfo
- Publication number
- TW449662B TW449662B TW089101991A TW89101991A TW449662B TW 449662 B TW449662 B TW 449662B TW 089101991 A TW089101991 A TW 089101991A TW 89101991 A TW89101991 A TW 89101991A TW 449662 B TW449662 B TW 449662B
- Authority
- TW
- Taiwan
- Prior art keywords
- image
- specimen
- specimen image
- information
- microscope
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
Landscapes
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Engineering & Computer Science (AREA)
- Image Processing (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
449662449662
五、發明說明(1 ) 經濟部智慧財產局員工消費合作社印製 本發明,係有關於-種例如,將在處理標本有關之顯 微鏡影像’欲於遠距作觀察m统,特別是,有關於 在上述標本之中,將欲擴大之領域以所定之區段(單元)作 指定,而對於㈣段可作更擴大_微㈣像傳送系 統,顯微鏡影像處理方法及使用於顯微鏡影像處理之記錄 媒體。 ' 習知’以視頻攝影機等予以攝影配置在顯微鏡之載物 台上的標本,再以個人電腦之影像捕捉埠口取進’並將該 影像作數位化,而經由例如,ISDN等之公用線路轉送至 配置在遠距處理之個人電腦,而在該個人電腦之監控器顯 示影像之顯微鏡影像傳送系統方面,已開發種種之技術。 有關該顯微鏡影像傳送系統,例如,被利用於病理醫師之 病理診斷等,而隨著由遠距之病理醫師的病理診斷之顯微 鏡操作或倍率變更、載物台移動亦成為可行。 例如,在特開平9-120031號公報,乃揭示將送訊端末 個人電腦(依賴側)與收訊端末個人電腦(觀察侧)經由isdn 線路連接’以進行計算該所指定之攝影位置之顯微鏡載物 台的絕對座標’且以該計算結果為根基進行載物台位置控 制之後’取入所欲觀察之影像為其特徵之技術。 通常,利用顯微鏡之病理觀察,依首先,病理醫師以 肉目艮觀察顯微鏡用標本整體像,而訂定概要之觀察方針, 接著以低倍率進行顯微鏡觀察,且進行初期診斷或以更高 倍率進行詳細觀察,惟,在上述系統,乃將如是之一連串 的診斷步驟於遠距(觀察侧)亦可實現》 本纸張仏適帛中國圏家標準(CNS)A4規格⑵Q χ 297公餐) 4 ----— — — — — — — — — .ίΛ {請先閲讀贵面之注意事項再填寫本頁) 經濟部智慧財產局W工消費合作ittn乾 A7 ______B7____ 五、發明說明(2 ) 另方面’在特開平6_22228 1號公報,乃揭示以低倍率 進行顯微鏡觀察之時,將標本像以所定之矩形領域作網目 分割’而在該所分割之領域之中選擇所要之領域,即,以 自動對於該選擇領域取入影像’為其特徵之系統。 又’在特開平9-1383 55公報,乃揭示將標本像以所設 疋之所疋的臨限值予以2值(2進位)化,並在作網目分割之 領域之中’削除不要之區段,為其特徵之技術。 但是’在利用前述之特開平6-222281號公報所揭示之 技術,將標本像作網目分割且對於該所分割之各部份,是 否要取入影像乃能確認。但是,其反面’對於由作業者欲 予網目分割之領域的起點及終點之指定的作業,以及網目 分割之後隨著有削除不要之部份的作業,因此,有操作煩 雜之課題發生。特別是,在手術上之診斷上,乃以不浪費 時間為最佳’因此,乃有其改善的要求。 而且,在由上述特開平9-138355號公報所揭示之技術 ,乃僅予指定網目取入位置之起點、終點,而依臨限值設 定就可自動削除不要之處所。但是,區段指定要依照固定 座標(x,y),因此,乃有無法作最適宜之區段指定之課題 〇 又,辨識灰麈等不要之資料,而有不能作正確之網目 分割指定之可能性存在°即,宏覲像之場合,由於可取入 之範圍較大之故,會取入顯微鏡用標本之端像,甚至亦會 取入壓著顯微鏡用標本之載物台夹子與顯微鏡用標本間之 空間=又‘從宏觀像欲予作低倍率之網目分割指定之場人 297公釐 -------------裝 i — (請先Μ讀-f面tii意事項*4填寫本頁) 訂: -線 A7 B7 五、發明說明(3 ) ,發生不要之影像部份的可能性較高,而將該等不要之部 份作為標本像辨識’而由於在網目分割之結果會產生誤差 ’乃要求其解決對策。 ^ 本發明因鑑於上述課題所進行,其目的係欲提供在顯 微鏡影像之遠距觀察上,有關在初期觀察影像上之觀察領 域的網目分割指定,依簡單之操作並有效率且能正確進行 之顯微鏡影像傳送系統、顯微鏡影像處理方法,及使用於 顯微鏡影像處理之記錄媒體。 為達成上述目的,於本發明之第〗態樣,係將在標本 像之中,欲以高倍率作觀察之領域以所定之區段單位獲得 上述標本像之攝影裝置,將包含由上述攝影裝置所攝影之 標本像之靜止影像作記錄之記錄裝置,顯示予以記錄在上 述記錄裝置之靜止影像的顯示裝置,以及抽出上述標本像 之位置且以其為根基,而僅將該標本像所存在之領域以所 定之區段單位作分割,並將表示該區段之指標重疊在上述 靜止影像,而具有予以顯示在上述顯示裝置之機能的控制 裝置。 於第2態樣’係將在標本像之中欲以高倍率作觀察之 領域,以所定之區段單位置作指定之顯微鏡影像傳送系統 ,乃具備攝影標本以獲得上述標本像之攝影裝置,記錄包 含由上述攝影裝置所攝影之標本像的靜止影像之記錄裝置 ,至少亦欲予顯示記錄在上述記錄裝置之靜止影像的顯示 裝置,於無上述標本之狀態下取入靜止影像,小於有上述 標本之狀態下取入靜止影像,並以兩者之輝度資訊之差值 本纸張&度適用中S1國家標準(CNS)A4規格(21CU 297公釐) (锖先Μ讀嘴面之注意事項再填寫本頁) 訂· -線 經濟部智慧时產局員工消費合作社印製 6 經濟部智慧財產局ΜΓ工消費合作社印Μ A7 -----—B7____ 五、發明說明(4 ) 為根基而予檢出光量不足部份之檢出裝置,以及將由上述 檢出裝置所檢出之光量不足部份予以除外之後,再由上述 靜止影像予以檢出標本像之位置,而僅將該標本像所存在 之領域以所定之區段單位置作分割,並將對該區段有關之 私‘重疊在上述靜止影像,而予控制在上述顯示裝置所作 之顯示的控制裝置。 於第3態樣,係至少亦在其任一方具備控制裝置之覲 察側與依賴側,乃予連接為相互可自由通信,而將在標本 像之中以高倍率欲予觀察之領域,以所定之區段單位作分 割之顯微鏡影像傳送系统,而i述依賴侧乃具有,攝影標 本以獲得上述標本像之攝影裝置’顯示包含由上述攝影裝 置所攝影之標本像的靜止影像之第丨顯示裝置,以及與上 述觀察側可進行資訊之送收訊的第1送收訊裝置且上述 觀察側乃具有欲予顯示從上述依賴側所傳送之靜止影像的 第2顯不裝置,以及與上述依賴側可進行資訊之送收訊的 第2送收訊裝置,而上述控制裝置,係具有顯示利用該控 制裝置之方的顯示裝置之靜止影像予以抽出標本像之位 置,且以其為根基而僅將該標本像所存在之領域以所定之 區段單位作分割,而將表示該區段之指標重疊在靜止影像 作顯示之機能,以及使未予利用上述控制裝置之一方的顯 不裝置之靜止影像,亦可成為與顯示有利用上述控制裝置 之一方的顯示裝置之靜止影像為同樣之影像,而從利用上 述控制裝置之-方對於未利用之一方,至少亦可將表示上 述區&之拓標本像的位置資訊予以送訊之機能,並且,於 雜 格 gI^T297 妓)--- . ------ I I (請先閱讀-f面之注意事項再填寫本頁} 訂 線· 449662 A7 B7 五、發明說明( 上述依賴側與觀察側共有同一資訊,為其特徵。 請 先 閱 讀 背 面 之 注 意 事 項 再 填 寫 本 買 於第4態樣’顯微鏡影像處理方法,係具有抽出包含 標本像之靜止影像的輝度資訊之步驟,依據對上述標本像 之背景位置有關之輝度資訊予以設定最大輝度準位之步驟 *依據於上述標本像之不要的資料位置有關之輝度資訊予 以設定最小輝度準位之步驟’以上述最大輝度準位及最小 輝度準位為根基,將上述輝度資訊作變換之步驟,以及對 該所變換之上述輝度資訊為根基而予判斷標本像之背景位 置’不要之資料位置、標本像之位置之步驟,並由此而抽 出標本像之位置。 於第5態樣,顯微鏡影像處理方法,係具有抽出標本 像之色資訊的步驟,將上述色資訊接近於最大值之位置與 標本像之背景位置作辨識之步驟,將上述色資訊接近於最 小值之位置,與標本像之不要的資料位置作辨識之步驟, 以及將上述色資訊成為所定之臨限值以上之位置,與標本 像之位置作辨識之步驟,而予抽出標本像之位置。 經濟部智慧財產局員工消費合作社印製 於第6態樣,顯微鏡影像處理方法,係具有於標本像 之同位置抽出相異之色資訊的步驟,求出在上述標本像之 同位置的色資訊之差成為最大之位置的步驟,以上述色資 訊之差成為最大之值為根基而予設定臨限值之步騾,以及 以上述臨限值予以區別上述色資訊,而將成為臨限值以上 或臨限值以下之位置,與上述標本像之位置作辨識之步驟 ,而予抽出標本像之位置。 於第7態樣,使用於顯微鏡影像處理之記錄媒體,係 本紙張尺度適用中國國家標準(CNS)A4規格(210 * 297公釐) 麵濟邹智慧財產局員工消費合作.吐印智 A7 B7 五、發明說明(6 ) 予以柚出包含標本像之靜止影像的輝度資訊之後,以對於 上述標本像之背景位置有關之輝度資訊為根基,而予以設 定最大輝度準位,同時,以對於上述標本像之不要的資料 位置有關之輝度資訊作為根基,而予以設定最小輝度準位 ,再依據上述最大輝度準位及最小輝度準位,而將上述輝 度資訊作變換,並以該所變換之上述輝度資訊為依據,而 予以判斷標本像之背景位置,不要之資料位置及標本像之 位置’而予記錄抽出標本像之位置的程式。 於第8態樣,使用於顯微鏡影像處理之記錄媒體,係 括出標本像之色資訊之後’將上述色資訊接近於最大值之 位置與標本像之背景位置辨識,同時,將上述色資訊接近 為最小值之位置與標本像之不要的資料位置辨識,並將上 述色資訊成為所定之臨限值以上之位置,與標本像之位置 辨識*而予記錄抽出標本像之位置的程式。 於第9態樣,使用於顯微鏡影像處理之記錄媒體,係 於標本像之同位置予以柚出相異之色資訊之後,求出在上 述標本像之同位置的色資訊之差成為最大之位置,且以上 述色資訊之差成為最大之值為依據而予設定所定之臨限值 ,再以上述臨限值予以區别上述色資訊之差,並將成為臨 限值以上或臨限值以下之位置與標本像之位置辨識,而予 以記錄柚出標本像之位置的程式。 本發明之實施態樣 以下,參照圖式說明本發明之實施態樣。 第1圖係本發明之顯微鏡影像傳送系統之概略圖。如 一 I __ 氏張i t这用气3S家墚茗(CN-S)A4見格(210 X 297公釐> 〇 I-----I------裝-------^訂--------線 {請先閱讀背面之注意事項再填寫本頁) 44966 2 A7 -—-----B7___ 五、發明說明(7 ) 在第1圖所7F,該顯微鏡影像傳送系統,係使觀察侧之個 人電腦1與依賴側之個人電腦5,介著未圖示之線路連接裝 置及公用線路(例如,ISDN)3予以連接為可自由通行。並 且,各個電腦1,5乃具備為顯示觀察像之用的顯示機能之 監控器2,4。 依賴側個人電腦5 ,又連接於顯微鏡該顯微鏡7乃 具備電動載物台9與電動旋轉器8及視頻攝影機6β有關之 顯微鏡7除該等之外,當然亦可具備未圊示之自動調焦裝 置(單元)、調光機構、電動光圈機構等e又於該例,乃採 用具備電動機能之顯微鏡,惟’並不限定於此,只要至少 亦具備電動載物台之顯微鏡,就能適用本發明。又’於本 實施態樣,公用線路乃使用ISDN,惟,並非限定於此, 例如,衛星線路等,而可依賴傳送資訊者就能適用。 經濟部智慧財產局員工消費合作社印製 上述個人電腦1,5,乃具有視頻捕捉器(未圖示)機能 ’且再具有接受上述視頻攝影機6之影像輸出的端子為首 之各種端子。又,上述個人電腦1,5,雖具有為記憶從顯 微鏡7等所輸出之影像資訊等之用的外部記憶部31,惟’ 使外部記憶部3 1在別體上作為周邊機器而予附屬Μ〇等之 記錄裝置,當然亦可以。 而於上述構成之外亦可在線路連接裝置上予以連接無 線話機。依該無線話機就不必要手持話筒,乃能予實現進 行個人電腦操作等且予會話。並且,於上述例,乃僅將顯 微鏡7連接在個人電腦5之構成,惟,亦可介著視頻選擇器 等而再將宏覲攝影裝置連接於個人電腦5 9 10 本纸張尺度適用中國國家標準(ΐ_Α4規格(il〇 X 297公爱·), A7 B7 經濟部智慧时產局員工消費合作社印製 五、發明說明( 第2圊係表示將第1圊之搆成再予具備化的機能方塊囷 。如在第2圖所示,該系統係由顯微鏡7,以及對於該顯微 鏡7輸出各種之控制信號或予收訊從裝設在顯微鏡7之攝影 部21的視頻信號,而進行將後述之處理的個人電腦5,所 構成。 上述顯微鏡7,係由攝影部21、倍率切換部22、載物 台移動部23、自動調焦部24、自動調整光圈部25所成。但 是’上述自動調焦部24與自動調整光圏部25可省略。 另方面,上述個人電腦5’乃由顯微鏡控制部12與内 部處理部13及使用者介面部14所成。上述顯微鏡控制部 ’係以畫面記憶部26、倍率控制部27、載物台控制部28 ' 焦點控制部29,及可變光圏控制部3〇所成。上述内部處理 部13,係以外部記憶部31,標本領域辨識部32、區段分割 部33、及倍率資訊與區段大小之相關關係資訊記憶部34所 成。上述使用者介面部14乃由顯示部35、倍率指定部36、 及區段追加、削除、移動指定部37所成。 上述外部記憶部31,係欲記憶將後述之流程圖的電腦 程式之記錄媒體’例如’硬碟或軟碟、CD-ROM、MO等 均能適用。 又’於第2圖乃以依賴側之構成為中心作說明,惟, 觀察側之個人電腦1的控制系統,係除去顯微鏡控制部】2 以外’乃與上述個人電腦5之控制系統大約為同樣之構成 之故,就予省略其說明。 以下,參照第3圖之流程圏,詳細說明上述構成之本 本紙張尺度適用t园因家棵準(CNS)A4规格<210 X 297公« ) ------裝-----—訂---------線 (請先閲讀背面之注意事項再填寫本頁) 11 449662 A7 B7 五、發明說明(9 ) 發明的顯微銳影像傳送系統之操作順序》 首先,於依賴側,將觀察側之病理醫師等所欲檢查之 標本像取入作為宏觀像(步驟Si)。於此,上述宏觀像,係 由未圖示之宏觀攝影裝置所取入之標本像,或者,以顯微 鏡7之最低倍率甚至同等倍率所取入之標本像。 於顯微鏡7之攝影部21所攝影之宏觀像,係介著依賴 側之個人電腦5内的未圖示之視頻捕捉埠口,而於畫面記 憶部26作為依序資料予以傳送,並予顯示在顯示部35(監 控器4)之畫面上。上述宏觀像之取入乃同步於任意之開關 操作而予開始。即,宏觀像之取入,係以依賴側之顯示器 35(監控器4上之應用軟體為根基,而將所顯示之操作SW 鈕以滑鼠予以抓取,或者,以未圖示之外部操作盤開關予 以選擇,而予開始。 接著,如上述予以取入宏觀像,即,依賴側之個人電 腦5 ’乃對觀察側之個人電腦1進行線路連接要求(步驟S2) 。該線路連接要求,乃介著未圖示之線路連接裝置、ISDN 等之公用線路3,而對觀察側之個人電腦1而將數位資訊予 以傳送而予進行。於該線路連接要求,乃予送訊線路連接 要求指令、初期資料、宏觀像資訊等。 覲察側之個人電腦1收訊上述線路連接要求指令、初 期資料、宏觀像資訊等(步驟S3),即,顯微鏡7之操作權 就變更於觀察側。而有關顯微鏡7之操作權之變更,於線 路連接確立之時間(時刻)以自動性進行亦可以。又,於顯 示部35之監控器2的畫面上,而依據應用軟體所顯示之操 本纸張尺度適用申國囷家楳準(CNS>A4规格(210x297公釐) it*先閱讀嘴面之注寺?i項再填寫本頁> 經濟部智慧財產局員工消費合作社印製V. Description of the invention (1) The invention is printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economics, which is related to-for example, the microscope images related to the processing of specimens, 'for observation at a distance, especially, there is about Among the above specimens, the area to be expanded is designated by a predetermined section (unit), and the segment can be further expanded_micro image transmission system, microscope image processing method, and recording medium used for microscope image processing . 'Xinzhi' uses a video camera to take a sample placed on the stage of a microscope, and then takes it through the image capture port of a personal computer 'and digitizes the image, for example, through a public line such as ISDN Various technologies have been developed for transferring to a personal computer configured for long-distance processing, and a microscope image transmission system in which the monitor of the personal computer displays images. The microscope image transmission system is used, for example, in pathological diagnosis of a pathologist, and as the microscope operation or magnification is changed by a remote pathologist, the stage movement becomes feasible. For example, in Japanese Patent Application Laid-Open No. 9-120031, it is disclosed that a transmitting terminal personal computer (dependent side) and a receiving terminal personal computer (observation side) are connected via an isdn line to calculate the microscope position of the designated photography position A technology that takes the absolute coordinates of the object and takes the calculated position as the basis to control the position of the object, and takes the image to be observed as its feature. Generally, pathological observation using a microscope is based on first observing the overall image of a microscope specimen by a pathologist and setting a summary observation policy, then performing microscopic observation at a low magnification, and performing an initial diagnosis or a higher magnification. Detailed observation, however, in the above system, such a series of diagnostic steps can also be achieved at a long distance (observation side). This paper is suitable for Chinese Standards (CNS) A4 specification (Q χ 297 meals) 4 ----— — — — — — — — — .ίΛ {Please read the precautions for your noodles before filling out this page) Intellectual Property Bureau of the Ministry of Economic Affairs, W Industrial Consumption Cooperation, ittn A7 ______B7____ V. Description of Invention (2) Others As for the aspect, in Japanese Unexamined Patent Publication No. 6_22228 1, it is revealed that when a microscope observation is performed at a low magnification, a specimen image is divided into meshes in a predetermined rectangular area, and a desired area is selected from the divided areas, that is, to A system that automatically captures images for this selected area as its features. Also, in Japanese Patent Application Laid-Open No. 9-1383 55, it is revealed that the specimen image is binarized (binary) to the threshold value set and set, and the unnecessary area is removed in the field of mesh division. Paragraph is the technology that characterizes it. However, using the technique disclosed in the aforementioned Japanese Unexamined Patent Publication No. 6-222281, the specimen image is divided into meshes, and whether or not the divided portions need to be taken into the image can be confirmed. However, on the other hand, the operation of designating the start and end points of the field to be divided by the operator and the operation of removing unnecessary parts after the division of the mesh are accompanied by a problem of complicated operation. In particular, in terms of surgical diagnosis, it is best not to waste time '. Therefore, there is a demand for improvement. In addition, in the technique disclosed in the above-mentioned Japanese Patent Application Laid-Open No. 9-138355, only the start point and end point of the mesh taking position are designated, and the unnecessary places can be automatically deleted according to the setting of the limit value. However, the section designation must follow the fixed coordinates (x, y). Therefore, there is a problem that the most suitable section designation cannot be made. Also, it is necessary to identify unnecessary data such as gray dust, and it is impossible to specify the correct mesh division designation. Possibility exists. That is, in the case of Acer images, because of the large range, the end images of microscope specimens may be taken, and even the stage clamps for microscope specimens and microscopes may be taken. Space between specimens = '297 mm from the macro image intended to be divided into meshes with a low magnification ------------- install i — (please read -f 面 tii first Matters needing attention * 4Fill this page) Order:-Line A7 B7 V. Invention description (3), there is a high possibility that unwanted image parts will occur, and these unwanted parts are identified as specimen images' because The result of the mesh segmentation will cause errors', which is required to solve the problem. ^ The present invention has been made in view of the above-mentioned problems, and its purpose is to provide the designation of the mesh division of the observation field on the initial observation image in the long-distance observation of the microscope image, with simple operation, efficient and accurate. Microscope image transmission system, microscope image processing method, and recording medium used for microscope image processing. In order to achieve the above object, in the first aspect of the present invention, a photographing device that obtains the specimen image in a predetermined section unit in a specimen image in an area to be observed at a high magnification will include the photographing device described above. A recording device for recording a still image of a photographed specimen image, a display device for displaying the still image recorded on the recording device, and a position where the specimen image is extracted and used as a basis, and only the specimen image exists. The field is divided by a predetermined section unit, and an indicator indicating the section is superimposed on the still image, and has a control device for displaying the function on the display device. In the second aspect, the microscope image transmission system will be used in the specimen image to be observed at a high magnification and designated by a single position in the specified section. It is a photographing device equipped with a photographic specimen to obtain the aforementioned specimen image. A recording device that records a still image containing a specimen image taken by the above-mentioned photographing device, at least also a display device that wants to display the still image recorded on the recording device, and takes the still image without the above-mentioned specimen, which is smaller than the above Take a still image in the state of the specimen, and use the difference between the luminance information of the two. The paper & degree is applicable to the S1 National Standard (CNS) A4 specification (21CU 297 mm). Please fill in this page for the matters) Order-Printed by the Consumers' Cooperative of the Wisdom Time and Industry Bureau of the Ministry of Economic Affairs 6 Printed by the Intellectual Property Bureau of the Ministry of Economy ΜΓ Industrial and Consumer Cooperative Cooperatives A7 ------B7____ 5. The invention description (4) is the foundation The detection device that detects the insufficient light amount and excludes the insufficient light amount detected by the detection device, and then detects the specimen image from the still image. Only the area in which the specimen image exists is divided by the specified single position of the section, and the private information related to the section is superimposed on the still image, and the display control on the display device is controlled Device. In the third aspect, the observation side and the dependent side, which have control devices at least on either side, are connected so as to be able to communicate freely with each other, and will be observed in the specimen image at a high magnification, in order to The microscope image transmission system is divided by a predetermined segment unit, and the dependent side is provided with a photographing device for photographing a specimen to obtain the specimen image, and displaying a still image including a still image of the specimen image photographed by the photographing device. Device, and a first transmitting and receiving device capable of transmitting and receiving information to and from the observation side, and the observation side is a second display device for displaying a still image transmitted from the relying side, and is dependent on the above A second transmitting and receiving device capable of transmitting and receiving information is provided on the side, and the above-mentioned control device has a position for extracting a specimen image by displaying a still image of the display device using the control device, and using it as the basis, only The