TW202327460A - Non-alcoholic beer-flavored beverage - Google Patents

Non-alcoholic beer-flavored beverage Download PDF

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TW202327460A
TW202327460A TW111132909A TW111132909A TW202327460A TW 202327460 A TW202327460 A TW 202327460A TW 111132909 A TW111132909 A TW 111132909A TW 111132909 A TW111132909 A TW 111132909A TW 202327460 A TW202327460 A TW 202327460A
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mass ppm
beer
fermentation
less
yeast
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岸本智之
加藤悠一
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日商三得利控股股份有限公司
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Alcoholic Beverages (AREA)

Abstract

The present invention provides a non-alcoholic beer-flavored beverage having an acetic acid content of 90 to 320 mass ppm and a pH of 2.8 to 4.2. The present invention is capable of providing a non-alcoholic beer-flavored beverage that has a novel taste with an excellent finish and stimulating sensation.

Description

無酒精啤酒風味飲料Non-alcoholic beer flavored beverages

本發明關於一種無酒精啤酒風味飲料及其製造方法。The invention relates to a non-alcoholic beer-flavored beverage and a manufacturing method thereof.

隨著近年來消費者之嗜好之多樣化,期望具有各種香味特徵之無酒精啤酒風味飲料之開發。例如專利文獻1中揭示一種非發酵啤酒風味飲料,其係作為雖然多酚之總含量較高,但之後殘留之澀味非常少,且後勁較佳之非發酵啤酒風味飲料,且其特徵為多酚之總含量為100ppm以上,含有選自環糊精及索馬甜所成群中1種以上之掩蓋素材。且,有時會對無酒精啤酒風味飲料要求刺激感,作為賦予刺激感之方法,有熟知添加碳酸氣體。 [先前技術文獻] [專利文獻] With the diversification of consumers' preferences in recent years, the development of non-alcoholic beer-flavored beverages having various aroma characteristics is desired. For example, Patent Document 1 discloses a non-fermented beer-flavored beverage, which is a non-fermented beer-flavored beverage that has very little residual astringency and good aftertaste although the total content of polyphenols is high, and is characterized by polyphenols The total content is more than 100ppm, containing at least one masking material selected from the group consisting of cyclodextrin and thaumatin. In addition, non-alcoholic beer-flavored beverages may require stimulation, and adding carbon dioxide gas is known as a method for imparting stimulation. [Prior Art Literature] [Patent Document]

[專利文獻1]日本特開2017-79801號公報[Patent Document 1] Japanese Patent Laid-Open No. 2017-79801

[本發明欲解決之課題][Problems to be Solved by the Invention]

然而,專利文獻1之飲料中,為了賦予後勁,必須添加如環糊精或索馬甜之掩蓋素材,要求一種不需要如此之掩蓋素材之新穎技術。且,為了賦予刺激感,若將碳酸氣壓提高,則會有因碳酸而使腹脹等之問題,因此對於刺激感賦予也希望更改善。However, in order to impart stamina to the beverage of Patent Document 1, it is necessary to add a masking material such as cyclodextrin or thaumatin, and a novel technology that does not require such a masking material is required. In addition, in order to impart a stimulating feeling, if the carbon dioxide gas pressure is increased, there is a problem of abdominal distension due to carbonic acid, so it is desired to further improve the stimulating feeling.

本發明關於提供一種收斂感及刺激感優異之新穎無酒精啤酒風味飲料及其製造方法。於此,「收斂感」意指後味有勁且味道收斂。且,「刺激感」意指口腔內或喉嚨有刺痛與刺激,味道會有立體感且賦予滿足感。 [解決課題之手段] The present invention relates to providing a novel non-alcoholic beer-flavored beverage with excellent astringent and stimulating sensations and a manufacturing method thereof. Here, "astringent feeling" means that the aftertaste is strong and the taste is astringent. In addition, "stimulation" means tingling and irritation in the mouth or throat, and the taste gives a sense of three-dimensionality and satisfaction. [Means to solve the problem]

本發明關於下述[1]~[3]。 [1] 一種無酒精啤酒風味飲料,其中,乙酸之含量為90~320質量ppm,pH值為2.8~4.2。 [2] 一種pH值為2.8~4.2之無酒精啤酒風味飲料之製造方法,其中,包含使乙酸成為90~320質量ppm來含有之步驟。 [3] 一種對pH值為2.8~4.2之無酒精啤酒風味飲料賦予收斂感或刺激感之方法,其中包含使乙酸成為90~320質量ppm來含有之步驟。 [發明效果] The present invention relates to the following [1] to [3]. [1] A non-alcoholic beer-flavored beverage, wherein the content of acetic acid is 90-320 mass ppm and the pH value is 2.8-4.2. [2] A method for producing a non-alcoholic beer-flavored beverage having a pH of 2.8 to 4.2, including the step of containing acetic acid at 90 to 320 ppm by mass. [3] A method for imparting an astringent or stimulating feeling to a non-alcoholic beer-flavored beverage having a pH of 2.8 to 4.2, including a step of containing acetic acid at 90 to 320 mass ppm. [Invention effect]

藉由本發明,能夠提供一種收斂感及刺激感優異之新穎無酒精啤酒風味飲料及其製造方法。According to the present invention, it is possible to provide a novel non-alcoholic beer-flavored beverage excellent in astringency and stimulation and a method for producing the same.

本發明者們針對前述課題進行縝密探討結果發現,藉由在無酒精啤酒風味飲料中含有特定量乙酸,會得到刺激感,以及藉由特定之pH值,對來自乙酸之啤酒風味飲料之香味來說,會抑制負面之酸臭味,且同時具有優異之收斂感。雖然此機制尚未確定,但已知乙酸會馬上在口腔內蒸發,作為酸之氣體,會刺激口腔內或喉嚨等。已知酸味會賦予後味之後勁,並能夠有收斂味,藉由將成為酸味之代用指標之pH值設為酸性,能夠賦予收斂感感。進而,pH值與乙酸濃度同樣地,乙酸之揮發效率也會有變化。由以上可推測,藉由使溶液中含有特定量之乙酸量,以及將溶液設定在特定pH值,能夠抑制乙酸所帶來之厭惡的酸臭味,且同時賦予刺激感或收斂感。The inventors of the present invention have conducted careful studies on the above-mentioned problems and found that by adding a specific amount of acetic acid to non-alcoholic beer-flavored beverages, a stimulating sensation can be obtained, and that the aroma of beer-flavored beverages derived from acetic acid can be enhanced by a specific pH value. It is said that it will suppress the negative sour smell, and at the same time it has an excellent astringent feeling. Although the mechanism has not yet been determined, it is known that acetic acid evaporates immediately in the mouth, and as acid gas, it irritates the mouth or throat. It is known that acidity imparts aftertaste and can have an astringent taste, and by making the pH, which is a proxy index of sourness, acidic, it is possible to impart an astringent feeling. Furthermore, the acetic acid volatilization efficiency also changes in the same manner as the pH value and the acetic acid concentration. It can be inferred from the above that by making the solution contain a specific amount of acetic acid and setting the solution at a specific pH value, the unpleasant sour smell caused by acetic acid can be suppressed, and at the same time, a stimulating or astringent feeling can be given.

本發明之無酒精啤酒風味飲料中含有乙酸。本發明之無酒精啤酒風味飲料中之乙酸含量以賦予刺激感之觀點來看,90質量ppm以上較佳為95質量ppm以上,再較佳為100質量ppm以上,更較佳為105質量ppm以上,更較佳為115質量ppm以上,更較佳為120質量ppm以上,更較佳為125質量ppm以上。且,以抑制酸臭味之觀點來看,320質量ppm以下較佳為310質量ppm以下,再較佳為300質量ppm以下,更較佳為250質量ppm以下,更較佳為200質量ppm以下,更較佳為190質量ppm以下,更較佳為180質量ppm以下,更較佳為170質量ppm以下,更較佳為160質量ppm以下,更較佳為150質量ppm以下,更較佳為140質量ppm以下,更較佳為130質量ppm以下。且,能夠設定成此等之值任意組合之範圍。本發明之無酒精啤酒風味飲料能夠包含乙酸鈉等之乙酸鹽。包含乙酸鹽時,乙酸之含量為乙酸及乙酸鹽之合計量。The non-alcoholic beer-flavored beverage of the present invention contains acetic acid. The content of acetic acid in the non-alcoholic beer-flavored beverage of the present invention is at least 90 mass ppm, preferably at least 95 mass ppm, further preferably at least 100 mass ppm, and still more preferably at least 105 mass ppm, from the viewpoint of imparting a stimulating feeling. , More preferably at least 115 mass ppm, More preferably at least 120 mass ppm, More preferably at least 125 mass ppm. In addition, from the viewpoint of suppressing sour odor, it is preferably not more than 320 mass ppm, preferably not more than 310 mass ppm, more preferably not more than 300 mass ppm, still more preferably not more than 250 mass ppm, still more preferably not more than 200 mass ppm , more preferably 190 mass ppm or less, more preferably 180 mass ppm or less, more preferably 170 mass ppm or less, more preferably 160 mass ppm or less, more preferably 150 mass ppm or less, more preferably 150 mass ppm or less 140 mass ppm or less, more preferably 130 mass ppm or less. And, it is possible to set the range in which these values are combined arbitrarily. The non-alcoholic beer-flavored beverage of the present invention can contain acetate such as sodium acetate. When acetate is included, the content of acetic acid is the total amount of acetic acid and acetate.

本發明之無酒精啤酒風味飲料中,乙酸量能夠藉由製造步驟中之發酵生成,或添加乙酸或含有乙酸之素材來調整,以抑制酸臭味之觀點及賦予膨脹度之觀點來看,藉由發酵食品等之素材,使釀造醋生成,或添加釀造醋來調整較佳。本說明書中,「釀造醋」意指包含由酵母、乙酸菌、乳酸菌等之微生物所生成(釀造)之乙酸。有舉例如日本農林規格(JAS)昭和五十四年六月八日農林水產省告第八百一號(最終改正:平成二十年十月十六日農林水產省告示第千五百六號)中記載之釀造醋、食醋之表示相關之公正競爭規約及施行規則(平成30年5月18日施行)中記載之釀造醋等,雖然穀物醋、果實醋、米醋、米黑醋、蘋果醋、葡萄醋等也相當於釀造醋,但不限定於此。亦可在一部分包含由酵母、乙酸菌、乳酸菌等之微生物所生成(釀造)之乙酸,亦可包含製造步驟中使用乙酸菌或乳酸菌等所生成之乙酸,但一般的啤酒之製造方法(酵母之發酵)所生成之乙酸不包含在本發明之釀造醋中。且,此等釀造醋中混合水或其他食品或素材等也稱作本發明之釀造醋。例如,混合糖類或植物萃取物等之食品素材,或核酸類・有機酸類,或此等之鹽(鈉、鉀、鈣、鎂等之鹽)等之食品添加物之釀造醋也適合作為本發明之釀造醋。且,「膨脹度」意指含在口並飲入,到味道有後進為止之香味總量。且,本說明書中,「包含釀造醋之無酒精啤酒風味飲料」,或製造步驟中「添加釀造醋」意指至少一部分使用釀造醋之型態。亦即,藉由發酵食品等之素材所生成之釀造醋或添加釀造醋時以外,在作為釀造醋與釀造醋以外之成分之混合物添加時,也相當於「包含釀造醋之無酒精啤酒風味飲料」、「添加釀造醋」。也有考慮例如完全不包含由釀造所生成之乙酸,亦即冰乙酸或由化學合成所製造之合成乙酸與釀造醋併用時等。In the non-alcoholic beer-flavored beverage of the present invention, the amount of acetic acid can be adjusted by fermentation in the production process, or by adding acetic acid or a material containing acetic acid. It is better to make brewed vinegar from fermented food and other materials, or to add brewed vinegar to adjust. In this specification, "brewed vinegar" means including acetic acid produced (brewed) by microorganisms such as yeast, acetic acid bacteria, and lactic acid bacteria. For example, the Ministry of Agriculture, Forestry and Fisheries Announcement No. 811 of the Japanese Agriculture and Forestry Standards (JAS) on June 8, Showa 54 (final correction: Ministry of Agriculture, Forestry and Fisheries Announcement No. 1,506 on October 16, 2010) ) of brewed vinegar and table vinegar, etc., recorded in the Fair Competition Statute and Enforcement Rules (Enforced on May 18, 2013), although grain vinegar, fruit vinegar, rice vinegar, rice black vinegar, apple vinegar, etc. Vinegar, grape vinegar, etc. are also equivalent to brewing vinegar, but are not limited thereto. Acetic acid produced (brewed) by microorganisms such as yeast, acetic acid bacteria, and lactic acid bacteria may also be included in a part, and acetic acid produced by using acetic acid bacteria or lactic acid bacteria in the manufacturing process may also be included, but the general beer production method (yeast (fermentation) generated acetic acid is not included in the brewed vinegar of the present invention. And, mixing water or other foods or materials in these brewed vinegars is also called the brewed vinegar of the present invention. For example, brewed vinegar mixed with food materials such as sugars or plant extracts, or food additives such as nucleic acids and organic acids, or their salts (salts of sodium, potassium, calcium, magnesium, etc.) is also suitable as the present invention of brewing vinegar. In addition, the "overrun" means the total amount of flavor until the taste is retarded after being contained in the mouth and drunk. In addition, in this specification, "non-alcoholic beer-flavored beverage containing brewed vinegar" or "adding brewed vinegar" in the production process means that at least a part of brewed vinegar is used. That is, when fermented vinegar produced from materials such as fermented foods or when brewed vinegar is added, when added as a mixture of brewed vinegar and components other than brewed vinegar, it is also equivalent to "non-alcoholic beer-flavored beverages containing brewed vinegar" ", "Add brewed vinegar". There are also considerations, such as completely excluding acetic acid produced by brewing, that is, glacial acetic acid, or when synthetic acetic acid produced by chemical synthesis is used in combination with brewed vinegar.

