TW202233258A - Method for treating medical waste container - Google Patents

Abstract

The present invention provides a method capable of safely and easily treating a medical waste which may contain harmful microbial in a medical waste container.

Provided is a method for treating a medical waste container containing a medical waste with harmful microbial, containing: a step of applying a liquid agent including chloride dioxide gas and chlorite into the medical waste container, wherein the liquid agent has a composition capable of slowly releasing the chloride dioxide gas.

Description

Disposal method of medical waste container

The present invention relates to a method for treating a medical waste container including a medical waste containing a liquid agent containing chlorine dioxide gas and chlorite and accompanied by harmful microorganisms.

In recent years, emerging/re-emerging infectious diseases such as ebola hemorrhagic fever and novel influenza have occurred all over the world and have become a major problem. The wastes (such as cloth or toilet paper attached to the patient's body fluids) that these patients with infectious diseases come into contact with, as well as the medical devices (such as syringes) used by these patients for treatment, etc., may become the source of infection, so it is necessary to Properly dispose of as medical waste.

Since medical waste may contain pathogens that have been infected by animals, it needs to be handled under a higher level of management than general waste. In general, medical waste is discarded/stored in a dedicated medical waste container, then sterilized by incineration or autoclave, or recycled/processed by professionals. However, pathogens proliferating in the medical waste container, or pathogens attached to the vicinity of the entrance of the medical waste container spread out of the medical waste container, so that it is easy to be infected when the medical waste container is handled. Risks are always present.

In many laboratories and medical institutions, in order to inactivate harmful microorganisms, wiping with alcohol-based chemicals or spray disinfection is performed. Since the bactericidal ability of volatile alcohols is low, among a wide range of alcohol-based pharmaceuticals, it is indispensable to bring the chemical solution into direct contact with the target microorganisms in order to exert its effect. That is, in a disinfection method using an alcohol-based chemical, it is difficult to sterilize microorganisms adhering to a place that the chemical cannot directly contact (for example, when the object is medical waste having a complicated three-dimensional shape).

Although chlorine dioxide gas is known to be safe for living animals at low concentrations, it has properties such as inactivation and deodorizing effects on microorganisms such as bacteria, fungi, and viruses even at such low concentrations. Although there have been proposed methods for treating medical wastes using chlorine dioxide, in order for the disinfection effect of chlorine dioxide to penetrate into every corner of the medical wastes, it is necessary to continuously expose these wastes to chlorine dioxide. Therefore, A large-scale apparatus is required (eg, Patent Document 1, Patent Document 2), or medical waste must be pulverized and immersed in a disinfecting solution containing chlorine dioxide (eg, Patent Document 3), etc., which lacks extensiveness.

(prior art literature)

(patent literature)

[Patent Document] Japanese Patent Application Laid-Open No. 5-31164

[Patent Document] Japanese Patent Application Laid-Open No. 8-24324

[Patent Document] Japanese Patent Application Laid-Open No. 6-39004

As described above, a safe and simple method for disposing of medical waste that may contain harmful microorganisms in a medical waste container has not yet been provided.

When the inventors of the present invention studied the characteristics of a solution containing chlorine dioxide, when they applied a solution containing chlorine dioxide gas and chlorite to a space, they unexpectedly found that compared with a solution containing only chlorine dioxide dissolved When the solution of chlorine gas is applied to the space, the concentration of chlorine dioxide gas in the space is maintained for a very long time (refer to Embodiment 1 of the present invention).

Therefore, the present inventors found that, by utilizing the above-mentioned properties of the solution containing chlorine dioxide gas and chlorite, a medical waste container containing medical waste can be easily and safely disposed of, and the present invention was completed. invention.

That is, the method of the present invention, in one embodiment, is a method for treating a medical waste container containing medical waste accompanied by harmful microorganisms, the method comprising applying chlorine dioxide containing chlorine dioxide to the above-mentioned medical waste container. In the step of liquid preparation of gas and chlorite, the liquid preparation has a composition for slowly releasing the chlorine dioxide gas.

Furthermore, in an embodiment of the method of the present invention, as the composition of the liquid agent, the liquid agent contains chlorine dioxide gas at a concentration of 10 to 2000 ppm and chlorous acid at a concentration of 0.05 to 10 wt %. salt, and adjust the pH of the above-mentioned liquid preparation in the range of 4.5 to 6.5.