area in which the specimen image exists is divided by a predetermined section unit, and an indicator indicating the section is superimposed on a still image for display, and The still image using the display device of one of the above control devices can also be the same image as the still image displayed by the display device of one of the above control devices. At least one party can send the information about the location information of the specimens of the above-mentioned area & at the same time, and in the g1 ^ T297 prostitute) ---. ------ II (Please read- Note on the f side, please fill out this page again} Ordering line · 449662 A7 B7 V. Description of the invention (The above-mentioned relying side and observation side share the same information, which is its feature. Please read the notes on the back before filling out this buy in the fourth state Sample 'microscope image processing method has the steps of extracting the luminance information of the still image containing the specimen image, and setting the maximum luminance level based on the luminance information related to the background position of the specimen image. The step of setting the minimum luminance level for the luminance information related to the data position of 'is based on the above maximum luminance level and minimum luminance level, The step of transforming the above-mentioned luminance information, and determining the background position of the specimen image 'unwanted data position, the position of the specimen image based on the transformed luminance information as a basis, and extracting the position of the specimen image from this In the fifth aspect, the microscope image processing method has a step of extracting color information of the specimen image, identifying the position where the color information is close to the maximum value and the background position of the specimen image, and bringing the color information close to The step of identifying the position of the minimum value from the unnecessary data position of the specimen image, and the step of identifying the position of the specimen image by the above-mentioned color information to a position above a predetermined threshold, and extracting the position of the specimen image The Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs printed in the sixth aspect. The microscope image processing method has the steps of extracting different color information at the same position of the specimen image, and finding the color at the same position of the specimen image The step of the position where the information difference becomes the largest, and the threshold value is set based on the value of the difference between the color information as the maximum. Steps, and distinguishing the above color information with the above threshold value, will be a step above the threshold value or below the threshold value to identify the position of the specimen image and extract the position of the specimen image. In the seventh aspect, the recording medium used for microscope image processing is the paper size applicable to the Chinese National Standard (CNS) A4 specification (210 * 297 mm). The cooperation of the employees of the Nian Zou Intellectual Property Bureau. Tuyinzhi A7 B7 V. Description of the invention (6) After giving the brightness information of the still image containing the specimen image, the maximum brightness level is set based on the brightness information related to the background position of the specimen image, and at the same time, the above-mentioned specimen is set. The brightness information related to unnecessary data positions is used as the basis, and the minimum brightness level is set, and then the brightness information is transformed according to the above maximum brightness level and minimum brightness level, and the transformed brightness is used as the basis. Based on the information, it is necessary to determine the background position of the specimen image, the position of the unnecessary data and the position of the specimen image, and record the program position of the extracted specimen image. In the eighth aspect, the recording medium used for microscope image processing includes the color information of the specimen image, and 'identifies the position where the above-mentioned color information is close to the maximum value and the background position of the specimen image, and at the same time, the above-mentioned color information is close to It is a program that records the position of the extracted sample image by identifying the position of the minimum value and the position of unnecessary data of the specimen image, and using the color information as a position above a predetermined threshold value and the position identification of the specimen image *. In the ninth aspect, the recording medium used for microscope image processing is based on the difference in color information at the same position of the specimen image, and then the position where the difference between the color information at the same position of the specimen image becomes the largest is determined. , And set a threshold value based on the difference between the above color information becoming the maximum value, and then use the above threshold value to distinguish the difference between the above color information, and it will be above the threshold value or below the threshold value The program recognizes the position of the specimen image and records the position of the specimen image. Embodiments of the present invention Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a schematic diagram of a microscope image transmission system of the present invention. For example, I __ 's Zhang it this gas 3S furniture (CN-S) A4 see the grid (210 X 297 mm)> 〇I ----- I ------ installation ------ -^ Order -------- line {Please read the notes on the back before filling out this page) 44966 2 A7 --------- B7___ V. Description of the invention (7) In Figure 7F of Figure 1, This microscope image transmission system is such that the personal computer 1 on the observation side and the personal computer 5 on the dependent side are connected to each other via a line connection device (not shown) and a public line (for example, ISDN) 3 that are freely accessible. In addition, each of the computers 1, 5 is provided with monitors 2, 4 for displaying functions for displaying an observation image. The personal computer 5 on the dependent side is connected to the microscope. The microscope 7 is provided with a motorized stage 9 and a microscope 7 related to a motorized rotator 8 and a video camera 6β. Of course, it can also have an unfocused autofocus. The device (unit), dimming mechanism, electric diaphragm mechanism, etc. e are again used in this example, but a microscope equipped with motor power is used, but 'is not limited to this, as long as it also has at least a microscope with a motorized stage, the present invention is applicable invention. Also, in this embodiment, the public line uses ISDN, but it is not limited to this, for example, a satellite line, and it can be applied by a person who can rely on transmitting information. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, the above personal computers 1,5 have various functions including a video capture (not shown) function and a terminal that accepts the video output from the video camera 6. In addition, although the personal computers 1, 5 have an external memory section 31 for storing image information and the like output from the microscope 7, etc., the external memory section 31 is attached to a peripheral device as a peripheral device. 〇 and other recording devices, of course. In addition to the above configuration, a wireless telephone can be connected to the line connection device. According to this wireless telephone, there is no need to hold a microphone, and it is possible to carry out personal computer operation and the like and to talk. In the above example, only the microscope 7 is connected to the personal computer 5. However, it is also possible to connect the Acer photography device to the personal computer 5 9 10 through a video selector and the like. Standard (ΐ_Α4 specifications (il0X 297 public love ·), A7 B7 Printed by the Consumers' Cooperative of the Wisdom and Time Bureau of the Ministry of Economic Affairs. 5. Description of the invention (The second one indicates the function of rebuilding the first one) Box 囷. As shown in FIG. 2, this system uses a microscope 7 and outputs various control signals to the microscope 7 or receives and receives video signals from the photographing section 21 installed in the microscope 7, and will be described later. The processing personal computer 5 is composed of the above-mentioned microscope 7. The microscope 7 is composed of a photographing section 21, a magnification switching section 22, a stage moving section 23, an automatic focusing section 24, and an automatic aperture adjusting section 25. However, the above-mentioned automatic The focusing unit 24 and the auto-tuning unit 25 can be omitted. On the other hand, the personal computer 5 'is formed by the microscope control unit 12, the internal processing unit 13, and the user interface portion 14. The microscope control unit is based on a screen. Memory section 26 , Magnification control unit 27, stage control unit 28 ', focus control unit 29, and variable light control unit 30. The internal processing unit 13 is an external memory unit 31, a specimen area identification unit 32, and a zone. The segmentation section 33 and the information storage section 34 for the relationship between the magnification information and the segment size. The user interface part 14 is composed of the display section 35, the magnification designation section 36, and the segment addition, deletion, and movement designation section 37. The above-mentioned external memory unit 31 is a recording medium of a computer program that is intended to memorize a flowchart described later, such as' hard disk or floppy disk, CD-ROM, MO, etc., and it is also applicable to the second figure. The configuration of the reliance side is mainly described, but the control system of the personal computer 1 on the observation side is excluding the microscope control unit] 2 because it is approximately the same as the control system of the personal computer 5 described above, and is omitted. In the following, referring to the flow chart in Fig. 3, the detailed paper size of the above-mentioned paper will be explained in detail. Applicable to CNS A4 specifications < 210 X 297 male «) ------ installation- ----- Order --------- Line (Please read the note on the back first Please fill in this page again for details) 11 449662 A7 B7 V. Description of the invention (9) Operation sequence of the invented micro sharp image transmission system "First, on the relying side, take the specimen image of the observing side, such as a pathologist, to check As a macro image (step Si), the above macro image is a specimen image taken from a macro photography device (not shown), or a specimen image taken at the lowest magnification of the microscope 7 or an equivalent magnification. The macro image taken by the photography section 21 of the microscope 7 is transmitted through the screen memory section 26 as sequential data through a video capture port (not shown) in the personal computer 5 on the dependent side, and displayed on the display. Screen of the monitor 35 (monitor 4). The above macro image acquisition is started in synchronization with any switching operation. That is, the macro image is taken based on the display 35 on the dependent side (application software on the monitor 4), and the displayed operation SW button is captured by a mouse, or by an external operation (not shown) The disc switch is selected and started. Next, a macro image is taken as described above, that is, the personal computer 5 'on the dependent side makes a line connection request to the personal computer 1 on the observation side (step S2). It transmits the digital information to the personal computer 1 on the observation side through a line connection device (not shown), a public line 3 such as ISDN, etc. The connection request for this line is a transmission line connection request instruction, Initial data, macro image information, etc. The personal computer 1 on the inspection side receives the above-mentioned line connection request instruction, initial data, macro image information, etc. (step S3), that is, the operation right of the microscope 7 is changed on the observation side. The change of the operation right of the microscope 7 may be performed automatically at the time (time) when the line connection is established. In addition, on the screen of the monitor 2 of the display section 35, depending on the application The size of the paper shown in this application is applicable to the standard of the State Council of China (CNS > A4 (210x297 mm)) * Read the note temple on the mouth first? I then fill out this page > Employee Consumer Cooperatives, Intellectual Property Bureau, Ministry of Economic Affairs Print
^Γ ^ ^ I I I *-6J ί I - I n n n I I 12 A7 B7 五、發明說明(10 ) 作按鈕予以切換為使觀察側之操作者(病理醫師),以滑鼠 抓取之適合時間當然亦可以a 其次,觀察側就予指定顯微鏡7之對物倍率(步驟S4) 。該倍率指定,可將於觀察側之個人電腦1的顯示部3 5之 監控器2的畫面上’依據應用軟體所顯示之操作按紐’以 滑鼠予以抓取,或手操作於觀察側之個人電腦1的未圖示 之操作部的鍵盤上而事先所安排之所定鍵’就能進行。如 是,所指定之倍率資訊即適宜送訊至顯微鏡7之依賴側。 其次,覲察者(獻理醫師)就對欲予擴大之部份作網目 指定或點指定之選擇(步驟S5)。於此,上述「網目指定」 ,係將宏觀像分割為格子狀(以下稱為區段)而予指定擴大 位置者。另方面,「點指定」’係以任意之位置作為中心 而予指定1處所擴大位置者。以該「網目指定」或者「點 指定」之選擇為根基’而對在觀察側之顯示部35的監控器 (上)所顯示之標本像’進行線覆蓋,因此,觀察者(病理 醫師)能容易確認位置。 又,於本發明,係隨著上述銳察側之網目指定或者點 指定,而將該位置資訊對依賴側作隨時資料通訊,因此, 於依賴側之顯示部(35)(監控器4亦能與觀察側共有同樣之 畫面。 有上述步驟S5,於選擇網目指中 曰扣疋之%合,就進行網 目處理(步驟S6)而於選擇點指定之 火炙%合,訧進行點處理( 步驟S7)=該等之中,於網目處理 没主上可圖效率化,高精度 化方面乃為本發明之待徵之一。對 "於該網目處理,將使用 木铁張 <,至涑用由a國家樓準(CNSM4規格<210 X 297公 -------------裝- ----— —訂---------線 (請先閲讀背面之注意事項再填寫本頁) 13 A7 B7 經濟部智慧財產局員工消費合作杜印製 五、發明說明(11 ) 各種實施態樣而於後述。 以上述點處理或者網目處理為依據所指定之位置(領 域)方面’乃將以指定倍率之影像取入要求,對於依賴側 之個人電腦5進行(步驟S8)。又,上述所指定之位置資訊 ,乃予記錄在依賴側之個人電腦5與觀察側之個人電腦1的 内部之未圖示的記憶體等之記錄媒體(以下,稱為資訊記 憶裝置)。 接著’將從上述覲察側之個人電腦1的影像取入要求 ,於依賴側之個人電腦5收訊時,依賴側之個人電腦$就進 行顯微鏡7之操作’而取入對從觀察側之個人電腦1之要求 相對稱之影像(步驟S9)。 即,於上述顯微鏡操作’將以網目或者點所指定之位 置變換為載物台座標位置’且予驅動載物台驅動部23(電 動載物台9)而使標本移動,並因應指定倍率而將倍率切換 部22(電動旋轉器8)作變換。其後,以攝影部21取入標本 影像資訊。然後’將上述所取入之影像資訊取入於依賴側 之個人電腦5内的視頻捕捉埠口,並將影像變換為靜止畫 面。作成該靜止畫面之影像,將其原狀予以記錄在硬碟或 M0等之外部記憶裝置31亦可以,惟,於本發明,為圖影./ 像資訊轉送之方便,乃以JPEG形式等作影像壓縮之後而 作記憶。如是,記憶在外部記憶裝置31之影像資訊,就介 著未圖示之線路連接裝置、公用線路3,而從依賴側之個 人電腦5至觀察侧之個人電腦1予以作資訊轉送(步驟Si0) (請先閲讀嘴面之注意事項再填寫本頁) ϋ I t I n n IT I Ιϋ 11 n IV n It . 本紙張尺度適用中國國家標準(CNS)A4規格(210 X 297公釐) 14 A7 B7 經濟部智慧財產局員工消費合作ii,s· .¾ 五、發明說明(I2 ) 從上述依賴側個人電腦5所轉送之影像資訊,於觀察 側之個人電腦1收訊之時(步驟s丨]),觀察側個人電腦】乃 將所收訊之影像資訊予以顯示在觀察側顯示部35之監控器 2的晝面上。而如前述,影像資訊乃以JpEG方氏等作影像 壓縮之場合,即,於顯示之時就同時作所定之伸展處理。 如疋,所有之影像轉送終了之時,於觀察側就進行遠 距觀察而作診斷(步驟S12)。此時,將影像之連結與滑鼠 位置資訊等,就從有操作權之觀察側個人電腦丨’而通過 線路連接裝置及公用線路3轉送至依賴側個人電腦5。收訊 上述影像連結 > 訊或滑鼠位置資訊之依賴側個人電腦5, 就以該等之資訊為依據而將影像顯示於依賴側之顯示部 3 5(監控器4),且亦連結滑鼠位置等之資訊。而通常,由 於达距診斷乃由觀察側之個人電腦1予以進行,因此,操 作權乃在於觀察側,惟,亦可將該操作權任意予以切換於 依賴側之個人電腦5。 如上述,進行遠距觀察之後,就予進行為終了或否之 判斷(步驟Si4、S15)。於此,欲將物鏡擴大為高倍而繼續 作診察之場合’即,回復於上述步驟84而反覆上述一連 串之處理,而不欲予繼續作診察之場合,就予終止所有之 處理。又,若從觀察側對依賴側無終了要求之場合,就回 復至上述步驟S9而成為等待狀態。 於本發明,在上述順序之中,乃以步驟%之網目處 理之改善作為目的。即,將宏覲像之資料收訊後的網目分 割指定予以著眼在容易正確實現a以下,針對其有關方 、纸張义4過用中S國家俤承(CNS) 規格(210 X 297公餐 -------—-----裝--------訂·--------線 (請先M讀脅面之注意事項再填寫本頁) 15 經濟部智慧財產局員工消費合作社印^; 449662 A7 B7 五、發明說明(13 ) 之第1至7實施態樣作詳細說明。 《第1實施態樣》 首先,參照第4圖予說明對第1實施態樣有關之依顯微 鏡影像傳送系統作標本像位置之影像辨識的一連串之處理 〇 而於以下所欲說明之標本像的位置之影像辨識,於後 述之第2至第6實施態樣上述亦所採用之技術。 開始,於依賴側,以顯微鏡透過照明予以攝影病理標 本之場合,就可獲得如在第4A圖所示之影像。此場合, 背景部份51為白色而標本像52由於有染色,因此,帶有顏 色,且雜音部份53通常為接近於黑色《雜音部份53,例如 ’以宏觀像予以攝影之時容易發生,於攝影顯微鏡用榡本 及固定其之夾子之間的載物台部份之場合發生。又,其他 之主因為附著在光學系統之灰塵亦會被辨識為靜止影像之 V雜音部份53。於本實施態樣,係以標本之輝度資訊作為根 基而予進行標本像之位置的影像辨識,而所抽出之如是影 像的輝度資訊,就予格納在個人電腦5, 1内部之未圖示的 資訊記憶裝置上。