TW202019493A - Biofilm removal system - Google Patents

Abstract

The present invention discloses a biofilm removal system for removing a biofilm in a cavity. The biofilm removal system includes a vehicle and a removal device. The vehicle includes a magnetic nanobead and a treatment substance. The removal device includes a magnetism control component and a vibration component. The magnetism control component is configured to guide the magnetic nanobead to drive the vehicle close to the biofilm. The vibrating component is configured to generate vibrations and focus the vibrations around the biofilm to create microbubbles to strip at least a portion of the biofilm from the cavity and destroy the vehicle to release the treatment substance. The present invention uses the vibration component to strip off most of the biofilm, and at the same time, the vehicle releases treatment substance to inhibit the remaining bacteria, thereby achieving high-efticiency treatment.

Description

Biofilm removal system

A system for removing biofilms, especially a system for removing biofilms in a cavity by using vibration and magnetic force.

With the advancement of medicine, more and more pipes and artificial implants are used in medical treatment and improving human life. However, while obtaining the benefits of these medical aids, the problem of infection is an inevitable and difficult problem. In particular, bacterial infections often cause complications and sequelae, and even become severe enough to cause sepsis. Therefore, the prevention and treatment of bacterial infections is the direction of current medical research. In particular, the chance of urinary tract infections accounts for about 40% of medical-related infections. And when the time that the urinary catheter is left in the body increases by 1 day, the incidence of bacteriuria increases by 3% to 10%. Therefore, effective removal of bacterial infections of the bladder is a subject that must be improved at present.

To solve the problem of bacterial infection, the formation of biofilms must be solved first, because the formation of biofilms is a survival strategy for microorganisms. Biofilm is a structure formed by microorganisms and extracellular substances secreted by microorganisms attached to the surface of the object. In other words, biofilm is a community formed by a pile of microorganisms. The main purpose of growing in groups is to resist the pressure caused by the external environment, such as: nutritional deficiency, pH value, extreme temperature, or even the threat of detergents or antibiotics.

For the removal of biofilms formed in the human body, the current medical methods are mostly the physical treatment of vortexing, scraping, rinsing, scrubbing biofilms or using antibiotics or Biochemical treatment of medicaments. However, the consensus for antibiotic treatment is that the lower the dose, the better, if necessary, to reduce the risk of bacteria developing resistance. More importantly, it is very easy to damage normal tissues and cells when using conventional methods for biofilm removal. Therefore, there is an urgent need for a technology that can implement precision medicine and inhibit biofilm formation.

In view of this, the present invention discloses a biofilm removal system, which is applied to remove biofilm on an inner cavity wall of a cavity. The cavity also contains liquid. The biofilm removal system includes a carrier and a removal device. The carrier is located in the cavity and contains a nano-magnetic bead, a bacteriostatic substance for specifically inhibiting the biofilm, and a thermosensitive lipid film covering the bacteriostatic substance and the nano-magnetic bead. The cleaning device is located outside the cavity and contains a magnetic control component and a vibration component. The magnetic control component is used to guide the nano magnetic beads to drive the carrier to approach the biofilm. The vibrating component is used to generate a plurality of vibrations and focus the vibration to a limited distance of the biofilm or biofilm outside, so as to generate micro-bubbles in the liquid to separate at least a part of the biofilm from the inner cavity wall and destroy the thermal sensitivity Lipid film to release bacteriostatic substances.

The vibration assembly further includes a plurality of vibration elements. The vibration elements are arranged to form a phased array. Each vibrating element generates vibration in sequence and the energy of the vibration reaches the biofilm or a limited distance outside the biofilm at the same time.

The vibration component further focuses the vibration into the cavity to cause vortex or turbulence of the liquid, and at least a part of the biofilm is detached from the inner cavity wall by the vortex or the turbulence.

The micro-bubbles generated by the vibrating component in the cavity act upon the biofilm when the pressure wave is eliminated, so that at least a part of the biofilm is separated from the inner cavity wall.

The frequency of vibration generated by the vibrating component is between 0.3MHz and 4.0MHz.

The magnetron component further generates a high-frequency alternating magnetic field to generate thermal energy to inhibit the biofilm and destroy the thermosensitive lipid film to release bacteriostatic substances.

