JPWO2017090570A1 - Treatment method for medical waste containers - Google Patents

Abstract

【Task】
Provided is a method for safely and simply treating medical waste that may contain harmful microorganisms in a medical waste container.
[Solution]
A method for treating a medical waste container containing medical waste accompanied by harmful microorganisms, comprising a step of applying a liquid agent containing chlorine dioxide gas and chlorite in the medical waste container, Provided is a method characterized by comprising a composition for sustained release of chlorine dioxide gas.
[Selection] Figure 7

Description

  The present invention relates to a method for treating a medical waste container containing medical waste accompanied by harmful microorganisms using a liquid agent containing chlorine dioxide gas and chlorite.

  In recent years, emerging and re-emerging infectious diseases such as Ebola hemorrhagic fever and new influenza have occurred in various parts of the world and have become major problems. Wastes contacted by patients with these infections (for example, cloth or tissue paper with the patient's bodily fluid), medical devices used to treat these patients (for example, syringes), etc. Therefore, it needs to be properly treated as medical waste.

  Since medical waste can include pathogens that infect humans and animals, it needs to be treated under higher control than general waste. Generally, medical waste is disposed / stored in a dedicated medical waste container and then sterilized by incineration or autoclave, or collected / processed by a specialist. However, pathogens that grow in medical waste containers or that adhere to the vicinity of the entrance of medical waste containers can spread out of the medical waste containers, creating a risk of infection when handling medical waste containers. Sex always exists.

  In many laboratories and medical institutions, in order to inactivate harmful microorganisms, disinfection by wiping or spraying with an alcohol-based drug is performed. Since the sterilizing ability of the volatilized alcohol is low, it is indispensable for the drug solution to be in direct contact with the target microorganism in order to exert its effect in the alcohol-based chemicals that are widely used. That is, in the disinfection method using an alcohol-based drug, it is difficult to disinfect microorganisms adhering to a place where the drug is not in direct contact (for example, in the case of medical waste having a complicated tertiary shape, etc).

  Chlorine dioxide gas is a safe gas for animal bodies at low concentrations, but even at such low concentrations, it has a deactivating effect and a deodorizing effect on bacteria such as bacteria, fungi, and viruses. It is known that The treatment method of medical waste using chlorine dioxide has been proposed so far, but in order to penetrate the disinfection effect of chlorine dioxide to every corner of medical waste, it is necessary to continuously expose to chlorine dioxide. For this purpose, a large-scale device is required (for example, Patent Document 1 and Patent Document 2), or medical waste must be pulverized and immersed in a disinfectant containing chlorine dioxide (for example, Patent Document 3). ) Etc., it was poor in versatility.

JP 5-31164 JP-A-8-24324 JP-A-6-39004

  As described above, a method for safely and simply treating medical waste that may contain harmful microorganisms in a medical waste container has not been provided so far.

  The present inventors have studied the properties of a solution containing chlorine dioxide, and surprisingly, when a solution containing chlorine dioxide gas and chlorite was applied to the space, only chlorine dioxide gas was dissolved. It has been found that the chlorine dioxide gas concentration in the space is maintained for a very long time compared to the case where the solution is applied to the space (see Example 1 of the present application).

  And the present inventors can process a medical waste container containing medical waste simply and safely by utilizing the above properties of a solution containing chlorine dioxide gas and chlorite. As a result, the present invention has been completed.

  That is, in one embodiment, the method of the present invention is a method for treating a medical waste container containing medical waste accompanied by harmful microorganisms, wherein the medical waste container contains a liquid solution containing chlorine dioxide gas and chlorite. The liquid agent is provided with a composition that releases the chlorine dioxide gas gradually, and relates to a method.

  In one embodiment of the method of the present invention, as the composition of the liquid agent, the liquid agent includes 10 to 2000 ppm of chlorine dioxide gas and 0.05 to 10% by weight of chlorite. And the pH of the solution is adjusted within the range of 4.5 to 6.5.

  In one embodiment, the method of the present invention is characterized in that the application of the liquid is performed by spraying the liquid into a medical waste container or installing a container containing the liquid into the medical waste container. And

  In one embodiment, the method of the present invention is characterized in that the harmful microorganism is an infectious microorganism.

