TW202127010A - Shift detection method of absorption spectrum by the light intensity differential signals of the rising and falling edges of the absorption spectrum - Google Patents

Shift detection method of absorption spectrum by the light intensity differential signals of the rising and falling edges of the absorption spectrum Download PDF

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TW202127010A
TW202127010A TW108148651A TW108148651A TW202127010A TW 202127010 A TW202127010 A TW 202127010A TW 108148651 A TW108148651 A TW 108148651A TW 108148651 A TW108148651 A TW 108148651A TW 202127010 A TW202127010 A TW 202127010A
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detection
absorption spectrum
differential signal
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TWI716247B (en
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郭文凱
林煒薪
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國立虎尾科技大學
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Abstract

The shift detection method of absorption spectrum of the present invention includes receiving the absorption spectrum of an output light beam, wherein the absorption spectrum includes a rising edge and a falling edge; defining two detection wavelengths corresponding to the rising edge and the falling edge of the absorption spectrum; obtaining the light intensity of two detection wavelengths, wherein the two detection wavelengths correspond to the rising edge and the falling edge of the absorption spectrum; calculating the light intensity of the two detection wavelengths to obtain differential signals; and comparing the initial differential signal with the new differential signal, and determining that the absorption spectrum corresponding to the new differential signal is shifted when the new differential signal is not equal to the initial differential signal.

Description

吸收光譜偏移偵測方法Absorption spectrum shift detection method

本發明與光學感測方法有關,特別是指一種吸收光譜偏移偵測方法。The present invention is related to optical sensing methods, in particular to a method for detecting shifts in absorption spectra.

表面電漿共振(Surface Plasmon Resonance,SPR)是自由電子在金屬層與介電層產生縱向震盪的現象。當激發時,反射光能量會完全轉換成表面電漿波,此時特定波長的反射光強度趨近於零,此即為吸收光譜,反射光強度最低的波長稱為共振波長。當金屬厚度改變或介電層折射率有微小變化時,易造成共振波長的偏移,常被廣泛運用在物理、化學、生醫與生物感測器上。Surface Plasmon Resonance (SPR) is a phenomenon in which free electrons oscillate longitudinally between the metal layer and the dielectric layer. When excited, the reflected light energy will be completely converted into surface plasma waves. At this time, the reflected light intensity of a specific wavelength approaches zero, which is the absorption spectrum, and the wavelength with the lowest reflected light intensity is called the resonance wavelength. When the thickness of the metal changes or the refractive index of the dielectric layer changes slightly, it is easy to cause the shift of the resonance wavelength, which is often widely used in physics, chemistry, biomedical and biological sensors.

因為改變表面電漿共振元件的表面介質的折射率改變時會讓吸收光譜的共振波長發生偏移的現象,目前對於透過表面電漿共振系統或其他類似的感測系統輸出的吸收光譜需要透過光譜分析儀才能準確地量測,且需要耗費大量地量測時間,不利於商品化。Because changing the refractive index of the surface medium of the surface plasmon resonance element will shift the resonance wavelength of the absorption spectrum, the current absorption spectrum output through the surface plasmon resonance system or other similar sensing systems needs to be transmitted through the spectrum. The analyzer can measure accurately, and it takes a lot of measurement time, which is not conducive to commercialization.

有鑑於上述缺失,本發明的目的在於提供一種吸收光譜偏移偵測方法,其可透過偵測吸收光譜的上升緣及下降緣的光強度差動信號來偵測吸收光譜的偏移,且不需使用光譜儀,以達到簡易、低成本及快速檢測的目的。In view of the above-mentioned deficiencies, the purpose of the present invention is to provide a method for detecting absorption spectrum shift, which can detect the shift of absorption spectrum by detecting the light intensity differential signal of the rising edge and falling edge of the absorption spectrum without Need to use a spectrometer to achieve the purpose of simple, low-cost and fast detection.