The cleaning device further includes a first probe. The first probe includes the aforementioned magnetic control component, vibration component and a magnetic shield. The magnetic shielding is located between the magnetic control component and the vibration component to reduce the electromagnetic effect caused by the magnetic control component on the vibration component.

The carrier is roughly circular, and the diameter of the carrier is less than 0.22 μm.

The carrier further includes a biofilm ligand. The first end of the biofilm ligand is joined to the outside of the thermosensitive lipid membrane, and the second end of the biofilm ligand is used to join to the biofilm.

The biofilm removal system further includes a second probe for receiving echo. The echo is generated by the vibration generated by the vibrating component or a second vibration generated by a second vibration source touching the cavity and the biofilm.

The biofilm removal system of the present invention provides multiple removal methods, which can be performed separately or simultaneously to obtain different effects.

One of the methods for removing the biofilm in the cavity is to inject the carrier into the cavity; the nanomagnetic beads of the magnetron assembly are used to guide the nanomagnetic beads to drive the carrier close to the biofilm; the vibration component is controlled to generate a plurality of vibrations, and these vibrations are focused Within a limited distance to the biofilm or one of the outside of the biofilm; use these vibrations to focus to generate a plurality of micro-bubbles that contact the biofilm, so that at least a part of the biofilm is separated from the inner cavity wall; at the same time, the vibration energy or the thermal energy generated by the vibration destroy The heat-sensitive lipid film releases bacteriostatic substances. Since the carrier is close to the biofilm, the bacteriostatic substances will also smoothly affect the bacteria in the biofilm.

Another method to remove the biofilm in the cavity is to inject the carrier into the cavity; use the biofilm ligand on the carrier to specifically bind to the biofilm; control the production of the magnetron assembly The high-frequency AC magnetic field causes the nano-magnetic beads in the carrier to generate magnetic vibration; the magnetic vibration generates heat energy higher than the body temperature to kill bacteria in the biofilm; the heat-sensitive lipid film of the carrier is due to the magnetic energy and the thermal energy generated by the magnetic vibration. Be destroyed and release the antibacterial substance inside.

Another method for removing the biofilm in the cavity includes the following steps: close the vibration component to the corresponding outer surface of the cavity; control the vibration component to generate a plurality of vibrations, and focus these vibrations on the biofilm; then, use these vibrations to focus and generate A plurality of micro-bubbles in contact with the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall.

Compared with the conventional technology, the method for removing the biofilm in the cavity of the present invention can remove the biofilm in the organism. The mechanism of using a vibration device with a phased array probe to peel off the biofilm can remove the biofilm deeper into the inner cavity wall than the surgical method of physical debridement. The method of the invention using a vibrating component with a phased array probe to peel off the biofilm does not harm the body tissue of the patient more than the current cleaning method. The invention uses the carrier and the nano-magnetic beads to carry the drug to the biofilm site, so that the drug can more accurately exert the effect. In this way, the dosage of the medicine can be reduced, avoiding tissue damage and reducing the burden of the body's metabolic medicine. The invention can be carried out directly from the body of the patient who placed the catheter, and the removed biofilm is discharged from the body through the catheter, or the device can be entered into the patient's bladder along the catheter, making it easier to grasp the focus position of the vibration.

In addition, the vibrating component is used to strip most of the obvious biofilm, but it cannot remove all the bacteria in the space. After the operation, the bacteria will still clump quickly, forming biofilm again. On the other hand, various types of drugs (such as antibiotics) cannot easily pass through the protection of the biofilm, and even if the maximum dose is increased, the bacteria in the biofilm cannot be completely removed. The system of the present invention further combines the advantages of complementing the two, using a vibrating component to strip most of the visible biofilm, while The carrier releases the agent, and most of the peeled biofilm can be removed by flowing out of the cavity in other ways. The bacteria or free bacteria remaining in the small amount of biofilm remaining in the cavity will be destroyed by the drug. In this way, the effect of the present invention in removing biofilms is far superior to existing biofilm cleaning technologies.

