TW202216263A - Preparation method of micro-emulsified chlorophyll - Google Patents
Preparation method of micro-emulsified chlorophyll Download PDFInfo
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- TW202216263A TW202216263A TW109137623A TW109137623A TW202216263A TW 202216263 A TW202216263 A TW 202216263A TW 109137623 A TW109137623 A TW 109137623A TW 109137623 A TW109137623 A TW 109137623A TW 202216263 A TW202216263 A TW 202216263A
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- chlorophyll
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- preparing microemulsion
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- 229930002875 chlorophyll Natural products 0.000 title claims abstract description 30
- 235000019804 chlorophyll Nutrition 0.000 title claims abstract description 30
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title description 2
- 238000000034 method Methods 0.000 claims abstract description 34
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 15
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 8
- 238000004440 column chromatography Methods 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 30
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 24
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 22
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000004945 emulsification Methods 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 3
- 240000008415 Lactuca sativa Species 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 235000010445 lecithin Nutrition 0.000 claims description 3
- 239000000787 lecithin Substances 0.000 claims description 3
- 229940067606 lecithin Drugs 0.000 claims description 3
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 3
- 229940068968 polysorbate 80 Drugs 0.000 claims description 3
- 235000012045 salad Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000006837 decompression Effects 0.000 abstract 1
- 239000010410 layer Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012044 organic layer Substances 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 238000003915 air pollution Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
Description
本發明係有關於一種微乳化葉綠素備製方法，尤指一種萃取葉綠素之方式。 The present invention relates to a preparation method of micro-emulsion chlorophyll, especially a method of extracting chlorophyll.
由於環境汙染日趨嚴重且全球環保意識的抬頭，尋找替代能源一直是各國所積極持續進行之政策，尤其是針對各類可取代石油之生物能源，更是首要研究之重點之一，但大多數之生物能源在使用乃會產生大量之二氧化碳或其他有害物質，因此近年來，更致力於使用時不會產生二氧化碳之相關替代能源，其中發現了由植物中所萃取出之葉綠素，除了對於人體有相當有益之更功效且對於還經之汙染相當低之外，更可作為替代之能源，然，就目前萃取葉綠素之方法仍有相當困難之瓶頸，除了效率相當低之外，且所能夠萃取出之葉綠素純度乃有一定之限制，故，如何將上述缺失問題加以改進，乃為本案創作人所欲解決之技術困難點之所在。 Due to the increasingly serious environmental pollution and the rise of global awareness of environmental protection, the search for alternative energy has always been an active and continuous policy of various countries, especially for various types of bioenergy that can replace oil, it is one of the primary research priorities, but most of them are The use of bioenergy will produce a large amount of carbon dioxide or other harmful substances. Therefore, in recent years, more efforts have been devoted to related alternative energy sources that do not produce carbon dioxide during use. Among them, chlorophyll extracted from plants has been found. In addition to being more effective and less polluting, it can also be used as an alternative energy source. However, the current method of extracting chlorophyll still has a very difficult bottleneck. The purity of chlorophyll has certain limitations. Therefore, how to improve the above-mentioned problems is the technical difficulty that the creator of this case intends to solve.
本發明之主要目的在於：其主要包括有植物葉體前處理、超音波臭氧殺菌震盪洗淨程序、過濾分液程序、乾燥程序、管柱層析、減壓濃縮以及微乳化程序等步驟，以藉由上述步驟達到備製出微乳化葉綠素之目的。 The main purpose of the present invention is: it mainly includes the steps of plant leaf pretreatment, ultrasonic ozone sterilization and vibration cleaning procedure, filtration and liquid separation procedure, drying procedure, column chromatography, reduced pressure concentration and micro-emulsion procedure, etc. The purpose of preparing microemulsion chlorophyll is achieved by the above steps.
10:植物葉體前處理 10: Pretreatment of plant leaves
20:超音波臭氧殺菌震盪洗淨程序 20: Ultrasonic ozone sterilization shock cleaning program
30:過濾分液程序 30: Filtration and dispensing procedure
40:乾燥程序 40: Drying program
50:管柱層析 50: column chromatography
60:減壓濃縮 60: Concentration under reduced pressure
70:微乳化程序 70: Micro-emulsification procedure
第1圖係為本發明之流程圖。 Figure 1 is a flow chart of the present invention.