為了達成上述目的,本發明的吸收光譜偏移偵測方法包括接收輸出光束的吸收光譜,吸收光譜包括上升緣及下降緣;定義對應吸收光譜的上升緣及下降緣的兩偵測波長;取得兩偵測波長的光強度,兩偵測波長對應吸收光譜的上升緣及下降緣;計算兩偵測波長的光強度以得到一差動信號;及比較初始的差動信號及新的差動信號,在新的差動信號不等於初始的差動信號時判斷新的差動信號對應的吸收光譜發生偏移。In order to achieve the above objective, the absorption spectrum shift detection method of the present invention includes receiving the absorption spectrum of the output beam, the absorption spectrum includes a rising edge and a falling edge; defining two detection wavelengths corresponding to the rising edge and the falling edge of the absorption spectrum; and obtaining two Detect the light intensity of the wavelength, the two detection wavelengths correspond to the rising edge and the falling edge of the absorption spectrum; calculate the light intensity of the two detection wavelengths to obtain a differential signal; and compare the initial differential signal and the new differential signal, When the new differential signal is not equal to the initial differential signal, it is determined that the absorption spectrum corresponding to the new differential signal has shifted.

如此,本發明的吸收光譜偏移偵測方法可以透過兩偵測波長的光強度的差動信號變化得知吸收光譜的偏移。In this way, the absorption spectrum shift detection method of the present invention can learn the shift of the absorption spectrum through the differential signal changes of the light intensity of the two detection wavelengths.

有關本發明所提供之吸收光譜偏移偵測方法的詳細環境、裝置、系統、使用或運作方式,將於後續的實施方式詳細說明中予以描述。然而,在本發明領域中具有通常知識者應能瞭解,該等詳細說明以及實施本發明所列舉的特定實施例,僅係用於說明本發明,並非用以限制本發明之專利申請範圍。The detailed environment, device, system, use, or operation method of the absorption spectrum shift detection method provided by the present invention will be described in the detailed description of the subsequent embodiments. However, those with ordinary knowledge in the field of the present invention should be able to understand that the detailed description and the specific embodiments listed for implementing the present invention are only used to illustrate the present invention, and are not intended to limit the scope of the patent application of the present invention.

以下,茲配合各圖式列舉對應之較佳實施例來對本發明的吸收光譜偏移偵測方法的技術及達成功效來作說明。然各圖式中系統的構件、組成及對應流程僅用來說明本發明的技術特徵,而非對本發明構成限制。Hereinafter, the corresponding preferred embodiments are listed in conjunction with the drawings to illustrate the technology and the achieved effects of the absorption spectrum shift detection method of the present invention. However, the components, composition, and corresponding processes of the system in the various diagrams are only used to illustrate the technical features of the present invention, but not to limit the present invention.

如圖1所示,本發明的光譜偏移偵測方法的程序10包括五個步驟,分別是接收11、定義13、取得15、計算17及比較19。接收11的步驟是接收表面電漿共振(surface plasmon resonance, SPR)感測系統輸出的輸出光束的吸收光譜,吸收光譜包括上升緣及下降緣。定義13的步驟是定義對應吸收光譜的上升緣及下降緣的兩偵測波長。取得15的步驟是取得兩偵測波長的光強度,兩偵測波長對應吸收光譜的上升緣及下降緣。計算17的步驟是計算兩偵測波長的光強度以得到差動信號。比較19的步驟是比較初始的差動信號及新的差動信號,在新的差動信號不等於初始的差動信號時判斷新的差動信號對應的吸收光譜發生偏移。As shown in FIG. 1, the procedure 10 of the spectral shift detection method of the present invention includes five steps, namely receiving 11, defining 13, obtaining 15, calculating 17, and comparing 19. The step of receiving 11 is to receive the absorption spectrum of the output beam output by the surface plasmon resonance (SPR) sensing system, and the absorption spectrum includes a rising edge and a falling edge. The step of definition 13 is to define two detection wavelengths corresponding to the rising edge and the falling edge of the absorption spectrum. The step of obtaining 15 is to obtain the light intensity of two detection wavelengths, and the two detection wavelengths correspond to the rising edge and the falling edge of the absorption spectrum. The step of calculating 17 is to calculate the light intensity of the two detection wavelengths to obtain a differential signal. The step of comparison 19 is to compare the initial differential signal with the new differential signal, and when the new differential signal is not equal to the initial differential signal, it is determined that the absorption spectrum corresponding to the new differential signal has shifted.