1‧‧‧ Biofilm removal system

2‧‧‧Clear equipment

3‧‧‧Carrier

4‧‧‧Biofilm

5‧‧‧cavity

21‧‧‧ First probe

23‧‧‧Magnetic control components

24‧‧‧Magnetic shielding

25‧‧‧Vibration components

30‧‧‧thermal grease film

32‧‧‧Bacteriostatic substance

37‧‧‧ Biofilm ligand

38‧‧‧Nano magnetic beads

50‧‧‧Inner cavity wall

51‧‧‧Liquid

251‧‧‧Vibration element

FIG. 1 is a schematic diagram of a cavity and a biofilm in a specific embodiment of the invention.

2 is a schematic diagram of a carrier in a specific embodiment of the present invention.

FIG. 3 is a schematic diagram of a cleaning device in a specific embodiment of the present invention.

4 is a schematic diagram of a biofilm removal system in a specific embodiment of the invention.

FIG. 5 is a schematic diagram of a cleaning device in another specific embodiment of the present invention.

6 is a schematic diagram of a carrier in another embodiment of the present invention.

In order to make the advantages, spirit and features of the present invention easier and clearer to understand, detailed description and discussion will follow with embodiments and reference to the accompanying drawings. It is worth noting that these examples are only representative examples of the present invention. However, it can be implemented in many different forms and is not limited to the embodiments described in this specification. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

The terminology used in the various embodiments disclosed in the present invention is only for the purpose of describing specific embodiments, and is not intended to limit the various embodiments disclosed in the present invention. The singular form system as used herein also includes the plural form unless the context clearly indicates otherwise. Unless otherwise defined, all terms (including technical and scientific terms) used in this specification have the same meanings as those generally understood by those of ordinary skill in the art to which various embodiments of the present disclosure belong. The above terms (such as those defined in a commonly used dictionary) will be interpreted as having the same meaning as the contextual meaning in the same technical field, and will not be interpreted as having an idealized meaning or an excessively formal meaning Unless clearly defined in the various embodiments disclosed in the present invention.

In the description of this specification, the description with reference to the terms "one embodiment", "a specific embodiment", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment are included in at least one embodiment of the present invention . In this specification, the schematic expression of the above terms does not necessarily refer to the same embodiment. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments.

Please refer to Figure 1 to Figure 4. FIG. 1 is a schematic diagram of a cavity 5 and a biofilm 4 in a specific embodiment of the invention. FIG. 2 is a schematic diagram of the carrier 3 in a specific embodiment of the present invention. FIG. 3 is a schematic diagram of the cleaning device 2 in a specific embodiment of the present invention. 4 is a schematic diagram of a biofilm removal system 1 in a specific embodiment of the invention. The invention discloses a biofilm removal system 1 which is applied to remove the biofilm 4 on an inner cavity wall 50 in a cavity 5. A liquid 51 is also contained in the cavity 5. The biofilm removal system 1 includes a carrier 3 and a removal device 2. The carrier 3 is located in the cavity 5 and includes a nano-magnetic bead 38, a bacteriostatic substance 32 for specifically inhibiting the biofilm 4, and a thermosensitive lipid film 30 covering the bacteriostatic substance 32 and the nano-magnetic bead 38 . The cleaning device 2 includes a magnetron assembly 23 and a vibration assembly 25. The magnetron assembly 23 is used to guide the nanomagnetic beads 38 to drive the carrier 3 to approach the biofilm 4. The vibrating component 25 is used to generate a plurality of vibrations and focus the vibrations to a limited distance between the biofilm 4 and the biofilm 4 to generate micro bubbles in the liquid 51 so that at least a part of the biofilm 4 is separated from the inner cavity wall 50, and destroy the thermosensitive lipid film 30 to release the bacteriostatic substance 32.

In the present invention, two principles are mainly used to cooperatively remove the biofilm 4, one is physical removal, and the energy of the cavity effect is used to peel the biofilm 4 off the inner cavity wall 50. Available later The opening of the cavity 5 naturally discharges the peeled biofilm. The other is biochemical removal, which uses a magnetic field to drive the carrier 3 to the biofilm 4 and then release the antibacterial substance 32 therein. These two removal methods have their own advantages and disadvantages. Physical removal is difficult to eliminate bacteria in tiny biofilms that are difficult to detect, while biochemical removal is difficult to poison bacteria in a wide range of biofilms. However, the present invention can perform two types of removal methods in the same treatment course, and both can use the vibration generated by the vibration component 25 to achieve removal, making the removal of biofilms more efficient than traditional methods.