  Moreover, the method of this invention is characterized by the said liquid agent containing 50-1000 ppm chlorine dioxide gas in one embodiment.

  Moreover, the method of this invention is characterized by the said liquid agent containing 0.1 weight%-5.0 weight% chlorite in one embodiment.

  Moreover, the method of this invention is characterized by the pH of the said liquid agent being in the range of 5.5-6.0 in one embodiment.

  In one embodiment, the method of the present invention is characterized in that the liquid agent is not in direct contact with at least a part of the harmful microorganisms.

  In one embodiment, the method of the present invention is characterized in that the solution is not in direct contact with all harmful microorganisms.

  In one embodiment, the method of the present invention is characterized in that the treatment reduces diffusion of the harmful microorganisms.

  Note that an invention in which one or more features of the present invention listed above are arbitrarily combined is also included in the scope of the present invention.

  As also shown in the examples of the present application, when the liquid used in the method of the present invention is applied to a medical waste container, the chlorine dioxide gas concentration in the medical waste is kept high for a long time. Therefore, according to the method of the present invention, since every corner of the medical waste in the medical waste container is exposed to chlorine dioxide gas, harmful microorganisms attached to the medical waste can be treated efficiently, The diffusion of harmful microorganisms from the waste container can be reduced. That is, in the method of the present invention, the applied liquid agent does not directly contact a part of the harmful microorganisms attached to the medical waste (or the applied liquid agent is directly applied to the microorganisms attached to the medical waste. Disinfectant effect on virtually all harmful microorganisms in the medical waste container.

  That is, the present invention provides a method for safely and simply treating medical waste that may contain harmful microorganisms in a medical waste container.

FIG. 1 is a schematic diagram of a test of Example 1 of the present application. FIG. 2 is a graph showing the time course of the chlorine dioxide concentration in the chamber when the chlorine dioxide solution of the present invention is sprayed into the chamber and when a simple chlorine dioxide gas dissolved solution is sprayed into the chamber. It is. FIG. 3 is a schematic diagram of the test of Example 2 of the present application. FIG. 4 is a photograph of a medical waste container used in the test of Example 2 of the present application. FIG. 5 is a diagram showing the flow of the test evaluation method of Example 2 of the present application. FIG. 6 is a graph showing the number of bacteria survived on glass petri dish A before the test and 60 minutes after the start of the test. FIG. 7 is a graph showing the bacterial viability of glass petri dish B before the test and 60 minutes after the start of the test. FIG. 8 is a schematic diagram of an experiment of Example 3 of the present application. FIG. 9 shows the results of the first experiment in Example 3 of the present application. FIG. 10 shows the results of the second experiment in Example 3 of the present application.

  The present invention relates to a method for treating a medical waste container containing medical waste accompanied by harmful microorganisms.

  The medical waste in this specification is not limited to the waste discharged in relation to medical practice, but includes any waste that can be a source of infection. For example, instruments used in medical practice (eg, needles, syringes, gloves, masks, etc.), instruments used in biological experiments (eg, petri dishes, culture media, microchips, etc.) Papers and cloths to which blood and body fluids of infectious patients are attached are also included in the medical waste in this specification.

  The medical waste container in this specification is a container for storing medical waste, and its shape and structure are not particularly limited. From the viewpoint of preventing the spread of infectious microorganisms, it is preferable that the medical waste container to which the present invention is applied be highly airtight. The present invention can be preferably applied. The method of the present invention can also be applied to a medical waste container having a plurality of compartments as long as each compartment is in fluid communication (when each compartment is not in fluid communication). Can be obtained by applying the method of the present invention to each compartment).