乙酸中之釀造醋之比例以抑制酸臭味之觀點及賦予膨脹度之觀點來看,較佳為10質量%以上,再較佳為20質量%以上,更較佳為30質量%以上,更較佳為40質量%以上,更較佳為50質量%以上,更較佳為60質量%以上,更較佳為70質量%以上,更較佳為80質量%以上,更較佳為90質量%以上,最佳為100質量%。The proportion of brewed vinegar in acetic acid is preferably at least 10% by mass, more preferably at least 20% by mass, still more preferably at least 30% by mass, and even more preferably from the viewpoint of suppressing sour odor and imparting swelling. Preferably at least 40% by mass, more preferably at least 50% by mass, more preferably at least 60% by mass, more preferably at least 70% by mass, more preferably at least 80% by mass, more preferably at least 90% by mass More than %, the best is 100% by mass.

釀造醋之酸度並無限定,但有舉例如0.5以上,1.0以上,1.2以上,1.4以上,1.6以上,1.8以上,或8.0以下,7.5以下,7.0以下,6.5以下者。且,能夠設定成此等之值任意組合之範圍。且,以酸臭味之抑制之觀點來看,使用鈉量較多之釀造醋較佳。本發明中之鈉也包含以鈉離子、鈉鹽等之形態存在之情形。釀造醋中之鈉含量較佳為100質量ppm以上,再較佳為200質量ppm以上,更較佳為300質量ppm以上,更較佳為400質量ppm以上,更較佳為450質量ppm以上,更較佳為500質量ppm以上,更較佳為550質量ppm以上,更較佳為600質量ppm以上。且,以若多量添加,會賦予澀味或硬度之觀點來看,較佳為1000質量ppm以下,再較佳為900質量ppm以下,更較佳為800質量ppm以下,更較佳為700質量ppm以下。且,能夠設定成此等之值任意組合之範圍。本說明書中,啤酒風味飲料之酸度是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集,2013年增補改訂)之「8.6 酸度」中記載之方法來測定。本說明書中,釀造醋中之鈉之含量是根據(https://www.fukushihoken.metro.tokyo.lg.jp/shokuhin/hyouji/kyouzai/files/eiyouseibun_handbook.pdf)p.13中記載之原子吸光光度法來測定。The acidity of brewed vinegar is not limited, but examples include 0.5 or more, 1.0 or more, 1.2 or more, 1.4 or more, 1.6 or more, 1.8 or more, or 8.0 or less, 7.5 or less, 7.0 or less, 6.5 or less. And, it is possible to set the range in which these values are combined arbitrarily. Also, from the viewpoint of suppression of sour odor, it is preferable to use brewed vinegar with a large amount of sodium. Sodium in the present invention also includes cases where it exists in the form of sodium ions, sodium salts, and the like. The sodium content in the brewed vinegar is preferably at least 100 mass ppm, more preferably at least 200 mass ppm, more preferably at least 300 mass ppm, still more preferably at least 400 mass ppm, still more preferably at least 450 mass ppm, More preferably, it is 500 mass ppm or more, More preferably, it is 550 mass ppm or more, More preferably, it is 600 mass ppm or more. Also, from the viewpoint of imparting astringent taste or hardness when added in large amounts, it is preferably at most 1000 mass ppm, more preferably at most 900 mass ppm, still more preferably at most 800 mass ppm, still more preferably at most 700 mass ppm below ppm. And, it is possible to set the range in which these values are combined arbitrarily. In this manual, the acidity of beer-flavored beverages is based on "8.6 Acidity" of the revised BCOJ Beer Analysis Method (published by the Japan Brewers Association, compiled by the International Technical Committee of the Beer Brewing Association [Analysis Committee], supplemented and revised in 2013) method to measure. In this manual, the content of sodium in brewed vinegar is based on the atomic absorption light described in (https://www.fukushihoken.metro.tokyo.lg.jp/shokuhin/hyouji/kyouzai/files/eiyouseibun_handbook.pdf) p.13 Measured photometrically.

使本發明之無酒精啤酒風味飲料中含有釀造醋,尤其是使其含有鈉量較多之釀造醋,能夠賦予酸臭味之抑制之機制尚不確定,但推測鈉會具有pH值之緩衝作用,因此包含鈉之溶液在口腔內較難引起pH值之變化,能夠抑制乙酸之過量揮發,並抑制酸臭味。The non-alcoholic beer-flavored beverage of the present invention contains brewed vinegar, especially brewed vinegar with a large amount of sodium, which can suppress the sour odor. It is not certain, but it is speculated that sodium will have a buffering effect on the pH value. , Therefore, it is difficult for the solution containing sodium to cause pH changes in the oral cavity, and it can inhibit the excessive volatilization of acetic acid and suppress the sour odor.

本發明之無酒精啤酒風味飲料之pH值以賦予收斂感並抑制酸臭味之觀點來看,較佳為pH值2.8以上,再較佳為pH值2.9以上,更較佳為pH值3.0以上,更較佳為pH值3.1以上,更較佳為pH值3.2以上,更較佳為pH值3.3以上,更較佳為pH值3.4以上,更較佳為pH值3.5以上。且,以降低收斂感賦予效果之觀點來看,較佳為pH值4.2以下,再較佳為pH值4.1以下,更較佳為pH值4.0以下,更較佳為pH值3.9以下,更較佳為pH值3.8以下。pH值之調整能夠適當地設定乙酸之量、稀釋水或碳酸水之添加、原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之種類(乳酸、磷酸、蘋果酸、酒石酸、檸檬酸等)、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。本說明書中,無酒精啤酒風味飲料之pH值是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂之8.7 pH值中記載之方法來測定。The pH of the non-alcoholic beer-flavored beverage of the present invention is preferably pH 2.8 or higher, more preferably pH 2.9 or higher, and still more preferably pH 3.0 or higher, from the viewpoint of imparting an astringent feeling and suppressing sour odor , more preferably a pH value of 3.1 or higher, more preferably a pH value of 3.2 or higher, more preferably a pH value of 3.3 or higher, more preferably a pH value of 3.4 or higher, and more preferably a pH value of 3.5 or higher. And, from the viewpoint of reducing the effect of imparting astringent feeling, the pH value is preferably at most 4.2, more preferably at most pH 4.1, still more preferably at most pH 4.0, still more preferably at most pH 3.9, and even more preferably at most pH 4.0. Preferably, the pH value is below 3.8. The adjustment of pH value can properly set the amount of acetic acid, the addition of dilution water or carbonated water, the type of raw materials (malt, corn flour, sugar solution, etc.), the amount of raw materials, the type of enzyme, the amount of enzyme added, the amount of enzyme Adding time point, saccharification time in the preparation tank, protein decomposition time in the preparation tank, pH value in the preparation tank, pH value in the preparation step (wort production step after malt input to yeast addition), pH value The type of acid used for adjustment (lactic acid, phosphoric acid, malic acid, tartaric acid, citric acid, etc.), the amount of acid added for pH adjustment, and the time point of pH adjustment (during preparation, during fermentation, at the end of fermentation) time, before beer filtration, after beer filtration, etc.), the set temperature and holding time of each temperature zone when preparing wort (including saccharification), the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, Fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) are carried out. In this manual, the pH value of non-alcoholic beer-flavored beverages is based on the revised BCOJ Beer Analysis Method (published by the Japan Brewing Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewery Association [Analysis Committee] 8.7 pH value recorded method to measure.

本說明書中「啤酒風味飲料」意指如具有啤酒之風味之含酒精或無酒精之碳酸飲料。亦即,本說明書之啤酒風味飲料,只要沒有特別限定時,包含所有啤酒風味之碳酸飲料。本發明中係關於「無酒精啤酒風味飲料」。本說明書中,「無酒精啤酒風味飲料」意指酒精度數未滿1v/v%之啤酒風味飲料。本發明之無酒精啤酒風味飲料只要沒有特別限定時,無論有無酵母之發酵步驟,亦包含具有啤酒風味之任一種無酒精之碳酸飲料。亦即,本發明之無酒精啤酒風味飲料,只要是最終製品中之乙醇含量未滿1v/v%,亦可為發酵無酒精啤酒風味飲料,亦可為非發酵無酒精啤酒風味飲料。發酵無酒精啤酒風味飲料亦可為經過使用頂部發酵酵母之發酵步驟所釀造之頂部發酵無酒精啤酒風味飲料,亦可為經過使用底部發酵酵母之發酵步驟所釀造之底部發酵無酒精啤酒風味飲料。發酵時亦可使用生成酒精之酵母(酵母菌)或野生酵母(酒香酵母菌等),亦可使用不會生成酒精之酵母(酵母菌等)、野生酵母(酒香酵母菌等)、進行乳酸發酵或葡萄糖酸發酵之菌等。發酵無酒精啤酒風味飲料時,亦可以添加酵母,停止發酵使其未滿1v/v%之方法,或發酵後經過去酒精步驟使其未滿1v/v%之方法來製造。The term "beer-flavored beverage" in this specification refers to alcoholic or non-alcoholic carbonated beverages that have the flavor of beer. That is, the beer-flavored drinks in this specification include all beer-flavored carbonated drinks unless otherwise specified. The present invention relates to "non-alcoholic beer-flavored beverage". In this manual, "non-alcoholic beer-flavored beverage" means a beer-flavored beverage with an alcohol content of less than 1v/v%. Unless otherwise specified, the non-alcoholic beer-flavored beverage of the present invention includes any non-alcoholic carbonated beverage with beer flavor, regardless of whether there is a yeast fermentation step. That is, the non-alcoholic beer-flavored beverage of the present invention may be a fermented non-alcoholic beer-flavored beverage or a non-fermented non-alcoholic beer-flavored beverage as long as the ethanol content in the final product is less than 1v/v%. The fermented non-alcoholic beer-flavored beverage may also be a top-fermented non-alcoholic beer-flavored beverage brewed through a fermentation step using top-fermenting yeast, or may be a bottom-fermented non-alcoholic beer-flavored beverage brewed through a fermentation step using bottom-fermented yeast. Alcohol-producing yeast (saccharomyces) or wild yeast (brettanomyces, etc.) can also be used for fermentation, and yeast (saccharomyces, etc.) that do not produce alcohol, wild yeast (brettanomyces, etc.) Lactic acid fermentation or gluconic acid fermentation bacteria, etc. When fermenting non-alcoholic beer-flavored beverages, it can also be produced by adding yeast, stopping the fermentation to make it less than 1v/v%, or going through a de-alcohol step after fermentation to make it less than 1v/v%.