Moreover, the method of the present invention, in one embodiment, The application of the above-mentioned liquid medicine is to spray the above-mentioned liquid medicine into the medical waste container, or to set the container containing the above-mentioned liquid medicine in the medical waste container.

Furthermore, in the method of the present invention, in one embodiment, the harmful microorganisms are infectious microorganisms.

Furthermore, in the method of the present invention, in one embodiment, the liquid preparation contains 50 to 1000 ppm of chlorine dioxide gas.

Furthermore, in the method of the present invention, in one embodiment, the liquid preparation includes 0.1% by weight to 5.0% by weight of chlorite.

Moreover, in the method of the present invention, in one embodiment, the pH of the liquid preparation is in the range of 5.5 to 6.0.

Furthermore, in an embodiment of the method of the present invention, the application of the liquid agent is such that at least a part of the harmful microorganisms are not directly contacted with the liquid agent.

Furthermore, in the method of the present invention, in one embodiment, the liquid preparation is applied so as not to directly contact the liquid preparation to all the harmful microorganisms.

Furthermore, in the method of the present invention, in one embodiment, the treatment reduces the spread of the harmful microorganisms.

In addition, inventions in any combination of one or a plurality of features of the present invention listed above are also included in the scope of the present invention.

As shown in the examples of the present application, when the liquid agent used in the method of the present invention is applied in a medical waste container, the concentration of chlorine dioxide gas in the medical waste can be maintained at a high level for a long time. Therefore, according to In the method of the present invention, since all corners of the medical waste in the medical waste container are exposed to chlorine dioxide gas, the harmful microorganisms adhering to the medical waste can be effectively treated, and the release of harmful microorganisms from the medical waste container can be reduced. diffusion. That is, in the method of the present invention, even if the applied liquid agent is not in direct contact with a part of the harmful microorganisms adhering to the medical waste (or even if the applied liquid agent has no harmful microorganisms adhering to the medical waste at all) direct contact), it can also exert a substantial disinfection effect on all harmful microorganisms in the medical waste container.

That is, the present invention provides a method of safely and simply disposing of medical waste, which may contain harmful microorganisms, in a medical waste container.

A, B: glass petri dishes

FIG. 1 is a schematic diagram of the experiment of Example 1 of the present application.

Fig. 2 is a graph showing the change over time of the chlorine dioxide concentration in the chamber when the chlorine dioxide solution of the present invention is sprayed in the chamber and when the chlorine dioxide gas solution is simply sprayed in the chamber .

FIG. 3 is a schematic diagram of the experiment of Example 2 of the present application.

Fig. 4 is a photograph of a medical waste container used in the test of Example 2 of the present application.

Fig. 5 is a flow chart showing the evaluation method of the test of Example 2 of the present application.

Fig. 6 is a graph showing the number of viable bacteria in the glass petri dish A before the test and 60 minutes after the start of the test.

Fig. 7 is a graph showing the number of viable bacteria in the glass petri dish B before the test and 60 minutes after the start of the test.

Fig. 8 is a schematic diagram of the experiment of Example 3 of the present application.

Fig. 9 shows the results of the first experiment of Example 3 of the present application.

Fig. 10 shows the results of the second experiment of Example 3 of the present application.

The present invention relates to a method for treating a medical waste container containing medical waste accompanied by harmful microorganisms.

The medical waste in this manual is not limited to the waste discharged from the relevant medical practices, but also includes the waste that can become the source of infection of infectious diseases. For example: utensils used in medical behavior (such as needles, syringes, gloves, masks, etc.), utensils used in biological experiments (such as: petri dishes with microorganisms, culture medium, microchips, etc.), blood of patients with infectious diseases And body fluid tissue or cloth, etc., are also included in the medical waste in this manual.

The medical waste container in this specification is a container for storing medical waste, and its shape and structure are not particularly limited. From the viewpoint of preventing the spread of infectious microorganisms, the medical waste container to which the present invention is applied is preferably one with high airtightness. However, even if it is not completely airtight, as long as it has a certain degree of airtight structure, The present invention can also be appropriately applied. Furthermore, the method of the present invention can also be used in a medical waste container having a plurality of compartments as long as the compartments are in fluid communication (when the compartments are not in fluid communication, the application of the method of the present invention between the compartments , target effect).