請參閱第1圖與所示，係為本發明之流程圖，由圖中可清楚看出，本發明萃取葉綠素之方法步驟包含如下： Please refer to Figure 1 and shown, which is a flow chart of the present invention. It can be clearly seen from the figure that the method steps of the present invention for extracting chlorophyll include the following:
步驟一：植物葉體前處理10； Step 1: Pre-treatment of plant leaves 10;
步驟二：超音波臭氧殺菌震盪洗淨程序20；
Step 2: Ultrasonic ozone sterilization and
步驟三：過濾分液程序30；
Step 3: Filtration and
步驟四：乾燥程序40；
Step 4:
步驟五：管柱層析50；
Step 5:
步驟六：減壓濃縮60；
Step 6: Concentrate under reduced
步驟七：微乳化程序70： Step 7: Micro-emulsification procedure 70:
其中，植物葉體前處理10乃是先將橘葉先進行冷凍乾燥至少24小時，並進行粉碎至可過三十目篩網之粉末狀；
Wherein, the
其中，超音波臭氧殺菌震盪洗淨程序20乃是先將上述粉末狀之橘葉加入比例為10：7：6之正己烷、丙酮與乙醇之混合液進行混合，並進行一小時之超音波震盪程序，接續再次加入正己烷並進行超音波震盪二十~三十分鐘，另外，當在進行超音波震盪時，由於過程當中會產生熱量而導致葉綠素裂解，因此乃需同時進行降溫程序；
Wherein, the ultrasonic ozone sterilization
其中，過濾分液程序30乃是先進行過濾程序，將所濾得之液體加入無水硫酸鈉水溶液，並倒入分液漏斗當中，由於分液漏斗乃具有有機層之
上層與無機層之下層等二層，因此最後葉綠素乃會儲存於上層之有機層之內，並將其取出；
Wherein, the filtration and
其中，乾燥程序40乃是將上述步驟取出之葉綠素以氮氣進行乾燥後，將其與丙酮相互混合；
Wherein, the
其中，管柱層析50乃是將上述與丙酮相互混合之葉綠素加入乙酸乙酯與甲醇之混合液，以及己烷、甲醇與丙酮之混合液來進行層析，且其中更包含下列步驟：
Wherein, the
(1)以正己烷與甲醇(9：1)之混合液進行沖提； (1) Elute with a mixture of n-hexane and methanol (9:1);
(2)以正己烷、甲醇與丙酮(89：10：1)之混合液進行沖提； (2) Elute with the mixed solution of n-hexane, methanol and acetone (89:10:1);
(3)以正己烷、甲醇與丙酮(79：20：1)之混合液進行沖提； (3) Elute with the mixed solution of n-hexane, methanol and acetone (79:20:1);
(4)以正己烷、甲醇與丙酮(75：20：5)之混合液進行沖提； (4) Elute with the mixed solution of n-hexane, methanol and acetone (75:20:5);
在經由上述混合液進行沖提後之葉綠素，則接續進行減壓濃縮60； After the chlorophyll was eluted through the above-mentioned mixed solution, the chlorophyll was successively concentrated under reduced pressure for 60;
其中，微乳化程序70乃是將上述步驟取得之葉綠素先加入沙拉油進行攪拌二十~三十分鐘，再加入Tween80(聚山梨醇酯80)進行攪拌二十~三十分鐘，再加入卵磷脂進行攪拌二十~三十分鐘，接續加入蒸餾水進行攪拌並進行超音波震盪程序一小時，最後即可獲得微乳化葉綠素；
Wherein, the
其中，之所以利用橘葉來萃取葉綠素，主要是橘子為本國產量相當大且易種植之果類，因此橘葉相當容易取得，且在產期過後，橘葉通常都是直接丟棄或以焚燒方式來處理，除了相當可惜外，更會因焚燒而造成空氣汙染。 Among them, the reason why orange leaves are used to extract chlorophyll is mainly that oranges are fruits with considerable domestic production and are easy to grow, so orange leaves are quite easy to obtain, and after the production period, orange leaves are usually discarded directly or burned. In addition to being a pity, it will also cause air pollution due to incineration.
上述所列舉的實施例係用以闡明本發明之一較佳可行實施例，並非用 以限定本發明之範圍，任何熟習此技藝者，在不脫離本發明之精神和範圍內，當可做些許更動與潤飾，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。 The above-mentioned embodiments are used to illustrate one of the preferred embodiments of the present invention, and are not intended to be used for In order to limit the scope of the present invention, any person who is familiar with this technique can make some changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be regarded as defined by the appended patent application scope. allow.
10:植物葉體前處理 10: Pretreatment of plant leaves
20:超音波臭氧殺菌震盪洗淨程序 20: Ultrasonic ozone sterilization shock cleaning program
30:過濾分液程序 30: Filtration and dispensing procedure
40:乾燥程序 40: Drying program
50:管柱層析 50: column chromatography
60:減壓濃縮 60: Concentration under reduced pressure
70:微乳化程序 70: Micro-emulsification procedure
Claims (10)
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| TW109137623A TWI744060B (en) | 2020-10-29 | 2020-10-29 | Method for preparing microemulsified chlorophyll |
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| TWI744060B TWI744060B (en) | 2021-10-21 |
| TW202216263A true TW202216263A (en) | 2022-05-01 |
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