The cavity 5 may refer to the bladder, urethra, or other organs containing liquid and high humidity, and may also be a medical device for containing liquid, a medical device for transferring liquid, etc., but is not limited thereto. The biofilm 4 refers to a substrate produced by the breeding of bacteria, which allows bacteria to produce colonies therein. The heat-sensitive lipid film 30 is formed by heat-sensitive nano liposomes. The bacteriostatic substance 32 can achieve a universal or specific inhibitory effect on different types of bacteria, thereby inhibiting the formation or expansion of biofilms, or directly killing bacteria. For example, antibiotics against E. coli, radioactive substances, antibodies against antigens, compounds that generate heat energy when released from the carrier 3, vaccines against certain viruses or certain bacteria, alcohols, bactericides or disinfectants, etc. The effect of the compound.

Among the elements contained in the carrier 3, the bacteriostatic substance 32 can also be replaced by an environment improving substance. In principle, the environment-improving substance does not directly inhibit bacteria, but it can change the environment in the cavity, so that the cavity immunity is enhanced, bacteria are not easy to grow, or biofilms are not easy to form. The environment-improving substance may be a kind of beneficial bacteria, which is less harmful to the organism, but the beneficial bacteria will change the environment of the cavity in which it is located. Such an environment is not suitable for the growth of some strains, and thus repels harmful bacteria. The environment-improving substance may be an acidic compound or a basic compound. When the compound is released, it will change the pH value in the cavity, so that the bacteria will die below this pH value, or it is not easy to be in this pH value. Inside the cavity. When the cavity system is an organ of an organism, the environment-improving substance may be a nutrient to make the organ obtain sufficient nutrition, so that the organ is sound and naturally improves immunity; when the organ or tissue is damaged due to bacterial infection, the environment The improving substance may be a bioremediation factor that improves organ tissues and restores them to their original immunity.

In the present invention, the environment-improving substance and the bacteriostatic substance 32 can be reasonably replaced with each other or be contained in the carrier 3 at the same time.

The cavity 5 is not a closed structure, or, at least in part, it is not a closed structure, so that the carrier 3 enters the cavity 5 and the biofilm 4 that is easy to peel off leaves the cavity 5. The present invention can be applied to remove biofilms in the excretory system of living organisms. In this case, the cavity 5 may be a bladder or a urethra. The carrier 3 is formed outside the cavity 5 and can enter the cavity 5 by introduction, administration, injection, infusion, or the like. In a specific embodiment, the cavity 5 is a bladder, and the carrier 3 is injected into the bladder through a catheter that has been installed on the patient. The removal device 2 does not need to invade the interior of the cavity 5, and the removal device 2 is medically defined as a non-invasive treatment device, and the peeled-off biofilm 4 is then discharged out of the body through a urinary catheter. In still another embodiment, the removal device 2 can also enter the interior of the cavity 5 through an existing tube, for example, into the bladder through a urinary catheter, so that it is easier to control the focus position of the vibration during the operation.

The method for removing the biofilm in the cavity of the present invention can remove the biofilm in the organism, or the biofilm in the medical equipment. The mechanism of using a vibrating component to peel off the biofilm is more capable of removing the biofilm that has penetrated into the inner cavity wall than the surgical method of physical debridement. The method of using the vibrating component to peel off the biofilm in the present invention does not damage the patient's body tissues more than the current cleaning methods. The invention utilizes the carrier and the nano-magnetic beads to carry the antibacterial substance to the position of the biofilm, so that the antibacterial substance can exert the effect more accurately. By this method Can reduce the dose of bacteriostatic substances, thereby reducing the body's burden of metabolizing bacteriostatic substances, and also reduce the degree of bacterial resistance. The invention can directly allow the removal device to enter the bladder along the patient's urinary catheter for removal, or directly from the outside of the body, and the removed biofilm can be discharged out of the body through the catheter.

The vibration assembly 25 further includes a plurality of vibration elements 251. The vibration elements 251 are arranged to form a phased array. Each vibrating element 251 generates vibration in sequence and the energy of the vibration reaches the biofilm 4 or within a limited distance outside the biofilm 4 at the same time. The limited distance is 30mm. By limiting the distance, an effective operating range can be roughly divided. The maximum distance from the effective operating range to the biofilm 4 is the defined distance.

