TW202322784A - Skin caring method containing liposome covering the T4 endonuclease V - Google Patents

Abstract

This invention relates to a composition containing T4 endonuclease V. The composition contains liposome covering the T4 endonuclease V. This invention additionally provides a skin caring method including the following steps: cleaning the face of a user; applying the composition containing the T4 endonuclease V on the face; laying a 3D dry hyaluronic acid mask and an essence mask on the face in sequence, and massaging the face; and finally, applying a cream or lotion on the face, and massaging the face. By means of the applying and massaging steps in the skin caring method provided by this invention, the cream or the lotion is more easily absorbed by skin cells of the face, then, a better DNA restoration effect is achieved, the inherent skin elasticity of the skin cells of the face is restored, and thus, generation of fine wrinkles is reduced.

Description

Composition containing T4 endonuclease V and cosmetic method for skin

The present invention relates to a composition containing T4 endonuclease V and a cosmetic method for the skin, in particular to a composition using T4 endonuclease V with the function of repairing DNA and a cosmetic method for promoting skin absorption of the composition method.

There are many reasons for the aging of the facial skin, and one of the culprits is the ultraviolet rays in the environment. In daily life, people often only pay attention to the sun protection work under the sun, and ignore even if they are exposed to the sun for a short time outdoors. Or under the illumination of indoor lighting equipment, even when using an electric heater with a halogen lamp tube, the above situation will still expose people to a trace of ultraviolet rays, and the long-term accumulation of traces of ultraviolet rays will cause skin cells to Damaged DNA may affect the slowdown of skin cell renewal or reduce the ability to repair DNA, eventually leading to accelerated skin aging, sagging, wrinkles or loss of elasticity, etc., making people look much older than their actual age.

Therefore, various facial cosmetics are also produced thereupon, and the aging skin of facial parts can be covered quickly through the smearing of cosmetics, making people's facial skin look better, but the cosmetics used are still only temporary Sexual concealer is not a long-term solution.

In order to solve various skin problems caused by the DNA in the skin cells of the face being affected by ultraviolet rays in the past, the present invention provides a composition containing T4 endonuclease V, which has the function of repairing the DNA of the above-mentioned skin cells affected by ultraviolet rays , so that the cells can return to normal operation, and then restore the skin to a normal healthy state and inherent skin elasticity, so as to reduce the appearance of fine lines.

The main purpose of the present invention is to provide a composition containing T4 endonuclease V, comprising: a liposome, which coats a T4 endonuclease V (T4 endonuclease V).

In one embodiment of the present invention, the microlipid system is surrounded by a double layer of phospholipids.

In one embodiment of the present invention, the microlipid system is selected from lipid microliposome (Liposome), phosphatidylcholine (Phosphatidylcholine), phospholipid microliposome (Phytosome), phospholipid serine (Phosphatidylserine), ethanol microlipid A group consisting of Ethosome, Phosphatidylinositol and their combinations.

In one embodiment of the present invention, the liposome has a particle size of 100 nm to 5000 nm.

In one embodiment of the present invention, the concentration of the T4 endonuclease V is less than 1.0 mg and greater than or equal to 0.0001 mg per milliliter, or less than 1.0 mg and greater than or equal to 0.0001 mg per milligram. The coverage rate is less than or equal to 80%.

Based on the main purpose, the present invention additionally provides a skin beautifying method, which includes the following steps: (a) using a composition containing T4 endonuclease V and applying it to a face; (b) covering with a 3D dry hyaluronic acid mask on the face.

In one embodiment of the present invention, the 3D dry hyaluronic acid facial mask has a hyaluronic acid crystal column (30).

Based on the main purpose, the present invention additionally provides a skin beautifying method, which comprises the following steps: (a) cleaning a user's face; (b) using a composition containing T4 endonuclease V and applying it on the face (c) cover the face with a 3D dry hyaluronic acid mask; (d) massage the face covered with the 3D dry hyaluronic acid mask by a massage method; (e) apply an essence mask (4) On the 3D dry hyaluronic acid mask; massage the face covered with the essence mask through the massage method; (g) During the massage process in the step, add a mask essence to the essence mask and then massage (h) remove the essence mask and the 3D dry hyaluronic acid mask;

In one embodiment of the present invention, after the step (h), a step (i) is further included, which is to repeat the step (b) and slap the face.