在該資料記憶裝置上,例如,予以攝影 VGA(640X480)之影像的場合,即予格納以(640,480)之2^ Γ ^ ^ III * -6J ί I-I nnn II 12 A7 B7 V. Description of the invention (10) The button is switched to enable the operator (pathologist) on the observation side to grab the mouse with a suitable time. May be a second, the objective side magnification of the microscope 7 is designated on the observation side (step S4). This magnification designation can be grasped by a mouse on the screen of the monitor 2 of the display section 3 5 of the personal computer 1 on the observation side according to the operation button displayed by the application software, or manually operated on the observation side. The keypad of the operating unit (not shown) of the personal computer 1 can be assigned to a predetermined key. If so, the specified magnification information is suitable for sending to the dependent side of the microscope 7. Next, the inspector (dedicating physician) makes a selection of the network designation or the point designation for the part to be expanded (step S5). Here, the above-mentioned "mesh designation" refers to those who divide a macro image into a grid shape (hereinafter referred to as a section) and designate an enlarged position. On the other hand, "point designation" is a designation of an expanded location with one arbitrary position as the center. Based on the selection of the "mesh designation" or "point designation", the specimen image 'displayed by the monitor (top) of the display unit 35 on the observation side is line-covered, so that the observer (pathologist) can Easy to confirm location. Furthermore, in the present invention, the position information is communicated to the relying side at any time with the mesh designation or point designation of the sharp inspection side. Therefore, the display unit (35) (monitor 4 can also There is the same screen as the observation side. With the above step S5, in the selection of the mesh means, the percentage of the deduction is combined, and the mesh processing is performed (step S6). At the selected point, the flame percentage is combined, and the point processing is performed (step S7) = Among them, it is possible to improve the efficiency of the mesh processing, and the aspect of high precision is one of the features of the present invention. For the mesh processing, a wooden iron sheet will be used.涑 Used by a country building standard (CNSM4 specifications < 210 X 297 male ------------- install-------order --------- line (please (Please read the notes on the back before filling this page) 13 A7 B7 Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Cooperation Du V. Invention Description (11) Various implementation aspects will be described later. Based on the above point processing or net processing Regarding the specified position (area), it is a request to acquire the image at a specified magnification. 5 (step S8). The position information specified above is a recording medium (hereinafter, referred to as a recording medium, etc.) which is recorded in the personal computer 5 on the dependent side and the personal computer 1 on the observation side. It is an information storage device). Then "retrieve the image request from the personal computer 1 on the observation side, and when the personal computer 5 on the dependent side receives the data, the personal computer $ on the dependent side performs the operation of the microscope 7". Into the symmetrical image of the personal computer 1 from the observation side (step S9). That is, in the above-mentioned microscope operation, the position designated by the mesh or point is converted into the position of the stage coordinate, and the stage is driven. The driving unit 23 (motorized stage 9) moves the specimen, and the magnification switching unit 22 (electric rotator 8) is changed in accordance with the designated magnification. Thereafter, the specimen image information is taken by the photographing unit 21. Then, 'the The acquired image information is taken into the video capture port in the personal computer 5 on the dependent side, and the image is converted into a still picture. The image of the still picture is made and recorded in its original state on a hard disk or M0, etc. The external memory device 31 may also be used, but in the present invention, it is convenient for image and image transfer, and it is stored after compressing the image in JPEG format. If so, the image information stored in the external memory device 31, Information transmission is performed from the personal computer 5 on the relying side to the personal computer 1 on the observation side via the line connection device and public line 3 (step Si0) (please read the precautions on the mouth first and then fill out this (Page) ϋ I t I nn IT I Ιϋ 11 n IV n It. This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 14 A7 B7 Employees ’cooperation with the Intellectual Property Bureau of the Ministry of Economic Affairs ii, s · V. Description of the invention (I2) When the image information transmitted from the personal computer 5 on the dependent side is received by the personal computer 1 on the observation side (step s 丨), the personal computer on the observation side] The video information is displayed on the daytime surface of the monitor 2 of the observation-side display portion 35. As mentioned above, when the image information is compressed using JpEG Fang's, etc., that is, when the image is displayed, the predetermined stretch processing is performed at the same time. If all the images have been transferred, a remote observation is performed on the observation side for diagnosis (step S12). At this time, the connection of the image and the position information of the mouse are transferred from the observation-side personal computer with operation right to the personal computer 5 through the line connection device and the public line 3. Receiving the above image link > personal computer 5 on the relying side for information or mouse position information, based on such information, displays the image on the display part 3 5 (monitor 4) on the relying side, and also links the slide Mouse location and more. In general, since the distance diagnosis is performed by the personal computer 1 on the observation side, the operation right is on the observation side, but the operation right can be arbitrarily switched to the personal computer 5 on the dependent side. As described above, after the long-distance observation is performed, a judgment is made as to whether it is finished or not (steps Si4 and S15). Here, if the objective lens is to be enlarged to a high magnification and the diagnosis is to be continued, that is, to return to the above-mentioned step 84 and repeat the above-mentioned series of processing, and if no further examination is to be performed, all the processing is terminated. If there is no final request from the observation side to the relying side, the process returns to step S9 to wait. In the present invention, in the above sequence, the purpose is to improve the mesh processing of step%. That is, the designation of the mesh division after receiving the data of the Acer image should be aimed at achieving the following correctly and easily, and the relevant parties and papers will be used in the National Sector (CNS) specification (210 X 297 meals). -------------- Installation -------- Order · -------- line (please read the precautions of the threat surface before filling out this page) 15 Economy Printed by the Intellectual Property Cooperative of the Ministry of Intellectual Property, 449662 A7 B7 V. Detailed description of the first to seventh implementation aspects of the invention description (13). "First implementation aspect" First, referring to FIG. A series of processing related to the image recognition of the specimen image position according to the microscope image transmission system. The image recognition of the position of the specimen image to be described below is described in the second to sixth embodiments described below. The technology used. At the beginning, when the pathological specimen is photographed with the microscope through the illumination on the relying side, the image shown in Figure 4A can be obtained. In this case, the background portion 51 is white and the specimen image 52 is Dyed, therefore, with color, and the noise part 53 is usually close to black For example, 'prone to occur when taking photographs with a macro image. Occurs when the stage between the copy of the microscope for photography and the clip holding it. Also, other masters may be damaged by dust attached to the optical system. It is identified as the V noise part 53 of the still image. In this embodiment, the image recognition of the position of the specimen image is performed based on the luminance information of the specimen, and if the extracted luminance information is the image, it is given to Genna in On the information storage device (not shown) inside the personal computer 5, 1. On this data storage device, for example, when taking a picture of VGA (640X480), it is given 2 to (640, 480).
次元所排列之影像的輝度資訊。影像資訊,係分成r、G 、8之3種資訊,並以 R(640、480)、G(640、480)、b(640 、480)予以格納各畫格資訊。該等之中,使用為輝度資訊 者乃是以其形狀較易辨識之G(640、480)之資訊為宜。 將在第4A圖所示之標本像,如上述予以排列為2次元 本紙張尺度適用中國國家標準(CNS)A4規格(210x 297公爱) 16 (請先Μ讀背面之注意事項再填寫本頁)The luminance information of the image arranged by the dimension. The image information is divided into three types of information: r, G, and 8; and R (640, 480), G (640, 480), and b (640, 480) are used to provide information for each frame. Among these, it is appropriate to use the information of G (640, 480) whose shape is easier to recognize. The specimen images shown in Figure 4A are arranged as a two-dimensional paper as described above. The paper size applies the Chinese National Standard (CNS) A4 specification (210x 297 public love). 16 (Please read the precautions on the back before filling this page. )
A7 A7 經濟部智慧时產局員工消費合作社印敦 五、發明說明(w)A7 A7 Consumers' Cooperative, Indun, Employees, Smart Time and Industry Bureau, Ministry of Economic Affairs 5. Description of Invention (w)
而予格納在資訊記憶f置内之場合,背景部份51,Μ g、b均使畫格資訊接近於F⑽),而雜音部份53,R、G 、B均成為某臨限值以下(接近糾),且標本像如存在之 處理所,乃成為收納在所定範圍内之資料。 於此,在第4A圖所示之標本像上,予以抽出任意之γ 座標(Yi)之輝度資訊,即成為如在第扣圖所示。以該特性 為根基’而予設定標本像上限輝度準位55與標本像下限輝 度準位5 6。 該輝度準位之上限,以辨識背景部份51之輝度就能予 以設定。在具趙上乃依下述方法。即,操作者(觀察側或 依賴側)以;、骨鼠抓取背景部份51而作指巾,並將對應於滑 鼠指示箭頭之位置的畫像之輝度資訊,從上述資料記憶裝 置上予以讀出,並以其作為背景之輝度準位,且以比該值 稍低之值設定為標本像上限輝度準位35。或者,將標本像 欲予格納在資料記憶裝置上之時,予以檢出連續性反覆尺 、G、B均為接近於FF(255)之值的區段,而將該區段作為 为景予以自動辨識,且將該區段之畫格資訊予以平均化之 1料作為背景之輝度準位,並以此該值為稍低若干之值予 以设定作為標本像上限輝度準位55,當然亦可以。 至於標本像下限輝度準位56,亦與標本像上述限輝度 準位55同樣’操作者以滑鼠抓取雜音部份53而予指示,而 將對應於該滑鼠位置之影像的輝度資訊,由上述資料記憶 裝置予以柚出,且以其作為雜音準位,並以該值為稍高若 干之值設定為標本下限輝度準位56ls而上限、下限值均由 -------------裝--------訂-------•線 (諳先閲讀脅面之注意事項再填寫本X ) 家埤m (CNS)A·!規格297公釐) 449662 Δ7 B7 經濟部智慧財產局員工消費合作社印製 五、發明說明(15 ) 操作者可任意予以變更。 當上述設定終了之後’對於不在標本像上限輝度準位 55與標本像下限輝度準位56之範圍者,全部將輝度準位作 為FF(2 55)。作該資料變換之結果’就成為如在第圖所 示,僅有標本像之處所,其輝度準位乃成為〇。其次,將 所變換之資料為根基而重新予以顯示標本像,即如在第4D 圖所示’僅標本像之處所顯示黑色。變換後之禪度資訊就 以2次元之排列資料再輸入至資料記憶裝置,而僅有標本 像之畫格,其輝度準位成為0而其以外者就成為輝度準位 FF(255)之值。 於本發明之第1實施態樣,乃以上述之標本像的上限 、下限輝度資訊予以進行標本像之範圍檢出,同時,將參 照先前格納在資料記憶裝置之2次元的排列資料,而予執 行所定之網目指定。 以下’參照第5圊之流程圖及第6圖之表示例予以說明 第1實施態樣之網目資料作成之過程。 首先’如在第6A圖所示,依據在上述2次元所排列之 輝度資訊,自動檢出標本像所存在之最小座標(Xmin、 Ymin)與最大座標(Xmax,Ymax)(步雜S21)。 接著,由指定倍率算出指定區段之寬度、高度(步驟 S22)。該指定倍率,能將依據應用軟體所顯示之按鈕Sw 由滑鼠抓取予以確定,或對個人電腦之鍵盤等的機能(作 用)鍵予以分割配倍率而作指定。關於上述指定區段之莧 度、高度之演算,於此,就以將現在之物鏡的倍率作為基 (諳先閱讀嘴面之注意事項再填寫本頁) 本紙張尺度適用4*國國家標準(CNS)A4規格(210 * 297公釐)In the case where Yugner is in the information memory f, the background part 51, MG, and b make the frame information close to F⑽), and the noise part 53, R, G, and B all fall below a certain threshold ( (Close to correction), and the specimen image, if it exists, becomes the data stored in the predetermined range. Here, from the specimen image shown in FIG. 4A, the luminance information of arbitrary γ coordinate (Yi) is extracted, and it becomes as shown in the figure. Based on this characteristic, the specimen image upper limit luminance level 55 and the specimen image lower limit luminance level 56 are set. The upper limit of the luminance level can be set to identify the luminance of the background portion 51. On Gu Zhao, follow the method below. That is, the operator (observation side or dependent side) takes the background portion 51 as a finger towel with the bone mouse, and the brightness information of the image corresponding to the position of the mouse pointer arrow is obtained from the data storage device. Read out and use it as the background luminance level, and set a value slightly lower than this value as the specimen image upper limit luminance level 35. Alternatively, when the specimen image is intended for Gna on the data storage device, a segment with continuous repeat rule, G, B is close to the value of FF (255), and this segment is used as a scene Automatically recognize, and average the frame information of the segment as the background brightness level, and set this value to a slightly lower value as the upper limit brightness level of the specimen image 55, of course can. As for the lower limit brightness level 56 of the specimen image, it is the same as the above-mentioned limit brightness level 55 of the specimen. 'The operator grabs the murmur part 53 with a mouse and instructs it, and the brightness information of the image corresponding to the position of the mouse is indicated. The above data memory device is used to make the grapefruit, and it is used as the noise level, and the value is set to a slightly higher value as the specimen lower limit luminance level 56ls, and the upper and lower limits are both ------- ------ Equipment -------- Order ------- • Line (谙 Read the precautions of the flank first and then fill in this X) Home 埤 m (CNS) A ·! Specification 297 (Mm) 449662 Δ7 B7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs V. Invention Description (15) The operator can change it at will. After the above setting is completed, the brightness level is set to FF (2 55) for all those that are not within the range of the specimen image upper limit luminance level 55 and the specimen image lower limit luminance level 56. As a result of this data transformation, as shown in the figure, only the specimen image has a brightness level of 0. Secondly, the transformed data is used as the basis and the specimen image is displayed again, that is, as shown in Fig. 4D ', only the specimen image is displayed in black. The transformed Zen information is input to the data storage device in a two-dimensional array, and only the frame of the specimen image has a luminance level of 0 and the others become the value of the luminance level FF (255). . In the first embodiment of the present invention, the range of the specimen image is detected by using the above upper limit and lower limit luminance information of the specimen image, and at the same time, it will refer to the previous 2-dimensional array data of Genna in the data storage device, and Perform the specified mesh designation. The following process will be described with reference to the flowchart in FIG. 5 and the example of the display in FIG. 6 in the first embodiment. First, as shown in FIG. 6A, the minimum coordinates (Xmin, Ymin) and the maximum coordinates (Xmax, Ymax) existing in the specimen image are automatically detected based on the luminance information arranged in the above two dimensions (step S21). Next, the width and height of the designated segment are calculated from the designated magnification (step S22). The specified magnification can be determined by grabbing the button Sw displayed by the application software with a mouse, or dividing and assigning a function (function) key such as a keyboard of a personal computer. Regarding the calculation of the degree and height of the above-mentioned designated sections, here, the current magnification of the objective lens is used as the basis (read the precautions of the mouth first and then fill out this page) This paper applies the national standard of 4 * ( CNS) A4 size (210 * 297 mm)