本發明之無酒精啤酒風味飲料之原麥汁萃取物(O-Ex)濃度較佳能夠設為14.0w/w%以下,再較佳為13.5w/w%以下,更較佳為13.0w/w%以下,更較佳為12.5w/w%以下,更較佳為12.0w/w%以下,更較佳為11.5w/w%以下,更較佳為11.0w/w%以下,更較佳為10.5w/w%以下,更較佳為10.0w/w%以下,更較佳為9.5w/w%以下,更較佳為9.0w/w%以下,且較佳為4.0w/w%以上,再較佳為4.5w/w%以上,更較佳為5.0w/w%以上,更較佳為5.5w/w%以上,更較佳為6.0w/w%以上,更較佳為6.5w/w%以上,更較佳為7.0w/w%以上,更較佳為7.5w/w%以上,更較佳為8.0w/w%以上,更較佳為8.5w/w%以上。本說明書中,原麥汁萃取物(O-Ex)濃度是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂中記載之方法來測定。原麥汁萃取物(O-Ex)濃度之調整能夠適當地設定稀釋水或碳酸水之添加、原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、麥汁過濾之時間、麥汁過濾之pH值、煮沸時間、煮沸溫度等來進行。The concentration of the original wort extract (O-Ex) of the non-alcoholic beer-flavored beverage of the present invention can preferably be set below 14.0w/w%, more preferably below 13.5w/w%, more preferably below 13.0w/w% w% or less, more preferably 12.5w/w% or less, more preferably 12.0w/w% or less, more preferably 11.5w/w% or less, more preferably 11.0w/w% or less, more preferably Preferably less than 10.5w/w%, more preferably less than 10.0w/w%, more preferably less than 9.5w/w%, more preferably less than 9.0w/w%, and more preferably less than 4.0w/w % or more, more preferably 4.5w/w% or more, more preferably 5.0w/w% or more, more preferably 5.5w/w% or more, more preferably 6.0w/w% or more, more preferably 6.5w/w% or more, more preferably 7.0w/w% or more, more preferably 7.5w/w% or more, more preferably 8.0w/w% or more, more preferably 8.5w/w% above. In this manual, the concentration of original wort extract (O-Ex) is based on the revised BCOJ Beer Analysis Method (published by the Japan Brewing Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewing Association [Analysis Committee] The adjustment of the concentration of the original wort extract (O-Ex) can properly set the addition of dilution water or carbonated water, the type of raw materials (malt, corn flour, sugar liquid, etc.), the amount of raw materials, wort filtration Time, pH value of wort filtration, boiling time, boiling temperature, etc.

本發明之無酒精啤酒風味飲料中,最終外觀發酵度(LA)能夠設為80%以下,75%以下,70%以下,68%以下,66%以下,64%以下,62%以下,60%以下,58%以下,56%以下,54%以下,53%以下,52%以下。且,能夠設為20%以上,22%以上,23%以上,24%以上,26%以上,28%以上,30%以上,32%以上,34%以上,36%以上,38%以上,40%以上,42%以上,44%以上,46%以上。本說明書中,最終外觀發酵度(LA)是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂中記載之方法來測定。最終外觀發酵度(LA)之調整能夠適當地設定原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、酵素之種類、酵素(亦包含糖質分解酵素、異性化酵素等)之添加量、酵素之添加之時間點、糖化時間、糖化時之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、麥汁過濾之時間、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the final appearance fermentation degree (LA) can be set to 80% or less, 75% or less, 70% or less, 68% or less, 66% or less, 64% or less, 62% or less, or 60% Below, below 58%, below 56%, below 54%, below 53%, below 52%. Furthermore, it can be set to 20% or more, 22% or more, 23% or more, 24% or more, 26% or more, 28% or more, 30% or more, 32% or more, 34% or more, 36% or more, 38% or more, 40% or more More than %, more than 42%, more than 44%, more than 46%. In this manual, the final appearance degree of fermentation (LA) is measured in accordance with the revised BCOJ Beer Analysis Method (published by the Japan Brewers Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewers Association [Analysis Committee]. Final The adjustment of appearance fermentation degree (LA) can properly set the types of raw materials (malt, corn flour, sugar liquid, etc.), the amount of raw materials, the types of enzymes, and the levels of enzymes (including glycolytic enzymes, heterosexualizing enzymes, etc.) Addition amount, timing of enzyme addition, saccharification time, pH value during saccharification, pH value in preparation steps (wort production steps from malt input to yeast addition), wort filtration time, wort preparation According to the setting temperature and holding time of each temperature zone during the time (including the time of saccharification), etc.

本發明之無酒精啤酒風味飲料中,酒精度數(酒精含量)較佳為未滿1.0v/v%,再較佳為0.95v/v%以下,更較佳為0.90v/v%以下,更較佳為0.85v/v%以下,更較佳為0.80v/v%以下,更較佳為0.75v/v%以下,更較佳為0.70v/v%以下,更較佳為0.60v/v%以下,更較佳為0.50v/v%以下,更較佳為0.40v/v%以下,更較佳為0.30v/v%以下,更較佳為0.20v/v%以下,更較佳為0.10v/v%以下,更較佳為0.05v/v%以下。本說明書中,酒精度數(酒精含量)是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂中記載之方法來測定。酒精度數之調整能夠適當地設定稀釋水或碳酸水之添加、發酵前液之糖組成、酵母之種類、酵母之量、釀造酒精之添加、蒸留酒精之添加等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the alcohol content (alcohol content) is preferably less than 1.0v/v%, more preferably less than 0.95v/v%, more preferably less than 0.90v/v%, and even more preferably Preferably less than 0.85v/v%, more preferably less than 0.80v/v%, more preferably less than 0.75v/v%, more preferably less than 0.70v/v%, more preferably less than 0.60v/v% V% or less, more preferably 0.50v/v% or less, more preferably 0.40v/v% or less, more preferably 0.30v/v% or less, more preferably 0.20v/v% or less, more preferably It is preferably at most 0.10v/v%, more preferably at most 0.05v/v%. In this manual, the alcohol content (alcohol content) is measured in accordance with the revised BCOJ Beer Analysis Method (published by the Japan Brewers Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewing Association [Analysis Committee]. Alcohol content The adjustment can be performed by appropriately setting the addition of dilution water or carbonated water, the sugar composition of the pre-fermentation liquid, the type of yeast, the amount of yeast, the addition of brewing alcohol, the addition of distilled alcohol, and the like.

本發明之無酒精啤酒風味飲料之色度能夠設為例如1.0EBC以上,3.0EBC以上,5.0EBC以上,6.0EBC以上,7.0EBC以上,8.0EBC以上,9.0EBC以上,10.0EBC以上,15.0EBC以上,20.0EBC以上,且能夠設為40.0EBC以下,30.0EBC以下。本說明書中,色度是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂之8.8 色度中記載之方法來測定。色度之調整能夠適當地設定稀釋水或碳酸水之添加、原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、糖化時之溫度、糖化時之pH值、糖化之時間、麥汁過濾之時間、麥汁過濾之pH值、煮沸時間、煮沸溫度、焦糖色素等之色素成分之量、啤酒過濾之形式(矽藻土過濾、各種膜過濾等)、啤酒過濾之過濾量等來進行。The chromaticity of the non-alcoholic beer-flavored beverage of the present invention can be, for example, 1.0 EBC or higher, 3.0 EBC or higher, 5.0 EBC or higher, 6.0 EBC or higher, 7.0 EBC or higher, 8.0 EBC or higher, 9.0 EBC or higher, 10.0 EBC or higher, 15.0 EBC or higher , 20.0EBC or more, and can be set to 40.0EBC or less, 30.0EBC or less. In this specification, the chromaticity is measured according to the revised BCOJ beer analysis method (published by the Japan Brewing Association, supplemented and revised in 2013 by the International Technical Committee of the Beer Brewing Association [Analysis Committee] and compiled by the method described in 8.8 Chromaticity. Chromaticity The adjustment can properly set the addition of dilution water or carbonated water, the type of raw materials (malt, corn flour, sugar solution, etc.), the amount of raw materials, the temperature during mashing, the pH value during mashing, the time of mashing, wort Filtration time, pH value of wort filtration, boiling time, boiling temperature, amount of pigment components such as caramel pigment, form of beer filtration (diatomaceous earth filtration, various membrane filtration, etc.), filtration volume of beer filtration, etc. conduct.

本發明之無酒精啤酒風味飲料之苦味價能夠設為例如5BUs以上,10BUs以上,15BUs以上,18BUs以上,20BUs以上,23BUs以上,25BUs以上,且能夠設為40BUs以下,35BUs以下,32BUs以下,30BUs、28BUs以下。本說明書中,苦味價是根據「BCOJ啤酒分析法(2004.11.1 改訂版)8.15 苦味價」之項中記載之方法來測定。具體來說,於脫氣後之樣品中添加酸之後,以異辛烷萃取,藉由將異辛烷作為對照,並以275nm計測所得之異辛烷層之吸光度,乘以因數後,得到苦味價(BU)。苦味價與飲料中包含之異α酸含量為正相關。藉由將啤酒花中包含之α酸異性化,得到異α酸。控制異α酸之量時,能夠藉由啤酒花品種、啤酒花之使用量、啤酒花之添加時間點、啤酒花之添加時之溫度及其溫度帶中之保持時間、啤酒花添加時之pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等、啤酒過濾之條件、稀釋水之添加、碳酸水之添加等來調整。且,能夠藉由市售之啤酒花加工品(異α酸等)等來調整,能夠藉由量、種類、添加時間點、添加時之溫度及其溫度帶中之保持時間、添加時之pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等、啤酒過濾之條件、稀釋水之添加、碳酸水之添加等來調整。啤酒花亦可使用成為片狀之片狀啤酒花,亦可使用萃取物化之萃取物啤酒花,亦可使用啤酒花苞。啤酒花及啤酒花加工品亦可單獨使用或併用。The bitterness value of the non-alcoholic beer-flavored beverage of the present invention can be, for example, 5 BUs or more, 10 BUs or more, 15 BUs or more, 18 BUs or more, 20 BUs or more, 23 BUs or more, 25 BUs or more, and can be 40 BUs or less, 35 BUs or less, 32 BUs or less, and 30 BUs , Below 28BUs. In this manual, the bitterness value is measured according to the method described in "BCOJ beer analysis method (2004.11.1 revised edition) 8.15 Bitterness value". Specifically, after adding acid to the degassed sample, extract it with isooctane, use isooctane as a control, and measure the absorbance of the obtained isooctane layer at 275nm, multiply by a factor, and obtain a bitter taste Price (BU). The bitterness value is positively correlated with the amount of iso-alpha acids contained in the beverage. Iso-alpha acids are obtained by iso-alpha acids contained in hops. When controlling the amount of iso-alpha acid, it can be controlled by the variety of hops, the amount of hops used, the time point of adding hops, the temperature when adding hops and the retention time in the temperature zone, the pH value when adding hops, and the pre-fermentation liquid. The original extract concentration, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting , carbon dioxide concentration, etc.), etc., the conditions of beer filtration, the addition of dilution water, the addition of carbonated water, etc. to adjust. In addition, it can be adjusted with commercially available processed hops (isoalpha acid, etc.), and can be adjusted by the amount, type, time point of addition, temperature at the time of addition, retention time in the temperature range, and pH value at the time of addition. , the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation Time, pressure setting, carbon dioxide concentration, etc.), beer filtration conditions, addition of dilution water, addition of carbonated water, etc. to adjust. The hops can also be flaked hops, extracted hops, or hop buds. Hops and hop processed products can also be used alone or in combination.

