TW201800089A - 抑制脂肪細胞分化之組成物及篩選抑制脂肪細胞分化之物質之方法 - Google Patents
抑制脂肪細胞分化之組成物及篩選抑制脂肪細胞分化之物質之方法 Download PDFInfo
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Abstract
本說明書揭露包括作為活性成分之抑制SRBC表現或活性之物質之一組成物及篩選抑制脂肪細胞分化之物質之一方法。該包括本發明一態樣作為活性成分之抑制SRBC表現或活性之物質之組成物抑制脂肪細胞分化因而可用於防止或改善肥胖。此外，篩選本發明一態樣抑制脂肪細胞分化之物質之方法藉由以一測試物質處理皮膚脂肪細胞使其能便利、快速及有效地篩選出能抑制脂肪細胞分化之物質及確認SRBC基因相對表現程度。
Description
本說明書揭露包括作為活性成分之抑制SRBC表現或活性之物質之一組成物及一用於篩選抑制脂肪細胞分化之物質之方法。
肥胖通常意指過重之狀態，但更準確意指累積過度之體脂。由於過重(包含肥胖)已知會造成多種健康問題(包含各種慢性疾病，諸如高血壓、第二型糖尿病、心血管疾病、脂肪肝、高脂血症等)，全球已對各種用於治療肥胖之藥品、天然物質等進行研究。肥胖係由積極分化之脂肪細胞誘導。據報導脂肪細胞之分化會藉外部分刺激(如激素)經由細胞内基因表現之複雜調控過程而發生。與脂肪細胞分化相關之新標誌物之研究也在進行中以更精確及有效地尋找此種物質。
[技術問題]
在一態樣中，本發明之目的在於提供一新穎之脂肪細胞分化標誌。
在另一態樣中，本發明之目的在於抑制脂肪細胞分化。
在另一態樣中，本發明之目的在於抑制脂肪細胞分化及藉此抑制肥胖。
在另一態樣中，本發明之目的在於方便地篩選出用於抑制脂肪細胞分化之物質。
[技術方案]
在一態樣中，本發明提供一抑制脂肪細胞分化之組成物，其包括作為活性成分之抑制 與C-激酶結合之血清剝奪反應因子相關之基因產物(Serum deprivation Response factor-related gene product that Binds to C-kinase，SRBC)表現或活性之物質。
在另一態樣中，本發明提供一用於防止或改善肥胖之組成物，其包括作為活性成分之抑制SRBC表現或活性之物質。
在另一態樣中，本發明提供一用於篩選抑制脂肪細胞分化之物質之方法，其包括以下步驟：以一測試物質處理脂肪細胞；及在經以該測試物質處理之皮膚細胞中確認以該測試物質處理前後之SRBC相對表現程度。
[發明功效]
該包括本發明一態樣之作為活性成分之抑制SRBC表現或活性之物質之組成物能抑制脂肪細胞分化，因而可用於抗肥胖。 此外，用於篩選本發明一態樣之抑制脂肪細胞分化物質之方法藉由以一測試物質處理脂肪細胞並確認 SRBC基因相對表現程度，從而能便利、快速及有效地篩選出抑制脂肪細胞分化之物質。
本文以下，本說明書將被詳述。
在一態樣中，本發明係一抑制脂肪細胞分化之組成物，其包括作為活性成分抑制 與C-激酶結合之血清剝奪反應因子(SRBC)表現或活性之物質。
在一態樣中，本發明係一防止或改善肥胖之組成物，其包括一作為活性成分之抑制 與C-激酶結合之血清剝奪反應因子(SRBC)表現或活性之物質。
SRBC表現及脂肪細胞分化間之關係仍未知。本案發明人發現在脂肪細胞分化過程中與脂肪細胞分化有關之過氧化物酶體增殖物活化受體γ(peroxisome proliferator-activated receptor gamma，PPAR-γ)及脂肪酸合成酶(fatty acid synthase ，FAS)及三酸甘油酯之表現正在增加時，若SRBC表現被抑制，則此等因子及三酸甘油酯之表現會減少。結果本案發明人首次發現SRBC係脂肪細胞分化之標誌。
在以上態樣中，該組成物可增加前脂肪細胞因子-1(preadipocyte factor-1，pref-1)移動至細胞核。此外，該組成物增加pref-1之表現。
在以上態樣中，該組成物可減少一或多選自於由過氧化物酶體增殖物活化受體γ(PPARγ)、脂肪酸合成酶(FAS)、硬脂醯基-CoA去飽和酶1(stearoyl-CoA desaturase 1，SCD-1)、脂肪細胞脂肪酸結合蛋白2(adipocyte fatty acid-binding protein 2)及脂聯素(adiponectin)所組成之群組之表現。
在以上態樣中，該抑制SRBC表現或活性之物質可包括RNA核酸分子。
