TW201900861A - Wet detergent wipe - Google Patents

Abstract

The invention relates to a moist detergent wipe composed of a non-woven support impregnated with a detergent solution, the detergent solution comprising a protease, a lipase and an amylase, characterized in that the non-woven support comprises polyester fibers.

Description

Wet detergent wipes

The present invention relates to a wet detergent wipe impregnated with an enzyme detergent solution. These wipes are particularly suitable for cleaning and disinfection in hospitals and medical fields, especially hard surfaces and medical equipment and instruments, such as endoscopes.

Wet detergent wipes are currently widely used in the medical and hospital fields to simply and quickly clean hard surfaces such as work surfaces and medical equipment and instruments. Generally, these wipes are impregnated with a detergent solution containing one or more surfactants and possibly antimicrobial agents such as quaternary ammonium salts. Such wipes are sold by the applicant under the trade name WIP'ANIOS®, for example.

Patent application WO 00/37602 A1 discloses an enzyme detergent composition comprising a non-ionic surfactant or a mixture of non-ionic and anionic surfactants, a protease, an amylase and possibly a lipase, and a stable enzyme activity for cleaning System for blood and other organic stains. Such enzyme activity stabilizers listed in the patent application are calcium salts, linear or branched alcohols (such as ethanol or isopropanol), alkanolamines (such as triethanolamine), acids (especially organic acids), and petroleum Distillate. Enzymatic detergent compositions can be incorporated into individually packaged wipes. However, the patent application does not disclose any tangible examples of wipes impregnated with an enzyme detergent composition. It relates only to the patent US 4998984, which recommends the use of biodegradable wipes.

Glycols and other polyols, especially glycerol, are also known to stabilize enzyme detergent compositions. However, these have the disadvantage of producing greasy marks on the treated surface.

During its research, the applicant has observed that the choice of wipe material significantly affects the stability of the detergent solution, especially the enzyme activity. Therefore, as a result of extensive research, the applicant has developed a wet detergent wipe impregnated with a detergent solution that includes at least one protease, at least one amylase, and at least one lipase and remains stable over time , Which retains the enzyme activity of various enzymes, and does not leave greasy marks on the treated surface.

Therefore, the object of the present invention is a wet detergent wipe composed of a non-woven carrier impregnated with a detergent solution. The detergent solution includes a protease, a lipase, and an amylase. Ester fiber

Contrary to all expectations, the choice of a non-textile carrier comprising polyester fibers, especially polyethylene terephthalate, on the one hand provides good impregnation of the detergent solution and on the other hand maintains good enzyme activity over time. In a particularly advantageous embodiment, the wiper is composed of polyester fibers, preferably polyethylene terephthalate fibers. Preserving enzyme activity due to the presence of polyester fibers means that those skilled in the art can dispense with the addition of glycols or other polyols, especially glycerol, for this purpose, thereby avoiding greasy marks on the treated surface. In one embodiment, the detergent solution is therefore free of glycols and / or glycerol.

The wipes may be non-woven or woven. Preferably, a non-woven wipe will be used. Therefore, in a particularly preferred embodiment, the wipe is a non-woven polyethylene terephthalate wipe, that is, composed of polyethylene terephthalate fibers. In fact, this type of wiper not only impregnates the detergent solution uniformly and stably, but also maintains the enzyme activity of the detergent solution over time.

The detergent solution impregnation rate of the wipe is advantageously 100% to 500% by weight relative to the dry weight of the wipe, preferably 200% to 400% by weight and more preferably approximately 300% by weight.

