TW364924B - Three new cell lines derived from pupal ovaries of Spodoptera litura - Google Patents

Three new cell lines derived from pupal ovaries of Spodoptera litura

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Publication number
TW364924B
TW364924B TW084113841A TW84113841A TW364924B TW 364924 B TW364924 B TW 364924B TW 084113841 A TW084113841 A TW 084113841A TW 84113841 A TW84113841 A TW 84113841A TW 364924 B TW364924 B TW 364924B
Authority
TW
Taiwan
Prior art keywords
cell lines
pupal
ovaries
spodoptera litura
lines derived
Prior art date
Application number
TW084113841A
Other languages
Chinese (zh)
Inventor
Jeng-Ren Shr
Jung-Shiung Wang
Original Assignee
Nat Science Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Science Council filed Critical Nat Science Council
Priority to TW084113841A priority Critical patent/TW364924B/en
Application granted granted Critical
Publication of TW364924B publication Critical patent/TW364924B/en

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Abstract

Three cell lines derived from pupal ovaries of the tobacco cutworm, Spodoptera litura, were designated SL1A, SL7A, and SL7B. The optimal cultural condition of those cells were cultured with TNM-FH medium supplemented with 10% fetal bovine serum in 28C. Using isozyme analysis, karyotype analysis, and growth curve could characterized those three cell lines differ to other insect cell lines. Three cell lines show its susceptible to SINPV and AcNPV, so they were not only provided to study in vitro culture of SINPV, but also to be used in the production of recombinant protein AcNPV expression system.
TW084113841A 1995-12-26 1995-12-26 Three new cell lines derived from pupal ovaries of Spodoptera litura TW364924B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW084113841A TW364924B (en) 1995-12-26 1995-12-26 Three new cell lines derived from pupal ovaries of Spodoptera litura

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW084113841A TW364924B (en) 1995-12-26 1995-12-26 Three new cell lines derived from pupal ovaries of Spodoptera litura

Publications (1)

Publication Number Publication Date
TW364924B true TW364924B (en) 1999-07-21

Family

ID=57940996

Family Applications (1)

Application Number Title Priority Date Filing Date
TW084113841A TW364924B (en) 1995-12-26 1995-12-26 Three new cell lines derived from pupal ovaries of Spodoptera litura

Country Status (1)

Country Link
TW (1) TW364924B (en)

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