本發明之無酒精啤酒風味飲料中,全氮量能夠設為例如10mg/100mL以上,15mg/100mL以上,20mg/100mL以上,25mg/100mL以上,30mg/100mL以上,35mg/100mL以上,40mg/100mL以上,45mg/100mL以上,50mg/100mL以上,且能夠設為100mg/100mL以下,95mg/100mL以下,90mg/100mL以下,85mg/100mL以下,80mg/100mL以下,75mg/100mL以下,70mg/100mL以下。全氮量為蛋白質、胺基酸等之所有氮化合物之總量。本說明書中,全氮量是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂)8.9 全氮中記載之方法來測定。全氮量之調整能夠適當地設定稀釋水或碳酸水之添加、原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、酵素之種類、酵素(亦包含蛋白質分解酵素等)之添加量、酵素反應時之溫度、酵素之添加之時間點、準備槽中之蛋白質分解時間、準備槽中之pH值、準備槽中之溫度、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、準備步驟中之溫度、麥汁過濾時之溫度、麥汁過濾之時間、麥汁過濾時之pH值、麥汁過濾時所使用之噴射水之量、調製麥汁時之各溫度區域之設定溫度及保持時間、煮沸步驟中之煮沸時間及pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等、啤酒過濾之條件來進行。In the non-alcoholic beer-flavored beverage of the present invention, the amount of total nitrogen can be, for example, 10 mg/100 mL or more, 15 mg/100 mL or more, 20 mg/100 mL or more, 25 mg/100 mL or more, 30 mg/100 mL or more, 35 mg/100 mL or more, 40 mg/100 mL or more Above, above 45mg/100mL, above 50mg/100mL, and can be set below 100mg/100mL, below 95mg/100mL, below 90mg/100mL, below 85mg/100mL, below 80mg/100mL, below 75mg/100mL, below 70mg/100mL . The total nitrogen content is the total amount of all nitrogen compounds such as proteins and amino acids. In this instruction manual, the total nitrogen content is measured according to the method described in 8.9 Total Nitrogen of the revised BCOJ Beer Analysis Method (published by the Japan Brewers Association, edited by the International Technical Committee of Beer Brewing Association [Analysis Committee] in 2013). The adjustment of the amount of total nitrogen can properly set the addition of dilution water or carbonated water, the type of raw materials (malt, corn flour, sugar liquid, etc.), the amount of raw materials, the type of enzymes, and the amount of enzymes (including proteolytic enzymes, etc.) Addition amount, temperature during enzyme reaction, time point of enzyme addition, protein decomposition time in the preparation tank, pH value in the preparation tank, temperature in the preparation tank, preparation steps (malt after malt input to before yeast addition) The pH value in the juice making step), the temperature in the preparation step, the temperature during the wort filtration, the time of the wort filtration, the pH value during the wort filtration, the amount of spray water used in the wort filtration, the modulation of the wort The set temperature and holding time of each temperature zone when juicing, the boiling time and pH value in the boiling step, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, Yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) and other conditions for beer filtration.

本發明之無酒精啤酒風味飲料中,總多酚量能夠為10質量ppm以上,20質量ppm以上,30質量ppm以上,40質量ppm以上,50質量ppm以上,60質量ppm以上,70質量ppm以上,80質量ppm以上,90質量ppm以上,且能夠為200質量ppm以下,180質量ppm以下,150質量ppm以下,140質量ppm以下,130質量ppm以下,120質量ppm以下。本說明書中,總多酚量是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂)8.19 總多酚中記載之方法來測定。總多酚量之調整能夠適當地設定稀釋水或碳酸水之添加、原材料(包含麥芽等之多酚之原料)之種類、原材料之量、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽中之通氣時間(搗碎充氣等)、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、麥汁過濾之時間、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。且,本發明之啤酒風味飲料之總多酚量能夠藉由調整例如大麥麥芽、麥芽之外殼(穀皮)等之多酚含量較多之原材料之使用量來控制。具體來說,能夠藉由增加多酚含量較多之麥芽等之原材料之使用量來使總多酚量增加。In the non-alcoholic beer-flavored beverage of the present invention, the total polyphenol content can be at least 10 mass ppm, at least 20 mass ppm, at least 30 mass ppm, at least 40 mass ppm, at least 50 mass ppm, at least 60 mass ppm, and at least 70 mass ppm , 80 mass ppm or more, 90 mass ppm or more, and can be 200 mass ppm or less, 180 mass ppm or less, 150 mass ppm or less, 140 mass ppm or less, 130 mass ppm or less, 120 mass ppm or less. In this manual, the amount of total polyphenols is measured according to the method described in 8.19 Total Polyphenols of the revised BCOJ Beer Analysis Method (published by the Japan Brewing Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewing Association [Analysis Committee]) . The adjustment of the total polyphenol content can appropriately set the addition of dilution water or carbonated water, the type of raw materials (raw materials containing polyphenols such as malt), the amount of raw materials, the type of enzymes, the amount of enzymes added, and the amount of enzymes added. Time point, aeration time in the preparation tank (mashing and aeration, etc.), pH value in the preparation tank, pH value in the preparation step (wort production step after malt input and before yeast addition), wort filtration time , The set temperature and holding time of each temperature zone when preparing wort (including saccharification), the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species , the amount of yeast added, the number of yeast proliferation, the time point of yeast removal, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) and so on. Moreover, the total polyphenol content of the beer-flavored beverage of the present invention can be controlled by adjusting the amount of raw materials with high polyphenol content such as barley malt and malt husk (husk). Specifically, the total polyphenol content can be increased by increasing the amount of raw materials such as malt with a large polyphenol content.

本發明之無酒精啤酒風味飲料中,游離胺基酸態氮(FAN)之含量能夠設為1.0mg/100mL以上,2.0mg/ 100mL以上,3.0mg/100mL以上,4.0mg/100mL以上,5.0mg/100mL以上,6.0mg/100mL以上,7.0mg/100mL以上,8.0mg/100mL以上,9.0mg/100mL以上,10.0mg/ 100mL以上,且能夠設為20.0mg/100mL以下,19.0mg/ 100mL以下,18.0mg/100mL以下,17.0mg/100mL以下,16.0mg/100mL以下,15.0mg/100mL以下,14.0mg/100mL以下。本說明書中,FAN之含量是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂之8.18 游離胺基酸態氮中記載之方法來測定。FAN之含量之調整能夠適當地設定稀釋水或碳酸水之添加、原材料(麥芽、玉米粉、糖液等)之種類、原材料之量、酵素之種類、酵素(亦包含蛋白質分解酵素等)之添加量、酵素之添加之時間點、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、麥汁過濾之時間、調製麥汁時之各溫度區域之設定溫度及保持時間、煮沸步驟中之煮沸時間及pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the content of free amino acid nitrogen (FAN) can be set at 1.0mg/100mL or more, 2.0mg/100mL or more, 3.0mg/100mL or more, 4.0mg/100mL or more, 5.0mg /100mL or more, 6.0mg/100mL or more, 7.0mg/100mL or more, 8.0mg/100mL or more, 9.0mg/100mL or more, 10.0mg/100mL or more, and can be set to 20.0mg/100mL or less, 19.0mg/100mL or less, Below 18.0mg/100mL, below 17.0mg/100mL, below 16.0mg/100mL, below 15.0mg/100mL, below 14.0mg/100mL. In this manual, the content of FAN is based on the revised BCOJ Beer Analysis Method (published by the Japan Brewing Association, edited by the International Technical Committee of Beer Brewers Association [Analysis Committee] in 2013, supplemented and revised 8.18 Free amino acid nitrogen. The adjustment of FAN content can properly set the addition of dilution water or carbonated water, the type of raw materials (malt, corn flour, sugar solution, etc.), the amount of raw materials, the type of enzymes, enzymes (including proteolytic enzymes) etc.), the time point of enzyme addition, the protein decomposition time in the preparation tank, the pH value in the preparation tank, the pH value in the preparation step (wort production step after malt input and before yeast addition), The time of wort filtration, the set temperature and holding time of each temperature zone when preparing wort, the boiling time and pH value in the boiling step, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, the fermentation Conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) and so on.

本發明之無酒精啤酒風味飲料中,異α酸之含量能夠設為2.0質量ppm以上,4.0質量ppm以上,6.0質量ppm以上,7.0質量ppm以上,8.0質量ppm以上,9.0質量ppm以上,9.5質量ppm以上,10質量ppm以上,且能夠設為40.0質量ppm以下,35.0質量ppm以下,30.0質量ppm以下,25.0質量ppm以下,20.0質量ppm以下,15.0質量ppm以下。本說明書中,異α酸之含量是根據改訂BCOJ啤酒分析法(公益財團法人日本釀造協會發行,啤酒酒造組合國際技術委員會[分析委員會]編集2013年增補改訂之8.25 異α酸、α酸中記載之方法來測定。異α酸之含量之調整能夠藉由啤酒花品種、啤酒花之使用量、啤酒花之添加時間點、啤酒花之添加時之溫度及其溫度帶中之保持時間、啤酒花添加時之pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等、啤酒過濾之條件、稀釋水之添加、碳酸水之添加等來調整。且,也能夠以市售之啤酒花加工品(異α酸等)等來調整,並能夠藉由量、種類、添加時間點、添加時之溫度及其溫度帶中之保持時間、添加時之pH值、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等、啤酒過濾之條件、稀釋水之添加、碳酸水之添加等來調整。啤酒花亦可使用成為片狀之片狀啤酒花,亦可使用萃取物化之萃取物啤酒花,亦可使用啤酒花苞。啤酒花及啤酒花加工品亦可單獨使用或併用。In the non-alcoholic beer-flavored beverage of the present invention, the content of the iso-alpha acid can be set at 2.0 mass ppm or more, 4.0 mass ppm or more, 6.0 mass ppm or more, 7.0 mass ppm or more, 8.0 mass ppm or more, 9.0 mass ppm or more, 9.5 mass ppm or more ppm or more, 10 mass ppm or more, and can be 40.0 mass ppm or less, 35.0 mass ppm or less, 30.0 mass ppm or less, 25.0 mass ppm or less, 20.0 mass ppm or less, 15.0 mass ppm or less. In this manual, the content of iso-alpha acid is based on the revised BCOJ beer analysis method (published by the Japan Brewers Association, supplemented and revised in 2013 by the International Technical Committee of Beer Brewing Association [Analysis Committee] 8.25 Iso-alpha acid and alpha acid. The method to determine. The content of iso-alpha acid can be adjusted by the hop variety, the amount of hops used, the time point of adding hops, the temperature when adding hops and the holding time in the temperature zone, and the pH value when adding hops , the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), beer filtration conditions, addition of dilution water, addition of carbonated water, etc. It can also be adjusted with commercially available processed hops (isoalpha acid, etc.), And it can be determined by the amount, type, time point of addition, temperature at the time of addition and retention time in the temperature zone, pH value at the time of addition, original extract concentration of the pre-fermentation liquid, original extract concentration in the fermentation step, fermentation Conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), beer filtration conditions, addition of dilution water , the addition of carbonated water, etc. The hops can also be used in flaked hops, extract hops can also be used, and hop buds can also be used. Hops and hop processed products can also be used alone or in combination.