應用中,當表面電漿共振感測系統的被測物有變化(例如成分改變或濃度改變等)後,輸出光束的吸收光譜特性也會有對應偏移,本發明的光譜偏移偵測方法可透過動態觀察差動信號來監視吸收光譜是否發生改變。In application, when the measured object of the surface plasma resonance sensing system changes (for example, composition change or concentration change, etc.), the absorption spectrum characteristics of the output beam will also shift accordingly. The spectral shift detection method of the present invention You can monitor whether the absorption spectrum changes by dynamically observing the differential signal.

如圖2所示,接收11的步驟中,表面電漿共振感測系統30簡稱SPR感測系統,其包括光源31、第一凸透鏡32、偏振片33、第一光圈34、表面電漿共振裝置(簡稱SPR裝置)35、旋轉台36、第二光圈37及第二凸透鏡38。As shown in FIG. 2, in the step of receiving 11, the surface plasmon resonance sensing system 30 is referred to as the SPR sensing system, which includes a light source 31, a first convex lens 32, a polarizer 33, a first aperture 34, and a surface plasmon resonance device (SPR device for short) 35, a rotating table 36, a second aperture 37, and a second convex lens 38.

光源31用以產生光線,本實施例中,光源31以鹵素燈為例,但其他實施例中,光源31也可以使用LED、燈泡或其他發光元件。光線可以是單頻譜光或多頻譜光,單頻譜光例如雷射光或單色光,多頻譜光例如全頻譜光。光線依序通過第一凸透鏡32、偏振片33、第一光圈34、SPR裝置35、第二光圈37及第二凸透鏡38。光線在SPR裝置35上激發表面電漿共振。The light source 31 is used to generate light. In this embodiment, the light source 31 is a halogen lamp as an example, but in other embodiments, the light source 31 may also use an LED, a bulb, or other light-emitting elements. The light may be single-spectrum light or multi-spectrum light, single-spectrum light such as laser light or monochromatic light, and multi-spectrum light such as full-spectrum light. The light passes through the first convex lens 32, the polarizer 33, the first aperture 34, the SPR device 35, the second aperture 37, and the second convex lens 38 in sequence. The light excites surface plasmon resonance on the SPR device 35.

旋轉台37承載SPR裝置35,且可透過轉動改變SPR裝置35的角度。The rotating table 37 carries the SPR device 35, and can change the angle of the SPR device 35 through rotation.

如圖2所示,分離13的步驟是透過分光系統50,分光系統50是為了正確地定義兩偵測波長,以使兩偵測波長對應吸收光譜的上升緣及下降緣。本實施例中,分光系統50包括分光器(Beam Splitter)51、反射鏡53及帶通濾光器55。As shown in FIG. 2, the step of separating 13 is to pass through the spectroscopic system 50. The spectroscopic system 50 is to correctly define the two detection wavelengths so that the two detection wavelengths correspond to the rising edge and the falling edge of the absorption spectrum. In this embodiment, the light splitter system 50 includes a beam splitter 51, a reflector 53 and a band pass filter 55.

