TW202123871A - A methode for measuring height of skin basal cell - Google Patents

Abstract

The invention is related to a methode for measuring the height of a skin basal cell, comprising the following steps: providing a non-invasive microscopy device for shooting a picture of at least one skin basal cell of skin to be tested, including a laser source for emitting laser light with pulsed laser and an image processing element for processing image signals; converging the laser light to the skin to be tested via the image processing element and obtaining 2D images; utilizing the image processing element to stack the 2D images; labelling the first appearing 2D image of the at least one basal cell as a first layer; labelling the just disappearing 2D image of the at least one basal cell as a last layer; and multiplying the stacking number of the stacking images between the first layer and the last layer with a preset distance between two stacking images via calculation, thereby obtaining the height of the at least one basal cell for assessing of a skin pigmentation patient to be cured.

Description

A method for measuring the height of skin basal cells and

The present invention relates to a method for measuring the height of skin basal cells, by comparing the height of basal cells with skin diseases and normal cells to obtain identification and analysis of pathological characteristics.

Pigment disorders, including sunburn, zygomatic mother spots, etc., are skin diseases with extremely high prevalence, which usually occur on the face and hands. For older white women, the prevalence rate of sunburn on the face is as high as 90%, and for older Asian women, the prevalence rate is as high as 70%. The most common treatment is laser spot removal, but after a certain period of time after the spot removal is completed, almost all patients undergoing the operation will relapse. Because the pigmentation of the face is not life-threatening, the pathological study of this disease is incomplete, and there is no pathological evaluation of the efficacy of laser facial treatment to understand the cause of recurrence after surgery. Facing the huge medical and beauty market demand, it is urgent to provide high-penetration, high-resolution, and quantitative microscopic pathological images under the skin without removing tissues and causing no facial skin damage. of.

The present invention aims to advance the frequency-doubled microscopy developed in the past and provide a method for image analysis of the height of skin basal cells, so as to have more flexible orientation operation, image resolution and stability, Synchronize and integrate three-dimensional facial recognition and diagnosis between doctors' consultations, and become a customized image processing environment that provides quantitative pathological information such as melanin molecules, skin cells, and tissues.

Another object of the present invention is to provide standards compatible with medical digital image transmission protocols. Through dermatological clinical trials, this method should be applied to the identification and analysis of the pathological characteristics of sunburn and zygomatic mother spots, and long-term tracking of mines The changes in the lesions after radiotherapy are evaluated by pathological evaluation of the curative effect before and after treatment.

Another object of the present invention is to provide an advanced method of in vivo optical virtual sectioning, which can perform comprehensive non-invasive scanning and long-term monitoring of the diseased area in clinic, and help to improve the classification of clinical diseases and provide real-time diagnosis guidelines. Decide and engage in the pathological evaluation of the curative effect before and after the urgently needed treatment.

With the image analysis method of the basal cell height of the present invention, and the statistical results of clinical experiments, it is found that the basal cell height in the sunburn area is higher than that of normal skin, which has a statistically significant difference. And after the laser spot removal operation, the height of the basal cells in the sunburn area decreases, which can represent a better treatment effect. Generally speaking, according to intuition, it is believed that the closer to the normal skin state, the better the treatment effect.

The method for measuring the height of skin basal cells according to the present invention includes: providing a non-invasive microscope device for photographing at least one skin basal cell of the skin to be tested, including: a device for emitting laser light with pulsed laser A laser light source, and an image processing component for processing image signals; the laser light is collected on each of the at least one skin basal cell of the skin to be tested through the image processing component, and a two-dimensional image is obtained by using the image processing component Image; using the image processing component to image each of the two-dimensional images in a stack; marking the two-dimensional stacked image of each of the at least one basal cell that has just begun to appear as the first layer; image of each of the at least one basal cell that has just disappeared Mark Mark it as the last layer; and calculate the stacking number of the two-dimensional stacked image between the first layer and the last layer of each of the at least one basal cell, and multiply it by a preset distance between the two stacked images to Obtain the height of the at least one basal cell for treatment evaluation of skin pigmentation disorders, wherein the two-dimensional stacked image of each of the skin basal cells is along the optical axis of the non-invasive microscope device from the continuity of the at least one skin basal cell The two-dimensional stacked image of the from the skin epidermis to the skin dermis direction, that is, the surface (enface) direction, arbitrarily select a basal cell for height analysis, and wherein each of the at least one layer appears between each of the first layer and the last layer Hollow in the basal cell nucleus.