• >1 i I cl I I - Hi K— I - ^^1 a— n cl I -1 I I I 1 I 18 經濟部%慧时產局MT工消費合作_社印製 A7 ----^ _B7________ 五、發明說明(l6 ) 準之倍率的比率,予以決定區段大小。即,現在顯示之標 本像的倍率為2倍而欲將其次之指定倍率為4倍之場合,指 定區段之寬度、高度均成為以(640 X 480)所顯示之區段的 一半之大小(320 X 240)。 如是’予以決定指定區段之大小之後,就如在第6B 圖所示’予以決定網目指定之初期位置。即,將前述之最 小座標值予以設定在指定區段之左上’且予作成區段63( 步驟S23) >該區段63之位置乃作為初期位置而予記憶在資 料記憶裝置。而予以記憶中心位置或左左上座標均可 以。 上述初期位置經予決定之後,就如在第6C圖所示, 予以檢出其次之網目位置(步驟S24)。即,將X座標固定而 將Y之移動初期位置作為考濾前述所記憶之區段的高度之 位置’且將指定區段在Y座標方向移動,並予檢出標本像 所存在之處所。 在區段上之X線上至少亦予以辨識1處所之標本存在 處所之場合,就予停止Y方向移動,且以該位置作為其次 之網目位置64,並將該座標記憶在資料記憶裝置(步驟S25) °而再將區段在Y方向移動且予檢出成為其次之網目位置 (步驟S26)。 然後,如在第6D圖所示,若區段之中心座標超過Ymax 之場合(步驟S28),乃將X位置僅予移動第1區段63之寬度 的長度(步驟S29),且將其作為X開始位置,而與上述步驟 S24同樣予以檢出標本所存在之處所(網目位置65至67)。 ί* '泠纥t 用屯a ®家棵A I,C>:S)A4規格(2]0 X 1!97公+餐) 19 ----------裝 ------- 訂 -----1--線 <淆先閱讀背面之注意事項再填寫本頁) 449662 Α7 Β7 經濟部智慧財產局員工消費合作社印製 五、發明說明(17 ) 反覆如上述之作業,而如在第4E圖所示,於區段之 中心座標均超越(Xmax,Ymax)之場合(步驟S28、S30), 即予終了網目指定。 上述網目指定結果,於依賴側之監控器4與觀察側之 監控器2’而將與標本像一起取入之位置指定的區段予以 作線覆蓋顯示。監控器2、4上之網目分割位置資料,乃予 以變換為電動載物台9之座標資料,並以從觀察側之影像 取入要求為依據,而將該電動載物台9移動至各網目分割 指定位置之後,將顯微鏡7解開而以攝影部21(視頻攝影機 能6)取入標本像。 上述監控器上之座標系統變換為電動載物台9之座標 系統,乃以在個人電腦5内之資料記憶裝置事先所記憶之 變換表為根基予以進行。然後,以從個人電腦5對於電動 載物台9所轉送之資料為依據,而予移動電動載物台9。並 且’於電動載物台9之移動後,取入影像並介著未圖示之 線路連接裝置、公用線路3,而將該影像從依賴侧之個人 電腦5對觀察側之個人電腦1作資料轉送β 如上述說明,依據第1實施態樣有關之顯微鏡影像傳 送系統,以簡單之操作,就可去除灰麈等之雜音部份,且 能僅對於標本像自動進行網目分割。 《第2實施態樣》 以下’將說明本發明之第2實施態樣。 於前述之第1實施態樣,上述標本像位置檢出,係將 X座標作為基準而予決定網目分割指定界限,惟,由於標 本紙張尺度適用中國國家標準(CNS)A4規格(210 X 297公爱) 20 (請先閱讀臂面之注項再填寫本頁> \Λ_• > 1 i I cl II-Hi K— I-^^ 1 a— n cl I -1 III 1 I 18 Ministry of Economic Affairs% Huishi Industry Bureau MT Industrial and Consumer Cooperation _Social Printing A7 ---- ^ _B7________ 5. Description of the invention (16) The ratio of the exact magnification shall determine the size of the section. That is, when the magnification of the specimen image currently displayed is 2 times, and the next designated magnification is 4 times, the width and height of the designated segment will be half the size of the segment displayed at (640 X 480) ( 320 X 240). If "the size of the designated section is determined, the initial position of the mesh designation is determined as shown in Fig. 6B". That is, the aforementioned minimum coordinate value is set to the upper left of the designated section 'and a section 63 is created (step S23) > The position of the section 63 is stored in the data storage device as an initial position. It is possible to memorize the center position or the upper left and left coordinates. After the initial position is determined, as shown in FIG. 6C, the next mesh position is detected (step S24). That is, the X coordinate is fixed, the initial position of Y movement is used as the position of the height of the previously memorized segment, and the designated segment is moved in the Y coordinate direction, and the place where the specimen image exists is detected. Where at least one specimen on the X-line on the section is identified as having a specimen, the movement in the Y direction is stopped, and the position is used as the next mesh position 64, and the coordinates are stored in the data storage device (step S25). ) °, and then move the segment in the Y direction and detect it as the next mesh position (step S26). Then, as shown in FIG. 6D, if the center coordinate of the segment exceeds Ymax (step S28), the X position is moved only by the length of the width of the first segment 63 (step S29), and this is taken as X start position, and the place where the specimen exists (mesh position 65 to 67) is detected in the same way as the above step S24. ί * '凌 纥 t 屯 a ® 家 棵 AI, C >: S) A4 size (2) 0 X 1! 97 public + meal) 19 ---------- install -------- -Order ----- 1--line < confusion read the notes on the back before filling in this page) 449662 Α7 Β7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 5. Description of the invention (17) Repeat as described above As shown in FIG. 4E, if the center coordinates of the segment exceed (Xmax, Ymax) (steps S28, S30), the final mesh designation is completed. The above-mentioned mesh designation results are displayed as line overlay on the monitor 4 on the relying side and the monitor 2 'on the observing side, and the segment designated by the position taken in together with the specimen image is displayed. The position data of the mesh division on the monitors 2 and 4 is transformed into the coordinate data of the motorized stage 9, and the motorized stage 9 is moved to each mesh based on the image acquisition requirements from the observation side. After dividing the designated position, the microscope 7 is released and a specimen image is taken in the imaging section 21 (video camera function 6). The conversion of the coordinate system on the above-mentioned monitor into the coordinate system of the motorized stage 9 is performed based on a conversion table stored in advance by a data storage device in the personal computer 5. Then, based on the data transferred from the personal computer 5 to the motorized stage 9, the motorized stage 9 is moved. And 'after the movement of the motorized stage 9, the image is taken and the unconnected line connection device and the public line 3 are taken in, and the image is recorded from the personal computer 5 on the dependent side to the personal computer 1 on the observation side. Transfer β As described above, according to the microscope image transmission system related to the first embodiment, with a simple operation, the noise of gray dust and other parts can be removed, and the mesh segmentation can be automatically performed only on the specimen image. "Second embodiment" Hereinafter, a second embodiment of the present invention will be described. In the aforementioned first implementation aspect, the detection of the specimen image position above uses the X coordinate as a reference to determine the designated mesh division limit. However, since the paper size of the specimen applies the Chinese National Standard (CNS) A4 (210 X 297) Love) 20 (Please read the note on the arm surface before filling in this page > \ Λ_
— — — — — — — I - I 1 I I I I I — — — — — — — — — —— — I I - I I I II II 經濟部智慧时產局員工消費合作.社fRiii A7 ·丨——- ..... _ _ B7 五、發明說明(l8 ) 本亦有以γ基举進行網目分割指定較為良好之場合。考慮 該問題所作者為第2實施態樣,而於本實施態樣,乃以作 為Y基準而予進行網目分割指定為特徵。即,如在第7圖 所示,以網目位置71至76之順序進行網目分割指定。 《第3實施態樣》 現參照第9與10圖之流程圖及第8圖之表示例說明第3 實施態樣。於該第3實施態樣,係將多數之倍率指定為可 能’甚至良交率之網目分割指定為可能。 首先,參照第9圖之流程圖,予以說明第3實施態樣有 關之擴大區段指定處理之順序。 於本順序,乃首先予以自動檢出標本像所存在之位置 的最小座標(Xmin、Ymin)與最大座標(Xmax、Ymax)(步 驟S31)。 接著,依指定倍率而演算指定區段之寬度、高度(步 驟S32)。該指定倍率,係將以應用軟體為依據所顯示之按 鈕SW作抓取而予確立,或者,在個人電腦之鍵盤等之機 能鍵將倍率予以分配而作指定。上述指定區段之寬度、高 度之演算乃以現在之物鏡倍率作為基準之倍率的比率予以 進行。 決定上述指定區段之大小之後,接著,予以決定網目 指定之初期位置。即,將前述之最小座標值設定在指定區 段之左上(步驟S33)。該設定終了之後’就予進行判斷在 左上作相期設定之位置上是否存在標本(步驟S3 4)。 在上述步騍S34,若予判斷有標本存在時’即予算出 21 -----裝--------訂---------線 (请先閱讀背面之注意事項再填寫本頁) 44966 2 Α7 Β7 經濟部智慧时產局員工消費合作社印製 五、發明說明(19 ) 區段面積與區段内之標本像所存在之面積比(步驟§3 5)。 其次’就予判斷區段面積内之標本所存在之面積是否為 50%以下(步驟S36)。於此,若予判斷該面積比為50%以下 ’即’於該區段内可進行更高倍指定之區段處理(區段内 網目處理)(步驟S38) °而有關該區段内網目處理之詳細說 明’將於後述之第10圖的流程圖予以說明。 以區段内網目處理前之現有指定倍率,將區段位置作 為假座標而予記憶在個人電腦5或1内部之未圖示的資料記 憶裝置。 上述高倍率指定區段處理終了之後,就回復為指定倍 率而予檢出其次之網目位置。即,將γ座標予以固定,而 將X移動初期位置從前述之假座標的區段位置,於X座標 方向予以移動於考慮區段寬度之位置(步驟S3 9)。接著, 就予判斷移動後之區段的中心X座標是否超越Xmax(步驟 S41 ),若未超越’就回復為判斷於移動後之區段是否存在 標本(步驟S34)。 另方面,在步驟S36,區段面積與區段内之標本像所 存在之面積比超過50%之場合,就將現有指定倍率之擴大 指定區段作路線顯示’並將該座標記憶在資料記憶裝置( 步驟S37)。 上述座標記錄位置決定之後,乃如在第8圖所示而予 檢出其次之網目位置。即,將Y座標予以固定,而將X之 移動初期位置於X座標方向移動於考慮前述所記憶之區段 的寬度之位置(步驟S39)。 {請先閱讀臂面之注意事項再填寫本頁) 本紙張尺度適用中囡國家攆準(CNS)A4規格(210 X 297公釐) 22 A7— — — — — — — — I-I 1 IIIII — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — II-III II II .. _ _ B7 V. Description of the Invention (l8) There are also occasions when it is better to specify the mesh segmentation with γ-based enumeration. In consideration of this problem, the author is the second embodiment, and in this embodiment, it is characterized by designating the mesh division as the Y standard. That is, as shown in FIG. 7, the mesh division designation is performed in the order of the mesh positions 71 to 76. "Third embodiment" The third embodiment will now be described with reference to the flowcharts in Figs. 9 and 10 and the example shown in Fig. 8. In this third embodiment, it is possible to designate the mesh ratio of the majority as possible, or even the mesh division of the yield ratio. First, with reference to the flowchart in FIG. 9, the procedure of the extended section designation process according to the third embodiment will be described. In this sequence, the minimum coordinates (Xmin, Ymin) and the maximum coordinates (Xmax, Ymax) of the position where the specimen image exists are automatically detected first (step S31). Next, the width and height of the designated segment are calculated at the designated magnification (step S32). The specified magnification is established by grabbing the displayed button SW based on the application software, or by assigning the magnification to a function key such as a keyboard of a personal computer. The calculation of the width and height of the above-mentioned designated section is performed based on the ratio of the current magnification of the objective lens as the reference. After determining the size of the above-mentioned designated section, the initial position of the mesh designation is determined. That is, the aforementioned minimum coordinate value is set to the upper left of the designated area (step S33). After this setting is completed ', it is judged whether or not a specimen exists at the position where the phase is set in the upper left (step S34 4). In the above step S34, if it is judged that there is a specimen, 'it is calculated 21 ----- install -------- order --------- line (please read the note on the back first) Please fill in this page again for details) 44966 2 Α7 Β7 Printed by the Consumers' Cooperative of Wisdom and Time Bureau of the Ministry of Economic Affairs 5. Description of Invention (19) The ratio of the area of the section to the area of the specimen image in the section (step §3 5). Next, it is judged whether or not the area of the specimen within the area of the section is 50% or less (step S36). Here, if it is judged that the area ratio is less than 50%, that is, the segment processing (intra-segment mesh processing) in the segment can be performed at a higher magnification (step S38). The detailed description will be described in the flowchart of FIG. 10 described later. An unillustrated data storage device that stores the position of the segment as a false coordinate and uses the existing specified magnification before mesh processing in the segment is stored in the personal computer 5 or 1. After the processing of the above-mentioned high-magnification designated section is completed, it returns to the designated magnification and the next mesh position is detected. That is, the γ coordinate is fixed, and the initial X-movement position is moved from the position of the aforementioned pseudo-coordinate to the position in the direction of the X-coordinate in consideration of the width of the segment (step S39). Next, it is judged whether the center X coordinate of the segment after the movement exceeds Xmax (step S41), and if it is not exceeded, it returns to determining whether a specimen exists in the segment after the movement (step S34). On the other hand, in step S36, when the ratio of the area of the segment to the area of the specimen image in the segment exceeds 50%, the enlarged designated segment of the existing designated magnification is displayed as a route, and the coordinates are stored in the data memory. Device (step S37). After the above coordinate recording position is determined, the next mesh position is detected as shown in FIG. That is, the Y coordinate is fixed, and the initial moving position of X is moved in the X coordinate direction to a position that takes into account the width of the previously memorized segment (step S39). {Please read the precautions of the arm surface before filling out this page) This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 22 A7
經濟部智慧財產局員工消費合作钍!-!7:食 五、發明說明(2〇) 其次,判斷移動後之區段的中心X座標是否超過最大 座標(Xmax)(步驟S41)。於此*未超過最大座標之場合, 就予判斷於移動後之區段位置是否存在標本(步驟S34)。 於上述步驟S34,於移動後之區段位置,經判斷為未 存在標本之場合,即將Y座標予以固定,且在X方向予以 移動1畫素份區段(步驟S40)。該移動處理,於左上所設定 之初期位置上,未存在標本像之場合亦為同樣意義。 其次,判斷移動1畫素份之區段之後的區段之中心X 座標值,是否超過最大X座標(Xmax)(步骑S4 1)。於此, 未超過之場合,再進行判斷是否存在標本(步驟S34)。該 流程要繼續至有標本存在,或區段移動後之區段中心X座 標超過最大X座標(Xmax)為止。 然後,移動後之區段的中心座標超過最大X座標 (Xmax)之場合(步雜S41),乃將Y位置僅予移動區段之高 度的長度,同時,使X位置為Xmin(步驟S42)。於此所指 定之座標為區段左上之座標。 上述移動後之區段的中心Y座標超過最大γ座標 (Ymax)之場合(步驟S43),即為終了。未超過最大X座標 之場合’為決定其次之網目位置,乃回復為在移動後之區 段位置是否存在標本之判斷處理(步驟S34)。 其次,參照第10圖之流程圖’釺對於第9圖内,區段 内之標本像所存在之面積比為50%之場合,對區段内作更 南倍率指定區段指定處理(以下’稱為區段内網目指定)作 說明= ------------*裝-------訂-----I---線 f請先閱讀背面之注意事項再填寫本頁} -23 - 449662 Α7 Β7 經濟部智慧財產局員工消费合作社印製 五、發明說明(21 ) 首先,予以判斷在區段内是否存在能作更高倍率指定 之同樣物體(步驟S51)。若現區段指定倍率非為最高倍率 ,即執行處理,若不然,即予終了處理。 於上述步驟S5 1,判斷為比現區段指定倍率有更高之 倍率的場合,就予檢出區段内之標本像的最小、最大座標 (Xmin、Ymin)、(Xmax、Ymax)(步驟S52)。 其次’演算比現區段指定倍率為更高倍之擴大指定區 段的寬度與高度(步驟S53)。例如,於顯微鏡有裝置ι.25χ 、2x、4x、10x、20x、40x之物鏡’而現區段指定倍率為2χ 之場合’將更高倍之物鏡設定為4χ,而予演算在區段上之 更高倍的擴大區段寬度與高度》 接著’將區段内之更高倍的擴大指定區段之初期位置 予以設定在區段内之左上(步驟S54)。然後,予以判斷於 該初期位置是否存在標本(步驟S55)。 於此,有標本存在時,就予算出初期區段面積與區段 内之標本像所存在之面積比(步驟S56)。 其次,予以判斷面積比是否為50%以下(步驟S57)。 於此’若為50%以下,就再作更高倍之指定區段處理(區 段内網目處理)(步驟S59)。區段内網目處理,乃予進行至 指定物鏡超過最大倍率為止。於此例,即,將區段網目繼 續至最大倍率為止,惟,以任意之倍率作為上限予以進行 區段内網目處理亦可以。 而以區段内網目處理前之現指定倍率,而將區段位置 作為假座標予以記錄在資料記憶裝置。當區段内網目處理 本紙張尺度適用中國國家標準(CNS)A4規格(210x297公爱) 24 (諳先閱讀背面之注意事項再填寫本頁) -n n I I ϋ ϋ 如5, n I I ϋ n ϋ I I I I ϋ If ϋ I ϋ I n / ϋ I ϋ ϋ n I 1 A7 B7 經濟部智慧財產局員工消費合泎.吐.印製 五、發明說明(22) 终了後,恢復為指定倍率,並予檢出其次之網目位置。即 ’將X座標固定’而將γ移動初期位置作為從前述之假座 標的區段位置予以考慮區段高度之位置,而予移動於γ座 標方面(步驟S60)。然後’判定移動後之區段的中心γ座標 是否超過Ymax(步驟S62),若未超過,就恢復為判斷移動 後之區段的標本是否存在(步驟S55)。 另方面’若區段面積與區段内有標本像存在之面積比 為超過50%之場合,就予顯示在區段内有再高倍之線列, 並予記憶座標與此時之指定對物倍率(步驟S58)。為檢出 其次之區段内網目位置’乃將X固定,且作為考慮γ移動 後之區段的高度之位置’而在γ座標方向予以移動(步驟 S60)。該移動後之區段中心γ座標若未超過Yrnax(步驟S62) ,即,恢復為判斷於移動後之區段是否存在標本(步驟S55) 〇 在上述步驟S55 ’經予判斷是否有標本存在之結果, 若標本不存在之場合,乃將X固定,而在γ方向予以移動j 畫素份區段内之區段(步驟S61)。 然後,區段内之移動區段的中心座標Y座標超過Ymax 之場合(步驟s62)’乃將X位置僅予移動區段内之移動區段 的寬度之長度,同時,將Y位置設為Ymin(步驟S63)。 並且,予以判斷區段内之移動區段的中心X座標是否 超過最大Xmax(步驟S64)。於此,中心X座標超過最大xmax 之場合,即,區段内網目處理就終了。又,區段内之移動 區段的中心X座標未超過最大Xmax之場合,就恢復為是 夂ΐ由驾國家嘌擞(C\-S>A4規烙(210 X 297公釐〉 ---I---------裝·!-----訂 --------線 (請先閱讀肯面之ί£意事項再填窵本頁) 經濟部智慧財產局員Η消費合作社印製 449662 Α7 __ Β7 五、發明說明(23 ) 否存在標本之判斷處理(步驟S55),並將前述處理繼績至 移動區段之中心座標超過Xmax、Ymax為止。 如在上述說明,依據第3實施態樣有關之顯微鏡影像 傳送系統,能以區段内之標本像的占有率為依據,以自動 進行更適宜之網目分割。 《第4實施態樣》 其次,說明本發明之第4實施態樣該第4實施態樣, 係辨識標本像位置之方法乃以使用色資訊為其特徵。即, 一般上,病理標本依染色法而成為紅系之顏色,或染成藍 系之顏色’惟,於第4實施態樣,由於檢出有關標本像之 染色資訊,予以辨識標本像位置。 在構成上,將僅予柚出紅的空間頻率領域之第〗感測 器’僅予抽出藍的空間頻率領域之第2感測器,僅予抽出 綠的空間頻率領域之第3感測器,設在顯微鏡7内方面乃與 第ί實施態樣相異。在各感測器,由於以視頻攝影機6内之 光束分離器可使標本像予以分割且予引導,因此,各感測 器之位置乃予配置在與視頻6攝影機能為光學性之同位置上。 上述第1至第3之感測器的輸出乃以畫素單位予以抽出 ,而該等乃予記錄在個人電腦内之資料記憶裝置。而且, 將第1至第3感測器之輸出接近於最大值之位置,予以辨認 為背景位置,而將第1至第3感測器之輸出為接近於最低值 之位置’予以辨識為不要之資料位置,且僅予抽出紅之空 間頻率領域而僅有從第1感測器之輸出,成為某臨限值以 上之位置予以辨識為標本像位置。而由於染色法亦有僅予 本纸張尺度適用中國國家標準(CNS)A4規格(210 X 297公釐) 26 -------------Li----— —訂---------線丨 t: <請先Μ讀背面之注意事項再填寫本頁) A7 A7 經漘部智慧財產局3工消費合作社卬^ _______B7__ 五、發明說明(24 ) 抽出藍之空間頻率領域且僅有從第2感測器之輸出,成為 某臨限值以上之位置作為標本像位置。 而依色資訊作標本像位置辨識方面,使用上述第1至 第3感測器之外,使用於顯微鏡7内僅將分別對應於红、綠 、藍之空間頻率予以通過之光學片光鏡,亦為檢出可能。 即,對於1個人感測器,進行顯微鏡7之光學濾光鏡的切換 ,且將僅為目的之空間頻率領域之像引導至視頻攝影機6 ,並由所獲得之影像資訊而予辨識標本像所存在之位置, 且亦能予記錄在資料記憶裝置。 而於上述實施態樣所使用之感測器方面,乃以2次元 面積感測器為佳’惟,並未作特別之限定。又於上述實施 態樣,乃使用第1至第3感測器而將紅、綠、藍之區間頻率 全部觀看’但’並不限定於此,例如,僅以對應於較易辨 識形狀態之綠的的空間頻率之感測器,亦可辨識背景位置 ’不要之資料位置,標本位置。 如上述說明’依據第4實施態樣有關之顯微鏡影像傳 送乐統’依據標本像之色資訊亦能予進行高精度之網目分 割。 《第5實施態樣》 其次,說明本發明之第5實施態樣。 於則述之第1至第3實施例,係予表示以自動作網目分 割之例。對其,第4實施態樣,係將操作者一度自動所破 定之網目分割位置,由於操作在個人電腦之監控器上所顯 示之網目區段,就能予任意移動、削除、追加,為其特 、3同國家標度(CNS)A4 規恪(21ϋχ297 公.¾ -----------1 I I ---------------線 <請先閲璜背面之注$項再填寫本頁) 27 449662Consumer cooperation of the Intellectual Property Bureau of the Ministry of Economic Affairs!-! 