TW202116290A - Use of sea berry (hippophaerhamnoides) extract for improving skin condition - Google Patents

Abstract

A use ofHippophae rhamnoides extract is for preparing a composition. The composition has one or more of the following functions for improving skin condition: moisturizing skin, tightening skin pores, smoothening skin wrinkles and texture, and relieving skin redness.

Description

Sea buckthorn (Sea 　 Berry) extract for skin conditioning purposes

The present invention relates to products related to sea buckthorn, in particular to the use of sea buckthorn extracts to prepare skin conditioning compositions.

Since the rise of the concept of organic and natural diets, biotech companies and food companies have actively invested in the research and development of products related to natural plants. In order to make plant-related products have a basis for scientific verification of health benefits, plant active ingredient analysis and efficacy evaluation have become key items in product development.

Hippophae rhamnoides (Hippophae rhamnoides), also known as sea buckthorn, is a plant of the genus Hippophae rhamnoides in the family Elaeagnus. Seabuckthorn is a hardy deciduous shrub with spines native to Asia and Europe, and its fruit is orange-yellow. Sea buckthorn can prevent wind and fix sand, and the fruit of sea buckthorn has high medicinal value. Therefore, sea buckthorn has also become one of the targets of active research and development by biotechnology companies and food companies.

In view of this, the present invention provides a use of sea buckthorn extract, which is a composition for preparing skin conditioning. Among them, sea buckthorn extract has one or more of the following abilities: increasing skin moisture content, tightening skin pores, smoothing skin wrinkles and texture, and soothing skin redness.

In one embodiment, a use of sea buckthorn extract is to increase the moisture content of the skin.

In some embodiments, a sea buckthorn extract is used to tighten skin pores.

In one embodiment, a use of sea buckthorn extract is to smooth wrinkles and texture of the skin.

In some embodiments, sea buckthorn extract is used to soothe the redness of the skin.

In some embodiments, the aforementioned composition is a pharmaceutical product.

In some embodiments, the aforementioned composition is a food product or food additive.

In some embodiments, the aforementioned composition is a cosmetic or skin care product.

Regarding the concentration symbol "wt%" used in this article usually refers to weight percent concentration, and the concentration symbol "vol%" usually refers to volume percent concentration.

The "significant difference" between the two data mentioned in this article or the diagram in this case refers to the statistically significant difference between the two data, which is statistically analyzed by the student's t-test. The "*" in the figure represents the value before using the sea buckthorn extract in this case and the value after use, the p value is less than 0.05, and the "**" in the figure represents the value before and after using the sea buckthorn extract in this case In comparison with the values, the p-value is less than 0.01. In addition, the "#" in the figure represents the data after using the placebo and the data after using the sea buckthorn extract in this case. When the two are compared, the p value is less than 0.05, and the "##" in the figure represents the placebo. Comparing the latter data with the data after using the seabuckthorn extract in this case, the p-value is less than 0.01.

In some embodiments, the sea buckthorn extract obtained from the fruit of sea buckthorn (Hippophae rhamnoides ) may have at least one of the following abilities: increasing skin moisture content, tightening skin pores, smoothing skin wrinkles and texture, and soothing skin redness. Therefore, the sea buckthorn extract can be used to prepare a composition having at least one of the following abilities: enhancing skin moisture content, tightening skin pores, smoothing skin wrinkles and texture, and soothing skin redness.

In some embodiments, the aforementioned composition may be a pharmaceutical product. In other words, this medicine contains an effective amount of sea buckthorn extract. Among them, the medicine can be manufactured into a dosage form suitable for intestinal, parenterally, orally, or topically, using techniques well known to those skilled in the art.

In some embodiments, the dosage form for enteral or oral administration may be, but is not limited to, a tablet, a troche, a lozenge, a pill, or a capsule. , Dispersible powder or granule, solution, suspension, emulsion, syrup, elixir, slurry or similar. In some embodiments, the dosage form for parenteral or topical administration may be, but is not limited to, injection, sterile powder, external preparation, or the like. In some embodiments, the injection method may be subcutaneous injection, intraepidermal injection, intradermal injection, or intralesional injection.