該RNA核酸分子可包括一或多結合至SRBC之mRNA或DNA之siRNA、shRNA、crRNA及miRNA。例如，siRNA、shRNA及miRNA可結合至SRBC之mRNA，而crRNA可結合至SRBC之DNA。
siRNA、shRNA及miRNA係RNA片段，其會造成RNA干擾(RNA interference，RNAi)。 RNA干擾係指標靶mRNA被雙股RNA(double stranded RNA，dsRNA)降解之過程，因而調降特定基因之表現。
miRNA係內生性小RNA。源自不編碼蛋白之DNA之miRNA係由髮夾形轉錄而產生。miRNA會結合至標靶mRNA的3'-UTR之互補序列以引發mRNA之轉譯抑制或去安定作用，最終作為抑制標靶mRNA蛋白質合成之抑制物。此功能也被稱為RNAi。已知一個miRNA可靶向多個mRNA，且mRNA也可藉由多個miRNA調控。引發RNAi之其他RNA包括短干擾RNA(short interfering RNA，siRNA)及具髮夾結構之shRNA，其中該siRNA為短小之19-27鹼基數(mer)的RNA。通常siRNA及shRNA係藉人工引入細胞中以引發RNAi而miRNA為內生者。
在以上態樣中，該siRNA可包括各個可與此等雜合之序列或各個與此等互補之序列。
本文使用之術語「雜合(hybridizable)」意指其可結合特定單股RNA以形成雙股RNA。
crRNA指一參與CRISPR系統之RNA。crRNA互補結合該標靶序列，然後DNA切割酶(DNA cleavage enzyme)識別且結合至crRNA之結合位。然後 反活化crRNA (trans-activating crRNA， tracrRNA)結合至該crRNA之互補序列而該DNA切割酶切斷該標靶序列。該CRISPR系統可為CRISPR/Cas，例如CRISPR/Cas9或CRISPR/Cpf1。
該RNA核酸分子可被插入至一載體(vector)表現。
在一具體實施例中，該siRNA可包括一或多序列，其係選自於由SEQ ID NOS: 1至4序列及各個能與此等序列雜合之序列所組成之群組。具體而言，在一具體實施例中，該siRNA可包括SEQ ID NO: 1及SEQ ID NO: 3之序列且可包括SEQ ID NO: 2及SEQ ID NO: 4之序列。該SEQ ID NO: 1或2之序列可為有義股(sense strand)，該SEQ ID NO: 3可為該SEQ ID NO: 1序列之反義股(antisense strand)，而該SEQ ID NO: 4序列可為該SEQ ID NO: 2序列之反義股。
例如，SEQ ID NO: 1序列可包括 5'-ggagcuuuca gccuaauuut t-3'序列， SEQ ID NO: 2序列可包括5'-cccgugcuuc aaauagagattt-3'序列，SEQ ID NO: 3序列可包括5'-aaauuaggcu gaaagcucctc-3'序列，而SEQ ID NO: 4序列可包括5'-ucucuauuugaagcacgggtt-3'序列。
在以上態樣中，該組成物可包括以該組成總重為基礎0.0001至70 wt%之能抑制SRBC表現或活性之物質。例如，其數量可為不少於0.0001 wt%、不少於0.01 wt%、不少於0.1 wt%、不少於10 wt%、不少於20 wt%、不少於30 wt%、不少於40 wt%、不少於50 wt%或不少於60 wt%及不多於70 wt%、不多於60 wt%、不多於50 wt%、不多於40 wt%、不多於30 wt%、不多於20 wt%、不多於10 wt%、不多於1 wt%、不多於0.1 wt%或不多於0.001 wt%。
該美容配製物可以任何適用於局部施用之形式提供。例如，其可依以下之形式提供：溶液、水中油乳劑(emulsion)、油中水乳劑、懸浮液、固體、膠劑、粉末、糊劑、泡沫或氣溶膠組成物。此等組成物可依據本領域常用之方法製備。
該美容組成物在不會損害主要功效之範圍內可包含除上述物質外之其他成分，其可對主要功效增加協同效應。本說明書之美容組成物可包括一選自於由維生素多肽、多醣及神經鞘脂所組成之群組之物質。 且，本說明書之美容組成物可包括潤濕劑、潤滑劑、表面活性劑、UV吸收劑、防腐劑、殺菌劑、抗氧化劑、pH調控劑、有機或無機顏料、香料、冷卻劑或止汗劑。該成分數量在不對本說明書目的及效果產生不利影響之範圍內可容易地由本領域技術人員決定。其數量可為以該組成物總重為基礎之0.01-5 wt%，特別為0.01-3 wt%。