7: Food V. Invention description (20) Secondly, it is judged whether the center X coordinate of the moved section exceeds the maximum coordinate (Xmax) (step S41). When the maximum coordinate is not exceeded here, it is judged whether a specimen exists at the position of the section after the movement (step S34). In the above step S34, if it is determined that the specimen does not exist at the position of the section after the movement, the Y coordinate is fixed and the section is moved by 1 pixel in the X direction (step S40). This moving process has the same meaning when there is no specimen image at the initial position set on the upper left. Next, it is judged whether the value of the center X coordinate of the section after the section moved by 1 pixel portion exceeds the maximum X coordinate (Xmax) (step riding S4 1). In this case, if it is not exceeded, it is determined whether or not a specimen exists (step S34). The process continues until a specimen exists or the X coordinate of the center of the segment after the segment moves exceeds the maximum X coordinate (Xmax). Then, if the center coordinate of the moved segment exceeds the maximum X coordinate (Xmax) (step S41), the Y position is only given the length of the height of the moved segment, and the X position is set to Xmin (step S42) . The coordinates specified here are the coordinates on the upper left of the segment. When the center Y coordinate of the above-mentioned moved section exceeds the maximum γ coordinate (Ymax) (step S43), it is the end. When the maximum X coordinate is not exceeded 'is to determine the next mesh position, and it is returned to the judgment processing of whether or not a specimen exists in the segment position after the movement (step S34). Next, refer to the flowchart in FIG. 10 '釺 For the case where the area ratio of the specimen image in the section in FIG. 9 is 50%, the section designation processing for the souther magnification designation in the section (the following is performed) (Referred to as the mesh designation in the section) for explanation = ------------ * installation ------- order ----- I --- line f Please read the note on the back first Please fill in this page again} -23-449662 Α7 Β7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of Invention (21) First, determine whether there is the same object in the section that can be specified at a higher magnification (step S51). If the specified magnification of the current section is not the highest magnification, the processing is executed; otherwise, the processing is terminated. When it is judged that the magnification is higher than the designated magnification of the current segment in step S51, the minimum and maximum coordinates (Xmin, Ymin), (Xmax, Ymax) of the specimen image in the segment are detected (step S52). Next, the width and height of the enlarged designated area are calculated at a higher magnification than the designated magnification of the current segment (step S53). For example, if the microscope has an objective lens of 25x, 2x, 4x, 10x, 20x, and 40x, and the magnification of the current segment is specified as 2x, a higher magnification objective is set to 4x, and the value calculated on the segment Higher Width and Height of Enlarged Segment> Then, 'the initial position of the enlargement of the specified segment within the segment is set to the upper left of the segment (step S54). Then, it is determined whether a specimen exists at this initial position (step S55). Here, when a specimen exists, the ratio of the area of the initial segment to the area of the specimen image in the segment is calculated (step S56). Next, it is determined whether the area ratio is 50% or less (step S57). Here, if it is 50% or less, the designated segment processing (the mesh processing in the segment) is performed at a higher magnification (step S59). Mesh processing in the section is performed until the specified objective lens exceeds the maximum magnification. In this example, the segment mesh is continued up to the maximum magnification, but it is also possible to perform mesh processing in the segment with an arbitrary magnification as the upper limit. The current magnification before mesh processing in the segment is used, and the position of the segment is recorded as a false coordinate in the data storage device. When the mesh in the section is processed, the paper size applies the Chinese National Standard (CNS) A4 specification (210x297 public love) 24 (谙 Please read the precautions on the back before filling this page) -nn II ϋ ϋ If 5, n II ϋ n ϋ IIII ϋ If ϋ I ϋ I n / ϋ I ϋ ϋ n I 1 A7 B7 Consumption of employees in the Intellectual Property Bureau of the Ministry of Economic Affairs. Spit. Printed 5. Description of the invention (22) After the end, it will be restored to the specified rate and inspected. Next is the mesh position. That is, "the X coordinate is fixed", and the initial position of the? Movement is taken as the position in which the segment height is taken into consideration from the aforementioned segment position of the false coordinate, and is moved to the? Coordinate (step S60). Then, it is determined whether the center γ coordinate of the segment after the movement exceeds Ymax (step S62), and if not, it is restored to judge whether the specimen of the segment after the movement exists (step S55). On the other hand, if the ratio between the area of the section and the area where the specimen image exists in the section is more than 50%, it will be displayed in the line with a higher magnification in the section, and the coordinates and the designated object at this time will be memorized. Magnification (step S58). In order to detect the mesh position 'in the next segment', X is fixed and moved in the direction of the γ coordinate as the position of the height of the segment after γ's movement is considered (step S60). If the γ coordinate of the center of the segment after the movement does not exceed Yrnax (step S62), that is, it is restored to determine whether a specimen exists in the segment after the movement (step S55). In the above step S55, it is determined whether a specimen exists. As a result, if the specimen does not exist, X is fixed, and the section within the j pixel section is moved in the γ direction (step S61). Then, when the center coordinate Y of the moving section in the section exceeds Ymax (step s62) ', the X position is only given to the width of the moving section in the moving section, and the Y position is set to Ymin (Step S63). Then, it is determined whether the center X coordinate of the moving section in the section exceeds the maximum Xmax (step S64). Here, when the center X coordinate exceeds the maximum xmax, that is, the mesh processing in the section is ended. In addition, if the center X coordinate of the moving section in the section does not exceed the maximum Xmax, it will be restored to the national standard (C \ -S > A4 (210 X 297 mm>) --- I --------- Equipment !! ----- Order -------- line (please read the meaning of Ken Noodle before filling out this page) Member of Intellectual Property Bureau, Ministry of Economic Affairs印 Printed by a consumer cooperative 449662 Α7 __ B7 V. Description of the invention (23) The judgment processing of the existence of the specimen (step S55), and the foregoing processing will be continued until the center coordinates of the moving section exceed Xmax, Ymax. As described above According to the microscope image transmission system related to the third embodiment, a more appropriate mesh segmentation can be performed automatically based on the occupancy ratio of the specimen image in the section. "Fourth embodiment" Next, the invention will be described. Fourth implementation aspect The fourth implementation aspect is a method of identifying the position of the specimen image by using color information as its characteristic. That is, in general, pathological specimens are colored by the red system or stained by the blue system. The color is "but in the fourth embodiment, the specimen was identified because the staining information about the specimen image was detected. In terms of structure, only the second sensor in the spatial frequency domain where red is extracted from grapefruit will be used. The second sensor will only be extracted in the spatial frequency domain where blue will be used, and the third sensor will only be extracted in the green spatial frequency domain. The detector is different from the first embodiment in that it is provided in the microscope 7. In each sensor, the specimen image can be divided and guided by the beam splitter in the video camera 6. Therefore, each sensor The positions of the sensors are arranged at the same positions as those of the video 6 camera. The outputs of the sensors 1 to 3 above are extracted in pixel units, and these are recorded in the personal computer. The data memory device. Also, the position where the output of the first to third sensors is close to the maximum value is recognized as the background position, and the output of the first to third sensors is the position close to the lowest value. 'Identified as an unnecessary data position, and only extracted the red space frequency range and only the output from the first sensor, the position above a certain threshold is identified as the specimen image position. Because of the staining method also Available for this paper only With China National Standard (CNS) A4 specification (210 X 297 mm) 26 ------------- Li ----— -Order --------- line 丨 t : < Please read the notes on the back before filling in this page) A7 A7 Ministry of Economics and Intellectual Property Bureau, 3 Industrial Consumer Cooperatives _______B7__ V. Description of Invention (24) The blue space frequency field is extracted and only from the second The output of the sensor becomes a position above a certain threshold value as the specimen image position. For the identification of the specimen image position according to the color information, in addition to the first to third sensors described above, it is only used in the microscope 7 It is also possible to detect optical lenses that pass through the spatial frequencies of red, green, and blue, respectively. That is, for one personal sensor, the optical filter of the microscope 7 is switched, and the image of the spatial frequency domain for the purpose is guided to the video camera 6 and the specimen image is identified from the obtained image information. Existing location and can also be recorded in the data memory device. In terms of the sensors used in the above embodiments, a two-dimensional area sensor is preferred, but it is not particularly limited. In the above implementation aspect, the first, third, and third sensors are used to view all the frequencies of the red, green, and blue sections. However, the frequency is not limited to this. For example, only the signals corresponding to the more easily recognizable states are used. The green spatial frequency sensor can also recognize the background position 'unwanted data position, specimen position. As described above, 'Transferring Microscope Images Related to the 4th Implementation Aspect' can also perform high-precision mesh segmentation based on the color information of the specimen image. "Fifth Embodiment" Next, a fifth embodiment of the present invention will be described. The first to third embodiments described below are examples of automatic mesh division. In contrast, the fourth embodiment is to automatically divide the mesh division position once determined by the operator. Since the mesh segment displayed on the monitor of the personal computer is operated, it can be arbitrarily moved, deleted and added. Special, 3 with national scale (CNS) A4 regulations (21ϋχ297 mm. ¾ ----------- 1 II --------------- line < please (Please read the note $ on the back of the book before filling out this page) 27 449662
五、發明說明(25) 以下,參照第11圖之流程圚及第1 2圖之表示例,予以 說明依第5實施態樣之網目區段的移動操作。 首先,將在經予作網目分割之區段之中,欲予移動之 任意的區段,於監控器2之畫面上予以操作滑鼠等而作抓 取(步驟S101) 〇 個人電腦1就予辨識該滑鼠位置座標,且將該座標予 以β己憶在個人電腦5或1内部之未囷示的資料記憶裝置内( 步驟S102卜然後,將已經記錄完成之網目分割座標資訊 由資料記憶裝置取出(步驟s 103)。 由於該滑鼠位置與網目座標位置資料,乃予判斷在滑 鼠抓取位置是否存在網目座標位置(步驟S丨〇4)。該滑鼠之 抓取位置’若在指定區段之中,即,具有押住區段内之其 面積(範圍)亦可辨識之判斷機能。 現於滑鼠抓取位置已有網目區段存在時(步驟sl〇5), 並予辨識為將其欲予移動之網目。使滑鼠作抓取之原狀由 於將滑鼠予以移動(步驟s 106),且予變更網目顯示位置( 步驟S107)。上述網目位置之變更,即將網目線列之消除 ,作成反復予以進行。 而網目分割位置之任意的處所的削除,乃予判斷在滑 鼠抓取位置是否存在網目分割座標資訊,並依削除裝置予 以進行。削除裝置,以鍵盤上之留意的按鈕,例如,以「 Delete」鍵亦可,或使用由應用軟艎所表示之削除按紐亦 可以。 28 {請先閱讀臂面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 本紙張尺度適用中國國家標準(CNS)A4規格(210 X 297公爱)V. Description of the invention (25) Hereinafter, referring to the flow chart in FIG. 11 and the example of the illustration in FIG. 12, the movement operation of the mesh section according to the fifth embodiment will be described. First, among the segments divided by the meshes, any segment to be moved is operated on the screen of the monitor 2 by using a mouse or the like to capture it (step S101). Identify the position coordinates of the mouse, and store the coordinates in the unshown data storage device inside the personal computer 5 or 1 (step S102). Then, the recorded coordinate information of the mesh division is saved by the data storage device. Take out (step s103). Due to the mouse position and mesh coordinate position data, it is determined whether there is a mesh coordinate position at the mouse grabbing position (step S 丨 〇4). The grabbing position of the mouse is In the designated section, that is, it has the judgment function that its area (range) in the hold section can be recognized. When the mesh section already exists in the mouse grabbing position (step S105), It is recognized as the mesh to be moved. The original state of the mouse is to move the mouse (step s 106) and change the display position of the mesh (step S107). The change of the above mesh position is the mesh line Eliminated, The creation is performed repeatedly. The erasing of any place in the mesh division position is to determine whether there is mesh division coordinate information at the mouse grabbing position and perform it according to the deletion device. The deletion device uses the attention button on the keyboard. For example, you can use the "Delete" key, or you can use the delete button indicated by the application software. 28 {Please read the precautions on the arm surface before filling out this page) Printed by the Intellectual Property Bureau's Consumer Cooperatives This paper size applies to China National Standard (CNS) A4 (210 X 297 public love)
-^1 ^1 ^1 ϋ I— ^-eJ It n I I n n ti n I-^ 1 ^ 1 ^ 1 ϋ I— ^ -eJ It n I I n n ti n I