The method of the present invention includes the step of applying a liquid preparation containing chlorine dioxide gas and chlorite in a medical waste container. The liquid preparation used in the method of the present invention contains chlorine dioxide gas at a concentration of 10 to 2000 ppm and chlorite at a concentration of 0.05 to 10 wt %, and the pH of the above liquid preparation is adjusted in the range of 4.5 to 6.5 The liquid preparation is better. Preferably, the concentration of chlorine dioxide gas contained in the liquid preparation may be 50 to 1000 ppm, and more preferably may be 100 to 600 ppm. Moreover, the concentration of the chlorite contained in the liquid preparation may preferably be 0.1% by weight to 5.0% by weight, and more preferably 0.5% by weight to 2.5% by weight. When the pH of the solution is lower than 4.5, the chlorite in the solution reacts excessively to release chlorine dioxide gas, so it is difficult to control the amount of chlorine dioxide gas released, and the storage stability of the solution decreases. In addition, when the pH of the liquid agent is higher than 6.5, the reactivity of the chlorite in the liquid agent is lowered, and an appropriate amount of chlorine dioxide gas cannot be released. The pH of the liquid preparation is preferably in the range of 5.5 to 6.0. In addition, the chlorine dioxide gas concentration, the chlorite concentration, and the pH in the liquid agent can be arbitrarily combined within the above-mentioned ranges.

The liquid preparation used in the method of the present invention can be produced, for example, as follows. First, (a) chlorite is dissolved in water to prepare an aqueous chlorite solution of 2000 to 180000 ppm, (b) chlorine dioxide gas is dissolved to prepare an aqueous chlorine dioxide solution of 100 to 2900 ppm, and (a) ) and (b) were mixed, and a pH adjuster was mixed with the solution to prepare a chlorine dioxide solution. In addition, in the above-mentioned production method, the concentration of the chlorite aqueous solution and the concentration of the chlorine dioxide aqueous solution can be appropriately adjusted according to the composition of the intended liquid preparation by those with ordinary knowledge in the technical field.

By adjusting the liquid preparation as described above, the concentration of chlorine dioxide dissolved in the liquid preparation can be freely adjusted from a high concentration to a low concentration. Furthermore, since the liquid agent used in the present invention contains chlorine dioxide gas and chlorite, when the chlorine dioxide gas is released from the liquid agent into the air, the concentration of chlorine dioxide gas in the liquid agent will decrease, However, due to chemical equilibrium, chlorine dioxide is supplied from the chlorite to the liquid agent, and as a result, the chlorine dioxide gas concentration in the liquid agent is kept substantially constant. With this effect, the liquid preparation used in the present invention can slowly release chlorine dioxide gas into the air over a long period of time. Furthermore, when the pH of the liquid agent is adjusted within the range of 4.5 to 6.5, the balance between the amount of chlorine dioxide gas released from the liquid agent and the supply of chlorine dioxide from the chlorite is properly maintained, so that it is possible to A roughly constant concentration of chlorine dioxide gas is released over a longer period of time.

Examples of the chlorites contained in the liquid preparation used in the method of the present invention include alkali metal chlorites and alkaline earth metal chlorites. Examples of the alkali metal chlorite salts include sodium chlorite, potassium chlorite, and lithium chlorite, and examples of the alkaline earth metal chlorite salts include calcium chlorite, magnesium chlorite, and barium chlorite. . Among them, from the point of being easy to obtain, sodium chlorite and potassium chlorite are preferable, and sodium chlorite is the best. These chlorites may be used alone or in combination of two or more.

As the pH adjuster for preparing the liquid preparation used in the method of the present invention, anyone with general knowledge in the technical field can use any one, but for example, phosphoric acid, boric acid, metaphosphoric acid, pyrophosphoric acid, sulfamic acid can be used , acetic acid, citric acid, and their salts, etc., from the viewpoint of obtaining excellent storage stability, inorganic acids or their salts are preferred. Among them, from the preservation of stability It is excellent in properties, and the fluctuation of the liquid (pH) during storage can be minimized, so that the excellent bactericidal, antiviral, antifungal, and deodorant effects can be exhibited. Phosphoric acid or Its salts (eg: sodium dihydrogen phosphate, a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, etc.) are preferred, and it is more preferred to use sodium dihydrogen phosphate. In addition, a pH adjuster may be used individually by 1 type, and may use 2 or more types together.