  The method of the present invention includes the step of applying a solution comprising chlorine dioxide gas and chlorite in a medical waste container. The liquid used in the method of the present invention contains 10 to 2000 ppm of chlorine dioxide gas, 0.05 wt% to 10 wt% chlorite concentration, and the pH of the liquid is 4.5. It is preferable that it is the liquid agent prepared in the range of -6.5. Preferably, the concentration of chlorine dioxide gas contained in the liquid agent may be 50 to 1000 ppm, more preferably 100 to 600 ppm. Preferably, the concentration of chlorite contained in the solution may be 0.1 wt% to 5.0 wt%, more preferably 0.5 wt% to 2.5 wt%. Good. If the pH of the solution is lower than 4.5, the chlorite in the solution reacts excessively and releases chlorine dioxide gas, making it difficult to control the amount of chlorine dioxide gas released, and the storage stability of the solution Sex is reduced. Moreover, when the pH of a liquid agent becomes higher than 6.5, the reactivity of the chlorite in a liquid agent will fall, and an appropriate amount of chlorine dioxide gas will not be discharge | released. The pH of the liquid agent is more preferably in the range of 5.5 to 6.0. In addition, the chlorine dioxide gas density | concentration in a liquid agent, a chlorite density | concentration, and pH can be arbitrarily combined in said range.

  The liquid agent used in the method of the present invention can be produced, for example, as follows. First, (a) chlorite is dissolved in water to prepare 2000-18000 ppm chlorite aqueous solution, (b) chlorine dioxide gas is dissolved to prepare 100-2900 ppm chlorine dioxide aqueous solution, and After mixing (a) and (b), a pH adjuster is mixed with this solution to obtain a chlorine dioxide solution. In addition, the concentration of the chlorite aqueous solution and the concentration of the chlorine dioxide aqueous solution in the above production method can be appropriately adjusted by those skilled in the art according to the composition of the target liquid agent.

  By adjusting the liquid agent by the method as described above, the dissolved chlorine dioxide concentration in the liquid agent can be freely adjusted from a high concentration to a low concentration. Moreover, since the liquid agent used in the method of the present invention contains chlorine dioxide gas and chlorite, when chlorine dioxide gas is released into the air from the liquid agent, the chlorine dioxide gas concentration in the liquid agent decreases, Due to chemical equilibrium, chlorine dioxide is supplied from the chlorite into the liquid, and as a result, the chlorine dioxide gas concentration in the liquid is kept substantially constant. Due to this effect, the liquid agent used in the present invention can release chlorine dioxide gas into the air gradually over a long period of time. In addition, when the pH of the solution is adjusted within the range of 4.5 to 6.5, the balance between the amount of chlorine dioxide gas released from the solution and the supply of chlorine dioxide from chlorite is suitably maintained. Therefore, chlorine dioxide gas having a substantially constant concentration can be released for a longer time.

  Examples of the chlorite contained in the liquid used in the method of the present invention include an alkali metal chlorite and an alkaline earth metal chlorite. Examples of the alkali metal chlorite include sodium chlorite, potassium chlorite, and lithium chlorite. Examples of the alkaline earth metal chlorite include calcium chlorite, magnesium chlorite, Barium chlorate is mentioned. Of these, sodium chlorite and potassium chlorite are preferable and sodium chlorite is most preferable from the viewpoint of easy availability. These chlorites may be used individually by 1 type, and may use 2 or more types together.

  A person skilled in the art can use any pH adjusting agent for preparing the liquid used in the method of the present invention. For example, phosphoric acid, boric acid, metaphosphoric acid, pyrophosphoric acid, sulfamic acid, acetic acid, Citric acid or a salt thereof can be used, and an inorganic acid or a salt thereof is preferable in that excellent storage stability can be obtained. In particular, it has excellent storage stability and can minimize fluctuations in liquidity (pH) during storage, thereby exhibiting excellent bactericidal, antiviral, antifungal, and deodorizing effects. It is preferable to use phosphoric acid or a salt thereof (for example, sodium dihydrogen phosphate, a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, etc.), and sodium dihydrogen phosphate. More preferably, is used. In addition, a pH adjuster may be used individually by 1 type, and may use 2 or more types together.

  In the method of the present invention, the method of applying the liquid agent into the medical waste container is arbitrary. For example, the liquid agent may be applied by spraying the medical agent into the medical waste container and includes the liquid agent. You may apply by installing a container in a medical waste container. From the viewpoint that the effect is exhibited immediately after application and the effect lasts for a relatively long time, it is more preferable to apply the solution by spraying.

