TW201940145A - Method of testing dressing composition with color change indicating function capable of determining the current state of a wound by a color change effect to reduce unnecessary replacement of wound dressings - Google Patents

Abstract

The present invention relates to a method of testing a dressing composition with a color change indicating function, in which a color change indicating film of the dressing composition is formed by using an electrospinning process and coating with an ellagic acid solution. Furthermore, the color change indicating film is irradiated with ultraviolet light, and a color change effect produced by the reaction of the ellagic acid solution and metal ions is used to determine the current state of a wound, thereby reducing unnecessary replacement of wound dressings. The method includes the steps of: taking a dressing composition; irradiating a color change indicating film of the dressing compositions with ultraviolet light to enable the color change indicating film to emit a blue-green light, wherein the color change indicating film is provided with an ellagic acid solution; and reacting the ellagic acid solution of the color change indicating film and metal ions to enable the color change indicating film to emit blue-purple light under the ultraviolet light.

Description

Test method for dressing composition with discoloration indicating function

The invention is a division of application 106145819. The invention relates to a test method for a dressing composition, in particular to a dressing composition having a discoloration indicating function.

The skin is the largest tissue and organ of the human body, and it is also the first line of defense against foreign diseases. In case of trauma, it is necessary to heal the wound quickly and restore defense.

According to recent scientific and statistical reports, the demand for the global trauma market is growing, especially in surgical trauma. The total number of people in the global trauma market has exceeded 100 million person-times each year, and it is increasing year by year. Every year, about 20 million people suffer trauma and lacerations due to accidents, and the number of burns and scalds is about 10 million people per year. In addition, ulcerative wounds caused by chronic diseases, diabetes, and aging have exceeded 3 each year. Ten million people.

Nowadays, surgery has greatly reduced the risks caused by surgery, but with it comes the need for better wound care and the prevention of postoperative scars. Wound care methods have been developed with the help of various advanced dressings to shorten wound healing time and eliminate scars at the same time.

Generally speaking, the requirements for traditional wound dressings include: (1) non-toxic and biocompatible, (2) effective isolation of pathogens, and (3) providing antibacterial and hemostatic functions.

Wound dressings are an important class of medical and cosmetic products. Among them, medical dressings are materials that cover wounds. Their main function is to control wound exudate and protect the wound from bacteria and dust particles. Although traditional gauze dressings can protect wounds, they do not promote wound healing and poor exudate management. When changing dressings, it is easy to cause discomforts such as repeated injuries and pain, scar formation, etc. due to adhesion of wounded new tissue, so it is difficult to satisfy modern people Demand for appearance and timeliness.

Therefore, the treatment mode of the wound has gradually changed from "dry wound healing" to "wetting wound healing". As for wound dressings, they have also changed from traditional dressings such as gauze and tape to wet dressings such as hydrophilic dressings. Recently there are also biological material dressings or functional dressings. As for the choice of dressings today, compared with the past, the demands for ingredients are pure natural, high absorption and low back permeability.

As far as silk protein is concerned, silk protein is a natural biopolymer protein. Its main components are sericin <fibrin> and silk fibroin <fibroin>. Sericin accounts for about 20% to 30% of the total silk protein. %, Silk fibroin content accounts for 70% to 80% of the total silk protein.

Silk fibroin contains 18 kinds of amino acids, of which glycine (gly), alanine (ala) and serine (ser) account for about 80% of the total composition. Silk fibroin itself has good mechanical properties and physical and chemical properties, such as good flexibility and tensile strength, breathable moisture permeability, slow release, etc., and different forms can be obtained through different treatments, such as fiber, solution, powder, Film and gel.

However, sericin is structurally composed of 18 different amino acids, of which 32% are serine. Moreover, sericin itself has the functions of inhibiting free radicals and anti-oxidation, can resist the skin's erosion by ultraviolet rays in sunlight, and can inhibit the activity of tyrosinase to thereby inhibit the production of melanin in the skin.

However, the wound dressing still faces problems in the condition, such as the humidity of the dressing, the degree of wound healing, and the time of replacement, such as when the wound dressing needs to be replaced or the wound dressing is wet. The wound state cannot be easily seen by the appearance of the wound dressing, and the wound state is often not known until the wound dressing is changed. For this reason, excessive wound dressings are often wasted.

Therefore, how to judge the state of the wound based on the appearance of the wound dressing without changing the wound dressing. Problems to be solved by those skilled in the art.

The main object of the present invention is to provide a test method for a dressing composition having a discoloration indicating function. The current state of the wound is judged by the discoloration of the discoloration indicator film on the dressing. To reduce unnecessary wound dressing changes.