In the method of the present invention, the method for applying the liquid preparation in the medical waste container is arbitrary, for example, the liquid preparation can be applied by spraying the above liquid preparation in the medical waste container, and it can also be applied by spraying the liquid preparation containing the above liquid preparation in the medical waste container. The container of the agent is arranged in a medical waste container for application. From the viewpoint of immediate effect through application, and the effect can last for a longer period of time, it is better to use a liquid formulation by spraying.

Moreover, as also shown in the examples of the present application, when the liquid agent used in the method of the present invention is applied in a medical waste container, the concentration of chlorine dioxide gas in the medical waste can be maintained at a high level for a long time. Therefore, according to the method of the present invention, all corners of the medical waste in the medical waste container are exposed to chlorine dioxide gas, so the harmful microorganisms attached to the medical waste can be effectively treated, and the self-medication of the harmful microorganisms can be reduced. Diffusion of waste containers. That is, in the method of the present invention, even if the applied liquid agent is not in direct contact with a part of the harmful microorganisms adhering to the medical waste (or even if the applied liquid agent is completely in contact with the harmful microorganisms adhering to the medical wastes) Without direct contact), it can also exert a substantial disinfection effect on all harmful microorganisms in the medical waste container.

The harmful microorganisms in the method of the present invention include a wide range of Contains microorganisms that can cause adverse effects on humans or animals, and in particular, contains microorganisms that are infectious to humans or animals. For example, harmful microorganisms in the present invention include: viruses, bacteria, fungi, archaea and parasites (for example: parasites, parasitic arthropods), infectious proteins (for example: Abnormal prion protein) )). Viruses to which the method of the present invention can be applied include enveloped viruses or non-enveloped viruses, such as chickenpox/herpes zoster virus, influenza virus (human, bird and pig, etc.), herpes simplex type Virus, adenovirus, enterovirus, rhinovirus, human papillomavirus (human papillomavirus), poxvirus, coxsackie virus, herpes simplex virus (HSV: herpes simplex virus), cytomegalovirus, Epstein-Barr virus, adenovirus Virus, papilloma virus, JC virus, parvovirus, hepatitis B virus, hepatitis C virus, lyssa virus, feline calicivirus, norovirus, sapovirus, coronavirus, SARS virus, rubella virus, Mumps virus, Measles virus, RS virus, Polio virus, Coxsackie virus, Echo virus, Marburg virus, Ebola virus, Yellow fever virus, Benyanviridae, Rabies virus, Rio Viridae virus, rotavirus, human immunodeficiency virus (HIV), human T lymphocytic leukemia virus, simian immunodeficiency virus and STLV, etc. Furthermore, the bacteria to which the method of the present invention can be applied include Gram-positive bacteria or Gram-negative bacteria, for example, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus, Neisseria gonorrhoeae, Syphilis, Meningococcus, Mycobacterium tuberculosis, Acid-fast bacteria, Klebsiella (pneumonia), Salmonella, Clostridium botulinum, Proteus, Haemophilus pertussis, Serratia, Vibrio enteritidis, Citrobacter, Acinetobacter Bacillus, Aspergillus, Enterobacter, Mycoplasma, Chlamydia and Clostridium Wait. Furthermore, fungi to which the method of the present invention can be applied include, for example, Koji fungi, Trichophyton, Malassezia fungi, Candida, and the like.

The terms used in this specification are used to describe specific embodiments, and are not intended to limit the present invention.

In addition, the term "comprises" used in this specification, unless there is a clearly different interpretation in the text, intends that the stated items (components, steps, requirements, numbers, etc.) are all present, and does not exclude other matters ( components, steps, elements or numbers, etc.).

Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as widely understood by one of ordinary skill in the art to which this invention belongs. Terms used herein, unless otherwise defined, shall be interpreted as having meanings consistent with the meanings in this specification and related technical fields, and shall not be interpreted as idealized or excessively formalized.