分光器51接收第二凸透鏡38射出的輸出光束,且將輸出光束分離成第一偵測光束及第二偵測光束。第一偵測光束是以第一入射角通過帶通濾光器55。第二偵測光束是透過反射鏡53改變入射角度後,以第二入射角通過帶通濾光器55。第一偵測光束及第二偵測光束是對應兩偵測波長(如圖4的λ1 及λ2 )的位置,因此,本實施例可透過調整帶通濾光器55的角度來調整兩偵測波長,以使兩偵測波長大致位在輸出光束的吸收光譜的上升緣及下降緣的中間,且兩者是對齊的。The beam splitter 51 receives the output light beam emitted by the second convex lens 38 and separates the output light beam into a first detection beam and a second detection beam. The first detection beam passes through the band pass filter 55 at a first incident angle. The second detection beam passes through the reflector 53 to change the incident angle, and then passes through the band-pass filter 55 at the second incident angle. The first detection beam and the second detection beam correspond to the positions of the two detection wavelengths (λ 1 and λ 2 in FIG. 4). Therefore, in this embodiment, the angle of the band-pass filter 55 can be adjusted to adjust the two wavelengths. The detection wavelength is such that the two detection wavelengths are roughly in the middle of the rising edge and the falling edge of the absorption spectrum of the output beam, and the two are aligned.

如此,中間位置可以獲得較靈敏的檢測,在輸出光束的光譜發生位移時,監視光譜的上升緣及下降緣的中間具有較佳地檢測靈敏度。In this way, the middle position can be detected more sensitively, and when the spectrum of the output beam is shifted, the middle of the rising edge and the falling edge of the monitoring spectrum has better detection sensitivity.

帶通濾光器55的濾波範圍是可以被調整的,本實施例的帶通濾光器53的濾波範圍是620 nm-700 nm,帶通寬度是13nm,其他實施例的可以選用其他能力的帶通濾光器53來調整濾波範圍及帶通寬度。The filtering range of the band-pass filter 55 can be adjusted. The filtering range of the band-pass filter 53 in this embodiment is 620 nm-700 nm, and the band-pass width is 13 nm. For other embodiments, other capabilities can be selected. The band-pass filter 53 adjusts the filtering range and the band-pass width.

其他實施例中,如圖3所示,分光系統60包括第一反射式濾光器61及第二反射式濾光器63。第一反射式濾光器61接收第二凸透鏡38射出的輸出光束,以產生第一偵測光束及一穿透光束,第一偵測光束被反射至差動處理系統70,穿透光束通過第一反射式濾光器61。第二反射式濾光器63接收穿透光束,以反射第二偵測光束至差動處理系統70。第一偵測光束及第二偵測光束分別對應兩偵測波長(如圖4的λ1 及λ2 )。如此,分光系統60也能透過第一反射式濾光器61及第二反射式濾光器63來偵測兩偵測波長的光強度。In other embodiments, as shown in FIG. 3, the light splitting system 60 includes a first reflective filter 61 and a second reflective filter 63. The first reflective filter 61 receives the output beam emitted by the second convex lens 38 to generate a first detection beam and a penetrating beam. The first detection beam is reflected to the differential processing system 70, and the penetrating beam passes through the first detection beam. A reflective filter 61. The second reflective filter 63 receives the penetrating light beam to reflect the second detecting light beam to the differential processing system 70. The first detection beam and the second detection beam respectively correspond to two detection wavelengths (λ 1 and λ 2 in FIG. 4 ). In this way, the spectroscopic system 60 can also detect the light intensity of the two detection wavelengths through the first reflective filter 61 and the second reflective filter 63.