  As also shown in the examples of the present application, when the liquid used in the method of the present invention is applied in a medical waste container, the chlorine dioxide gas concentration in the medical waste is kept high for a long time. It is. Therefore, according to the method of the present invention, since every corner of the medical waste in the medical waste container is exposed to chlorine dioxide gas, harmful microorganisms attached to the medical waste can be treated efficiently, The diffusion of harmful microorganisms from the waste container can be reduced. That is, in the method of the present invention, the applied liquid agent does not directly contact a part of the harmful microorganisms attached to the medical waste (or the applied liquid agent is directly applied to the microorganisms attached to the medical waste. Disinfectant effect on virtually all harmful microorganisms in the medical waste container.

  The harmful microorganisms in the method of the present invention widely include microorganisms that can adversely affect humans and animals, and particularly include microorganisms that are infectious to humans and animals. For example, harmful microorganisms in the present invention include viruses, bacteria, fungi, archaea, parasites (eg, parasites, parasitic arthropods), and infectious proteins (eg, abnormal prion proteins). It is. Viruses to which the method of the present invention can be applied include enveloped or non-enveloped viruses such as varicella / zoster virus, influenza viruses (human, bird, swine, etc.), herpes simplex virus, adenovirus, enterovirus. , Rhinovirus, human papilloma virus (human papillomavirus), pox virus, coxsackie virus, herpes simplex virus, cytomegalovirus, EB virus, adenovirus, papilloma virus, JC virus, parvovirus, hepatitis B virus, hepatitis C virus , Lassa virus, feline calicivirus, norovirus, sapovirus, coronavirus, SARS virus, rubella virus, mumps virus, measles virus, RS virus, poliovirus Coxsackie virus, Echo virus, Marburg virus, Ebola virus, Yellow fever virus, Bunyaviridae virus, Rabies virus, Reoviridae virus, Rotavirus, Human immunodeficiency virus, Human T lymphophilic virus, Monkey immunodeficiency virus , STLV and the like. Examples of bacteria to which the method of the present invention can be applied include Gram-positive bacteria or Gram-negative bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus, Neisseria gonorrhoeae, syphilis, meningococcus, tuberculosis, antibacterial Acid bacteria, Klebsiella (Klebsiella pneumoniae), Salmonella, Clostridium botulinum, Proteus, Bordetella pertussis, Vibrio parahaemolyticus, Citrobacter, Acinetobacter, Campylobacter, Enterobacter, Mycoplasma, Chlamydia, Clostridium and the like. Furthermore, examples of fungi to which the method of the present invention can be applied include Aspergillus, ringworm, Malassezia, and Candida.

  The terminology used herein is for the purpose of describing particular embodiments and is not intended to be limiting of the invention.

  In addition, the term “comprising” as used in this specification intends that there is a matter (a member, a step, an element, a number, or the like) described unless the context clearly requires different understanding. It does not exclude the presence of other items (members, steps, elements, numbers, etc.).

  Unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms used herein should be construed as having a meaning consistent with the meaning in this specification and the related technical field, unless otherwise defined, idealized, or overly formal. It should not be interpreted in a general sense.

  Embodiments of the present invention may be described with reference to schematic diagrams, but in the case of schematic diagrams, they may be exaggerated for clarity of explanation.

  In this specification, for example, when expressed as “1 to 10%”, those skilled in the art will recognize that the expression is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10%. Understand what it refers to individually.

  In this specification, any numerical value used to indicate component content or numerical range is to be interpreted as including the meaning of the term “about” unless otherwise indicated. For example, “10 times” is understood to mean “about 10 times” unless otherwise specified.

  References cited in this specification are to be regarded as the entire disclosure of which is hereby incorporated by reference, and those skilled in the art will recognize the spirit of the invention in accordance with the context of this specification. And without departing from the scope, the relevant disclosures in those prior art documents are incorporated and understood as part of the present specification.