In some embodiments, the medicine containing sea buckthorn extract may further include a pharmaceutically acceptable carrier that is widely used in pharmaceutical manufacturing technology. In some embodiments, the pharmaceutically acceptable carrier may be one or more of the following carriers: solvent, buffer, emulsifier, suspending agent, decomposing agent ( decomposer, disintegrating agent, dispersing agent, binding agent, excipient, stabilizing agent, chelating agent, diluent, glue Gelling agent, preservative, wetting agent, lubricant, absorption delaying agent, liposome and the like. Regarding the type and quantity of the selected carrier, it falls within the scope of professionalism and routine technology of those who are familiar with this technology. Among them, the solvent as a pharmaceutically acceptable carrier may be water, normal saline (normal saline), phosphate buffered saline (PBS), or alcohol containing aqueous solution.

In some embodiments, the aforementioned composition is an edible composition. In other words, the edible composition contains a specific content of sea buckthorn extract. Among them, the form of the edible composition can be powder, granule, solution, colloid or paste.

In some embodiments, the edible composition containing sea buckthorn extract may be a food product or a food additive.

In some embodiments, the food product containing sea buckthorn extract may be beverages, fermented foods, bakery products, health foods, or dietary supplements, etc. . In some embodiments, the food product containing sea buckthorn extract may further include an adjuvant. For example, the adjuvant may be Maltodextrin, malic acid, sucralose, citric acid, fruit flavor, honey flavor, steviol glycoside or a combination thereof, and the like. Regarding the type and quantity of the selected carrier, it falls within the scope of professionalism and routine technology of those who are familiar with this technology.

In some embodiments, the food additives containing sea buckthorn extract may be seasonings, sweeteners, spices, pH adjusters, emulsifiers, colorants or stabilizers, etc.

In some embodiments, the aforementioned composition is a cosmetic or skin care product. In other words, cosmetics or skin care products contain a specific content of sea buckthorn extract.

In some embodiments, cosmetics or skin care products containing sea buckthorn extract can be any of the following types: lotion, gel, jelly film, mud mask, lotion, cream, lipstick, foundation, pressed powder, powder, makeup remover Oil, makeup remover, facial cleanser, body wash, shampoo, hair conditioner, sunscreen, hand cream, nail polish, perfume, essence and facial mask.

In some embodiments, the cosmetics or skin care products containing sea buckthorn extract may further contain externally acceptable ingredients as needed. In some embodiments, the external product acceptable ingredient may be, for example, an emulsifier, a penetration enhancer, a softener, a solvent, an excipient, an antioxidant, or a combination thereof.

In some embodiments, the sea buckthorn extract can be obtained by performing an extraction procedure with a suitable extraction solvent and sea buckthorn fruits.

For example, in the extraction procedure, sea buckthorn fruits are extracted with water at 50° C.-100° C. for 30 minutes to 90 minutes for an appropriate time to obtain the initial extraction stock solution. Among them, the seabuckthorn fruit to be extracted can be a whole fruit, or a fruit separated into fragments, granules or powder after physical pretreatment. The physical pretreatment used may include at least one of the following: shredding, shearing, mashing, and grinding. In some embodiments, in the extraction procedure, the initial extraction stock solution may be further filtered to remove impurities to obtain a filtrate. In some embodiments, in the extraction procedure, the filtrate may be further concentrated to obtain a concentrated liquid. Among them, the concentration method can be concentrated under reduced pressure. In some embodiments, during the extraction procedure, the concentrate can be further dried into a powder. Among them, the drying method can be spray drying.

That is to say, the primary extraction liquid, filtrate, concentrated liquid or powder obtained from the extraction procedure can be used as the sea buckthorn extract according to actual needs.

In some embodiments, the sea buckthorn extract may also be a raw juice, a concentrated raw juice or a dried powder of a commercially available sea buckthorn fruit.

[Example 1]: Vitamin C quantification

Weigh 10mg of Vitamin C (L-Ascorbic Acid Sodium Salt, purity ≧98%, purchased from SIGMA-ALORICH) in a quantitative bottle, then _{dissolve it with pure water (H 2} O) and quantify to 10 mL to obtain the initial solution (That is, it contains 1000ppm of vitamin C). Then, configure the standard solutions of 10 ppm, 25 ppm, 50 ppm, 100 ppm and 200 ppm according to the following table 1.

Table I Standard solution (ppm) 10 25 50 100 200 Initial solution (mL) 0.1 0.25 0.5 1.0 2.0 Pure water (mL) 9.9 9.75 9.5 9.0 8.0 Total amount (mL) 10 10 10 10 10

Weigh 1.0 g of sea buckthorn juice powder (which contains 100% sea buckthorn extract) (purchased from Puredia) and place it in a quantitative bottle, then _{dissolve it with pure water (H 2} O) and quantify it to 50 mL to obtain the solution to be tested.

