JPH11209795A - Detergent composition containing enzyme - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject detergent composition not deteriorating washability even by the rise of temperature within a temperature range not deactivating a protease, capable of being rinsed in a short time and capable of being provided at a low cost by combining a specific anionic surfactant with the protease. SOLUTION: This detergent composition contains (A) an enzyme and (B) a detergent-activating component. Therein, the enzyme A comprises a protease, and the detergent-activating component B comprises only an amino acid-based anionic surfactant. The component A is preferably originated from Glycyrrhiza glabra. The component B is preferably N-lauroylmethyl-β-alanine salt, or the like. The component A and the component B are compounded in a B/A weight ratio of 0.5-10. The composition can be applied to medical equipments stained with bloods, body fluids or the like, and can rapidly and effectively wash the medical equipments.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a detergent composition containing an enzyme, and more particularly to a detergent suitable for removing an organic substance such as blood adhered to a medical device, instrument or device after use thereof. is there.

[0002]

2. Description of the Related Art With the advance of medical technology, many medical tools, instruments and devices (hereinafter referred to as medical tools, etc.) are used in hospitals and clinics. Even medical devices and the like that are kept clean before use are contaminated by body fluids secreted or discharged from the human body once used. Further, when bleeding is observed from a patient while using a medical device or the like, or when a medical device or the like is used for a patient who has already bleeding, contamination by blood may be considered.

[0003] Inexpensive medical devices such as injection needles and catheters are disposable after use to prevent contamination. However, since expensive items such as endoscopes are washed and used repeatedly, effective washing for organic substances such as body fluids and blood is required.

[0004] Cleaning is a preparatory operation for sterilization and disinfection, and sufficient cleaning is required for effective sterilization and disinfection.
If the washing is insufficient, it is considered that the residual action of the organic contaminants may result in insufficient sterilization and disinfection. Most of the organic contaminants to be washed are oils and fats, and proteins and oils are easy and effective to wash with a surfactant, and can be removed with a neutral detergent. However, proteins coagulate on the surface of medical devices and the like even with the passage of time and heating at a relatively low temperature, making it difficult to wash. It is effective to use proteolytic enzymes to remove proteins. For this reason, detergents containing proteolytic enzymes have already been developed and provided to the market as both powder and liquid detergents.

[0005] It is known that the storage stability of enzymes in powder detergents that can maintain drying is good. On the other hand, in the case of a liquid detergent in a solution, the enzyme titer is reduced even with the enzyme alone, and many surfactants inactivate or reduce the enzyme titer. Measures have been taken to reduce the enzyme titer. First
Is to add a stabilizer. The second method is to coat the enzyme with microcapsules. The third method is to use a nonionic surfactant as a cleaning active ingredient.

[0006] Among them, the method of adding a stabilizer is costly, and the method of coating an enzyme with microcapsules is costly, and it is troublesome to mass-produce.
Also, in the medical field, cleaning work time should be shortened as much as possible, but if coated with microcapsules,
It also takes time for the enzyme to work.

When a nonionic surfactant is used as a cleaning active ingredient, rinsing after use may take a long time, and the cleaning operation cannot be completed in a short time. Also,
Proteolytic enzymes increase in activity with increasing temperature up to about 65 ° C., but nonionic surfactants become less turbid due to a decrease in hydrophilicity and increase detergency with increasing temperature.

[0008]

There is an anionic surfactant as a cleaning active ingredient. Anionic surfactants do not have the disadvantage that the detergency decreases with increasing temperature, but are known to have a strong tendency to deactivate or reduce enzyme titers. For this reason, combining enzymes only with anionic surfactants has not generally been performed. When using an anionic surfactant, a method of combining it with a nonionic surfactant or combining it with another substance to stabilize the enzyme has been adopted (JP-A-60-12360).
No. 0, Japanese Patent Publication No. 7-31427, Japanese Patent Publication No. 7-9
4675, JP-A-8-157871 and the like). An object of the present invention is to provide an enzyme-containing detergent that can be rinsed in a short period of time without lowering the detergency due to a rise in temperature in a temperature range in which the protease is not inactivated, and that can be provided at low cost. It is.