In a specific embodiment, the vibration component 25 is an ultrasonic vibration component, and the ultrasonic vibration component has a phased array of ultrasonic components.

For practical applications, the vibrating component 25 can utilize high-intensity focused ultrasound technology to achieve that energy reaches the desired location at the same time. The principle is that the distance and angle of each vibration element 251 and the target position are different, but the transmission speed of the vibration wave is the same. When the vibrating element 251 far away from the target position first generates vibration, and the vibrating element 251 closer to the target position generates vibration within a very short time, two vibrational energy will reach the target position at the same time, making the target position instantaneous Receive high energy to produce subsequent physical phenomena. The advantage of using this technique is that the material between the vibrating component 25 and the target position will not receive instantaneous high energy and have a severe impact. When this technology is used to remove the biofilm in the living body, the tissues and organs between or around the biofilm and the vibration component 25 can be prevented from being injured. On the other hand, it can reduce the energy absorbed by tissues and organs, and there is enough energy to reach the biofilm at the target location. When the energy is focused in the biofilm 4, the vibration energy generates heat energy in the biofilm, which can exceed 40 degrees, destroying the physiology or structure of the bacteria in the biofilm, thereby causing the death of the bacteria. Or again In another embodiment, the vibration energy affects the chromosome of the bacteria, causing the chromosome to break and causing the bacteria to die.

In a specific embodiment, the present invention utilizes vibration focusing to generate microbubbles that contact the biofilm, and the pressure wave generated when the microbubbles are destroyed acts on the biofilm so that at least a portion of the biofilm is detached from the inner cavity wall. Further, when the liquid in the lumen and the extracellular material of the biofilm are focused by vibration, microbubbles will be generated on at least one of the surface of the biofilm, the inside of the biofilm, and the wall between the lumen and the biofilm. Therefore, the pressure wave generated when the bubbles are extinguished will act on at least one of the surface of the biofilm, the interior of the biofilm, and the wall between the lumen and the biofilm, thereby generating between the biofilm or between the biofilm and the wall of the lumen Gap.

In a specific embodiment, the vibration component 25 is disposed on the phased array probe. Each vibration element is sequentially excited according to the distance between each vibration element and the target object, so that each vibration element generates vibration in sequence, and the energy of each vibration reaches the biofilm at the same time. Further, the technology used by the phased array probe is to control each vibrating element to vibrate at different times, and these vibrations will be transmitted in the form of waves. Taking the position of the target object vertically corresponding to the phased array probe as the center point, the vibrating element of the phased array probe starts to vibrate in sequence from the vibrating element farther from the center point to the closer vibrating element, and at the same distance from the center point The vibrating element will vibrate at the same time. When the waves generated by each vibrating element reach the interference point at the same time, these waves will produce constructive interference, and then focus on the last interference point after the constructive interference. In the present invention, the interference point is set on the biofilm, so that the biofilm is detached due to the micro bubbles generated by the vibration. The above-mentioned way of focusing vibration can prevent other unfocused tissues from being injured. When the energy is focused on the operating range around the biofilm 4, the vibration energy causes cavitation in the cavity and generates microbubbles, which impact the biofilm 4 And wiped out. The pressure wave generated when the micro-bubbles are destroyed acts on the biofilm 4, so that at least a part of the biofilm 4 is separated from the inner cavity wall 50. In another specific embodiment, the vibration energy emitted is adjusted so that the vibration is focused into the cavity 5 to cause a vortex or turbulence of the liquid 51, and at least a part of the biofilm 4 is detached from the biofilm 4 by the vortex or the turbulence内腔墙50。 The lumen wall 50.

The frequency of the vibration generated by the vibration component 25 is between 0.3 MHz and 4.0 MHz. Specifically, the generated frequency may fall within 0.3 MHz, 0.5 MHz, 0.8 MHz, 1.0 MHz, 1.2 MHz, 2.0 MHz, 3.0 MHz, 4.0 MHz, or a value between the above frequencies.