該藥學組成物可以任何適用於局部施用之形式提供。該配製物之實例包括但不限於皮膚外用之溶液、懸浮液、乳劑、膠體、貼布或噴霧。非經腸胃施用之製備實例包括不限於注射劑(injection)、滴劑(drop)、軟膏劑(ointment)、洗劑(lotion)、噴霧劑(spray)、懸浮劑、乳劑或栓劑等。此等製劑可依據本領域常用之方法製備且可進一步包括所需之表面活性劑、賦形劑、給水劑(hydrant)、乳化促進劑、懸浮劑、用於滲透壓控制之鹽或緩衝劑、著色劑、香料、穩定劑、防腐劑、抗菌劑或其他常用佐劑。
本發明一例示性具體實施例之藥學組成物中之活性成分劑量將隨著年齡、性別、受試者體重、病理狀況及其嚴重程度、給藥途徑或醫師決定而改變。據此等因素決定劑量係在本領域技術人員之知識範圍內。其日用劑量可為例如但不限於0.0001 mg/kg/day至1000 mg/kg/day，更具體為0.02 mg/kg/day至100 mg/kg/day。
該製劑之劑量取決於年齡、性別、受試者重量及症狀以及給藥方法。然而，日用劑量較佳為1.0至3.0 ml。最好每天使用1至5次持續一個月或更久。
且，本文所述之健康食品可能意指但不限於由含有正常飲食中可能缺乏或對人體有功能之營養之原料(raw material)或成分(功能原料)所製造之食品，且其藉由使人體正常機能維持或活化生理機能來維持或改善健康。該健康食品可能但不限於以片劑、膠囊、粉末、顆粒、液體、丸劑等形式製造或加工。其可能以符合法律之任何形式製造及加工。
在本發明一態樣之健康飲品組成物中，除其包含作為必要成分之預定量化合物外，其成分沒有特別限制。與普通飲料一樣，在其可能包含作為添加成分之各種調味劑或天然碳水化合物。天然碳水化合物之實例為一般糖(諸如單醣(monosaccharide)、多醣(polysaccharide)、環糊精(cyclodextrin)等)及糖醇(sugar alcohols)(諸如木糖醇(xylitol)、山梨糖醇(sorbitol)、赤藻糖醇(erythritol)等)。除上述外，可使用天然調味劑(索馬甜(thaumatin)、甜菊萃取物(stevia extract)(例如甜菊醣苷A(rebaudioside A)、甘草素(glycyrrhizin)等)及合成調味劑(糖精(saccharin)、阿斯巴甜(aspartame)等)作為調味劑。
通常，該健康食品組成物之活性成分劑量之範圍係自0.0001 mg/kg/day至約1000 mg/kg/day。更佳劑量範圍係自0.02 mg/kg/day至100 mg/kg/day。可每天以一或數次分開之劑量施用。
在另一態樣中，本發明係一美容方法，其包括以該組成物處理皮膚之步驟。在一具體實施例中，該組成物可應用於諸如腹部之脂肪部分以降低脂肪從而去除或減少不需要之脂肪。
在另一態樣中，本發明提供一篩選抑制脂肪細胞分化之物質之方法，其包括以下步驟：以一測試物質處理脂肪細胞；及在經以該測試物質處理之皮膚細胞中確認以該測試物質處理前後之SRBC相對表現程度。
在以上態樣中，該方法可進一步包括以下步驟：當經一測試物質處理後之SRBC表現程度低於經該測試物質處理前之SRBC表現程度，判斷該測試物質為抑制脂肪細胞分化之物質。例如，若經一測試物質處理之脂肪細胞中之SRBC表現程度低於未經該測試物質處理之脂肪細胞中之SRBC表現程度，判斷所施用之測試物質會抑制SRBC之表現程度。如上所述，若一施用之測試物質抑制SRBC之表現程度，判斷其為抑制脂肪細胞分化物質。
本文所使用之術語「相對表現程度」係指相較於未經一測試物質處理之脂肪細胞中SRBC表現程度之經該測試物質處理之脂肪細胞中SRBC表現程度。該表現程度可能涵蓋表現之數量及表現之性質。
在一具體實施例中，在判斷一測試物質為抑制脂肪細胞分化物質之步驟中，當經該測試物質處理後之SRBC表現程度為經該測試物質處理前之SRBC表現程度之95%或更少，該測試物質可被判斷為抑制脂肪細胞分化之物質。當經一測試物質處理後之SRBC表現程度為例如但不限於經該測試物質處理前表現程度之94%或更少、95%或更少、或96%或更少，該測試物質可被判斷為一抑制脂肪細胞分化物質。該表現程度係具統計顯著性之測量結果。該術語「具統計顯著性」係指根據生物統計學存在顯著性差異之情況，其包含在定量分析中p值小於0.05之情況。