如圖2所示,取得15、計算17及比較19的步驟是透過差動處理系統70,其接收帶通濾光器55輸出的對應第一偵測光束及第二偵測光束的兩帶通光束,並將光轉換成電信號,所以差動處理系統70也稱為光電轉換系統。透過上述說明可知,兩偵測波長大致位在吸收光譜的上升緣及下降緣的中間,且兩者是對齊的,因此,初始時兩偵測波長的光強度應是一致,透過差動處理系統70計算後初始的兩偵測波長的光強度的差動信號應為零。其他實施例中,初始的差動信號也可以不為零。As shown in FIG. 2, the steps of obtaining 15, calculating 17 and comparing 19 are through the differential processing system 70, which receives the two bandpasses corresponding to the first detection beam and the second detection beam output by the bandpass filter 55 The light beam is converted into electrical signals, so the differential processing system 70 is also called a photoelectric conversion system. From the above description, it can be seen that the two detection wavelengths are roughly in the middle of the rising edge and the falling edge of the absorption spectrum, and the two are aligned. Therefore, the light intensity of the two detection wavelengths should be the same initially, through the differential processing system 70 After the calculation, the initial differential signal of the light intensity of the two detection wavelengths should be zero. In other embodiments, the initial differential signal may not be zero.

差動處理系統70包括第一光偵測裝置71、第二光偵測裝置72、低雜訊放大裝置(或稱差動訊號放大裝置)73、資料擷取卡74及電腦75。第一光偵測裝置71及第二光偵測裝置72分別接收兩帶通光束,並調整光強度,以平衡兩帶通光束的光強度。The differential processing system 70 includes a first light detecting device 71, a second light detecting device 72, a low-noise amplifying device (or differential signal amplifying device) 73, a data capture card 74 and a computer 75. The first light detecting device 71 and the second light detecting device 72 respectively receive the two band-pass light beams and adjust the light intensity to balance the light intensities of the two band-pass light beams.

本實施例中,第一光偵測裝置71包括透鏡711、光圈713及光偵測元件715,第二光偵測裝置72包括透鏡721及光偵測元件723,其中,光圈713用以管制入射光的光強度,以平衡第一光偵測裝置71及第二光偵測裝置72的入射光的光強度,光偵測元件713、725是用以將光信號轉換為電信號。In this embodiment, the first light detecting device 71 includes a lens 711, an aperture 713, and a light detecting element 715, and the second light detecting device 72 includes a lens 721 and a light detecting element 723. The aperture 713 is used to control incident light. The light intensity of the light is used to balance the light intensity of the incident light from the first light detecting device 71 and the second light detecting device 72, and the light detecting elements 713 and 725 are used to convert the light signal into an electrical signal.

低雜訊放大裝置73是接收光偵測元件713、725輸出的電信號。資料擷取卡74依序處理電信號,並將處理後的電信號匯入電腦75中。低雜訊放大裝置73是使用Stanford Research SR560,以提供處理後的電信號給資料擷取卡74及電腦75。The low-noise amplifying device 73 receives the electrical signals output by the light detecting elements 713 and 725. The data acquisition card 74 processes the electrical signals in sequence, and imports the processed electrical signals into the computer 75. The low-noise amplifying device 73 uses Stanford Research SR560 to provide processed electrical signals to the data acquisition card 74 and the computer 75.

如圖4所示,初始的輸出光束(實線)的兩偵測波長λ1 及λ2 的光強度的差動信號應為零,圖上可明顯得到新的輸出光束(雙點鏈線)相較於輸出光束(實線)發生偏移,因此,發生偏移現象時新的輸出光束在兩偵測波長λ1 及λ2 的位置所獲得的光強度是不同的,經計算及比較後可得新的輸出光束在兩偵測波長λ1 及λ2 的差動信號與初始的差動信號不相等,偵測波長λ1 的光強度大於偵測波長λ2 的光強度,初始與新的兩偵測波長λ1 及λ2 的存在光強度差ΔR。此外,從圖上可看出光強度差ΔR的變化程度較波長變化程度Δλ更為明顯。 As shown in Figure 4, the differential signal of the light intensity of the two detection wavelengths λ 1 and λ 2 of the initial output beam (solid line) should be zero, and the new output beam (double-dot chain line) can be clearly obtained on the figure. Compared with the output beam (solid line), the light intensity obtained by the new output beam at the two detection wavelengths λ 1 and λ 2 is different when the offset occurs. After calculation and comparison, It can be obtained that the differential signal of the new output beam at the two detection wavelengths λ 1 and λ 2 is not equal to the initial differential signal. The light intensity of the detection wavelength λ 1 is greater than the light intensity of the detection wavelength λ 2 . The two detection wavelengths λ 1 and λ 2 have a light intensity difference ΔR. In addition, it can be seen from the figure that the degree of change of the light intensity difference ΔR is more obvious than the degree of wavelength change Δλ.