In addition to guiding the magnetic beads close to the biofilm 4, the magnetron assembly 23 can further generate a high-frequency alternating magnetic field to generate thermal energy to inhibit the biofilm 4 and destroy the thermosensitive lipid film 30 to release the bacteriostatic substance 32. Both the high-frequency AC magnetic field and the high-frequency vibration have the effect of destroying the heat-sensitive lipid film 30, and when they act on the heat-sensitive lipid film 30 at the same time, they have a multiplication effect, and quickly release more antibacterial substances 32 in the heat-sensitive lipid film 30 .

Due to the principle of surface tension, the carrier 3 is substantially circular. The diameter of the carrier 3 is less than 0.22 μm, and it can enter the organism by injection or oral administration. With the above-mentioned precise removal method, the total amount of the medicament used each time can be far less than the traditional method of removing the biofilm using only the medicament, and achieve the same removal effect. The precise, low-concentration agent can prevent other tissues or cells of the organism from being injured.

Please refer to Figure 4 and Figure 6. 6 is a schematic diagram of a carrier in another embodiment of the present invention. The carrier 3 further includes a biofilm ligand 37. The first end of the biofilm ligand 37 is joined to the outside of the thermosensitive lipid membrane 30, and the second end of the biofilm ligand 37 is used to join to the biofilm 4. When the carrier 3 of the magnetron assembly is close to the biofilm 4, the biofilm ligand 37 will be adsorbed on the biofilm. When the magnetron assembly 23 of the cleaning device 2 emits high-frequency interactive magnetic field energy, the nanomagnetic beads 38 vibrate and Generate heat to kill bacteria in the biofilm; when the cleaning device 2 emits energy to destroy the carrier 3, the inhibitor released Bacterial material can effectively contact the biofilm.

See Figure 5. FIG. 5 is a schematic diagram of a cleaning device in another specific embodiment of the present invention. In a specific embodiment, the cleaning device 2 further includes a first probe 21. The first probe 21 includes the aforementioned magnetron assembly 23, the vibration assembly 25 and a magnetic shield 24. The magnetic shield 24 is located between the magnetic control component 23 and the vibration component 25 to reduce the electromagnetic effect caused by the magnetic control component 23 on the vibration component 25. In this embodiment, the stability of the vibration component 25 can be maintained by the magnetic shielding 24. In addition, the magnetron assembly 23 and the vibration assembly 25 are concentrated on the first probe 21, which facilitates one-handed operation.

In another specific embodiment, the cleaning device further includes a third probe (not shown), the third probe is provided with a magnetron component, and the first probe is provided with a vibration component. Under some conditions, high-frequency vibration and magnetic fields must transfer energy from different angles of the cavity. In this case, the magnetron and vibration components need to be located in different probes, which is conducive to precision medical treatment.

The biofilm removal system 1 further includes a second probe (not shown). The second probe has a detection component for receiving echoes, or can emit vibrations. The echo system is caused by the vibration of the vibration component 25, the vibration of the detection component, or The vibration generated by a third vibration source touches the cavity and the biofilm. Through the detection of the detection component, the precise position of the biofilm can be searched out, and then the first probe 21 is used to clear the biofilm. In a specific embodiment, the detection component may be located on the first probe.

In terms of application, the detection component, the magnetron component, and the vibration component can be set on three different probes, or any two of them can be on the same probe, or all three can be on the same probe, depending on the case or Practical needs.

In one category of the present invention, a biofilm removal system is further provided for removing a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes a Cleaning equipment, cleaning equipment contains detection components and vibration components. The detection component is used to generate a first vibration, so that the cavity generates an echo corresponding to the first vibration, and the detection component further receives the echo. The vibration component further includes a plurality of vibration elements forming a phased array, each vibration element sequentially generates a second vibration and the energy of the second vibrations reaches the biofilm and its range at the same time, so that the biofilm and its range A heat energy is generated inside, and the temperature of the biofilm is higher than the temperature of the inner cavity wall by the heat energy. In a specific embodiment, the cavity system is the bladder, and the temperature of the inner cavity wall is the biological body temperature. The heat energy generated in this way makes the temperature of the biofilm higher than the temperature of the bladder wall, and most of the bacteria will die at a higher temperature, thus achieving the effect of killing the bacteria in the biofilm.