In one embodiment of the present invention, after the step (i), a step (j) is further included, applying a cream or a lotion on the face.

In one embodiment of the present invention, after the step (j), a step (k) is further included to massage the face through the massage method.

In one embodiment of the present invention, the face is a whole face part, a forehead part, a part around the eyes, a cheek part, a part around the mouth or a part under the chin.

In one embodiment of the present invention, the part around the eyes is any one of a first part around the eyes, a second part around the eyes or a third part around the eyes, or any two or three of them.

In one embodiment of the present invention, the massage method uses the pads of fingers.

In one embodiment of the present invention, the massage method uses a temperature induction device, an ultrasound induction device or an electric charge induction device.

In one embodiment of the present invention, the massage method uses a massage tool with a protruding part.

In one embodiment of the present invention, the essence mask is made of gel, biological fiber, plant fiber, non-woven fabric, silk, pure cotton cloth or paper pulp.

In one embodiment of the present invention, the cream or the emulsion system further comprises a liposome, and the liposome is coated with a whitening substance, and the whitening substance is selected from coenzyme Q10 (coenzyme Q10), vitamin B12, vitamin C, The group consisting of kojic acid, arbutin, tranexamic acid, kojic acid, ellagic acid, chamomile ET and combinations thereof .

In this way, in addition to using the composition of T4 endonuclease V, which has the function of repairing DNA, the present invention can also be used with a 3D dry hyaluronic acid mask. Through the steps of smearing and massaging, the skin cells on the face can more easily absorb the T4 The composition of endonuclease V and mask essence exert a better effect of repairing skin cell DNA, allowing facial skin cells to restore the inherent skin elasticity and reduce the appearance of fine lines.

In order to facilitate the understanding of the technical features, content and advantages of the present invention and the effects it can achieve, the present invention is described in detail below in conjunction with the accompanying drawings, and the drawings used therein are only intended to illustrate and assist instructions The purpose of the invention is not necessarily the true proportion and precise configuration after the implementation of the present invention, so the proportion and configuration relationship of the attached drawings should not be interpreted or limited to the scope of rights of the present invention in actual implementation.

In order to make the description of the disclosed content of the present invention more detailed and complete, the following provides an illustrative description of the implementation aspects and specific embodiments of the present invention; but this is not the only form for implementing or using the specific embodiments of the present invention.

In the whole of this specification, the expression of a singular form should be understood as including the concept of the plural form as long as there is no particular mention. In addition, the terms used in the present specification should be understood to be used in the meanings commonly used in the relevant field unless otherwise mentioned. Therefore, unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as generally understood by those skilled in the art to which the present invention belongs.

The definitions and/or basic technical contents of the terms used in this specification are appropriately described below.

The liposome mentioned in the present invention refers to an artificially prepared and/or naturally prepared vesicle, which is composed of at least one bilayer phospholipid layer structure and its combination. A liposome can be a unilamellar vesicle (i.e. a vesicle consisting of a single bilayer phospholipid layer structure and its combination) or a multilamellar vesicle (i.e. a vesicle consisting of multiple bilayer phospholipid layer structures and combinations thereof) vesicles).

The T4 endonuclease V (T4 endonuclease V, referred to as T4N5) mentioned in the present invention is a DNA repair enzyme in T4 bacteriophage, and its size is a protein of 16 kDa. T4 endonuclease V can specifically identify the The cyclobutane pyrimidine dimer (CPD) formed after the DNA is affected by ultraviolet light is excised and then repaired by the subsequent DNA repair mechanism. In this case, the T4 endonuclease V series can be an endonuclease transcribed and translated with a similar or identical DNA sequence. The above-mentioned similar or identical DNA sequence can also be produced by gene recombination or chemical modification, and the above-mentioned T4 Endonuclease V is not limited to be produced by a specific species, moreover, the T4 endonuclease V mentioned in the present invention is not limited to this name, but can include any one with T4 endonuclease V function The name.