100,200‧‧‧Non-invasive microscopy device

110,210‧‧‧laser light source

120，230‧‧‧Image processing component

130，240‧‧‧Basal cells

220‧‧‧Regulating component

Fig. 1 shows a schematic diagram of the measured basal cells standing upright in the method for measuring the height of skin basal cells according to the present invention.

Fig. 2 shows a schematic diagram of the measured basal cells being tilted in the method for measuring the height of skin basal cells according to the present invention.

Fig. 3(a) shows a block diagram of an embodiment of a non-invasive microscope device used in the method for measuring the height of skin basal cells according to the present invention.

FIG. 3(b) shows a block diagram of another embodiment of a non-invasive microscope device used in the method for measuring the height of skin basal cells according to the present invention.

Figure 4 shows an image of skin basal cells obtained by frequency-doubled microscopy of the present invention.

According to the skin basal analysis method of the present invention, the schematic diagram of the erect basal cells shown in FIG. 1 and the schematic diagram of the inclined basal cells shown in FIG. A schematic diagram of the images of different depths of the enface direction. The image of the basal cells just appearing is the two-dimensional image of (1), and the image of the basal cells just disappearing is the two-dimensional image of (3), and in between (2) A cavity in the basal cell nucleus will appear, so the distance between these two two-dimensional images is called "basal cell height".

3(a) and 3(b) respectively show the block diagrams of different embodiments of the method for measuring skin basal cells according to the present invention using a non-invasive microscopy device.

Referring to FIGS. 1, 2 and 3, a method for measuring the height of skin basal cells of the present invention includes: providing a non-invasive microscope device 100 for photographing at least one skin basal cell of the skin to be tested, as shown in FIG. 3 As shown, it includes: a laser light source 110 for emitting laser light with pulsed laser, and an image processing component 120 for processing image signals; the laser light is concentrated on the under-test through the image processing component Each of the at least one skin basal cell 130 of the skin, and using the image processing component to obtain a two-dimensional image; using the image processing component to stack each of the two-dimensional images; The two-dimensional image (as shown in (1) of FIG. 1 and FIG. 2) is marked as the first layer (also the layer (b) of the embodiment shown in FIG. 4); each image of at least one basal cell just disappeared Marked as the last layer (as shown in Figure 1 and Figure 2(3) and Figure 4(f)); and: Refer to Figure 4 for the image of skin basal cells obtained by frequency-doubled microscopy Calculate the stacking number of the two-dimensional stacked image between the first layer and the last layer of each of the at least one basal cell (ie, the (b) to (f) layers shown in the embodiment of FIG. 4, and there are 4 layers in between ), multiply it by a preset distance between two stacked images or adjacent images (the distance between the two stacked images or adjacent images is the same, in one embodiment, the distance is set Is 1.8 microns) to obtain the height of the at least one basal cell (1.8 (micron) x 4 = 7.2 (micron) in this example) for the evaluation of the treatment of skin pigment disorders, wherein two of the skin basal cells The three-dimensional stacked image is along the non-invasive In the optical axis of the type microscope device, the continuous two-dimensional stacked image of the at least one skin basal cell is from the skin epidermis to the skin dermis direction, that is, the en face direction. Any basal cell is selected for height analysis, In addition, a cavity of each of the at least one basal cell nucleus appears between the first layer and the last layer (as shown in (2) of FIG. 1 and FIG. 2).

In the method for measuring the height of skin basal cells of the present invention, the non-invasive microscope device 100 used in conjunction with it can be a reflection type confocal microscope (RCM), two-photon excitation fluorescence microscope (TPEF), and frequency doubled microscope. (HGM), or Optical Coordination Tomography (OCT).