In another category of the present invention, a biofilm removal system is further provided for removing a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes a carrier and a removal device. The carrier is located in the cavity and includes a nano-magnetic bead and an outer membrane for covering the nano-magnetic bead. The cleaning device includes a vibrating component and a magnetic control component. The magnetron assembly is used to guide the carrier to or around the biofilm. The vibrating component is used to generate a vibration to break the outer membrane and release the nano magnetic beads. Then, the magnetron component generates a high-frequency alternating magnetic field to generate thermal energy by the magnetic vibration nano magnetic beads, and the thermal energy increases the temperature of the biofilm, thereby killing bacteria that are not resistant to high temperature inside the biofilm.

The biofilm removal system of the present invention provides multiple removal methods. Performing the following removal methods separately can obtain different effects; but the following methods can also be combined with each other in a reasonable way to achieve more diverse application methods.

A method for removing the biofilm in the cavity is applied to remove the biofilm attached to the inner cavity wall of the target. The method includes the following steps: providing a cleaning device containing a first probe, the first probe includes a vibrating component and a magnetron component; inputting a carrier into the target. The carrier contains bacteriostatic substances and nano magnetic beads; the first probe is close to the corresponding outer surface of the target; the magnetic control component is controlled to make the carrier close to the biofilm: the vibration component is controlled to generate a plurality of vibrations, and these vibrations are focused to the biological On the membrane; use these vibrations to focus to generate a plurality of micro-bubbles that contact the biological membrane, so that at least a part of the biological membrane is separated from the inner cavity wall, and the carrier releases the bacteriostatic substance.

Another method for removing the biofilm in the cavity is to inject the carrier into the cavity of the target; the nanomagnetic beads of the magnetron component are used to guide the nanomagnetic beads to drive the carrier close to the biofilm; the vibration component is controlled to generate a plurality of vibrations, and these Vibration is focused to within a limited distance of the biofilm or outside of the biofilm; these vibrations are used to generate a plurality of microbubbles that contact the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall; The heat energy destroys the heat-sensitive lipid film to release the bacteriostatic substance. Since the carrier is close to the biofilm, the bacteriostatic substance will also smoothly act on the bacteria in the biofilm.

Another method to remove the biofilm in the cavity is to inject the carrier into the cavity; use the biofilm ligand on the carrier to specifically bind to the biofilm; control the magnetron component to generate a high-frequency AC magnetic field to make the nanomagnetic inside the carrier The beads generate magnetic vibration; the magnetic vibration generates heat energy higher than the body temperature to kill the bacteria in the biofilm; the heat-sensitive lipid film of the carrier is destroyed due to the magnetic vibration and the heat energy generated by the magnetic vibration, releasing the bacteriostatic substance therein.

Another method for removing the biofilm in the cavity includes the following steps: close the vibration component to the corresponding outer surface of the cavity; control the vibration component to generate a plurality of vibrations, and focus these vibrations on the biofilm; then, use these vibrations to focus and generate A plurality of micro-bubbles in contact with the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall.

In summary, the vibration component of the present invention is used to strip most of the obvious biofilms, but it cannot remove all the bacteria in the space. Bacteria may still grow quickly after the operation, Form biofilm again. On the other hand, bacteriostatic substances cannot easily pass through the protection of the biofilm, and even if the maximum dose is increased, the bacteria in the biofilm cannot be completely removed. The system of the present invention further combines the advantages of complementing the two, using a vibrating component to peel off most of the visible biofilm, while allowing the carrier to release bacteriostatic substances, most of the peeled biofilm can flow out of the cavity and be removed by other means The bacteria or free bacteria remaining in the small amount of biofilm remaining in the cavity will be destroyed by the bacteriostatic substance. In addition to killing existing bacteria with bacteriostatic substances, environmental improvement substances can also be carried in the carrier, making it difficult for bacteria to breed in the cavity. Thus, the effect of the present invention is far superior to the existing biofilm cleaning technology. Compared with the conventional technology, the method for removing the biofilm in the cavity of the present invention can remove the biofilm deep into the inner cavity wall more clearly than the previous surgical method using physical debridement; the method of the present invention is more effective than today The removal method does not harm the patient's body tissue.