  In the following, the present invention will be described in more detail with reference to examples. However, the invention can be embodied in various ways and should not be construed as limited to the embodiments set forth herein.

Example 1 : Effect of sustaining chlorine dioxide concentration in the space of the chlorine dioxide solution of the present invention <Preparation of chlorine dioxide solution>
The chlorine dioxide solution according to the present invention was prepared as follows. First, 17 L of water was added to 500 ml of a 25 wt% sodium chlorite solution to prepare a sodium chlorite aqueous solution. Thereafter, 1000 ml of a chlorine dioxide aqueous solution in which 2000 ppm of chlorine dioxide gas was dissolved was prepared, mixed with the sodium chlorite aqueous solution and stirred. Next, sodium dihydrogen phosphate and water in such an amount that the pH of the solution was 5.5 to 6.0 were added and stirred. Thus, 20 L of chlorine dioxide solution containing chlorine dioxide gas, sodium chlorite, and sodium dihydrogen phosphate was obtained (that is, containing 0.625% by weight of sodium chlorite and 100 ppm of chlorine dioxide gas). And a liquid having a pH of 5.5 to 6.0 was obtained). In this embodiment, this chlorine dioxide solution is referred to as “the chlorine dioxide solution of the present invention”.

  The chlorine dioxide gas dissolved solution used as a comparative example in this example was prepared by dissolving chlorine dioxide gas generated by adding hydrochloric acid to sodium chlorite in water (that is, simply chlorine dioxide). It is a solution in which gas is dissolved in water). In this embodiment, this solution is referred to as “chlorine dioxide gas dissolved solution”.

<Experiment method>
FIG. 1 shows an outline of the experiment of this example. A 1 m ³ chamber was prepared, and the chlorine dioxide solution of the present invention or the chlorine dioxide gas dissolved solution was sprayed three times by spraying. While stirring the air in the chamber with two fans, the chlorine dioxide gas concentration in the chamber was measured over time with a gas detector tube.

<Result>
The experimental results are shown in FIG. As shown in FIG. 2, when the chlorine dioxide gas dissolved solution was sprayed, the chlorine dioxide gas concentration rapidly decreased and became below the detection limit after 1.5 hours. On the other hand, when the chlorine dioxide solution of the present invention was sprayed, the gas concentration was maintained until about 2 hours and then gradually decreased, and about 40% of the initial concentration was maintained even after 8 hours.

  That is, it was surprisingly shown that the chlorine dioxide solution of the present invention has the property of maintaining the chlorine dioxide gas concentration in the space for a very long time when sprayed in the space.

Example 2 : Sterilization of microorganisms in a medical waste container using the chlorine dioxide solution of the present invention <Experimental method>
FIG. 3 shows an outline of the experiment of this example. The chlorine dioxide solution used in this example is a chlorine dioxide solution prepared by the same method as that described in Example 1.

  First, a medical waste container (40L) (FIG. 4) used in a hospital or the like was prepared, and a partition was provided in the center of the container (up to 2/3 of the container height). A petri dish with E. coli attached to one side of the partitioned area was placed face up (Installation A), and the same petri dish was placed face down in the other area (Installation B). 83% ethanol or the chlorine dioxide solution of the present invention was sprayed onto the Petri dish of Installation A, and 1 hour later, E. coli was collected from the Petri dish of Installation A and Installation B, and the number of viable bacteria was measured according to a conventional method. An outline of the evaluation method is shown in FIG.

<Result>
The experimental results are shown in FIG. 6 and FIG. As shown in FIG. 6 and FIG. 7, in the installation A, both 83% ethanol and the chlorine dioxide solution of the present invention were inactivated, but in the installation B (the area opposite to the sprayed area), the chlorine dioxide solution of the present invention. Only was able to inactivate E. coli. The chlorine dioxide gas concentration in the container when sprayed with the chlorine dioxide solution was 3.3 ppm. This result suggests that chlorine dioxide gas was generated from the sprayed chlorine dioxide solution, and that gas inactivated the Escherichia coli placed in the back.