Then, the test solution and the standard solution were injected into a high performance liquid chromatograph (HPLC) (model: Hitachi Chromaster HPLC system, brand: Hitachi, Tokyo, Japan) for liquid chromatography, and the test solution was combined with Comparison and identification of the retention time and absorption spectrum of the peak obtained from the standard solution. Among them, HPLC is equipped with Ascentis Express C18 chromatography column (brand: Supelco, inner diameter 2.1mm, length 100mm, particle size 2μm). In the HPLC settings, the mobile phase is 0.1wt% oxalic acid aqueous solution, the flow rate is 0.2 ml per minute (0.2 mL/min), the injection volume is 2 μL, the number of injections is 3 repetitions, the detection wavelength is 245 nm, and the column The temperature is set to 30°C.

Then, the content of vitamin C is calculated according to the following formula (1).

Vitamin C content (ppm)=C×V÷M (1)

Among them, C is the concentration (ppm) of vitamin C in the solution to be tested obtained from the standard curve. V is the final quantified volume of the solution to be tested, that is, 50 mL. M is the weight of the sample to be sampled and analyzed, that is, 1g.

Here, the residence time of vitamin C is 1.8 min (minutes). In addition, it was determined that the sea buckthorn juice powder had 9334.03 ppm of vitamin C.

[Example 2]: Instant human body experiment-using sea buckthorn extract for external use

Samples used: a mask containing sea buckthorn extract essence (made by soaking a mask cloth in the essence containing sea buckthorn extract to make it fully absorbed) and a placebo mask (using the same type of mask cloth soaked in the aforementioned essence In the liquid, but the sea buckthorn in it is replaced with water of equal weight), and then it is made after it is fully absorbed. In this embodiment, each facial mask contains 20 mL of the corresponding essence.

Essence ingredients containing sea buckthorn extract: Sanxian gum 0.2wt%, Cinxantone 0.6wt%, 1,3-butanediol 5wt%, triethanolamine 0.1wt%, hexylene glycol 0.6wt%, sea buckthorn extract 0.5 wt%, the rest is water. Among them, the sea buckthorn extract is prepared from sea buckthorn juice with sea buckthorn juice powder (which contains 100% sea buckthorn extract) (purchased from Puredia) with pure water to form a solution with a degree of brix (Degrees Brix) of 5.6.

Number of subjects: 6 subjects aged 20-50.

Experimental method: After the subjects have cleaned their entire face, according to different test items, use corresponding instruments and measurement methods to record the value of the left and right half of the face before using the mask, or take a photo before use. Then, a mask containing sea buckthorn extract essence and a placebo mask were applied to the right half of the face and the left half of the subject. After 15 minutes, they were removed, and then the left half of the face and the left half of the face and the left half of the face were slightly massaged with the fingertips. The right half of the face. After the mask is removed for about 5 minutes, use the corresponding instrument and measurement method to measure or take a photo, and compare it with the value of the original left or right face (when you want to test before and after use) , The temperature and humidity of the test area where the subject is located are consistent, in order to reduce the influence of external temperature and humidity on the skin).

Test items: 1. Skin moisture content, 2. Skin pores, and 3. Skin redness index will be performed on the skin respectively.

1. Skin moisture content

The skin moisture detection probe Corneometer ^® CM825 (C+K Multi Probe Adapter System, Germany) purchased from Courage+Khazaka electronic, Germany was used to detect the facial skin of the same subject before and after applying the mask. The detection probe is based on the principle of capacitance for measurement. When the moisture content changes, the capacitance value of the skin also changes, so the skin capacitance value can be measured to analyze the moisture content of the skin surface.

Please refer to Figure 1. This picture shows the change in skin moisture content of the subjects before using the mask and placebo mask containing the essence of sea buckthorn extract and after 15 minutes of use (based on the skin moisture content before using the mask, calculated as a percentage) ). It can be seen from the graph that the skin moisture content increased by 12.6% on average, and the improvement rate reached 100% of the subjects. At the same time, there was a significant difference in the moisture content of the skin after the subjects used the mask containing the essence of sea buckthorn extract compared with the placebo mask (p value less than 0.01). It can be seen that the sea buckthorn extract of the present invention has the purpose of increasing the moisture content of the skin.

2. The state of skin pores

The VISIA high-end digital skin quality tester sold by Canfield in the United States was used for testing. The facial skin of the same subject before and after the mask was tested through a high-resolution camera lens. By standard white light irradiation, shadows are formed in the depressions of the pores, and the pores will be darker than the surrounding skin tone, so the pores can be detected. Then, use the software to analyze the number and area of pores to obtain a value, and the higher the value, the larger the number and area of pores.