如圖5所示,第一偵測光束及第二偵測光束分別對準入射角30度及48度,而得到48度的吸收光譜(實線)及30度得吸收光譜(單點鏈線),並透過輸出光束的吸收光譜(粗虛線)可看出兩偵測波長大致是位在上升緣及下降緣的中間。As shown in Figure 5, the first detection beam and the second detection beam are aligned with the incident angles of 30 degrees and 48 degrees, respectively, and the absorption spectrum of 48 degrees (solid line) and the absorption spectrum of 30 degrees (single-point chain line) are obtained. ), and through the absorption spectrum (thick dashed line) of the output beam, it can be seen that the two detection wavelengths are roughly in the middle of the rising edge and the falling edge.

本發明的吸收光譜偏移偵測方法只需要偵測波長的光強度差動變化即可獲得吸收光譜偏移狀態,因此,本發明可以達成快速檢測的目的。The absorption spectrum shift detection method of the present invention only needs to detect the light intensity differential change of the wavelength to obtain the absorption spectrum shift state. Therefore, the present invention can achieve the purpose of rapid detection.

再者,透過差動處理系統來消除兩帶通光譜的共通雜訊(common noise)來獲得更好的檢測能力,共通雜訊例如光源浮動與背景光的雜訊等,使本發明對環境雜訊有更好的免疫性,因為光學檢測的過程中環境雜訊容易影響實驗結果,因此,本發明可以達到即時監控數據並消除雜訊,使靈敏度與解析度得到良好的提升。Furthermore, the common noise of the two bandpass spectra is eliminated through the differential processing system to obtain better detection capabilities. Because the environmental noise in the optical detection process easily affects the experimental results, the present invention can achieve real-time monitoring of data and eliminate noise, so that the sensitivity and resolution are improved.

實驗中,依次在SPR裝置內增加水的蔗糖濃度,以在三個不同的時間點將液體的折射率從0%更改為0.179%,0.304%和0.396%。圖6是差分處理系統的電壓相對時間的信號圖,圖7是差分處理系統中電壓相對折射率的信號圖。圖中顯示,對於折射率小於1.3336可獲得良好的線性度,系統的靈敏度為1429 V / RIU。 從圖6中的局部放大5000倍圖可以發現本發明能將雜訊限制(壓低)在0.01 V(0.865V-0.875V)的雜訊峰值。In the experiment, the sucrose concentration of water was sequentially increased in the SPR device to change the refractive index of the liquid from 0% to 0.179%, 0.304%, and 0.396% at three different time points. FIG. 6 is a signal diagram of voltage versus time in a differential processing system, and FIG. 7 is a signal diagram of voltage versus refractive index in a differential processing system. The figure shows that good linearity can be obtained for a refractive index less than 1.3336, and the sensitivity of the system is 1429 V / RIU. It can be found from the partially enlarged 5000 times map in FIG. 6 that the present invention can limit (slow down) the noise to a noise peak of 0.01 V (0.865V-0.875V).

最後,再次強調,本發明於前揭實施例中所揭露的構成元件,僅為舉例說明,並非用來限制本案之範圍,其他等效元件的替代或變化,亦應為本案之申請專利範圍所涵蓋。Finally, it is emphasized again that the constituent elements disclosed in the previously disclosed embodiments of the present invention are only examples and are not used to limit the scope of the case. The substitution or changes of other equivalent elements shall also be subject to the scope of the patent application of this case. Covered.