五、發明說明(26) 網目分割位置之追加’係予判斷在滑鼠抓取位置是否 存在網目分割座標資訊。然後,未存在時,有可能新規予 以指定網目區段,而於滑鼠抓取之時,將新規網目分割位 置座標予以記錄在資料記憶裝置。如上述,一度曾以自動 進行網目分割指定之後,操作者能任意作網目分割指定之 移動、追加、削除。 如上述說明,依據第5實施態樣有關之顯微鏡影像傳 送系統,以自動進行網目分割之後,乃在所望之位置而能 將區段作適宜移動。即’觀察之自由度(彈性)就可提升。 《第6實施態樣》 其次,將說明本發明之第6實施態樣。 於第6實施態樣,係以自動辨識網目之自動調焦位置 ’作為特徵。以網目分割指定取入影像之場合,即,將焦 點位置固定為1處所而予取入多數之網目分割位置之影像 〇 以下’參照第13圖之流程圖及第14圖之表示例予以說 明依第6實施態樣之自動調焦位置辨識動作。 以影像之明暗比資訊為依據而予決定焦點位置之場合 ’明暗度差愈大之影像愈能作正確之自動調焦。以自動檢 出網目分割位置之時’首先,在中心座標附近將有標本像 之網目分割位置資訊’與各區段内之最大輝度準位差予以 言己億在個人電腦5或1内部之未圖示的資料記情裝置。在中 心座標附近是否存在標本像’乃能將前述標本像辨識用資 料而從資料記憶裝置讀出且予使用就可判斷。 ------------- 裝------- 訂·--------線 (請先閱讀背面之注帝?事項再填寫本頁) 29 449662 經濟部智慧財產局員工消費合作狂印製 A7 B7 五、發明說明(27 ) 即,VGA顯示之場合’以g(320,240)作為中心,而 能由任意之畫格區段(例如’ 50畫格X50畫格)之輝度資訊 是否為0(標本存在)而予判斷(步驟S51)。該區段以平均化 之輝度資訊亦可以》選擇在影像之令心附近有標本像之區 段’乃因作自動調焦之場合,顯微鏡之自動調焦用線列感 測器要取得中心附近之資料之故。 柚出在影像之中心附近有標本像存在之網目區段,且 將該區段位置格納在資料記憶裝置上。 其次,在影像之中心附近,於有存在標本像之所有的 網目各區段之中予以抽出有最大輝度差之區段(步驟S52) 。然後’檢出具有最大輝度差之網目分割位置,且將該位 置作為欲作自動調焦之處所而予記憶在資料記憶裝置(步 驟S53)。於本實施態樣’乃使網目分解位置ι〇1予以選定 為作自動調焦之處所。 實際上,於取入網目分割影像之時,首先,將電動載 物台9予以移動至作該自動調焦之處所,且由個人電腦5側 予控制而使其於該位置進行顯微鏡7之自動調焦控制。 如上述說明,依據第6實施態樣有關之顯微鏡影像傳 送系統’乃以網目分割之結果為依據,而以簡單之操作, 能以高精度自動辨識焦點位置。 《第7實施態樣》 其次’說明本發明之第7實施態樣。 一般上’宏觀像會發生周邊光量不足之情形(參照第15 圖),而該周邊光量不足將使輝度準位降低。由此,於素 本紙張尺度適用中國國家標準(CNS)A4規格(210x297公« ) 30 (請先閱讀't面之注意事項再填寫本頁) -n ϋ ϋ ^eJI ϋ u I n I n I— n n If n ϋ n n _ A7 — B7 五、發明說明(28) 來之自動網目分割位置辨識算法,乃依據輝度資訊進行處 I_______I____裝 I — (請先間讀臂面之注意等項洱填寫本頁> 理,因此,有可能因周邊光量不足之影響而不能作正確之 網目分割指定。 即,發生周邊光量不足之時,將本來作為背景而應予 以辨識之資訊有可能予以誤辨識為標本像之輝度準位,因 此,於背景之部份亦會塗蓋網目分割位置。於第7實施態 樣,乃對於發生該周邊光量不足之標本像進行適正之網目 分割指定作為特徵。 以下,參照第16圖之流程圖,詳細說明對於發生周邊 光量不足之標本的網目分割指定之動作。 -線 首先’實際上將標本像作為資料而予辨識之前,以無 標本之狀態取入影像,而將該影像資訊記錄在資料記憶裝 置或M0等之記錄裝置。於本實施態樣,即以^0(640,48〇) 、GO(640、480)、BO(640、480)之2次元的排列資料予以 格納在資料記憶裝置(步驟S61)。此場合,由於攝影背景 資訊,因此’影像就成為白色,而全部畫素之輝度資訊應 均為FF(255),惟’在周邊部份之座標位置就不成為ff(255) 經濟部智慧时臺局員工消費合作.吐-.^ 因此,為格納於2次元排列之時,就對於所有之畫素 資訊進行如下之演算。 R0(x,y)=FF(255)-R0(x,y) (x== 1〜640、y = 1 〜480) G0(x,y)=FF(255) — G0(x,y) (x = 1 〜640 ' y = 1 〜480) 31 449662 A7 B7 五、發明說明(29 ) BO(x,y)=FF(255)-B0(x,y) (x= 1 〜640、y= 1 〜480) 即,將於周邊光量不足之畫素部份應予加算之輝度資 訊 ’ s己憶在R0(640、480)、G(640、480)、B(640、480)。 接著’將宏觀像之原像取入’且將影像資訊予以格納 在 Rl(640、480)、Gl(640、480)、Bl(640、480)之2次元 的排列記憶體(步驟S62)。從原像之影像資訊加算無標本 像之影像資訊’並將資料作為R(640、480)、G(640 ' 480) 、B(640、480)。於此,各每一影像進行加算(步驟S63)。 詳細為: R(x,y)=Rl(x,y)+R0(x,y) (x= 1 〜640、y= 1 〜480) G(x,y)=Gl(x,y)+G0(x,y) (x= I 〜640、y= 1 〜480) B(x,y)=Bl(x,y)+B0(x,y) (x= 1 〜640、y= 1 〜480) 如上述說明,依據第7實施態樣有關之顯微鏡影像傳 送系統,就能僅對周邊光量不足之部份作資料之修補。即 ,於第7實施態樣,以修補過周邊光量不足之資料之結果 作為根基,而予進行前述網目分割指定,就可自動作更正 確之網目分割指定。 《第8實施態樣》 其次,說明本發明之第8實施態樣》該第8實施態樣, 係予抽出色資訊,且使用該資訊予以檢出標本像位置為其 本紙張尺度適用中國國家標準(CNS>A4規格(210 X 297公釐) (請先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作钍印製V. Explanation of the invention (26) Addition of mesh segmentation position 'is to judge whether there is mesh segmentation coordinate information at the mouse grabbing position. Then, if it does not exist, it may be possible to specify a new mesh section in the new rule, and when the mouse grabs it, the coordinate position of the new ruled mesh is recorded in the data storage device. As mentioned above, once the mesh division designation was performed automatically, the operator could arbitrarily move, add, or delete the mesh division designation. As described above, according to the microscope image transmission system related to the fifth embodiment, after the mesh is automatically divided, the segment can be moved appropriately at the desired position. That is, the degree of freedom (elasticity) of observation can be improved. "Sixth embodiment" Next, a sixth embodiment of the present invention will be described. In the sixth embodiment, it is characterized by automatically recognizing the automatic focus position of the mesh. In the case of designating an image to be taken by mesh division, that is, to fix the focus position to one place and take in a large number of mesh division positions, the following description will be described with reference to the flowchart in FIG. 13 and the representation example in FIG. 14. The sixth embodiment performs an automatic focus position recognition operation. When the focus position is determined based on the image's light-dark ratio information ’The larger the light-dark difference is, the more accurate auto-focus can be made. When the mesh segmentation position is detected automatically, "First, the mesh segmentation position information with the specimen image near the center coordinates" and the maximum brightness level difference in each section are used to indicate the number of points within the personal computer 5 or 1. Pictured data recording device. Whether or not a specimen image exists near the center coordinates can be determined by reading the aforementioned specimen image identification data from a data storage device and using it. ------------- Install ------- Order · -------- Line (Please read the Note on the back? Matters before filling out this page) 29 449662 Economy Ministry of Intellectual Property Bureau employee cooperation prints A7 B7 V. Invention Description (27) That is, in the case of VGA display 'g (320, 240) as the center, and can be from any frame segment (such as '50 pictures Frame X50 frame) to determine whether the luminance information is 0 (specimen exists) (step S51). This section can also use the averaged luminance information. "Select the section with specimen images near the center of the image 'for the purpose of automatic focusing. The line sensor for the automatic focusing of the microscope must be near the center. The reason for the information. Pomelo comes out of the mesh section where the specimen image exists near the center of the image, and the location of this section is on the data memory device. Next, the segment with the largest luminance difference is extracted from all the segments of the mesh where the specimen image exists near the center of the image (step S52). Then, the mesh division position having the largest luminance difference is detected, and this position is stored in the data storage device as a place to be automatically adjusted (step S53). In this embodiment mode, the mesh decomposition position ι〇1 is selected as a place for automatic focusing. Actually, when the mesh-divided image is acquired, first, the motorized stage 9 is moved to the place where the automatic focusing is performed, and controlled by the personal computer 5 side so that the microscope 7 is automatically performed at the position. Focus control. As described above, the microscope image transmission system 'according to the sixth embodiment is based on the results of mesh segmentation, and with a simple operation, it can automatically identify the focus position with high accuracy. "Seventh embodiment" Next, a seventh embodiment of the present invention will be described. Generally, a macro image will have insufficient peripheral light (see Figure 15), and the insufficient peripheral light will reduce the brightness level. Therefore, the Chinese standard (CNS) A4 specification (210x297 male «) is applicable to the standard paper size. 30 (Please read the precautions on the 't side before filling out this page) -n ϋ ϋ ^ eJI ϋ u I n I n I— nn If n ϋ nn _ A7 — B7 V. Invention description (28) The automatic mesh segmentation position recognition algorithm is based on the luminance information. I_______I____install I — (Please read the attention of the arm surface first.) Fill in this page for the reason. Therefore, due to the influence of insufficient ambient light, you may not be able to specify the correct mesh division. That is, when insufficient ambient light occurs, the information that should be identified as the background may be misidentified. It is the brightness level of the specimen image, so the mesh segmentation position is also covered on the background. In the seventh implementation aspect, the proper mesh segmentation designation is performed on the specimen image where the peripheral light quantity is insufficient. The following features With reference to the flowchart in Fig. 16, the detailed description of the mesh division designation of the specimen with insufficient peripheral light quantity will be described.-The line first "actually recognizes the specimen image as data, The state of the specimen is taken into the image, and the image information is recorded in a data storage device or a recording device such as M0. In this embodiment, ^ 0 (640, 48〇), GO (640, 480), BO ( 640, 480) of the two-dimensional permutation data is given to Genna in the data storage device (step S61). In this case, because of the background information of the photography, 'the image becomes white, and the luminance information of all pixels should be FF (255) However, the position of coordinates in the surrounding part will not become ff (255). The staff of Taiwan Bureau of the Ministry of Economic Affairs will cooperate with consumer spending. Tu-. ^ Therefore, when it is arranged in the 2nd dimension, it will be performed for all pixel information. The calculation is as follows: R0 (x, y) = FF (255) -R0 (x, y) (x == 1 ~ 640, y = 1 ~ 480) G0 (x, y) = FF (255) — G0 ( x, y) (x = 1 to 640 'y = 1 to 480) 31 449662 A7 B7 V. Description of the invention (29) BO (x, y) = FF (255) -B0 (x, y) (x = 1 (~ 640, y = 1 to 480) That is, the luminance information that should be added to the pixel portion with insufficient peripheral light quantity is stored in R0 (640, 480), G (640, 480), B (640, 480). Then 'take the original image of the macro image' and the image information It is stored in the 2-dimensional array memory of R1 (640, 480), Gl (640, 480), and Bl (640, 480) (step S62). The image information of the original image is added to the image information without the specimen image and The data is R (640,480), G (640'480), B (640,480). Here, each image is added (step S63). The details are: R (x, y) = Rl (x, y) + R0 (x, y) (x = 1 to 640, y = 1 to 480) G (x, y) = Gl (x, y) + G0 (x, y) (x = I to 640, y = 1 to 480) B (x, y) = Bl (x, y) + B0 (x, y) (x = 1 to 640, y = 1 to 480) As described above, according to the microscope image transmission system related to the seventh embodiment, the data can be repaired only for the insufficient part of the surrounding light. That is, in the seventh embodiment, based on the result of repairing the data with insufficient peripheral light quantity as the basis, and performing the aforementioned mesh segmentation designation, a correct mesh segmentation designation can be made automatically. "Eighth Implementation Aspect" Next, the eighth implementation aspect of the present invention will be described. The eighth implementation aspect is to extract excellent information and use the information to detect the specimen image position for its paper size. Applicable to China Standard (CNS > A4 specification (210 X 297 mm) (Please read the precautions on the back before filling out this page) Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs for consumer cooperation
I·J n .^1 n I f 1_ n n *1 MmM. t— n I 32 經濟部智慧財產局員工消費合泎社印敦 A7 ______Β7________ 五、發明說明(3〇 ) 特徵在之顯微鏡影像傳送系統。 於此,第17圖係表示於本實施態樣,依抽出色資訊而 予檢出標本像位置之過程的模式圖,第18圖係表示有關本 實施態樣之顯微鏡影像傳送系統的機能方塊圖。第丨9圖、 第20圖係欲予說明本實施態樣有關之顯微鏡影像傳送系統 之動作的流程圖。 如在第18圖所示,該顯微鏡影像傳送系統,係可大別 為影像攝入部500、内部處理部5〇 1、使用者操作者5〇2、 及顯微鏡503。 於上述影像取入部500,乃予配設定視頻攝影機部2〇 j 與視頻率捕捉部202。於上述顯微鏡503,乃予配設自動調 焦部203、倍率切換部204、及載物台移動部2〇5。 在上述内部處理部5 01 ’有配設欲予記憶顯示影像之 影像顯示記憶體206,欲予控制視頻捕捉部2〇2之視頻捕捉 控制器207、標本像位置辨識處理部214、記憶體300,欲 予以控制外部記憶記憶體218之外部記憶記憶體控制部214 ’欲予控制自動調焦部203之焦點控制部215,欲予控制倍 率切換部204之倍率控制部216,以及欲予控制載物台移動 部205之載物台控制部217。並且,在上述記憶體300,乃 有各輝度記憶體209至211、RG輝度差記憶體212、BG輝 度差記憶體213、RG輝度差最大值記憶體225、BG輝度差 最大值記憶體226、RG臨限值記憶體227 ' BG臨限值記憶 體;!28之各領域。 在上述使用者操作部502,乃分割別予以配設定顯示 S 因家揉 gt("cN-S>Al 規格(210x297 公S ) !^~~" -------------裝--------訂---------線 (請先閱讀-f面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 449662 Α7 __Β7 五、發明說明(η) 上述影像顯示記憶體206之影像的影像顯示部219、標本像 位置辨識指定部220 *影像保存/呼叫指示部,由上述焦 點控制部215而予指定焦點控制之焦點指定部222,由上述 倍率控制部216而予指定倍率控制之倍率指定部223,以及 由上述載物台控制部217而予指定載物台控制之載物台位 置指定部224。 以下’說明依有關構成所實現之標本像位置檢出之過 程。於本實施態樣’將顯微鏡下之標本像介著視頻攝影機 能部201予以取入,且於視頻率捕捉部2〇2變換為數位資料 ’並將該數位資料取入於影像顯示記憶艘206之後,作為 靜止影像資訊而顯示於影像顯示部219。該標本像之樣子 乃表示在第17Α圖》 而在該第17Α圊所示之表示例,乃在標本像之領域以 外有混存顯微鏡用載玻璃上之蓋玻璃片的影子或灰塵等之 領域。 將在該第17A囷之標本像的任意之γ座標位置上的輝 度資訊’作色分離而所顯示之樣子’乃予表示在第17Β圖 。背景部份及雜音部份,其紅、綠之輝度資訊之變化乃予 推測為同樣,而於有標本存在之位置,即於紅與綠之輝度 資訊產生差值。一般上,病理標本影像,為作觀察而作種 種之染色,因此,使用該輝度差就能予算出標本像位置。 於該第17圊之例,將在整體以紅主體所染色之病理標本作 為對象,惟,由於由染色成為特徵之色資訊乃相異β因此 ,各輝度資訊方面就予取得紅、綠 '藍之3種資訊。 (請先閱讀脅面之注意事項再填寫本頁) -I i n n 』SJI .1 n n ϋ I I I I _ ϋ I [ ϋ n I* nI · J n. ^ 1 n I f 1_ nn * 1 MmM. T— n I 32 Staff Consumption Cooperative of Intellectual Property Bureau, Ministry of Economic Affairs, India A7 ______ Β7 ________ 5. Description of the invention (3〇) Microscope image transmission system . Here, FIG. 17 is a schematic diagram showing the process of detecting the position of the specimen image based on the excellent information in this embodiment, and FIG. 18 is a functional block diagram of the microscope image transmission system related to this embodiment. . Figures 9 and 20 are flowcharts for explaining the operation of the microscope image transmission system related to this embodiment. As shown in Fig. 18, the microscope image transmission system can be largely divided into an image pickup section 500, an internal processing section 501, a user operator 502, and a microscope 503. A video camera unit 20 j and a video rate capturing unit 202 are set in the image acquisition unit 500. The microscope 503 is provided with an auto-focusing section 203, a magnification switching section 204, and a stage moving section 205. In the above-mentioned internal processing unit 5 01 ′, there is an image display memory 206 equipped with a memory to display images, a video capture controller 207 to control the video capture unit 202, a specimen image position recognition processing unit 214, and a memory 300. , The external memory memory control unit 214 to control the external memory 218 ′, to control the focus control unit 215 of the automatic focusing unit 203, to control the magnification control unit 216 of the magnification switching unit 204, and to control the load A stage control unit 217 of the stage moving unit 205. In addition, the memory 300 includes each of the brightness memories 209 to 211, the RG brightness difference memory 212, the BG brightness difference memory 213, the RG brightness difference maximum memory 225, the BG brightness difference maximum memory 226, RG threshold memory 227 'BG threshold memory; various fields of! 28. In the above-mentioned user operation section 502, it is set to display and display S due to family gt (" cN-S > Al specification (210x297 male S)! ^ ~~ " ---------- --- install -------- order --------- line (please read the precautions on -f first and then fill out this page) printed by the Employees' Cooperative of Intellectual Property Bureau of the Ministry of Economic Affairs 449662 Α7 __B7 V. Description of the invention (η) The image display section 219 of the image in the image display memory 206 and the specimen image position identification and designation section 220 * The image storage / call instruction section specifies the focus control focus by the focus control section 215 The designation unit 222, the magnification designation unit 223 designated by the aforementioned magnification control unit 216, and the stage position designation unit 224 designated by the aforementioned stage control unit 217 to control the stage control. The following description of The process of detecting the position of the sample image that constitutes the realization. In this embodiment, 'the sample image under the microscope is taken through the video camera function unit 201, and converted into digital data by the video rate capture unit 202' The digital data is taken into the image display memory vessel 206 as a still The image information is displayed on the image display unit 219. The specimen image is shown in Fig. 17A, and the display example shown in Fig. 17A 圊 is provided on the glass of the microscope where the specimen image is stored outside the area of the specimen image. Covers the area of shadows, dust, etc. The brightness information 'as shown by color separation' at the arbitrary γ-coordinate position of the specimen image of 17A 囷 is shown in Fig. 17B. Background section And noise, the changes in the red and green luminance information are presumed to be the same, and where the specimen exists, that is, the difference between the red and green luminance information. In general, the pathological specimen image is used for Observation and various staining, so the position of the specimen image can be calculated using the brightness difference. In the 17th example, a pathological specimen stained with a red body as a whole is taken as an object, but it is characterized by staining The color information is different β. Therefore, three kinds of information of red and green 'blue are obtained for each luminance information. (Please read the precautions of the threat surface before filling out this page) -I inn 』SJI .1 nn ϋ IIII _ ϋ I [ϋ n I * n