In order to achieve the above-mentioned objects and effects, the present invention discloses a method for preparing a dressing composition with a color-changing indicator function. After the electrospinning process, the electrospin protein / polyvinyl alcohol mixed solution is used to form a non-woven fabric film, and then the ellagic acid solution is applied to form a discoloration indicating film. The discoloration indicating film is placed on a hydrophilic substrate formed by a sericin / polyvinyl alcohol mixed solution, and processed to form a dressing composition. The state of the wound was judged by the discoloration effect of the ellagic acid solution on the dressing composition.

The present invention provides an embodiment, the content of which is a method for preparing a dressing composition with a color-change indicating function, wherein the first mixed solution is a silk fibroin / polyvinyl alcohol mixed solution.

The present invention provides an embodiment, the content of which is a method for preparing a dressing composition with a color-change indicating function, wherein the second mixed solution is a sericin / polyvinyl alcohol mixed solution.

The present invention provides an embodiment, the content of which is a method for preparing a dressing composition with a discoloration indicating function. Before performing an electrospinning process, electrospinning the first mixed liquid to a circular sheet to form a non-woven film, The method further includes the step of adjusting an electrospinning parameter.

The present invention provides an embodiment, the content of which is a method for preparing a dressing composition with a color-change indication function, wherein the electrospinning parameter further includes a voltage value, a working distance, and a flow speed.

The present invention provides an embodiment, the content of which is a method for preparing a dressing composition with a discoloration indicating function, wherein the ellagic acid solution must be subjected to an alcohol treatment in advance.

In addition, the present invention discloses a dressing composition having a discoloration indicating function, comprising: a hydrophilic substrate; and a discoloration indicating film disposed on the hydrophilic substrate, and the discoloration indicating film comprises a silk fibroin / poly A mixed solution of vinyl alcohol and an ellagic acid solution, and the ellagic acid solution forms a color-change indicator pattern on the color-change indicator film.

The present invention provides an embodiment, the content of which is a dressing composition with a color-change indicating function, wherein the hydrophilic matrix comprises a sericin / polyvinyl alcohol mixed solution.

In addition, the present invention discloses a method for inspecting a dressing composition having a discoloration indicating function, the steps include: taking a dressing composition; and irradiating a discoloration indicating film of the dressing composition with an ultraviolet light, the discoloration indicating film emits A blue-green light, the discoloration indicating film comprises a ellagic acid solution; and when the ellagic acid solution of the discoloration indicating film reacts with a metal ion, the discoloration indicating film emits a blue-violet light under the ultraviolet light.

The present invention provides an embodiment, the content of which is a method for inspecting a dressing composition having a discoloration indicating function, wherein the metal ion is derived from an interstitial fluid.

The present invention provides an embodiment, the content of which is a method for inspecting a dressing composition having a discoloration indicating function, wherein the wavelength of the ultraviolet light is 365 nm.

In order to make the reviewing committee members have a better understanding and understanding of the features of the present invention and the effects achieved, I would like to provide examples and cooperative descriptions, as described below:

In view of the fact that the wound state cannot be easily seen by the appearance of the wound dressing, the wound state is often known only after the wound dressing is changed, and the effect of excessive wound dressing is wasted. Based on this, the present invention proposes a dressing composition with a discoloration indicator Test methods to solve the problems caused by conventional technology.

In the following, the characteristics included in the inspection method of the dressing composition with a discoloration indicating function of the present invention, the matched structure and the method thereof will be further explained:

Please refer to FIG. 1, which is a flowchart of the preparation method of the present invention. As shown in the figure, a method for preparing a dressing with a discoloration indicating function according to the present invention includes the following steps:

S1: preparing a first mixed solution and a second mixed solution. The first mixed solution includes silk fibroin protein (SFP) and polyvinyl alcohol, and the second mixed solution includes silk sericin protein (SSP) and Polyvinyl alcohol

S3: Perform an electrospinning process, and electrospin the first mixed solution to form a non-woven film on the circular sheet;

S5: apply the ellagic acid solution on the non-woven film to form a discoloration indicating pattern, and form a discoloration indicating film;

S7: placing the color-changing indicator film inside the round dish and covering the round mold cover, adding the second mixed liquid to the round mold cover, and freeze-drying to form a hydrophilic matrix; and

S9: After removing the round plate and the round mold cover, a dressing composition is obtained.

As shown in step S1, weigh 50mg of silk sericin protein (SSP) into a clean sample bottle, and draw 2ml of polyvinyl alcohol (PVA) (25 mg / ml) and add The sample bottle is mixed with the sericin and rapidly stirred with a magnetic stirrer to form a second mixed solution, and the second mixed solution is a sericin / polyvinyl alcohol mixed solution.