The detergent solution impregnated with the wipe includes at least one protease, at least one lipase, and at least one amylase. Advantageously, the protease content is from 0.001% to 0.5% by weight relative to the total weight of the composition, preferably from 0.01% to 0.1% by weight relative to the total weight of the composition, and more preferably relative to the total weight of the composition. 0.03% to 0.07% by weight of the total weight, and even more preferably approximately 0.05% by weight relative to the total weight of the composition. The lipase content is advantageously from 0.001% to 0.5% by weight relative to the total weight of the composition, preferably from 0.01% to 0.1% by weight relative to the total weight of the composition, and more preferably relative to the total weight of the composition 0.3% to 0.07% by weight, and even more preferably approximately 0.05% by weight relative to the total weight of the composition. The amylase content is advantageously from 0.001% to 0.5% by weight relative to the total weight of the composition, preferably from 0.01% to 0.1% by weight relative to the total weight of the composition, and more preferably relative to the total weight of the composition 0.03% to 0.07% by weight, and even more preferably approximately 0.05% by weight relative to the total weight of the composition.

Considering that the main components of biological tissues that form significant stains in hospitals and medical fields are proteins, lipids (triglycerides) and carbohydrate polymers (glycogen), the trienzyme mixed solution of the concentrated aqueous detergent composition of the present invention The three objectives will be hydrolyzed simultaneously and thus promote decontamination. In addition to these three types of enzymes, detergent solutions can also include mannanase.

The detergent solution may also include one or more surfactants. These surfactants are preferably selected from non-ionic surfactants, amphoteric surfactants and mixtures thereof. Non-limiting examples of non-ionic surfactants include alkyl polyglucosides, alkyl polypentanosides, amino oxides, polyglycosides, glucosamine, polyalkoxylated fatty alcohols, and especially polyethoxylated And ethoxylated fatty acid esters. Non-limiting examples of amphoteric surfactants include cocamidopropyl betaine. Preferably, one or more surfactants are selected from non-ionic surfactants, more preferably from fatty polyalkoxylated alcohols, especially polyethoxylated; and amine oxides, especially alkylamino oxides. Substances such as dodecyldimethylamine oxide.

One or more surfactants are advantageously included in the washing in an amount of 0.001% to 2% by weight, preferably 0.001% to 1% by weight and even more preferably 0.025% to 0.5% by weight relative to the total weight of the composition剂 溶液 中。 Solution.

Detergent solutions may also contain chelating agents, especially biodegradable chelating agents. The biodegradable chelating agent is preferably selected from methyl glycine diacetate (MGDA) and imino disuccinate (IDS), especially tetrasodium imino disuccinate. Even better, the biodegradable chelator is methyl glycine diacetate (MGDA). Without wishing to be bound by any particular theory, the present inventor believes that the chelating agent implemented in the present invention is not only used to soften water, but also to be particularly effective for complexing divalent and trivalent metal ions, especially Ca ²⁺ , Fe ³⁺ , Fe ²⁺ and Cu ²⁺ , which are contained in protein residues commonly found on surgical and / or medical instruments. Obviously these chelating agents chelate these metal ions, which destabilizes the tertiary structure of the protein and makes it easier to hydrolyze.

The chelating agent content of the composition used according to the present invention is advantageously 0.001% to 2% by weight relative to the total weight of the composition, preferably 0.01% to 1% by weight relative to the total weight of the composition, more It is preferably 0.1% to 0.5% by weight relative to the total weight of the composition, and even more preferably approximately 0.27% by weight relative to the total weight of the composition.

The concentrated aqueous detergent composition is advantageously free of any bio-degradable chelating agents, especially ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), phosphates and polyphosphates. Advantageously, the concentrated aqueous detergent composition is also free of chelating agents of any reproductive toxicity classification, such as nitrogen triacetic acid (NTA) and its sodium salts.

In one embodiment, the concentrated aqueous detergent composition is also free of alkali metal and alkaline earth metal hydroxides.

Preferably, the detergent solution is an aqueous solution. Its water content is advantageously from 90.0% to 99.997% by weight relative to the total weight of the composition, preferably from 95.0% to 99.9% by weight relative to the total weight of the composition, and more preferably relative to the total weight of the composition 97.0 to 99.5% by weight and even more preferably 98.0 to 99.0% by weight relative to the total weight of the composition.