Please refer to Figure 2. This picture shows the change in the number of skin pores before and after 15 minutes of using the mask and placebo mask containing the essence of sea buckthorn extract (based on the number of skin pores before using the mask, calculated as a percentage) ). It can be seen from Figure 2 that after the subjects used the mask containing the essence of sea buckthorn extract, their skin pores were reduced by an average of 11.4%, and the improvement rate reached 83.3% of the subjects. It can be seen that the seabuckthorn extract of the present invention has the purpose of tightening skin pores.

3. Skin red pigment index

The skin redness index detection probe Mexameter® MX18 (C+K Multi Probe Adapter System, Germany) from Courage+Khazaka electronic, Germany was used to detect the facial skin of the same subject before and after applying the mask. The instrument determines the amount of hemoglobin in the skin by measuring the amount of reflection of light of a specific wavelength on the human skin. The higher the measured value, the higher the red pigment content in the skin.

Please refer to Figure 3. This graph shows the changes in the skin red pigment of subjects before using the mask containing the sea buckthorn extract and placebo mask and 15 minutes after using the skin red pigment (based on the skin red pigment before using the mask, calculated as a percentage) ). It can be seen from the figure that the redness of the skin is reduced by an average of 13.9%, and the proportion of people who have improved has reached 100% of the subjects. It can be seen that the seabuckthorn extract of the present invention has the functions of reducing the red pigment of the skin and soothing the redness of the skin.

[Example 3]: Long-acting human experiment-using sea buckthorn extract for external use

Samples used: a mask containing sea buckthorn extract essence (made by soaking a mask cloth in the essence containing sea buckthorn extract to make it fully absorbed) and a placebo mask (using the same type of mask cloth soaked in the aforementioned essence In the liquid, but the sea buckthorn in it is replaced with water of equal weight), and then it is made after it is fully absorbed. In this embodiment, each facial mask contains 20 mL of the corresponding essence.

Essence ingredients containing sea buckthorn extract: Sanxian gum 0.2wt%, Cinxantone 0.6wt%, 1,3-butanediol 5wt%, triethanolamine 0.1wt%, hexylene glycol 0.6wt%, sea buckthorn extract 0.5 wt%, the rest is water. Among them, the sea buckthorn extract is prepared from sea buckthorn juice with sea buckthorn juice powder (which contains 100% sea buckthorn extract) (purchased from Puredia) with pure water to form a solution with a degree of brix (Degrees Brix) of 5.6.

Number of subjects: 7 subjects aged 20-50.

Experimental method:

(1) Data measurement before using the mask: clean the subject's entire face, use the corresponding instrument and measurement method according to different test items, record the value of the left and right half of the face before using the mask, or take a photo before use .

(2) Use of facial mask: The subjects applied the mask containing the essence of sea buckthorn extract and the placebo mask on their right and left sides respectively. After 15 minutes, they took them off, and then put them on their fingertips. Slightly massage the left half of the face and the right half of the face. The aforementioned steps are carried out in the morning and evening every day for two weeks.

(3) Data measurement after use: After the subject has used the mask for two weeks, use the corresponding instrument and measurement method to measure or take a photo, and then compare it with the value of the original left or right half of the face ( When testing before and after use, the temperature and humidity of the test area where the subject is located are the same to reduce the influence of external temperature and humidity on the skin).

Test items: 1. Skin moisture content, 2. Skin wrinkles, and 3. Skin texture will be performed on the skin respectively.

1. Skin moisture content

The skin moisture detection probe Corneometer ^® CM825 (C+K Multi Probe Adapter System, Germany) purchased from Courage+Khazaka electronic, Germany was used to detect the facial skin of the same subject before and after applying the mask. The detection probe is based on the principle of capacitance for measurement. When the moisture content changes, the capacitance value of the skin also changes, so the skin capacitance value can be measured to analyze the moisture content of the skin surface.

Please refer to Figure 4. This picture shows the change in skin moisture content of the subjects before using the mask and placebo mask containing the essence of sea buckthorn extract in this case and after two weeks of daily use (based on the skin moisture content before using the mask, based on Percentage calculation). It can be seen from the figure that the skin moisture content increased by 16.7% on average (significant difference between before and after using the essence mask containing sea buckthorn extract, p value is less than 0.01), and the proportion of people who improved reached 100% of the subjects. At the same time, there was a significant difference in the moisture content of the skin after the subjects used the mask containing the essence of sea buckthorn extract compared with the placebo mask (p value less than 0.05). It can be seen that the seabuckthorn extract of the present invention not only has the instantaneous effect of increasing the moisture content of the skin, but also has the purpose of increasing the moisture content of the skin for a long time.

