TW201828970A - A use of an extract of germinated brown rice - Google Patents

Abstract

A use of an extract of germinated brown rice in the manufacture of a protective medicament, a healthy product or a nutrient supplement for preventing from increased body weight due to high lipid diet is disclosed. The extract of germinated brown rice is obtained by extracting a germinated brown rice sample by 90-99% ethanol at 30-40 DEG C for 10-15 hours.

Description

 Use of germinated rice extract  

The present invention relates to the use of a germinated rice extract, and more particularly to the use of a germinated rice extract for the preparation of a prophylactic, health food or nutritional supplement for weight gain caused by a high fat diet.

Workplace workers are busy, and all three meals are almost solved. In order to save time, they often choose simple and fast foods. Most of these foods contain high oil content or are fried foods (ie, high fat diet). Ingestion is not only easy to cause weight gain, but is more likely to cause cardiovascular disease, cerebrovascular disease, diabetes, arthritis, gout, and reproductive dysfunction. Derivation of diseases such as sleep disorders (somnipathy).

Generally, continuous exercise is controlled by diet to achieve weight maintenance. However, for the outer foods where the three meals are almost solved outside, it is really difficult to achieve complete changes in eating habits and diet structure. It can provide a preventive drug, health food or nutritional supplement for weight gain caused by a high-fat diet, which can reduce the possibility of weight gain without significantly changing the eating habits and diet structure.

In order to solve the above problems, the present invention provides a use of a germinated rice extract, which is an active ingredient extracted from germinated rice, which is used for preparing a prophylactic drug, a health food or a nutritional supplement for weight gain caused by a high-fat diet.

The use of the germinated rice extract of the present invention is a preventive medicine, a health food or a nutritional supplement for preparing a weight gain caused by a high-fat diet, wherein the germinated rice extract has an ethanol concentration of 90 to 99%. The ethanol aqueous solution is used as an extraction solvent to extract a germinated rice sample at a temperature of 30 to 40 ° C for 10 to 15 hours; thus, by extracting the active ingredient of the germinated rice, the high fat can be lowered even if the high fat diet is continuously taken. The problem of weight gain caused by diet, and thus it can be applied to the preparation of a prophylactic drug, a health food or a nutritional supplement for weight gain caused by a high-fat diet, which is an effect of the present invention.

The use of the germinated rice extract of the present invention, wherein the germinated rice extract is administered orally to a desired individual to prevent the high-fat diet from inhibiting the weight gain of the desired individual, for example, per kilogram per day. The desired individual is administered a dose of 0.228 to 9.15 mg to the desired individual, and the desired individual can be administered continuously for 16 weeks.

Fig. 1 is a line graph showing the effect of the germinated rice extract of the present invention on the change in body weight of a mouse fed a high-fat diet.

Fig. 2 is a bar graph showing the effect of administration of the germinated rice extract of the present invention on the body weight change of mice ingesting a high-fat diet.

The above and other objects, features and advantages of the present invention will become more <RTIgt; It can prevent the weight gain caused by eating a high-fat diet, so it can be applied to the preparation of preventive drugs, health foods or nutritional supplements for weight gain caused by high-fat diets. The germinated rice extracts are pharmaceutically acceptable. The agent or the excipient is combined to form a pharmaceutical composition, wherein the germinated rice extract can be prepared into any convenient form, such as a tablet, a capsule, a powder, a granule or a liquid, or the germinated rice is extracted. The composition is combined with other foods or beverages for oral administration to a desired individual for oral administration. For example, the germinated rice extract can be administered to a desired individual at a dose of 0.228 to 9.15 mg per day (0.228 to 9.15 mg/kg/day) per kg of the desired individual body weight, and can be administered continuously. The desired individual is 16 weeks old.

Wherein, the germinated rice extract is preferably obtained by a method comprising the steps of: providing a germinated rice sample; and extracting the germinated rice by using an aqueous solution of ethanol (concentration of ethanol: 90% to 99%) as an extraction solvent. The sample is obtained to obtain a germinated rice crude extract; and the germinated rice crude extract is concentrated to obtain the germinated rice extract.

In detail, the germinated rice sample refers to brown rice with slight buds. For example, brown rice (only the shelled rice, retaining the cortex, aleurone layer and germ) can be immersed at 30-40 ° C. It can be obtained after 20~25 hours in the water.

Next, the germinated rice sample and the extraction solvent (ie, 240-560 ml of extraction solvent per 80 g of the germinated rice sample) are mixed at a ratio of 1:3 to 1:7, and at a temperature of 30 to 40 ° C. The extraction may be repeated several times for 10 to 15 hours, so that the active ingredient enriched in the germinated rice sample can be completely dissolved in the extraction solvent, which is widely used by those having ordinary knowledge in the technical field of the invention. I will not repeat them here.

Moreover, the germinated rice extract can be obtained by concentrating, and the germinated rice extract can be obtained. By this procedure, the active ingredient of the germinated rice extract can be more concentrated, so that only a small amount of germinated rice is used for extraction. Things can work best.

In order to confirm that the germinated rice extract of the present invention can prevent weight gain caused by ingesting a high-fat diet, the following test was carried out.

(A) Preparation of germinated rice extract

In this embodiment, the Wanan organic brown rice purchased from Chishang Township of Taitung City is obtained, and the organic brown rice is washed and sterilized, and then immersed in water at 37 ° C for 22 hours to be emerged from the bud, and after continuous washing and drying, the obtained Germinated rice samples.

Next, 80 g of the germinated rice sample and 400 ml of an aqueous ethanol solution (95% ethanol concentration) were mixed, ultrasonically shaken for 30 minutes, and extracted at 37 ° C for 12 hours to obtain the germinated rice crude extract. .