本發明之無酒精啤酒風味飲料中含有乙酸乙酯較佳。作為乙酸乙酯之含量,能夠設為0.1質量ppm以上,0.2質量ppm以上,0.3質量ppm以上,0.4質量ppm以上,0.5質量ppm以上,0.6質量ppm以上,0.7質量ppm以上,0.8質量ppm以上,0.9質量ppm以上,1.0質量ppm以上,2.0質量ppm以上,3.0質量ppm以上,4.0質量ppm以上,5.0質量ppm以上,6.0質量ppm以上,7.0質量ppm以上,8.0質量ppm以上,9.0質量ppm以上,10.0質量ppm以上,11.0質量ppm以上,12.0質量ppm以上,13.0質量ppm以上,14.0質量ppm以上,15.0質量ppm以上,且能夠成為60.0質量ppm以下,50.0質量ppm以下,45.0質量ppm以下,40.0質量ppm以下。本說明書中,乙酸乙酯之含量是藉由氣體色層層析來測定。乙酸乙酯之含量之調整能夠適當地設定稀釋水或碳酸水之添加、含乙酸乙酯之香料之添加、含乙酸乙酯之香料之量、含乙酸乙酯之原料之量、含乙酸乙酯之原料之添加、包含乙酸乙酯之原材料之種類、乙酸乙酯之基質之量、成為乙酸乙酯之基質之原材料之量、原材料之量、原材料之種類、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。The non-alcoholic beer-flavored beverage of the present invention preferably contains ethyl acetate. The content of ethyl acetate can be 0.1 mass ppm or more, 0.2 mass ppm or more, 0.3 mass ppm or more, 0.4 mass ppm or more, 0.5 mass ppm or more, 0.6 mass ppm or more, 0.7 mass ppm or more, 0.8 mass ppm or more, 0.9 mass ppm or more, 1.0 mass ppm or greater, 2.0 mass ppm or greater, 3.0 mass ppm or greater, 4.0 mass ppm or greater, 5.0 mass ppm or greater, 6.0 mass ppm or greater, 7.0 mass ppm or greater, 8.0 mass ppm or greater, 9.0 mass ppm or greater, 10.0 mass ppm or more, 11.0 mass ppm or greater, 12.0 mass ppm or greater, 13.0 mass ppm or greater, 14.0 mass ppm or greater, 15.0 mass ppm or greater, and can be 60.0 mass ppm or less, 50.0 mass ppm or less, 45.0 mass ppm or less, 40.0 mass ppm or less below ppm. In this specification, the content of ethyl acetate is determined by gas chromatography. The adjustment of the content of ethyl acetate can properly set the addition of dilution water or carbonated water, the addition of fragrance containing ethyl acetate, the amount of fragrance containing ethyl acetate, the amount of raw materials containing ethyl acetate, the amount of ethyl acetate containing The addition of raw materials, the type of raw materials including ethyl acetate, the amount of ethyl acetate substrate, the amount of raw materials that become the ethyl acetate substrate, the amount of raw materials, the type of raw materials, the type of enzyme, the amount of enzyme added, The timing of adding enzymes, the saccharification time of the preparation tank, the protein decomposition time in the preparation tank, the pH value in the preparation tank, the pH value in the preparation step (wort production step after malt input and before yeast addition), The amount of acid used for pH adjustment, the timing of pH adjustment (preparation, fermentation, end of fermentation, before beer filtration, after beer filtration, etc.), wort preparation (including saccharification) The set temperature and holding time of the temperature zone, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast Remove time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) etc. to carry out.

本發明之無酒精啤酒風味飲料中,乙酸異戊酯之含量能夠設為0.01質量ppm以上,0.02質量ppm以上,0.03質量ppm以上,0.04質量ppm以上,0.05質量ppm以上,0.06質量ppm以上,0.07質量ppm以上,0.08質量ppm以上,0.09質量ppm以上,0.1質量ppm以上,0.15質量ppm以上,0.20質量ppm以上,0.25質量ppm以上,0.30質量ppm以上,0.35質量ppm以上,0.40質量ppm以上,0.45質量ppm以上,0.50質量ppm以上,1.0質量ppm以上,2.0質量ppm以上,3.0質量ppm以上,4.0質量ppm以上,5.0質量ppm以上,且能夠設為20.0質量ppm以下,15.0質量ppm以下,10.0質量ppm以下。本說明書中,乙酸異戊酯之含量是根據氣體色層層析來測定。乙酸異戊酯之含量之調整能夠適當地設定稀釋水或碳酸水之添加、含乙酸異戊酯之香料之添加、含乙酸異戊酯之香料之量、含乙酸異戊酯之原料之量、含乙酸異戊酯之原料之添加、包含乙酸異戊酯之原材料之種類、乙酸異戊酯之基質之量、成為乙酸異戊酯之基質之原材料之量、原材料之量、原材料之種類、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the content of isoamyl acetate can be 0.01 mass ppm or more, 0.02 mass ppm or greater, 0.03 mass ppm or greater, 0.04 mass ppm or greater, 0.05 mass ppm or greater, 0.06 mass ppm or greater, 0.07 mass ppm or greater. Mass ppm or more, 0.08 mass ppm or greater, 0.09 mass ppm or greater, 0.1 mass ppm or greater, 0.15 mass ppm or greater, 0.20 mass ppm or greater, 0.25 mass ppm or greater, 0.30 mass ppm or greater, 0.35 mass ppm or greater, 0.40 mass ppm or greater, 0.45 Mass ppm or more, 0.50 mass ppm or more, 1.0 mass ppm or more, 2.0 mass ppm or more, 3.0 mass ppm or more, 4.0 mass ppm or more, 5.0 mass ppm or more, and can be set to 20.0 mass ppm or less, 15.0 mass ppm or less, 10.0 mass ppm below ppm. In this specification, the content of isoamyl acetate is measured by gas chromatography. The adjustment of the content of isoamyl acetate can appropriately set the addition of dilution water or carbonated water, the addition of fragrances containing isoamyl acetate, the amount of fragrances containing isoamyl acetate, the amount of raw materials containing isoamyl acetate, Addition of raw material containing isoamyl acetate, type of raw material containing isoamyl acetate, amount of base material for isoamyl acetate, amount of raw material used as base material for isoamyl acetate, amount of raw material, type of raw material, enzyme The type of enzyme, the amount of enzyme added, the time point of enzyme addition, the saccharification time of the preparation tank, the protein decomposition time in the preparation tank, the pH value in the preparation tank, the preparation steps (wort after the malt is put in and before the yeast is added) The pH value in the production process), the amount of acid added during pH adjustment, the time point of pH adjustment (preparation, fermentation, end of fermentation, before beer filtration, after beer filtration, etc.), wort preparation The set temperature and holding time of each temperature zone (including saccharification), the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition) amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) and so on.

本發明之無酒精啤酒風味飲料中,異戊醇之含量能夠設為1.0質量ppm以上,2.0質量ppm以上,3.0質量ppm以上,4.0質量ppm以上,5.0質量ppm以上,6.0質量ppm以上,7.0質量ppm以上,8.0質量ppm以上,9.0質量ppm以上,10.0質量ppm以上,15.0質量ppm以上,20.0質量ppm以上,30.0質量ppm以上,且能夠設為100.0質量ppm以下,90.0質量ppm以下,80.0質量ppm以下,70.0質量ppm以下,60.0質量ppm以下,50.0質量ppm以下,40.0質量ppm以下。本說明書中,異戊醇之含量係藉由氣體色層層析來測定。異戊醇之含量之調整能夠適當地設定稀釋水或碳酸水之添加、含異戊醇之香料之添加、含異戊醇之香料之量、含異戊醇之原料之量、含異戊醇之原料之添加、包含異戊醇之原材料之種類、異戊醇之基質之量、成為異戊醇之基質之原材料之量、原材料之量、原材料之種類、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the content of isoamyl alcohol can be set at 1.0 mass ppm or more, 2.0 mass ppm or more, 3.0 mass ppm or more, 4.0 mass ppm or more, 5.0 mass ppm or more, 6.0 mass ppm or more, 7.0 mass ppm or more ppm or more, 8.0 mass ppm or more, 9.0 mass ppm or more, 10.0 mass ppm or more, 15.0 mass ppm or more, 20.0 mass ppm or more, 30.0 mass ppm or more, and can be set to 100.0 mass ppm or less, 90.0 mass ppm or less, 80.0 mass ppm Below, 70.0 mass ppm or less, 60.0 mass ppm or less, 50.0 mass ppm or less, 40.0 mass ppm or less. In this specification, the content of isoamyl alcohol is determined by gas chromatography. The adjustment of the content of isoamyl alcohol can properly set the addition of dilution water or carbonated water, the addition of flavors containing isoamyl alcohol, the amount of flavors containing isoamyl alcohol, the amount of raw materials containing isoamyl alcohol, the amount of isoamyl alcohol containing The addition of raw materials, the type of raw materials containing isoamyl alcohol, the amount of the base of isoamyl alcohol, the amount of raw materials that become the base of isoamyl alcohol, the amount of raw materials, the type of raw materials, the type of enzyme, the amount of enzyme added, The timing of adding enzymes, the saccharification time of the preparation tank, the protein decomposition time in the preparation tank, the pH value in the preparation tank, the pH value in the preparation step (wort production step after malt input and before yeast addition), The amount of acid used for pH adjustment, the timing of pH adjustment (preparation, fermentation, end of fermentation, before beer filtration, after beer filtration, etc.), wort preparation (including saccharification) The set temperature and holding time of the temperature zone, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast Remove time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) etc. to carry out.

本發明之無酒精啤酒風味飲料中,己酸乙酯之含量能夠設為1.0質量ppb以上,2.0質量ppb以上,3.0質量ppb以上,4.0質量ppb以上,5.0質量ppb以上,6.0質量ppb以上,7.0質量ppb以上,8.0質量ppb以上,9.0質量ppb以上,10.0質量ppb以上,15.0質量ppb以上,20.0質量ppb以上,25.0質量ppb以上,30.0質量ppb以上,35.0質量ppb以上,40.0質量ppb以上,45.0質量ppb以上,50.0質量ppb以上,且能夠設為900.0質量ppb以下,800.0質量ppb以下,700.0質量ppb以下,650.0質量ppb以下,600.0質量ppb以下,550.0質量ppb以下,500.0質量ppb以下,450.0質量ppb以下,400.0質量ppb以下。本說明書中,己酸乙酯之含量係藉由氣體色層層析來測定。己酸乙酯之含量之調整能夠適當地設定稀釋水或碳酸水之添加、含己酸乙酯之香料之添加、含己酸乙酯之香料之量、含己酸乙酯之原料之量、含己酸乙酯之原料之添加、包含己酸乙酯之原材料之種類、己酸乙酯之基質之量、成為己酸乙酯之基質之原材料之量、原材料之量、原材料之種類、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the content of ethyl caproate can be 1.0 mass ppb or more, 2.0 mass ppb or greater, 3.0 mass ppb or greater, 4.0 mass ppb or greater, 5.0 mass ppb or greater, 6.0 mass ppb or greater, and 7.0 mass ppb or greater. Above mass ppb, above 8.0 mass ppb, above 9.0 mass ppb, above 10.0 mass ppb, above 15.0 mass ppb, above 20.0 mass ppb, above 25.0 mass ppb, above 30.0 mass ppb, above 35.0 mass ppb, above 40.0 mass ppb, 45.0 Mass ppb or more, 50.0 mass ppb or more, and can be set to 900.0 mass ppb or less, 800.0 mass ppb or less, 700.0 mass ppb or less, 650.0 mass ppb or less, 600.0 mass ppb or less, 550.0 mass ppb or less, 500.0 mass ppb or less, 450.0 mass Below ppb, below 400.0 mass ppb. In this specification, the content of ethyl hexanoate is determined by gas chromatography. The adjustment of the content of ethyl caproate can appropriately set the addition of dilution water or carbonated water, the addition of fragrance containing ethyl caproate, the amount of fragrance containing ethyl caproate, the amount of raw materials containing ethyl caproate, Addition of raw materials containing ethyl caproate, type of raw materials containing ethyl caproate, amount of base of ethyl caproate, amount of raw materials to be a base of ethyl caproate, amount of raw materials, type of raw materials, enzyme The type of enzyme, the amount of enzyme added, the time point of enzyme addition, the saccharification time of the preparation tank, the protein decomposition time in the preparation tank, the pH value in the preparation tank, the preparation steps (wort after the malt is put in and before the yeast is added) The pH value in the production process), the amount of acid added during pH adjustment, the time point of pH adjustment (preparation, fermentation, end of fermentation, before beer filtration, after beer filtration, etc.), wort preparation The set temperature and holding time of each temperature zone (including saccharification), the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition) amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) and so on.