Furthermore, 60 mg of silk fibroin protein 1 (SFP) and 40 mg of the polyvinyl alcohol (PVA) were weighed into a clean sample bottle, and 1 ml of formic acid was added to the sample bottle and the The silk fibroin 1 and the polyvinyl alcohol are mixed and quickly stirred with a magnetic stirrer to form a first mixed liquid, and the first mixed liquid is a silk fibroin / polyvinyl alcohol mixed liquid.

As shown in step S3, before performing an electrospinning process, electrospinning the first mixed solution to form a non-woven film on a circular sheet, first perform a step of adjusting an electrospinning parameter, wherein the electrospinning parameter is further It includes a voltage value, a working distance, and a flow speed.

Following the above, the dehumidifier was turned on to control the humidity of the experimental environment between 40 and 45%, and the first mixed solution was sucked with a syringe and placed in an electrospinning device. Adjust the electrospinning parameters (the voltage value: 17kV, the working distance: 7.5cm, the flow speed: 5µl / min), and then form the first mixed liquid electrospun on the circular sheet to form a film. Non-woven film.

Continuing as shown in step S5, before the steps of applying a ellagic acid solution on the non-woven fabric film to form a discoloration indicator pattern 322 and forming a discoloration indicator film 32, the ellagic acid solution must be subjected to an alcohol treatment in advance. .

Following the above, an ellagic acid (EA) was heated and dissolved in an alcohol (300 µg / ml) to prepare the ellagic acid solution.

After the non-woven fabric film is fixed, the ellagic acid solution is coated on one surface of the non-woven fabric film to form the discoloration indicator pattern 322, and the discoloration indicator pattern 322 is a cross shape. Then put it into a vacuum pump to dry the ellagic acid solution of the discoloration indicator pattern 322 and repeat the process 4 times to obtain the discoloration indicator film 32, wherein the absorption wavelength of the discoloration indicator film 32 is 365 nm.

Furthermore, as shown in step S7, the color-changing indicating film 32 (the color-changing indicating icon 322 faces downward) is placed inside the circular dish and covered with a circular mold cover.

And apply a layer of the second mixed liquid on the color-changing indicating film 32, and then freeze it at -80 ° C for 1 minute. After taking out, repeat this step once. After forming an ice layer on the color-changing indicating film 32, pour the The second mixed liquid was placed in the circular mold cover and frozen at -80 ° C for 24 hours, and then subjected to freeze-drying treatment for 24 hours to obtain a hydrophilic matrix 30.

Finally, as shown in step S9, after removing the round plate and the round mold cover, a dressing composition 3 is obtained.

Please also match FIG. 2, which is an absorption light spectrum chart of the color-changing indicating film of the present invention. It can be seen from the figure that the peak of the absorption wave of the discoloration indicator pattern (elfin acid solution + silk fibroin) added with the ellagic acid solution is 365 nm.

Please also match Figs. 3A-3B, which are schematic structural diagrams of the color-changing indicating film of the present invention. The indication principle of the color-changing indicator film is that fluorescence usually occurs in compounds of low energy transition type π → π *, because the Mohr absorption coefficient of π → π * transition is usually 100 to 100 times larger than the process of n → π *. 1000 times. The silk fibroin 1 in the discoloration indicating film has a fibroin β sheet structure, so that the part that is not coated with the ellagic acid solution absorbs electrons and then transitions from π to π * and releases After the energy, blue-violet light is emitted (as shown in Figure 3A).

In the cross-shaped coating of the ellagic acid solution, the ellagic acid 2 is bound between the silk fibroin β sheet structure of the silk fibroin 1, and the -OH of the ellagic acid 2 is helpful A type of chromophore, the chromophore itself will not absorb radiation in the ultraviolet range, but will shift the peak lines with chromophores to longer wavelengths and increase the intensity of the peak lines. Therefore, the change in structure and the influence of the chromophore shift the absorption peak wavelength to the long wave direction (red shift) and change the light emission wavelength to the long wavelength. When the energy absorbed by π → π * of the silk fibroin 1 is released, The electron drops to a smaller π orbital range of the ellagic acid 2 and emits smaller energy to emit light, showing a longer blue-green fluorescent light (as shown in FIG. 3B).

Continuing with Figure 4, it is a schematic diagram of a dressing composition with a color-changing indicator function according to the present invention. As shown in the figure, a dressing composition 3 having a discoloration indicating function includes: the hydrophilic substrate 30; and the discoloration indicating film 32 disposed on the hydrophilic substrate 30, and the discoloration indicating film 32 includes the The silk fibroin / polyvinyl alcohol mixed solution and the ellagic acid solution, and the ellagic acid solution formed the discoloration indicating pattern 322 on the discoloration indicating film 32. The hydrophilic matrix 30 comprises the sericin / polyvinyl alcohol mixed solution.