在一具體實施例中，該SRBC表現程度基因可以已知技術確定，例如但不限於反轉錄聚合酶連鎖反應(reverse transcription polymerase chain reaction ，RT-PCR)、酵素免疫分析法(ELISA)、西方轉漬法(western blotting)或免疫轉漬法(immune blotting)。
在另一態樣中，本發明係一篩選抑制脂肪細胞分化之物質之套組，其包括脂肪細胞；及說明書，其中該說明書描述該篩選方法。
本文以下將參照實施例更詳細地描述本發明之組成和效果。然而，以下之實施例僅用於說明目的，本發明範圍不以任何方式受限於該等實施例。 [實施例]
[實施例1] 製備SRBC表現被抑制之細胞株 [實施例1-1] 細胞培養 使用6孔盤於5% CO2
培養箱中在含10%小牛血清(Gibco BRL, NY, USA)及5%青黴素鏈黴素之Dulbecco改良之Eagle培養基(Dulbecco's modified Eagle's medium，DMEM) (Lonza, MD, USA)中培養小鼠3T3-L1纖維母細胞(ATCC, CL-173)2天，使其增殖而不分化。
[實施例1-2] 細胞分化 在實施例1-1中，在6孔盤中培養該未分化之脂肪細胞至95%匯集度(confluence)。然後，在5% CO2
培養箱中在含10%胎牛血清(fetal bovine serum ，FBS)(PAA, Austria)、 10 μg/ml胰島素(Sigma- Aldrich, St. Louis, USA)、0.5 mM 3-異丁基-1-甲基黄嘌呤(3-isobutyl-1-methylxantine，IBMX) (Sigma-Aldrich, St. Louis, USA)及1 μM地塞米松(dexamethasone，DEX) (Sigma-Aldrich, St. Louis, USA)之DMEM中培養2天。此後，在補充10%FBS及10 μg/ml胰島素之DMEM中培養另3天。然後，在含10%FBS之DMEM中只培養該等細胞2天以獲得分化之脂肪細胞。
[實施例1-3] RNA萃取及cDNA合成 使用TRIzol (Gibco BRL, NY, USA)萃取RNA及使用反轉錄系統(Promega Co, WI, USA)合成cDNA。
[實施例1-4] 即時定量RT-PCR (RT-qPCR) TaqMan®探針(Life technologies, CA, USA)及master mix (Life technologies, CA, USA)係被加入至實施例1-3中合成之cDNA，各基因之表現係使用Rotor-gene 3000 (Corbett Research, AUS)來分析。
[實施例1-5] 產生SRBC表現受抑制之細胞株 使用silencer pre-designed RNAi software (Ambion, Inc. USA)由實施例1-1培養之小鼠纖維母細胞之SRBC開讀框(open reading frame ，ORF)獲得抑制表現之siSRBC序列(5'-GGAGCUUUCAGCCUAAUUUtt-3'及5'-AAAUUAGGCUGAAAGCUCCtc-3')。然後，其被插入至pSilencer2.1-U6puro質體(Ambion, Inc. Austin, USA)之BamH I/Hind III中。使用FuGene® 6 Transfection Reagent (Roche Diagnostics, IN, USA)使該合成質體被轉染至細胞中。然後以含嘌黴素(1.5 μg/ml)之培養基挑選該被轉染之細胞。
[實施例2] 抑制SRBC表現後之細胞株之油紅O染(Oil Red O Staining)及成像 製備油紅O(Oil Red O，ORO)(Sigma-Aldrich, St. Louis, USA)溶解在60%丙二醇(PG) (Santa Cruz Biotechnology, Inc., CA, USA)之原液(stock solution)以判斷小鼠中分化纖維母細胞三酸甘油酯(TG)之數量。由實施例1-1及1-2獲得未分化細胞及各分化階段之細胞，以冷磷酸鹽緩衝液(PBS) (Welgene, Daegu, Korea)沖洗兩次然後以3.7%甲醛(Sigma-Aldrich, St. Louis, USA)固定1小時。然後，以冷PBS沖洗該細胞兩次及以預製備好之ORO原液染色30分鐘。以流動水沖洗之後，以Olympus IX71 (Tokyo, Japan)成像該經染色之細胞。成像後，以100% PG溶解ORO及於450 nm測量吸收度以量化TG量。