The 3D dry hyaluronic acid mask mentioned in the present invention has a three-dimensional hyaluronic acid crystal column. The 3D dry hyaluronic acid mask is made of 20~400 micron ( μm) of 3D hyaluronic acid structure, the above 3D dry hyaluronic acid system uses middle molecular hyaluronic acid as raw material, and is made from middle molecular hyaluronic acid itself at -30°C~150°C. Hyaluronic acid is a linear glycosaminoglycan composed of D-glucuronic acid and N-acetyl glucosamine basic structure. The 3D dry hyaluronic acid mask with three-dimensional hyaluronic acid crystal pillars is prepared in compliance with the US FDA's guidelines for microneedle products (please refer to the US FDA (Food and Drug Administration, Food and Drug Administration) on November 2020 The document (Guidance for Industry and Food and Drug Administration Staff) issued on March 10), and in the chapter of microneedle products in the above document, it can be understood that the microneedle patch is not a medical device, and it is in the Federal Food, Drug and Drug Administration Staff Subsection 201(h) of the Cosmetic Bureau Act (FD&C Act) has the effect of promoting skin exfoliation (that is, destroying the stratum corneum formed by dead skin cells) or improving the appearance of skin without penetrating living skin cells. Acupuncture products are not devices for diagnosing or treating diseases.

The main purpose of the present invention is to provide a composition containing T4 endonuclease V, comprising: a liposome, the liposome is coated with a T4 endonuclease V (T4 endonuclease V, referred to as T4N5), the The particle size of the liposome is 100 nanometers (nm) ~ 5000 nanometers (nm), and the T4 endonuclease V concentration is approximately less than 1.0 milligrams (mg) and greater than or equal to 0.0001 milligrams (mg) per milliliter or Each milligram has less than 1.0 milligram (mg) and greater than or equal to 0.0001 milligram (mg). Since T4 endonuclease V is a hydrophilic macromolecule, it is difficult to penetrate the stratum corneum of the skin without being coated in a carrier. Therefore, the present invention purposely selects the particle size range value of specific liposomes to achieve Satisfied that the T4 endonuclease V can be coated at a concentration of less than 1.0 mg and greater than or equal to 0.0001 mg per milliliter, or less than 1.0 mg and greater than or equal to 0.0001 mg per milligram, and the encapsulation of the liposome Under the condition that the coverage rate is less than or equal to 80%, the composition can pass through the stratum corneum barrier of the skin, and then penetrate into the dermis to repair the DNA of skin cells affected by ultraviolet rays. In actual implementation, when the user (U) applies the film composition on the face, the usage amount can be less than 5 ml each time, but it can also be used according to the actual needs of the user (U).

In one embodiment of the present invention, the composition containing T4 endonuclease V is an emulsion prepared by coating T4 endonuclease V with liposomes. The T4 endonuclease V can be stored at room temperature through freeze-drying and vacuum packaging techniques, and can retain its enzyme activity when used after redissolving. The composition containing T4 endonuclease V can be used to prepare liposomes containing T4 endonuclease V by alcohol injection method or film/ultrasonic vibration method.

The microlipid system is formed surrounded by double-layer phospholipid layer; the microlipid system is selected from lipid liposome (Liposome), phosphatidylcholine (Phosphatidylcholine), phospholipid liposome (Phytosome), phospholipid serine ( A group consisting of Phosphatidylserine), Ethosome, Phosphatidylinositol and combinations thereof. The microlipid system has good cell membrane permeability, not only can be absorbed and decomposed by skin cells, but also has no protein immune rejection and non-toxic characteristics, so as to reduce adverse allergic reactions on the face. In actual implementation, this The inventive composition can be used safely by users (U) with sensitive skin by being coated in liposomes. Furthermore, liposomes can also control the release rate of the encapsulated T4 endonuclease V and prolong its half-life, so that the T4 endonuclease V can maintain a certain activity and be used to repair the cells affected by ultraviolet rays. The DNA of skin cells allows the cells to resume normal operation, thereby restoring the skin to a normal healthy state and inherent skin elasticity to reduce the appearance of fine lines.