In addition, as shown in FIG. 3, in the method for measuring the height of skin basal cells of the present invention, a non-invasive microscopy device 200 of another different embodiment includes a laser light source 210, an image processing component 230, and The basal cell 240 shoots an image, which may further include a regulating member 220 disposed between the laser light source 210 and the image processing member 230 for regulating the pulse width, intensity and path of the laser light, including: a double chirp Chirped mirror, a first lens group, a table subtractor, a light guide arm, a mirror, a two-dimensional graphic scanner, a second lens group, a beam splitter and a three-axis micro-control platform, and the The image processing component includes a convex lens, a beam splitter, two filters, two photomultiplier tubes and a computer. The images taken by the non-invasive microscope device of this structure can be double-frequency and/or triple-frequency images.

In the method for measuring the height of skin basal cells of the present invention, one embodiment is: the maximum diameter of the at least one basal cell in the enface direction is about 10 microns, and the entire length and width of each image are greater than 10 microns, The entire image must cover at least one basal cell. In addition, the lateral resolution of the two-dimensional non-invasive microscope device is less than 5 microns. In addition, the height of the basal cells is about 20 microns, and the Z-axis resolution of the non-invasive microscope device is less than 10 microns.

In addition, the method used in the method for measuring the height of skin basal cells of the present invention The Z-axis spacing of the non-invasive microscope device is less than 10 microns. Furthermore, in the method for measuring the height of skin basal cells of the present invention, the at least one basal cell is plural, and the height of the basal cell is the average value of the plural basal cells. In one embodiment, the plurality of basal cells are distributed in a nine-square grid.

Claims (10)

A method for measuring the height of skin basal cells, including: A non-invasive microscope device for photographing at least one skin basal cell of the skin to be tested is provided, including: a laser light source for emitting laser light with pulsed laser, and an image processing component for processing image signals ； Converging the laser light on each of the at least one skin basal cell of the skin to be tested through the image processing component, and obtaining a two-dimensional image by using the image processing component; Using the image processing component to stack and image each of the two-dimensional images; Marking the two-dimensional image of each of the at least one basal cell that has just appeared as the first layer; Mark each image in which at least one basal cell has just disappeared as the last layer; and Calculate the stacking number of the two-dimensional stacked images between the first layer and the last layer of each of the at least one basal cell, and multiply it by a preset distance between two stacked images to obtain the at least one basal cell Height for the evaluation of the treatment of skin pigment disorders, The two-dimensional stacked image of each of the skin basal cells is along the optical axis of the non-invasive microscope device, and the continuous two-dimensional stacked image from the at least one skin basal cell is from the skin epidermis to the skin dermis direction, that is, the surface (enface ) Direction, arbitrarily select a basal cell for height analysis, and a cavity of each of the at least one basal cell nucleus appears between the first layer and the last layer. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the non-invasive microscope device is a reflective confocal microscope (RCM), two-photon excitation fluorescence microscope (TPEF), frequency doubled microscope (HGM) , Or Optical Coordination Tomography (OCT). For example, the method for measuring the height of skin basal cells in the first item of the scope of patent application further includes an adjustment The control component is arranged between the laser light source and the image processing component to control the pulse width, intensity and path of the laser light, and includes: a pair of chirped mirrors, a first lens group, a table subtractor, A light guide arm, a mirror, a two-dimensional graphic scanner, a second lens group, a beam splitter, and a three-axis micro-control platform, and the image processing component includes a convex lens, a beam splitter, and two filters Optical film, two photomultiplier tubes and a computer. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the maximum diameter of the at least one basal cell in the enface direction is about 10 microns. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the entire length and width of each image are greater than 10 microns, and the entire image must cover at least one basal cell. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the lateral resolution of the two-dimensional non-invasive microscope device is less than 5 microns. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the height of the basal cells is about 20 microns, and the Z-axis resolution of the non-invasive microscope device is less than 10 microns. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the Z axis interval of the non-invasive microscope device is less than 10 microns. For example, the method for measuring the height of skin basal cells in the scope of the patent application, wherein the at least one basal cell is plural, and the height of the basal cell is the average value of the plural basal cells. For example, the method for measuring the height of skin basal cells in item 9 of the scope of patent application, wherein the plurality of basal cells are distributed in a nine-square grid.

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