TW201907943A - A use of an extract of Bombax malabaricum DC - Google Patents

Abstract

A use of an extract of Bombax malabaricum DC. in the manufacture of a medicament for protection of chemical-induced hepatotoxicity is disclosed. The extract of Bombax malabaricum DC. is extracted by water at 115-125 DEG C, 0.35-0.5 Kg/cm2G for 1.5-3 hours.

Description

 Use of kapok water extract  

The invention relates to the use of an aqueous extract of kapok, in particular to the use of an aqueous extract of kapok in the preparation of a medicament for preventing and treating chemical liver injury.

The liver is a metabolically active organ in the human body and plays a role in removing toxins. When the human body is exposed to chemical toxins, chemical toxins are transported to the liver for metabolism. However, the accumulation of a large amount of chemical toxins causes chemical liver damage, which causes acute hepatitis or even fulminant. Hepatitis) and other symptoms.

Severe hepatitis refers to a sudden and severe loss of liver function. It is currently considered to be the best treatment for liver transplantation. However, the donated liver is not easy to obtain, and common contraindications after surgery are common. Therefore, chemical liver injury is still prevented. Avoid the occurrence of violent hepatitis as the first means.

In order to solve the above problems, the present invention provides a use of an aqueous extract of kapok, which is applied to a medicament for preparing a chemical liver injury by extracting an active ingredient extracted from kapok.

The use of the kapok aqueous extract of the invention is applied to the preparation of a medicament for preventing and treating chemical liver injury, wherein the kapok aqueous extract is at a temperature of 115 to 125 ° C, 0.35 to 0.5 Kg/cm ² G. The extraction is carried out under pressure for 1.5 to 3 hours. Preferably, the kapok aqueous extract can be administered orally to a desired individual, and the kapok aqueous extract is administered to the desired individual daily. It is 2~6ml/kg body weight; thus, by extracting the active ingredients from kapok, it can reduce the increase of liver function index caused by carbon tetrachloride and slow down the necrosis of liver cells caused by carbon tetrachloride. Therefore, it can be applied to the preparation of a medicament for preventing and treating chemical liver damage, which is an effect of the present invention.

Figure 1a: Results of the effect of the aqueous extract of Kapok according to the invention on liver function index GOT/AST in test (A).

Figure 1b: Test (A), the effect of the extract of the Kapok extract of the present invention on the liver function index GPT/ALT.

2a~2d: In the test (B), the effect of the aqueous extract of the kapok of the present invention on hepatocyte necrosis was administered.

The above and other objects, features and advantages of the present invention will become more <RTIgt;

"Kapok (Bombax malabaricum DC.)" Of the present invention, means Bombacaceae (Bombacaceae) bombax (malabaricum) of the plant, its flowers (ie, Kapok) detoxification, often used in soup.

The kapok water extract of the invention can reduce the liver function index caused by carbon tetrachloride and slow down the hepatocyte necrosis caused by carbon tetrachloride, so it can be applied to prepare chemical liver The damaged drug, the kapok aqueous extract is combined with a pharmaceutically acceptable carrier or excipient to form a pharmaceutical composition, wherein the kapok aqueous extract can be prepared into any convenient form, such as an ingot. The agent, the capsule, the powder, the granule or the liquid agent, or the like, or the kapok water extract is combined with other foods or beverages, and is suitable for eating for the organism to be taken orally.

Further, the kapok aqueous extract of the present invention can be administered to a desired individual, for example, by administering the desired individual at a dose of 2 to 6 ml/kg of body weight to impart activity to the kapok aqueous extract. The component can act in the desired individual, and can reduce the increase of liver function index caused by carbon tetrachloride and slow down the hepatocyte necrosis caused by carbon tetrachloride.

Wherein, the kapok aqueous extract is preferably obtained by a method comprising the steps of: providing a kapok sample; extracting the kapok sample with water as an extraction solvent to obtain a kapok crude extract; And concentrating the kapok crude extract to obtain the kapok aqueous extract.