[0009]

Means for Solving the Problems The present inventors have studied various anionic surfactants in order to develop a detergent containing a protease and capable of being stably present. The present inventors have found that when a surfactant is combined with a proteolytic enzyme, a synergistic effect exerts a high proteolytic effect and that the enzyme activity can be stably maintained, and the present invention has been accomplished. That is, the present invention is a detergent composition containing an enzyme and a detergent active ingredient, wherein a proteolytic enzyme is used as the enzyme, and only an amino acid-based anionic surfactant is used as the detergent active ingredient.

[0010] Usually, protease is known to be inhibited in a mixed solution with a surfactant, and the inhibition by an ionic surfactant is particularly large. For this reason, it is customary that a mixture with an ionic surfactant is not stored for a long period of time without a stabilizer. However, the present inventors have found that when a protease is present together with an amino acid-based anionic surfactant, it can be stored for a long period without a stabilizer even in a mixed solution. Therefore, the detergent composition of the present invention can stably maintain enzyme activity for a long period of time not only as a powder detergent but also as a liquid detergent. Proteolytic enzymes have the effect of degrading proteins,
It can decompose contaminants such as medical devices, especially body fluids and blood. Fats and other contaminants can be removed by an amino acid-based anionic surfactant which is a cleaning active ingredient.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The protease is not particularly limited, and various known enzymes can be used. Among them, bacterial proteases are preferred. The reason is that proteolytic enzymes induced by bacteria can obtain high titers at low cost. Among the bacteria, those derived from Bacillus subtilis are preferred. In the case of a bacterial product, toxicity testing is required, but if it is based on a bacterium of the genus Bacillus, its safety has been confirmed and it is suitable as a detergent enzyme.
Proteolytic enzymes derived from Bacillus subtilis include, for example, alcalase, sabinase, esperase,
Maxatase, biobrase (all are trade names) and the like can be mentioned. However, proteolytic enzymes are not limited to those derived from bacteria, and those purified from animals, for example, can also be used. Examples of such a substance include bank creatine purified from pig pancreas.

The amino acid-based anionic surfactant can be represented by the general formula RCON (R ₁ ) R ₂ COOM. Here, R is a saturated or unsaturated hydrocarbon group having 7 to 17 carbon atoms, R ₁ is an alkyl group or hydroxyalkyl group having 1 to 4 carbon atoms, and R ₂ is an alkyl group having 1 to 3 carbon atoms. , M is Na (sodium), K (potassium) or TEA (triethanolamine).
One preferred example of the amino acid-based anionic surfactant is N-
Lauroylmethyl-β-alanine salt (C ₁₁ H ₂₃ CON
(CH ₃ ) CH ₂ CH ₂ COOM).

The detergent composition may contain various additives in addition to the protease and the amino acid-based anionic surfactant. Such additives include, for example, fragrances, dyes,
Solvents, rust inhibitors, preservatives, etc. However, a conventional stabilizer for stabilizing the enzyme activity is not added. The mixing ratio of the protease to the amino acid-based anionic surfactant is not particularly limited, but the ratio of the amino acid-based anionic surfactant to the protease is 0.5 to 0.5 by weight.
A range of 10.0 is preferred.

By using the enzyme-containing cleaning agent of the present invention for a medical device or the like contaminated with blood, body fluid, or the like, cleaning can be performed quickly and effectively. The concentration of the cleaning solution used is not particularly limited. It can be used as a stock solution, or can be used after diluting it several hundred times with water. The cleaning composition of the present invention can be used not only for medical devices and the like, but also for other uses such as dishwashing detergent.

[0015]

The enzyme-containing detergent of the present invention uses only an amino acid-based anionic surfactant as a detergent active component.
Unlike the conventional case, the detergency can be maintained even at a high temperature because the enzyme is not combined with a nonionic surfactant in order to maintain the enzyme activity.
Further, since no stabilizer is added, it is advantageous in terms of cost. When the used medical device or the like is washed with the enzyme-containing cleaning agent of the present invention, contaminants can be removed in a short time, and the cleaning which is a preparatory operation for sterilization of the medical device and the like can be effectively performed. it can. Therefore, it can contribute to the prevention of hospital-acquired infections and can further reduce the time burden on medical sites.