10:程序 11:接收 13:定義 15:取得 17:計算 19:比較 30:表面電漿共振感測系統 31:光源 32:第一凸透鏡 33:偏振片 34:第一光圈 35:表面電漿共振裝置 36:旋轉台 37:第二光圈 38:第二凸透鏡 50:分光系統 51:分光器 53:反射鏡 55:帶通濾光器 60:分光系統 61:第一反射式濾光器 63:第二反射式濾光器 70:差動處理系統 71:第一光偵測裝置 711:透鏡 713光圈 715:光偵測元件 72:第二光偵測裝置 721:透鏡 723:光偵測元件 73:低雜訊放大裝置 74:資料擷取卡 75:電腦 λ1 、λ2 :偵測波長10: Program 11: Receive 13: Definition 15: Acquire 17: Calculation 19: Comparison 30: Surface Plasma Resonance Sensing System 31: Light Source 32: First Convex Lens 33: Polarizer 34: First Aperture 35: Surface Plasma Resonance Device 36: rotating table 37: second aperture 38: second convex lens 50: spectroscopic system 51: spectroscope 53: reflector 55: band-pass filter 60: spectroscopic system 61: first reflective filter 63: first Second reflection filter 70: differential processing system 71: first light detecting device 711: lens 713 aperture 715: light detecting element 72: second light detecting device 721: lens 723: light detecting element 73: Low noise amplifier 74: data capture card 75: computer λ 1 , λ 2 : detection wavelength

圖1是本發明的吸收光譜偏移偵測方法的步驟流程圖。 圖2是應用本發明吸收光譜偏移偵測方法的實施例的環境的示意圖。 圖3是應用本發明吸收光譜偏移偵測方法的實施例的另一環境的示意圖。 圖4是初始的輸出光束及新的輸出光束的光譜圖。 圖5是初始的輸出光束、第一偵測光束及第二偵測光束的吸收光譜的光譜圖。 圖6是圖2中差分處理系統產生的電壓-時間信號圖。 圖7是圖2中差分處理系統產生的電壓-折射率的信號圖。FIG. 1 is a flow chart of the steps of the method for detecting the shift of the absorption spectrum of the present invention. FIG. 2 is a schematic diagram of an environment in which an embodiment of the absorption spectrum shift detection method of the present invention is applied. FIG. 3 is a schematic diagram of another environment in which an embodiment of the absorption spectrum shift detection method of the present invention is applied. Figure 4 is a spectrum diagram of the original output beam and the new output beam. FIG. 5 is a spectrum diagram of the absorption spectra of the initial output beam, the first detection beam, and the second detection beam. Fig. 6 is a voltage-time signal diagram generated by the differential processing system in Fig. 2. FIG. 7 is a signal diagram of the voltage-refractive index generated by the differential processing system in FIG. 2.

10:程序10: Procedure

11:接收11: receive

13:定義13: Definition

15:取得15: Obtain

17:計算17: calculation

19:比較19: Comparison

Claims (9)