Please refer to Figures 1 to 5, the present invention provides a skin cosmetic method (1) based on the main purpose, which includes the following steps: (a) using a composition containing T4 endonuclease V and applying In one face (F), since T4 endonuclease V is a hydrophilic macromolecule, it is difficult to penetrate the stratum corneum of the skin without being coated in a carrier, so the present invention selects a specific carrier purposefully through the above-mentioned ( liposome), so that the liposome coated with T4 endonuclease V can pass through the stratum corneum barrier of the skin, and then penetrate into the dermis to repair the DNA of skin cells affected by ultraviolet rays; (b) A 3D dry hyaluronic acid mask (3) covers the face (F). The 3D dry hyaluronic acid mask (3) has a hyaluronic acid crystal column (30). The hyaluronic acid crystal column (30) has a certain mechanical strength and can be used for Destroy the stratum corneum, so that the face covered by the 3D dry hyaluronic acid mask can more easily absorb the composition containing T4 endonuclease V.

The present invention can use the composition containing T4 endonuclease V to repair the DNA of skin cells affected by ultraviolet rays, so that the cells can return to normal operation and restore the youthful state of the skin to maintain skin elasticity. At the same time, The moisture-locking and moisturizing effect of the 3D dry hyaluronic acid mask (3) not only reduces the loss of skin moisture, but also keeps the facial skin supple.

The 3D dry hyaluronic acid mask system can be exemplified but not limited to include water, sodium hyaluronate (sodium hyaluronate), cyclodextrin (cyclodextrin), lecithin emulsifier (sodium acrylates/acrolein copolymer), pullulan (amylopectin), Ubiquinone, sodium acrylate/acrylonitrogens copolymer, lecithin, polysorbate-20, glycerin, Rice bran wax, fructo-oligosaccharides, calcium pyrophosphate, L-Ascorbic Acid 2-Glucoside or ascorbyl glucoside at least One or a combination of two or more. The middle molecular hyaluronic acid contained in this 3D dry hyaluronic acid has the ability to moisturize the skin.

Based on the main purpose, the present invention additionally provides a skin beautifying method (1), which includes step (a) to step (k), and will be described according to the following steps:

Please refer to Figure 2 first, step (a) cleans a face of a user (U), the user (U) can use facial cleansing products to clean the face, or only use light water or warm water to clean the face dirt.

Step (b) Use a composition containing T4 endonuclease V and apply it on the face (F). In actual practice, the user (U) can use about 1 ml of the composition and apply it evenly on the The face (F).

Please refer to Figure 3 again. Step (c) covers the face with a 3D dry hyaluronic acid mask (F). The hyaluronic acid mask can be used to moisturize the skin. Please also refer to Figure 6, and The 3D dry hyaluronic acid mask system has a hyaluronic acid crystal column (30). The hyaluronic acid crystal column (30) has a certain mechanical strength and can be used to destroy the cuticle, so that the face (F) covered by the 3D dry hyaluronic acid mask can be better. Easily absorbed compositions containing T4 endonuclease V.

Step (d) Massage the face (F) covering the 3D dry hyaluronic acid mask through a massage method. The massage method is to use the fingertips to gently press the 3D dry hyaluronic acid mask back and forth. In actual implementation, the user (U) Gently press back and forth on the face covering the 3D dry hyaluronic acid mask through the pulp of the fingers, and the gentle pressure can be 3 to 5 minutes at a time; instrument; this massage method uses a massage tool with a protruding piece, and the protruding piece can be in any shape or arrangement that is conducive to facial massage. Raised pieces.

Please refer to Fig. 4 again, in step (e) apply an essence mask (4) on the 3D dry hyaluronic acid mask, the essence mask (4) is made of gel, biological fiber, plant fiber , non-woven fabric, silk, pure cotton or pulp.

Step (f) Massage the face (F) covered with the essence mask (4) through the massage method, so that the hyaluronic acid crystal column (30) can be dissolved in the massage. The massage method is to use the pulp of the fingers, For example, the finger pulp of the ring finger can be used to gently press back and forth on the face covering the essence mask (4), and the gentle pressure can be 3 minutes to 5 minutes at a time, or a temperature induction instrument, an ultrasound introduction instrument, an electric charge introduction instrument or A massage tool with a protruding part. The essence mask (4) can be exemplified but not limited to include water, sodium hyaluronate and polysorbate-20.