本發明之無酒精啤酒風味飲料中,4-乙烯基癒創木醇(4VG)之含量能夠設為1μg/L以上,5μg/L以上,7μg/L以上,10μg/L以上,μg/L以上,15μg/L以上,20μg/L以上,且能夠設為500μg/L以下,450μg/L以下,400μg/L以下,350μg/L以下,300μg/L以下。本說明書中,4-乙烯基癒創木醇之含量係藉由氣體色層層析來測定。4-乙烯基癒創木醇之含量之調整能夠適當地設定稀釋水或碳酸水之添加、含4-乙烯基癒創木醇之香料之添加、含4-乙烯基癒創木醇之香料之量、含4-乙烯基癒創木醇之原料之量、含4-乙烯基癒創木醇之原料之添加、包含4-乙烯基癒創木醇之原材料之種類、4-乙烯基癒創木醇之基質(阿魏酸等)之量、成為4-乙烯基癒創木醇之基質(阿魏酸等)之原材料之量、原材料之量、原材料之種類、酵素之種類、酵素之添加量、酵素之添加之時間點、準備槽之糖化時間、準備槽中之蛋白質分解時間、準備槽中之pH值、準備步驟(麥芽投入後到酵母添加前之麥汁製造步驟)中之pH值、pH值調整時所使用之酸之添加量、pH值調整之時間點(準備時、發酵時、發酵結束時、啤酒過濾前、啤酒過濾後等)、調製麥汁時(包含糖化時)之各溫度區域之設定溫度及保持時間、發酵前液之原始萃取物濃度、發酵步驟中之原始萃取物濃度、發酵條件(氧濃度、通氣條件、酵母品種、酵母之添加量、酵母增殖數、酵母之去除時間點、發酵溫度、發酵時間、壓力設定、二氧化碳濃度等)等來進行。In the non-alcoholic beer-flavored beverage of the present invention, the content of 4-vinylguaiacol (4VG) can be set at least 1 μg/L, at least 5 μg/L, at least 7 μg/L, at least 10 μg/L, and at least μg/L , 15 μg/L or more, 20 μg/L or more, and can be set to 500 μg/L or less, 450 μg/L or less, 400 μg/L or less, 350 μg/L or less, 300 μg/L or less. In this specification, the content of 4-vinylguaiacol is determined by gas chromatography. The adjustment of the content of 4-vinyl guaiacol can properly set the addition of dilution water or carbonated water, the addition of 4-vinyl guaiacol-containing fragrance, the addition of 4-vinylguaiacol-containing fragrance Quantity, amount of raw materials containing 4-vinyl guaiacol, addition of raw materials containing 4-vinyl guaiacol, types of raw materials containing 4-vinyl guaiacol, 4-vinyl guaiacol Amount of base of wood alcohol (ferulic acid, etc.), amount of raw material used as base of 4-vinylguaiacol (ferulic acid, etc.), amount of raw material, type of raw material, type of enzyme, addition of enzyme Quantity, time point of adding enzyme, saccharification time in preparation tank, protein decomposition time in preparation tank, pH value in preparation tank, pH in preparation step (wort production step after malt input to yeast addition) pH value, the amount of acid used for pH adjustment, the time point of pH adjustment (preparation, fermentation, end of fermentation, before beer filtration, after beer filtration, etc.), wort preparation (including saccharification) The set temperature and holding time of each temperature zone, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, Yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) etc.

本發明之無酒精啤酒風味飲料中,主要原材料亦可同時使用水及麥芽,或亦可不使用麥芽。進而,亦可為使用啤酒花,另外,亦可使用保存料、甜味料、水溶性食物纖維、苦味料或苦味賦予劑、抗氧化劑、香料、酸味料、鹽類、烈酒等。In the non-alcoholic beer-flavored beverage of the present invention, water and malt may be used as main raw materials, or malt may not be used. Furthermore, hops may be used, and preservatives, sweeteners, water-soluble dietary fibers, bittering agents or bittering agents, antioxidants, fragrances, sour agents, salts, spirits, etc. may be used.

麥芽意指使大麥、小麥、裸麥、野燕麥、燕麥、薏仁、燕麥片等之麥類種子發芽並乾燥,並進行除根者,產地或品種亦可為任意者,為大麥麥芽較佳。 大麥麥芽為作為日本啤酒風味飲料之原料中最被一般使用之麥芽之一。大麥有二條大麥、六條大麥等之種類,但亦可使用任一者。進而,通常麥芽以外,能夠使用色麥芽等。且,使用色麥芽時,亦可適當地組合種類相異之色麥芽來使用,亦可使用一種色麥芽。 Malt means barley, wheat, rye, wild oats, oats, barley, oatmeal, and other wheat seeds that are germinated, dried, and rooted. The place of origin or variety can be arbitrary, and barley malt is preferred. Barley malt is one of the most commonly used malts as a raw material for Japanese beer-flavored beverages. There are types of barley such as double barley and six barley, but either one can be used. Furthermore, colored malt etc. can be used other than normal malt. In addition, when using colored malt, it is also possible to use a combination of different colored malts appropriately, or to use one kind of colored malt.

且,亦可使用麥芽以外之穀物、蛋白質、酵母萃取物、糖液等與麥芽併用。作為如此之穀物,有舉例如不相當於麥芽之麥類(大麥、小麥、裸麥、野燕麥、燕麥、薏仁、燕麥片等)、米(白米、玄米等)、玉米(玉米粉等)、高梁、馬鈴薯、豆(大豆、豌豆等)、蕎麥、蜀黍、小米、紫穗稗及由此等所得之澱粉、此等之萃取物(extract)等。此等中,使用玉米(玉米粉等)較佳。且,作為蛋白質,有舉出大豆蛋白質、豌豆蛋白質、酵母萃取物、此等之分解物等。In addition, grains other than malt, protein, yeast extract, sugar solution, etc. may be used in combination with malt. Examples of such grains include wheat (barley, wheat, rye, wild oats, oats, barley, oatmeal, etc.), rice (white rice, brown rice, etc.), corn (corn flour, etc.) that do not correspond to malt , sorghum, potato, bean (soybean, pea, etc.), buckwheat, sorghum, millet, barnyardgrass and starch obtained therefrom, and their extracts (extract), etc. Among these, it is preferable to use corn (corn flour, etc.). In addition, examples of protein include soybean protein, pea protein, yeast extract, and their decomposed products.

且,不使用麥芽時,有舉出使用含有碳源之液糖、麥芽以外之上述之穀物等作為含有胺基酸之材料(例如大豆蛋白等)之氮源之無酒精啤酒風味飲料。In addition, when malt is not used, non-alcoholic beer-flavored beverages using liquid sugar containing carbon sources, the above-mentioned grains other than malt, etc. as nitrogen sources of amino acid-containing materials (such as soybean protein, etc.) are exemplified.

作為啤酒花,有舉例如片狀啤酒花、粉末啤酒花、啤酒花萃取物等。且,使用之啤酒花亦可使用異化啤酒花、還原啤酒花等之啤酒花加工品。Examples of hops include flaky hops, powdered hops, and hop extracts. In addition, processed hops such as dissimilated hops and reduced hops may be used as the hops to be used.

作為保存料,有舉例如苯甲酸;苯甲酸鈉等之苯甲酸鹽;對氧苯甲酸丙酯、對氧苯甲酸丁酯等之苯甲酸酯;二碳酸二甲酯等。且,作為保存料,亦可使用強力Sanpureza(三榮源FFI股份有限公司製,苯甲酸鈉與苯甲酸丁酯之混合物)等之市售之製劑。此等之保存料亦可單獨使用,亦可併用2種以上。 保存料之摻混量較佳為5~1200質量ppm,再較佳為10~1100質量ppm,更較佳為15~1000質量ppm,再更較佳為20~900質量ppm。 Examples of preservatives include benzoic acid; benzoates such as sodium benzoate; benzoates such as propyl p-oxybenzoate and butyl p-oxybenzoate; and dimethyl dicarbonate. In addition, as a preservative, commercially available preparations such as Qiangli Sanpureza (manufactured by Saneiyuan FFI Co., Ltd., a mixture of sodium benzoate and butyl benzoate) can also be used. These preservatives may be used alone or in combination of two or more. The blending amount of the preservative is preferably 5-1200 mass ppm, more preferably 10-1100 mass ppm, more preferably 15-1000 mass ppm, still more preferably 20-900 mass ppm.

作為甜味料,有舉出將來自穀物之澱粉以酸或酵素等分解之市售之糖化液、市售之水飴等之糖類、三糖類以上之糖、糖醇、甜菊等之天然甜味料、人工甜味料等。此等之糖類之形態亦可為溶液等之液體,亦可為粉末等之固體。且,澱粉之原料穀物之種類、澱粉之純化方法及酵素或酸之水解等之處理條件,並無特別限制。例如藉由適當設定酵素或酸之水解之條件,亦可使用提高麥芽糖之比率之糖類。此外,能夠使用蔗糖、果糖、葡萄糖、麥芽糖、海藻糖、麥芽三糖及此等之溶液(糖液)等。亦能夠使用異麥芽糖、異麥芽三糖等之異性化後之糖等。且,作為人工甜味料,有舉例如阿斯巴甜、乙鮮舒泛鉀(乙鮮舒泛K)、三氯蔗糖、紐甜等。甜味料亦可單獨使用,亦可併用2種以上。Examples of sweeteners include natural sweeteners such as commercially available saccharification liquid obtained by decomposing starch derived from grains with acid or enzymes, sugars such as commercially available starch syrup, sugars of trisaccharides or more, sugar alcohols, and stevia. , artificial sweeteners, etc. The form of these saccharides may be a liquid such as a solution, or a solid such as a powder. In addition, there are no particular limitations on the types of grains used as raw materials for starch, starch purification methods, and treatment conditions such as enzyme or acid hydrolysis. For example, by appropriately setting conditions for enzyme or acid hydrolysis, it is also possible to use sugars that increase the ratio of maltose. In addition, sucrose, fructose, glucose, maltose, trehalose, maltotriose, and solutions (sugar solutions) thereof can be used. It is also possible to use isomaltose, isomaltotriose and the like after isotropic sugars. In addition, examples of artificial sweeteners include aspartame, acesulfame potassium (acesulfame K), sucralose, neotame, and the like. A sweetener may be used independently, or may use 2 or more types together.

作為水溶性食物纖維,有舉例如難消化性糊精、多醣類、大豆食物纖維、瓜爾膠分解物、果膠、葡甘聚醣 、海藻酸、昆布多醣、岩藻多糖、卡拉膠等,以安定性或安全性等之廣用性之觀點來看,為難消化性糊精或多醣類較佳。Examples of water-soluble dietary fiber include indigestible dextrin, polysaccharides, soybean dietary fiber, decomposed guar gum, pectin, glucomannan, alginic acid, laminarin, fucoidan, carrageenan, etc. , Indigestible dextrins or polysaccharides are preferred from the viewpoint of stability, safety, etc., and wide applicability.

作為苦味料或苦味賦予劑,並無特別限定,除了啤酒花以外,有舉例如迷迭香、荔枝、姬茴香、杜松實、鼠尾草、迷迭香、靈芝、月桂樹、苦木素、柑橘萃取物、苦樹萃取物、咖啡萃取物、茶萃取物、苦瓜萃取物、蓮胚芽萃取物、木蘆薈萃取物、迷迭香萃取物、荔枝萃取物、月桂萃取物、鼠尾草萃取物、姬茴香萃取物、柚皮苷、苦艾素、艾草、艾草萃取物等。The bittering agent or bitterness-imparting agent is not particularly limited, and examples include rosemary, lychee, fennel, juniper, sage, rosemary, ganoderma, laurel, bitterwood, and citrus extracts other than hops , bitter tree extract, coffee extract, tea extract, bitter gourd extract, lotus germ extract, aloe bark extract, rosemary extract, lychee extract, laurel extract, sage extract, fennel Extract, naringin, absinthin, wormwood, wormwood extract, etc.

作為抗氧化劑,並無特別限定,但能夠使用一般啤酒或發泡酒中作為抗氧化劑所使用者,有舉例如抗壞血酸、異抗壞血酸、維他命E等之生育酚類及兒茶素等。The antioxidant is not particularly limited, but it can be used as an antioxidant in general beer or sparkling wine, such as ascorbic acid, erythorbic acid, vitamin E and other tocopherols and catechins.

作為香料,並無特別限定,能夠使用一般的啤酒香料。啤酒香料是使用來給予如啤酒之風味、亦包含由發酵所產生之釀造成分等。作為啤酒香料,有舉出酯或高級醇或內酯類,有舉例如包含乙酸異戊酯、n-丙醇、異丁醇、乙醛、己酸乙酯、辛酸乙酯、異戊酯丙酸酯、沉香醇、香葉醇、檸檬醛、4-乙烯基癒創木醇(4-VG)、4-甲基-3-戊烯酸、2-甲基-2-戊烯酸、1,4-桉油酚、1,8-桉油酚、2,3-二乙基-5-甲基吡嗪、γ-癸內酯、γ-十一酸內酯、己酸乙酯、2-甲基酪酸乙酯、n-酪酸乙酯、月桂烯等之啤酒香料(使人想起似啤酒之香味之香料)。The flavor is not particularly limited, and general beer flavors can be used. Beer spices are used to give flavors such as beer, and also include brewing ingredients produced by fermentation, etc. Examples of beer flavors include esters, higher alcohols or lactones, such as isoamyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate, ethyl caprylate, isopentyl propyl esters, linalool, geraniol, citral, 4-vinylguaiacol (4-VG), 4-methyl-3-pentenoic acid, 2-methyl-2-pentenoic acid, 1 ,4-cineol, 1,8-cineol, 2,3-diethyl-5-methylpyrazine, γ-decalactone, γ-undecanoolactone, ethyl caproate, 2 - Beer flavors such as ethyl methyl butyrate, n-ethyl butyrate, myrcene (flavors reminiscent of beer-like aroma).