The detergent solution may also include one or more disinfectants. In this case, the wipes are no longer just detergents, but also disinfectants. Examples of the antimicrobial agent used in the present invention include, but are not limited to, a quaternary ammonium salt, an amine carrying at least one C8 to C20 alkyl group, chlorhexidine digluconate, polyhexamethyleneguanide (PHMB), or a salt thereof. Preferably, the one or more antimicrobial agents are selected from the group consisting of didecyldimethylammonium salt, didecylmethyl polyoxyethylammonium salt, benzyl ammonium salt, phenethylammonium salt, methylphenethylammonium Salt, cetalkonium salt, cetylpyridine salt, cetyltrimethylammonium salt, laurylamine, N- (3-aminopropyl) -N-dodecylpropane- 1,3-diamine and N-alkyl (C12-C14) -1,3-diaminopropane, more preferably selected from the group consisting of didecyldimethylammonium chloride, didecyldimethylammonium carbonate, Didecyl methyl polyoxyethylammonium propionate, benzyl ammonium chloride, benzethonium chloride, methyl benzethonium chloride, sitamium chloride, cetylpyridine chloride, cetyl chloride Alkyltrimethylammonium, laurylamine, N- (3-aminopropyl) -N-dodecylpropane-1,3-diamine and N-alkyl (C12-C14) -1,3- Diaminopropane, and even better selected from the group consisting of didecyldimethylammonium chloride, didecyldimethylammonium carbonate, didecylmethylpolyoxyethylammonium propionate, laurylamine, N- (3 -Aminopropyl) -N-dodecylpropane-1,3-diamine and N-alkyl (C12-C14) -1,3-diaminopropane. In a specific embodiment, the one or more antimicrobial agents are selected from the group consisting of didecyldimethylammonium chloride, didecyldimethylammonium carbonate, N- (3-aminopropyl) -N-dodecyl Alkylpropane-1,3-diamine and N-alkyl (C12-C14) -1,3-diaminopropane.

Depending on the disinfectant or disinfectants implemented, those skilled in the art can easily adjust the amount of disinfectant. In the case of a quaternary ammonium salt, its amount in the detergent solution is advantageously from 0.01% to 2% by weight relative to the total weight of the composition, preferably 0.1% by weight relative to the total weight of the composition To 1% by weight, more preferably 0.2% to 0.5% by weight relative to the total weight of the composition.

Detergent solutions may also include preservatives. The preservative can be selected from commonly used preservatives, such as 5-chloro-5-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin 3-one, p-hydroxybenzoic acid and its formazan Esters, 2-pyridinethiol-1-sodium oxide, 5-bromo-5-nitro-1,3-dioxane and salicylic acid, benzyl alcohol and sodium benzoate. These preservatives avoid microbial and fungal contamination by providing antibacterial and antifungal effects.

The detergent solution may also include other additives, such as stabilizers, such as antioxidants and / or anti-UV agents and / or dispersants and / or defoamers. The dispersant may be, for example, a homopolymer, copolymer, or terpolymer acid based on acrylic acid or an alkali metal salt (polyacrylate) thereof.

The wipes according to the invention are particularly useful for cleaning and / or disinfecting objects with hard surfaces, such as medical instruments and devices and work surfaces. Medical instruments and devices within the scope of the present invention include, in particular, endoscopes without internal passages, such as nasal fiberscopes and transesophageal probes, hemodialysis generators, ultrasonic probes, and electronic thermometers.

Therefore, a second object of the present invention is the use of the aforementioned wipes for cleaning and / or disinfecting objects having a hard surface, such as medical instruments and devices, and work surfaces.

The invention also relates to a method for cleaning and / or disinfecting objects with hard surfaces, such as medical instruments and devices and work surfaces, comprising the following steps: a) supplying wipes as defined above; b) using the The wipe wipes the hard surface of the object; and c) it is possible to rinse the hard surface with water, preferably with demineralized water.