Step (g) During the massage process of step (f), add a mask essence to the essence mask (4) and then massage. When massaging, slowly add mask essence to the essence mask (4) ), in addition to allowing the 3D dry hyaluronic acid to slowly dissolve and moisturize the skin, it also allows the facial skin to fully contact and absorb the mask essence.

The mask essence system can be exemplified but not limited to water, sodium hyaluronate (sodium hyaluronate), propylene glycol, glycerin, butylene glycol, panthenol, isostearic acid, PEG-40 hydrogenated castor oil, cyclopentamer Cyclopentasiloxane, phenoxyethanol, isononyl isononanoate, ammonium polyacryloyldimethyl taurate, chlorphenesin ), hydroxypropyl guar, ginkgo biloba leaf extract, undaria pinnatifida extract, capric/caprylic triglycerides, octylphenol polyether -10 (octoxynol-10), laureth-7 (laureth-7), polysorbate-20 (polysorbate-20), bisabolol (Alpha Bisabolol), dipotassium glycyrrhizate (dipotassium glycyrrhizate), Rose (ROSA SPP.) flower oil, allantoin, ethylhexylglycerin, escin, ruscus aculeatus root extract, ammonium glycyrrhizate , centella asiatica leaf extract, cyclotetrasiloxane, hydrolyzed yeast protein, calendula officinalis flower extract or caprylyl glycol at least one or a combination of two or more .

Step (h) removing the essence mask (4) and the 3D dry hyaluronic acid mask (3), and removing the used essence mask (4) and 3D dry hyaluronic acid mask (3).

Step (i) Repeat step (b) and pat the face (F). The user (U) can use about 1ml of the composition, apply the film evenly on the face (F), and pat the face Make the blood circulation of the face smooth, so as to facilitate the skin cells to absorb the ingredients.

Please refer to Figure 5 again, step (j) apply a cream or a lotion on the face (F), the user (U) can evenly apply the cream or lotion on the face (F) , the cream or the emulsion system further comprises a liposome, the liposome is coated with a whitening substance, and the whitening substance is selected from coenzyme Q10 (coenzyme Q10), vitamin B12, vitamin C, Kojic Acid (Kojic Acid) , arbutin, tranexamic acid, kojic acid, ellagic acid, chamomile ET and their combinations, the whitening substance can In order to have the effect of inhibiting melanocytes from producing melanin or lightening spots and whitening, the cream or the lotion itself has a moisturizing effect, and can seal the above-mentioned composition containing T4 endonuclease V, mask essence or whitening substances to make the skin Cells can continue to absorb.

Step (k) massage the face (F) through the massage method, and make the blood circulation of the face smooth, so as to facilitate the skin cells to absorb the composition containing T4 endonuclease V, mask essence or whitening substance.

In one embodiment of the present invention, the face (F) is a whole face, a forehead, the eye area (2), a cheek, a mouth area or a chin area, and the user (U) can follow the For actual needs, for example: when the skin of the face produces crow's feet, fine lines around the eyes, fine lines on the lower eyelids, fine lines around the eyes, jaw lines, eyebrow lines, lip lines, drooling lines, nasolabial folds or forehead lines, etc., The user (U) can select a suitable facial part for skin care.

In one embodiment of the present invention, the eye area (2) is any one of a first eye area (20), a second eye area (21) or a third eye area (22) or For any two or three, the user (U) can use the composition of the present invention or the skin cosmetic method (1) for skin care for different eye areas (2) according to actual needs.

Then, in order to enable the review committee to further understand the purpose, characteristics, and desired effects of the present invention, the analysis test of the skin cosmetic method mentioned in the present invention using the composition containing T4 endonuclease V is given below. , to prove that the cosmetic method of the present invention can promote skin absorption of the composition, and to further prove the scope of practical application of the present invention, but it is not intended to limit the scope of the present invention in any form.

The skin moisture content test, skin elasticity test and skin fairness test of the present invention are tested on the skin of the subject using a skin moisture content detector (Imate skin moisture detector, product model: M-6602).

The test result system of the present invention uses Excel software to carry out statistical analysis. The data are expressed as mean ± standard deviation (SD), and the differences between groups are analyzed by Student's t-test, and *** indicates p <0.001; ** indicates p <0.01; * indicates p <0.05.