In detail, the kapok sample may be preliminarily dried at a temperature of 110 to 120 ° C (100 g of the kapok sample is dried to 20 g) to obtain a kapok dried product; in addition, the kapok sample is also It can be preliminarily broken into powder particles (having a particle size of about 1 to 2 mm) to increase the contact surface area of the kapok sample with the extraction solvent, thereby improving the extraction efficiency of the subsequent extraction.

For example, each 4.8 kg of kapok sample can be mixed with the extraction solvent to a total volume of 80 liters, and extracted at a temperature of 115 to 125 ° C and a pressure of 0.35 to 0.5 Kg/cm ² G. ~3 hours, the above extraction may also be repeated several times, so that the active ingredient rich in the kapok sample can be completely dissolved in the extraction solvent, which is widely used by those having ordinary knowledge in the technical field of the invention. Repeat them.

The aforementioned kapok crude extract can be concentrated under reduced pressure and freeze-dried to obtain the kapok aqueous extract. By this procedure, the active constituents of the kapok aqueous extract can be more concentrated, so that only The best effect can be achieved by using a small amount of this kapok water extract.

In order to confirm that the Kapok water extract system of the present invention can reduce the liver function index caused by carbon tetrachloride and slow down the hepatocyte necrosis caused by carbon tetrachloride, the 4.8 kg of kapok will be used. The crude kapok extract obtained by the sample extraction was concentrated under reduced pressure and freeze-dried to obtain a total of 800 g of the kapok aqueous extract powder, and after 5 g of the kapok aqueous extract powder was dissolved in 20 ml of water, the following test was carried out.

(A) Effect of Kapok Water Extract on Liver Function Index

In this test, male Wistar rats (200-250 g) were used for the test, and the rats of each group were continuously orally administered with the extract of Kapok as shown in Table 1 for 4 days (once a day), on the fourth day. The rats were intraperitoneally injected with carbon tetrachloride (dissolved in olive oil at a concentration of 20%). At 24 hours, rats in each group were sacrificed, and blood was collected by cardiac blood sampling to analyze the liver function index of each group. The results are as shown in 1a. , Figure 1b.

Please refer to the figures 1a and 1b. The water extract of Kapok with a concentration of 2~6mL/kg can effectively reduce the increase of liver function index caused by carbon tetrachloride (Groups A2 and A3).

(B) Effect of Kapok aqueous extract on necrotic hepatocytes

In this experiment, male Wistar rats were also used for the test. The rats were continuously orally administered with the extract of Kapok as shown in Table 2 for 4 days (once a day), and intraperitoneal injection of tetrachlorochloride on the 4th day. Carbon (dissolved in olive oil, concentration 20%), sacrificed rats in 24 hours, collected liver, sliced and hematoxylin and eosin stain, H&E stain, the results are as follows Figure 2a~2d shows.

Please refer to Figure 2b for the hepatocyte necrosis near the central vein (CV) of the liver of the B1 group of rats treated with carbon tetrachloride, and the overall tissue structure is disordered; The phenomenon of hepatocyte necrosis near the central vein of rats in the B2 and B3 groups of 2-6 mL/kg of Kapok water extract was slowed, indicating that the Kapok water extract of the present invention can effectively alleviate carbon tetrachloride. The phenomenon of hepatocyte necrosis caused.

In summary, the use of the kapok aqueous extract of the present invention can reduce the increase in liver function index caused by carbon tetrachloride and slow down the liver caused by carbon tetrachloride by extracting the active ingredient from kapok. The phenomenon of cell necrosis can be applied to the preparation of a drug for preventing and treating chemical liver damage, which is an effect of the present invention.

While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.

Claims (3)

The invention relates to a method for preparing a water extract of kapok, which is used for preparing a medicament for preventing and treating chemical liver injury, wherein the water extract of the kapok is water as an extraction solvent at a temperature of 115 to 125 ° C, 0.35 to 0.5 kg/ A kapok sample was extracted under a pressure of cm ² G for 1.5 to 3 hours.  The use of the kapok aqueous extract according to claim 1, wherein the kapok aqueous extract is administered orally to a desired individual.    The use of the kapok water extract according to claim 2, wherein the kapok water extract is administered to the desired individual at a dose of 2 to 6 ml/kg body weight per day.