In summary, the present invention provides an enzymatic detergent and / or disinfectant wipe which allows the elimination of the use of glycol or polyol type stabilizers, especially glycerol. By using a non-woven carrier comprising polyester fibers, especially polyethylene terephthalate, these stabilizers are no longer necessary to maintain the enzymatic activity and therefore the cleaning ability of the detergent solution with respect to organic stains.

The invention will now be described with reference to non-limiting examples of the invention.

Examples Examples 1 : Preparation of enzyme detergent solution

Detergent and disinfectant solutions S1 to S6, the formulations (% by weight relative to the total weight of the solution) are provided in Table 1, and were prepared by mixing the ingredients in the following order.

S1 to S5: demineralized water, Trilon M, Genamin LAP 100, Mergital D8, Carboquat HE, Lutropur MSA, enzyme.

S6: Demineralized water, Bardac 22/40, Genamin LAP 100, Ammonyx LO, Bar, Lutropur MSA, enzyme.

In order to prepare the sample, the ingredients were added sequentially and slowly stirred, and the contact time of stirring was maintained for 30 minutes after the final ingredients were added. Table 1 Example 2 : Stability of enzyme detergent solution

In order to study the stability of the detergent solution, only the solutions S1 and S2 (Example 1) and the wipes impregnated with these solutions (one detergent solution per batch of wipes) were placed at 30 ° C for 8 weeks and monitored during this period Enzyme stability. Protease stability is performed by electrophoresis, while the stability of other enzymes is performed by thin layer chromatography.

Electrophoresis on a polyacrylamide gel allows detection of peptides or proteins. After the protein was negatively charged by the SDS solution, it was deposited on a polyacrylamide gel placed in an electrolytic environment, and then subjected to a potential difference (DDP). The molecule then undergoes 2 antagonistic effects:-the larger the molecular weight of the protein or peptide, the more negatively charged the molecule is, and therefore the larger the molecule is more sensitive to the applied DDP;-the larger the molecule, the more difficult it will migrate within the gel matrix.

After migration, proteins and peptides are exposed after being decolorized by exposure to a coloring solution.

After contacting the albumin solution with the enzyme solution and completing the electrophoresis, the presence of a small module confirms protease activity.

Thin layer chromatography (TLC) is based on ion adsorption, sharing, and / or exchange phenomena.

TLC consists of two phases:-the mobile phase (eluent) is a solvent or solvent mixture that travels along the stationary phase;-the stationary phase consists of silicone gel that is evenly spread into a thin layer on a semi-rigid sheet.

The principle is as follows: molecules are separated by differential migration. Each molecule undergoes two antagonistic effects: a stimulating effect applied by the mobile phase and a retention effect applied by the stationary phase; the components of the mixture move and separate at different speeds.

After migration, the compound is revealed by a color reaction according to the active ingredient. It was identified as possible by means of an indicator stored under the same conditions as the unknown active ingredient.

Each active ingredient is characterized by the Rf ratio of the migration distance of its point to the migration distance in front of the solvent.

After contacting the glycogen (amylase activity) or triglyceride (lipase activity) solution with the enzyme solution and completing the thin-layer chromatography, the presence of molecules derived from the hydrolysis of glycogen and triglycerides respectively confirmed the amylase and fat Enzyme activity.

The following commercially available wipes were selected for research:-100% rayon;-100% PET (polyethylene terephthalate);-rayon / cellulose / PES acrylic adhesive;-70% rayon / 30 % Polyester;-100% polypropylene.

Polypropylene wipes cannot be impregnated. The remaining four types of wipes were impregnated with all solutions.

The enzyme stability of each formulation is regularly monitored:-on an immersion solution at 30 ° C;-on a desorption solution of a wipe after immersion at 30 ° C.