  That is, according to the chlorine dioxide solution of the present invention, not only can the microorganisms at the location where the solution is directly applied be disinfected, but also the effect of maintaining the chlorine dioxide concentration in the container to which the solution is applied over a long period of time, It is possible to disinfect a portion where the liquid agent is not directly applied (for example, to a corner of a container where the liquid agent does not reach directly or to a corner of waste having a complicated structure). Moreover, according to the chlorine dioxide solution of the present invention, it is not necessary to directly apply the medical waste to the medical waste to be disinfected, but only by applying the liquid to a specific space of the container in which the medical waste is stored. Since the sterilizing effect can be exerted to every corner of the medical waste, it is possible to provide a sterilizing method that minimizes the risk of medical workers and the like coming into contact with the medical waste.

  For example, when an infectious disease with a high lethality such as a new type of influenza, Ebola hemorrhagic fever, MERS, etc. is prevalent, it is predicted that a large amount of waste that is suspected of attaching an infectious source will be generated in a short time. By applying the method of the present invention to containers for storing those wastes, it is possible to contain infection sources more simply and safely (or reduce the risk of harmful microorganisms spreading from waste containers). It becomes.

Example 3 : Inactivation of viruses in medical waste containers using the chlorine dioxide solution of the present invention <Materials and methods>
Test virus: feline calicivirus (FCV, F9, ATCC VR-782) (used as an alternative to norovirus)
Host cell: Crandell Reese felt kidney cells (CRFK, ATCC CCL-94)
Chlorine dioxide gas source: chlorine dioxide solution prepared by the same method as described in Example 1

Laboratory instruments · 1m ³ chamber (orders)
・ 96-well microplate (353072, FALCON)
・ 96-well deep well plate (BM6030, BM Bio)
・ Reagent Reservoirs / Tip-Tub (022265806, eppendorf)
・ AC FAN (MU825S-13N, ORIX)
・ Glass Petri dish (305-02, Top)
・ Cell scraper (179963, Thermo)
・ Gas detector tube (No. 23L chlorine dioxide, GASTECH)

Laboratory equipment / CO2 incubator (MCO-175AICUVH, PANASONIC)
・ Temperature and humidity meter (TR-72wf, T & D)

Experimental Materials ・ Dulbecco's Modified Eagle's Medium-high glucose (D-MEM, D5796, SIGMA)
・ Dulbecco's Phosphate Buffered Saline (D8537, SIGMA)
・ 0.25% Trypsin Solution (35555-54, Nacalai quest)
・ Fetal Bovine Serum (FBS, 30-2020, ATCC)
・ EDTA Disodium Salt 2% Solution in PBS Saline (2820549, MP)

Preparation of virus suspension:
A material obtained by diluting Feline Calicivirus that had been frozen to -80 ° C. The ⁽¹⁰ 8.5 TCID50 / 50μL) in 1% FBS solution until ¹⁰ 7-7.5 TCID50 / ^50μL was virus suspension.

Virus recovery solution (neutralization solution):
120 μL of 2% FBS D-MEM (anti- +) containing 1 mM sodium thiosulfate solution (*) was used as the virus recovery solution.
(*) 1 mM sodium thiosulfate solution has a concentration capable of neutralizing 0.1 ppmv chlorine dioxide gas without any problem.

Experimental method (see FIG. 8):
A petri dish in which 1 μl of a feline calicivirus suspension (10 ^{7 to 7.5} TCID _50/50 μL) supplemented with 1% FBS (fetal bovine serum) was dropped was placed at a position B in a 1 m ³ chamber. After the installation, the chlorine dioxide solution of the present invention was sprayed to a place about 70 cm away from the installed petri dish. The dropped FCV was exposed to and neutralized with chlorine dioxide gas generated by spraying the chlorine dioxide solution of the present invention for each time, and then the virus was recovered and the virus infectivity titer at each time was determined and evaluated. Similarly, the case where water was sprayed instead of the chlorine dioxide solution of the present invention was used as a control.

Chlorine dioxide gas concentration:
The chlorine dioxide gas concentration in the 1 m ³ chamber was measured with a detector tube.

