CN206494941U - A kind of Experimental Ultrasonic culture dish - Google Patents

A kind of Experimental Ultrasonic culture dish Download PDF

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Publication number
CN206494941U
CN206494941U CN201720111431.4U CN201720111431U CN206494941U CN 206494941 U CN206494941 U CN 206494941U CN 201720111431 U CN201720111431 U CN 201720111431U CN 206494941 U CN206494941 U CN 206494941U
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China
Prior art keywords
culture dish
small
ring side
connection ring
ultrasonic
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CN201720111431.4U
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Chinese (zh)
Inventor
廖晓玲
徐文峰
蒋欢
李�浩
潘吉松
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Chongqing University of Science and Technology
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Chongqing University of Science and Technology
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Abstract

The utility model provides a kind of Experimental Ultrasonic culture dish.Used Experimental Ultrasonic is made up of with culture dish small culture dish and upper culture dish, small culture dish is diameter phi standard in≤φ 35mm small dimension culture dish, connection ring side is machined with the opening of small culture dish, connection ring side is fitted in the bottom of height h >=20mm upper culture dish.The centre of the bottom of upper culture dish is machined with the intercommunicating pore for connecting small culture dish.The utility model has technical pattern form simple, is easy to use, and manufacture craft is simple, the characteristics of manufacturing cost is cheap.Solving thick ultrasonic probe needs to go deep into the problem of Tissue Culture Dish.

Description

A kind of Experimental Ultrasonic culture dish
Technical field
The utility model is related to the Tissue Culture Dish of a kind of bioengineering and medical experiment, from using being a kind of A kind of Experimental Ultrasonic culture dish tested dedicated for ultrasonic microbubble living cells.
Background technology
Ultrasonic wave has extensive purposes among our life, wherein in terms of medical application, it is most commonly used not Excessively it is used for medical diagnosis, i.e., the ultrasound medicine image technology that we frequently refer to.Acoustic contrast agent is also referred to as ultrasonic microbubble, acoustics Reinforcing agent, is the medicament of a class intravenous administration, and it mainly wraps up nothing by the macromolecular material with good biological compatibility The microbubble of malicious inert gas formation, can strengthen the backscattering of ultrasound, so that the echo-signal and blood flow that strengthen blood flow exist Doppler signal in blood vessel, can pass through pulmonary circulation, systemic organs' tissue, lesion Echoenhance be realized, so as to improve Organize the definition of development.
At this stage, ultrasonic microbubble is diffused as microvesicle and ultrasound two rapidly via the effect of initially single developer Person links together, the diagnosis and treatment for disease.This field becomes the focus that domestic and foreign scholars fall over each other research.Including: HIFU(High intensity focused ultrasound are high intensity focused ultrasound)Combining ultrasonic microbubble contrast agent is treated Tumour;Targeted ultrasound microbubble contrast agent and ultrasound molecular imaging;Ultrasonic nano microvesicle is used for gene therapy;Ultrasound microbubble contrast agent Targeted therapy;Ultrasound microbubble contrast agent carries the research of gene and drug therapy;Ultrasound microbubble contrast agent is high-strength focused super The research in sound synergy field etc..Either HIFU or targeted therapy, are different from the medication administration on conventional meaning, in order that The purpose of the accurate diagnoses and treatment of modern medicine is better achieved.
, it is necessary to by living cells culture dish, focus on Experimental Ultrasonic device and living in these substantial amounts of external living cells experiments Cell image detector, such as fluorescence inverted microscope, triplicity is together.Implementing observation using fluorescence inverted microscope In the ultrasonic microbubble experiment of living cells image, thick focus ultrasonic probe is goed deep into 15 mm-30 mm under liquid level of solution, so The ultrasonic wave of focusing is excited afterwards, the microvesicle that fixed point is transported into living cells is broken up, instantaneously collection is relevant thin for fluorescence inverted microscope Extracellular molecule signal of video signal.This process of the test gos deep into asking for 15 mm-30mm under liquid level of solution, it is necessary to solve focus ultrasonic probe Topic, and general ordinary cells culture dish can not meet this requirement.
Therefore, the utility model solves this problem, there is provided a kind of one kind tested dedicated for ultrasonic microbubble living cells The technical scheme of Experimental Ultrasonic culture dish.
The content of the invention
To solve the above problems, the technical solution of the utility model is:A kind of Experimental Ultrasonic culture dish, by small culture dish With upper culture dish composition.Small culture dish is diameter phi standard at≤φ 35mm small dimension culture dish, the opening of small culture dish Connection ring side is machined with, connection ring side is fitted in the bottom of height h >=20mm upper culture dish.The center of the bottom of upper culture dish Position is machined with the intercommunicating pore for connecting small culture dish.The actual purpose of design is to solve thick ultrasonic probe to go deep into Tissue Culture Dish Problem.
In above-mentioned technical proposal, the small culture dish can be the culture vessel with glass bottom for installing glass bottom additional, and solution must be used The experiment use requirement of glass bottom ware.Strength is machined with the connection ring side to paste, and has strongly sticked protective film.Design is favourable It is combined in facilitating cell culture, and beneficial to flexible using culture dish.
In above-mentioned technical proposal, it is in funnelform funnel ware that the upper culture dish, which can be, and the height h of funnel ware >= 30mm.Shape is different, but function phase is same, all in protection domain of the present utility model.The diameter phi of upper culture dishOnWith small culture The diameter phi of wareUnderRelation be φOn—φUnder≥20mm.Ensure diameter above, in favor of being put into ultrasonic probe.
In above-mentioned technical proposal, the small culture dish and upper culture dish or one is disposably integrally processed into by mould Body, it is not necessary to fitted with connection ring side.Processing method is different, but function phase is same, all in protection domain of the present utility model.
The utility model is can not to meet the deficiency of special Experimental Ultrasonic requirement to solve prior art, is proposed a kind of special Culture dish of the door in Experimental Ultrasonic.
The utility model compared with the existing technology, has the following advantages that:(1)This technology structure type is simple, is easy to processing With use.(2)Ingenious in design, manufacture craft is simple, and manufacturing cost is cheap.
Brief description of the drawings
Fig. 1 is the schematic perspective view on the connection ring side outside a kind of intercommunicating pore of the utility model.
Fig. 2 is the schematic perspective view on the connection ring side in a kind of intercommunicating pore of the utility model.
Fig. 3 is a kind of schematic perspective view on connectionless ring side of the utility model.
Fig. 4 is a kind of schematic perspective view of specific size of the utility model.
Fig. 5 is a kind of schematic perspective view with funnel ware of the utility model.
In figure:1. small culture dish;2. on culture dish;3. intercommunicating pore;4. connection ring side;5. glass bottom;6. funnel ware.
Embodiment
Further the utility model is illustrated with reference to the accompanying drawings and examples.
Embodiment one
Referring to figs. 1 to Fig. 4 shape and structure, a kind of Experimental Ultrasonic culture dish and application method.The small diameter phi of culture dish 1 =35mm, height 17mm;Diameter phi=60mm of upper culture dish 2, height h=20mm;3 diameter phis of intercommunicating pore=15mm.
Embodiment two
The shape and structure of reference picture 5, a kind of Experimental Ultrasonic culture dish and application method.The small diameter phi of culture dish 1= 30mm, height 15mm;Opening diameter φ=55mm of funnel ware 6, height h=35mm, in funnel-form;The diameter phi of intercommunicating pore 3= 30mm。