吸收光譜偏移偵測方法,包括下列步驟: 接收一輸出光束的一吸收光譜,該吸收光譜包括一上升緣及一下降緣; 定義對應該吸收光譜的該上升緣及該下降緣的兩偵測波長; 取得該兩偵測波長的光強度,該兩偵測波長對應該吸收光譜的該上升緣及該下降緣; 計算該兩偵測波長的光強度以得到一差動信號;及 比較初始的該差動信號及新的該差動信號,在新的該差動信號不等於初始的該差動信號時判斷新的該差動信號對應的該吸收光譜發生偏移。The absorption spectrum shift detection method includes the following steps: Receiving an absorption spectrum of an output beam, the absorption spectrum including a rising edge and a falling edge; Define the two detection wavelengths corresponding to the rising edge and the falling edge of the absorption spectrum; Obtain the light intensity of the two detection wavelengths, the two detection wavelengths corresponding to the rising edge and the falling edge of the absorption spectrum; Calculate the light intensity of the two detection wavelengths to obtain a differential signal; and The initial differential signal and the new differential signal are compared, and when the new differential signal is not equal to the initial differential signal, it is determined that the absorption spectrum corresponding to the new differential signal has shifted. 如申請專利範圍第1項所述的吸收光譜偏移偵測方法,其中,該輸出光束是透過一表面電漿共振感測系統產生。According to the method for detecting the shift of the absorption spectrum described in the scope of the patent application, the output beam is generated through a surface plasmon resonance sensing system. 如申請專利範圍第1項所述的吸收光譜偏移偵測方法,其中,該定義包括一分光系統來決定該兩偵測波長。The method for detecting the shift of the absorption spectrum as described in claim 1, wherein the definition includes a spectroscopic system to determine the two detection wavelengths. 如申請專利範圍第3項所述的吸收光譜偏移偵測方法,其中,該分光系統包括一分光器、一反射鏡及一帶通濾光器,該分光器接收該輸出光束以產生一第一偵測光束及一第二偵測光束,該反射鏡反射該第二偵測光束,該帶通濾光器接收該第一偵測光束及該第二偵測光束,該第一偵測光束及該第二偵測光束是對應該兩偵測波長。According to the method for detecting the shift of the absorption spectrum described in the scope of patent application, wherein the light splitting system includes a beam splitter, a reflector and a band pass filter, and the beam splitter receives the output light beam to generate a first A detection beam and a second detection beam, the mirror reflects the second detection beam, the band-pass filter receives the first detection beam and the second detection beam, the first detection beam and The second detection beam corresponds to two detection wavelengths. 如申請專利範圍第4項所述的吸收光譜偏移偵測方法,其中,該取得、該計算及該比較包括一差動處理系統,接收及處理該第一偵測光束及該第二偵測光束以獲得對應的光強度。Such as the absorption spectrum shift detection method described in claim 4, wherein the acquisition, the calculation and the comparison include a differential processing system that receives and processes the first detection beam and the second detection Light beam to obtain the corresponding light intensity. 如申請專利範圍第3項所述的吸收光譜偏移偵測方法,其中,該分光系統包括一第一反射式濾光器及一第二反射式濾光器,該第一反射式濾光器接收該輸出光束以產生一第一偵測光束及一穿透光束,該第二反射式濾光器接收該穿透光束以產生一第二偵測光束,該第一偵測光束及該第二偵測光束是對應該兩偵測波長。The absorption spectrum shift detection method according to the third item of the scope of patent application, wherein the spectroscopic system includes a first reflective filter and a second reflective filter, and the first reflective filter The output beam is received to generate a first detection beam and a penetrating beam, the second reflective filter receives the penetrating beam to generate a second detection beam, the first detection beam and the second The detection beam corresponds to two detection wavelengths. 如申請專利範圍第6項所述的吸收光譜偏移偵測方法,其中,該取得、該計算及該比較包括一差動處理系統,接收及處理該第一偵測光束及該第二偵測光束以獲得對應的光強度。Such as the absorption spectrum shift detection method described in item 6 of the scope of patent application, wherein the acquisition, the calculation and the comparison include a differential processing system that receives and processes the first detection beam and the second detection Light beam to obtain the corresponding light intensity. 如申請專利範圍第1項所述的吸收光譜偏移偵測方法,其中,初始的該差動信號等於零。According to the method for detecting the shift of the absorption spectrum described in the first item of the scope of the patent application, the initial differential signal is equal to zero. 如申請專利範圍第1項或第8項所述的吸收光譜偏移偵測方法,其中,該兩偵測波長是對應該吸收光譜的該上升緣及該下降緣的中間。The method for detecting the shift of the absorption spectrum as described in item 1 or item 8 of the scope of patent application, wherein the two detection wavelengths correspond to the middle of the rising edge and the falling edge of the absorption spectrum.
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