The embodiments of the present invention are described with reference to the schematic diagrams. In the schematic diagrams, for the sake of clarity, the descriptions may be exaggerated.

In this specification, for example, when "1 to 10%" is expressed, those with ordinary knowledge in the technical field should understand the expression as individually and specifically indicating 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10%.

In the present specification, any numerical value used in the presentation of component contents and numerical ranges shall be interpreted as including the meaning of the term "about" unless otherwise specified. For example: "10 times", unless expressly stated otherwise, should be interpreted as "about 10 times".

Documents cited in this specification, all disclosures of such It should be regarded as a reference in this specification, and those with general knowledge in the technical field should understand the relevant disclosures in the prior art documents according to the context of this specification without departing from the spirit and scope of the present invention. The contents are incorporated as part of this manual.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the present invention can be embodied in various forms, and should not be construed as being limited to the embodiments described therein.

[Example]

Example 1: Continuous effect of chlorine dioxide concentration in the space of the chlorine dioxide solution of the present invention

<Preparation of chlorine dioxide solution>

The chlorine dioxide solution of the present invention is prepared as follows. First, 17 L of water was added to 500 ml of a 25% by weight solution of sodium chlorite to prepare an aqueous sodium chlorite solution. Then, 1000 ml of chlorine dioxide aqueous solution in which 2000 ppm of chlorine dioxide gas was dissolved was prepared, and then mixed with the above-mentioned sodium chlorite aqueous solution and stirred. Next, sodium dihydrogen phosphate and water are added in such an amount that the pH of the solution becomes 5.5 to 6.0, followed by stirring. This operation was performed to obtain 20L of chlorine dioxide solution containing chlorine dioxide gas, sodium chlorite and sodium dihydrogen phosphate (that is, to obtain 0.625 wt % of sodium chlorite, 100 ppm of chlorine dioxide gas and a pH of 5.5 liquid to 6.0). In this embodiment, the chlorine dioxide solution is called "the chlorine dioxide solution of the present invention".

In this example, the chlorine dioxide gas solution system used as a comparative example was prepared by dissolving chlorine dioxide gas generated by adding hydrochloric acid to sodium chlorite in water (that is, simply Chlorine oxide solution of gas dissolved in water). In this embodiment, the solution is called "chlorine dioxide gas solution".

<Experimental method>

FIG. 1 shows the outline of the experiment of this embodiment. A chamber of 1 m ³ is prepared, and the chlorine dioxide solution or chlorine dioxide gas solution of the present invention is sprayed three times through a sprayer. While stirring the air in the chamber by two fans, the concentration of chlorine dioxide gas in the chamber was measured over time with a gas detector.

<result>

The experimental results are shown in Figure 2. As shown in Fig. 2, when the chlorine dioxide gas solution was sprayed, the chlorine dioxide gas concentration decreased rapidly, and after 1.5 hours, it was less than the detection limit. On the other hand, when the chlorine dioxide solution of the present invention was sprayed, the gas concentration was maintained for about 2 hours, then gradually decreased, and about 40% of the initial concentration was maintained after 8 hours.

That is, surprisingly, when the chlorine dioxide liquid agent of the present invention is sprayed in a space, it exhibits the characteristic of maintaining the chlorine dioxide gas concentration for a very long time in the space.

Example 2: Sterilization of microorganisms in a medical waste container using the chlorine dioxide solution of the present invention

<Experimental method>

FIG. 3 shows the outline of the experiment of this embodiment. In addition, the chlorine dioxide solution used in this example was prepared by the same method as described in Example 1.

First, a container (40L) for medical waste used in a hospital or the like is prepared (Fig. 4), and a compartment (to 2/3 of the height of the container) is provided in the center of the container. A Petri dish with E. coli attached facing upwards was placed in one compartment (Setup A), and an identical Petri dish facing downwards was placed in the other area (Setup B). Spray 83% ethanol or the chlorine dioxide solution of the present invention on the petri dish of setting A, and after 1 hour, recover Escherichia coli from the petri dish of setting A and setting B, and measure the number of viable bacteria according to conventional methods. An outline of the evaluation method is shown in Figure 5.