2. Skin wrinkles

Use the VISIA high-end digital skin quality tester sold by Canfield in the United States to detect the same subject's facial skin before and after using the mask through a high-resolution camera lens. The skin is detected by standard white light irradiation When the shadow changes, the texture position can be detected and a value can be obtained, which can represent the smoothness of the skin.

Refer to Figure 5. This graph shows the changes in skin wrinkles between subjects before using the mask and placebo mask containing the essence of sea buckthorn extract and after two weeks of daily use (based on the wrinkle status of the skin before using the mask, as a percentage Calculation). It can be seen from the figure that skin wrinkles are reduced by an average of 14.2% (significant difference between before and after using the essence mask containing sea buckthorn extract, p value is less than 0.05), and the improvement rate reaches 100% of the subjects. It can be seen that the sea buckthorn extract of the present invention has long-lasting use in smoothing wrinkles.

3. Skin texture

The VISIA high-end digital skin quality tester sold by Canfield in the United States was used to test the facial skin of the same subject before and after the mask. The principle is to shoot high-resolution skin images with visible light and use the built-in software The roughness of the pits and bumps of the skin is analyzed. The higher the measurement value, the rougher the skin.

Refer to Figure 6. This graph shows the change in skin texture of subjects before using the mask and placebo mask containing the essence of sea buckthorn extract and after two weeks of daily use (based on the skin texture state before using the mask, in percentage Calculation). It can be seen from the figure that the skin texture is reduced by 11.7% on average (significant difference between before and after using the essence mask containing sea buckthorn extract, p-value is less than 0.05), and the improvement rate reaches 100% of the subjects. It can be seen that the sea buckthorn extract of the present invention has a long-term effect of increasing the moisture content of the skin.

In summary, the use of the seabuckthorn extract according to any embodiment of the present invention can be used to prepare a composition for conditioning the skin condition. In other words, the aforementioned composition has one or more of the following functions: enhancing skin moisture content, enhancing skin moisture content, tightening skin pores, smoothing skin wrinkles and texture, and soothing skin redness.

no

Figure 1 shows the change in skin moisture content of subjects before using a mask containing seabuckthorn extract and a placebo mask, and 15 minutes after use (based on the skin moisture content before using the mask, based on Percentage calculation). Figure 2 shows the changes in the number of skin pores before and after 15 minutes of using the mask and placebo mask containing the essence of sea buckthorn extract (based on the number of skin pores before using the mask) Percentage calculation). Figure 3 shows the changes in the skin red pigment of subjects before using the mask containing the sea buckthorn extract and placebo mask, and after 15 minutes of use (based on the skin red pigment before using the mask, calculated as a percentage ). Figure 4 shows the changes in the skin moisture content of the subjects before using the mask and placebo mask containing the essence of sea buckthorn extract and after two weeks of daily use (based on the skin moisture content before using the mask, based on Percentage calculation). Figure 5 shows the changes in skin wrinkles of subjects before using the mask and placebo mask containing the essence of sea buckthorn extract and after two weeks of daily use (based on the state of skin wrinkles before using the mask, Calculated as a percentage). Figure 6 shows the changes in the skin texture of subjects before using the mask containing the sea buckthorn extract essence and placebo mask, and after two weeks of daily use (based on the skin texture state before using the mask, Calculated as a percentage).

Claims (10)

A use of a sea buckthorn extract for preparing a skin conditioning composition, wherein the vitamin C content of the sea buckthorn extract is greater than 9000 ppm. The use according to claim 1, wherein the composition comprises at least 1% wt of the sea buckthorn extract. The use according to claim 1, wherein the sea buckthorn extract is used to improve skin moisture retention. The use according to claim 1, wherein the sea buckthorn extract is used to reduce or prevent the generation of skin pores. The use according to claim 1, wherein the sea buckthorn extract is used to soothe skin redness. The use according to claim 1, wherein the sea buckthorn extract is used to smooth skin wrinkles or texture. The use according to any one of claims 1 to 6, wherein the composition is a medicine. The use according to any one of claims 1 to 6, wherein the composition is a food product or a food additive. The use according to any one of claims 1 to 6, wherein the composition is a cosmetic or skin care product. The use according to claim 9, wherein the frequency of use of the cosmetic or skin care product is at least once a day.

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