Please refer to Figure 5, which is a flowchart of the inspection method of the present invention. As shown in the figure, a method for inspecting a dressing composition with a discoloration indicating function according to the present invention includes the following steps:

S2: take the dressing composition;

S4: When the discoloration indicating film of the dressing composition is irradiated with ultraviolet light, the discoloration indicating film emits blue-green light, and the discoloration indicating film includes a ellagic acid solution; and

S6: After the ellagic acid solution of the discoloration indicating film reacts with metal ions, the discoloration indicating film emits blue-violet light under ultraviolet light.

As shown in step S2, the dressing composition is taken and placed on a wound.

Continuing step S4, when one of the dressing composition 3's color-changing indicating film 32 is irradiated with an ultraviolet light, the color-changing indicating film 32 emits a blue-green light, and the color-changing indicating film 32 includes the ellagic acid solution. FIG. 6A is a light emission spectrum chart of the color-changing indicating film of the present invention. It can be seen from the figure that the cross-shaped portion (elfinic acid solution + silk fibroin) added to the ellagic acid solution is the blue-green light, and the portion (filin protein) that is not coated with the ellagic acid solution is Blue-purple light.

Then, as shown in step S6, after the ellagic acid solution of the discoloration indicating film 32 reacts with a metal ion, the discoloration indicating film 32 emits the blue-violet light under the ultraviolet light, wherein the metal ion comes from a Tissue fluid and the wavelength of the ultraviolet light is 365 nm.

Please also match Figures 6B-6C, which are the light spectrum spectra of the color-changing indicator film of the present invention. It can be seen from the figure that the interstitial fluid can be a deionized water (DI) or a simulated body fluid (SBF), the ellagic acid solution in the cross-shaped part and the deionized water or the simulated body fluid After the metal ions react, the wavelength of light is shifted, and then the blue-violet light that is the same as the portion where the ellagic acid solution is not applied is emitted.

Please continue to match Fig. 6D, which is a light emission spectrum diagram of the color-changing indicating film of the present invention. It can be seen from the figure that when the ellagic acid solution in the cross-shaped portion reacts with the metal ion on a matrix metalloproteinase 2 (MMP2) secreted by the wound, it will also shift the wavelength of light, and then emit The blue-violet light is the same as the portion where the ellagic acid solution is not applied.

Please refer to FIG. 7 for the continuation, which is a use state diagram of the dressing composition with a discoloration indicating function of the present invention. As can be seen from the figure, after the dressing composition 3 has passed for a period of time (24 hours), the ellagic acid solution in the cross-shaped portion reacts with the deionized water or the metal ion of the human body simulated body fluid, and gradually changes its Shade.

Therefore, the present invention is truly novel, progressive, and available for industrial use. It should comply with the patent application requirements of China's patent law. No doubt, the invention patent application was filed in accordance with the law.

However, the above are only preferred embodiments of the present invention, and are not intended to limit the scope of implementation of the present invention. For example, all changes and modifications of the shapes, structures, features, and spirits in accordance with the scope of the patent application for the present invention are made. Shall be included in the scope of patent application of the present invention.

1‧‧‧ silk fibroin

2‧‧‧ Ellagic acid

3‧‧‧ dressing composition

30‧‧‧ hydrophilic matrix

32‧‧‧Discoloration indicating film

322‧‧‧Color Change Indicator

S1-S9‧‧‧step flow

Figure 1: It is a flowchart of the preparation method of the present invention;

Figure 2: It is the absorption light spectrum chart of the color-changing indicating film of the present invention;

3A-3B: a schematic diagram of the structure of the color-changing indicator film of the present invention;

FIG. 4 is a schematic diagram of a dressing composition having a discoloration indicating function according to the present invention;

FIG. 5 is a flowchart of the inspection method of the present invention;

Figures 6A-6D: a light emission spectrum of the color-changing indicator film of the present invention; and

FIG. 7 is a diagram showing a state of use of a dressing composition having a discoloration indicating function according to the present invention.

Claims (3)

A test method for a dressing composition with a discoloration indicating function, the steps include: Take a dressing composition; When one of the discoloration indicating films of the dressing composition is irradiated with an ultraviolet light, the discoloration indicating films emit a blue-green light, and the discoloration indicating films include a ellagic acid solution; and When the ellagic acid solution of the discoloration indicating film reacts with a metal ion, the discoloration indicating film emits a blue-violet light under the ultraviolet light. The test method for a dressing composition with a discoloration indicating function as described in item 1 of the scope of the patent application, wherein the metal ion is derived from an interstitial fluid. The inspection method of the dressing composition with a discoloration indicating function as described in item 1 of the scope of the patent application, wherein the wavelength of the ultraviolet light is 365 nm.