作為酸味料,只要是具有酸味之物質即可,並無特別限定,但有舉例如酒石酸、磷酸、檸檬酸、葡萄糖酸、乳酸、蘋果酸、植酸、琥珀酸、葡萄糖酸內酯或此等之鹽。此等之酸味料中,為酒石酸、磷酸、檸檬酸、葡萄糖酸、乳酸、蘋果酸、植酸、琥珀酸或此等之鹽較佳,為酒石酸、磷酸、檸檬酸、乳酸、或此等之鹽再較佳,此等之酸味料亦可單獨來使用,亦可併用2種以上。The sour agent is not particularly limited as long as it has a sour taste, and examples thereof include tartaric acid, phosphoric acid, citric acid, gluconic acid, lactic acid, malic acid, phytic acid, succinic acid, gluconolactone, and the like. of salt. Among these acidulants, tartaric acid, phosphoric acid, citric acid, gluconic acid, lactic acid, malic acid, phytic acid, succinic acid or their salts are preferred, and tartaric acid, phosphoric acid, citric acid, lactic acid, or their salts are preferred. Salt is more preferable, and these acidulants may be used alone or in combination of two or more.

本說明書中,烈酒意指將麥、米、蕎麥、玉米等之穀物作為原料,且使用麥芽或因應必要之酵素劑來糖化,且使用酵母使其發酵後,進而蒸餾所得之酒類。作為烈酒之原材料之穀物,為麥較佳。In this specification, "spirited liquor" refers to alcoholic beverages that use grains such as wheat, rice, buckwheat, and corn as raw materials, saccharify them with malt or necessary enzymes, ferment them with yeast, and then distill them. The grain used as the raw material of spirits is preferably wheat.

作為本發明之無酒精啤酒風味飲料之一型態,有舉出飲料被裝在容器中之形態。作為容器之例,有舉例如瓶、寶特瓶、罐,或桶,但尤其是以方便攜帶之觀點來看,為罐、瓶、寶特瓶較佳。且,使用無色透明之瓶或寶特瓶時,由於會被曝露在太陽光或螢光燈之光下,為有色之瓶、有色之寶特瓶、罐、或桶較佳。One of the forms of the non-alcoholic beer-flavored drink of the present invention includes a form in which the drink is contained in a container. Examples of the container include a bottle, a plastic bottle, a can, or a barrel, but especially from the viewpoint of portability, a can, a bottle, and a plastic bottle are preferable. Also, when using a colorless and transparent bottle or plastic bottle, since it will be exposed to sunlight or fluorescent light, a colored bottle, colored plastic bottle, can, or barrel is preferable.

本發明之無酒精啤酒風味飲料之麥芽比率能夠設為20%以上,25%以上,30%以上,35%以上,40%以上,42%以上,45%以上,且能夠設為100%以下,90%以下,80%以下,75%以下,70%以下,65%以下,60%以下,55%以下,52%以下,50%以下。本說明書中,麥芽比率意指根據平成30年4月1日為施行日之酒稅法及酒類行政關係法令等解釋通達所計算出之值。The malt ratio of the non-alcoholic beer-flavored beverage of the present invention can be 20% or more, 25% or more, 30% or more, 35% or more, 40% or more, 42% or more, 45% or more, and can be 100% or less , less than 90%, less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 55%, less than 52%, less than 50%. In this manual, the malt ratio refers to the value calculated based on the interpretation of the Liquor Tax Law and Liquor Administrative Relations Act, which took effect on April 1, 2013.

本發明之無酒精啤酒風味飲料之碳酸氣體之量以飲料之碳酸氣體壓來表示。典型來說,飲料之碳酸氣體壓之上限為5.0kg/cm 2、4.5kg/cm 2,或4.0kg/cm 2,下限為0.20kg/cm 2、0.50kg/cm 2,或1.0kg/cm 2,且亦可組合此等之上限及下限中之任一者。例如,飲料之碳酸氣體壓亦可為0.20kg/cm 2以上5.0kg/cm 2以下,0.50kg/cm 2以上4.5kg/cm 2以下,或1.0kg/cm 2以上4.0kg/cm 2以下。本說明書中,氣體壓意指去除特別狀況,容器內之氣體壓。壓力之測定能夠藉由使用將成為20℃之試料固定於氣體內壓計後,先打開氣體內壓計之活栓放出氣體,再將活栓關閉,搖動氣體內壓計,讀取指針到達一定位置之值之方法,或使用市售之氣體壓測定裝置來測定。 The amount of carbonic acid gas in the non-alcoholic beer-flavored beverage of the present invention is represented by the carbonic acid gas pressure of the beverage. Typically, the upper limit of the carbon dioxide gas pressure of beverages is 5.0kg/cm 2 , 4.5kg/cm 2 , or 4.0kg/cm 2 , and the lower limit is 0.20kg/cm 2 , 0.50kg/cm 2 , or 1.0kg/cm 2 2 , and any of these upper and lower limits can also be combined. For example, the carbon dioxide gas pressure of the beverage can also be 0.20kg/ cm2 to 5.0kg/ cm2 , 0.50kg/ cm2 to 4.5kg/ cm2 , or 1.0kg/ cm2 to 4.0kg/ cm2 . In this specification, the gas pressure means the gas pressure in the container except for special conditions. The pressure can be measured by fixing the sample at 20°C on the gas internal pressure gauge, first opening the valve of the gas internal pressure gauge to release the gas, then closing the valve, shaking the gas internal pressure gauge, and reading the time when the pointer reaches a certain position value, or use a commercially available gas pressure measuring device to measure.

本發明之無酒精啤酒風味飲料中,因應必要亦可含有各種添加物。 作為如此之添加物,有舉例如著色料、泡形成劑、發酵促進劑、酵母萃取物、含胜肽物等之蛋白質系物質、胺基酸(甘胺酸、脯胺酸等)等之調味料。 The non-alcoholic beer-flavored beverage of the present invention may contain various additives as necessary. Examples of such additives include colorants, foam forming agents, fermentation accelerators, yeast extracts, protein-based substances such as peptides, and seasonings such as amino acids (glycine, proline, etc.) material.

著色料為使用來對飲料賦予如啤酒之顏色,能夠使用焦糖色素等。As the coloring material, caramel coloring and the like can be used for coloring beverages such as beer.

泡形成劑為使用於在飲料中形成如啤酒之泡沫,或保持飲料之泡沫,能夠適當地使用大豆皂素、皂皮樹皂素等之植物萃取皂素系物質、玉米、大豆等之植物蛋白質及膠原蛋白胜肽等之含胜肽物、酵母萃取物等。Foam forming agents are used to form foam in beverages such as beer, or to maintain foam in beverages. Plant-extracted saponin-based substances such as soybean saponin and saponin, and plant proteins such as corn and soybeans can be used appropriately. And collagen peptides, etc. containing peptides, yeast extract, etc.

發酵促進劑為使用來促進酵母之發酵,能夠單獨或組合地使用例如酵母萃取物、米或麥等之糠成分、維他命、礦物質劑等。Fermentation accelerators are used to accelerate the fermentation of yeast, and for example, yeast extracts, bran components such as rice or wheat, vitamins, and mineral agents can be used alone or in combination.

本發明之無酒精啤酒風味飲料除了具有含有乙酸使其成為90~320質量ppm之步驟,並將pH值設為2.8~4.2之範圍內以外,能夠與任一種一般的無酒精啤酒風味飲料同樣地來製造。以下,作為一般的無酒精啤酒風味飲料之製造方法,有表示將麥芽作為原料使用與不使用時之製造方法。The non-alcoholic beer-flavored beverage of the present invention can be used in the same manner as any general non-alcoholic beer-flavored beverage, except that it has a step of containing acetic acid to 90 to 320 mass ppm, and sets the pH within the range of 2.8 to 4.2. to manufacture. Hereinafter, as a production method of a general non-alcoholic beer-flavored beverage, there are shown production methods when malt is used as a raw material and when it is not used.

將麥芽作為原料使用所製造之無酒精啤酒風味飲料,首先,在包含麥芽等之麥之外,因應必要亦包含其他穀物、澱粉、糖類、苦味料或著色料等之原料及水之混合物中,因應必要添加澱粉酶等之酵素,進行糊化、糖化,過濾後作為糖化液。因應必要,將啤酒花或苦味料等添加於糖化液並煮沸,於清澄槽中,去除凝固蛋白質等之固形分。作為此糖化液之代替,亦可於麥芽萃取物中有添加溫水者中添加啤酒花並煮沸。啤酒花亦可在自煮沸開始至煮沸結束前之任何階段來混合。糖化步驟、煮沸步驟、固形分除去步驟等中之條件使用熟知條件即可。煮沸後,進行冷卻,對所得之麥汁添加香料、酸味料、焦糖色素等之色素、抗氧化劑、苦味料、甜味料、胺基酸原料等,過濾後,於所得之過濾液中添加碳酸氣體。之後,填充於容器,並經過殺菌步驟,得到目的之無酒精啤酒風味飲料。作為本發明之製造方法中之步驟,在添加乙酸之型態中,在填充於容器之前之過濾前來添加較佳。Non-alcoholic beer-flavored beverages manufactured using malt as a raw material. First, in addition to malt, etc., other raw materials such as grains, starch, sugars, bittering materials or coloring materials and water are also mixed as necessary In the process, enzymes such as amylase are added as necessary to perform gelatinization and saccharification, and after filtration, it is used as a saccharification liquid. If necessary, add hops or bittering materials to the saccharification liquid and boil it, and remove the solid matter such as coagulated protein in the clarification tank. Instead of this saccharification solution, hops can also be added to the malt extract with warm water and boiled. Hops can also be mixed at any stage from the start of the boil to the end of the boil. The conditions in the saccharification step, the boiling step, the solid content removal step, etc. may use well-known conditions. After boiling, cool it, add spices, sour ingredients, caramel colorings, antioxidants, bittering ingredients, sweeteners, amino acid raw materials, etc. to the obtained wort, filter, and add to the obtained filtrate carbon dioxide gas. Afterwards, it is filled in a container and undergoes a sterilization step to obtain the target non-alcoholic beer-flavored beverage. As a step in the production method of the present invention, in the form of adding acetic acid, it is preferable to add it before the filtration before filling in the container.

不將麥芽作為原料使用所製造之無酒精啤酒風味飲料時,首先,將含有碳素源之液糖、麥或麥芽以外之作為含胺基酸之材料之氮源、啤酒花、色素等與溫水一起混合,作為液糖溶液。將該液糖溶液煮沸。使用啤酒花作為原料時,啤酒花亦可不用在煮沸開始前,而是在煮沸中混合至該液糖溶液。煮沸後,冷卻後,於所得之麥汁中添加香料、酸味料、焦糖色素等之色素、抗氧化劑、苦味料、甜味料、胺基酸原料等,過濾後,對所得之液糖溶液添加碳酸氣體。之後,填充於容器,經過殺菌步驟,得到目的之無酒精啤酒風味飲料。作為本發明之製造方法中之步驟,於添加乙酸之型態中,在填充於容器之前之過濾前來添加較佳。When non-alcoholic beer-flavored beverages are manufactured without using malt as a raw material, first, nitrogen sources, hops, pigments, etc. Mix together warm water for a liquid sugar solution. Bring the liquid sugar solution to a boil. When using hops as a raw material, the hops can also be mixed into the liquid sugar solution during boiling instead of starting before boiling. After boiling and cooling, add spices, sour ingredients, caramel pigments, antioxidants, bittering ingredients, sweeteners, amino acid raw materials, etc. to the obtained wort, and filter the obtained liquid sugar solution Add carbon dioxide gas. Afterwards, it is filled in a container and undergoes a sterilization step to obtain the target non-alcoholic beer-flavored beverage. As a step in the production method of the present invention, in the form of adding acetic acid, it is preferable to add it before the filtration before filling the container.