經濟部智慧財產局員工消費合作让印发 Α7 Β7 五、發明說明(32 ) 取上述紅與綠之輝度資訊的差值,而所顯示之樣子就 如在第17C圖所示。依據該圖之特性而予算出紅與綠之輝 度差值的最大值Rgref,並以其為依據而予設定臨限值。 將此該臨限值為低之X座標值的資料作為FFh,而高於臨 限值者作為0之資料的情形,表示在第17D圖。如是之演 算於所有之Y座標均予進行之結果,而所示者為第17E圖 〇 於上述之例,係表示使用紅與綠之輝度差資訊予以檢 出標本像位置之例,惟,由於標本當然亦可依藍與紅之輝 度差資訊,或者,組合紅與綠之輝度差及藍與綠之輝度差 而予檢出標本像位置。 以下,參照第19及20圖之流程圖,說明使用上述輝度 差資訊欲予辨識標本像位置之動作。 首先,將標本全體影像之全領域的紅綠藍(以下,稱 為RGB)之輝度資訊,格納在各輝度記憶體2〇9、21〇、211( 步驟S301)。於此,將紅(R)之輝度資訊設為尺如叫乂,γ) 、綠(G)之輝度資訊為Gdata(X,Y)、藍(B)之輝度資訊為 Bdata(X,Y)之2次元的排列而予格納在各輝度記憶體2〇9 至211。而X為〇〜(X畫素數“)之範圍,且丫為〇〜(γ畫素 數-丨)之範圍》 其次,將在同一畫素上之各輝度差資訊,格納在輝度 差δ己憶體2丨2、213(步棘S302)。即,取R與G之輝度資訊 差值(RGref(X ’ γ)),且予格納在RG輝度差記憶體2丨2, 並取B與G之輝度差資訊差值(BGref(x,γ)),且予格納在 夂氓張H遺用中闯西家標準(CNS〉A_J規格(;Μ〇 χ 29Γ公发 35 -------------裝·------—訂 * J I I----- (諳先閲讀背面之注意事項再填寫本頁) 449662 A7 B7 五、發明說明(33 ) BG輝度差記憶體213。 (請先閱讀货面之注意事項再填寫本頁) RGref(X ♦ Y)=Rdata(X - Y)-Gdata(X > Y) RGref(X * Y)=Rdata(X * Y)-Gdata(X - Y) 接著,檢出各輝度差值之最大值(RGrefmax、 BGrefmax),且予格納在RG輝度差最大值記憶體225、BG 輝度差最大值記憶體226(步驟S303)»由該輝度差值之最 大值而將標本像辨識用之臨限值,予以格納在RG臨限值 記憶體227、BG臨限值記憶體228(步驟S304) » 於此,參照第20圖之流程圖,予以詳細說明上述臨限 值之設定的順序。臨限值之設定,係從RG輝度差最大值 記憶體225、BG輝度差最大值記憶體226,予以調出各輝 度差值之最大值(RGrefmax、BGrefmax)(步驟S401),且予 選擇標本染色種別(步驟S402)。然後,因應該標本染色種 別進行臨限值設定(RGrefth、BGrefth)(步驟S403)。 RGrefth= RGrefmax/n BGrefth= RGrefmax/η 經濟部智慧財產局員工湞費合作社印製 於此,η,m之值乃因標本染色種別而變。又,能使 其任意變更當然亦可以。例如,設n==m=2,而將R與G 之輝度差的最大值(RGrefmax)之半值,及B與G之輝度差 的最大值(BGrefmax)之半值,作為臨限值RGrefth、BGrefth 亦可以。 又’依染色法亦有藍主體之標本〇將此場合之η,m 之值設為η = RGrefman,m=2。n=l之場合,即,成為 RGrefth= RGrefmax,乃將由R與G之輝度差的資訊之臨限 本纸張尺度適用中國國家標準(CNS)A4規格(210 X 297公藶) 36Consumers ’cooperation with the Intellectual Property Bureau of the Ministry of Economic Affairs for printing and distribution Α7 Β7 V. Invention Description (32) Take the difference between the above red and green luminance information, and the displayed form is as shown in Figure 17C. The maximum value Rgref of the luminance difference between red and green is calculated based on the characteristics of the figure, and the threshold is set based on it. The case where the data with the threshold value of the lower X-coordinate value is taken as FFh, and the data with the value above the threshold is 0 is shown in Fig. 17D. If it is the result of calculation at all Y coordinates, and the one shown is the figure 17E. In the above example, it is an example that uses the information of the difference between red and green to detect the image position of the specimen. However, because Of course, the specimen can also detect the position of the specimen image based on the blue and red luminance difference information, or by combining the red and green luminance differences and the blue and green luminance differences. Hereinafter, referring to the flowcharts of FIGS. 19 and 20, the operation for identifying the position of the specimen image using the above-mentioned luminance difference information will be described. First, the red, green and blue (hereinafter, referred to as RGB) luminance information of the entire image of the specimen is stored in each luminance memory 209, 21, and 211 (step S301). Here, the luminance information of red (R) is set as a rule, such as 乂, γ), the luminance information of green (G) is Gdata (X, Y), and the luminance information of blue (B) is Bdata (X, Y). The 2-dimensional permutation is given to Gurner in each luminance memory 209 to 211. X is in the range of 0 ~ (X pixel number "), and ya is in the range of 0 ~ (γ pixel number- 丨)" Second, the brightness difference information on the same pixel will be equal to the difference in luminance δ Membrane 2 丨 2, 213 (step spine S302). That is, take the difference between the luminance information of R and G (RGref (X 'γ)), and give it to the RG luminance difference memory 2 丨 2, and take B The difference in luminance information between G and G (BGref (x, γ)), and Yugner broke the Western standard (CNS> A_J specification (CNS> A_J specifications) in the widow Zhang H's legacy; Μχχ 29Γ public issue 35 ---- --------- Installation ---------- Order * JI I ----- (谙 Read the precautions on the back before filling in this page) 449662 A7 B7 V. Description of the invention (33) BG brightness difference memory 213. (Please read the precautions on the front of the product before filling in this page) RGref (X ♦ Y) = Rdata (X-Y) -Gdata (X > Y) RGref (X * Y) = Rdata (X * Y) -Gdata (X-Y) Next, the maximum value of each luminance difference (RGrefmax, BGrefmax) is detected, and the Gurner is stored in the RG luminance difference maximum memory 225 and the BG luminance difference maximum memory 226. (Step S303) »The threshold value for identifying the specimen image is determined by the maximum value of the luminance difference The RG threshold memory 227 and the BG threshold memory 228 (step S304) »Here, referring to the flowchart of FIG. 20, the order of setting the above thresholds will be described in detail. The maximum value of each luminance difference (RGrefmax, BGrefmax) is retrieved from the RG luminance difference maximum memory 225 and the BG luminance difference maximum memory 226 (step S401), and the specimen staining type is selected (step S402) Then, set the threshold value (RGrefth, BGrefth) according to the type of specimen staining (step S403). RGrefth = RGrefmax / n BGrefth = RGrefmax / η This is printed here by the employee ’s cooperative of the Intellectual Property Bureau of the Ministry of Economy, η, m The value varies depending on the type of specimen staining. Of course, it can be changed arbitrarily. For example, let n == m = 2 and set the half of the maximum value (RGrefmax) of the luminance difference between R and G, and The half of the maximum value of the difference in luminance between B and G (BGrefmax) can also be used as the thresholds RGrefth and BGrefth. Also, according to the dyeing method, there are also specimens with blue subjects. In this case, the values of η and m are set to η = RGrefman, m = 2. When n = 1, that is, RGrefth = RGr efmax is the threshold of the information based on the difference in brightness between R and G. The paper size applies the Chinese National Standard (CNS) A4 (210 X 297 cm). 36
經濟部智慧时產局3'工消費合泎'ti.5VVY A7 B7 五、發明說明(34) 值設定提高至最大準位。由於如此,即,等於僅以B與G 之輝度差的臨限值設定予以檢出標本像位置a 再度回到第19圖之說明。如上述,決定臨限值(RGrefth 、BGrefth)之後,就予設定欲予辨識檢測標本像之領域( 步驟S305)»欲予辨識標本全體像之位置的場合,乃為Xmin =0 ’ Xmax = (X畫素數-1)、Ymin=0、ΥΓη3χ=(Υ畫素數-1) c 又’由於決定領域而從畫像整體欲予檢測一部份之領 域亦可能。決定欲予檢測之領域之時,就予進行判斷於該 領域内是否存在標本像。 首先’設定欲作標本像辨識檢測之初期值(X = Xtnin ’ Ymin)(步驟 S306)。 以如是’設定初期值之後,進行比較各輝度差資料 (RGref、BGref)與標本像辨識用臨限值(RGref、BGref), 且予進行檢測是否存在標本像。首先,進行比較在任意之 X ’ Y座標上述的紅綠輝度差資料(RGref(X,Y)),與紅綠 輝度差臨限值(RGrefth)(步驟S307)。 於此’若 RGref(X,Y) > RGrefth,即,在(X,γ)座標 上有存在標本像(步驟S3 10)。 並且’若RGref(X,Y) S RGrefth,即,進行因藍綠輝 度差之標本像辨識檢測(步驟S308)。於此,RGref(X,Y) > RGrefth,即’在(X,γ)座標上有存在標本像(步騍S3 1〇) '相對地,若 RGref(X,Y)SBGrefth,即,在(X,γ)座標 上不存在標本像(步驟S309) °如是,在任意之(X,γ)座標 I -I I n I . H ί ϋ ί 一 5, » ϋ ϋ ϋ n 1« t (請先閲讀背面之注意事項再填寫本頁) 37 449662 A7 B7 五、發明說明(35 ) 上的標本像存在檢測乃予終了,因此,將進行其次之座標 的標本像檢測》 即’將又座標增量(X=X+ 1)(步驟S311),並予檢測 新X座標值是否超過最大值(Xmax)(步驟S312)。若X座標 超過最大值(Xmax) ’即恢復至檢測在該座標上之標本像 存在的處理(步驟S307)。若Y座標超過最大值(Ymax)之時 ,即’於此已確認所有的標本像辨識檢測領域而將終了。 《第9實施態樣》 其次,說明本發明之第9實施態樣。該第9實施態樣, 係將網目擴大界限指定以自動予以指定之後,於上述網目 擴大指定界限之中,以自動予以檢出於最初作自動調焦指 定之網目擴大界限位置者。 首先,第21圖係表示在標本整體像以自動進行網目擴 大界限指定之後的樣子。在該第21圖,將個別之網目擴大 指定界限的寬度設為Frame Width,而高度為Frame Height ,且將個別之網目擴大指定界限的中心座標為(X〇c,Y〇c) 〜(X3c,Y3c) >座標之頂點為該圖之左上。 以下,參照第22圖之流程圖詳細說明在標本整體像, 以自動進行網目擴大指定界限之後,將於最初執行自動調 焦之處所以自動予以設定之順序。 首先,檢測網目擴大指定界限之個數(Mesh Num)(步 驟S601)。接著,為持有網目擴大指定界限個別之標本像 辨識畫素數資料,乃將標本像存在檢測用計數器設定為排 列資料(ChkCounter(O)〜ChkCounter(Mesh Num-1))(步驟 本紙張&度適用中國國家標準(CNS)A4規格(210 x 297公釐) -38 - (諳先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 》eJ n I n n I I I n I n n n n 1 n n n It n n It n I I— . 經濟部智慧时4局ar Η消费合泎iifr7,'« A7 B7 五、發明說明(36 ) S602)。又,作網目擴大指定界限個別之標本像存在檢測 ,而將作為標本像所辨識之畫素數資料加入於 (ChkCounter(O)〜ChkCounter(Mesh Num-1))(步驟 S603)。 於此,參照第23圖之流程圖,予以說明有標本像存在 之畫素數資料之檢測的詳細順序。 首先,設定為辨識標本像之用的輝度差臨限值 (BGrefth、BGrefth),且予格納在依賴側端末個人電腦内 之耒圖示的記憶體(步騍S701)。對於臨限值設定乃如前述。 然後,予以設定欲予判斷是否存在標本像之座標的初 期值(步驟S702)。於此,Xic與Yic乃相當於第21圖之(X〇c ,YOc)〜(X3c,Y3c) ° Frame Width 與 Frame Height 乃是網 目擴大指定界限之寬度與高度。 接著’決定座標之初期值之後,就予消除為判斷是否 存在標本像之用的檢測用計數器(步驟S703)。 其次’予以變化XY座標而取得在個別晝素上之輝度 差資訊(RGref(X,Y)、BGref(X ’ Y)),且進行比較其輝度 差資訊與上述步驟S701所決定之輝度差臨限值(RGrefth、 BGrefth)(步驟S704)。於此’若比輝度差臨限值(RGrefth 、BGrefth)為大時’就將檢出測用計數器予以增量(步驟 S705)。而後,將X座標增量而予準備其次之座標(步驟S7〇6) 。另方面,若X座標在於網目擴大指定界限内之領域時( 步驟S707) ’即’將Y座標增量且將X座標恢復為初期值( 步驟S 7 0 8)。而後’檢測Y座標是否為網目擴大指定界限 内之頜域外(步驟S709)。而且,若於領域内,就恢復為上述 通甲由國S家悻準(C'NSm4硯格(2.U) X 297公釐 -------------裝--------訂---------線 {請先閱讀背面之注意事項再填寫本頁) 39 49 6 6 fc Α7 經濟部智慧財產局員工消費合作社印Μ 〜------ B7____五、發明說明(37) 步驟S704。 如是’在個別之網目擴大指定界限上有標本像存在之 時’就與計數所辨識之畫素數》若γ座標位於網目擴大指 定界限之領域範圍内的領域外之時(步驟S7〇9),即,在網 目擴大指定界限内所有之Y座標上,已取得辨識為標本像 之畫素數•而作為標本像所辨識之畫素數就作為資料加入 在 ChkCounter之令(步驟 S710)。 再回到第22圈之說明。將作為標本像所辨識之畫素數 記憶在ChkCounter( 1)。於此,(I)係表示任意之網目擴 大指定界限號碼(相當於第21圖之〇〜3)。當所有之網目擴 大指定界限的標本像存在檢出測終了之時,就從標本像存 在檢測用計數器之值較多者依序予以排列。該所排列之順 序乃作為另一變數而予記憶(步期iS604) » 其次,從標本像存在檢測用計數器值較多者,依序予以判 斷在中心附近是否存在標本(步驊S605)。若判斷為在中心 附近不存在標本像之場合(步驟S606),即,再度回到上述 步驟S605,而再取出標本像存在檢測用計數器之值較多 的網目擴大指定界限,且予判斷在中心附近是否存在標本 像。 於此,在中心附近有標本像存在之時,就作為於最初 為執行AF之位置而予記憶(步驟S607)。 如上述,從多數之網目擴大指定界限之中,能以自動 予以辨識具有最能作為標本像之畫素數,且將在中心附近 有存在標本像之▲所於最初作AF之位置。 本紙張尺度適用中國因家標準(CNS)A4规格<210x297公* ) - 40 <靖先閱讀背面之注意事項再填寫本頁)Wisdom and Industry Bureau of the Ministry of Economic Affairs 3'Working and Consumption Combination 'ti.5VVY A7 B7 V. Description of the invention (34) The value setting is increased to the maximum level. Because of this, it is equal to detecting the specimen image position a only with the threshold setting of the difference in luminance between B and G, and it returns to the description of FIG. 19 again. As described above, after the threshold values (RGrefth, BGrefth) are determined, the area where the specimen image is to be identified is determined (step S305) »Where the position of the entire image of the specimen is to be identified, it is Xmin = 0 'Xmax = ( X painting prime number -1), Ymin = 0, ΥΓη3χ = (Υpainting prime number -1) c It is also possible to detect a part of the whole image from the whole picture because it determines the field. When the area to be tested is determined, it is judged whether a specimen image exists in the area. First, an initial value (X = Xtnin 'Ymin) of the specimen image recognition detection to be performed is set (step S306). After setting the initial value as described above, each luminance difference data (RGref, BGref) is compared with a threshold value (RGref, BGref) for specimen image recognition, and the presence of a specimen image is detected. First, the red-green luminance difference data (RGref (X, Y)) described above at an arbitrary X'Y coordinate is compared with the red-green luminance difference threshold value (RGrefth) (step S307). Here, if RGref (X, Y) > RGrefth, that is, there is a specimen image on the (X, γ) coordinate (step S3 10). And 'if RGref (X, Y) S RGrefth, that is, the specimen image recognition detection is performed due to the blue-green luminance difference (step S308). Here, RGref (X, Y) > RGrefth, that is, 'there is a specimen image present at (X, γ) coordinates (step S3 1〇)' Conversely, if RGref (X, Y) SBGrefth, that is, in There is no specimen image on (X, γ) coordinates (step S309) ° If so, at any (X, γ) coordinates I -II n I. H ί ϋ ί a 5, »ϋ ϋ ϋ n 1« t (Please (Read the precautions on the back before filling this page) 37 449662 A7 B7 V. The specimen image detection on the description of invention (35) is over. Therefore, the specimen image detection of the second coordinate will be performed. (X = X + 1) (step S311), and it is detected whether the new X-coordinate value exceeds the maximum value (Xmax) (step S312). If the X coordinate exceeds the maximum value (Xmax) ', it returns to the process of detecting the existence of the specimen image on the coordinate (step S307). If the Y-coordinate exceeds the maximum value (Ymax), that is to say, “All the specimen images have been identified and detected, and it will end. "Ninth Embodiment" Next, a ninth embodiment of the present invention will be described. In the ninth embodiment, after the mesh expansion limit designation is automatically designated, among the above-mentioned mesh expansion designation limits, a person who automatically detects the position of the mesh expansion limit that was originally designated for auto-focusing is automatically detected. First, Fig. 21 shows how the entire specimen image is automatically designated by the mesh expansion limit. In FIG. 21, the width of the individual mesh expansion designation limit is set to Frame Width, the height is Frame Height, and the center coordinates of the individual mesh expansion designation limit are (X〇c, Y〇c) to (X3c , Y3c) > The vertex of the coordinates is the upper left of the figure. Hereinafter, referring to the flowchart of FIG. 22, the sequence of setting the automatic focus will be performed first after the entire image of the specimen is automatically expanded to specify the limit. First, the number of mesh expansion designated meshes (Mesh Num) is detected (step S601). Next, in order to hold the individual image data of the specimen image for specifying the limit of the mesh expansion, the specimen image presence detection counter is set as the arrangement data (ChkCounter (O) ~ ChkCounter (Mesh Num-1)) (Step Paper & Degree applies to China National Standard (CNS) A4 specification (210 x 297 mm) -38-(谙 Please read the notes on the back before filling this page) Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Consumption Cooperatives eJ n I nn III n I nnnn 1 nnn It nn It n II—. 4 bureaus of the Ministry of Economic Affairs ar Ηconsumption combination iifr7, '«A7 B7 V. Description of the invention (36) S602). In addition, the presence of individual specimen images for which the mesh is enlarged and specified is detected, and the pixel number data identified as the specimen images are added to (ChkCounter (O) ~ ChkCounter (Mesh Num-1)) (step S603). Here, with reference to the flowchart of FIG. 23, a detailed procedure for detecting the pixel number data in which a specimen image exists will be described. First, set the thresholds (BGrefth, BGrefth) for recognizing the specimen image, and store the icon memory in the personal computer at the end of the reliance (step S701). The threshold setting is as described above. Then, an initial value is set to judge whether or not the coordinates of the specimen image exist (step S702). Here, Xic and Yic are equivalent to (X〇c, YOc) ~ (X3c, Y3c) ° Figure 21 Frame Width and Frame Height are the width and height of the designated boundaries of the mesh expansion. Next, after determining the initial value of the coordinates, a detection counter for judging whether or not a specimen image exists is eliminated (step S703). Secondly, change the XY coordinates to obtain the luminance difference information (RGref (X, Y), BGref (X'Y)) on individual celestial elements, and compare the luminance difference information with the luminance difference determined by step S701 above. Limit values (RGrefth, BGrefth) (step S704). Here, "if the luminance difference thresholds (RGrefth, BGrefth) are larger", the detection counter is incremented (step S705). Then, the X coordinate is incremented to prepare a next coordinate (step S706). On the other hand, if the X coordinate is in the area within the designated range of the mesh expansion (step S707), that is, the Y coordinate is incremented and the X coordinate is restored to the initial value (step S708). Then, it is detected whether or not the Y coordinate is outside the jaw area within the designated limit of mesh expansion (step S709). Moreover, if it is in the field, it will be restored to the above-mentioned Tongjia by the State S family (C'NSm4 砚 格 (2.U) X 297 mm ------------ install- ------ Order --------- line {Please read the notes on the back before filling out this page) 39 49 6 6 fc Α7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs ~~- --- B7____ V. Description of the invention (37) Step S704. If it is "when specimen images exist on the individual mesh expansion designation limit", then the number of identified pixels is counted. "If the γ coordinate is outside the area within the range of the mesh expansion designation limit (step S709) That is, on all the Y coordinates within the designated range of the mesh expansion, the number of pixels identified as the specimen image has been obtained, and the number of pixels identified as the specimen image is added to ChkCounter's order as data (step S710). Return to the explanation on lap 22. The number of pixels identified as the specimen image is stored in ChkCounter (1). Here, (I) indicates an arbitrary mesh expansion designation limit number (equivalent to 0 to 3 in Fig. 21). When all the specimen images whose meshes expand the designated limit are detected, the specimen images are sorted in order from the specimen image that has more counters for detection. The sequence of this arrangement is memorized as another variable (step iS604) »Second, from the specimen image presence detection counter with a large value, it is judged in order whether a specimen exists near the center (step S605). If it is determined that the specimen image does not exist near the center (step S606), that is, return to the above step S605 again, and then take out a mesh with a large number of specimen image presence detection counters and expand the designated limit, and judge that it is at the center. Is there a specimen image nearby. Here, when a specimen image exists near the center, it is memorized as the position where AF was originally performed (step S607). As mentioned above, from the expanded designation limit of most meshes, the number of pixels that can best be used as the specimen image can be automatically identified, and there will be a spot where the specimen image exists near the center of the original AF position. This paper size applies to China Standards (CNS) A4 specifications < 210x297 male *)-40 < Jing first read the precautions on the back before filling this page)