The results are listed in Tables 2 (Solution S1) and 3 (Solution S2) below. Table 2 + = Good stability;-/ + = partial stability;-= insufficient stability Table 3 + = Good stability;-/ + = partial stability;-= insufficient stability

After the solution soaked in a wipe containing human silk was desorbed, the presence of a substance extracted from the wipe was observed in the desorbed solution. When enzyme activity is highlighted, these substances cause interference and the results cannot be explained.

Regarding the other four types of wipes, after dipping on PET or rayon / cellulose / PES wipes, the stability of amylase was observed to be good regardless of the nature of the protease. Lipases are unstable in solution, but impregnation on PET or rayon / cellulose / PES wipes allows for improved behavior. Finally, after dipping on PET wipes, mannanase is stable regardless of the protease. Suspected degradation of human silk / cellulose / PES wipes and human silk / polyester wipes. Use 100% PET wipes for best results. Example 3 : Resolution of wipes

The analytical capabilities of the immersion solutions of PET (100%), rayon / cellulose / PES (acrylic adhesive) and polyester / rayon (70/30) wipes were measured as follows.

Each of the solutions S1 and S5 was immersed in 300% of various wipes. The wipes were then pressed with the aid of a 50 ml syringe (one wipe per syringe) to recover the impregnating fluid and measure the proteolytic activity of the solution, where the solution used for impregnation was used as a reference.

Regardless of the formulation, the best resolution is obtained on PET wipes. Example 4 : Enzyme stability and biocidal activity of detergent and disinfectant solution S6

100% PET wipes were impregnated with 300% by weight detergent and disinfectant solution S6. Enzyme stability studies were performed on a desorption solution of a wipe previously subjected to accelerated aging at 30 ° C for 8 weeks (desorption using a syringe as described in Example 3).

The stability of the enzyme is described in Table 4 below: Table 4 + = Good stability;-/ + = partial stability;-= insufficient stability

Biodetermination studies were performed on the desorption solution of the wipe (desorption using a syringe as described in Example 3).

The biocidal activity of the desorbed S6 solution was determined according to the standards for bactericidal activity EN 13727 and EN 16615, and EN 13624 and EN 16615 for viral activity. The results are shown in Table 5 below. Table 5

no

no

Claims (10)

A wet detergent wipe composed of a non-fabric carrier impregnated with a detergent solution. The detergent solution includes a protease, a lipase, and an amylase. The non-fabric carrier comprises polyester fibers. The wipe according to item 1 of the scope of the patent application, wherein the detergent solution does not contain glycol and / or glycerol. The wipe according to any one of claims 1 or 2, wherein the detergent solution further comprises a mannanase. The wipe according to any one of the foregoing patent applications, wherein the detergent solution further comprises one or more surfactants. The wipe according to item 4 of the scope of the patent application, wherein the one or more surfactants are selected from non-ionic surfactants, especially from polyalkoxylated fatty alcohols and amine oxides. The wipe according to any one of the foregoing patent claims, wherein the detergent solution further comprises one or more chelating agents. The wipe according to any one of the foregoing patent claims, wherein the detergent solution further comprises one or more antimicrobial agents. The wipe according to item 7 of the scope of patent application, wherein the one or more antimicrobial agents are selected from the group consisting of a quaternary ammonium salt, an amine carrying at least one C8 to C20 alkyl group, and polyhexamethyleneguanidine (PHMB) Or a salt thereof and polyaminopropylguanide (PAPB). A use of a wipe as described in any one of claims 1 to 8 of the scope of patent application, which is used to clean and / or disinfect objects with a hard surface. A method for cleaning and / or disinfecting objects with hard surfaces, such as medical instruments and devices, and work surfaces, comprising the following steps: a) Supplying any of the items 1 to 8 of the scope of the patent application The wipes described; b) the hard surface of the object is wiped with the wipe; and c) the hard surface may need to be rinsed with water, preferably demineralized water.