結果顯示當SRBC表現被抑制時，脂肪細胞分化被抑制(圖1及2)。
本發明對具體實施例進行描述，對本領域技術人而言顯然地可進行各種改變及修飾而不會悖離以下申請專利範圍定義之本發明精神及範圍之情況下。
無
圖1顯示當SRBC表現被抑制脂肪細胞分化表現相關因子之變化。
圖2顯示染色結果及以裸眼觀察SRBC表現被抑制之脂肪細胞。
圖3顯示當SRBC過度表現時三酸甘油酯(triglycerol，TG)含量之測量結果。
＜110＞ 愛茉莉太平洋股份有限公司(Amorepacific corporation) ＜120＞ 抑制脂肪細胞分化之組合物及篩選抑制脂肪細胞分化之物質之方法 ＜130＞ 16P250IND ＜160＞ 4 ＜170＞ KopatentIn 2.0 ＜210＞ 1 ＜211＞ 21 ＜212＞ RNA ＜213＞ 人工序列 ＜220＞ ＜223＞ 有義股_SRBC-1 siRNA ＜400＞ 1 ggagcuuuca gccuaauuut t 21 ＜210＞ 2 ＜211＞ 22 ＜212＞ RNA ＜213＞ 人工序列 ＜220＞ ＜223＞ 有義股_SRBC-2 siRNA ＜400＞ 2 cccgugcuuc aaauagagat tt 22 ＜210＞ 3 ＜211＞ 21 ＜212＞ RNA ＜213＞ 人工序列 ＜220＞ ＜223＞ 反義股_SRBC-1 siRNA ＜400＞ 3 aaauuaggcu gaaagcucct c 21 ＜210＞ 4 ＜211＞ 21 ＜212＞ RNA ＜213＞ 人工序列 ＜220＞ ＜223＞ 反義股_SRBC-2 siRNA ＜400＞ 4 ucucuauuug aagcacgggt t 21
Claims (13)
- 一種抑制脂肪細胞分化之組成物，其包括一作為活性成分之抑制 與C-激酶結合之血清剝奪反應因子(SRBC)表現或活性之物質。
- 一種防止或改善肥胖之組成物，其包括一作為活性成分之抑制 與C-激酶結合之血清剝奪反應因子(SRBC)表現或活性之物質。
- 如請求項1或2所述之組成物， 其中該組成物增加 前脂肪細胞因子-1( pref-1)移動至細胞核或pref-1之表現。
- 如請求項1或2所述之組成物， 其中該抑制SRBC表現或活性之物質包括RNA核酸分子。
- 如請求項4所述之組成物， 其中該RNA核酸分子包括一或多siRNA、shRNA、crRNA及miRNA，其結合至SRBC之mRNA或DNA。
- 如請求項5所述之組成物， 其中該siRNA包括一或多序列，其選自於由以下所組群之群組： SEQ ID NOS: 1至4之序列及各個可與此等序列雜合之序列。
- 如請求項1或2所述之組成物， 其中該組成物包括以該組成物總重為基礎0.0001至70wt%之抑制SRBC表現或活性之物質。
- 如請求項1或2所述之組成物， 其中該組成物係一藥學組成物、一健康食品組成物或一美容組成物。
- 一種美容方法，其包括以請求項1或2所述之組成物處理皮膚之一步驟。
- 一種篩選抑制脂肪細胞分化之物質之方法，其包括以下步驟： 以一測試物質處理脂肪細胞；及 在經以該測試物質處理之皮膚細胞中確認以該測試物質處理前後之 與C-激酶結合之血清剝奪反應因子(SRBC)相對表現程度。
- 如請求項10所述之篩選抑制脂肪細胞分化之物質之方法， 其中該篩選方法進一步包括一步驟：當經該測試物質處理後之SRBC表現程度低於經該測試物質處理前之SRBC表現程度，判斷該測試物質為抑制脂肪細胞分化之物質。
- 如請求項11所述之篩選抑制脂肪細胞分化之物質之方法， 其中，在判斷該測試物質為抑制脂肪細胞分化之物質之步驟中，當經該測試物質處理後之SRBC表現程度為經該測試物質處理前之SRBC表現程度之95%或更少，該測試物質被判斷為抑制脂肪細胞分化之物質。
- 一種篩選抑制脂肪細胞分化之物質之套組，其包括脂肪細胞；及說明書，其中該說明書描述如請求項10至12所述任一者之方法。
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