-n n I w--------訂 -線! >i ——- -------------- 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明說明(38 ) 以上’對於本發明之實施態樣作說明,惟,本發明並 不限定於此,而當然不脫離其主旨之範©,可作種種之改 良或變更》例如,於上述實施態樣,係將網目分割之起點 作為靜止影像之左上而終點為右下,惟,當然,亦能將靜 止影像中之任意的位置作為起點、終點。 如上述詳述,依據本發明,即在顯微鏡影像之遠距觀 察上’將於初期觀察影像上之觀察領域的網目分割有關之 指定,依簡單之操作而能予提供有效率且能正確進行之顯 微鏡影像傳送系統、顯微鏡影像處理方法及使用於顯微鏡 影像處理之記錄媒體。 圊式之簡單說明 第1圈係表示本發明之顯微鏡影像傳送系統的構成之 概略圊* 第2圖係表示將第1圊之構成的一部份具體化之方塊圓 〇 第3圖係表示本發明之顯微鏡影像傳送系統的動作之 流程囷。 第4(A)至(D)圓係由第1實施態樣有關之顯微鏡影像傳 送系統作標本像位置之影像辨識上的一連串處理之說明囷 〇 第5圖係說明由對於第1實施態樣有關之顯微鏡影像傳 送系統的網目資料製作之過程的流程圖β 第6圖(Α)至(Ε)係說明由與第1實施態樣有關之顯微鏡 影像傳送系統作成網目資料之過程。 本紙張尺度適用中因S家搮準(CNS>A4規格(210 X 297公釐) 41 ------------裳 - ------ 訂 --------線 (請先閱讀背面之注意事項再填寫本頁) 449662 A7 Β7 五、發明說明(39 ) 第7園係有關與第2實施態樣之顯微鏡影像傳送系統作 成網目資料之過程的說明圖。 第8圖係由與第3實施態樣有關之顯微鏡影像傳送系統 作成網目資料之過程的說明圖。 第9圖係說明由與第3實施態樣有關之顯微鏡影像傳送 系統作成網目資料之過程的流程圖》 第10圖係說明於第9圖所使用之區段内的標本像所存 在之面積比為50%以下之場合,其區段内再以更高倍指定 處理(以下,稱為區段内之網目指定)之流程圖。 第11圖係予說明由第5實施態樣有關之顯微鏡影像傳 送系統作網目界限之移動操作用之流程圖。 第12圖係由第5實施態樣有關之顯微鏡影像傳送系統 作網目界限之移動操作的說明圖》 第13圖係說明由第6實施態樣有Μ之顯微鏡影像傳送 系統作自動調焦位置辨識動作之用的流程圖。 第14圖係說明由第6實施態樣有關之顯微鏡影像傳送 系統作自動調焦位置辨識動作之用。 第15圖係表示產生周邊光量不足之宏觀像的一侧。 經濟部智慧財產局員Η消費合作社印发 第16圖係詳細說明對應於周邊光量不足而由第7實 施態樣有關之顯微鏡影像傳送系統作網目分割指定之動作 的流程圖* 第17(A)至(Ε)圖係表示於第8實施態樣,由於色資訊 抽出而予檢出標本像位置之過程的模式圖。 第18圖係第8實施態樣有關之顯微鏡影像傳送系統 42 ί請先閱讀臂面之注$項再填寫本頁> 本紙張又度適用t國國家標準(CNS)A4規格(210 X 297公釐) A7 ---—一 B7_______ 五、發明說明(4〇 ) 的機能方塊囷。 第1 9圖係為說明第8實施態樣有關之顯微鏡影像傳 送系統的動作之用的流程圖。 第20圖係為說明第8實施態樣有關之顯微鏡影像傳 送系統的動作之用的流程圖。 第2丨圖係表示欲詳細說明由第9實施態樣有關之顯 微鏡影像料系統,以自動對標本整时進行網目擴大指 定界限之後,將於最初予以執行自動調焦之處所以自動予 以設定之順序的流程圖。 第22圖係予詳細說明由第9實施態樣有關之顯微鏡 影像傳送系統,以自動對標本整體像進行網目擴大指定範 圍之後’將於最初予以執行自動調焦之處所以自動予以設 定之順序的流程圖。 第23圖係予說明由第9實施態樣有關之顯微鏡影像 傳送系統作標本像所存在之畫素數資料的檢測之詳細順序 的流程圖。 I--H — — — — — — ^ il — ί— ------I (請先閱讀背面之注意事項再填寫本頁) 經濟耶智¾时產局員工消f合汴钍4¾ 一105-¥ I規 4 A S) -C *'f; If. •ί.^ -¾ a, '"---ί 43 449662 A7 B7 五、發明說明(41 元件標號對照 經濟部智慧財產局員工消費合作社印製 表紙張尺度適用中國國家標準(cns)A4 x 297 51 52 53 55 1…觀察側個人電腦 2,4···監控器 3…公用線路(ISDN) 5…依賴側個人電腦 6…視頻攝影機 7 ’ 503…顯微鏡 8…電動旋轉器 9…電動載物台 12…顯微鏡控制台 13,501…内部處理部 14…使用者介面部 21…攝影部 22 ’ 204…倍率切換部 23,205…載物台移動部 24,203…自動調焦部 25…自動調整光圈部 26…畫面記憶部 27,216“·倍率控制部 28…載物台控制部 29,2 15…焦點控制部 30…可變光圈控制部 31…外部記憶部' 32…標本領域辨識部 33…區段分割部 34…相關關係資訊記憶部 35…顯示部 36,223…倍率指定部 3 7…區、肖餘、移鱗定部 背景部份 標本像 雜音部份 標本像上限輝度準位 56…標本像下限輝度準位 63…區段 64〜67,71〜76…網目位置 201…視頻攝影機部 2〇1…視頻捕捉部 206···影像顯示記憶體 2〇7_"視頻捕捉控制部 2 0 8…標本像位置辨減理部 209〜211…各輝度記憶體 212…RG輝度記憶體 213…BG輝度記憶體 214…標本像位置辨識控制部 44 (請先閱璜嘴面之注意事項再填寫本頁) rk - - -----I ---I-----. Ί---I I I — I______________ A7 B7 五、發明說明U2 ) 217··*載物台控制部 2.1 8···外部記憶記憶體 219…影像顯示部 2 2 0…標本像位置辨識指定部 22卜影像保存/讀出指定部 222…焦點指定部 224…載物台位置指定部 2 2 5…RG輝度差最大值記憶體 2 2 6…BG輝度差最大值記憶體 227".RG臨限值記憶體 228— BG臨限值記憶體 300…記憶體 500…影像攝入部 502…使用者操作部 --------—---装 * — (請先閱讀背面之注意事項再填寫本頁> 訂---------線 經濟部智慧財產局員工消費合ίΐ·ί1κ3ί:ϊ1 45 --n n I w -------- order -line! > i ——- -------------- Printed by the Consumers 'Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Description of Invention (38) Above' Describe the implementation of the present invention However, the present invention is not limited to this, and certainly does not deviate from the spirit of its subject. Various improvements or changes can be made. For example, in the above embodiment, the starting point of the mesh segmentation is the upper left of the still image and the ending It is the bottom right, but, of course, any position in the still image can be used as the starting point and ending point. As detailed above, according to the present invention, in the long-distance observation of the microscope image, the designation related to the mesh division of the observation field in the initial observation image can be provided efficiently and correctly by simple operations. Microscope image transmission system, microscope image processing method, and recording medium used for microscope image processing. Brief description of the formula: The first circle shows the outline of the structure of the microscope image transmission system of the present invention. * The second circle shows a square circle which embodies a part of the structure of the first circle. The third circle shows this The operation flow of the invention's microscope image transmission system. The fourth (A) to (D) circle is a series of processes for image recognition of the specimen image position by the microscope image transmission system related to the first embodiment. Figure 5 illustrates the first embodiment The flowchart of the process of making the mesh data of the relevant microscope image transmission system β Figures 6 (A) to (E) illustrate the process of making mesh data by the microscope image transmission system related to the first embodiment. The size of this paper is suitable for the standard of S house (CNS > A4 size (210 X 297 mm) 41 ------------ Shang ------- Order -------- --- line (please read the precautions on the back before filling this page) 449662 A7 Β7 V. Description of the invention (39) The explanation diagram of the 7th department about the process of making mesh data with the microscope image transmission system of the second embodiment Fig. 8 is an explanatory diagram of the process of generating mesh data by the microscope image transmission system related to the third embodiment. Fig. 9 is an explanation of the process of generating mesh data by the microscope image transmission system related to the third embodiment. The flow chart in Figure 10 illustrates the case where the area ratio of the specimen image in the section used in Figure 9 is less than 50%, and the section is designated at a higher magnification (hereinafter, referred to as the section). The flow chart of the mesh designation in the paragraph. Figure 11 is a flow chart illustrating the operation of moving the mesh limit by the microscope image transmission system related to the fifth embodiment. Figure 12 is related to the fifth embodiment. Schematic diagram of the operation of moving the mesh boundary of the microscope image transmission system》 Part 1 Fig. 3 is a flowchart illustrating the automatic focusing position identification operation performed by a microscope image transmission system having an M in the sixth embodiment. Fig. 14 illustrates automatic adjustment by a microscope image transmission system in the sixth embodiment. Figure 15 is used to identify the focus position. Figure 15 shows the side where a macro image with insufficient ambient light is generated. Printed by a member of the Intellectual Property Bureau of the Ministry of Economic Affairs and Consumer Cooperatives. Figure 16 is a detailed description of the seventh mode of implementation corresponding to insufficient ambient light. Flow chart of the operation of the related microscope image transmission system for the designation of mesh segmentation * Figures 17 (A) to (E) show the mode of the eighth embodiment, the process of detecting the image position of the specimen due to the extraction of color information Figure 18. Figure 18 is a microscope image transmission system related to the eighth embodiment 42 Please read the note on the arm surface before filling in this page> This paper is again applicable to the national standard (CNS) A4 specification (210 X 297 mm) A7 ----- B7_______ V. Functional block of invention description (40) 4 Figure 19 is a flowchart for explaining the operation of the microscope image transmission system related to the eighth embodiment. Fig. 20 is a flowchart for explaining the operation of the microscope image transmission system related to the eighth embodiment. Fig. 2 丨 shows the detailed description of the microscope image material system related to the ninth embodiment to automatically After the specimen has been expanded with the specified mesh limit all the time, the flow chart of the order of automatic setting will be performed at the beginning of the automatic focusing. Figure 22 is a detailed explanation of the microscope image transmission system related to the ninth embodiment. A flowchart of the order in which the auto-focusing will be performed initially after the mesh enlargement of the entire specimen image is automatically specified. Fig. 23 is a flowchart illustrating a detailed procedure for detecting the pixel number data existing in the specimen image by the microscope image transmission system related to the ninth embodiment. I--H — — — — — — — ^ il — ί — ------ I (Please read the precautions on the back before filling out this page) 105- ¥ I Regulation 4 AS) -C * 'f; If. • ί. ^ -¾ a,' " --- ί 43 449662 A7 B7 V. Description of the invention (41 component numbers are compared with employees of the Intellectual Property Bureau of the Ministry of Economic Affairs The paper size of the printed sheet of the consumer cooperative is applicable to the Chinese national standard (cns) A4 x 297 51 52 53 55 1 ... the personal computer on the observation side 2, 4 ... monitor 3 ... the personal computer 6 on the dependent side 6 ... Video camera 7 '503 ... microscope 8 ... motorized rotator 9 ... motorized stage 12 ... microscope console 13,501 ... internal processing unit 14 ... user interface 21 ... photographing unit 22'204 ... magnification switching units 23,205 ... the stage moving section 24,203 ... the auto focus section 25 ... the automatic adjustment of the aperture section 26 ... the screen memory section 27,216 "· magnification control section 28 ... the stage control section 29, 2 15 ... the focus control section 30 ... Variable aperture control section 31 ... external memory section 32; specimen area identification section 33 ... section division section 34 ... relational information memory Part 35 ... Display part 36,223 ... Magnification designation part 3 7 ... Zone, Xiao Yu, Scale shifting background part specimen like noise part specimen like upper limit brightness level 56 ... specimen lower limit brightness level 63 ... section 64 to 67, 71 to 76 ... Mesh position 201 ... Video camera unit 201 ... Video capture unit 206 ... Video display memory 2 07_ " Video capture control unit 2 0 8 ... Specimen image position discrimination subtraction unit 209 ~ 211 ... intensity memory 212 ... RG intensity memory 213 ... BG intensity memory 214 ... specimen position recognition control unit 44 (please read the precautions of the mouth surface before filling this page) rk------ -I --- I -----. Ί --- III — I______________ A7 B7 V. Description of the invention U2) 217 ·· * Stage control unit 2.1 8 ··· External memory 219 ... Image display unit 2 2 0… specimen position recognition designation unit 22 image storage / readout designation unit 222… focus designation unit 224… stage position designation unit 2 2 5… RG brightness difference maximum value memory 2 2 6… BG brightness difference Maximum memory 227 " .RG threshold memory 228—BG threshold memory 300 ... memory 500 ... images Entry section 502 ... User operation section ------------- install * — (Please read the precautions on the back before filling this page> Order --------- Wire Economy Department Wisdom Property Consumption of Employees of the Property Bureau ίΐ · ί1κ3ί: ϊ1 45-
Claims (1)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2735799 | 1999-02-04 |
Publications (1)
Publication Number | Publication Date |
---|---|
TW449662B true TW449662B (en) | 2001-08-11 |
Family
ID=12218808
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW089101991A TW449662B (en) | 1999-02-04 | 2000-02-03 | Microscope image transmitting system, method of processing microscope images, and recording medium for use in processing microscope images |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR20000057920A (en) |
CN (1) | CN1175298C (en) |
TW (1) | TW449662B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100454078C (en) * | 2006-06-22 | 2009-01-21 | 北京普利生仪器有限公司 | Method for preparing microscopic image of holographic digitalized sliced sheet |
WO2008052404A1 (en) * | 2006-10-10 | 2008-05-08 | Beijing Huaqi Information Digital Technology Co., Ltd. | A method for using a data processing terminal to control a digital microscope to output image |
CN102305791B (en) * | 2011-07-19 | 2012-11-07 | 南昌百特生物高新技术股份有限公司 | Slide specimen image acquiring method |
CN105339970A (en) * | 2013-02-06 | 2016-02-17 | 电子商品交易合伙人有限公司 | Methods for recommending jewelry items |
US11222400B2 (en) * | 2016-11-24 | 2022-01-11 | Nikon Corporation | Image processing device, microscope system, image processing method, and computer program for displaying magnified images from different observation directions |
-
2000
- 2000-02-03 TW TW089101991A patent/TW449662B/en active
- 2000-02-03 KR KR1020000005423A patent/KR20000057920A/en not_active Application Discontinuation
- 2000-02-04 CN CNB001019961A patent/CN1175298C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN1175298C (en) | 2004-11-10 |
KR20000057920A (en) | 2000-09-25 |
CN1263273A (en) | 2000-08-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101909156B (en) | Electronic camera and image processing method thereof | |
JP4600395B2 (en) | Microscope system and image processing method | |
TWI289807B (en) | Digital still camera, image reproducing apparatus, face image display apparatus and methods of controlling same | |
JP4462959B2 (en) | Microscope image photographing system and method | |
US7215467B2 (en) | System and method for controlling microscope | |
USRE42794E1 (en) | Information-inputting device inputting contact point of object on recording surfaces as information | |
JP5301970B2 (en) | Digital camera system for microscope and microscope system | |
JP3824116B2 (en) | Microscope image remote control system | |
JP2007523521A (en) | Automatic focus area display | |
WO2002067039A1 (en) | Image comparing device, image comparing method and progrom having computer run image comparison | |
JP2011015163A (en) | Imaging apparatus | |
JP4878815B2 (en) | Microscope equipment | |
EP0678822B1 (en) | Image pick-up apparatus | |
JP4872396B2 (en) | Image editing apparatus, image editing method, and image editing program | |
JP2000295462A (en) | Transmission system for microscope image | |
TW449662B (en) | Microscope image transmitting system, method of processing microscope images, and recording medium for use in processing microscope images | |
US20060178561A1 (en) | Endoscope apparatus | |
JP2008209306A (en) | Camera | |
JP2006301201A (en) | Optical device and camera system | |
JP2009081530A (en) | Imaging apparatus and imaging method | |
KR101663226B1 (en) | Digital image processing apparatus and digital image processing method | |
JPH07191796A (en) | Sight line input system | |
JP2006253805A (en) | Photographing apparatus and method | |
CN111586328B (en) | Information processing apparatus, information processing method, and recording medium | |
JP2006253806A (en) | Photographing apparatus and method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GD4A | Issue of patent certificate for granted invention patent |