<Skin elasticity test before and after maintenance with the composition of the present invention and 3D dry hyaluronic acid mask>

Test method: Each subject used the skin beauty method of the present invention on the day of the test. After cleaning the subject's face, he applied a composition containing T4 endonuclease V and covered with a 3D dry hyaluronic acid mask. At the same time, massage with fingertips to promote absorption for skin maintenance, and then use a skin analyzer to test the skin elasticity of the first eye area around the eyes. The pre-maintenance test is to test the first eye area after the subject cleans the face.

Test results: Please refer to Figure 7, which is a comparison chart of the skin elasticity results before and after applying the composition of the present invention and the 3D dry hyaluronic acid mask. The X-axis represents different groups before and after maintenance, and the Y-axis represents skin elasticity ( %). From the test results, it can be seen that the skin elasticity value after maintenance is significantly increased by about 32.0% compared with the skin elasticity before maintenance (* indicates p <0.05), which means that the skin beauty method of the present invention is helpful for recovery Skin elasticity.

<Skin moisture content and skin elasticity test before and after applying the composition of the present invention, 3D dry hyaluronic acid mask and cream>

Test method: Each subject used the skin beauty method of the present invention on the day of the test. After cleaning the subject's face, smear the composition containing T4 endonuclease V, cover the 3D dry hyaluronic acid mask and smear Cream, in which the fingertips are gently massaged to promote absorption for skin maintenance, and then use a skin analyzer to test the skin elasticity of the first eye area around the eyes. The pre-maintenance test is to test the first eye area after the subject cleans the face.

Test results: Please refer to Figure 8, which is a comparison chart of skin moisture content and skin elasticity before and after applying the composition of the present invention, 3D dry hyaluronic acid mask and cream, and the X-axis is the different groups before and after maintenance , and the Y-axis is skin elasticity (%) and skin moisture content (%) respectively. Compared with the test in Figure 7, this test has more cream applied to the facial skin. From the test results, it can be seen that the skin elasticity after maintenance is significantly increased by about 16.7% compared with the skin elasticity before maintenance (** indicates p <0.01). Moreover, the skin moisture content after maintenance is compared with The moisture content of the skin before maintenance, the value of the skin moisture content significantly increased by about 18.9% (* indicates p <0.05), which means that using the skin beauty method of the present invention can help restore skin elasticity and increase skin moisture content.

<Skin fairness test of the 0th week and the 2nd week of continuous application of the composition of the present invention, 3D dry hyaluronic acid mask and cream>

Test method: Twice a week, each subject uses the skin beauty method of the present invention on the test day, after cleaning the subject's face, apply a composition containing T4 endonuclease V, covering 3D Dry hyaluronic acid mask and apply cream. At the same time, massage with fingertips to promote absorption for skin maintenance. Then use a skin analyzer to test the skin elasticity of the first eye area around the eyes. The pre-maintenance test is to test the first eye area after the subject cleans the face.

Test results: Please refer to Figure 9, which is a comparison chart of the skin whiteness results of the 0th week and the 2nd week of continuous application of the composition of the present invention, 3D dry hyaluronic acid mask and cream, and the X-axis is the 0th week Compared with the group in the second week, the Y-axis is the skin fairness (%). From the test results, it can be seen that the fairness of the skin after maintenance is significantly increased by about 13.5% compared to the fairness of the skin before maintenance (* means p <0.05), which represents the long-term use of the skin beauty method of the present invention Helps improve skin fairness.

Please refer to Fig. 10 and Fig. 11, which are real photos (1) and (2) of unmaintained and maintained subjects applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. From the subject's face (F), it can be distinguished that the left face is maintained by using the skin care method of the present invention, while the right face is not maintained. For the nasolabial folds of the subject's face (F) (F1, F1'), it can be found that the nasolabial folds on the left face (F1) are compared with the nasolabial folds on the right face (F1'), on the side of the subject's face and the front face of the nasolabial folds on the left face The wrinkles (F1) are less obvious. Therefore, the skin cosmetic method of the present invention can smooth the nasolabial folds of the subject and make the subject look younger.