<result>

The experimental results are shown in FIGS. 6 and 7 . As shown in Fig. 6 and Fig. 7, in setting A, 83% ethanol and the chlorine dioxide solution of the present invention both inactivated E. coli, while in setting B (the opposite area of the spray area), only this The chlorine dioxide solution of the invention can inactivate Escherichia coli. The chlorine dioxide gas concentration in the container when the chlorine dioxide liquid agent was sprayed was 3.3 ppm. As a result, it is suggested that chlorine dioxide gas is generated from the sprayed chlorine dioxide liquid agent, and by the gas, Escherichia coli, which is placed at a distance and facing downward, is inactivated.

That is, according to the chlorine dioxide solution of the present invention, not only the microorganisms at the place where the solution is directly applied can be sterilized, but the concentration of chlorine dioxide in the container of the solution solution is maintained for a long period of time, so it can be Disinfect the places where the liquid agent is not directly applied (for example, the corners of the container where the liquid agent does not directly reach and the corners of the waste with complex structure). Moreover, according to the chlorine dioxide solution of the present invention, even if it is not directly applied to the medical waste to be sterilized, as long as the solution is applied to the specific space of the container in which the medical waste is accommodated, the treatment of the medical waste can be indirectly affected. Play in every corner Therefore, it is possible to provide a disinfection method that minimizes the risk of medical personnel and the like coming into contact with medical waste.

For example, when infectious diseases with a high fatality rate such as novel influenza, Ebola hemorrhagic fever, and MERS are prevalent, it is predicted that a large amount of wastes with suspected sources of infection will be produced in a short period of time. When the method of the present invention is applied to a container or the like containing such wastes, the source of infection can be more easily and safely blocked (or the risk of the spread of harmful microorganisms from the waste container can be reduced).

Example 3: Inactivation of viruses in medical waste containers using the chlorine dioxide solution of the present invention

<Materials and methods>

Test virus: Feline calicivirus (feline calicivirus, FCV, F9, ATCC VR-782) (used as a substitute for norovirus)

Host cell: Crandell Rees feline kidney cells (CRFK, ATCC, CCL-94)

Chlorine dioxide gas generation source: a chlorine dioxide solution prepared in the same manner as described in Example 1

labware

‧1m ³ chamber (ordered item)

‧96-well microtiter plate (353072, FALCON)

‧96-well deep-well plate (BM6030, BM Bio)

‧Reagent storage container/Tip-Tub (022265806, eppendorf)

‧AC fan (MU 825S-13N, ORIX)

‧Glass petri dish (305-02,Top)

‧Cell scraper (179693, Thermo)

‧Gas detector (No. 23L chlorine dioxide, GASTEC)

experimental machine

‧CO ₂ incubator (MCO-175 AICUVH, PANASONIC)

‧Temperature and Hygrometer (TR-72 wf, T&D)

Experimental Materials

‧DMEM high glucose (Dulbecco's Modified Eagle's Medium-high glucose, D-MEM, D 5796, SIGMA)

‧Dulbecco’s Phosphate Buffered Saline (D8537, SIGMA)

‧0.25% Trypsin Solution (0.25% Trypsin Solution, 35555-54, NACALAI TESQUE)

‧Fetal Bovine Serum (FBS, 30-2020, ATCC)

‧2% solution of EDTA disodium salt in PBS saline (2820549, MP)

Adjustment of Virus Suspension:

Feline calicivirus (10 ^8.5 TCID ₅₀ /50 μL) frozen at -80°C was diluted with 1% FBS solution to 10 ^7-7.5 TCID ₅₀ /50 μL as virus suspension.

Virus recovery solution (neutralization solution):

120 μL of 2% FBS D-MEM (anti+) containing 1 mM sodium thiosulfate solution (*) was used as a virus recovery solution.

(*) 1 mM sodium thiosulfate solution is the concentration at which 0.1 ppmv of chlorine dioxide gas can be neutralized without problems.