[0016]

EXAMPLE As a surfactant, a predetermined amount of a protease, alcalase derived from B. subtilis, and pancreatin, a protease, purified from an animal (porcine pancreas) were used as surfactants. Concentration 30
% N-lauroylmethyl-β-alanine sodium solution is converted to a pure content of sodium N-lauroylmethyl-β-alanine, and an arbitrary amount of 0 to 7.5 g is blended. Water is added thereto to make the total amount 100 g. Then, enzyme solutions having different surfactant concentrations were prepared. Table 1 shows the results obtained by measuring the enzyme activity of these enzyme solutions after 7 days.

[0017]

[Table 1]

(Enzyme activity test method) 1 cc of the enzyme solution was added to 2 cc of a 1.5% aqueous casein solution as a substrate solution, shaken, and reacted at 30 ° C for 10 minutes. Thereafter, 3 cc of a deproteinizing reagent was added to stop the reaction. As a protein removing reagent, a reagent prepared by making 27.2 g of trichloroacetic acid, 30 g of acetic acid and 27.3 g of sodium acetate into 500 cc with pure water was used. After adding the deproteinizing reagent, the mixture was kept for 20 minutes to completely precipitate the undegraded protein substrate. The solution was filtered through a filter paper No. 131. The filtrate was diluted, and the amount of casein protein decomposed was measured from the absorbance at 280 nm to determine the enzyme activity. The amount of casein proteolysis is shown in terms of tyrosine.

From the results in Table 1, it can be seen that the enzyme activity of the added sample is higher at all enzymes and at all concentrations than in the case where no N-lauroylmethyl-β-alanine sodium solution is added. That is, by adding N-lauroylmethyl-β-alanine sodium,
It can be seen that the activity of these enzymes has been improved.

The enzyme solutions having the respective compositions of the protease and sodium N-lauroylmethyl-β-alanine shown in Table 1 as well as the combinations of the proteases belonging to the present invention and the amino acid-based anionic surfactant were used. Enzyme liquids can be used as such, or with additives as needed.
It constitutes a stock solution of the cleaning composition of the present invention. These stock solutions can be used as they are, but can also be used after being diluted with water to an arbitrary magnification of several hundred times, for example, about 200 times.

0.5 g of the protease alcalase exemplified above is mixed with 49.5 g of water or various anionic surfactant solutions.
Was mixed to prepare an enzyme solution. Each enzyme solution 1.0
The resulting mixture was diluted with 9.0 g of water and kept at 45 ° C. After the exposure, the developed black-and-white film 5 mm x 35 mm was immersed, and the decomposition of the protein on the black-and-white film was observed. Table 2 shows the results.

[0022]

[Table 2] Comparative Example 1: Sodium polyoxyethylene lauryl ether sulfate solution (32%) Comparative Example 2: Sodium lauryl sulfate solution (33%) Comparative Example 3: Triethanolamine lauryl sulfate solution (40%)

From the results shown in Table 2, in the mixed solution of an anionic surfactant solution and a protease, as in the comparative example, the inhibitory effect of the anionic surfactant on the enzyme is remarkably exhibited.
-The enzyme solution of Example mixed with sodium lauroylmethyl-β-alanine solution can decompose proteins in the same short time as in the case where no anionic surfactant was added, and the protease was inhibited. You can see that it is not. Further, N-lauroylmethyl- is used as an anionic surfactant.
When using β-alanine triethanolamine,
Although the effect is inferior to that of sodium N-lauroylmethyl-β-alanine, the degree of inhibiting the protease is smaller than that of the anionic surfactant of the comparative example.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Takemori Takemori 7-4, Tsunodai, Suita-shi, Osaka D126-101 Inside Arboth Co., Ltd. (72) Inventor Koji Motonaka 7-4, Tsukumodai, Suita-shi, Osaka No. D126-101 Inside Arboth Co., Ltd. (72) Inventor Sei Korematsu 7-4, Tsunodai, Suita-shi, Osaka D126-101 Inside Arboth Co., Ltd. (72) Inventor Tamotsu Kawai 7-4, Tsukundai, Suita-shi, Osaka No. 101 Inside Alboth Co., Ltd.

Claims (2)

[Claims] 1. A detergent composition containing an enzyme and a detergent active ingredient, wherein the enzyme is a protease and the detergent active ingredient is only an amino acid-based anionic surfactant. Detergent composition. 2. The method according to claim 1, wherein the amino acid-based anionic surfactant is N
The enzyme-containing detergent composition according to claim 1, which is -lauroylmethyl-β-alanine salt.