Please also refer to Figure 12, which is the actual photos taken before and after the subject applied the composition of the present invention, 3D dry hyaluronic acid mask and cream for maintenance. For the glabellar lines (F2) of the subject's face (F), the fine lines before and after maintenance were compared. Grow lines (F2') have obvious fine lines, however, the glabellar lines (F2) become less obvious after maintenance, therefore, the fine lines of the face can be smoothed through the skin beauty method of the present invention, allowing the subjects The skin has elasticity, and achieve the effect of reducing wrinkles.

Please refer to Fig. 13 and Fig. 14, which are real photos (1) and (2) of another subject applying the composition of the present invention, 3D dry hyaluronic acid mask and cream without maintenance and maintenance. From another subject's face (F), it can be distinguished that the right face is maintained by using the skin care method of the present invention, while the left face is not maintained. For the subject's face (F) For nasolabial folds (F1, F1'), it can be found that the nasolabial folds (F1) on the right face are compared with the nasolabial folds (F1') on the left face. The nasolabial folds (F1) of the subject are less obvious, therefore, the subject's nasolabial folds can be smoothed through the skin cosmetic method of the present invention, making the subject look younger.

In summary, compared with the prior art and products, the present invention has one of the following advantages:

One of the objectives of the present invention is to repair the DNA of skin cells affected by ultraviolet rays through the composition containing T4 endonuclease V, so that the cells can resume normal operation, and then restore the skin to a normal, healthy state and inherent skin Elasticity to reduce the appearance of fine lines.

One of the objectives of the present invention is to use liposomes as carriers to coat T4 endonuclease V through liposomes with hydrophilic and hydrophobic characteristics, and because liposomes themselves have better cell membrane penetration Sexuality, therefore, makes it easier for T4 endonuclease V to be taken up by skin cells.

One of the objectives of the present invention is to use the hyaluronic acid crystal column and massage method of the 3D dry hyaluronic acid mask to achieve the effect of exfoliating the facial skin, and to make the composition containing T4 endonuclease V more smoothly absorbed by the skin cells Absorb, and at the same time, through the water-locking and moisturizing effect of the 3D dry hyaluronic acid mask, to reduce the loss of skin moisture, and at the same time keep the facial skin supple.

One of the objectives of the present invention is to use the 3D dry hyaluronic acid mask, through the steps of smearing and massaging, the skin cells on the face can more easily absorb the composition containing T4 endonuclease V and the mask essence, and play better The effect of repairing the DNA of skin cells allows the skin cells of the face to restore the inherent skin elasticity and reduce the appearance of fine lines.

But what is described above is only an embodiment of the present invention, and should not limit the scope of the present invention. All simple equivalent changes and modifications made according to the patent scope of the present invention and the content of the patent specification are still within the scope of the present invention. Within the scope covered by the patent of the present invention.

[this invention] (1): skin beauty method (2): around the eyes (20): the first eye area (21): Second eye area (22): The third eye area (3): 3D dry hyaluronic acid mask (30): hyaluronic acid crystal column (4): Essence Mask (U): User (F): face (F1, F1’): Law lines (F2, F2'): Grow lines (a)~(k): steps

Figure 1: The overall flow chart of the cosmetic method for skin in a preferred embodiment of the present invention. Fig. 2: Schematic flow chart (1) of the cosmetic method for skin in one of the preferred embodiments of the present invention. Figure 3: Schematic flow chart (2) of the cosmetic method for skin in one of the preferred embodiments of the present invention. Figure 4: Schematic flow chart (3) of the cosmetic method for skin in one of the preferred embodiments of the present invention. Fig. 5: Schematic flow chart (four) of the cosmetic method for skin in one of the preferred embodiments of the present invention. Figure 6: A schematic diagram of a 3D dry hyaluronic acid mask according to a preferred embodiment of the present invention. Figure 7: It is a comparison chart of skin elasticity before and after applying the composition of the present invention and 3D dry hyaluronic acid mask. Figure 8: It is a comparison chart of water content and skin elasticity before and after applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. Figure 9: It is a comparison chart of the skin fairness results in the 0th week and the 2nd week of continuous application of the composition of the present invention, 3D dry hyaluronic acid mask and cream. Figure 10: It is a real photo (1) of unmaintained and maintained subjects applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. Figure 11: It is a real photo (2) of unmaintained and maintained subjects applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. Figure 12: It is a real photo of the test subject before and after applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. Fig. 13: It is a photo (1) of non-maintenance and maintenance of another subject applying the composition of the present invention, 3D dry hyaluronic acid mask and cream. Fig. 14: It is a real photo (2) of non-maintenance and maintenance of another subject applying the composition of the present invention, 3D dry hyaluronic acid mask and cream.