Claims (4)

1. a kind of Experimental Ultrasonic culture dish, by small culture dish(1)With upper culture dish(2)Composition, it is characterised in that:Small culture dish (1)Diameter phi standard≤φ 35mm small dimension culture dish, small culture dish(1)Opening at be machined with connection ring side(4), Connection ring side(4)It is fitted in height h >=20mm upper culture dish(2)Bottom;Upper culture dish(2)The centre of bottom add Work has the small culture dish of connection(1)Intercommunicating pore(3).
2. a kind of Experimental Ultrasonic culture dish according to claim 1, it is characterised in that:The small culture dish(1)Can It is to install glass bottom additional(5)Culture vessel with glass bottom;The connection ring side(4)On be machined with strength paste, strongly sticked protection it is thin Film.
3. a kind of Experimental Ultrasonic culture dish according to claim 1, it is characterised in that:The upper culture dish(2)Can It is in funnelform funnel ware to be(6), funnel ware(6)Height h >=30mm;Upper culture dish(2)Diameter phiOnWith small culture dish (1)Diameter phiUnderRelation be φOn—φUnder≥20mm。
4. a kind of Experimental Ultrasonic culture dish according to claim 1, it is characterised in that:The small culture dish(1)With it is upper Culture dish(2)Or one is disposably integrally processed into by mould, it is not necessary to use connection ring side(4)Laminating.
CN201720111431.4U 2017-02-07 2017-02-07 A kind of Experimental Ultrasonic culture dish Active CN206494941U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106635793A (en) * 2017-02-07 2017-05-10 重庆科技学院 Culture dish for ultrasonic experiment and use method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106635793A (en) * 2017-02-07 2017-05-10 重庆科技学院 Culture dish for ultrasonic experiment and use method

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