Further, the germinated rice crude extract is further centrifuged (centrifuged at 4,000 rpm for 10 minutes) to obtain a supernatant, which is concentrated under reduced pressure at a temperature of 50 to 70 ° C to obtain an oil and a residue; After the residue is rinsed with the extraction solvent (95% ethanol solution in ethanol), the oil is mixed to obtain a mixture, and finally the mixture is concentrated under nitrogen at a temperature of 50 to 70 ° C to obtain the germination. Rice extract.

Through the above procedure, about 0.6 grams of germinated rice extract can be extracted per 80 grams of germinated rice sample, which contains about 29.2 milligrams of gamma-oryzanol.

(B) Effect of germinated rice extract on body weight changes in mice fed a high-fat diet

In this test, female mice of the C57BL/6J strain were selected as test animals (6 weeks old), and the aforementioned mouse strains were housed in an animal room maintained at a temperature of 25 ± 1 ° C and a humidity of 55 ± 5% (lighting time and dark time). Each is 12 hours) and is free to eat and drink.

Please refer to Table 1, the mice in each group were fed with normal feed (stardard regular diet, SRD for 1 week), and the mice in group B0 were still fed normal feed, mice in group B1~B5. Then, the high fat diet (HFD, 60% fat) was changed. The mice in the B2 and B3 groups were fed with a high-fat diet every day, and the mice in the B4 and B5 groups were The germinated rice extract is orally administered daily while feeding a high-fat diet.

Please refer to Figure 1. Compared with the weight of the first week, the percentage of weight gain of the group B0 mice fed normal diet is about 20-40%, and the group B1 is fed with a high-fat diet. The percentage increase in body weight of mice is more than 60%, indicating that the intake of a high-fat diet does cause an increase in body weight. In addition, the percentage increase in body weight of mice in group B2 to B5 fed with high-fat diet while oral administration of rice sterol or germinated rice extract was lower than that in group B1 mice without oral administration of rice germination or germinated rice extract. Percentage, indicating that the intake of rice bran alcohol or germinated rice extract can effectively reduce the weight gain caused by feeding a high-fat diet. It is worth noting that comparing the B2, B4 and B3, B5 groups of the same dose of rice sterol and germinated rice extract at the same time, it can be seen that the effect of oral germinated rice extract is significantly better than the equivalent dose of rice sterol.

In addition, please refer to Figure 2, at the 16th week, the mice in the B2~B3 group who were fed with a high-fat diet while taking oral rice sterol, the percentage of weight gain and the group B1 fed only the high-fat diet. There was no difference in mice, and the percentage increase in body weight of mice in the B4~B5 group fed with high-fat diet and oral germinated rice extract was significantly reduced. It was confirmed that the effect of oral germinated rice extract was significantly better than that of equal. Dosage of rice sterol. (# indicates statistical difference with respect to group B0, * indicates statistical difference with respect to group B1, and ‡ indicates statistical difference with respect to isodose, P < 0.05).

According to the above, oral administration of 0.5-10 mg of germinated rice extract per day for 16 weeks can effectively reduce the weight gain caused by feeding a high-fat diet, and the weight of each group of mice can be known. The dosage of the germinated rice extract is about 0.228~9.115 mg/kg/day.

In summary, the use of the germinated rice extract of the present invention is for the preparation of a prophylactic, health food or nutritional supplement for weight gain caused by a high-fat diet, wherein the germinated rice extract is 90% to 99% The ethanol aqueous solution is used as an extraction solvent to extract a germinated rice sample at a temperature of 30 to 40 ° C for 10 to 15 hours; thus, by extracting the active ingredient of the germinated rice, the high fat can be lowered even if the high fat diet is continuously taken. The problem of weight gain caused by diet, and thus it can be applied to the preparation of a prophylactic drug, a health food or a nutritional supplement for weight gain caused by a high-fat diet, which is an effect of the present invention.

Furthermore, the germinated rice extract of the present invention can further reduce the weight gain caused by the high-fat diet, thereby reducing the body-induced cardiovascular disease, cerebrovascular disease, diabetes, arthritis, gout, reproductive disorders, The risk of diseases such as sleep disorders is the efficacy of the present invention.

While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.

Claims (6)

 The use of a germinated rice extract for the preparation of a prophylactic drug for weight gain caused by a high-fat diet, wherein the germinated rice extract is an aqueous solution of ethanol having an ethanol concentration of 90 to 99% as an extraction solvent. A germinated rice sample was extracted at a temperature of ~40 ° C for 10 to 15 hours.    The use of a germinated rice extract is a health food for preparing a weight gain caused by a high-fat diet, wherein the germinated rice extract is an ethanol solution having an ethanol concentration of 90 to 99% as an extraction solvent, at 30~ A germinated rice sample was extracted at a temperature of 40 ° C for 10 to 15 hours.    The use of a germinated rice extract for the preparation of a nutritional supplement for weight gain caused by a high-fat diet, wherein the germinated rice extract is an aqueous solution of ethanol having an ethanol concentration of 90 to 99% as an extraction solvent. A germinated rice sample was extracted at a temperature of ~40 ° C for 10 to 15 hours.    The use of the germinated rice extract according to any one of claims 1 to 3, wherein the germinated rice extract is orally administered to a desired individual to prevent the inhibition of the high fat diet. The individual's weight gains.    The use of the germinated rice extract of claim 4, wherein the germinated rice extract is administered to the individual in need at a dose of 0.228 to 9.15 mg per kg of the desired individual body weight per day.    The use of the germinated rice extract of claim 5, wherein the germinated rice extract is continuously administered to the desired individual for 16 weeks.