(a)~(k): steps

Claims (18)

A composition containing T4 endonuclease V, comprising: a liposome coated with a T4 endonuclease V (T4 endonuclease V). The composition containing T4 endonuclease V according to claim 1, wherein the microlipid system is surrounded by a double layer of phospholipids. The composition containing T4 endonuclease V as described in Claim 1, wherein the microlipid system is selected from lipid liposome (Liposome), phosphatidylcholine (Phosphatidylcholine), phospholipid liposome (Phytosome) , Phosphatidylserine (Phosphatidylserine), ethanol liposome (Ethosome), phosphatidylinositol (Phosphatidylinositol) and a group consisting of combinations thereof. The composition containing T4 endonuclease V according to any one of claims 1 to 3, wherein the particle size of the liposome is 100 nm to 5000 nm. The composition containing T4 endonuclease V as described in claim 4, wherein the concentration of the T4 endonuclease V is less than 1.0 mg and greater than or equal to 0.0001 mg per milliliter, or less than 1.0 mg per mg And greater than or equal to 0.0001 mg, the encapsulation rate of the liposome is less than or equal to 80%. A cosmetic method for the skin, comprising the following steps: (a) using a composition containing T4 endonuclease V as described in any one of claims 1 to 5 and applying it to a face (F); (b ) Cover the face (F) with a 3D dry hyaluronic acid mask (3). The cosmetic method for skin according to claim 6, wherein the 3D dry hyaluronic acid mask (3) has a hyaluronic acid crystal column (30). A cosmetic method for skin, comprising the following steps: (a) cleaning a face (F) of a user (U); (b) use a composition containing T4 endonuclease V as described in any one of claims 1 to 5 and apply it to the face (F); (c) Cover the face (F) with a 3D dry hyaluronic acid mask (3); (d) Massage the face (F) covering the 3D dry hyaluronic acid mask (3) by a massage method. (e) Paste an essence mask (4) on the 3D dry hyaluronic acid mask (3); (f) Massage the face (F) covered with the essence mask (4) through the massage method; (g) During the massage process of step (f), add a mask essence to the essence mask (4) and then massage; (h) Take off the essence mask (4) and the 3D dry hyaluronic acid mask (3); The cosmetic method for skin according to claim 8, wherein after step (h), further includes a step (i) of repeating step (b) and patting the face (F). The cosmetic method for skin according to claim 9, wherein after step (i), further includes a step (j), applying a cream or a lotion on the face (F). The cosmetic method for skin according to claim 10, further comprising a step (k) of massaging the face (F) through the massage method after the step (j). The cosmetic method for skin according to claim 8, wherein the face (F) is a whole face, a forehead, a part around the eyes (2), a cheek, a part around the mouth or a part under the chin. The cosmetic method for skin according to claim 12, wherein the eye area (2) is a first eye area (20), a second eye area (21) or a third eye area ( 22) Any one or any two or three. The skin beauty method according to any one of Claims 8 to 13, wherein the massage method uses the pads of fingers. The cosmetic method for skin according to any one of Claims 8 to 13, wherein the massage method uses a temperature induction device, an ultrasound induction device or an electric charge induction device. The skin beauty method according to any one of Claims 8 to 13, wherein the massage method uses a massage tool with a protrusion. The cosmetic method for skin according to claim 8, wherein the essence mask (4) is made of gel, biological fiber, plant fiber, non-woven fabric, silk, pure cotton cloth or paper pulp. The cosmetic method for skin as described in Claim 8, wherein the cream or the emulsion further comprises a liposome, and the liposome is coated with a whitening substance selected from coenzyme Q10 (coenzyme Q10) , vitamin B12, vitamin C, kojic acid, arbutin, tranexamic acid, kojic acid, ellagic acid, chamomile ET and The group formed by its combination.

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