<Result>
The experiment was performed twice.
The results of the first experiment are shown in FIG. In the first experiment, when the chlorine dioxide solution of the present invention was sprayed, the chlorine dioxide gas concentration in the chamber averaged 0.1 ppmv (min; 0.075 ppmv, max; 0.1 ppmv). In the control in which water was sprayed instead of the chlorine dioxide solution of the present invention, the virus titer after 10 minutes of spraying was 10 ^6.3 TCID _50/50 μL, but 10 ² when the chlorine dioxide solution of the present invention was sprayed. ^.8 TCID _50/50 μL (reduced by 3.5 log ₁₀ ). Further, after 15 minutes of spraying, the virus titer in the control was 10 ^4.5 TCID _50/50 μL, but when sprayed with the chlorine dioxide solution of the present invention, it became 10 ^2.3 TCID _50/50 μL ( 2.2 log ₁₀ reduction). At 30 minutes after spraying, the virus titer in the control was 10 ^5.5 TCID _50/50 μL, but when sprayed with the chlorine dioxide solution of the present invention, it was 10 ^1.0 TCID _50/50 μL (4. 5 log ₁₀ reduction).

The results of the second experiment are shown in FIG. In the second experiment, when the chlorine dioxide solution of the present invention was sprayed, the chlorine dioxide gas concentration in the chamber averaged 0.1 ppmv (min; 0.1 ppmv, max; 0.2 ppmv). In the control in which water was sprayed instead of the chlorine dioxide solution of the present invention, the virus titer after 10 minutes of spraying was 10 ^5.5 TCID _50/50 μL, but when the chlorine dioxide solution of the present invention was sprayed, the virus titer was 10 ^{0. .8} TCID _50/50 μL (4.7 log ₁₀ reduction). Also, the place for 15 minutes after spraying, the virus titer in the control was ¹⁰ 4.3 _TCID 50 / ^50μL, if a chlorine dioxide solution of the present invention were sprayed ^became 10 1.7 _TCID 50 / 50μL (2.6 log ₁₀ reduction). At 30 minutes after spraying, the virus titer in the control was 10 ^3.9 TCID _50/50 μL, but when sprayed with the chlorine dioxide solution of the present invention, it was 10 ^0.5 TCID _50/50 μL (3. 4 log ₁₀ reduction).

From the above, it was shown that the present invention can be applied not only to bacteria but also to viruses.

Claims (10)

A method for treating a medical waste container including medical waste accompanied by harmful microorganisms,
Applying a liquid agent containing chlorine dioxide gas and chlorite in the medical waste container,
The liquid agent comprises a composition that releases the chlorine dioxide gas gradually,
Method. It is a processing method of the medical waste container according to claim 1,
As the composition of the solution, the solution contains a concentration of 10 to 2000 ppm of chlorine dioxide gas, 0.05 wt% to 10 wt% of chlorite, and the pH of the solution is 4. It is prepared in the range of 5 to 6.5,
Method. It is a processing method of the medical waste container according to claim 1 or 2,
The application of the liquid agent is by spraying the liquid agent into a medical waste container or by installing the container containing the liquid agent in a medical waste container,
Method. It is a processing method of the medical waste container according to any one of claims 1 to 3,
The harmful microorganism is an infectious microorganism,
Method. It is a processing method of the medical waste container according to any one of claims 1 to 4,
The liquid agent contains 50 to 1000 ppm of chlorine dioxide gas.
Method. It is a processing method of the medical waste container according to any one of claims 1 to 5,
The solution comprises 0.1 wt% to 5.0 wt% chlorite;
Method. It is a processing method of the medical waste container according to any one of claims 1 to 6,
The pH of the solution is in the range of 5.5 to 6.0.
Method. It is a processing method of the medical waste container according to any one of claims 1 to 7,
Application of the liquid agent is characterized in that the liquid agent does not directly contact at least a part of the harmful microorganisms,
Method. A method for treating a medical waste container according to claim 8,
Application of the solution is characterized in that the solution is not in direct contact with all the harmful microorganisms,
Method. It is a processing method of the medical waste container according to any one of claims 1 to 9,
The treatment reduces the diffusion of the harmful microorganisms,
Method.