Experimental method (refer to Figure 8):

A petri dish into which 1 μl of feline calicivirus suspension (10 ^7-7.5 TCID ₅₀ /50 μL) supplemented with 1% FBS (fetal bovine serum) was added was set at the position of B in a 1 m ³ chamber. After setting, the chlorine dioxide solution of the present invention is sprayed at about 70cm away from the set petri dish. After the dropwise FCV is exposed and neutralized by the chlorine dioxide gas produced by spraying the chlorine dioxide solution of the present invention at each time, the virus is recovered, and the virus infection titer (Virus Infectivity Titer) of each time is obtained. and assessed. Similarly, water was used to replace the chlorine dioxide liquid spray of the present invention as a control group.

Chlorine dioxide gas concentration:

The concentration of chlorine dioxide gas in the 1m ³ chamber was measured through a detection tube.

<result>

The experiment was carried out twice.

The results of the first experiment are shown in FIG. 9 . In the first experiment, when the chlorine dioxide solution of the present invention was sprayed, the average concentration of chlorine dioxide gas in the chamber was 0.1 ppmv (minimum value: 0.075 ppmv, maximum value: 0.1 ppmv). In the control group in which the chlorine dioxide solution of the present invention was sprayed with water, the virus titer after 10 minutes of spraying was 10 ^6.3 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it became 10 ^2.8 TCID _50/50 μL (3.5 log ₁₀ reduction). Furthermore, 15 minutes after spraying, the virus titer in the control group was 10 ^4.5 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it became 10 ^2.3 TCID ₅₀ /50 μL (reduction by 2.2 log ₁₀ ). After 30 minutes of spraying, the virus titer in the control group was 10 ^5.5 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it became 10 ^1.0 TCID ₅₀ /50 μL (reduction by 4.5 log ₁₀ ).

The results of the second experiment are shown in FIG. 10 . In the second experiment, when the chlorine dioxide solution of the present invention was sprayed, the chlorine dioxide gas concentration in the chamber was 0.1 ppmv on average (minimum value: 0.1 ppmv, maximum value: 0.2 ppmv). In the control group in which the chlorine dioxide solution of the present invention was sprayed with water, the virus titer after 10 minutes of spraying was 10 ^5.5 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it became 10 ^0.8 TCID _50/50 μL (4.7 log ₁₀ reduction). Furthermore, 15 minutes after spraying, the virus titer in the control group was 10 ^4.3 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it was 10 ^1.7 TCID ₅₀ /50 μL (reduction by 2.6 log ₁₀ ). After 30 minutes of spraying, the virus titer in the control group was 10 ^3.9 TCID ₅₀ /50 μL, but when the chlorine dioxide solution of the present invention was sprayed, it became 10 ^0.5 TCID ₅₀ /50 μL (reduction by 3.4 log ₁₀ )

From the above, it was proved that the present invention is applicable not only to bacteria but also to viruses.

This representative figure has no component symbols and their meanings.

Claims (6)

A processing method of a medical waste container, the medical waste container contains medical waste accompanied by harmful microorganisms, and the processing method comprises the following steps: The step of applying a liquid preparation containing chlorine dioxide gas and chlorite in the above-mentioned medical waste container, wherein, The aforementioned liquid preparation contains chlorine dioxide gas with a concentration of 10 to 2000 ppm and 0.05 to 10 wt% of chlorite, and the pH of the aforementioned liquid preparation is adjusted within the range of 4.5 to 6.5, The application of the liquid agent is to spray the liquid agent into the medical waste container, or set the container containing the liquid agent in the medical waste container, The aforementioned medical waste is discarded after the aforementioned treatment and is no longer used. The method for treating medical waste containers as described in item 1 of the scope of the application, wherein, The aforementioned harmful microorganisms are infectious microorganisms. According to the treatment method of medical waste container described in item 1 or item 2 of the scope of application, wherein, The aforementioned liquid preparation contains 50 to 1000 ppm of chlorine dioxide gas. The method for treating medical waste containers as described in any one of items 1 to 3 of the scope of the application, wherein, The aforementioned liquid formulation contains 0.1% by weight to 5.0% by weight of chlorite. The method for treating medical waste containers as described in any one of items 1 to 4 of the scope of the application, wherein, The pH of the aforementioned liquid preparation is in the range of 5.5 to 6.0. The method for treating medical waste containers as described in any one of items 1 to 5 of the scope of the application, wherein, The aforementioned treatment reduces the spread of the aforementioned harmful microorganisms.

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