Through the detailed description of the above specific embodiments, it is hoped that the features and spirit of the present invention can be described more clearly, rather than limiting the scope of the present invention with the specific embodiments disclosed above. On the contrary, the purpose is to cover various changes and equivalent arrangements within the scope of the patent scope of the present invention.

1‧‧‧ Biofilm removal system

2‧‧‧Clear equipment

3‧‧‧Carrier

4‧‧‧Biofilm

30‧‧‧thermal grease film

32‧‧‧Bacteriostatic substance

37‧‧‧ Biofilm ligand

38‧‧‧Nano magnetic beads

Claims (10)

A biofilm removal system is applied to remove a biofilm on an inner cavity wall of a cavity. The cavity contains a liquid. The biofilm removal system includes: a carrier located in the cavity, which contains : A bacteriostatic substance for targeted inhibition of the biofilm; and a nano-magnetic bead; and a removal device, including: a magnetron component to guide and magnetically vibrate the nano-magnetic bead; and The vibrating component is used to generate a vibration to generate a plurality of micro-bubbles in the liquid, make at least a part of the biofilm detach from the inner cavity wall, and destroy the carrier to release the bacteriostatic substance. The biofilm removal system as described in item 1 of the patent application scope, wherein the vibration element further includes a plurality of vibration elements, each vibration element sequentially generates the vibration and the energy of the vibrations reaches the biofilm at the same time. The biofilm removal system as described in item 1 of the patent application scope, wherein the removal device further includes a first probe and a magnetic shielding, the magnetic shielding, the magnetron assembly and the vibration assembly are disposed on the first probe, and The magnetic shielding is located between the magnetic control component and the vibration component to reduce the electromagnetic effect of the magnetic control component on the vibration component. The biofilm removal system as described in item 1 of the patent application scope, wherein the magnetron component is further used to generate a high-frequency alternating magnetic field, and the carrier further includes a thermosensitive lipid film for coating the bacteriostatic substance and the Nano magnetic beads, and when the carrier receives the vibration generated by the vibrating element or the high frequency AC magnetic field generated by the magnetron element, the thermosensitive lipid film will be destroyed to release the bacteriostatic substance. The biofilm removal system as described in item 4 of the patent application range, wherein the carrier further comprises a biofilm ligand, and a first end of the biofilm ligand is joined to the outside of the thermosensitive lipid membrane to And a second end of the biofilm ligand is used for bonding to the biofilm. The biofilm removal system as described in item 1 of the patent application scope, wherein the bacteriostatic substance is selected from the group consisting of antibiotics, radioactive substances, antibodies, exothermic compounds, vaccines, alcohol, disinfectants and bactericides At least one of them. The biofilm removal system as described in item 1 of the patent application range, wherein the frequency of the vibration generated by the vibration component is between 0.3 MHz and 4.0 MHz. A biofilm removal system is applied to remove a biofilm on an inner cavity wall of a cavity. The cavity contains a liquid. The biofilm removal system includes: a carrier located in the cavity, which contains : An environment improving substance for improving the environment in the cavity, the environment improving substance is at least one selected from the group consisting of beneficial bacteria, acidic compounds, basic compounds, nutrients and bioremediation factors ; And a nano-magnetic bead; and a removal device, including: a magnetron component to guide and magnetically vibrate the nano-magnetic bead; and a vibrating component to generate a vibration to generate a plurality in the liquid A micro-bubble, at least a part of the biofilm from the inner cavity wall, and destroy the carrier to release the environment-improving substance. A biofilm removal system is used to remove a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes: a carrier, located in the cavity, which includes: a nano-magnetic bead; and An outer film for covering the nano magnetic beads; and a removal device, including: A vibrating component for generating a vibration to destroy the outer membrane to release the nano magnetic beads; and a magnetron component for guiding the carrier to the biofilm and generating a high frequency alternating magnetic field to magnetically vibrate the nano The rice magnetic beads generate a thermal energy to increase the temperature of the biofilm. The biofilm removal system as described in item 9 of the patent application scope, wherein a liquid is contained in the cavity, and the vibration component further focuses the vibration into the cavity to cause a vortex or a turbulent flow of the liquid. The vortex or the turbulent flow causes at least a part of the biofilm to detach from the inner cavity wall.

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