且,本發明之製造方法所得之無酒精啤酒風味飲料之收斂感及刺激感較優異。因此,本發明是提供一種關於賦予無酒精啤酒風味飲料之收斂感及刺激感之香味提升方法。且,本發明之香味提升方法之步驟之詳細與本發明之製造方法相同。 [實施例] Moreover, the non-alcoholic beer-flavored beverage obtained by the production method of the present invention has excellent astringent and stimulating sensations. Therefore, the present invention provides a method for enhancing the flavor of non-alcoholic beer-flavored beverages with astringent and stimulating sensations. And, the details of the steps of the method for improving the flavor of the present invention are the same as those of the production method of the present invention. [Example]

以下,以實施例具體地說明本發明,但本發明並不限制於下述實施例。Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited to a following Example.

<無酒精啤酒風味飲料之調製> 實施例1~11、比較例1~6 將水及麥芽放入準備槽中,調製約45℃之糊狀物,將此糊狀物快速地升溫至75~85℃之範圍後,以升溫後之特定之溫度,保持一定時間並攪拌後,移至麥汁過濾槽,進行過濾,得到麥汁。於所得之麥汁中添加溫水並稀釋,放入啤酒花、乳酸、水飴,使用煮沸釜,以100℃進行煮沸處理。煮沸處理後,移送至旋渦槽,將麥汁殘渣去除,冷卻至約1~6℃,得到冷卻液。添加酵母,進行發酵,調整成表1~2所記載之酒精濃度。添加合成乙酸使其成為表1~2中記載之濃度,進行過濾步驟,添加啤酒香料,實施碳酸氣體添加,實施瓶裝,得到無酒精啤酒風味飲料。 <Preparation of non-alcoholic beer-flavored drinks> Embodiment 1~11, comparative example 1~6 Put water and malt into the preparation tank, prepare a paste at about 45°C, quickly raise the temperature of the paste to the range of 75~85°C, and keep it at a specific temperature after heating for a certain period of time and stir Afterwards, move to the wort lauter tank and filter to obtain wort. Add warm water to the obtained wort and dilute it, add hops, lactic acid, and starch syrup, and boil it at 100°C in a boiling pot. After boiling, transfer to the vortex tank, remove wort residue, cool to about 1-6°C, and obtain cooling liquid. Add yeast, carry out fermentation, and adjust to the alcohol concentration recorded in Table 1~2. Add synthetic acetic acid to make it the concentration recorded in Tables 1 to 2, perform a filtration step, add beer fragrance, add carbon dioxide gas, and bottle it to obtain a non-alcoholic beer-flavored beverage.

<收斂感> 將各實施例・比較例之無酒精啤酒風味飲料冷卻至4℃後,關於「收斂感」,準備2杯飲料,統一評價基準。分數化以0.5之刻度來實施,算出5位官能檢查員之分數平均值。將結果表示於表1~2。 (收斂感之評價基準) 「1」:不適合 「2」:合格 「3」:較佳 <Sense of Convergence> After cooling the non-alcoholic beer-flavored drinks of each Example and Comparative Example to 4°C, regarding the "sense of astringency", 2 cups of drinks were prepared, and the evaluation criteria were unified. Scores are implemented on a scale of 0.5, and the average score of five sensory inspectors is calculated. The results are shown in Tables 1-2. (Evaluation criteria for sense of convergence) "1": not suitable "2": qualified "3": Better

<刺激感> 將各實施例・比較例之無酒精啤酒風味飲料冷卻至4℃後,關於「刺激感」,準備2杯飲料,統一評價基準。分數化以0.5之刻度來實施,算出5位官能檢查員之分數平均值。將結果表示於表1~2。 (刺激感之評價基準) 「1」:不適合 「2」:合格 「3」:較佳 <Stimulation> After cooling the non-alcoholic beer-flavored drinks of each Example and Comparative Example to 4°C, regarding the "stimulation", 2 cups of drinks were prepared, and the evaluation criteria were unified. Scores are implemented on a scale of 0.5, and the average score of five sensory inspectors is calculated. The results are shown in Tables 1-2. (evaluation criteria for stimulation) "1": not suitable "2": qualified "3": Better

<酸臭味> 將各實施例・比較例之無酒精啤酒風味飲料冷卻至4℃後,關於「酸臭味」,準備2杯飲料,統一評價基準。分數化以0.5之刻度來實施,算出5位官能檢查員之分數平均值。將結果表示於表1~2。 (酸臭味之評價基準) 「1」:不適合 「2」:合格 「3」:較佳 <Sour smell> After cooling the non-alcoholic beer-flavored drinks of each Example and Comparative Example to 4°C, regarding the "sour smell", 2 cups of drinks were prepared, and the evaluation criteria were unified. Scores are implemented on a scale of 0.5, and the average score of five sensory inspectors is calculated. The results are shown in Tables 1-2. (Evaluation criteria for sour odor) "1": not suitable "2": qualified "3": better

如表1所示,乙酸之含量為90~320質量ppm,pH值為2.8~4.2之實施例1~11之收斂感、刺激感皆優異,且能夠抑制酸臭味。As shown in Table 1, Examples 1 to 11 having an acetic acid content of 90 to 320 mass ppm and a pH value of 2.8 to 4.2 have excellent astringent and stimulating sensations, and can suppress sour odor.

<釀造醋之調製> 使用大麥麥芽,進行準備,將所得之麥汁以酵母進行酒精發酵。去除酵母後,添加食醋、乙酸菌,進行乙酸發酵,得到釀造醋。且,使用乙酸鈉,調整鈉含量,調製表3所示之釀造醋A~C。 <Preparation of brewed vinegar> Barley malt is used for preparation, and the resulting wort is subjected to alcoholic fermentation with yeast. After the yeast is removed, vinegar and acetic acid bacteria are added to carry out acetic acid fermentation to obtain brewed vinegar. And, using sodium acetate, adjusting the sodium content, prepared the brewed vinegars A~C shown in Table 3.

實施例12~24 除了添加上述調製之釀造醋來取代合成乙酸,並設為表4所示之組成以外,其餘與實施例1同樣地來調製無酒精啤酒風味飲料。關於收斂感、刺激感、酸臭味,進行與實施例1相同之評價。將結果表示於表4。 Example 12~24 A non-alcoholic beer-flavored drink was prepared in the same manner as in Example 1, except that the brewed vinegar prepared above was added instead of synthetic acetic acid, and the composition shown in Table 4 was used. The same evaluation as in Example 1 was performed about the astringent feeling, a stimulating feeling, and a sour smell. The results are shown in Table 4.

如表4所示可得知,使用釀造醋取代合成乙酸之實施例12~24中,相較於使用合成乙酸,酸臭味之抑制有較優異之傾向。As shown in Table 4, it can be seen that in Examples 12 to 24 in which brewed vinegar was used instead of synthetic acetic acid, the suppression of sour odor tended to be superior compared to the use of synthetic acetic acid.

<膨脹度> 將實施例1、12、17、18、19、21、24之無酒精啤酒風味飲料冷卻至4℃後,關於「膨脹度」,準備2杯飲料,統一評價基準。分數化以0.5之刻度來實施,算出5位官能檢查員之分數平均值。將結果表示於表5。 (膨脹度之評價基準) 「1」:不適合 「2」:合格 「3」:較佳 <Expansion> After cooling the non-alcoholic beer-flavored beverages of Examples 1, 12, 17, 18, 19, 21, and 24 to 4°C, 2 cups of beverages were prepared for the "overrun" and the evaluation criteria were unified. Scores are implemented on a scale of 0.5, and the average score of five sensory inspectors is calculated. The results are shown in Table 5. (Evaluation criteria for expansion degree) "1": not suitable "2": qualified "3": better

如表5所示可得知使用釀造醋取代合成乙酸之實施例12、17、18、19、21、24相較於使用合成乙酸時,膨脹度有較優異之傾向。As shown in Table 5, it can be known that Examples 12, 17, 18, 19, 21, and 24, which use brewed vinegar instead of synthetic acetic acid, have a tendency to have better expansion than when synthetic acetic acid is used.

實施例25~28 對市售之無酒精啤酒風味飲料(參考例1、2)添加合成乙酸或釀造醋,使其成為表6之乙酸量,調製無酒精啤酒風味飲料。與上述同樣地,關於收斂感、刺激感、酸臭味、膨脹度進行評價。將結果表示於表6。 Example 25~28 Add synthetic acetic acid or brewed vinegar to commercially available non-alcoholic beer-flavored beverages (Reference Examples 1 and 2) to make the amount of acetic acid shown in Table 6 to prepare non-alcoholic beer-flavored beverages. In the same manner as above, evaluations were performed about astringent feeling, irritating feeling, sour smell, and degree of swelling. The results are shown in Table 6.

如表6所示可得知,即使於市售品中添加乙酸時,收斂感、刺激感較優異,且亦能夠抑制酸臭味。尤其是,添加釀造醋之實施例26、28中,酸臭味之抑制較優異,且關於膨脹度也較優異。 [產業利用性] As shown in Table 6, even when acetic acid is added to a commercially available product, it is found that the astringent feeling and stimulating feeling are excellent, and the sour smell can also be suppressed. In particular, in Examples 26 and 28 in which brewed vinegar was added, the suppression of sour odor was excellent, and it was also excellent in the swelling degree. [Industrial Utilization]

藉由本發明,能夠提供一種收斂感、刺激感優異之新穎之風味之無酒精啤酒風味飲料。According to the present invention, it is possible to provide a non-alcoholic beer-flavored drink with a novel flavor that is excellent in astringency and stimulation.

Claims (10)

一種無酒精啤酒風味飲料,其中,乙酸之含量為90~320質量ppm,pH值為2.8~4.2。A non-alcoholic beer-flavored beverage, wherein the content of acetic acid is 90-320 mass ppm, and the pH value is 2.8-4.2. 如請求項1之無酒精啤酒風味飲料,其中,包含釀造醋。The non-alcoholic beer-flavored beverage as in claim 1, which contains brewed vinegar. 如請求項2之無酒精啤酒風味飲料,其中,包含經添加之釀造醋。The non-alcoholic beer-flavored beverage as in claim 2, which includes added brewed vinegar. 如請求項2或3之無酒精啤酒風味飲料,其中,前述釀造醋中之鈉含量為100~1000質量ppm。The non-alcoholic beer-flavored beverage according to claim 2 or 3, wherein the sodium content in the brewed vinegar is 100-1000 ppm by mass. 如請求項1~4中任一項之無酒精啤酒風味飲料,其中,乙酸乙酯之含量為0.1~60.0質量ppm。The non-alcoholic beer-flavored beverage according to any one of claims 1 to 4, wherein the content of ethyl acetate is 0.1 to 60.0 ppm by mass. 如請求項1~5中任一項之無酒精啤酒風味飲料,其中,己酸乙酯之含量為1.0~900.0質量ppb。The non-alcoholic beer-flavored beverage according to any one of claims 1 to 5, wherein the content of ethyl caproate is 1.0 to 900.0 ppb by mass. 一種pH值為2.8~4.2之無酒精啤酒風味飲料之製造方法,其中,包含使乙酸成為90~320質量ppm來含有之步驟。A method for producing a non-alcoholic beer-flavored beverage having a pH of 2.8 to 4.2, including the step of containing acetic acid at 90 to 320 ppm by mass. 如請求項7之製造方法,其中,包含含有釀造醋之步驟。The manufacturing method as claimed in item 7, which includes the step of brewing vinegar. 如請求項8之製造方法,其中,前述釀造醋中之鈉含量為100~1000質量ppm。The manufacturing method according to claim 8, wherein the sodium content in the aforementioned brewed vinegar is 100-1000 mass ppm. 一種對pH值為2.8~4.2之無酒精啤酒風味飲料賦予收斂感或刺激感之方法,其中,包含使乙酸成為90~320質量ppm來含有之步驟。A method for imparting an astringent or stimulating feeling to a non-alcoholic beer-flavored beverage having a pH of 2.8 to 4.2, comprising a step of containing acetic acid at 90 to 320 mass ppm.
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